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https://www.readbyqxmd.com/read/27921202/endogenous-stem-cells-were-recruited-by-defocused-low-energy-shock-wave-in-treating-diabetic-bladder-dysfunction
#1
Yang Jin, Lina Xu, Yong Zhao, Muwen Wang, Xunbo Jin, Haiyang Zhang
Defocused low-energy shock wave (DLSW) has been shown effects on activating mesenchymal stromal cells (MSCs) in vitro. In this study, recruitment of endogenous stem cells was firstly examined as an important pathway during the healing process of diabetic bladder dysfunction (DBD) treated by DLSW in vivo. Neonatal rats received intraperitoneal injection of 5-ethynyl-2-deoxyuridine (EdU) and then DBD rat model was created by injecting streptozotocin. Four weeks later, DLSW treatment was performed. Afterward, their tissues were examined by histology...
December 5, 2016: Stem Cell Reviews
https://www.readbyqxmd.com/read/27918103/identifying-opportunities-to-increase-organ-donation-after-brain-death-an-observational-study-in-sweden-2009-2014
#2
T Nolin, C Mårdh, G Karlström, S M Walther
BACKGROUND: There is a wide gap between the number of organ donors and patients on waiting lists for transplantation. The purpose of this Swedish nationwide study of the critical pathway for organ donation after brain death (DBD) was to identify missed opportunities for organ donation. METHODS: We performed a prospective, observational study of all ICU deaths in Sweden from Jan 1, 2009 to Dec 31, 2014. The protocol structure followed the critical pathway for organ donation, which was developed and tested during 2008...
January 2017: Acta Anaesthesiologica Scandinavica
https://www.readbyqxmd.com/read/27916030/-short-term-outcomes-of-lung-transplant-recipients-using-organs-from-brain-death-donors
#3
W X He, C Jiang, X G Liu, W Huang, C Chen, L Jiang, B Yang, K Wu, Q K Chen, Y Yang, Y M Yu, G N Jiang
Objective: To assess short-term outcomes after lung transplantation with organs procured following brain death. Methods: Between April 2015 and July 2016, all 17 recipients after lung transplantation using organs from brain death donors (DBD) at Department of Thoracic Surgery, Shanghai Pulmonary Hospital, Tongji University School of Medicine were enrolled in this study. All patients were male, aging (60±7) years, including 11 chronic obstructive pulmonary disease, 5 idiopathic pulmonary fibrosis, 1 silicosis...
December 1, 2016: Zhonghua Wai Ke za Zhi [Chinese Journal of Surgery]
https://www.readbyqxmd.com/read/27900274/stochastic-resonance-whole-body-vibration-increases-perceived-muscle-relaxation-but-not-cardiovascular-activation-a-randomized-controlled-trial
#4
Achim Elfering, Christian Burger, Volker Schade, Lorenz Radlinger
AIM: To investigate the acute effects of stochastic resonance whole body vibration (SR-WBV), including muscle relaxation and cardiovascular activation. METHODS: Sixty-four healthy students participated. The participants were randomly assigned to sham SR-WBV training at a low intensity (1.5 Hz) or a verum SR-WBV training at a higher intensity (5 Hz). Systolic blood pressure (SBP), diastolic blood pressure (DBP), heart rate (HR) and self-reported muscle relaxation were assessed before and immediately after SR-WBV...
November 18, 2016: World Journal of Orthopedics
https://www.readbyqxmd.com/read/27890717/ecdysone-receptor-ecr-and-ultraspiracle-usp-genes-from-the-cyclopoid-copepod-paracyclopina-nana-identification-and-expression-in-response-to-water-accommodated-fractions-wafs
#5
Jayesh Puthumana, Min-Chul Lee, Jeonghoon Han, Hui-Su Kim, Dae-Sik Hwang, Jae-Seong Lee
Ecdysteroid hormones are pivotal in the development, growth, and molting of arthropods, and the hormone pathway is triggered by binding ecdysteroid to a heterodimer of the two nuclear receptors; ecdysone receptors (EcR) and ultraspiracle (USP). We have characterized EcR and USP genes, and their 5'-untranslated region (5'-UTR) from the copepod Paracyclopina nana, and studied mRNA transcription levels in post-embryonic stages and in response to water accommodated fractions (WAFs) of crude oil. The open reading frames (ORF) of EcR and USP were 1470 and 1287bp that encoded 490 and 429 amino acids with molecular weight of 121...
November 24, 2016: Comparative Biochemistry and Physiology. Toxicology & Pharmacology: CBP
https://www.readbyqxmd.com/read/27885385/in-package-inactivation-of-pathogenic-and-spoilage-bacteria-associated-with-poultry-using-dielectric-barrier-discharge-cold-plasma-treatments
#6
Michael J Rothrock, Hong Zhuang, Kurt C Lawrence, Brian C Bowker, Gary R Gamble, Kelli L Hiett
The goal of this study was to test the efficacy of in-package dielectric barrier discharge-cold plasma (DBD-CP) treatment to inactivate poultry-associated spoilage (Pseudomonas fluorescens) and pathogenic (Salmonella enterica Typhimurium, Campylobacter jejuni) bacteria. Liquid cultures of the bacterial isolates were sealed within packages containing ambient air (Trial 1) or modified air (65% O2:30% CO2:5% N2; Trial 2). The packages were subjected to treatment times ranging from 30 to 180 s, and after 24 h incubation at 4 °C, bacterial titers were determined...
November 24, 2016: Current Microbiology
https://www.readbyqxmd.com/read/27874968/professionalization-of-surgical-abdominal-organ-recovery-leading-to-an-increase-in-pancreatic-allografts-accepted-for-transplantation-in-the-netherlands-a-serial-analysis
#7
H D Lam, A F Schaapherder, W H Kopp, H Putter, A E Braat, A Baranski
Professional abdominal organ recovery with certification has been mandatory in the Netherlands since 2010. This study analyses the effects of certification (January 2010 - September 2015) on pancreas transplantation and compares it to an era before certification (February 2002 - May 2008) for surgical injuries and the number of pancreases transplanted. A total of 264 cases were analysed. Eighty-four recovered pancreases (31.8%) with surgically injuries were encountered. Forty six of those were surgically salvaged for transplantation, resulting in a total of 226 (85...
November 22, 2016: Transplant International: Official Journal of the European Society for Organ Transplantation
https://www.readbyqxmd.com/read/27864895/the-postreperfusion-syndrome-is-associated-with-acute-kidney-injury-following-donation-after-brain-death-liver-transplantation
#8
Marit Kalisvaart, Jubi E de Haan, Dennis A Hesselink, Wojciech G Polak, Bettina E Hansen, Jan N M IJzermans, Diederik Gommers, Herold J Metselaar, Jeroen de Jonge
Acute kidney injury (AKI) is frequently observed after DBD liver transplantation (LT) and associated with impaired recipient survival and chronic kidney disease. Hepatic ischemia/reperfusion injury (IRI) is suggested to be an important factor in this process. The postreperfusion syndrome (PRS) is the first manifestation of severe hepatic IRI directly after reperfusion. We performed a retrospective study on the relation between hepatic IRI and PRS and their impact on AKI in 155 DBD LT recipients. Severity of hepatic IRI was measured by peak postoperative AST levels and PRS was defined as >30% decrease in MAP ≥1 minute <5 minutes after reperfusion...
November 19, 2016: Transplant International: Official Journal of the European Society for Organ Transplantation
https://www.readbyqxmd.com/read/27835640/mass-spectrometry-based-metabolomics-comparison-of-liver-grafts-from-donors-after-circulatory-death-dcd-and-donors-after-brain-death-dbd-used-in-human-orthotopic-liver-transplantation
#9
Olga Hrydziuszko, M Thamara P R Perera, Richard Laing, Jennifer Kirwan, Michael A Silva, Douglas A Richards, Nick Murphy, Darius F Mirza, Mark R Viant
Use of marginal liver grafts, especially those from donors after circulatory death (DCD), has been considered as a solution to organ shortage. Inferior outcomes have been attributed to donor warm ischaemic damage in these DCD organs. Here we sought to profile the metabolic mechanisms underpinning donor warm ischaemia. Non-targeted Fourier transform ion cyclotron resonance (FT-ICR) mass spectrometry metabolomics was applied to biopsies of liver grafts from donors after brain death (DBD; n = 27) and DCD (n = 10), both during static cold storage (T1) as well as post-reperfusion (T2)...
2016: PloS One
https://www.readbyqxmd.com/read/27830292/slnr-is-a-positive-pathway-specific-regulator-for-salinomycin-biosynthesis-in-streptomyces-albus
#10
Zhenhong Zhu, Han Li, Pin Yu, Yuanyang Guo, Shuai Luo, Zhongbin Chen, Xuming Mao, Wenjun Guan, Yongquan Li
Salinomycin, a polyether antibiotic produced by Streptomyces albus, is widely used in animal husbandry as an anticoccidial drug and growth promoter. Situated within the salinomycin biosynthetic gene cluster, slnR encodes a LAL-family transcriptional regulator. The role of slnR in salinomycin production in S. albus was investigated by gene deletion, complementation, and overexpression. Gene replacement of slnR from S. albus chromosome results in almost loss of salinomycin production. Complementation of slnR restored salinomycin production, suggesting that SlnR is a positive regulator of salinomycin biosynthesis...
November 9, 2016: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/27829174/a-chimeric-protein-composed-of-numa-fused-to-the-dna-binding-domain-of-lana-is-sufficient-for-the-ori-p-dependent-dna-replication
#11
Eriko Ohsaki, Keiji Ueda
The Kaposi's sarcoma-associated herpesvirus (KSHV) genome is stably maintained in KSHV-infected PEL cell lines during cell division. We previously showed that accumulation of LANA in the nuclear matrix fraction could be important for the latent DNA replication, and that the functional significance of LANA should be its recruitment of ori-P to the nuclear matrix. Here, we investigated whether the forced localization of the LANA-DNA binding domain (DBD) to the nuclear matrix facilitated ori-P-containing plasmid replication...
November 6, 2016: Virology
https://www.readbyqxmd.com/read/27822302/do-post-trauma-symptoms-mediate-the-relation-between-neurobiological-stress-parameters-and-conduct-problems-in-girls
#12
Kimberly A Babel, Tijs Jambroes, Sanne Oostermeijer, Peter M van de Ven, Arne Popma, Robert R J M Vermeiren, Theo A H Doreleijers, Lucres M C Jansen
OBJECTIVE: Attenuated activity of stress-regulating systems has consistently been reported in boys with conduct problems. Results in studies of girls are inconsistent, which may result from the high prevalence of comorbid post-trauma symptoms. Therefore, the aim of the present study is to investigate post-trauma symptoms as a potential mediator in the relation between stress-regulation systems functioning and conduct problems in female adolescents. METHODS: The sample consisted of 78 female adolescents (mean age 15...
2016: Child and Adolescent Psychiatry and Mental Health
https://www.readbyqxmd.com/read/27791029/crystal-structure-of-the-dna-binding-domain-of-the-transcription-factor-t-bet-suggests-simultaneous-recognition-of-distant-genome-sites
#13
Ce Feng Liu, Gabriel S Brandt, Quyen Q Hoang, Natalia Naumova, Vanja Lazarevic, Eun Sook Hwang, Job Dekker, Laurie H Glimcher, Dagmar Ringe, Gregory A Petsko
The transcription factor T-bet (Tbox protein expressed in T cells) is one of the master regulators of both the innate and adaptive immune responses. It plays a central role in T-cell lineage commitment, where it controls the TH1 response, and in gene regulation in plasma B-cells and dendritic cells. T-bet is a member of the Tbox family of transcription factors; however, T-bet coordinately regulates the expression of many more genes than other Tbox proteins. A central unresolved question is how T-bet is able to simultaneously recognize distant Tbox binding sites, which may be located thousands of base pairs away...
October 25, 2016: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/27784581/a-novel-two-level-dielectric-barrier-discharge-reactor-for-methyl-orange-degradation
#14
Xumei Tao, Guowei Wang, Liang Huang, Qingguo Ye, Dongyan Xu
A novel pilot two-level dielectric barrier discharge (DBD) reactor has been proposed and applied for degradation of continuous model wastewater. The two-level DBD reactor was skillfully realized with high space utilization efficiency and large contact area between plasma and wastewater. Various conditions such as applied voltage, initial concentration and initial pH value on methyl orange (MO) model wastewater degradation were investigated. The results showed that the appropriate applied voltage was 13.4 kV; low initial concentration and low initial pH value were conducive for MO degradation...
October 23, 2016: Journal of Environmental Management
https://www.readbyqxmd.com/read/27779800/the-expanded-criteria-donor-for-kidney-transplant-not-a-nearly-new-car
#15
Johan De Meester
From late nineteen nineties, the higher number of suitable renal transplant candidates has forced the transplant community to re-explore the whole spectrum of deceased donors after brain death (DBD) as well as after cardiac death (DCD); in practice, donors of older age and donors with more chronic diseases or "medical complexities". This new kidney donor population - finally defined as extended criteria donors (ECD) - currently comprises on average 20 to 25%. In his seminal paper in 2003 on the introduction of ECD in the US, Metzger et al...
October 25, 2016: Transplant International: Official Journal of the European Society for Organ Transplantation
https://www.readbyqxmd.com/read/27762328/nuclear-receptor-nhr-48-is-required-for-pathogenicity-of-the-second-stage-j2-of-the-plant-parasite-meloidogyne-incognita
#16
Chao-Jun Lu, Bao-Yu Tian, Yi Cao, Cheng-Gang Zou, Ke-Qin Zhang
Nuclear receptors (NRs) are a diverse class of transcription factors, which are involved in regulating a large number of physiological events in metazoans. However, the function of NRs is poorly understood in plant-parasitic nematodes. Here, members of the NR1J+K group of NRs in nematodes, including the free-living and plant parasites, were examined and phylogenetically analyzed. We found that the number of members of the NR1J+K group in plant-parasitic nematodes was less than that in the free-living nematodes, suggesting this reduction of NR1J+K group members in plant parasites maybe arose during the separation of the free-living and intermediately plant parasitic nematodes (Bursaphelenchus xylophilus)...
October 20, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27753206/a-comparison-of-request-process-and-outcomes-in-donation-after-cardiac-death-and-donation-after-brain-death-results-from-a-national-study
#17
L A Siminoff, G P Alolod, M Wilson-Genderson, E Y N Yuen, H M Traino
Available literature points to healthcare providers' discomfort with donation after cardiac death (DCD) and their perception of public reluctance toward the procedure. Using a national sample, we report on the communication content of actual DCD and donation after brain death (DBD) approaches by organ procurement organization (OPO) requesters and compare family decision makers' (FDMs') experiences of both modalities. We recruited 1601 FDMs using a validated protocol; 347 (21.7%) were of potential DCD donors...
October 18, 2016: American Journal of Transplantation
https://www.readbyqxmd.com/read/27744197/effective-degradation-of-organic-water-pollutants-by-atmospheric-non-thermal-plasma-torch-and-analysis-of-degradation-process
#18
Avinash S Bansode, Supriya E More, Ejaz Ahmad Siddiqui, Shruti Satpute, Absar Ahmad, Sudha V Bhoraskar, Vikas L Mathe
The paper reports the use of atmospheric non-thermal plasma torch as a catalyst for degradation of various organic pollutants dissolved in water. A flow of He mixed with air was used to produce the dielectric barrier discharge (DBD), at the tip of the torch, using pulsed electric excitation at 12 kV. The torch, operated at a power of 750 mW/mm(2), was seen to completely degrade the aqueous solutions of the pollutants namely methylene blue (MB), methyl orange (MO) and rhodamine-B (RB), at around 10(-4) M concentrations, the concentration of polluants is one order higher than of routinely used heterogeneous photocatalytic reactions, within 10 min of irradiation time at room temperature...
October 13, 2016: Chemosphere
https://www.readbyqxmd.com/read/27742321/beneficial-effect-of-extracorporeal-membrane-oxygenation-on-organ-perfusion-during-management-of-the-unstable-brain-dead-donor-a%C3%A2-case-series
#19
J H Kang, B H Choi, K M Moon, Y M Park, K H Yang, J H Ryu, C W Chu
It is well known that the quality of organs retrieved from brain-dead donors (DBDs) is better than those retrieved from circulatory death donors. However, in situations of organ retrieval from marginal DBDs, who are unstable despite intensive care, transplantation outcomes are not good. Organ ischemia is the most important determining factor in decreased organ quality in circulatory death donors and in some DBDs. Extracorporeal membrane oxygenation (ECMO) for management of DBDs can be an emergency countermeasure...
September 2016: Transplantation Proceedings
https://www.readbyqxmd.com/read/27742320/organ-donation-and-transplantation-from-donors-with-systemic-infection-a-single-center-experience
#20
X Yuan, C Chen, J Zhou, M Han, X Wang, C Wang, X He
OBJECTIVES: Donors with bacteremia and sepsis are often considered to be controversial for organ retrieval due to potential transmission of an infectious agent to the recipient. Herein we report our initial experience of organ donation and transplantation results from donors with systemic infection. MATERIALS AND METHODS: From January 2013 to December 2014, 125 cases of donation were completed in our organ procurement organization including 90 cases of donation after brain death (DBD) and 35 cases of donation after circulatory death (DCD)...
September 2016: Transplantation Proceedings
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