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Co Culture Fermentation

Eoin Byrne, Krisztina Kovacs, Ed W J van Niel, Karin Willquist, Sven-Erik Svensson, Emma Kreuger
Background: Current EU directives demand increased use of renewable fuels in the transportation sector but restrict governmental support for production of biofuels produced from crops. The use of intercropped lucerne and wheat may comply with the directives. In the current study, the combination of ensiled lucerne ( Medicago sativa L.) and wheat straw as substrate for hydrogen and methane production was investigated. Steam-pretreated and enzymatically hydrolysed wheat straw [WSH, 76% of total chemical oxygen demand (COD)] and ensiled lucerne (LH, 24% of total COD) were used for sequential hydrogen production through dark fermentation and methane production through anaerobic digestion and directly for anaerobic digestion...
2018: Biotechnology for Biofuels
Kevin D'hoe, Stefan Vet, Karoline Faust, Frédéric Moens, Gwen Falony, Didier Gonze, Verónica Lloréns-Rico, Lendert Gelens, Jan Danckaert, Luc De Vuyst, Jeroen Raes
Whereas the composition of the human gut microbiome is well resolved, predictive understanding is still lacking. Here, we followed a bottom-up strategy to explore human gut community dynamics: we established a synthetic community composed of three representative human gut isolates ( Roseburia intestinalis L1-82, Faecalibacterium prausnitzii A2-165 and Blautia hydrogenotrophica S5a33) and explored their interactions under well-controlled conditions in vitro. Systematic mono- and pair-wise fermentation experiments confirmed competition for fructose and cross-feeding of formate...
October 16, 2018: ELife
Xiaohong Zhao, Xianwei Zou, Qian Li, Xu Cai, Liya Li, Jinren Wang, Yao Wang, Chen Fang, Fa Xu, Yun Huang, Benke Chen, Jintian Tang, Huiguo Wang
The inflammatory cellular model of RAW264.7 cells induced by lipopolysaccharide (LPS) has always been used to investigate the effect of anti-inflammatory agents in vitro. In the present study, the anti-inflammatory activity of total flavones extracted from the fermentation broth of the co-culture of Coprinus comatus and Morchella esculenta (MCF-F), and its potential molecular mechanism in LPS-challenged RAW264.7 macrophage cells were investigated. The data revealed that MCF-F exhibited anti-inflammatory activity in LPS-stimulated RAW264...
October 10, 2018: Microbial Pathogenesis
Kola Saheed Olorunnisola, Parveen Jamal, Md Zahangir Alam
Kinetic analysis of solid-state fermentation (SSF) of fruit peels with Phanerochaete chrysosporium and Schizophyllum commune mixed culture was studied in flask and 7 kg capacity reactor. Modified Monod kinetic model suggested by Haldane sufficiently described microbial growth with co-efficient of determination ( R 2 ) reaching 0.908 at increased substrate concentration than the classical Monod model ( R 2  = 0.932). Leudeking-Piret model adequately described product synthesis in non-growth-dependent manner ( R 2  = 0...
October 2018: 3 Biotech
F H Busanello, X Petridis, M V R So, R J B Dijkstra, S K Prashant, L W M van der Sluis
AIM: To investigate the influence of biofilm structure on the biofilm removal capacity of endodontic irrigants and to study changes in the architecture of the remaining biofilms. METHODOLOGY: Streptococcus oralis J22 and Actinomyces naeslundii T14V-J1 were co-cultured under different growth conditions on saliva-coated hydroxyapatite discs. A Constant Depth Film Fermenter (CDFF) was used to grow steady-state 4-day biofilms. Biofilms were grown under static conditions for 4 and 10 days within a confined space...
October 10, 2018: International Endodontic Journal
Valery Ripari, Yunpeng Bai, Michael G Gänzle
This work aimed to study the phenolic acid metabolism of sourdough lactic acid bacteria (LAB) in laboratory media, and in sourdough fermentation with single cultures and in co-fermentations. Lactobacilli were selected from isolates obtained from 35 sourdough samples. Isolates (114 strains) were screened for phenolic acid decarboxylase gene pdc and EPS production. Ferulic acid metabolism of the 18 pdc positive strains was evaluated in mMRS; all pcd positive strains converted ferulic acid by decarboxylation and/or reduction...
February 2019: Food Microbiology
Qiong Wu, Mi Ni, Kai Dou, Jun Tang, Jianhong Ren, Chuanjin Yu, Jie Chen
BACKGROUND: Bacillus spp. are a genus of biocontrol bacteria widely used for antibiosis, while Trichoderma spp. are biocontrol fungi that are abundantly explored. In this study, a liquid co-cultivation of these two organisms was tried firstly. RESULTS AND DISCUSSION: Through liquid chromatography-mass spectrometry/mass spectrometry (LC-MS/MS), it was discovered that with an inoculation in the ratio of 1.9:1, the antimicrobial effect of the co-cultured fermentation liquor of Bacillus amyloliquefaciens ACCC11060 and Trichoderma asperellum GDFS1009 was found to be significantly higher than that of pure-cultivation...
October 3, 2018: Microbial Cell Factories
Xiaoyu Peng, Weizhu Zeng, Sha Xu, Jian Zha, Jingwen Zhou, Guoqiang Xu
Both α-ketoglutaric acid (KGA) and pyruvic acid (PYR) are important keto acids. Efficient co-production of KGA and PYR has been achieved in our previous work, and could significantly decrease the cost of fermentation production. KGA and PYR have similar physical and chemical properties. Hence, finding a way to separate the two keto acids efficiently has become a key challenge. In this study, different chromatographic methods have been investigated, including ion-exchange chromatography, aluminum oxide chromatography and silica gel chromatography...
October 3, 2018: Biotechnology Progress
Shraddha Maitra, Atul Narang
Under aerobic conditions, Crabtree-negative yeasts grow but do not ferment, and under anaerobic conditions, they ferment but do not grow. It is therefore believed that fermentation by these yeasts is sensitive to small variations of the operating parameters, e.g. dilution rate D, mass transfer coefficient kla and oxygen solubility co*. However, this parametric sensitivity has never been quantified. Here, we present a method to quantify the parametric sensitivity of ethanol production in the Crabtree-negative yeast Scheffersomycesstipitis...
September 28, 2018: Microbiology
Svetlana Alexeeva, Jesús Adrián Guerra Martínez, Maciej Spus, Eddy J Smid
BACKGROUND: In complex microbial ecosystems such as the marine environment, the gastrointestinal tract, but also in mixed culture fermentations, bacteriophages are frequently found to be a part of the microbial community. Moreover, prophages or prophage-like elements are frequently identified in sequenced bacterial genomes. The mixed undefined starter cultures represent an ecosystem which is shaped by long term evolution under relatively defined environmental conditions and provides an interesting model to study co-evolution of phages and their hosts as well as the impact of diversity on microbial community stability...
September 24, 2018: BMC Microbiology
C Nobre, D A Gonçalves, J A Teixeira, L R Rodrigues
An integrated process enabling the simultaneous production and purification of fructo-oligosaccharides (FOS) was explored. A co-culture fermentation with Aspergillus ibericus (used as FOS producer strain) and Saccharomyces cerevisiae YIL162 W (for small saccharides removal) was optimized. Inoculation conditions of S. cerevisiae, fermentative broth composition, temperature and pH were optimized by experimental design. Yeast extract concentration and temperature were the most significant variables affecting FOS purity...
December 1, 2018: Carbohydrate Polymers
Neha Srivastava, Manish Srivastava, Vijai K Gupta, P W Ramteke, P K Mishra
Graphene oxide (GO) treated thermostable crude cellulase has been obtained via fungal co-cultivation of strain Cladosporium cladosporioides NS2 and Emericella variecolor NS3 using mix substrate of orange peel and rice straw under solid state fermentation (SSF). Enzyme activity of 60 IU/gds FP, 300 IU/gds EG and 400 IU/gds BGL are recorded in the presence of 1.0% GO in 96 h. This crude enzyme showed 50 °C as optimum incubation temperature, thermally stable at 55 °C for 600 min and stability in the pH range 4...
September 8, 2018: Bioresource Technology
Y Paola Maturano, M Victoria Mestre, Benjamín Kuchen, M Eugenia Toro, Laura A Mercado, Fabio Vazquez, Mariana Combina
Current consumer preferences are determined by well-structured, full-bodied wines with a rich flavor and with reduced alcohol levels. One of the strategies for obtaining wines with reduced ethanol content is sequential inoculation of non-Saccharomyces and Saccharomyces cerevisiae yeasts. However, different factors affect the production of metabolites like ethanol, glycerol and acetic acid by inoculated yeasts. In order to obtain low alcohol wines without quality loss, the aims of our study were: i) to determine optimum conditions (fermentation temperature and time of permanence and initial inoculum size of the non-Saccharomyces population at the beginning of the process, prior to inoculation with S...
August 18, 2018: International Journal of Food Microbiology
Maria J Mota, Rita P Lopes, Sérgio Sousa, Ana M Gomes, Ivonne Delgadillo, Jorge A Saraiva
Lactobacillus reuteri is a lactic acid bacterium able to produce several relevant bio-based compounds, including 1,3-propanediol (1,3-PDO), a compound used in food industry for a wide range of purposes. The performance of fermentations under high pressure (HP) is a novel strategy for stimulation of microbial growth and possible improvement of fermentation processes. Therefore, the present work intended to evaluate the effects of HP (10-35 MPa) on L. reuteri growth and glycerol/glucose co-fermentation, particularly on 1,3-PDO production...
November 2018: Food Research International
Anh T T Chau, Matthew Lee, Lorenz Adrian, Michael J Manefield
This study investigated syntrophic interactions between chlorinated benzene respiring Dehalococcoides mccartyi strain CBDB1 and fermenting partners ( Desulfovibrio vulgaris, Syntrophobacter fumaroxidans , and Geobacter lovleyi ) during hexachlorobenzene respiration. Dechlorination rates in syntrophic co-cultures were enhanced 2-3 fold compared to H2 fed CBDB1 pure cultures (0.23 ± 0.04 μmol Cl- day-1 ). Syntrophic partners were also able to supply cobalamins to CBDB1, albeit with 3-10 fold lower resultant dechlorination activity compared to cultures receiving exogenous cyanocobalamin...
2018: Frontiers in Microbiology
Van T Pham, Nicole Seifert, Nathalie Richard, Daniel Raederstorff, Robert Steinert, Kevin Prudence, M Hasan Mohajeri
The beneficial effects of prebiotic fibres on human health have been related to their capacities to alter the gut microbiota and modify the growth of beneficial microorganisms. It is long appreciated that bacterial metabolites affect the host's physiology. The inner lining of the intestinal tract is the first level of interaction between the host and bacteria and their metabolites. Therefore, we set out to test the effects of five common dietary fibres (oat β-glucan 28%; oat β-glucan 94%; dried chicory root containing inulin 75%; xylo-oligosaccharide; inulin 90%) and maltodextrin, after fermentation by human gut microbiota in vitro, on measures of gut barrier integrity using a Caco-2/HT29-MTX co-culture as well as mucus production and immune parameters using HT29-MTX and HT29 cell models, respectively...
2018: PeerJ
Paul Olusegun Bankole, Adedotun Adeyinka Adekunle, Sanjay Prabhu Govindwar
Efficient decolorization of cibracron brilliant red 3B-A dye by novel white rot fungal consortium was studied in static and shaking conditions using solid state fermentation technology. Daldinia concentrica (DC) and Xylaria polymorpha (XP) consortium showed dye removal efficiency than the individual strains within 5 days. The enzymes analysis revealed significant inductions in laccase (84%), lignin peroxidase (78%) and manganese peroxidase (65%) by the fungal co-culture (DC + XP), Xylaria polymorpha (XP) and Daldinia concentrica (DC) respectively...
December 2018: International Journal of Biological Macromolecules
Fu-Xing Niu, Xin He, Ya-Qin Wu, Jian-Zhong Liu
α-Pinene is a natural and active monoterpene, which is widely used as a flavoring agent and in fragrances, pharmaceuticals, and biofuels. Although it has been successfully produced by genetically engineered microorganisms, the production level of pinene is much lower than that of hemiterpene (isoprene) and sesquiterpenes (farnesene) to date. We first improved pinene tolerance to 2.0% and pinene production by adaptive laboratory evolution after atmospheric and room temperature plasma (ARTP) mutagenesis and overexpression of the efflux pump to obtain the pinene tolerant strain Escherichia coli YZFP, which is resistant to fosmidomycin...
2018: Frontiers in Microbiology
Mina Salehi, Ahmad Moieni, Naser Safaie
The microbial fermentation is considered as the potential source for large-scale production of paclitaxel. Since co-cultivation/mixed fermentation strategy has been reported as a yield enhancement strategy for paclitaxel production, investigation of fungal endophyte response to plant culture medium, plant cell extract (CE) and medium filtrate (MF) of plant cell suspension culture in terms of growth and paclitaxel production is interesting. In this study, 35 endophytic fungi were isolated from Taxus baccata and Corylus avellana grown in Iran...
August 13, 2018: Scientific Reports
Diego Moya, Alfred Ferret, Marta Blanch, Mari Carmen Fuentes, Jose Ignacio Fandiño, Sergio Calsamiglia
Live yeast additives may help optimize ruminal fermentation of high-grain diets, especially when the starch of the ration is highly fermentable. Eight dual flow continuous culture fermenters were used in a 2 × 2 factorial design in two replicated periods of 9 days (six for adaptation and three for sampling) to determine the effect of live yeast and type of cereal on rumen microbial fermentation and nutrient digestibility. Main factors were the addition of live yeast: no yeast (NY) vs. 2 × 107 CFU of yeast/g of diet (LY); and type of cereal in the diet: corn (CO) vs...
August 12, 2018: Journal of Animal Physiology and Animal Nutrition
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