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fibrinogen degradation

Liya Jiang, Qiaohong Zhang, Wanping Xu, Yongjun Zhang
OBJECTIVE: To study the clinical phenotype and gene mutation analysis of a hereditary abnormal fibrinogenemia family and explore its molecular pathogenesis. METHODS: The STA-R automatic hemagglutination analyzer to detect the proband and its family members (3 generations of 5 people) of prothrombin time(PT), activated partial thromboplastin time (APTT), thrombin time (TT), fibrinogen activity (Fg: C), D-dimer (D-D), fibrinogen and fibrin degradation products (FDPs), plasminogen activity (PLG: A); The plasma levels of Fg: C and fibrinogen (Fg: Ag) were measured by Clauss method and immunoturbidimetry respectively...
December 10, 2018: Zhonghua Yi Xue Yi Chuan Xue za Zhi, Zhonghua Yixue Yichuanxue Zazhi, Chinese Journal of Medical Genetics
Fernanda G Amorim, Danilo L Menaldo, Sante E I Carone, Thiago A Silva, Marco A Sartim, Edwin De Pauw, Loic Quinton, Suely V Sampaio
Snake venom serine proteases (SVSPs) are enzymes that are capable of interfering in various parts of the blood coagulation cascade, which makes them interesting candidates for the development of new therapeutic drugs. Herein, we isolated and characterized Moojase, a potent coagulant enzyme from Bothrops moojeni snake venom. The toxin was isolated from the crude venom using a two-step chromatographic procedure. Moojase is a glycoprotein with N-linked glycans, molecular mass of 30.3 kDa and acidic character (pI 5...
November 28, 2018: Toxins
Yang Fu, Yumei Liu, Si Chen, Yaxiong Jin, Hong Jiang
To evaluate the correlation between the Caprini risk assessment scale and plasma thrombosis biomarkers and estimate the validity of this method in identifying critically ill patients at high risk of venous thromboembolism (VTE).Patients with VTE who were admitted to the intensive care unit (ICU) department of West China Hospital SiChuan University from October 2016 to October 2017 were enrolled in this case-control study. We retrieved relative clinical data and laboratory test results included in the Caprini risk assessment scale to calculate the Caprini score and compared thrombosis biomarkers between various risk stratifications (low, moderate, high, and highest)...
November 2018: Medicine (Baltimore)
Ruiqing Hou, Regine Willumeit-Römer, Vasil M Garamus, Marion Frant, Joachim Koll, Frank Feyerabend
Although the adsorption of proteins on Mg surface was ascribed to be the main reason for the effect of proteins on magnesium (Mg) degradation, few studies about the adsorption of proteins on Mg surface were performed due to the labile circumstance during immersion. In the present study, the adsorption of bovine serum albumin (BSA) and fibrinogen (Fib) on Mg surface during and after immersion was extensively investigated in different media for the first time. The results revealed that BSA and Fib showed a similar adsorption trend on Mg surface during and after immersion, and they adsorbed more on Mg surface in HBSS than in DMEM...
November 15, 2018: ACS Applied Materials & Interfaces
Miyuki Ookura, Naoko Hosono, Toshiki Tasaki, Kana Oiwa, Kei Fujita, Kazuhiro Ito, Shin Lee, Yasufumi Matsuda, Mihoko Morita, Katsunori Tai, Eiju Negoro, Shinji Kishi, Hiromichi Iwasaki, Takanori Ueda, Takahiro Yamauchi
Disseminated intravascular coagulation (DIC) is a life-threatening condition that frequently occurs in patients with hematologic malignancies. Currently, recombinant human soluble thrombomodulin (rTM) is a therapeutic DIC drug that is manufactured and sold in Japan only. We evaluated the efficacy of rTM compared to that of gabexate mesilate (GM), which was previously used routinely for treating DIC in Japan, in patients with acute myeloid leukemia (AML). This retrospective study enrolled 43 AML patients, including 17 with acute promyelocytic leukemia (APL), that was complicated with DIC...
November 2018: Medicine (Baltimore)
Richard W Lo, Ling Li, Richard Leung, Fred G Pluthero, Walter H A Kahr
Objective- Human and mouse megakaryocytes lacking NBEAL2 (neurobeachin-like 2) produce platelets where α-granules lack protein cargo. This cargo is mostly megakaryocyte-synthesized, but some proteins, including FGN (fibrinogen), are endocytosed. In this study, we examined the trafficking of both types of cargo within primary megakaryocytes cultured from normal and NBEAL2-null mice, to determine the role of NBEAL2 in α-granule maturation. We also examined the interaction of NBEAL2 with the granule-associated protein P-selectin in human megakaryocytes and platelets...
October 2018: Arteriosclerosis, Thrombosis, and Vascular Biology
C J Li, Z H Yang, F G Lu, X L Shi, D L Liu
BACKGROUND AND STUDY AIMS: To investigate the relationship among fibrotic, haemostatic and endotoxic changes in patients with different degrees of liver cirrhosis. PATIENTS AND METHODS: Liver fibrotic markers, including hyaluronic acid (HA), Ccollagen IV (Col-IV), laminin (LN), and N-terminal pro-peptide of collagen type III (PIIINP), were determined by radioimmunoassay. A series of haemostatic tests, including prothrombin time (PT), international normalized ratio, activated partial thromboplastin time, antithrombin-III, thrombin time, fibrinogen, fibrin(ogen) degradation product and D-dimer were determined using an automatic coagulation analyszer...
July 2018: Acta Gastro-enterologica Belgica
Pasquale Picone, Maria Antonietta Sabatino, Alessia Ajovalasit, Daniela Giacomazza, Clelia Dispenza, Marta Di Carlo
Crosslinked xyloglucan-poly(vinyl alcohol) based hydrogel films are interesting materials for wound healing applications. This work focuses on the hydrolytic degradation and consequent morphological modification of a XG-PVA film and on its interaction with cells, blood, bacteria. Biocompatibility of the film was assessed in vitro by investigating different aspects, such as cell viability, oxidative stress level, mitochondrial dysfunction and specific stress biomarkers. Partial adhesiveness was demonstrated by performing different attaching assays and phalloidin staining...
January 2019: International Journal of Biological Macromolecules
Jing-Ye Wang, Gao-Jia Zhang, Sheng-Xia Zhuo, Kai Wang, Xiao-Peng Hu, Hui Zhang, Lin-di Qu
BACKGROUND: The patterns and mechanisms underlying stroke in cancer patients differ from those of the conventional etiology. In this study, we further investigated the characteristics distinguishing cancer-associated ischemic stroke (CAIS) and the relationship of D-dimer value with CAIS. METHODS: Sixty-one acute ischemic stroke patients with cancer (cancer group) and 76 stroke patients without cancer (control group) were recruited. Cerebrovascular distribution was divided into 3 circulations and 23 vascular territories, and acute multiple brain infarcts (AMBIs) were defined as discrete MRI diffusion-weighted imaging (DWI) lesions in >1 vascular territory...
November 2018: Neurological Research
Ding Ding, Xishi Liu, Sun-Wei Guo
Our previous studies have shown that platelets play a crucial role in the development of endometriosis, and women with endometriosis appear to be in a state of hypercoagulability. However, a recent study could only replicate part of our previous finding, casting doubts on this notion. We further investigated this question through a cross-sectional study by measuring additional coagulation factors in women with and without endometriosis. To this end, we conducted a cross-sectional study of 100 women with laparoscopically and pathologically diagnosed ovarian endometriomas (OMA) and another 100 women without endometriosis...
November 2018: Reproductive Sciences
Hirobumi Fuchigami, Shino Manabe, Masahiro Yasunaga, Yasuhiro Matsumura
Cancer-induced blood coagulation in human tumour generates insoluble fibrin (IF)-rich cancer stroma in which uneven monoclonal antibody (mAb) distribution reduce the potential effectiveness of mAb-mediated treatments. Previously, we developed a mAb that reacts only with IF and not with fibrinogen (FNG) or the fibrin degradation product (FDP). Although IF, FNG and FDP share same amino acid sequences, the mAb is hardly neutralised by FNG and FDP in circulation and accumulates in fibrin clots within tumour tissue...
September 21, 2018: Scientific Reports
Toshiaki Iba, Makoto Arakawa, Yoshifumi Ohchi, Takao Arai, Koichi Sato, Hideo Wada, Jerrold H Levy
For success in clinical trials, eliminating inclusion of patients with irreversible recovery is important. The purpose of this study was to identify the patient population who do not survive for more than 3 days. A total of 449 patients with sepsis suspected of having disseminated intravascular coagulation (DIC) and treated with antithrombin were examined. The patient characteristics, baseline sequential organ failure assessment (SOFA) score, DIC score, and hemostatic markers were retrospectively analyzed in relation to early death (died within 3 days)...
September 9, 2018: Clinical and Applied Thrombosis/hemostasis
K Nellenbach, N A Guzzetta, A C Brown
Essentials The standard of care (SOC) for treating neonatal bleeding is transfusion of adult blood products. We compared neonatal clots formed with cryoprecipitate (SOC) to two procoagulant therapies. The current SOC resulted in clots with increased stiffness and decreased fibrinolytic properties. Procoagulant therapies may be a viable alternative to SOC treatment for neonatal bleeding. SUMMARY: Background Bleeding is a serious complication of neonates undergoing cardiopulmonary bypass (CPB) and associated with substantial morbidity and mortality...
November 2018: Journal of Thrombosis and Haemostasis: JTH
Kentaro Watanabe, Yuki Arakawa, Eiji Oguma, Taichi Uehara, Masato Yanagi, Chigusa Oyama, Yuhachi Ikeda, Koji Sasaki, Kiyotaka Isobe, Makiko Mori, Ryoji Hanada, Katsuyoshi Koh
Intrathecal administration of methotrexate (IT-MTX) can lead to neurotoxicity. MTX-induced neurotoxicity occasionally manifests with a stroke-like presentation that is difficult to distinguish from genuine stroke. We retrospectively reviewed records of nine patients with leukemia or lymphoma and episodes of stroke-like presentation at our institute between 2010 and 2015 for whom magnetic resonance imaging (MRI) data were available. Coagulation test results were compared between the two diagnostic groups. Four patients were diagnosed with MTX-induced stroke-like neurotoxicity...
December 2018: International Journal of Hematology
Xudong Pan, Jiakai Lu, Weiping Cheng, Yanwei Yang, Junming Zhu, Mu Jin
Background: The Stanford type A acute aortic dissection (AAD) carries a high risk of mortality and morbidity, and patients undergoing AAD surgery often bleed excessively and require blood products and transfusions. Thus, we studied how xenon alters coagulation using thromboelastography (TEG) and conventional hemostatic tests for patients with AAD undergoing aortic arch surgery involving cardiopulmonary bypass (CPB)/deep hypothermic circulatory arrest (DHCA). Methods: This prospective single-center nonrandomized controlled clinical trial, registered in the Chinese Clinical Trial Registry (ChiCTR-ICR-15006435), assessed perioperative clinical variables and serological results from 50 subjects undergoing pulmonary static inflation with 50% nitrogen/50% oxygen from January 2013 to January 2014 and 50 subjects undergoing pulmonary static inflation with 50% xenon/50% oxygen from January 2014 to December 2014 during CPB for Stanford type A AAD...
July 2018: Journal of Thoracic Disease
Yang Fu, Yumei Liu, Yaxiong Jin, Hong Jiang
BACKGROUND: Coagulation and fibrinolysis biomarkers can effectively reflect the dysfunction of coagulation and anticoagulation system, and the changes of their levels were closely related to the hypercoagulable status. The aim of this study is to study the variation tendency of these coagulation and fibrinolysis markers and explore the diagnosis power and clinical value of these biomarker for thrombosis in postoperative lung cancer patients with deep vein catheterization. METHODS: We selected 118 postoperative lung cancer patients with deep vein catheterization including 29 patients with thromboembolism and 89 patients in control group...
August 20, 2018: Zhongguo Fei Ai za Zhi, Chinese Journal of Lung Cancer
Prasad Dasari, Iordana A Shopova, Maria Stroe, Dirk Wartenberg, Hans Martin-Dahse, Niklas Beyersdorf, Peter Hortschansky, Stefanie Dietrich, Zoltán Cseresnyés, Marc Thilo Figge, Martin Westermann, Christine Skerka, Axel A Brakhage, Peter F Zipfel
The opportunistic fungal pathogen Aspergillus fumigatus can cause life-threatening infections, particularly in immunocompromised patients. Most pathogenic microbes control host innate immune responses at the earliest time, already before infiltrating host immune cells arrive at the site of infection. Here, we identify Aspf2 as the first A. fumigatus Factor H-binding protein. Aspf2 recruits several human plasma regulators, Factor H, factor-H-like protein 1 (FHL-1), FHR1, and plasminogen. Factor H contacts Aspf2 via two regions located in SCRs6-7 and SCR20...
2018: Frontiers in Immunology
Sante E I Carone, Danilo L Menaldo, Marco A Sartim, Carolina P Bernardes, Renato C Caetano, Ronivaldo R da Silva, Hamilton Cabral, Benedito Barraviera, Rui S Ferreira Junior, Suely V Sampaio
Snake venom serine proteases (SVSPs) are commonly described as capable of affecting hemostasis by interacting with several coagulation system components. In this study, we describe the isolation and characterization of BjSP from Bothrops jararaca snake venom, a serine protease with distinctive properties. This enzyme was isolated by three consecutive chromatographic steps and showed acidic character (pI 4.4), molecular mass of 28 kDa and N-carbohydrate content around 10%. Its partial amino acid sequence presented 100% identity to a serine protease cDNA clone previously identified from B...
August 24, 2018: Toxicology and Applied Pharmacology
Bahar Kharazian, Samuel E Lohse, Forough Ghasemi, Mohamad Raoufi, Amir Ata Saei, Fatemeh Hashemi, Fakhrossadat Farvadi, Reza Alimohamadi, Seyed Amir Jalali, Mohammad A Shokrgozar, Nasser L Hadipour, Mohammad Reza Ejtehadi, Morteza Mahmoudi
The surface of nanoparticles (NPs) get coated by a wide range of biomolecules, upon exposure to biological fluids. It is now being increasingly accepted that NPs with particular physiochemical properties have a capacity to induce conformational changes to proteins and therefore influence their biological fates, we hypothesized that the gold NP's metal surface may also be involved in the observed Fg unfolding and inflammatory response. To mechanistically test this hypothesis, we probed the interaction of Fg with gold surfaces using molecular dynamic simulation (MD) and revealed that the gold surface has a capacity to induce Fg conformational changes in favor of inflammation response...
August 22, 2018: Scientific Reports
Francisco Martín-Saavedra, Clara Escudero-Duch, Martín Prieto, Silvia Sánchez-Casanova, Daniel López, Manuel Arruebo, Richard Voellmy, Jesús Santamaría, Nuria Vilaboa
CuS nanoparticles (CuSNP) are degradable, readily prepared, inexpensive to produce and efficiently cleared from the body. In this work, we explored the feasibility of CuSNP to function as degradable near infrared (NIR) nanotransducers within fibrin-based cellular scaffolds. To prepare NIR-responsive CuSNP hydrogels, fibrinogen was dissolved in cell culture medium and supplemented with aqueous dispersions of CuSNP. Fibrinogen polymerization was catalyzed by the addition of thrombin. In some experiments, HUVEC, C3H/10T1/2 or C3H/10T1/2-fLuc cells, that harbor a heat-activated and rapamycin-dependent gene switch for regulating the expression of firefly luciferase transgene, were incorporated to the sol phase of the hydrogel...
September 15, 2018: Acta Biomaterialia
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