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fibrinogen gel electrophoresis

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https://www.readbyqxmd.com/read/29963784/potential-plasma-biomarkers-of-bladder-cancer-identified-by-proteomic-analysis-a-pilot-study
#1
Anna Lemańska-Perek, Jolanta Lis-Kuberka, Adam Lepczyński, Alicja Dratwa-Chałupnik, Krzysztof Tupikowski, Iwona Kątnik-Prastowska, Małgorzata Ożgo
BACKGROUND: Bladder cancer diagnosis and surveillance includes cystoscopy and cytology. New methods for the detection of bladder cancer are needed, because cystoscopy is invasive and expensive, and because urine cytology is not sensitive enough. OBJECTIVES: The aim of the study was to select potential plasma protein markers for bladder cancer which could be useful in developing a specific laboratory test to improve diagnosis and to establish treatment strategies in order to prevent the recurrence of the disease...
July 2, 2018: Advances in Clinical and Experimental Medicine: Official Organ Wroclaw Medical University
https://www.readbyqxmd.com/read/29872262/effects-of-hemocoagulase-agkistrodon-on-the-coagulation-factors-and-its-procoagulant-activities
#2
Haixin Li, Ying Huang, Xian Wu, Ting Wu, Ying Cao, Qimei Wang, Yuchang Qiu, Weiming Fu, Qun Zhang, Jianxin Pang
Objective: Hemocoagulase agkistrodon (HCA), a thrombin-like enzyme (TLE) from the venom of the Chinese moccasin snake ( Deinagkistrodon acutus ), has been used in clinical practice as a hemostatic compound. The aim of this study was to further investigate the pharmacological properties of HCA. Materials and methods: Sodium dodecyl sulfate or native polyacrylamide gel electrophoresis (SDS- or N-PAGE) as well as enzyme linked immunosorbent assays (ELISAs) were conducted to study the effects of HCA on the human plasma fibrinogen and prothrombin levels, as well as its in vitro interactions with some coagulation factors...
2018: Drug Design, Development and Therapy
https://www.readbyqxmd.com/read/29544770/acclimation-to-cold-and-warm-temperatures-is-associated-with-differential-expression-of-male-carp-blood-proteins-involved-in-acute-phase-and-stress-responses-and-lipid-metabolism
#3
Mariola A Dietrich, Piotr Hliwa, Mikołaj Adamek, Dieter Steinhagen, Halina Karol, Andrzej Ciereszko
The environmental temperature affects plasma biochemical indicators, antioxidant status and hematological and immunological parameters in fish. So far, only single blood proteins have been identified in response to temperature changes. The aim of this study was to compare the proteome of carp blood plasma from males acclimated to warm (30 °C) and cold (10 °C) temperatures by two-dimensional differential gel electrophoresis followed by MALDI-TOF/TOF mass spectrometry. A total of 47 spots were found to be differentially regulated by temperature (>1...
May 2018: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/29534707/putative-salivary-protein-biomarkers-for-the-diagnosis-of-oral-lichen-planus-a-case-control-study
#4
Sineepat Talungchit, Waranun Buajeeb, Chotima Lerdtripop, Rudee Surarit, Kongthawat Chairatvit, Sittiruk Roytrakul, Hiroaki Kobayashi, Yuichi Izumi, Siribang-On Piboonniyom Khovidhunkit
BACKGROUND: Salivary protein biomarkers for screening and diagnosis of oral lichen planus (OLP) are not well-defined. The objective of this study was to identify putative protein biomarkers for OLP using proteomic approaches. METHODS: Pooled unstimulated whole saliva was collected from five OLP patients and five healthy control participants. Saliva samples were then subjected to two-dimensional gel electrophoresis, followed by mass spectrometry to identify putative protein biomarkers...
March 13, 2018: BMC Oral Health
https://www.readbyqxmd.com/read/29518939/quantitative-proteomic-approach-targeted-to-fibrinogen-%C3%AE-chain-in-tissue-gastric-carcinoma
#5
Ombretta Repetto, Stefania Maiero, Raffaella Magris, Gianmaria Miolo, Maria Rita Cozzi, Agostino Steffan, Vincenzo Canzonieri, Renato Cannizzaro, Valli De Re
Elevated plasma fibrinogen levels and tumor progression in patients with gastric cancer (GC) have been largely reported. However, distinct fibrinogen chains and domains have different effects on coagulation, inflammation, and angiogenesis. The aim of this study was to characterize fibrinogen β chain (FGB) in GC tissues. Retrospectively we analyzed the data of matched pairs of normal (N) and malignant tissues (T) of 28 consecutive patients with GC at diagnosis by combining one- and two-dimensional electrophoresis (1DE and 2DE) with immunoblotting and mass spectrometry together with two-dimensional difference in gel electrophoresis (2D-DIGE)...
March 7, 2018: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/29332824/dithiothreitol-based-protein-equalization-technology-to-unravel-biomarkers-for-bladder-cancer
#6
J E Araújo, H López-Fernández, M S Diniz, Pedro M Baltazar, Luís Campos Pinheiro, Fernando Calais da Silva, Mylène Carrascal, Paula Videira, H M Santos, J L Capelo
This study aimed to assess the benefits of dithiothreitol (DTT)-based sample treatment for protein equalization to assess potential biomarkers for bladder cancer. The proteome of plasma samples of patients with bladder carcinoma, patients with lower urinary tract symptoms (LUTS) and healthy volunteers, was equalized with dithiothreitol (DTT) and compared. The equalized proteomes were interrogated using two-dimensional gel electrophoresis and matrix assisted laser desorption ionization time of flight mass spectrometry...
April 1, 2018: Talanta
https://www.readbyqxmd.com/read/29312627/a-proteomic-approach-for-the-identification-of-biomarkers-in-endometrial-cancer-uterine-aspirate
#7
Blendi Ura, Lorenzo Monasta, Giorgio Arrigoni, Cinzia Franchin, Oriano Radillo, Isabel Peterlunger, Giuseppe Ricci, Federica Scrimin
Endometrial cancer arises from the endometrium. It has a slow progression and a reported survival rate of 75%. The identification of soluble biomarkers in the uterine aspirate may be very useful for its early diagnosis. Uterine aspirates from 10 patients with endometrial cancer and 6 non-endometrial cancer controls were analyzed by two-dimensional gel electrophoresis coupled with mass spectrometry and western blotting for data verification. A total of 25 proteins with fold change in %V ≥2 or ≤0.5 in intensity were observed to change significantly (P<0...
December 12, 2017: Oncotarget
https://www.readbyqxmd.com/read/29301248/differences-in-the-plasma-proteome-of-patients-with-hypothyroidism-before-and-after-thyroid-hormone-replacement-a-proteomic-analysis
#8
Assim A Alfadda, Hicham Benabdelkamel, Afshan Masood, Anwar A Jammah, Aishah A Ekhzaimy
Thyroid hormone is a potent stimulator of metabolism, playing a critical role in regulating energy expenditure and in key physiological mechanisms, such as growth and development. Although administration of thyroid hormone in the form of levo thyroxine (l-thyroxine) has been used to treat hypothyroidism for many years, the precise molecular basis of its physiological actions remains uncertain. Our objective was to define the changes in circulating protein levels that characterize alterations in thyroid hormone status...
January 1, 2018: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/29173582/staphylococcus-agnetis-a-potential-pathogen-in-broiler-breeders
#9
Louise Ladefoged Poulsen, Ida Thøfner, Magne Bisgaard, Rikke Heidemann Olsen, Jens Peter Christensen, Henrik Christensen
In this study, four broiler parent flocks have been followed from the onset of the production period (week 20) until slaughter (week 60). Every week, approximately ten dead broiler breeders, randomly selected among birds dead on their own, were collected and subjected to a full post mortem analysis including bacteriological examination. In total 997 breeders were investigated and for the first time Staphylococcus agnetis was isolated in pure culture from cases of endocarditis and septicemia from 16 broiler breeders...
December 2017: Veterinary Microbiology
https://www.readbyqxmd.com/read/29126301/coagulation-testing-in-the-core-laboratory
#10
REVIEW
William E Winter, Sherri D Flax, Neil S Harris
Primary hemostasis begins with endothelial injury. VWF, produced by endothelial cells, binds to platelets and links them to subendothelial collagen. Platelet-derived ADP and thromboxane activate non-adhered platelets via their GPIIb/IIIa receptors, allowing these platelets to participate in platelet aggregation. Secondary hemostasis is initiated with the binding of factor VII to extravascular tissue factor (TF). Factors II, VII, IX and X are vitamin K-dependent factors. The role of vitamin K is to assist in the addition of gamma carboxylate groups to glutamic acids in the "GLA" domains of these factors...
November 8, 2017: Laboratory Medicine
https://www.readbyqxmd.com/read/29080870/-the-range-and-potential-contribution-of-irreversibly-adsorbed-proteins-on-the-surface-of-artificial-implants
#11
J V Ponomareva, L V Limareva, M N Milyakova
Protein adsorption is the first stage of the interaction between prosthetic materials with tissues of the body. They undergo conformational changes depending on the chemical composition and the nanotopography surface. Adsorbed proteins induce adhesion and alter the functional state of migrating cells. Plasma samples from patients were incubated with such matrices as titanium, polypropylene or polyester with fluoropolymer coating meshes. Bound peptides were analyzed by electrophoresis. Qualitative analysis of the peptides extracted from the gel was performed by chromatography-mass spectrometry...
October 2017: Biomedit︠s︡inskai︠a︡ Khimii︠a︡
https://www.readbyqxmd.com/read/29075923/structural-changes-of-fibrinogen-molecule-mediated-by-the-n-homocysteinylation-reaction
#12
Valeria Genoud, Paula G Quintana, Silvana Gionco, Alicia Baldessari, Irene Quintana
Homocysteine and its cyclic ester homocysteine thiolactone (HTL) have been involved in the detrimental consequences associated to hyperhomocysteinemia, an independent risk factor for vascular diseases. HTL reacts with protein lysine residues in a process named N-homocysteinylation. The aim of our study was to evaluate the in vitro effects of HTL on the fibrinogen through electrophoretic methods. Fibrinogen was incubated with HTL at different molar ratios and structural changes of the protein were assessed by polyacrylamide gel electrophoresis (PAGE), capillary zone electrophoresis (CZE) and capillary isoelectric focusing (CIEF)...
January 2018: Journal of Thrombosis and Thrombolysis
https://www.readbyqxmd.com/read/29031222/apolipoproteins-adsorption-and-brain-targeting-evaluation-of-baicalin-nanocrystals-modified-by-combination-of-tween80-and-tpgs
#13
Yang Liu, Yueqin Ma, Junnan Xu, Yingchong Chen, Jin Xie, Pengfei Yue, Qin Zheng, Ming Yang
To help baicalin pass across BBB and improve its targeting in brain, we designed a novel formulation strategy of baicalin nanocrystals that preferentially adsorbing apolipoprotein E (ApoE) and repelling protein adsorption of opsonins. Intravenous baicalin nanocrystals suspensions (BCL-NS) modified by different surfactant were prepared by high-pressure homogenization. The targeting potential of surface-modified BCL-NS with mean particles size of about 250nm was assessed by in vitro protein adsorption studies using two-dimensional polyacrylamide gel electrophoresis (2-D PAGE), and further evaluated in vivo pharmacokinetics...
December 1, 2017: Colloids and Surfaces. B, Biointerfaces
https://www.readbyqxmd.com/read/28452126/plasma-proteome-of-buffaloes
#14
Leticia Gomes de Pontes, Nayara Rodrigues Vieira Cavassan, Luciana Curtolo de Barros, Rui Seabra Ferreira Junior, Benedito Barraviera, Lucilene Delazari Dos Santos
The proteomic approach has aroused the interest of veterinary medicine researchers, especially regarding the production of biopharmaceuticals and diagnosis of diseases in farm animals. Water buffaloes have gained prominence in the world economy due to the quality of their milk, meat, and leather, in addition to being an important donor of blood components. This work aimed to identify and characterize the proteins present in the blood plasma of Murrah buffaloes (Bubalus bubalis) through 2D electrophoresis, in gel protein digestion followed by mass spectrometry technique and for albumin depletion, in solution protein digestion followed by shotgun analysis...
September 2017: Proteomics. Clinical Applications
https://www.readbyqxmd.com/read/28300160/comparison-of-proteomic-profiles-in-the-zebrafish-retina-during-experimental-degeneration-and-regeneration
#15
Karen Eastlake, Wendy E Heywood, Dhani Tracey-White, Erika Aquino, Emily Bliss, Gerardo R Vasta, Kevin Mills, Peng T Khaw, Mariya Moosajee, G Astrid Limb
Zebrafish spontaneously regenerate the retina after injury. Although the gene expression profile has been extensively studied in this species during regeneration, this does not reflect protein function. To further understand the regenerative process in the zebrafish, we compared the proteomic profile of the retina during injury and upon regeneration. Using two-dimensional difference gel electrophoresis (2D-DIGE) and label-free quantitative proteomics (quadrupole time of flight LC-MS/MS), we analysed the retina of adult longfin wildtype zebrafish at 0, 3 and 18 days after Ouabain injection...
March 16, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28296337/brief-report-anti-carbamylated-protein-antibodies-in-rheumatoid-arthritis-patients-are-reactive-with-specific-epitopes-of-the-human-fibrinogen-%C3%AE-chain
#16
Jonathan D Jones, B JoNell Hamilton, William F C Rigby
OBJECTIVE: Anti-carbamylated protein (anti-CarP) antibodies are associated with the risk and severity of rheumatoid arthritis (RA) and are primarily directed against fibrinogen. The lack of understanding of anti-CarP antibody reactivity has limited analysis of the immunopathogenic associations in RA. To address this shortcoming, we mapped anti-CarP antibody epitope reactivity in RA patient sera. METHODS: Immunoblotting identified a patient serum sample with specific reactivity to the carbamylated human fibrinogen β-chain...
July 2017: Arthritis & Rheumatology
https://www.readbyqxmd.com/read/28107809/plasma-protein-profiling-for-potential-biomarkers-in-the-early-diagnosis-of-alzheimer-s-disease
#17
Yuki Kitamura, Ryoko Usami, Sahoko Ichihara, Hirotaka Kida, Masayuki Satoh, Hidekazu Tomimoto, Mariko Murata, Shinji Oikawa
OBJECTIVES: Alzheimer's disease (AD) is the most common cause of dementia in elderly persons. Since the pathology of AD develops slowly from a preclinical or early phase into a fully expressed clinical syndrome, at the time of diagnosis the disease has been progressing for many years. To facilitate the early diagnosis of AD, we performed protein profiling of blood in patients with mild AD as defined by the Functional Assessment Staging (FAST) scale. METHODS: Plasma samples from mild AD patients and healthy controls were analyzed using two-dimensional differential gel electrophoresis (2D-DIGE) combined with matrix-assisted laser desorption ionization time-of-flight tandem mass spectrometry (MALDI-TOF/TOF/MS) followed by peptide mass fingerprinting...
March 2017: Neurological Research
https://www.readbyqxmd.com/read/27933517/a-novel-mutation-in-the-fibrinogen-a%C3%AE-chain-gly13arg-fibrinogen-nanning-causes-congenital-dysfibrinogenemia-associated-with-defective-peptide-a-release
#18
Jie Yan, Meiling Luo, Peng Cheng, Lin Liao, Xuelian Deng, Donghong Deng, Faquan Lin
Dysfibrinogenemia is characterized by blood coagulation dysfunction induced by an abnormal molecular structure of fibrinogen. Here, we describe a new case. A 32-year-old female was suspected of having dysfibrinogenemia during routine laboratory screening, based on her decreased functional fibrinogen level, normal fibrinogen antigen level, and prolonged thrombin time. We extracted DNA and performed polymerase chain reaction and DNA sequencing to identify genetic mutation. Fibrin polymerization, the kinetics of the fibrinopeptide release, scanning electron microscopy, mass spectrometric analysis, fibrin cross-linking, sodium dodecyl sulfate polyacrylamide gel electrophoresis and western blot were conducted...
April 2017: International Journal of Hematology
https://www.readbyqxmd.com/read/27777179/thiol-oxidation-and-di-tyrosine-formation-in-human-plasma-proteins-induced-by-inflammatory-concentrations-of-hypochlorous-acid
#19
Graziano Colombo, Marco Clerici, Alessandra Altomare, Francesco Rusconi, Daniela Giustarini, Nicola Portinaro, Maria Lisa Garavaglia, Ranieri Rossi, Isabella Dalle-Donne, Aldo Milzani
In this study, we assessed the oxidative damage occurring in plasma proteins when human blood was exposed to inflammatory concentrations of hypochlorous acid (HOCl). We used specific thiol labelling and Western blot analyses to determine protein thiol oxidation, as well as analytical gel filtration HPLC coupled to fluorescence detection to explore formation of high molecular weight (HMW) protein aggregates. Thiol-containing proteins oxidized by HOCl were identified by redox proteomics. Mass spectrometry (MS) analysis was performed to elucidate the protein composition of HMW aggregates...
January 30, 2017: Journal of Proteomics
https://www.readbyqxmd.com/read/27716559/patients-with-calcific-aortic-stenosis-exhibit-systemic-molecular-evidence-of-ischemia-enhanced-coagulation-oxidative-stress-and-impaired-cholesterol-transport
#20
Laura Mourino-Alvarez, Montserrat Baldan-Martin, Laura Gonzalez-Calero, Carlos Martinez-Laborde, Tamara Sastre-Oliva, Rafael Moreno-Luna, Luis F Lopez-Almodovar, Pedro L Sanchez, Francisco Fernandez-Aviles, Fernando Vivanco, Luis R Padial, Finn Akerstrom, Gloria Alvarez-Llamas, Fernando de la Cuesta, María G Barderas
BACKGROUND: The most common valve diseases are calcific aortic stenosis (AS) and aortic regurgitation (AR). The former is characterized by thickening of valve leaflets followed by progressive calcification, which produces progressive aortic valve (AV) narrowing, increased pressure afterload on the left ventricle (LV) and subsequent LV hypertrophy. On the other hand, AR is due to malcoaptation of the valve leaflets with resultant diastolic reflux of blood from aorta back to the LV producing volume and pressure overload and progressive LV dilatation...
December 15, 2016: International Journal of Cardiology
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