keyword
MENU ▼
Read by QxMD icon Read
search

fibrinogen hydrolysis

keyword
https://www.readbyqxmd.com/read/29909601/assessment-of-the-dual-role-of-clumping-factor-a-in-s-aureus-adhesion-to-endothelium-in-absence-and-presence-of-plasma
#1
Jorien Claes, Bartosz Ditkowski, Laurens Liesenborghs, Tiago Rafael Veloso, Jose M Entenza, Philippe Moreillon, Thomas Vanassche, Peter Verhamme, Marc F Hoylaerts, Ruth Heying
Adhesion of Staphylococcus aureus to endothelial cells (ECs) is paramount in infective endocarditis. Bacterial proteins such as clumping factor A (ClfA) and fibronectin binding protein A (FnbpA) mediate adhesion to EC surface molecules and (sub)endothelial matrix proteins including fibrinogen (Fg), fibrin, fibronectin (Fn) and von Willebrand factor (vWF). We studied the influence of shear flow and plasma on the binding of ClfA and FnbpA (including its sub-domains A, A16+ , ABC, CD) to coverslip-coated vWF, Fg/fibrin, Fn or confluent ECs, making use of Lactococcus lactis , expressing these adhesins heterologously...
July 2018: Thrombosis and Haemostasis
https://www.readbyqxmd.com/read/29622876/cupincin-a-novel-cupin-domain-containing-protease-from-rice-oryza-sativa-l-bran-comprising-of-procoagulant-and-fibrinogenolytic-activity
#2
Roopesh Sreedhar, Purnima Kaul Tiku
The current study was carried out to evaluate the pharmacological properties of cupincin- A novel cupin domain containing metalloprotease with limited proteolysis from rice bran on blood coagulation and hydrolysis of human fibrinogen. Cupincin preferentially hydrolyzed the Aα chain of fibrinogen and then the Bβ-chain, but not the γ-chain. Cupincin reduced the re-calcification time of citrated human plasma dose dependently. Analysis of citrated whole blood in the presence of cupincin by rotem showed a decrease in coagulation time and clot formation time...
April 2018: Indian Journal of Hematology & Blood Transfusion
https://www.readbyqxmd.com/read/29511577/the-relationship-between-lipoprotein-associated-phospholipase-a2-with-cardiovascular-risk-factors-in-testosterone-deficiency
#3
Mustafa Keleşoğlu, Fuat Kızılay, Burcu Barutçuoğlu, Güneş Başol, Fulden Saraç, Işıl Mutaf, Bülent Semerci
Objective: Lipoprotein-associated phospholipase A2 (Lp-PLA2) which is believed to play a role in atherosclerotic inflammatory process due to its function in hydrolysis of phospholipids and release of pro-inflammatory products, is considered as a novel biomarker for vascular risk. In this study we aimed to investigate the alterations in Lp-PLA2 and its relationship with other cardiovascular risk factors in patients with testosterone deficiency. Material and methods: Forty hypogonadic male and 30 healthy male aged between 18-50 years were enrolled in this study...
March 2018: Turkish Journal of Urology
https://www.readbyqxmd.com/read/29206648/relationship-between-plasma-protein-s-levels-and-apolipoprotein-c-ii-in-japanese-middle-aged-obese-women-and-young-nonobese-women
#4
Yukiko Otsuka, Maiko Ueda, Eri Nakazono, Tomohide Tsuda, Xiuri Jin, Kenta Noguchi, Shihoko Sata, Hitomi Miyazaki, Shimako Abe, Katsumi Imai, Masako Iwamoto, Takashi Masuda, Ririko Moriguchi, Shuji Nakano, Hiroko Tsuda
: Protein S, a nonenzymatic cofactor to activated protein C, presents in two forms in plasma, free form and in a complex with C4b-binding protein. The aim of this study was to determine the association of plasma protein S levels with the variables related to cardiovascular disease risk. The relationships between plasma protein S levels with lipids, inflammation markers, and adiposity were first examined on middle-aged obese women (n = 62), then on young nonobese women (n = 160) to verify the findings in the obese women...
January 2018: Blood Coagulation & Fibrinolysis: An International Journal in Haemostasis and Thrombosis
https://www.readbyqxmd.com/read/29075923/structural-changes-of-fibrinogen-molecule-mediated-by-the-n-homocysteinylation-reaction
#5
Valeria Genoud, Paula G Quintana, Silvana Gionco, Alicia Baldessari, Irene Quintana
Homocysteine and its cyclic ester homocysteine thiolactone (HTL) have been involved in the detrimental consequences associated to hyperhomocysteinemia, an independent risk factor for vascular diseases. HTL reacts with protein lysine residues in a process named N-homocysteinylation. The aim of our study was to evaluate the in vitro effects of HTL on the fibrinogen through electrophoretic methods. Fibrinogen was incubated with HTL at different molar ratios and structural changes of the protein were assessed by polyacrylamide gel electrophoresis (PAGE), capillary zone electrophoresis (CZE) and capillary isoelectric focusing (CIEF)...
January 2018: Journal of Thrombosis and Thrombolysis
https://www.readbyqxmd.com/read/28726089/oxidation-induced-modification-of-the-fibrinogen-polypeptide-chains
#6
A V Bychkova, A D Vasilyeva, A E Bugrova, M I Indeykina, A S Kononikhin, E N Nikolaev, M L Konstantinova, M A Rosenfeld
By using the mass-spectrometry method, the oxidative modifications of the fibrinogen Aα, Bβ, and γ polypeptide chains induced by its oxidation have been studied. The αC-region has been proven to be the most vulnerable target for the oxidizer (ozone) as compared with the other structural elements of the Aα chain. The Bβ chain mapping shows that the oxidative sites are localized within all the structural elements of the chain in which the β-nodule exhibits high susceptibility to oxidation. The γ chains are the least vulnerable to the oxidizer action...
May 2017: Doklady. Biochemistry and Biophysics
https://www.readbyqxmd.com/read/28687225/screening-cleavage-of-factor-xiii-v34x-activation-peptides-by-thrombin-mutants-a-strategy-for-controlling-fibrin-architecture
#7
Madhavi A Jadhav, Whitney N Goldsberry, Sara E Zink, Kelsey N Lamb, Katelyn E Simmons, Carmela M Riposo, Boris A Anokhin, Muriel C Maurer
In blood coagulation, thrombin converts fibrinogen into fibrin monomers that polymerize into a clot network. Thrombin also activates Factor XIII by cleaving the R37-G38 peptide bond of the Activation Peptide (AP) segment. The resultant transglutaminase introduces covalent crosslinks into the fibrin clot. A strategy to modify clot architecture would be to design FXIII AP sequences that are easier or more difficult to be thrombin-cleaved thus controlling initiation of crosslinking. To aid in this design process, FXIII V34X (28-41) Activation Peptides were kinetically ranked for cleavage by wild-type thrombin and several anticoagulant mutants...
October 2017: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/28648864/impact-of-plasma-protein-binding-on-cargo-release-by-thermosensitive-liposomes-probed-by-fluorescence-correlation-spectroscopy
#8
Judith J Mittag, Barbara Kneidl, Tobias Preiβ, Martin Hossann, Gerhard Winter, Stefan Wuttke, Hanna Engelke, Joachim O Rädler
Thermosensitive liposomes (TSLs) whose phase-transition temperature (Tm ) lies slightly above body temperature are ideal candidates for controlled drug release via local hyperthermia. Recent studies, however, have revealed disruptive shifts in the release temperature Tr in mouse plasma, which are attributed to undefined interactions with blood proteins. Here, we study the effects of four major plasma proteins - serum albumin (SA), transferrin (Tf), apolipoprotein A1 (ApoA1) and fibrinogen (Fib) - on the temperature-dependent release of fluorescein di-β-D-galactopyranoside (FDG) from TSLs...
October 2017: European Journal of Pharmaceutics and Biopharmaceutics
https://www.readbyqxmd.com/read/28192649/core-polystyrene-shell-poly-glycerol-monomethacrylate-particles
#9
Andrew Mckenzie, Richard Hoskins, Thomas Swift, Colin Grant, Stephen Rimmer
A set of water-swollen core-shell particles was synthesized by emulsion polymerization of a 1,3-dioxolane functional monomer in water. After removal of the 1,3-dioxolane group, the particles' shells were shown to swell in aqueous media. Upon hydrolysis, the particles increased in size from around 70 to 100-130 nm. A bicinchoninic acid assay and ζ-potential measurements were used to investigate the adsorption of lysozyme, albumin, or fibrinogen. Each of the core-shell particles adsorbed significantly less protein than the noncoated core (polystyrene) particles...
March 1, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28155167/inhibition-of-snake-venom-metalloproteinase-by-%C3%AE-lactoglobulin-peptide-from-buffalo-bubalus-bubalis-colostrum
#10
Ashok Arpitha, M Sebastin Santhosh, A C Rohit, K S Girish, D Vinod, H S Aparna
Bioactive peptide research has experienced considerable therapeutic interest owing to varied physiological functions, efficacy in excretion, and tolerability of peptides. Colostrum is a rich natural source of bioactive peptides with many properties elucidated such as anti-thrombotic, anti-hypertensive, opioid, immunomodulatory, etc. In this study, a variant peptide derived from β-lactoglobulin from buffalo colostrum was evaluated for the anti-ophidian property by targeting snake venom metalloproteinases. These are responsible for rapid local tissue damages that develop after snakebite such as edema, hemorrhage, myonecrosis, and extracellular matrix degradation...
August 2017: Applied Biochemistry and Biotechnology
https://www.readbyqxmd.com/read/28057488/exploring-hemostatic-and-thrombolytic-potential-of-heynein-a-cysteine-protease-from-ervatamia-heyneana-latex
#11
Priyanka Uday, M Maheshwari, P Sharanappa, Zohara Nafeesa, Vivek Hamse Kameshwar, B S Priya, S Nanjunda Swamy
ETHNOPHARMACOLOGICAL RELEVANCE: The latex of Ervatamia heyneana (Wall.) T. Cooke plant has been used for wound healing and various skin diseases by Indian tribes and folklore. AIM OF THE STUDY: To validate the scientific basis of heynein - a key protease of Ervatamia heyneana, in hemostasis and wound healing process. MATERIALS AND METHODS: The latex from E. heyneana was processed and subjected to two step purification. The purified heynein was assayed for proteolytic activity using casein as substrate and also attested by zymography...
March 6, 2017: Journal of Ethnopharmacology
https://www.readbyqxmd.com/read/27823634/purification-and-characterization-of-novel-fibrin-ogen-olytic-protease-from-curcuma-aromatica-salisb-role-in-hemostasis
#12
B R Shivalingu, H K Vivek, B S Priya, K N Soujanya, S Nanjunda Swamy
BACKGROUND: The proteases from turmeric species have procoagulant and fibrinogenolytic activity. This provides a scientific basis for traditional use of turmeric to stop bleeding and promote wound healing processes. PURPOSE: Our previous studies revealed that fibrinogenolytic action of crude enzyme fraction of Curcuma aromatica Salisb., was found to be more influential than those of Curcuma longa L., Curcuma caesia Roxb., Curcuma amada Roxb. and Curcuma zedoria (Christm...
December 1, 2016: Phytomedicine: International Journal of Phytotherapy and Phytopharmacology
https://www.readbyqxmd.com/read/27760842/molecular-mapping-of-%C3%AE-thrombin-%C3%AE-t-%C3%AE-2-glycoprotein-i-%C3%AE-2gpi-interaction-reveals-how-%C3%AE-2gpi-affects-%C3%AE-t-functions
#13
Laura Acquasaliente, Daniele Peterle, Simone Tescari, Nicola Pozzi, Vittorio Pengo, Vincenzo De Filippis
β2-Glycoprotein I (β2GpI) is the major autoantigen in the antiphospholipid syndrome, a thrombotic autoimmune disease. Nonetheless, the physiological role of β2GpI is still unclear. In a recent work, we have shown that β2GpI selectively inhibits the procoagulant functions of human α-thrombin (αT; i.e. prolongs fibrin clotting time, tc , and inhibits αT-induced platelet aggregation) without affecting the unique anticoagulant activity of the protease, i.e. the proteolytic generation of the anticoagulant protein C (PC) from the PC zymogen, which interacts with αT exclusively at the protease catalytic site...
December 15, 2016: Biochemical Journal
https://www.readbyqxmd.com/read/27666486/agkihpin-a-novel-svtle-from-gloydius-halys-pallas-promotes-platelet-aggregation-in%C3%A2-vitro-and-inhibits-thrombus-formation-in%C3%A2-vivo-in-murine-models-of-thrombosis
#14
Huiqiong Xie, Miao Huang, Qiping Hu, Kejian Sun, Huayu Wu, Wei Shu, Xiaolong Li, Ling Fang
In previous work, a snake venom arginine esterase (SVAE), agkihpin from the venom of Gloydius halys Pallas, was isolated and its biochemical data including Mr, PI, amino acid components and sugar content was collected. Here, the agkihpin was cloned and further characterized and we found that agkihpin could promote ADP-induced platelets aggregation, hydrolyze fibrin, cleave Aα and Bβ chains of fibrinogen and reduce the thrombosis induced by thrombin. Moreover, agkihpin hydrolyzed TAME with optimum temperatures at 30 °C-45 °C, and the hydrolysis was inhibited by EDTA, PMSF, DTT and promoted by Ca(2+), Fe(3+), Mg(2+), Zn(2+)...
November 2016: Toxicon: Official Journal of the International Society on Toxinology
https://www.readbyqxmd.com/read/27134014/hydrolysis-controlled-protein-adsorption-and-antifouling-behaviors-of-mixed-charged-self-assembled-monolayer-a-molecular-simulation-study
#15
Jie Liu, Jian Zhou
UNLABELLED: Understanding the mechanism of the antimicrobial and antifouling properties of mixed charged materials is of great significance. The interactions between human gamma fibrinogen (γFg) and mixed carboxylic methyl ether-terminated (COOCH3-) and trimethylamino-terminated (N(CH3)3(+)-) SAMs and the influence of hydrolysis were studied by molecular simulations. After hydrolysis, the mixed SAMs exhibit behaviors from antimicrobial to antifouling, since the COOCH3-thiols were translated into carboxylic acid (COO(-)-) terminated thiols, which carried a net charge of -1 e...
August 2016: Acta Biomaterialia
https://www.readbyqxmd.com/read/26920259/in-vitro-antithrombotic-activities-of-peanut-protein-hydrolysates
#16
Shao Bing Zhang
The antithrombotic activities of peanut protein hydrolysates were investigated using a microplates assay. When peanut proteins were hydrolyzed to a limited extent by various enzymes, their thrombin inhibitory abilities were significantly enhanced. However, the resultant hydrolysates showed significantly different activities even at the same degrees of hydrolysis. The hydrolysates generated by Alcalase 2.4L displayed the best antithrombotic activities and the hydrolysis process was further optimized by response surface methodology...
July 1, 2016: Food Chemistry
https://www.readbyqxmd.com/read/26762094/-streptokinase-and-staphylokinase-differences-in-the-kinetics-and-mechanism-of-their-interaction-with-plasminogen-inhibitors-and-fibrin
#17
R B Aisina, L I Mukhametova, D A Gulin, K B Gershkovich, S D Varfolomeyev
Comparative in vitro study of the kinetics of various reactions involved in the process of thrombolysis initiated by streptokinase (SK) and staphylokinase (STA) was carried out. It was shown that at the interaction of an equimolar ratio of plasminogen (Pg) with SK or STA the rate of formation and the specific esterase activity of the complex plasmin (Pm) · SK are higher than those of the complex Pm · STA. The catalytic efficiency (kcat/Km) of hydrolysis of the chromogenic plasmin substrates by Pm · SK complex was 2 times higher than by Pm · STA complex...
September 2015: Bioorganicheskaia Khimiia
https://www.readbyqxmd.com/read/26578186/anticoagulant-activity-of-crude-extract-of-holotrichia-diomphalia-larvae
#18
Xueqing Xu, Wenjun Liu, Weizhen Li, Shuwen Liu
ETHNOPHARMACOLOGICAL RELEVANCE: Holotrichia diomphalia larvae are one classical folk medicinal material in East Asia which has clinically been used to promote blood circulation and dispel blood stasis for several hundred years. AIM OF THE STUDY: The anticoagulant activity of crude extract of H. diomphalia larvae (CEHDL) in vitro and in vivo was evaluated to explore its mechanism as antithrombotic medicine. MATERIALS AND METHODS: The effects of CEHDL on plasma recalcification time, platelet aggregation, bleeding time, hydrolysis of fibrinogen and fibrin were measured with normal human plasma, plasma-rich platelet, transected mouse tails and bovine fibrinogen; the anti-thrombosis activities of CEHDL in vitro and in vivo were analyzed with clots lysis assay and carrageenan-induced mouse tail thrombosis model...
January 11, 2016: Journal of Ethnopharmacology
https://www.readbyqxmd.com/read/26373334/a-bovine-fibrinogen-enriched-fraction-as-a-source-of-peptides-with-in-vitro-renin-and-angiotensin-i-converting-enzyme-inhibitory-activities
#19
Tomas Lafarga, Dilip K Rai, Paula O'Connor, Maria Hayes
Bovine fibrinogen is currently used in the food industry as a binding agent in restructured meat products. However, this protein is underused as a source of bioactive peptides. In this study, a number of novel angiotensin-I-converting enzyme (ACE-I) and renin inhibitory peptides were identified and enriched from a bovine fibrinogen fraction. Fibrinogen was isolated and enriched from bovine blood and hydrolyzed with the food-grade enzyme papain, which was selected for use using in silico analysis. The generated hydrolysate was subjected to ultrafiltration and its peptide profile characterized by liquid chromatography-tandem mass spectrometry...
October 7, 2015: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/26227411/expression-of-a-new-serine-protease-from-crotalus-durissus-collilineatus-venom-in-pichia-pastoris-and-functional-comparison-with-the-native-enzyme
#20
COMPARATIVE STUDY
Johara Boldrini-França, Renata Santos Rodrigues, Ludier Kesser Santos-Silva, Dayane Lorena Naves de Souza, Mário Sérgio Rocha Gomes, Camila Takeno Cologna, Edwin de Pauw, Loïc Quinton, Flávio Henrique-Silva, Veridiana de Melo Rodrigues, Eliane Candiani Arantes
Snake venom serine proteases (SVSPs) act primarily on plasma proteins related to blood clotting and are considered promising for the treatment of several hemostatic disorders. We report the heterologous expression of a serine protease from Crotalus durissus collilineatus, named collinein-1, in Pichia pastoris, as well as the enzymatic comparative characterization of the toxin in native and recombinant forms. The complementary DNA (cDNA) encoding collinein-1 was amplified from cDNA library of C. d. collilineatus venom gland and cloned into the pPICZαA vector...
December 2015: Applied Microbiology and Biotechnology
keyword
keyword
160277
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"