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https://www.readbyqxmd.com/read/28701406/structure-and-functional-basis-for-an-ebna1-hexameric-ring-in-epstein-barr-virus-episome-maintenance
#1
Julianna S Deakyne, Kimberly Malecka, Troy E Messick, Paul M Lieberman
Epstein-Barr virus (EBV) establishes a stable latent infection that can persist for the life of the host. EBNA1 is required for the replication, maintenance, and segregation of the latent episome but the structural features of EBNA1 that confer each of these functions are not completely understood. Here, we have solved the x-ray crystal structure of an EBNA1 DNA binding domain (DBD) and discovered a novel hexameric ring oligomeric form. The oligomeric interface pivoted around residue T585 as a joint that links and stabilizes higher order EBNA1 complexes...
July 12, 2017: Journal of Virology
https://www.readbyqxmd.com/read/28695847/dna-binding-domain-of-myelin-gene-regulatory-factor-purification-crystallization-and-x-ray-analysis
#2
WenYu Wu, Xiangkai Zhen, Ning Shi
The myelin sheath, which envelops axons in the vertebrate central nervous system, is crucial for the rapid conduction of action potentials. Myelin-gene regulatory factor (MRF) is a recently identified transcription factor that is required for myelin-sheath formation. Loss of MRF leads to demyelinating diseases and motor learning deficiency. MRF is a membrane-bound transcription factor that undergoes autocleavage from the endoplasmic reticulum membrane. The N-terminus of MRF contains a DNA-binding domain (DBD) that functions as a homotrimer...
July 1, 2017: Acta Crystallographica. Section F, Structural Biology Communications
https://www.readbyqxmd.com/read/28690174/clinical-outcomes-of-donation-after-circulatory-death-liver-transplantation-in-primary-sclerosing-cholangitis
#3
Palak J Trivedi, Irene Scalera, Emma Slaney, Richard W Laing, Bridget Gunson, Gideon M Hirschfield, Andrea Schlegel, James Ferguson, Paolo Muiesan
BACKGROUND AND AIM: Primary sclerosing cholangitis (PSC) is a progressive fibro-inflammatory cholangiopathy for which liver transplantation is the only life-extending intervention. These patients may benefit from accepting liver donation after circulatory death (DCD), however their subsequent outcome is unknown. METHODS: Clinical outcomes were prospectively evaluated in PSC patients undergoing transplantation from 2006 to 2016 stratified by donor type (DCD, n=35 vs...
July 6, 2017: Journal of Hepatology
https://www.readbyqxmd.com/read/28688125/novel-ex-vivo-training-model-for-free-hand-insertion-using-a-double-bending-peroral-direct-cholangioscope
#4
Sakiko Tsukamoto Naito, Takao Itoi, Kenjiro Yamamoto, Takayoshi Tsuchiya, Shujiro Tsuji, Reina Tanaka, Mitsuyoshi Honjo, Shuntaro Mukai, Yukitoshi Matsunami, Yasutsugu Asai, Yuichi Nagakawa, Nobuhito Ikeuchi, Atsushi Sofuni
BACKGROUND AND AIM: Several experts of direct peroral videocholangioscopy (D-PVCS) using a conventional ultraslim endoscope have reported its usefulness for the diagnosis and therapy of biliary tract diseases. We have additionally developed a dedicated double-bending D-PVCS (DBD-PVCS) technique for free-hand scope insertion. In this study, we developed an ex-vivo training model for the free-hand DBD-PVCS technique and compared it with the technique using a conventional ultraslim endoscope...
July 7, 2017: Journal of Gastroenterology and Hepatology
https://www.readbyqxmd.com/read/28686912/antiproliferative-and-apoptotic-activities-of-sequence-specific-histone-acetyltransferase-inhibitors
#5
Zutao Yu, Junichi Taniguchi, Yulei Wei, Ganesh N Pandian, Kaori Hashiya, Toshikazu Bando, Hiroshi Sugiyama
In parallel to monomeric epigenetic regulators, sequence-specific epigenetic regulators represent versatile synthetic dual-target ligands that achieve regulatory control over multi-gene networks. Development of DNA-binding domain (DBD)-HDAC inhibitors and DBD-HAT activators, which result in increased histone acetylation, has become one promising research field. However, there is no report regarding the gene regulatory pattern by sequence-specific epigenetic repressor. We report here for the first time, the synthesis of DBD-HAT inhibitors and demonstrate that these conjugates could retain their dual-target activity using predicted working model of thermal stability assay and in vitro HAT activity assay...
June 23, 2017: European Journal of Medicinal Chemistry
https://www.readbyqxmd.com/read/28683139/genome-wide-survey-of-heat-shock-factors-and-heat-shock-protein-70s-and-their-regulatory-network-under-abiotic-stresses-in-brachypodium-distachyon
#6
Feng Wen, Xiaozhu Wu, Tongjian Li, Mingliang Jia, Xinshen Liu, Peng Li, Xiaojian Zhou, Xinxin Ji, Xiaomin Yue
The heat shock protein 70s (Hsp70s) and heat shock factors (Hsfs) play key roles in protecting plant cells or tissues from various abiotic stresses. Brachypodium distachyon, recently developed an excellent model organism for functional genomics research, is related to the major cereal grain species. Although B. distachyon genome has been fully sequenced, the information of Hsf and Hsp70 genes and especially the regulatory network between Hsfs and Hsp70s remains incomplete. Here, a total of 24 BdHsfs and 29 BdHsp70s were identified in the genome by bioinformatics analysis and the regulatory network between Hsfs and Hsp70s were performed in this study...
2017: PloS One
https://www.readbyqxmd.com/read/28678405/life-after-death-breathing-life-into-lung-transplantation-from-donation-after-circulatory-death-donors
#7
EDITORIAL
S J Bozso, J Nagendran
As there is a growing population of potential recipients that may benefit from lung transplantation, there remains a profound deficiency in donor lungs suitable for transplantation. An area of potential donor pool expansion exists in donation after circulatory death (DCD) donors. Lung transplantation utilizing DCD donors continues to be disproportionately less than donation after brain death (DBD) donors at many high-volume centers. This article is protected by copyright. All rights reserved.
July 5, 2017: American Journal of Transplantation
https://www.readbyqxmd.com/read/28678058/donors-after-circulatory-death-pancreas-transplantation
#8
Shruti Mittal, James Gilbert, Peter J Friend
PURPOSE OF REVIEW: The use of organs from donors after circulatory death (DCD) has become standard practice in solid organ transplantation of most abdominal organs and has been used successfully in some centres for pancreas transplantation. Nevertheless, concerns regarding poor graft outcomes and complications remain. This review aims to discuss the current state of DCD pancreas transplantation and the associated outcomes. RECENT FINDINGS: In many countries, whereas the number of donors after brainstem death (DBD) remains stable, the mean age and BMI have increased making these donors, previously considered to be low risk, now more marginal...
August 2017: Current Opinion in Organ Transplantation
https://www.readbyqxmd.com/read/28676789/importance-of-the-sequence-directed-dna-shape-for-specific-binding-site-recognition-by-the-estrogen-related-receptor
#9
Kareem Mohideen-Abdul, Karima Tazibt, Maxime Bourguet, Isabelle Hazemann, Isabelle Lebars, Maria Takacs, Sarah Cianférani, Bruno P Klaholz, Dino Moras, Isabelle M L Billas
Most nuclear receptors (NRs) bind DNA as dimers, either as hetero- or as homodimers on DNA sequences organized as two half-sites with specific orientation and spacing. The dimerization of NRs on their cognate response elements (REs) involves specific protein-DNA and protein-protein interactions. The estrogen-related receptor (ERR) belongs to the steroid hormone nuclear receptor (SHR) family and shares strong similarity in its DNA-binding domain (DBD) with that of the estrogen receptor (ER). In vitro, ERR binds with high affinity inverted repeat REs with a 3-bps spacing (IR3), but in vivo, it preferentially binds to single half-site REs extended at the 5'-end by 3 bp [estrogen-related response element (ERREs)], thus explaining why ERR was often inferred as a purely monomeric receptor...
2017: Frontiers in Endocrinology
https://www.readbyqxmd.com/read/28667961/novel-design-of-high-voltage-pulse-source-for-efficient-dielectric-barrier-discharge-generation-by-using-silicon-diodes-for-alternating-current
#10
Hoa Thi Truong, Misaki Hayashi, Yoshihiko Uesugi, Yasunori Tanaka, Tatsuo Ishijima
This work focuses on design, construction, and optimization of configuration of a novel high voltage pulse power source for large-scale dielectric barrier discharge (DBD) generation. The pulses were generated by using the high-speed switching characteristic of an inexpensive device called silicon diodes for alternating current and the self-terminated characteristic of DBD. The operation started to be powered by a primary DC low voltage power supply flexibly equipped with a commercial DC power supply, or a battery, or DC output of an independent photovoltaic system without transformer employment...
June 2017: Review of Scientific Instruments
https://www.readbyqxmd.com/read/28667662/synthesis-and-evaluation-of-novel-thiazole-based-derivatives-as-selective-inhibitors-of-dna-binding-domain-of-the-androgen-receptor
#11
Ruixue Xu, Ye Tian, Shenzhen Huang, Jiang Yu, Yufang Deng, Miao Zhan, Tao Zhang, Fangying Wang, Lifeng Zhao, Yuanwei Chen
A series of thiazole-based inhibitors selectively targeting DNA-binding domain of the androgen receptor (AR) were synthesized, evaluated and the SAR data were summarized. We identified a novel compound SKLB-C2807 that effectively inhibited the human prostate cancer cell line LNCaP/AR with the IC50 value of 0.38 μM without significant antiproliferative effects on other cell lines PC-3 (AR-negative), SW620, MCF-7 (ER-positive) and L-O2 (non-cancerous). This compound also considerably decreased the expression of prostate-specific antigen (PSA)...
July 1, 2017: Chemical Biology & Drug Design
https://www.readbyqxmd.com/read/28663896/evaluation-of-non-thermal-plasma-induced-anticancer-effects-on-human-colon-cancer-cells
#12
Jae-Sun Choi, Jeongho Kim, Young-Jun Hong, Woom-Yee Bae, Eun Ha Choi, Joo-Won Jeong, Hun-Kuk Park
Non-thermal atmospheric-pressure plasma has been introduced in various applications such as sterilization, wound healing, blood coagulation, and other biomedical applications. The most attractive application of non-thermal atmospheric-pressure plasma is in cancer treatment, where the plasma is used to produce reactive oxygen species (ROS) to facilitate cell apoptosis. We investigate the effects of different durations of exposure to dielectric-barrier discharge (DBD) plasma on colon cancer cells using measurement of cell viability and ROS levels, western blot, immunocytochemistry, and Raman spectroscopy...
May 1, 2017: Biomedical Optics Express
https://www.readbyqxmd.com/read/28659017/effect-of-tio2-gac-and-water-vapor-on-chloroform-decomposition-in-a-hybrid-plasma-catalytic-system
#13
Abedi Kamal Ad-Din, Ghorbani-Shahna Farshid, Bahrami Abdulrahman, Ebrahimi Hossein, Maleki Afshin, Madjidi Faramarz, Musavi Saeed, Mohammadi Ebrahim, Giahi Omid
This paper presents the combination of TiO2/GAC catalyst and NTP for the decomposition of chloroform using a DBD reactor. The experiments were performed using an AC transformer as the power supply system to determine the optimal conditions of the chloroform conversion in the presence of a hydrogen-rich substance i.e. water vapor. TiO2/GAC enhanced the removal efficiency and also CO2 selectivity significantly, leading to an acceptable conversion rate at SIEs higher than 400 J L(-1). The adsorption property of GAC was noticed to be an effective factor for catalytic activity by increasing the residence time, although the higher retention time prevented the accurate determination of chlorine and carbon balance...
June 29, 2017: Environmental Technology
https://www.readbyqxmd.com/read/28655070/inhibitory-effect-of-carnosol-on-phthalic-anhydride-induced-atopic-dermatitis-via-inhibition-of-stat3
#14
Do Yeon Lee, Chul Ju Hwang, Ji Yeon Choi, Mi Hee Park, Min Ji Song, Ki Wan Oh, Dong Ju Son, Seung Hwa Lee, Sang Bae Han, Jin Tae Hong
Carnosol is a phenolic antioxidant present in rosemary (Rosmarinus officinalis). It is known for anti-inflammatory effects, analgesic activity and anti-cancer effects. However, no study has been dedicated yet to its effect on atopic dermatitis (AD). Here, we show that carnosol effectively inhibited LPS-induced nitric oxide (NO) generation and expression of inflammatory marker proteins (iNOS and COX-2) in RAW 264.7 cells. In addition, carnosol effectively inhibits the phosphorylation of STAT3 and DNA binding activity in RAW 264...
June 27, 2017: Biomolecules & Therapeutics
https://www.readbyqxmd.com/read/28644006/structure-of-the-forkhead-domain-of-foxa2-bound-to-a-complete-dna-consensus-site
#15
Jun Li, Ana Carolina Dantas Machado, Ming Guo, Jared M Sagendorf, Zhan Zhou, Longying Jiang, Xiaojuan Chen, Daichao Wu, Lingzhi Qu, Zhuchu Chen, Lin Chen, Remo Rohs, Yongheng Chen
FOXA2, a member of the forkhead family of transcription factors, plays essential roles in liver development and bile acid homeostasis. In this study, we report a 2.8 Å co-crystal structure of the FOXA2 DNA-binding domain (FOXA2-DBD) bound to a DNA duplex containing a forkhead consensus binding site (GTAAACA). The FOXA2-DBD adopts the canonical winged-helix fold, with helix H3 and wing 1 regions mainly mediating the DNA recognition. Although the wing 2 region was not defined in the structure, isothermal titration calorimetry assays suggested that this region was required for optimal DNA binding...
July 11, 2017: Biochemistry
https://www.readbyqxmd.com/read/28640537/changes-in-the-biomechanical-properties-of-a-single-cell-induced-by-nonthermal-atmospheric-pressure-micro-dielectric-barrier-discharge-plasma
#16
Hyeongwon Choi, Eun Ha Choi, Kyung Sook Kim
Mechanical properties of a single cell are closely related to the fate and functions of the cell. Changes in mechanical properties may cause diseases or cell apoptosis. Selective cytotoxic effects of nonthermal atmospheric pressure micro-dielectric barrier discharge (DBD) plasma have been demonstrated on cancer cells. In this work, changes in the mechanical properties of a single cell induced by nonthermal atmospheric pressure micro-DBD plasma were investigated using atomic force microscopy (AFM). Two cervical cancer cell lines (HeLa and SiHa) and normal human fibroblast cells (HFBs) were exposed to micro-DBD plasma for various exposure times...
June 22, 2017: Microscopy Research and Technique
https://www.readbyqxmd.com/read/28640070/early-outcomes-of-the-new-uk-deceased-donor-kidney-fast-track-offering-scheme
#17
Chris J Callaghan, Lisa Mumford, Laura Pankhurst, Richard J Baker, J Andrew Bradley, Christopher J E Watson
BACKGROUND: The UK Kidney Fast-Track Scheme (KFTS) was introduced in 2012 to identify kidneys at high risk of discard and to rapidly facilitate transplantation. A retrospective analysis of kidneys transplanted through the KFTS was undertaken. METHODS: UK Transplant Registry data were collected on deceased donor kidneys implanted between 1 November 2012 and 30 April 2015 (DBD donors) and 1 March 2013 and 30 April 2015 (DCD donors). Posttransplant outcomes included 1-year eGFR and death-censored graft survival (DCGS)...
June 21, 2017: Transplantation
https://www.readbyqxmd.com/read/28639260/improving-thermostability-of-r-selective-amine-transaminase-from-aspergillus-terreus-through-introduction-of-disulfide-bonds
#18
Dong-Fang Xie, Hui Fang, Jia-Qi Mei, Jin-Yan Gong, Hong-Peng Wang, Xiu-Ying Shen, Jun Huang, Le-He Mei
To improve the thermostability of (R)-selective amine transaminase from Aspergillus terreus (AT-ATA), we used computer software Disulfide by Design and Modelling of Disulfide Bonds in Proteins to identify mutation sites where the disulfide bonds were most likely to form. We obtained three stabilized mutants (N25C-A28C, R131C-D134C, M150C-M280C) from seven candidates by site-directed mutagenesis. Compared to the wild type, the best two mutants N25C-A28C and M150C-M280C showed improved thermal stability with a 3...
June 21, 2017: Biotechnology and Applied Biochemistry
https://www.readbyqxmd.com/read/28623291/crystal-structure-of-the-dna-binding-domain-of-myelin-gene-regulatory-factor
#19
Xiangkai Zhen, Bowen Li, Fen Hu, Shufeng Yan, Gabriele Meloni, Huiliang Li, Ning Shi
Myelin-gene Regulatory Factor (MyRF) is one of the master transcription factors controlling myelin formation and development in oligodendrocytes which is crucial for the powerful brain functions. The N-terminal of MyRF, which contains a proline-rich region and a DNA binding domain (DBD), is auto-cleaved from the ER membrane, and then enters the nucleus to participate in transcription regulation of the myelin genes. Here we report the crystal structure of MyRF DBD. It shows an Ig-fold like architecture which consists of two antiparallel β-sheets with 7 main strands, packing against each other, forming a β-sandwich...
June 16, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28621707/antioxidant-potential-and-antibacterial-efficiency-of-caffeic-acid-functionalized-zno-nanoparticles
#20
Kyong-Hoon Choi, Ki Chang Nam, Sang-Yoon Lee, Guangsup Cho, Jin-Seung Jung, Ho-Joong Kim, Bong Joo Park
We report a novel zinc oxide (ZnO) nanoparticle with antioxidant properties, prepared by immobilizing the antioxidant 3-(3,4-dihydroxyphenyl)-2-propenoic acid (caffeic acid, CA) on the surfaces of micro-dielectric barrier discharge (DBD) plasma-treated ZnO nanoparticles. The microstructure and physical properties of ZnO@CA nanoparticles were characterized by field emission scanning electron microscopy (FESEM), transmission electron microscopy (TEM), infrared spectroscopy, and steady state spectroscopic methods...
June 16, 2017: Nanomaterials
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