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https://www.readbyqxmd.com/read/28934494/derivatives-of-bst-like-gss-polymerase-with-improved-processivity-and-inhibitor-tolerance
#1
Igor P Oscorbin, Ekaterina A Belousova, Ulyana A Boyarskikh, Aleksandr I Zakabunin, Evgeny A Khrapov, Maksim L Filipenko
At the moment, one of the actual trends in medical diagnostics is a development of methods for practical applications such as point-of-care testing, POCT or research tools, for example, whole genome amplification, WGA. All the techniques are based on using of specific DNA polymerases having strand displacement activity, high synthetic processivity, fidelity and, most significantly, tolerance to contaminants, appearing from analysed biological samples or collected under purification procedures. Here, we have designed a set of fusion enzymes based on catalytic domain of DNA polymerase I from Geobacillus sp...
September 19, 2017: Nucleic Acids Research
https://www.readbyqxmd.com/read/28901448/exposure-of-keratinocytes-to-non%C3%A2-thermal-dielectric-barrier-discharge-plasma-increases-the-level-of-8%C3%A2-oxoguanine-via-inhibition-of-its-repair-enzyme
#2
Ki Cheon Kim, Madduma Hewage Susara Ruwan Kumara, Kyoung Ah Kang, Mei Jing Piao, Min Chang Oh, Yea Seong Ryu, Jin Oh Jo, Young Sun Mok, Jennifer H Shin, Yeunsoo Park, Seong Bong Kim, Suk Jae Yoo, Jin Won Hyun
Oxidative stress enhances cellular DNA oxidation and may cause mutations in DNA bases, including 8‑oxoguanine (8‑oxoG). Our recent study reported that exposure of cells to non‑thermal dielectric barrier discharge (DBD) plasma generates reactive oxygen species and damages DNA. The present study investigated the effect of non‑thermal DBD plasma exposure on the formation of 8‑oxoG in HaCaT human keratinocytes. Cells exposed to DBD plasma exhibited increased level of 8‑oxoG. In addition, mRNA and protein expression levels of 8‑oxoguanine glycosylase 1 (OGG1), an 8‑oxoG repair enzyme, were reduced in plasma‑exposed cells...
September 11, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28895214/highly-k-selective-fluorescent-probes-for-lifetime-sensing-of-k-in-living-cells
#3
Hans-Jürgen Holdt, Thomas Schwarze, Monique Mertens, Peter Müller, Janine Riemer, Pablo Wessig
The new K+ selective fluorescent probes 1 and 2 were obtained by CuAAC reactions of an alkyne substituted DBD ester fluorophore with azido functionalized N-phenylaza-18-crown-6 ether and N-(o-isopropoxy)-phenylaza-18-crown-6 ether, respectively. Probes 1 and 2 allow the detection of K+ in the presence of Na+ in water by fluorescence enhancement (2.2 for 1 at 2000 mM K+ and 2.5 for 2 at 160 mM K+). Fluorescence life time measurements in the absence and presence of K+ revealed bi-exponential decay kinetics with similar life times, however with different proportions changing the averaged fluorescence decay times (τf(av))...
September 12, 2017: Chemistry: a European Journal
https://www.readbyqxmd.com/read/28886219/comparison-of-outcomes-of-kidney-transplantation-from-donation-after-brain-death-donation-after-circulatory-death-and-donation-after-brain-death-followed-by-circulatory-death-donors
#4
Guodong Chen, Chang Wang, Dicken Shiu-Chung Ko, Jiang Qiu, Xiaopeng Yuan, Ming Han, Changxi Wang, Xiaoshun He, Lizhong Chen
There are three categories of deceased donors of kidney transplantation in China, donation after brain death(DBD), donation after circulatory death(DCD), donation after brain death followed by circulatory death(DBCD) donors. The aim of this study is to compare the outcomes of kidney transplantation from these three categories of deceased donors METHODS: We retrospectively reviewed 469 recipients who received deceased kidney transplantation in our hospital from February 2007 to June 2015. The recipients were divided into three groups according to the source of their donor kidneys: DBD, DCD or DBCD...
September 8, 2017: Clinical Transplantation
https://www.readbyqxmd.com/read/28885753/study-of-gas-phase-reactions-of-no2-with-aromatic-compounds-using-proton-transfer-reaction-time-of-flight-mass-spectrometry
#5
Jianquan Li, Xubing Du, Teng Guo, Zhen Peng, Li Xu, Junguo Dong, Ping Cheng, Zhen Zhou
The study of ion chemistry involving the NO2(+) is currently the focus of considerable fundamental interest and is relevant in diverse fields ranging from mechanistic organic chemistry to atmospheric chemistry. A very intense source of NO2(+) was generated by injecting the products from the dielectric barrier discharge (DBD) of a nitrogen and oxygen mixture upstream into the drift tube of a proton transfer reaction time-of-flight mass spectrometry (PTR-TOF-MS) apparatus with H3 O(+) as the reagent ion. The NO2(+) intensity is controllable and related to the DBD operation conditions and ratio of oxygen to nitrogen...
September 8, 2017: Journal of Mass Spectrometry: JMS
https://www.readbyqxmd.com/read/28885491/pancreas-transplantation-with-grafts-from-donors-deceased-after-circulatory-death-dcd-5-years-single-center-experience
#6
Wouter Kopp, Hwai-Ding Lam, A F M Schaapherder, Volkert Huurman, Paul van der Boog, Eelco de Koning, Johan W de Fijter, Andrzej Baranski, Andries Braat
INTRODUCTION: Donation after circulatory death (DCD) pancreas transplantation has been shown to be an additional way to deal with donor organ shortages. The results of 5-year DCD pancreas transplantation are presented. METHODS: A retrospective, single center analysis (2011 - 2015) was performed to compare the results of donation after brain death (DBD) to DCD pancreas transplantation. RESULTS: During the study period, 104 pancreas transplantations (83 from DBD and 21 from DCD) were performed...
September 6, 2017: Transplantation
https://www.readbyqxmd.com/read/28882731/danggui-buxue-decoction-promotes-angiogenesis-by-up-regulation-of-vegfr1-2-expressions-and-down-regulation-of-svegfr1-2-expression-in-myocardial-infarction-rat
#7
Guanying Hu, Peng Yang, Yu Zeng, Sanyin Zhang, Jiage Song
BACKGROUND: The traditional herbal compound Danggui Buxue decoction (DBD), has long been used for the prevention and treatment of cardiovascular diseases, however, the underlying molecular mechanism for its effect remains still unknown. So this study would to investigate the effect of DBD on cardiac damage induced by myocardial infarction (MI) challenge. METHODS: SD Rats with ligation of left anterior descending (LAD) coronary artery were randomly divided into MI, MI plus Betaloc Zok, MI plus DBD high dose, and MI plus DBD low dose group, together with sham-operated group...
September 4, 2017: Journal of the Chinese Medical Association: JCMA
https://www.readbyqxmd.com/read/28875352/specificity-of-reward-sensitivity-and-parasympathetic-based-regulation-among-children-with-attention-deficit-hyperactivity-and-disruptive-behavior-disorders
#8
Rachel B Tenenbaum, Erica D Musser, Joseph S Raiker, Erika K Coles, Elizabeth M Gnagy, William E Pelham
Attention-deficit/hyperactivity disorder (ADHD) is associated with disruptionsin reward sensitivity and regulatory processes. However, it is unclear whether thesedisruptions are better explained by comorbid disruptive behavior disorder (DBD)symptomology. This study sought to examine this question using multiple levels ofanalysis (i.e., behavior, autonomic reactivity). One hundred seventeen children (aged 6 to 12 years; 72.6% male; 69 with ADHD) completed theBalloon-Analogue Risk Task (BART) to assess external reward sensitivity behaviorally...
September 6, 2017: Journal of Abnormal Child Psychology
https://www.readbyqxmd.com/read/28871438/the-structure-splicing-synteny-and-expression-of-lamprey-coe-genes-and-the-evolution-of-the-coe-gene-family-in-chordates
#9
Ricardo Lara-Ramírez, Guillaume Poncelet, Cédric Patthey, Sebastian M Shimeld
COE genes encode transcription factors that have been found in all metazoans examined to date. They possess a distinctive domain structure that includes a DNA-binding domain (DBD), an IPT/TIG domain and a helix-loop-helix (HLH) domain. An intriguing feature of the COE HLH domain is that in jawed vertebrates it is composed of three helices, compared to two in invertebrates. We report the isolation and expression of two COE genes from the brook lamprey Lampetra planeri and compare these to COE genes from the lampreys Lethenteron japonicum and Petromyzon marinus...
September 5, 2017: Development Genes and Evolution
https://www.readbyqxmd.com/read/28869600/foxo3-mediated-chemo-protection-in-high-stage-neuroblastoma-depends-on-wild-type-tp53-and-sesn3
#10
M Rupp, J Hagenbuchner, B Rass, H Fiegl, U Kiechl-Kohlendorfer, P Obexer, M J Ausserlechner
Forkhead box O class transcription factors are homeostasis regulators that control cell death, longevity and therapy-resistance. In neuroblastoma (NB), nuclear FOXO3 correlates with stage M disease and poor prognosis. To analyze whether FOXO3 contributes to drug-resistance in this childhood cancer, we investigated how different high-stage-derived NB cells respond to the activation of an ectopic FOXO3 allele. We found endogenous FOXO3 mostly localized to the nucleus-upon activation of an ectopic, 4OHT-activated FOXO3(A3)ER fusion protein two of the cell lines underwent apoptosis, whereas in the others FOXO3-activation even increased survival during drug-treatment...
September 4, 2017: Oncogene
https://www.readbyqxmd.com/read/28866716/blood-serum-concentrations-of-kynurenic-acid-in-patients-diagnosed-with-recurrent-depressive-disorder-depression-in-bipolar-disorder-and-schizoaffective-disorder-treated-with-electroconvulsive-therapy
#11
Marcin Olajossy, Bartosz Olajossy, Sebastian Wnuk, Emilia Potembska, Ewa Urbańska
OBJECTIVES: The aim of the present study was to compare blood serum kynurenic acid (KYNA) concentrations measured before ECT and after 1, 6 and 12 electroconvulsive treatment (ECT) sessions in patients with diagnoses of recurrent depressive disorder (RDD), depression in bipolar disorder (DBD) and schizoaffective disorder (SAD). METHODS: The study group comprised of 50 patients with ICD-10 diagnoses of RDD, DBD and SAD. Blood serum KYNA concentrations were determined and clinical assessment was performed using the MADRS and the GAF scale...
June 18, 2017: Psychiatria Polska
https://www.readbyqxmd.com/read/28860187/analysis-of-dna-binding-by-human-factor-xeroderma-pigmentosum-complementation-group-a-xpa-provides-insight-into-its-interactions-with-nucleotide-excision-repair-substrates
#12
Norie Sugitani, Markus W Voehler, Michelle S Roh, Agnieszka M Topolska-Woś, Walter J Chazin
Xeroderma pigmentosum (XP) complementation group A (XPA) is an essential scaffolding protein in the multi-protein nucleotide excision repair (NER) machinery. The interaction of XPA with DNA is a core function of this protein; a number of mutations in the DNA binding domain (DBD) are associated with XP disease. Although structures of the central globular domain of human XPA and data on binding of DNA substrates have been reported, the structural basis for XPAs DNA binding activity remains unknown. X-ray crystal structures of the central globular domain of yeast XPA (Rad14) with lesion-containing DNA duplexes have provided valuable insights, but the DNA substrates used for this study do not correspond to the substrates of XPA as it functions within the NER machinery...
August 31, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/28842371/molecular-cloning-and-functional-characterization-of-interferon-regulatory-factor-7-of-the-barbel-chub-squaliobarbus-curriculus
#13
Xin Zhao, Ronghua Wang, Yaoguo Li, Tiaoyi Xiao
The interferon regulatory factor 7 (IRF7) is a critical regulator of type-I interferon-dependent immune reaction that defense against virus. To investigate the antiviral function of IRF7 of barbel chub Squaliobarbus curriculus (ScIRF7), the cDNA of ScIRF7 was cloned and characterized. The full length cDNA of ScIRF7 was 1870 bp, consisted of 41 bp 5'-UTR, 560 bp 3'-UTR and a 1269 bp open reading frame (ORF). The ORF encoded 423 amino acids with a molecular weight of 49.426 KDa and a theoretical isoelectric point of 5...
August 24, 2017: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/28840968/naphthalene-biodegradation-under-oxygen-limiting-conditions-community-dynamics-and-the-relevance-of-biofilm-forming-capacity
#14
Sophie-Marie Martirani-Von Abercron, Patricia Marín, Marta Solsona-Ferraz, Mayra-Alejandra Castañeda-Cataña, Silvia Marqués
Toxic polycyclic aromatic hydrocarbons (PAHs) are frequently released into the environment from anthropogenic sources. PAH remediation strategies focus on biological processes mediated by bacteria. The availability of oxygen in polluted environments is often limited or absent, and only bacteria able to thrive in these conditions can be considered for bioremediation strategies. To identify bacterial strains able to degrade PAHs under oxygen-limiting conditions, we set up enrichment cultures from samples of an oil-polluted aquifer, using either anoxic or microaerophilic condition and with PAHs as the sole carbon source...
August 25, 2017: Microbial Biotechnology
https://www.readbyqxmd.com/read/28838607/structural-and-biochemical-characterization-of-ligand-recognition-by-cysb-the-master-regulator-of-sulfate-metabolism
#15
Monica Mittal, Appu Kumar Singh, S Kumaran
CysB, a member of LysR-type transcriptional regulators, up-regulates the expression of genes associated with sulfate metabolism and cysteine biosynthesis. CysB is activated under sulfur limiting conditions by O-acetylserine (OAS) and N-acetylserine (NAS), but the activation mechanism of CysB remain unknown. Here, we report four crystal structures of ligand binding domains of CysB (CysB-LBD) in apo form and in complex with sulfate, OAS, and NAS. Our results show that CysB has two distinct allosteric ligand binding sites; a sulfate and NAS specific site-1 and a second, NAS and OAS specific site-2...
August 21, 2017: Biochimie
https://www.readbyqxmd.com/read/28836426/controlling-the-growth-of-molecular-crystal-aggregates-with-distinct-linear-and-nonlinear-optical-properties
#16
Yusen Luo, Chunqing Yuan, Jialiang Xu, Yongjun Li, Huibiao Liu, Sergey Semin, Theo Rasing, Wensheng Yang, Yuliang Li
Two novel donor-acceptor molecules, 2,7-diphenylbenzo[1,2-b:4,3-b']difuran-4,5-dicarbonitrile and 2,7-bis(4-methoxyphenyl)benzo[1,2-b:4,3-b']difuran-4,5-dicarbonitrile containing cyano group as the electron acceptor, were synthesized. Their single-crystal structures, molecular packing, and self-assembly behaviors were also investigated. By simple solvent evaporation techniques, these compounds self-assemble into various low-dimensional microstructures that demonstrate distinctive nonlinear optical properties depending on the orientations of their transition dipoles...
September 13, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28834180/attitudes-and-barriers-to-the-use-of-dcd-livers-comparison-of-a-u-s-transplant-center-survey-to-the-united-network-for-organ-sharing-data
#17
Linda Sher, Cristiano Quintini, Sameh Adel Fayek, Peter Abt, Mary Lo, Pui Yan, Lingyun Ji, Susan Groshen, Jamie Case, Christopher Lee Marsh
Transplantation of liver grafts from donation after circulatory death (DCD) is limited. To identify barriers of DCD livers utilization, all active United States (U.S.) liver transplant centers (n=138) were surveyed and responses compared to the United Network for Organ Sharing (UNOS) data. Seventy-four centers (54%) responded, and diversity in attitudes was observed, with many not using organ and/or recipient prognostic variables defined in prior studies and UNOS data analysis. Most centers (74%) believed lack of a system allowing a timely re-transplant is a barrier to utilization...
August 17, 2017: Liver Transplantation
https://www.readbyqxmd.com/read/28834150/atmospheric-pressure-non-thermal-plasma-activation-of-co2-in-a-packed-bed-dielectric-barrier-discharge-reactor
#18
Danhua Mei, Xin Tu
Direct conversion of CO2 into CO and O2 was carried out in a packed-bed dielectric barrier discharge (DBD) non-thermal plasma reactor at low temperatures and atmospheric pressure. The maximum CO2 conversion of 22.6% was achieved when BaTiO3 pellets were fully packed into the discharge gap. The introduction of γ-Al2O3 or 10 wt.% Ni/γ-Al2O3 into the BaTiO3 packed DBD reactor increased both CO2 conversion and energy efficiency of the plasma process. Packing γ-Al2O3 or 10 wt.% Ni/γ-Al2O3 in the upstream of the BaTiO3 bed showed higher CO2 conversion and energy efficiency compared to that using middle or downstream packing modes as the reverse reaction of CO2 conversion - the recombination of CO and O to form CO2 is more likely to happen in the middle and downstream modes...
August 17, 2017: Chemphyschem: a European Journal of Chemical Physics and Physical Chemistry
https://www.readbyqxmd.com/read/28827762/formation-of-side-discharges-in-dielectric-barrier-discharge
#19
Weili Fan, Zhengming Sheng, Lifang Dong, Fucheng Liu, Xiaoxia Zhong, Yiqian Cui, Fang Hao, Tian Du
Pattern formation and self-organization are fascinating phenomena found widely in nature and in laboratory environment such as dielectric barrier discharge (DBD). Significant efforts have been made to explain the dynamic pattern formation. In DBD, the formation of side discharges is generally supposed to be a key factor responsible for diversity and spatial-temporal symmetry breaking of pattern formation. However, it is still not clear how such discharges are induced. Here, we present the observations of side discharges in a filamentary dielectric barrier discharge from both numerical simulations and experiments...
August 21, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28821765/the-protective-action-of-osmolytes-on-the-deleterious-effects-of-gamma-rays-and-atmospheric-pressure-plasma-on-protein-conformational-changes
#20
Pankaj Attri, Minsup Kim, Thapanut Sarinont, Eun Ha Choi, Hyunwoong Seo, Art E Cho, Kazunori Koga, Masaharu Shiratani
Both gamma rays and atmospheric pressure plasma are known to have anticancer properties. While their mechanism actions are still not clear, in some contexts they work in similar manner, while in other contexts they work differently. So to understand these relationships, we have studied Myoglobin protein after the treatment of gamma rays and dielectric barrier discharge (DBD) plasma, and analyzed the changes in thermodynamic properties and changes in the secondary structure of protein after both treatments. The thermodynamic properties were analyzed using chemical and thermal denaturation after both treatments...
August 18, 2017: Scientific Reports
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