keyword
https://read.qxmd.com/read/32290502/enzymatic-hydrolysis-of-marine-collagen-and-fibrinogen-proteins-in-the-presence-of-thrombin
#21
JOURNAL ARTICLE
Ludmila L Semenycheva, Marfa N Egorikhina, Victoria O Chasova, Natalya B Valetova, Yulia L Kuznetsova, Alexander V Mitin
: <b/>Enzymatic hydrolysis of native collagen and fibrinogen was carried out under comparable conditions at room temperature. The molecular weight parameters of proteins before and after hydrolysis by thrombin were monitored by gel-penetrating chromatography (GPC). An analysis of the experiment results shows that the molecular weight parameters of the initial fibrinogen (Fn) and cod collagen (CC) are very similar. High molecular CC decays within the first minute, forming two low molecular fractions. The main part (~80%) falls on the fraction with a value of Mw less than 10 kDa...
April 11, 2020: Marine Drugs
https://read.qxmd.com/read/32173431/novel-pheretima-guillelmi-derived-antithrombotic-protein-dpf3-identification-characterization-in-vitro-evaluation-and-antithrombotic-mechanisms-investigation
#22
JOURNAL ARTICLE
Yali Wu, Shaonan Hu, Yunnan Ma, Bo Zhao, Wanqing Yang, Yang Lu, Pengyue Li, Shouying Du
In this study, the antithrombotic protein, named DPf3, was purified from Pheretima guillelmi by ion-exchange chromatography. The protein pattern of DPf3 was mainly at 26-34 kDa; its two main proteins, DPf3 ID NO.1 and NO.2, were detected to be 36,121.745 Da and 24,485.004 Da consisting of 329 and 241 amino acids, respectively; the full covered protein sequences were consistent with Ac44553_g1_i1_1 and Dc43026_g1_i1_2 in our previous constructed P. guillelmi local database. The secondary structure of DPf3 is the mixture of α-helix (0...
March 12, 2020: International Journal of Biological Macromolecules
https://read.qxmd.com/read/32135225/programmable-antibiotic-delivery-to-combat-methicillin-resistant-staphylococcus-aureus-through-precision-therapy
#23
JOURNAL ARTICLE
Shaoqi Qu, Ying Liu, Qiao Hu, Yiming Han, Zhihui Hao, Jianzhong Shen, Kui Zhu
The rapid dissemination of life-threatening multidrug-resistant bacterial pathogens calls for the development of new antibacterial agents and alternative strategies. The virulence factor secreted by bacteria plays a crucial role in the sophisticated processes during infections. Inspired by the unique capacity of many bacteria inducing clotting of plasma to initiate colonization, we propose a programmable antibiotic delivery system for precision therapy using methicillin-resistant S. aureus (MRSA) as a model...
March 2, 2020: Journal of Controlled Release
https://read.qxmd.com/read/31560248/generation-of-a-monoclonal-antibody-against-d-dimer-using-hts-based-lica
#24
JOURNAL ARTICLE
Yuan Dong, Hanjin Hou, An Chen, Wei Ma, Moli Yin, Fanwei Meng, Chuanmin Hu, Huiyan Wang, Jianhui Cai
D-dimer is an essential diagnostic index of thrombotic diseases. Since the existing anti-D-dimer antibodies vary in quality and specificity, a search for alternative anti-D-dimer antibodies is required. The present study aimed to screen a novel monoclonal antibody (mAb) against D-dimer using a light-initiated chemiluminescence assay (LiCA). In this work, mice were immunized with antigen prepared from human plasma by enzyme hydrolysis. After screening, a novel mAb, DD 2G11, was obtained. The results of sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and Western blot analysis indicated that DD 2G11 could be used as a standard marker for D-dimer...
September 27, 2019: SLAS Discovery
https://read.qxmd.com/read/31413231/genome-sequencing-purification-and-biochemical-characterization-of-a-strongly-fibrinolytic-enzyme-from-bacillus-amyloliquefaciens-jxnuwx-1-isolated-from-chinese-traditional-douchi
#25
JOURNAL ARTICLE
Huilin Yang, Lin Yang, Xiang Li, Hao Li, Zongcai Tu, Xiaolan Wang
A strongly fibrinolytic enzyme was purified from Bacillus amyloliquefaciens Jxnuwx-1, found in Chinese traditional fermented black soya bean (douchi). The molecular mass of the enzyme, estimated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), was 29 kDa. The optimal pH and temperature for the enzyme were 7.6 and 41°C, respectively. The enzyme was inhibited by phenylmethylsulfonyl fluoride, soybean trypsin inhibitor, ethylenediaminetetraacetic acid, Fe3+ , and Fe2+ . The highest affinity exhibited by the enzyme was towards N-Succinyl-Ala-Ala-Pro-Phe-pNA...
August 14, 2019: Journal of General and Applied Microbiology
https://read.qxmd.com/read/31213278/a-novel-autolysin-atla-ss-mediates-bacterial-cell-separation-during-cell-division-and-contributes-to-full-virulence-in-streptococcus-suis
#26
JOURNAL ARTICLE
Yue Zhang, Xiaojun Zhong, Pengpeng Lu, Yinchu Zhu, Wenyang Dong, Shipra Roy, H M A Hejair, Zihao Pan, Jiale Ma, Huochun Yao
Streptococcus suis (SS) is a major pathogen in the swine industry, and also an important zoonotic agent for humans. The novel SS cell surface protein, AtlASS , comprising the special GW module and N-acetylmuramidases domain, was designated as a putative autolysin. Indeed, the atlASS deletion mutant almost completely lost its activity in Triton X-100 induced bacterial autolysis, while the wild-type and CΔatlASS strains showed significant decrease, to less than 20% of the initial OD600 values. Unexpectedly, both immunofluorescence and immunogold electron microscopy confirmed that AtlASS is mainly located in the cell division septum, suggesting autolytic activity in peptidoglycan hydrolysis may be required for cell separation, thus modulating and truncating bacterial chain length...
July 2019: Veterinary Microbiology
https://read.qxmd.com/read/31159882/global-serum-proteomic-changes-in-water-buffaloes-infected-with-fasciola-gigantica
#27
JOURNAL ARTICLE
Fu-Kai Zhang, Rui-Si Hu, Hany M Elsheikha, Zhao-An Sheng, Wei-Yu Zhang, Wen-Bin Zheng, Xing-Quan Zhu, Jun-Jun He
BACKGROUND: The liver fluke Fasciola gigantica modulates several signaling pathways in infected buffaloes to facilitate its survival and establishment of persistent infection. In response to the parasite invasion, buffaloes activate innate and adaptive immune responses to counter the parasite infection. To detect new proteins that might be involved in the interaction between F. gigantica and the buffaloes, and that also might serve as biomarkers for fasciolosis, we used proteomic techniques to study the serum proteome of buffaloes during F...
June 3, 2019: Parasites & Vectors
https://read.qxmd.com/read/31059753/blood-anticlotting-activity-of-a-rhipicephalus-microplus-cathepsin-l-like-enzyme
#28
JOURNAL ARTICLE
Marina Amaral Xavier, Lucas Tirloni, Ricardo Torquato, Aparecida Tanaka, Antônio F M Pinto, Jolene K Diedrich, John R Yates, Itabajara da Silva Vaz, Adriana Seixas, Carlos Termignoni
In parasites, cathepsins are implicated in mechanisms related to organism surveillance and host evasion. Some parasite cathepsins have fibrinogenolytic and fibrinolytic activity, suggesting that they may contribute to maintain blood meal fluidity for extended feeding periods. Here, it is shown that BmGTI (Rhipicephalus [Boophilus] microplus Gut Thrombin Inhibitor), a protein previously described as an inhibitor of fibrinogen hydrolysis and platelet aggregation by thrombin, and BmCL1 (Rhipicephalus [Boophilus] microplus Cathepsin-L like 1) are the same protein, hereinafter referred to using the earliest name (BmCL1)...
August 2019: Biochimie
https://read.qxmd.com/read/31047931/natural-constituents-of-st-john-s-wort-inhibit-the-proteolytic-activity-of-human-thrombin
#29
JOURNAL ARTICLE
Ling-Hua Wei, Tian-Ran Chen, Hong-Bo Fang, Qiang Jin, Shui-Jun Zhang, Jie Hou, Yang Yu, Tong-Yi Dou, Yun-Feng Cao, Wen-Zhi Guo, Guang-Bo Ge
Thrombin, a multifunctional serine protease responsible for the proteolytic hydrolysis of soluble fibrinogen, plays a pivotal role in the blood coagulation cascade. Currently, thrombin inhibitor therapy has been recognized as an effective therapeutic strategy for the prevention and treatment of thrombotic diseases. In this study, the inhibitory effects of natural constituents in St. John's Wort against human thrombin are carefully investigated by a fluorescence-based biochemical assay. The results clearly demonstrate that most of naphthodianthrones, flavonoids and biflavones exhibit strong to moderate inhibition on human thrombin...
April 29, 2019: International Journal of Biological Macromolecules
https://read.qxmd.com/read/30521914/anticoagulant-activity-of-two-novel-polysaccharides-from-flowers-of-apocynum-venetum-l
#30
JOURNAL ARTICLE
Lili Wang, Xiaofeng Zhang, Yingying Niu, Adel Fahmi Ahmed, Jinmei Wang, Wenyi Kang
Two novel polysaccharides were obtained from flowers of Apocynum venetum L., and named as Vp2a-II and Vp3. Their average molecular weights were 7 kDa and 9 kDa, respectively. Methods of monosaccharide analysis, acid hydrolysis and methylation analysis, Fourier transform-infrared spectroscopy (FT-IR), gas chromatography-mass spectrometry (GC-MS) and nuclear magnetic resonance (NMR) spectroscopy were used to identify the structure of Vp2a-II and Vp3. Vp2a-II was composed of →6)-β-d-Glcp-(1 → 6)-α-d-Galp-(1→ residues...
December 3, 2018: International Journal of Biological Macromolecules
https://read.qxmd.com/read/30181737/pharmacological-characterization-of-cnidarian-extracts-from-the-caribbean-sea-evaluation-of-anti-snake-venom-and-antitumor-properties
#31
JOURNAL ARTICLE
Cláudia S Oliveira, Cleópatra A S Caldeira, Rafaela Diniz-Sousa, Dolores L Romero, Silvana Marcussi, Laura A Moura, André L Fuly, Cicília de Carvalho, Walter L G Cavalcante, Márcia Gallacci, Maeli Dal Pai, Juliana P Zuliani, Leonardo A Calderon, Andreimar M Soares
Background: Cnidarians produce toxins, which are composed of different polypeptides that induce pharmacological effects of biotechnological interest, such as antitumor, antiophidic and anti-clotting activities. This study aimed to evaluate toxicological activities and potential as antitumor and antiophidic agents contained in total extracts from five cnidarians: Millepora alcicornis , Stichodactyla helianthus, Plexaura homomalla, Bartholomea annulata and Condylactis gigantea (total and body wall)...
2018: Journal of Venomous Animals and Toxins Including Tropical Diseases
https://read.qxmd.com/read/29909601/assessment-of-the-dual-role-of-clumping-factor-a-in-s-aureus-adhesion-to-endothelium-in-absence-and-presence-of-plasma
#32
JOURNAL ARTICLE
Jorien Claes, Bartosz Ditkowski, Laurens Liesenborghs, Tiago Rafael Veloso, Jose M Entenza, Philippe Moreillon, Thomas Vanassche, Peter Verhamme, Marc F Hoylaerts, Ruth Heying
Adhesion of Staphylococcus aureus to endothelial cells (ECs) is paramount in infective endocarditis. Bacterial proteins such as clumping factor A (ClfA) and fibronectin binding protein A (FnbpA) mediate adhesion to EC surface molecules and (sub)endothelial matrix proteins including fibrinogen (Fg), fibrin, fibronectin (Fn) and von Willebrand factor (vWF). We studied the influence of shear flow and plasma on the binding of ClfA and FnbpA (including its sub-domains A, A16+ , ABC, CD) to coverslip-coated vWF, Fg/fibrin, Fn or confluent ECs, making use of Lactococcus lactis , expressing these adhesins heterologously...
July 2018: Thrombosis and Haemostasis
https://read.qxmd.com/read/29622876/cupincin-a-novel-cupin-domain-containing-protease-from-rice-oryza-sativa-l-bran-comprising-of-procoagulant-and-fibrinogenolytic-activity
#33
JOURNAL ARTICLE
Roopesh Sreedhar, Purnima Kaul Tiku
The current study was carried out to evaluate the pharmacological properties of cupincin- A novel cupin domain containing metalloprotease with limited proteolysis from rice bran on blood coagulation and hydrolysis of human fibrinogen. Cupincin preferentially hydrolyzed the Aα chain of fibrinogen and then the Bβ-chain, but not the γ-chain. Cupincin reduced the re-calcification time of citrated human plasma dose dependently. Analysis of citrated whole blood in the presence of cupincin by rotem showed a decrease in coagulation time and clot formation time...
April 2018: Indian Journal of Hematology & Blood Transfusion
https://read.qxmd.com/read/29511577/the-relationship-between-lipoprotein-associated-phospholipase-a2-with-cardiovascular-risk-factors-in-testosterone-deficiency
#34
JOURNAL ARTICLE
Mustafa Keleşoğlu, Fuat Kızılay, Burcu Barutçuoğlu, Güneş Başol, Fulden Saraç, Işıl Mutaf, Bülent Semerci
Objective: Lipoprotein-associated phospholipase A2 (Lp-PLA2) which is believed to play a role in atherosclerotic inflammatory process due to its function in hydrolysis of phospholipids and release of pro-inflammatory products, is considered as a novel biomarker for vascular risk. In this study we aimed to investigate the alterations in Lp-PLA2 and its relationship with other cardiovascular risk factors in patients with testosterone deficiency. Material and methods: Forty hypogonadic male and 30 healthy male aged between 18-50 years were enrolled in this study...
March 2018: Turkish Journal of Urology
https://read.qxmd.com/read/29206648/relationship-between-plasma-protein-s-levels-and-apolipoprotein-c-ii-in-japanese-middle-aged-obese-women-and-young-nonobese-women
#35
JOURNAL ARTICLE
Yukiko Otsuka, Maiko Ueda, Eri Nakazono, Tomohide Tsuda, Xiuri Jin, Kenta Noguchi, Shihoko Sata, Hitomi Miyazaki, Shimako Abe, Katsumi Imai, Masako Iwamoto, Takashi Masuda, Ririko Moriguchi, Shuji Nakano, Hiroko Tsuda
: Protein S, a nonenzymatic cofactor to activated protein C, presents in two forms in plasma, free form and in a complex with C4b-binding protein. The aim of this study was to determine the association of plasma protein S levels with the variables related to cardiovascular disease risk. The relationships between plasma protein S levels with lipids, inflammation markers, and adiposity were first examined on middle-aged obese women (n = 62), then on young nonobese women (n = 160) to verify the findings in the obese women...
January 2018: Blood Coagulation & Fibrinolysis: An International Journal in Haemostasis and Thrombosis
https://read.qxmd.com/read/29075923/structural-changes-of-fibrinogen-molecule-mediated-by-the-n-homocysteinylation-reaction
#36
JOURNAL ARTICLE
Valeria Genoud, Paula G Quintana, Silvana Gionco, Alicia Baldessari, Irene Quintana
Homocysteine and its cyclic ester homocysteine thiolactone (HTL) have been involved in the detrimental consequences associated to hyperhomocysteinemia, an independent risk factor for vascular diseases. HTL reacts with protein lysine residues in a process named N-homocysteinylation. The aim of our study was to evaluate the in vitro effects of HTL on the fibrinogen through electrophoretic methods. Fibrinogen was incubated with HTL at different molar ratios and structural changes of the protein were assessed by polyacrylamide gel electrophoresis (PAGE), capillary zone electrophoresis (CZE) and capillary isoelectric focusing (CIEF)...
January 2018: Journal of Thrombosis and Thrombolysis
https://read.qxmd.com/read/28726089/oxidation-induced-modification-of-the-fibrinogen-polypeptide-chains
#37
JOURNAL ARTICLE
A V Bychkova, A D Vasilyeva, A E Bugrova, M I Indeykina, A S Kononikhin, E N Nikolaev, M L Konstantinova, M A Rosenfeld
By using the mass-spectrometry method, the oxidative modifications of the fibrinogen Aα, Bβ, and γ polypeptide chains induced by its oxidation have been studied. The αC-region has been proven to be the most vulnerable target for the oxidizer (ozone) as compared with the other structural elements of the Aα chain. The Bβ chain mapping shows that the oxidative sites are localized within all the structural elements of the chain in which the β-nodule exhibits high susceptibility to oxidation. The γ chains are the least vulnerable to the oxidizer action...
May 2017: Doklady. Biochemistry and Biophysics
https://read.qxmd.com/read/28687225/screening-cleavage-of-factor-xiii-v34x-activation-peptides-by-thrombin-mutants-a-strategy-for-controlling-fibrin-architecture
#38
JOURNAL ARTICLE
Madhavi A Jadhav, Whitney N Goldsberry, Sara E Zink, Kelsey N Lamb, Katelyn E Simmons, Carmela M Riposo, Boris A Anokhin, Muriel C Maurer
In blood coagulation, thrombin converts fibrinogen into fibrin monomers that polymerize into a clot network. Thrombin also activates Factor XIII by cleaving the R37-G38 peptide bond of the Activation Peptide (AP) segment. The resultant transglutaminase introduces covalent crosslinks into the fibrin clot. A strategy to modify clot architecture would be to design FXIII AP sequences that are easier or more difficult to be thrombin-cleaved thus controlling initiation of crosslinking. To aid in this design process, FXIII V34X (28-41) Activation Peptides were kinetically ranked for cleavage by wild-type thrombin and several anticoagulant mutants...
October 2017: Biochimica et Biophysica Acta. Proteins and Proteomics
https://read.qxmd.com/read/28648864/impact-of-plasma-protein-binding-on-cargo-release-by-thermosensitive-liposomes-probed-by-fluorescence-correlation-spectroscopy
#39
JOURNAL ARTICLE
Judith J Mittag, Barbara Kneidl, Tobias Preiβ, Martin Hossann, Gerhard Winter, Stefan Wuttke, Hanna Engelke, Joachim O Rädler
Thermosensitive liposomes (TSLs) whose phase-transition temperature (Tm ) lies slightly above body temperature are ideal candidates for controlled drug release via local hyperthermia. Recent studies, however, have revealed disruptive shifts in the release temperature Tr in mouse plasma, which are attributed to undefined interactions with blood proteins. Here, we study the effects of four major plasma proteins - serum albumin (SA), transferrin (Tf), apolipoprotein A1 (ApoA1) and fibrinogen (Fib) - on the temperature-dependent release of fluorescein di-β-D-galactopyranoside (FDG) from TSLs...
October 2017: European Journal of Pharmaceutics and Biopharmaceutics
https://read.qxmd.com/read/28192649/core-polystyrene-shell-poly-glycerol-monomethacrylate-particles
#40
JOURNAL ARTICLE
Andrew Mckenzie, Richard Hoskins, Thomas Swift, Colin Grant, Stephen Rimmer
A set of water-swollen core-shell particles was synthesized by emulsion polymerization of a 1,3-dioxolane functional monomer in water. After removal of the 1,3-dioxolane group, the particles' shells were shown to swell in aqueous media. Upon hydrolysis, the particles increased in size from around 70 to 100-130 nm. A bicinchoninic acid assay and ζ-potential measurements were used to investigate the adsorption of lysozyme, albumin, or fibrinogen. Each of the core-shell particles adsorbed significantly less protein than the noncoated core (polystyrene) particles...
March 1, 2017: ACS Applied Materials & Interfaces
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