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fibrinogen degradation

Jing-Ye Wang, Gao-Jia Zhang, Sheng-Xia Zhuo, Kai Wang, Xiao-Peng Hu, Hui Zhang, Lin-di Qu
BACKGROUND: The patterns and mechanisms underlying stroke in cancer patients differ from those of the conventional etiology. In this study, we further investigated the characteristics distinguishing cancer-associated ischemic stroke (CAIS) and the relationship of D-dimer value with CAIS. METHODS: Sixty-one acute ischemic stroke patients with cancer (cancer group) and 76 stroke patients without cancer (control group) were recruited. Cerebrovascular distribution was divided into 3 circulations and 23 vascular territories, and acute multiple brain infarcts (AMBIs) were defined as discrete MRI diffusion-weighted imaging (DWI) lesions in >1 vascular territory...
November 2018: Neurological Research
Ding Ding, Xishi Liu, Sun-Wei Guo
Our previous studies have shown that platelets play a crucial role in the development of endometriosis, and women with endometriosis appear to be in a state of hypercoagulability. However, a recent study could only replicate part of our previous finding, casting doubts on this notion. We further investigated this question through a cross-sectional study by measuring additional coagulation factors in women with and without endometriosis. To this end, we conducted a cross-sectional study of 100 women with laparoscopically and pathologically diagnosed ovarian endometriomas (OMA) and another 100 women without endometriosis...
November 2018: Reproductive Sciences
Hirobumi Fuchigami, Shino Manabe, Masahiro Yasunaga, Yasuhiro Matsumura
Cancer-induced blood coagulation in human tumour generates insoluble fibrin (IF)-rich cancer stroma in which uneven monoclonal antibody (mAb) distribution reduce the potential effectiveness of mAb-mediated treatments. Previously, we developed a mAb that reacts only with IF and not with fibrinogen (FNG) or the fibrin degradation product (FDP). Although IF, FNG and FDP share same amino acid sequences, the mAb is hardly neutralised by FNG and FDP in circulation and accumulates in fibrin clots within tumour tissue...
September 21, 2018: Scientific Reports
Toshiaki Iba, Makoto Arakawa, Yoshifumi Ohchi, Takao Arai, Koichi Sato, Hideo Wada, Jerrold H Levy
For success in clinical trials, eliminating inclusion of patients with irreversible recovery is important. The purpose of this study was to identify the patient population who do not survive for more than 3 days. A total of 449 patients with sepsis suspected of having disseminated intravascular coagulation (DIC) and treated with antithrombin were examined. The patient characteristics, baseline sequential organ failure assessment (SOFA) score, DIC score, and hemostatic markers were retrospectively analyzed in relation to early death (died within 3 days)...
September 9, 2018: Clinical and Applied Thrombosis/hemostasis
Kimberly Nellenbach, Nina A Guzzetta, Ashley C Brown
BACKGROUND: Bleeding is a serious complication of neonates undergoing cardiopulmonary bypass (CPB) and associated with substantial morbidity and mortality. Bleeding is addressed through the transfusion of adult blood products including platelets and cryoprecipitate. However, significant differences exist between neonatal and adult clotting components, specifically fibrinogen. Our recent ex vivo studies have shown that neonatal fibrinogen does not fully integrate with adult fibrinogen, leading to decreased susceptibility to fibrinolysis...
September 4, 2018: Journal of Thrombosis and Haemostasis: JTH
Kentaro Watanabe, Yuki Arakawa, Eiji Oguma, Taichi Uehara, Masato Yanagi, Chigusa Oyama, Yuhachi Ikeda, Koji Sasaki, Kiyotaka Isobe, Makiko Mori, Ryoji Hanada, Katsuyoshi Koh
Intrathecal administration of methotrexate (IT-MTX) can lead to neurotoxicity. MTX-induced neurotoxicity occasionally manifests with a stroke-like presentation that is difficult to distinguish from genuine stroke. We retrospectively reviewed records of nine patients with leukemia or lymphoma and episodes of stroke-like presentation at our institute between 2010 and 2015 for whom magnetic resonance imaging (MRI) data were available. Coagulation test results were compared between the two diagnostic groups. Four patients were diagnosed with MTX-induced stroke-like neurotoxicity...
September 4, 2018: International Journal of Hematology
Xudong Pan, Jiakai Lu, Weiping Cheng, Yanwei Yang, Junming Zhu, Mu Jin
Background: The Stanford type A acute aortic dissection (AAD) carries a high risk of mortality and morbidity, and patients undergoing AAD surgery often bleed excessively and require blood products and transfusions. Thus, we studied how xenon alters coagulation using thromboelastography (TEG) and conventional hemostatic tests for patients with AAD undergoing aortic arch surgery involving cardiopulmonary bypass (CPB)/deep hypothermic circulatory arrest (DHCA). Methods: This prospective single-center nonrandomized controlled clinical trial, registered in the Chinese Clinical Trial Registry (ChiCTR-ICR-15006435), assessed perioperative clinical variables and serological results from 50 subjects undergoing pulmonary static inflation with 50% nitrogen/50% oxygen from January 2013 to January 2014 and 50 subjects undergoing pulmonary static inflation with 50% xenon/50% oxygen from January 2014 to December 2014 during CPB for Stanford type A AAD...
July 2018: Journal of Thoracic Disease
Yang Fu, Yumei Liu, Yaxiong Jin, Hong Jiang
BACKGROUND: Coagulation and fibrinolysis biomarkers can effectively reflect the dysfunction of coagulation and anticoagulation system, and the changes of their levels were closely related to the hypercoagulable status. The aim of this study is to study the variation tendency of these coagulation and fibrinolysis markers and explore the diagnosis power and clinical value of these biomarker for thrombosis in postoperative lung cancer patients with deep vein catheterization. METHODS: We selected 118 postoperative lung cancer patients with deep vein catheterization including 29 patients with thromboembolism and 89 patients in control group...
August 20, 2018: Zhongguo Fei Ai za Zhi, Chinese Journal of Lung Cancer
Prasad Dasari, Iordana A Shopova, Maria Stroe, Dirk Wartenberg, Hans Martin-Dahse, Niklas Beyersdorf, Peter Hortschansky, Stefanie Dietrich, Zoltán Cseresnyés, Marc Thilo Figge, Martin Westermann, Christine Skerka, Axel A Brakhage, Peter F Zipfel
The opportunistic fungal pathogen Aspergillus fumigatus can cause life-threatening infections, particularly in immunocompromised patients. Most pathogenic microbes control host innate immune responses at the earliest time, already before infiltrating host immune cells arrive at the site of infection. Here, we identify Aspf2 as the first A. fumigatus Factor H-binding protein. Aspf2 recruits several human plasma regulators, Factor H, factor-H-like protein 1 (FHL-1), FHR1, and plasminogen. Factor H contacts Aspf2 via two regions located in SCRs6-7 and SCR20...
2018: Frontiers in Immunology
Sante E I Carone, Danilo L Menaldo, Marco A Sartim, Carolina P Bernardes, Renato C Caetano, Ronivaldo R da Silva, Hamilton Cabral, Benedito Barraviera, Rui S Ferreira Junior, Suely V Sampaio
Snake venom serine proteases (SVSPs) are commonly described as capable of affecting hemostasis by interacting with several coagulation system components. In this study, we describe the isolation and characterization of BjSP from Bothrops jararaca snake venom, a serine protease with distinctive properties. This enzyme was isolated by three consecutive chromatographic steps and showed acidic character (pI 4.4), molecular mass of 28 kDa and N-carbohydrate content around 10%. Its partial amino acid sequence presented 100% identity to a serine protease cDNA clone previously identified from B...
August 24, 2018: Toxicology and Applied Pharmacology
Bahar Kharazian, Samuel E Lohse, Forough Ghasemi, Mohamad Raoufi, Amir Ata Saei, Fatemeh Hashemi, Fakhrossadat Farvadi, Reza Alimohamadi, Seyed Amir Jalali, Mohammad A Shokrgozar, Nasser L Hadipour, Mohammad Reza Ejtehadi, Morteza Mahmoudi
The surface of nanoparticles (NPs) get coated by a wide range of biomolecules, upon exposure to biological fluids. It is now being increasingly accepted that NPs with particular physiochemical properties have a capacity to induce conformational changes to proteins and therefore influence their biological fates, we hypothesized that the gold NP's metal surface may also be involved in the observed Fg unfolding and inflammatory response. To mechanistically test this hypothesis, we probed the interaction of Fg with gold surfaces using molecular dynamic simulation (MD) and revealed that the gold surface has a capacity to induce Fg conformational changes in favor of inflammation response...
August 22, 2018: Scientific Reports
Francisco Martín-Saavedra, Clara Escudero-Duch, Martín Prieto, Silvia Sánchez-Casanova, Daniel López, Manuel Arruebo, Richard Voellmy, Jesús Santamaría, Nuria Vilaboa
CuS nanoparticles (CuSNP) are degradable, readily prepared, inexpensive to produce and efficiently cleared from the body. In this work, we explored the feasibility of CuSNP to function as degradable near infrared (NIR) nanotransducers within fibrin-based cellular scaffolds. To prepare NIR-responsive CuSNP hydrogels, fibrinogen was dissolved in cell culture medium and supplemented with aqueous dispersions of CuSNP. Fibrinogen polymerization was catalyzed by the addition of thrombin. In some experiments, HUVEC, C3H/10T1/2 or C3H/10T1/2-fLuc cells, that harbor a heat-activated and rapamycin-dependent gene switch for regulating the expression of firefly luciferase transgene, were incorporated to the sol phase of the hydrogel...
September 15, 2018: Acta Biomaterialia
Barbara M Zietek, Morwarid Mayar, Julien Slagboom, Ben Bruyneel, Freek J Vonk, Govert W Somsen, Nicholas R Casewell, Jeroen Kool
To better understand envenoming and to facilitate the development of new therapies for snakebite victims, rapid, sensitive, and robust methods for assessing the toxicity of individual venom proteins are required. Metalloproteinases comprise a major protein family responsible for many aspects of venom-induced haemotoxicity including coagulopathy, one of the most devastating effects of snake envenomation, and is characterized by fibrinogen depletion. Snake venoms are also known to contain anti-fibrinolytic agents with therapeutic potential, which makes them a good source of new plasmin inhibitors...
September 2018: Analytical and Bioanalytical Chemistry
Peter Lin, Tsung-Liang Chuang, Paul Z Chen, Chii-Wann Lin, Frank X Gu
Surface fouling remains an exigent issue for many biological implants. Unwanted solutes adsorb to reduce device efficiency and hasten degradation while increasing the risks of microbial colonization and adverse inflammatory response. To address unwanted fouling in modern implants in vivo, surface modification with antifouling polymers has become indispensable. Recently, zwitterionic self-assembled monolayers, which contain two or more charged functional groups but are electrostatically neutral and form highly hydrated surfaces, have been the focus of many antifouling coatings...
August 13, 2018: Langmuir: the ACS Journal of Surfaces and Colloids
Yusuke S Hori, Yuki Ebisudani, Mizuho Aoi, Toru Fukuhara
BACKGROUND: Previous studies reported an association of hematologic parameters, including white blood cells, neutrophil, eosinophils, or coagulation-related factors, with prognosis in cerebrovascular disorders. However, an association of recurrence rate with serum coagulation-related factors (e.g., D-dimer or fibrinogen degradation products [FDP]) in chronic subdural hematoma (CSDH) is unclear. METHODS: Ninety-two patients who experienced first-time CSDH treated with burr-hole hematoma evacuation were included in this study...
October 2018: World Neurosurgery
Naihao Lu, Yinhua Sui, Yun Ding, Rong Tian, Yi-Yuan Peng
Carbon nanotubes are widely used in the area of biomedicine, and the binding of protein to carbon nanotubes are believed to play an important role in the potential cytotoxicity of nanomaterials. In this work, we investigated the effects of human fibrinogen-surface coatings on the biodegradation and cytotoxicity of carboxylated single-walled carbon nanotubes (SWCNTs). It was found that the electrostatic and π-π stacking interactions might be the crucial factors in stabilizing the binding of fibrinogen with SWCNTs by both theoretical and experimental approaches...
July 17, 2018: Journal of Materials Science. Materials in Medicine
Sonja E M Boas, Joao Carvalho, Marloes van den Broek, Ester M Weijers, Marie-José Goumans, Pieter Koolwijk, Roeland M H Merks
In experimental assays of angiogenesis in three-dimensional fibrin matrices, a temporary scaffold formed during wound healing, the type and composition of fibrin impacts the level of sprouting. More sprouts form on high molecular weight (HMW) than on low molecular weight (LMW) fibrin. It is unclear what mechanisms regulate the number and the positions of the vascular-like structures in cell cultures. To address this question, we propose a mechanistic simulation model of endothelial cell migration and fibrin proteolysis by the plasmin system...
July 2018: PLoS Computational Biology
Zheng Chen, Dong-Hua Shao, Zu-Min Mao, Lei-Lei Shi, Xiao-Dong Ma, Da-Peng Zhang
BACKGROUND: Dexmedetomidine can inhibit the perioperative stress response, which plays an important role in postoperative hypercoagulability. This study aimed to investigate whether dexmedetomidine could attenuate the activation of postoperative coagulation. METHODS: Patients undergoing open radical gastrectomy under total intravenous anesthesia were randomly allocated to the control group (group Con) and the dexmedetomidine group (group Dex). Dexmedetomidine was intravenously infused at 0...
July 2018: Medicine (Baltimore)
Keita Sadatani, Kenji Niiya, Hiroshi Sonobe, Kazunori Sasaki, Ichiyo Miyamoto, Mari Nakano, Toshiyuki Habara, Toru Kiguchi
We report a unique case of a B-cell lymphoma patient in whom IgM monoclonal gammopathy resulted in a prolonged activated partial thromboplastin time (APTT) and false-positive results for fibrinogen and fibrin degradation products (FDPs). An 86-year-old man was referred to our hospital for further examination of abnormal cells in his peripheral blood. Laboratory data upon admission revealed an elevation of monoclonal IgM, presence of FDPs and marked prolongation of APTT (>180 s). Bone marrow examination revealed a predominant involvement of B lymphoma cells...
May 2018: Annals of Clinical and Laboratory Science
Kristian A Torres-Bonilla, Débora Andrade-Silva, Solange M T Serrano, Stephen Hyslop
In this work, we examined the proteolytic and phospholipase A2 (PLA2 ) activities of venom from the opisthoglyphous colubrid Pseudoboa neuwiedii. Proteolytic activity (3 and 10 μg of venom) was comparable to that of Bothrops neuwiedii venom but less than Bothrops atrox. This activity was inhibited by EDTA and 1,10-phenanthroline but only slightly affected (≤30% inhibition) by PMSF and AEBSF, indicating it was mediated by snake venom metalloproteinases (SVMPs). The pH and temperature optima for proteolytic activity were 8...
November 2018: Comparative Biochemistry and Physiology. Toxicology & Pharmacology: CBP
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