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Huiwen Wu, Jashil Hyun, Maria A Martinez-Yamout, Sung Jean Park, Jane Dyson
The cancer-suppressing transcription factor p53 is regulated by a wide variety of cellular factors, including many chaperones. The DNA-binding domain (DBD) of p53 is known to interact with the chaperone Hsp90, but the role of other members of the chaperone network, including co-chaperones such as p23 is unknown. Using a combination of NMR titration, isothermal titration calorimetry, fluorescence anisotropy and native agarose gel electrophoresis, we have identified a direct interaction between the p53 DBD and the Hsp90 co-chaperone p23 that occurs in the absence of Hsp90...
January 15, 2018: Biochemistry
Olawunmi Olagundoye, Alex Igundunasse, Morenike Aluko
Background Despite the need to curb the menace resulting from the negative trajectory of disruptive behaviour disorders (DBD) in societies of the world today, there is yet a dearth of locally standardised tools for the early detection of these disorders in Nigeria. This study was aimed at standardising the DBD teacher rating scale (DBD-TRS) to be culturally specific using teachers' ratings of their students. Objectives To establish norm scores for the three categories of DBD on the DBD-TRS, to evaluate the reliability, validity, predictive power, sensitivity and specificity of DBD-TRS items for identifying DBD symptoms amongst children/adolescents between the ages of 4 and 16 years...
January 13, 2018: International Journal of Adolescent Medicine and Health
Dingqiang Fang, Armelle Lengronne, Di Shi, Romain Forey, Magdalena Skrzypczak, Krzysztof Ginalski, Changhui Yan, Xiaoke Wang, Qinhong Cao, Philippe Pasero, Huiqiang Lou
Initiation of eukaryotic chromosome replication follows a spatiotemporal program. The current model suggests that replication origins compete for a limited pool of initiation factors. However, it remains to be answered how these limiting factors are preferentially recruited to early origins. Here, we report that Dbf4 is enriched at early origins through its interaction with forkhead transcription factors Fkh1 and Fkh2. This interaction is mediated by the Dbf4 C terminus and was successfully reconstituted in vitro...
January 12, 2018: Genes & Development
Tiffany J Zens, Juan S Danobeitia, Glen Leverson, Peter J Chlebeck, Laura J Zitur, Robert R Redfield, Anthony M D'Alessandro, Scott Odorico, Dixon B Kaufman, Luis A Fernandez
INTRODUCTION: Renal transplant outcomes result from a combination of factors. Traditionally, donor factors were summarized by classifying kidneys as extended criteria or standard criteria. In 2014, the nomenclature changed to describe donor factors with the kidney donor profile index (KDPI). We aim to evaluate the relationship between KDPI and delayed graft function, and the impact KDPI on transplant outcomes for both donor after cardiac death (DCD) and donor after brain death (DBD) ...
January 3, 2018: Clinical Transplantation
Martha I Dávila-Rodríguez, Elva I Cortés-Gutiérrez, Roberto Hernández-Valdés, Karla Guzmán-Cortés, Rosa E De León-Cantú, Ricardo M Cerda-Flores, Enrique Báez-De la Fuente
The purpose of this study was to evaluate DNA damage in the whole genome of peripheral blood leukocytes from patients with acute myeloid leukemia (AML) compared with a control group using DNA breakage detection-fluorescent in situ hybridization (DBD-FISH). Our results suggest that the DNA damage detected in patients with newly diagnosed AML was similar to that observed for the controls; this might be explained by the stimulation of a repair pathway by the pathogenesis itself. These findings indicate that inhibiting the repair pathway could be proposed to enhance the efficacy of chemotherapy...
December 11, 2017: European Journal of Histochemistry: EJH
Lingai Mao, Zhizong Chen, Xinyue Wu, Xiujuan Tang, Shuiliang Yao, Xuming Zhang, Boqiong Jiang, Jingyi Han, Zuliang Wu, Hao Lu, Tomohiro Nozaki
A dielectric barrier discharge (DBD) catalyst hybrid reactor with CeO2/γ-Al2O3 catalyst balls was investigated for benzene decomposition at atmospheric pressure and 30 °C. At an energy density of 37-40 J/L, benzene decomposition was as high as 92.5% when using the hybrid reactor with 5.0wt%CeO2/γ-Al2O3; while it was 10%-20% when using a normal DBD reactor without a catalyst. Benzene decomposition using the hybrid reactor was almost the same as that using an O3 catalyst reactor with the same CeO2/γ-Al2O3 catalyst, indicating that O3 plays a key role in the benzene decomposition...
December 28, 2017: Journal of Hazardous Materials
Yijing Luo, Yuan Yang, Yao Lin, Yunfei Tian, Li Wu, Lu Yang, Xiandeng Hou, Chengbin Zheng
A new sample digestion method using a double layer and coaxial dielectric barrier discharge (DBD) digestion reactor was developed for the sensitive determination of trace elements in rice samples. All the operation parameters of the DBD mi-croplasma and other digestion conditions were carefully optimized. Three DBD-digestion modes were investigated for real matrix samples, including H2O-DBD-digestion, H2O2-DBD-digestion and HNO3-DBD-digestion systems. Among the three modes, H2O-DBD-digestion system provides a suitable digestion of sample without any additional chemicals, achieving environmental friendly sample treatment and eliminating the potential interferences...
January 5, 2018: Analytical Chemistry
Marcelo Recondo Cheffe, Jorge Diego Valentini, Marcus Vinicius Martins Collares, Pedro Salomão Piccinini, Jefferson Luis Braga da Silva
BACKGROUND: Dynamic breast deformity (DBD) is characterized by visible distortion and deformity of the breast due to contraction of the pectoralis major muscle after submuscular breast augmentation; fortunately, in most cases, this is not a clinically significant complaint from patients. The purpose of this study is to present a simple method for objectively measuring DBD in patients submitted to dual plane breast augmentation (DPBA). METHODS: We studied 32 women, between 18 and 50 years old, who underwent primary DPBA with at least 1 year of follow-up...
January 4, 2018: Aesthetic Plastic Surgery
Zuliang Wu, Zhoubin Zhu, Xiaodong Hao, Weili Zhou, Jingyi Han, Xiujuan Tang, Shuiliang Yao, Xuming Zhang
Non-thermal plasma technology has great potential in reducing polycyclic aromatic hydrocarbons (PAHs) emission. But in plasma-alone process, various undesired by-products are produced, which causes secondary pollutions. Here, a dielectric barrier discharge (DBD) reactor has been developed for the oxidation of naphthalene over a TiO2/diatomite catalyst at low temperature. In comparison to plasma-alone process, the combination of plasma and TiO2/diatomite catalyst significantly enhanced naphthalene conversion (up to 40%) and COx selectivity (up to 92%), and substantially reduced the formation of aerosol (up to 90%) and secondary volatile organic compounds (up to near 100%)...
December 25, 2017: Journal of Hazardous Materials
Kunyu Zheng, Michael J Dolan, Peter J Haferl, Hamid Badiei, Kaveh Jorabchi
Atmospheric-pressure dielectric barrier discharge (AP-DBD) has emerged in recent years as a versatile plasma for molecular ionization and elemental spectroscopy. However, its capabilities as an elemental ion source have been less explored, partly because of difficulties in detection of positive elemental ions from this low-gas-temperature plasma. Here, we investigate detection of negative elemental ions to enable elemental mass spectrometry (MS) using AP-DBD. A gas chromatograph is coupled to a helium AP-DBD and positioned in front of an atmospheric-sampling mass spectrometer with no modifications to the ion sampling interface...
December 20, 2017: Analytical Chemistry
Jae Seung Kang, Seungyeoun Lee, Donghee Son, Youngmin Han, Kyung Bun Lee, Jae Ri Kim, Wooil Kwon, Sun-Whe Kim, Jin-Young Jang
BACKGROUND: The new 8th American Joint Committee on Cancer (AJCC) staging has recently been released and there are major changes in distal bile duct (DBD) cancer staging. However, clinical validation is needed before the changes can be widely implemented. METHODS: This study was performed to evaluate the prognostic predictability of the 8th AJCC staging compared with that of the 7th using C statistics. RESULTS: A total of 293 consecutive patients who had curative-intended surgery were enrolled...
November 3, 2017: Journal of Hepato-biliary-pancreatic Sciences
Cheukfai Li, Qiang Zhao, Wei Zhang, Maogen Chen, Weiqiang Ju, Linwei Wu, Ming Han, Yi Ma, Xiaofeng Zhu, Dongping Wang, Zhiyong Guo, Xiaoshun He
BACKGROUND Poor transplant outcome was observed in donation after brain death followed by circulatory death (DBCD), since the donor organs suffered both cytokine storm of brain death and warm ischemia injury. MicroRNAs (miRNAs) have emerged as promising disease biomarkers, so we sought to establish a miRNA signature of porcine DBCD and verify the findings in human liver transplantation. MATERIAL AND METHODS MiRNA expression was determined with miRNA sequencing in 3 types of the porcine model of organ donation, including donation after brain death (DBD) group, donation after circulatory death (DCD) group, and DBCD group...
December 11, 2017: Medical Science Monitor: International Medical Journal of Experimental and Clinical Research
Carl Fletcher, Richard Sleeman, John Luke, Peter Luke, James W Bradley
The detection of explosives is of great importance, as is the need for sensitive, reliable techniques that require little or no sample preparation and short run times for high throughput analysis. In this work, a novel ionisation source is presented based on a dielectric barrier discharge (DBD). This not only effects desorption and ionisation, but also forms an ionic wind, providing mass transportation of ions towards the mass spectrometer. Furthermore, the design incorporates two asymmetric alumina sheets, each containing three DBDs, so that a large surface area can be analysed...
December 6, 2017: Journal of Mass Spectrometry: JMS
Martí Manyalich, Helen Nelson, Francis L Delmonico
PURPOSE OF REVIEW: The global shortage of organ donors will not be resolved solely by relying on deceased donation following a brain death determination (DBD). Expansion of deceased donation after circulatory death (DCD) will be needed to address the shortfall of organs for transplantation. Approximately 120 000 organ transplants are performed each year; however, the WHO estimates that this number of transplants only resolves 10% of the annual worldwide transplant need. RECENT FINDINGS: The report addresses the opportunity of DCD expansion by evaluating the DCD potential that is not being realized, the utility of DCD enabling DBD to emerge in some clinical situations, by the effectiveness of a donor registry in achieving DCD, and by the current clinical research of heart, lung, and liver transplantation from DCD...
December 4, 2017: Current Opinion in Organ Transplantation
Koji Matsuo, Kenichiro Harada, Yusuke Fujita, Yasumasa Okamoto, Miho Ota, Hisashi Narita, Benson Mwangi, Carlos A Gutierrez, Go Okada, Masahiro Takamura, Hirotaka Yamagata, Ichiro Kusumi, Hiroshi Kunugi, Takeshi Inoue, Jair C Soares, Shigeto Yamawaki, Yoshifumi Watanabe
No neuroanatomical substrates for distinguishing between depression of bipolar disorder (dBD) and major depressive disorder (dMDD) are currently known. The aim of the current multicenter study was to identify neuroanatomical patterns distinct to depressed patients with the two disorders. Further analysis was conducted on an independent sample to enable generalization of results. We directly compared MR images of these subjects using voxel-based morphometry (VBM) and a support vector machine (SVM) algorithm using 1531 participants...
November 30, 2017: Cerebral Cortex
Ali A Ghazi, Azita Zadeh-Vakili, Marjan Zarif Yeganeh, Shahram Alamdari, Atieh Amouzegar, Ali Akbar Khorsandi, Alireza Amirbaigloo, Fereidoun Azizi
Purpose and Methods: Hereditary vitamin D resistant rickets (HVDRR) is a rare disease that presents with signs and symptoms of rickets, alopecia, and growth retardation during the early years of life. The disease is caused by mutations in the vitamin D receptor (VDR) gene, which leads to unresponsiveness of the mutant receptor to 1-25(OH) 2 D3. The disease is transmitted as an autosomal recessive disorder and is found with equal frequency in males and females. The disease is rarely encountered and only about 100 cases are reported so far...
July 2017: International Journal of Endocrinology and Metabolism
Qiao Guo, Ying Wang, Haoran Zhang, Guangzhou Qu, Tiecheng Wang, Qiuhong Sun, Dongli Liang
Atmospheric dielectric barrier discharge (DBD) was attempted to improve the resistance of wheat seed to drought stress. Effects of DBD plasma on wheat seed germination, seedling growth, osmotic-adjustment products, lipid peroxidation, reactive oxygen species (ROS), antioxidant enzyme activity, abscisic acid, and drought resistant related genes expression under drought stress were investigated. The changes of the wheat seed coat before and after the DBD plasma treatment were explored. Experimental results showed that the DBD plasma treatment could alleviate the adverse effects of drought stress on wheat seed germination and seedling growth; the germination potential and germination rate increased by 27...
November 30, 2017: Scientific Reports
S Bardiaux, D Martins, P Leclercq, M Pirson
INTRODUCTION: The aim of this study is (1) to describe the characteristics of the organ harvesting activity conducted in 2012 at the Erasme's Hospital, Brussels University Hospital, (2) to highlight the different combinations " type of donor/types of organ's retrieved " in relation to organ harvestings carried out within the hospital, and (3) to calculate the organ harvesting's cost of acts. METHODS: The study is conducted according to the hospital perspective. It assesses the consumption of medical and nursing staff resources, disposable material costs, medical device costs, drugs costs, sterile instruments and biomedical equipment costs, of the 34 organ harvesting procedures that has been conducted this year...
2017: Revue Médicale de Bruxelles
Simon Messer, Aravinda Page, Richard Axell, Marius Berman, Jules Hernández-Sánchez, Simon Colah, Barbora Parizkova, Kamen Valchanov, John Dunning, Evgeny Pavlushkov, Sendhil K Balasubramanian, Jayan Parameshwar, Yasir Abu Omar, Martin Goddard, Stephen Pettit, Clive Lewis, Anna Kydd, David Jenkins, Christopher J Watson, Catherine Sudarshan, Pedro Catarino, Marie Findlay, Ayyaz Ali, Steven Tsui, Stephen R Large
BACKGROUND: The requirement for heart transplantation is increasing, vastly outgrowing the supply of hearts available from donation after brain death (DBD) donors. Transplanting hearts after donation after circulatory-determined death (DCD) may be a viable additive alternative to DBD donors. This study compared outcomes from the largest single-center experience of DCD heart transplantation against matched DBD heart transplants. METHODS: DCD hearts were retrieved using normothermic regional perfusion (NRP) or direct procurement and perfusion (DPP)...
December 2017: Journal of Heart and Lung Transplantation
Dong Ju Son, Jie Zheng, Yu Yeon Jung, Chul Ju Hwang, Hee Pom Lee, Ju Rang Woo, Song Yi Baek, Young Wan Ham, Min Woong Kang, Minho Shong, Gi Ryang Kweon, Min Jong Song, Jae Kyung Jung, Sang-Bae Han, Bo Yeon Kim, Do Young Yoon, Bu Young Choi, Jin Tae Hong
Rationale: Signal transducer and activator of transcription-3 (STAT3) plays a pivotal role in cancer biology. Many small-molecule inhibitors that target STAT3 have been developed as potential anticancer drugs. While designing small-molecule inhibitors that target the SH2 domain of STAT3 remains the leading focus for drug discovery, there has been a growing interest in targeting the DNA-binding domain (DBD) of the protein. Methods: We demonstrated the potential antitumor activity of a novel, small-molecule (E)-2-methoxy-4-(3-(4-methoxyphenyl)prop-1-en-1-yl)phenol (MMPP) that directly binds to the DBD of STAT3, in patient-derived non-small cell lung cancer (NSCLC) xenograft model as well as in NCI-H460 cell xenograft model in nude mice...
2017: Theranostics
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