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Xiangkai Zhen, Bowen Li, Fen Hu, Shufeng Yan, Gabriele Meloni, Huiliang Li, Ning Shi
Myelin-gene Regulatory Factor (MyRF) is one of the master transcription factors controlling myelin formation and development in oligodendrocytes which is crucial for the powerful brain functions. The N-terminal of MyRF, which contains a proline-rich region and a DNA binding domain (DBD), is auto-cleaved from the ER membrane, and then enters the nucleus to participate in transcription regulation of the myelin genes. Here we report the crystal structure of MyRF DBD. It shows an Ig-fold like architecture which consists of two antiparallel β-sheets with 7 main strands, packing against each other, forming a β-sandwich...
June 16, 2017: Scientific Reports
Kyong-Hoon Choi, Ki Chang Nam, Sang-Yoon Lee, Guangsup Cho, Jin-Seung Jung, Ho-Joong Kim, Bong Joo Park
We report a novel zinc oxide (ZnO) nanoparticle with antioxidant properties, prepared by immobilizing the antioxidant 3-(3,4-dihydroxyphenyl)-2-propenoic acid (caffeic acid, CA) on the surfaces of micro-dielectric barrier discharge (DBD) plasma-treated ZnO nanoparticles. The microstructure and physical properties of ZnO@CA nanoparticles were characterized by field emission scanning electron microscopy (FESEM), transmission electron microscopy (TEM), infrared spectroscopy, and steady state spectroscopic methods...
June 16, 2017: Nanomaterials
A R M da Silva, M L Farias, D L S da Silva, J O Vitoriano, R C de Sousa, C Alves-Junior
In this study, we analyzed seed wettability as well as imbibition and germination after treatment with atmospheric pressure cold plasma (APCP) using dielectric barrier discharge (DBD) in seeds that have very low germination rates. To aid industrial applications, several seeds were simultaneously treated with plasma within a space between two coaxial glass tubes sandwiched by two metal mesh screens that produced high-voltage pulses at 17.5kV with a frequency of 990Hz. Three treatment times (3min, 9min and 15min) as well as untreated seeds were used to conduct the wettability, imbibition and germination tests...
June 1, 2017: Colloids and Surfaces. B, Biointerfaces
Laura J Norton, Samantha Hallal, Elizabeth S Stout, Alister P W Funnell, Richard C M Pearson, Merlin Crossley, Kate G R Quinlan
The Krüppel-like factor (KLF) family of transcription factors play critical roles in haematopoiesis. KLF1, the founding member of the family, has been implicated in the control of both erythropoiesis and megakaryopoiesis. Here we describe a novel system using an artificial dominant negative isoform of KLF1 to investigate the role of KLF1 in the erythroid/megakaryocytic switch in vivo. We developed murine cell lines stably overexpressing a GST-KLF1 DNA binding domain fusion protein (GST-KLF1 DBD), as well as lines expressing GST only as a control...
June 9, 2017: Scientific Reports
Jason L Malaney, John R Demboski, Joseph A Cook
Delimiting species can be challenging, but is a key step for the critical examination of evolutionary history and for prioritizing conservation efforts. Because systematic relationships are often determined iteratively using tests based on taxonomy, such methods can fail to detect cryptic variation and result in biased conclusions. Conversely, discovery-based approaches provide a powerful way to define operational taxonomic units and test species boundaries. We compare both approaches (taxonomy-based delimitation - TBD and discovery-based delimitation - DBD) within North American jumping mice (Zapodinae) using broad sampling, multilocus analyses, and ecological tests...
June 6, 2017: Molecular Phylogenetics and Evolution
E Fadda, M G Nixon
The p53 tumour suppressor is a transcription activator that signals for cell cycle arrest and apoptosis. In its active form p53 is a tetramer, with each monomer organised in domains with different degrees of structural stability, ranging from the well folded DNA-binding domain (DBD) and tetramerization domain (TET), to the intrinsically disordered transactivation domain (TAD), and extreme C-terminal domain (CTD). Compared to all other domains, the structure/function relationship of the p53-CTD within the full-length p53 tetramer is still poorly understood due to its high degree of conformational disorder...
June 9, 2017: Physical Chemistry Chemical Physics: PCCP
Elias J Fernandez, Vandna Gahlot, Celeste Rodriguez, Jacob Amburn
The A/B domains of nuclear receptors such as thyroid receptor α (TRα) are considered to be conformationally flexible and can potentially adopt multiple structural conformations. We used intrinsic tryptophan fluorescence quenching and circular dichroism spectroscopy to characterize the unfolding of this A/B domain upon DNA binding to the contiguous DNA-binding domain (DBD). We propose that this allosteric change in A/B domain conformation can allow it to make the multiple interactions with distinct molecular factors of the transcriptional preinitiation complex...
June 2017: FEBS Open Bio
Teng Guo, Xubing Du, Zhen Peng, Li Xu, Junguo Dong, Jianquan Li, Ping Cheng, Zhen Zhou
RATIONALE: Non-thermal plasma (NTP) has proven to be an effective approach to volatile organic compounds (VOCs) removal. However, harmful organic by-products, produced during NTP-mediated removal of VOCs, hinder practical applications of this technology. It is necessary to characterize the organic by-products to assess their health risks. METHODS: A method is proposed for analyzing and evaluating organic by-products for NTP-mediated removal of VOCs in this work...
June 6, 2017: Rapid Communications in Mass Spectrometry: RCM
Karin M Vermeulen, Daniëlle E M C Jansen, Erik Buskens, Erik J Knorth, Sijmen A Reijneveld
BACKGROUND: In child and youth care, quantitative estimates of the impact of serious behaviour problems have not yet been made. Such input is needed to support decision making on investments in treatment. The aim of this paper was to elicit valuations of social and conduct disorders in children and adolescents from three different perspectives: professionals, youth, and parents. METHODS: We obtained valuations from 25 youth care professionals, 50 children (age 9-10) without serious behaviour problems and 36 adolescents (age 16-17) with and without serious behaviour disorders, and 46 parents with children in the aforementioned age categories...
June 2, 2017: BMC Psychiatry
Mini Tandon, Andrea Giedinghagen
Disruptive behavior disorders (DBDs), specifically oppositional defiant disorder and conduct disorder, are common, serious, and treatable conditions among preschoolers. DBDs are marked by frequent aggression, deceitfulness, and defiance, and often persist through the lifespan. Exposure to harsh or inconsistent parenting, as frequently seen with parental depression and stress, increases DBD risk. Candidate genes that may increase DBD risk in the presence of childhood adversity have also been identified, but more research is needed...
July 2017: Child and Adolescent Psychiatric Clinics of North America
Chien-Cheng Chen, Alina P Montalbano, Imran Hussain, Wan-Ru Lee, Carole R Mendelson
The mechanisms whereby progesterone (P4) acting via progesterone receptor (PR) inhibits proinflammatory/contractile gene expression during pregnancy are incompletely defined. Using immortalized human myometrial (hTERT-HM) cells stably expressing wild-type PR-A or PR-B (PRWT), we found that P4 significantly inhibited IL-1β induction of the NF-κB target genes, COX-2 and IL-8 P4/PRWT transrepression occurred at the level of transcription initiation and was mediated by decreased recruitment of NF-κB p65 and RNA Pol II to COX-2 and IL-8 promoters...
June 2, 2017: Journal of Biological Chemistry
Apurva Patange, Daniela Boehm, Carmen Bueno-Ferrer, P J Cullen, Paula Bourke
Brochothrix thermosphacta is the predominant spoilage microorganism in meat and its control in processing environments is important to maintain meat product quality. Atmospheric cold plasma is of interest for control of pathogenic and spoilage microorganisms in foods. This study ascertained the potential of dielectric barrier discharge atmospheric cold plasma (DBD-ACP) for control of B. thermosphacta, taking microbial and food environment factors into consideration, and investigated the shelf-life of lamb chop after in-package plasma treatment in modified atmosphere...
September 2017: Food Microbiology
Ya-Jie Zhang, Yi Cai, Yong-Liang Yu, Jian-Hua Wang
A miniature optical emission spectrometric (OES) system is developed by incorporating dielectric barrier discharge (DBD) microplasma as excitation source in a lab-on-valve (LOV) configuration for trace cadmium analysis. The entire system integrates bead injection (BI) sample pretreatment, post-column derivatization, in-situ nebulization and OES detection. Trace cadmium in sample solution is separated and preconcentrated by flowing through a 2-(5-Br-2-pyridylazo)-5-dethylaminophenol (5-Br-PADAP) loaded microcolumn in LOV, followed by elution with 20 μL of 0...
July 11, 2017: Analytica Chimica Acta
Russell Hodgson, Alastair L Young, Magdy A Attia, J Peter A Lodge
Organ transplantation is the most successful treatment for some forms of organ failure yet a lack of organs means many die on the waiting list. In the UK, the Organ Donation Taskforce was set up to identify barriers to organ donation and in 2008 released its first report (ODTR). This study assesses the success since the ODTR and examines the impact of the UK's controlled Donation after Circulatory Death (DCD) programme and the controversies surrounding it. There were 12864 intended Donation after Brain Death (DBD) or DCD donors from April 2004 to March 2014...
May 28, 2017: American Journal of Transplantation
Timothy J Cole, Morag J Young
The mineralocorticoid receptor (MR) mediates the actions of two important adrenal corticosteroid hormones, aldosterone and cortisol. The cell signalling roles of the MR in vivo have expanded enormously since the cloning of human MR gene 30 years ago and the first MR gene knockout in mice nearly 20 years ago. Complete ablation of the MR revealed important roles postnatally for regulation of kidney epithelial functions, with MR-null mice dying 1-2 weeks postnatally from renal salt wasting and hyperkalaemia, with elevated plasma renin and aldosterone...
July 2017: Journal of Endocrinology
Xiao-Dan Ren, Ying-Wen Zhang, Xiu-Ping Wang, Ya-Rong Li
BACKGROUND: Dysfunction of glomerular mesangial cells (GMCs) plays an important role in pathogenesis of diabetic nephropathy. Here, we investigated the effects of Dangguibuxue decoction (DBD), an herbal traditional Chinese medicinal (TCM) formula composed of Astragali Radix and Angelicae Sinensis Radix, on GMC proliferation and fibrogenesis under high-glucose (HG) conditions. METHODS: Sixty male Sprague Dawley rats were divided into 5 groups and administered intragastric 0...
May 25, 2017: BMC Complementary and Alternative Medicine
L Shriki-Tal, H Avrahamy, Y Pollak, V Gross-Tsur, L Genstil, H J Hirsch, F Benarroch
BACKGROUND: Psychiatric manifestations in Prader-Willi Syndrome (PWS) are common and often are the most debilitating problem in these individuals. We present an epidemiological nation-wide survey of psychiatric diagnoses in the PWS population, based on full-range psychiatric interviews. METHODS: We studied the distribution of psychiatric diagnoses (as opposed to a symptom-based approach) in the Israel national cohort of adolescents and adults with PWS. There was a total of 53 (32 males) ages 12 years and older...
April 5, 2017: European Psychiatry: the Journal of the Association of European Psychiatrists
Ling Li, Ning Li, Chongxiang He, Wei Huang, Xiaoli Fan, Zibiao Zhong, Yanfeng Wang, Qifa Ye
A large number of previous clinical studies have reported a delayed graft function for brain dead donors, when compared with living relatives or cadaveric organ transplantations. However, there is no accurate method for the quality evaluation of kidneys from brain‑dead donors. In the present study, two‑dimensional gel electrophoresis and MALDI‑TOF MS‑based comparative proteomic analysis were conducted to profile the differentially‑expressed proteins between brain death and the control group renal tissues...
July 2017: Molecular Medicine Reports
Shanping Li, Xiaoping Wang, Lijun Liu, Yongbo Guo, Qinglin Mu, Abdelwahid Mellouki
A synergistic system of dielectric barrier discharge (DBD) combined with La/Ce-TiO2 was developed to investigate the decomposition performance of the environmentally persistent perfluorooctanoic acid (PFOA). The La/Ce-TiO2 was modified by sol-gel method and characterized by XRD, SEM, and energy dispersive X-ray. The effects of PFOA concentration, applied voltage, initial pH, liquid conductivity, and additives on the removal rate of PFOA were explored. The results showed that the La/Ce-TiO2 exhibited excellent catalytic effects on PFOA degradation in DBD system...
May 20, 2017: Environmental Science and Pollution Research International
Xing Liu, Zhenli Zhu, Huilai Li, Dong He, Yatai Li, Hongtao Zheng, Yiqun Gan, Yanxiang Li, Nicholas Stanley Belshaw, Shenghong Hu
In the present study, a novel and sensitive liquid spray dielectric barrier discharge induced plasma-chemical vapor generation technique (LSDBD-CVG) is developed for the determination of lead concentration by inductively coupled plasma mass spectrometry (ICPMS). The dissolved Pb(2+) is readily converted to volatile species by LSDBD plasma induced chemical processes in the presence of 5% (v/v) formic acid in a supporting electrolyte (HCl, 0.01 mol L(-1)). In this LSDBD approach, the sample solution is converted to aerosol and simultaneously mixed with the DBD plasma generated at the nozzle of a pneumatic nebulizer, which greatly facilitates Pb vapor generation because of the enhanced interaction of sprayed analytes and the plasma...
May 25, 2017: Analytical Chemistry
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