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https://www.readbyqxmd.com/read/28225083/antitumorigenic-effect-of-atmospheric-pressure-dielectric-barrier-discharge-on-human-colorectal-cancer-cells-via-regulation-of-sp1-transcription-factor
#1
Duksun Han, Jin Hyoung Cho, Ra Ham Lee, Woong Bang, Kyungho Park, Minseok S Kim, Jung-Hyun Shim, Jung-Il Chae, Se Youn Moon
Human colorectal cancer cell lines (HT29 and HCT116) were exposed to dielectric barrier discharge (DBD) plasma at atmospheric pressure to investigate the anticancer capacity of the plasma. The dose- and time-dependent effects of DBDP on cell viability, regulation of transcription factor Sp1, cell-cycle analysis, and colony formation were investigated by means of MTS assay, DAPI staining, propidium iodide staining, annexin V-FITC staining, Western blot analysis, RT-PCR analysis, fluorescence microscopy, and anchorage-independent cell transformation assay...
February 22, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28216490/-expression-and-purification-of-foxo1-dna-binding-domain-and-its-dna-properties
#2
Yinuer Ha, Jun Li, Yongheng Chen, Lin Chen, Zhuchu Chen
To express and purify FOXO1 DNA binding domain (DBD) and to evaluate its DNA binding properties.
 Methods: Gene sequence of FOXO1-DBD was optimized and the recombinant strains were induced at low temperature to obtain soluble protein of FOXO1-DBD. Then, FOXO1-DBD protein was purified through Ni affinity chromatography and cation exchange. Finally, the DNA binding properties of FOXO1-DBD was evaluated by electrophoretic mobility shift assay (EMSA).
 Results: Most of soluble proteins were obtained by optimizing its genes sequence and induction at 21 ℃...
January 28, 2017: Zhong Nan da Xue Xue Bao. Yi Xue Ban, Journal of Central South University. Medical Sciences
https://www.readbyqxmd.com/read/28207629/short-and-long-term-outcome-after-lung-transplantation-from-circulatory-dead-donors-a-single-center-experience
#3
David Ruttens, An Martens, Sofie Ordies, Stijn E Verleden, Arne P Neyrinck, Robin Vos, Marc Boada Collado, Bart M Vanaudenaerde, Geert M Verleden, Dirk Van Raemdonck
BACKGROUND: Donation after cardiac death (DCD) to overcome the donor organ shortage is well accepted in the clinical setting, although long-term outcome after DCD lung transplantation (LTx) remains largely unknown. METHODS: In this retrospective study, DCD LTx recipients (n=59) were compared with a cohort of donation after brain-death (DBD) LTx recipients (n=331) transplanted between February 2007 and September 2013; follow-up was until January 1st 2016. Short-term (duration of mechanical ventilation, ICU stay, hospital stay and highest PGD score within 72 hours) and long-term (CLAD-free and overall survival) follow-up were compared over a median follow-up of 50...
February 15, 2017: Transplantation
https://www.readbyqxmd.com/read/28192915/portable-dielectric-barrier-discharge-atomic-emission-spectrometer
#4
Na Li, Zhongchen Wu, Yingying Wang, Jing Zhang, Xiangnan Zhang, Hengnan Zhang, Wenhai Wu, Jing Gao, Jie Jiang
This paper describes the first demonstration of a portable dielectric barrier discharge-atomic emission spectrometer (DBD-AES). The instrument primarily consists of a miniature electro-thermal vaporizer (ETV), DBD, and optical signal acquisition units. It weighs only 4.5 kg and is powered by a 24 V DC battery with a maximum power consumption of 37 W. The accompanying software can be operated on a laptop computer. A specially designed quartz tube integrates the ETV unit with the DBD chamber. The effects of experimental parameters were investigated...
February 7, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28181368/diamond-burr-debridement-vs-grid-keratotomy-in-canine-scced-with-scanning-electron-microscopy-diamond-burr-tip-analysis
#5
Chloe B Spertus, Josef M Brown, Elizabeth A Giuliano
OBJECTIVES: (i) Compare the outcome of canine SCCED treated with grid keratotomy (GK) or diamond burr debridement (DBD); (ii) Serially evaluate morphologic and elemental composition changes to diamond burr tips. ANIMALS STUDIED: A total of 91 eyes of 88 canine SCCED patients treated at the University of Missouri (2005-2015); 75 fresh cadaver porcine globes. PROCEDURES: (i) Medical records were reviewed retrospectively. Data were analyzed for age, sex, breed, procedure performed, eye(s) on which the procedure was performed, time to healing after a single surgical procedure, performance of a second surgical procedure, contact lens placement, and postprocedural complications...
February 9, 2017: Veterinary Ophthalmology
https://www.readbyqxmd.com/read/28168148/protein-functionalized-dna-nanostructures-as-tools-to-control-transcription-in-zebrafish-embryos
#6
Alessandro Angelin, Olivier Kassel, Sepand Rastegar, Uwe Strähle, Christof M Niemeyer
The unique structure-directing properties of DNA origami nanostructures (DONs) show great potential to specifically manipulate intracellular processes. We report an innovative concept to selectively activate the transcription of a single gene in the developing zebrafish embryo. We reason that engineering a designer transcription factor in which a rigid DON imposes a fixed distance between the DNA-binding domain (DBD) and the transactivation domain (TAD) would allow the selective activation of a gene harboring the same distance between the corresponding transcription factor binding site and the core promoter...
February 2017: ChemistryOpen
https://www.readbyqxmd.com/read/28166240/involvement-of-multiple-stressors-induced-by-non-thermal-plasma-charged-aerosols-during-inactivation-of-airborne-bacteria
#7
Nachiket D Vaze, Sin Park, Ari D Brooks, Alexander Fridman, Suresh G Joshi
A lab-scale, tunable, single-filament, point-to-point nonthermal dieletric-barrier discharge (DBD) plasma device was built to study the mechanisms of inactivation of aerosolized bacterial pathogens. The system inactivates airborne antibiotic-resistant pathogens efficiently. Nebulization mediated pre-optimized (4 log and 7 log) bacterial loads were challenged to plasma-charged aerosols, and lethal and sublethal doses determined using colony assay, and cell viability assay; and the loss of membrane potential and cellular respiration were determined using cell membrane potential assay and XTT assay...
2017: PloS One
https://www.readbyqxmd.com/read/28163377/destruction-of-biological-particles-using-non-thermal-plasma
#8
REVIEW
Akira Mizuno
Mechanism of inactivation of bio-particles exposed to non-thermal plasma (NTP), namely, dielectric barrier discharge (DBD), and plasma jet (PJ), has been studied using E. coli, B. subtilis spore, S. cerevisiae and bacteriophages. States of different biological components were monitored during the course of inactivation. Analysis of green fluorescent protein, GFP, introduced into E. coli. or B. subtiles spore cells proved that radicals generated by NTP penetrate into microbes, destroying the cell membrane and finally damage the genes...
January 2017: Journal of Clinical Biochemistry and Nutrition
https://www.readbyqxmd.com/read/28139537/are-childhood-externalizing-disorders-the-harbinger-of-early-onset-alcohol-dependence
#9
Abhishek Ghosh, Savita Malhotra, Debasish Basu
BACKGROUND & OBJECTIVES: The subtyping of alcohol dependence (AD) into early-onset (EO) and late-onset (LO) subgroups has been shown to have clinical and biological validity. As externalizing disorders (EDs) predate AD, the link of ED with age of onset of alcohol dependence needs to be investigated. The aim of this study was to examine the relationship of EDs such as disruptive behaviour disorder (DBD) and attention deficit hyperactivity disorder (ADHD) with age at onset of AD in a sample of male subjects...
September 2016: Indian Journal of Medical Research
https://www.readbyqxmd.com/read/28126403/binding-of-amphipathic-cell-penetrating-peptide-p28-to-wild-type-and-mutated-p53-as-studied-by-raman-atomic-force-and-surface-plasmon-resonance-spectroscopies
#10
Sara Signorelli, Simona Santini, Tohru Yamada, Anna Rita Bizzarri, Craig W Beattie, Salvatore Cannistraro
BACKGROUND: Mutations within the DNA binding domain (DBD) of the tumor suppressor p53 are found in >50% of human cancers and may significantly modify p53 secondary structure impairing its function. p28, an amphipathic cell-penetrating peptide, binds to the DBD through hydrophobic interaction and induces a posttranslational increase in wildtype and mutant p53 restoring functionality. We use mutation analyses to explore which elements of secondary structure may be critical to p28 binding...
January 24, 2017: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/28111805/ureteric-complications-in-recipients-of-kidneys-from-donation-after-circulatory-death-donors
#11
Trina-Jo Mah, Dermot H Mallon, Oliver Brewster, Kourosh Saeb-Parsy, Andrew J Butler, J Andrew Bradley, Vasilis Kosmoliaptsis
A large increase in the use of kidneys from donation after circulatory death (DCD) donors prompted us to examine the impact of donor type on the incidence of ureteric complications (UCs; ureteric stenosis, urinary leak) after kidney transplantation. We studied 1072 consecutive kidney transplants [DCD n=494, live-donor (LD) n=273, donation after brain death (DBD) n=305] performed during 2008 to 2014. Overall, there was a low incidence of UCs after kidney transplantation (3.5%). Despite a trend towards higher incidence of UCs in DCD (n=22, 4...
January 23, 2017: Clinical Transplantation
https://www.readbyqxmd.com/read/28087460/identification-of-the-doublesex-protein-binding-sites-that-activate-expression-of-lozenge-in-the-female-genital-disc-in-drosophila-melanogaster
#12
Shunsuke Wagamitsu, Dan Takase, Fugaku Aoki, Masataka G Suzuki
Normal sexual differentiation in the genital organs is essential for the animal species that use sexual reproduction. Although it is known that doublesex (dsx) is required for the sexual development of the genitalia in various insect species, the direct target genes responsible for the sexual differentiation of the genitalia have not been identified. The lozenge (lz) gene is expressed in the female genital disc and is essential for developments of spermathecae and accessory glands in Drosophila melanogaster...
January 10, 2017: Mechanisms of Development
https://www.readbyqxmd.com/read/28077791/carcinoma-risk-variant-of-ebna1-deregulates-epstein-barr-virus-episomal-latency
#13
Jayaraju Dheekollu, Kimberly Malecka, Andreas Wiedmer, Henri-Jacques Delecluse, Alan K S Chiang, Dario C Altieri, Troy E Messick, Paul M Lieberman
Epstein-Barr Virus (EBV) latent infection is a causative co-factor for endemic Nasopharyngeal Carcinoma (NPC). NPC-associated variants have been identified in EBV-encoded nuclear antigen EBNA1. Here, we solve the X-ray crystal structure of an NPC-derived EBNA1 DNA binding domain (DBD) and show that variant amino acids are found on the surface away from the DNA binding interface. We show that NPC-derived EBNA1 is compromised for DNA replication and episome maintenance functions. Recombinant virus containing the NPC EBNA1 DBD are impaired in their ability to immortalize primary B-lymphocytes and suppress lytic transcription during early stages of B-cell infection...
January 31, 2017: Oncotarget
https://www.readbyqxmd.com/read/28045389/crystal-structure-of-an-iclr-homologue-from-microbacterium-sp-strain-hm58-2
#14
Tomonori Akiyama, Yusuke Yamada, Naoki Takaya, Shinsaku Ito, Yasuyuki Sasaki, Shunsuke Yajima
The bacterial transcription factor IclR (isocitrate lyase regulator) is a member of a one-component signal transduction system, which shares the common motif of a helix-turn-helix (HTH)-type DNA-binding domain (DBD) connected to a substrate-binding domain (SBD). Here, the crystal structure of an IclR homologue (Mi-IclR) from Microbacterium sp. strain HM58-2, which catabolizes acylhydrazide as the sole carbon source, is reported. Mi-IclR is expected to regulate an operon responsible for acylhydrazide degradation as an initial step...
January 1, 2017: Acta Crystallographica. Section F, Structural Biology Communications
https://www.readbyqxmd.com/read/28044425/immediate-effects-of-diamond-burr-debridement-in-patients-with-spontaneous-chronic-corneal-epithelial-defects-light-and-electron-microscopic-evaluation
#15
Charlotte Dawson, Carolina Naranjo, Belen Sanchez-Maldonado, Georgina V Fricker, Rose N Linn-Pearl, Natalia Escanilla, Christiane Kafarnik, David J Gould, Rick F Sanchez, Marian Matas-Riera
PURPOSE: To evaluate immediate effects of diamond burr debridement (DBD) on the cornea of canine patients diagnosed with spontaneous chronic corneal epithelial defects (SCCEDs). ANIMALS STUDIED: Eight client owned dogs with SCCEDs. METHODS: Nine eyes from eight dogs with SCCEDs underwent superficial keratectomy (SK). The ulcerated area was divided into quadrants with a 300-micron restricted depth knife. Two of four quadrants underwent DBD for 40-60 s...
January 2017: Veterinary Ophthalmology
https://www.readbyqxmd.com/read/28027614/decision-modelling-in-donation-after-circulatory-death-dcd-liver-transplantation
#16
K A McLean, J Camilleri-Brennan, S R Knight, T Drake, R Ots, C A Shaw, S J Wigmore, E M Harrison
INTRODUCTION: Donation after circulatory death (DCD) liver allografts are increasingly used for transplantation. However, the post-transplantation clinical and quality of life outcomes of DCD recipients are traditionally considered to be inferior compared with donation after brain death (DBD) allograft recipients. Decision making for such marginal organs can be difficult. This study investigated the optimal decision to accept or decline a DCD liver allograft for a patient based on their current health...
December 27, 2016: Liver Transplantation
https://www.readbyqxmd.com/read/28027600/comparison-of-long-term-outcomes-and-quality-of-life-in-recipients-of-donation-after-cardiac-death-liver-grafts-with-a-propensity-matched-cohort
#17
K P Croome, D D Lee, D K Perry, J M Burns, J H Nguyen, A P Keaveny, C B Taner
BACKGROUND: The use of liver grafts from donation after cardiac death donors (DCD LT) has been limited due to the increased rate of graft failure, mostly related to ischemic cholangiopathy(IC). It is our hypothesis that long term outcomes and quality of life similar to patients undergoing liver transplantation with donation after brain death donors (DBD LT) can be achieved. METHODS: Clinical outcomes of all patients undergoing DCD LT (n = 300) between 1998-2015 were compared to a propensity score matched cohort of patients undergoing DBD LT (n = 300)...
December 27, 2016: Liver Transplantation
https://www.readbyqxmd.com/read/28012972/a-novel-peptide-9r-p201-strongly-inhibits-the-viability-proliferation-and-migration-of-liver-cancer-hepg2-cells-and-induces-apoptosis-by-down-regulation-of-foxm1-expression
#18
Zhenfei Bi, Wenrong Liu, Ruofang Ding, Yiran Wu, Rongkun Dou, Wenwen Zhang, Xue Yuan, Xinrong Liu, Lili Xiong, Zhiyun Guo, Canquan Mao
Overexpression of FoxM1 was closely related to the proliferation, metastasis, chemo-resistance and poor prognosis of various cancers. FoxM1 was regarded as the Achilles' heel of cancer and a potential target for anti-cancer drug discovery. We previously obtained several high affinity peptides from the phage random library against the DNA binding domain of FoxM1c (FoxM1c-DBD) protein. Here in this paper, we found that 9R-P201, one of the novel peptides, showed stronger inhibition to HepG2 cancer cells than those of DU145, HUVEC and L-02 cells with an IC50 of 43...
December 21, 2016: European Journal of Pharmacology
https://www.readbyqxmd.com/read/28011069/alanine-scanning-mutagenesis-of-human-signal-transducer-and-activator-of-transcription-1-to-estimate-loss-or-gain-of-function-variants
#19
Reiko Kagawa, Ryoji Fujiki, Miyuki Tsumura, Sonoko Sakata, Shiho Nishimura, Yuval Itan, Xiao-Fei Kong, Zenichiro Kato, Hidenori Ohnishi, Osamu Hirata, Satoshi Saito, Maiko Ikeda, Jamila El Baghdadi, Aziz Bousfiha, Kaori Fujiwara, Matias Oleastro, Judith Yancoski, Laura Perez, Silvia Danielian, Fatima Ailal, Hidetoshi Takada, Toshiro Hara, Anne Puel, Stéphanie Boisson-Dupuis, Jacinta Bustamante, Jean-Laurent Casanova, Osamu Ohara, Satoshi Okada, Masao Kobayashi
BACKGROUND: Germline heterozygous mutations in human signal transducer and activator of transcription 1 (STAT1) can cause loss of function (LOF), as in patients with Mendelian susceptibility to mycobacterial diseases, or gain of function (GOF), as in patients with chronic mucocutaneous candidiasis. LOF and GOF mutations are equally rare and can affect the same domains of STAT1, especially the coiled-coil domain (CCD) and DNA-binding domain (DBD). Moreover, 6% of patients with chronic mucocutaneous candidiasis with a GOF STAT1 mutation have mycobacterial disease, obscuring the functional significance of the identified STAT1 mutations...
December 14, 2016: Journal of Allergy and Clinical Immunology
https://www.readbyqxmd.com/read/27991548/non-thermal-atmospheric-pressure-plasma-efficiently-promotes-the-proliferation-of-adipose-tissue-derived-stem-cells-by-activating-no-response-pathways
#20
Jeongyeon Park, Hyunyoung Lee, Hae June Lee, Gyoo Cheon Kim, Do Young Kim, Sungbum Han, Kiwon Song
Non-thermal atmospheric pressure plasma (NTAPP) is defined as a partially ionized gas with electrically charged particles at atmospheric pressure. Our study showed that exposure to NTAPP generated in a helium-based dielectric barrier discharge (DBD) device increased the proliferation of adipose tissue-derived stem cells (ASCs) by 1.57-fold on an average, compared with untreated cells at 72 h after initial NTAPP exposure. NTAPP-exposed ASCs maintained their stemness, capability to differentiate into adipocytes but did not show cellular senescence...
December 19, 2016: Scientific Reports
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