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Co Culture Fermentation

Simon Portsmouth, David van Veenhuyzen, Roger Echols, Mitsuaki Machida, Juan Camilo Arjona Ferreira, Mari Ariyasu, Peter Tenke, Tsutae Den Nagata
BACKGROUND: Carbapenem-resistant Gram-negative bacteria represent the highest priority for addressing global antibiotic resistance. Cefiderocol (S-649266), a new siderophore cephalosporin, has broad activity against Enterobacteriaceae and non-fermenting bacteria, such as Pseudomonas aeruginosa and Acinetobacter baumannii, including carbapenem-resistant strains. We assessed the efficacy and safety of cefiderocol versus imipenem-cilastatin for the treatment of complicated urinary tract infection in patients at risk of multidrug-resistant Gram-negative infections...
December 2018: Lancet Infectious Diseases
Cristina Cattò, Elisa Garuglieri, Luigimaria Borruso, Daniela Erba, Maria Cristina Casiraghi, Francesca Cappitelli, Federica Villa, Sarah Zecchin, Raffaella Zanchi
Ingestion of silver nanoparticles (AgNPs) is inevitable linked to their widespread use in food, medicines and other consumer products. However, their effects on human microbiota at non-lethal concentrations remain poorly understood. In this study, the interactions among 1 μg mL-1 AgNPs, the intestinal microbiota, and the probiotic Bacillus subtilis (BS) were tested using in-vitro batch fermentation models inoculated with human fecal matter. Results from metagenomic investigations revealed that the core bacterial community was not affected by the exposure of AgNPs and BS at the later stage of fermentation, while the proportions of rare species changed drastically with the treatments...
November 12, 2018: Environmental Pollution
Kumari Vibha, Sangeeta Negi
The present study deals with the coproduction of laccase and pectinase enzymes through solid state fermentation using mixed fungal culture of Trametes hirsuta and Phanerochaete sp., to minimize the cost and time of the process. Substrates selected for the enzyme production were wheat bran, pulse husk and mustard peel. To get optimum yield of laccase and pectinase in a single fermenter, EVOP factorial design technique with factors like pH, incubation temperature and substrates ratio have been explored. At search level of EVOP, outcomes of the one factor at a time has been considered as incubation temperature 30 °C for 7 days at pH 5 with wheat bran: pulse husk: mustard peel ratio of 2:2:1 (w/w/w) and yield of laccase and pectinase was found as 78...
December 2018: 3 Biotech
Nikola Palevich, William J Kelly, Siva Ganesh, Jasna Rakonjac, Graeme T Attwood
Rumen bacterial species belonging to the genus Butyrivibrio are important degraders of plant polysaccharides, particularly hemicelluloses (arabinoxylans) and pectin. Currently, four species are recognized which have very similar substrate utilization profiles, but little is known about how these microorganisms are able to co-exist in the rumen. To investigate this question, Butyrivibrio hungatei MB2003 and Butyrivibrio proteoclasticus B316T were grown alone or in co-culture on xylan or pectin, and their growth, release of sugars, fermentation end products and transcriptomes were examined...
November 26, 2018: Applied and Environmental Microbiology
Mariem Theiri, Hassan Chadjaa, Mariya Marinova, Mario Jolicoeur
Butanol, a fuel with better characteristics than ethanol, can be produced via Acetone-Butanol-Ethanol (ABE) fermentation using lignocellulosic biomass as a carbon source. However, many inhibitors present in the hydrolysate limit the yield of the fermentation process. In this work, a detoxification technology combining flocculation and biodetoxification within a bacterial co-culture composed of Ureibacillus thermosphaericus and Cupriavidus taiwanensis is presented for the first time. Co-culture based strategies to detoxify filtered and unfiltered hydrolysates have been investigated...
November 23, 2018: Biotechnology Progress
Emmanuelle H Crost, Gwenaelle Le Gall, Jenny A Laverde-Gomez, Indrani Mukhopadhya, Harry J Flint, Nathalie Juge
Dietary and host glycans shape the composition of the human gut microbiota with keystone carbohydrate-degrading species playing a critical role in maintaining the structure and function of gut microbial communities. Here, we focused on two major human gut symbionts, the mucin-degrader Ruminococcus gnavus ATCC 29149, and R. bromii L2-63, a keystone species for the degradation of resistant starch (RS) in human colon. Using anaerobic individual and co-cultures of R. bromii and R. gnavus grown on mucin or starch as sole carbon source, we showed that starch degradation by R...
2018: Frontiers in Microbiology
Kuang-Hung Hsiao, Chun-Ming Huang, Yu-Hsiang Lee
Acne vulgaris, generally resulted from overgrowth of Propionibacterium acnes ( P. acnes ), is one of the most difficult-to-treat facial dermatoses and more than 90% of adolescents experienced the disease worldwide. Because the innate non-lymphoid immune system cannot effectively eliminate excessive P. acnes from the skin surface, so far the therapy of acne vulgaris is still mainly dependent on antibiotic treatment. However, long-term or overdose of antibiotics may initiate microbial drug resistance and/or generate unexpected side effects that seriously hamper the use of antibiotics in the clinic...
2018: Frontiers in Pharmacology
Liang Wang, Sean W York, Lonnie O Ingram, K T Shanmugam
Microorganisms ferment xylose at high rate only when glucose concentration in the medium falls below a critical level. Since the specific productivity of product is highest during exponential to early stationary phase of growth, a glucose utilization negative ethanologenic E. coli (strain LW419a) was constructed for high rate of xylose fermentation in combination with Turbo yeast. This co-culture fermented all the released sugars in an acid/enzyme-treated sugar cane bagasse slurry (10% solids) to an ethanol titer of 24...
November 7, 2018: Bioresource Technology
Emna El Golli-Bennour, Rim Timoumi, Meriam Koroit, Hassen Bacha, Salwa Abid-Essefi
Kefir is a fermented milk with numerous health favors counting restorative properties of bacterial flora, reduction of the symptoms of lactose intolerance, immune system stimulation, cholesterol reduction, as well as anti-mutagenic and anti-tumor properties. Zearalenone (ZEN) is a mycotoxin produced by some Fusarium species. ZEN often occurs as a contaminant in cereal grains and animal feeds. Human exposure occurs by ingestion of mycotoxin-contaminated products and can cause serious health problems. This study aimed to assess the preventive effect of kefir against ZEN toxicity in cultured HCT-116 colorectal carcinoma cells; by the evaluation of cell viability, oxidative stress status and the initiation of apoptotic cell death pathway...
November 16, 2018: Toxicon: Official Journal of the International Society on Toxinology
Saber Amiri, Reza Rezaei Mokarram, Mahmoud Sowti Khiabani, Mahmoud Rezazadeh Bari, Mohammad Alizadeh Khaledabad
In this study, the most important variables (incubation temperature, fermentation time and yeast extract concentration) responsible for the exopolysaccharides (EPSs) production by Lactobacillus acidophilus LA5 and Bifidobacterium animalis subsp. lactis BB12 were screened. The EPSs synthesize by LA5, BB12, and their co-culture were successfully optimized and were 349.82 ± 5.39, 146.83 ± 3.99 and 187.02 ± 1.54 mg/L, respectively. GC-MS analysis indicated that the purified EPSs are heteropolysaccharide and consisted of glucose, galactose, glucuronic acid, and xylose...
November 15, 2018: International Journal of Biological Macromolecules
Tong Pan, Hongyu Xiang, Taotao Diao, Wen Ma, Chao Shi, Yun Xu, Qiuhong Xie
The objective of this study was to develop a new type of feedstuff by utilizing probiotics to transform agricultural byproducts to meet the livestock feed shortage. In this study, peanut hull powder (PHP) was used as the fermentation substrate, which was inoculated with 18 kinds of feed probiotics combined with two kinds of nutritional additives. The desired value formula was used to select the strains suitable for fermentation of PHP, and the strains were combined as multi-culture starters. We compared the changes of the chemical composition and bacterial flora before and after the fermentation, which provided a theoretical basis for the establishment and quality evaluation of the system of PHP fermentation...
November 2, 2018: Bioresource Technology
Yafang Yu, Li Wang, Haifeng Qian, Hui Zhang, Yan Li, Gangcheng Wu, Xiguang Qi, Meijuan Xu, Zhiming Rao
The use of selected Saccharomyces cerevisiae PS7314, Lactobacillus rossiae NOS7307, Lactobacillus brevis NOS7311, and Lactobacillus plantarum NOS7315 as mono-culture or co-culture for production of sourdoughs, their breads showed different physical and organoleptic properties. The pH of breads fermented with sourdoughs incubated with mono-culture or co-culture all decreased. An opposite trend was found for TTA. The use of single lactobacillus for the dough fermentation decreased the specific volume of bread, which was 4...
March 15, 2019: Food Chemistry
M L Morales, J Fierro-Risco, R Ríos-Reina, C Ubeda, P Paneque
The aim of this work was to evaluate how the use of mixed cultures of Saccharomyces cerevisiae and Lachancea thermotolerans indigenous yeast strains influences the volatile composition of wine. Multivariate curve resolution (MCR) method has been applied to data analysis. Five fermentation trials were carried out: three co-inoculated with L. thermotolerans:S. cerevisiae, at the ratio of 50:1, 20:1 and 5:1 respectively, and two with a pure culture of each strain. A must from sun-dried Pedro Ximénez grapes was employed...
March 15, 2019: Food Chemistry
Edwina Romanens, Susette Freimüller Leischtfeld, Andrea Volland, Marc J A Stevens, Ursina Krähenmann, Désirée Isele, Birgit Fischer, Leo Meile, Susanne Miescher Schwenninger
Contamination with filamentous fungi during cocoa bean fermentation and drying reduces the quality of cocoa beans and poses a health risk for consumers due to the potential accumulation of mycotoxins. The aim of this study was to develop anti-fungal lactic acid bacteria (LAB)-yeast co-cultures by selecting anti-fungal strains best adapted to the cocoa bean fermentation process from 362 LAB and 384 yeast strains isolated from cocoa bean post-harvest processes. The applied multiphasic screening approach included anti-fungal activity tests in vitro and in vivo and assessment of the carbon metabolism and stress tolerance of the anti-fungal strains in a cocoa pulp simulation medium...
October 9, 2018: International Journal of Food Microbiology
Sathyanarayanan S Veeravalli, Alexander P Mathews
A novel low pH fermentation process was studied for the conversion of lactose using Lactobacillus plantarum and Lactobacillus buchneri under anoxic conditions in single co-culture, and two-stage sequential fermentations. This is aimed at producing acetate and propylene glycol (PG) as environmentally benign substitutes for currently used road and aircraft deicing chemicals. The results indicate that in the case of two-stage fermentation with immobilized L. buchneri in the second stage, lactose degradation rate increased markedly producing acetate and PG concentrations of 12...
January 2019: Enzyme and Microbial Technology
Kamil Charubin, Eleftherios Terry Papoutsakis
In microbial fermentations at least 33% of the sugar-substrate carbon is lost as CO2 during pyruvate decarboxylation to acetyl-CoA, with the corresponding electrons lost in the form of H2 . Previous attempts to reduce this carbon and electron loss focused on engineering of a single organism. In nature, most microorganisms live in complex communities where syntrophic interactions result in superior resource utilization. Here, we show that a synthetic syntrophy consisting of the solventogen Clostridium acetobutylicum, which converts simple and complex carbohydrates into a variety of chemicals, and the acetogen C...
November 2, 2018: Metabolic Engineering
Ling Leng, Masaru K Nobu, Takashi Narihiro, Peixian Yang, Giin-Yu Amy Tan, Po-Heng Lee
Glycerol is presently being generated in surplus with the rapid growth of the biodiesel industry and seeks ways to be upcycled, rather than to be treated with costs. Glycerol for the co-production of 1,3-propanediol (1,3-PDO) and caproate has a great prospect. Yet, its technical difficulty lies in the enhancement of caproate productivity, which requires the presence of ethanol as a co-substrate and necessitates the co-existence of functional microbes for glycerol fermentation and chain elongation. This study successfully achieved 6...
October 24, 2018: Water Research
Andrew J Sommer, Peter D Newell
Interactions between species shape the formation and function of microbial communities. In the gut microbiota of animals, cross-feeding of metabolites between microbes can enhance colonization and influence host physiology. We examined a mutually beneficial interaction between two bacteria isolated from the gut microbiota of Drosophila : Acetobacter fabarum and Lactobacillus brevis After developing an in vitro co-culture assay, we utilized a genetic screen to identify A. fabarum genes required for enhanced growth with L...
November 2, 2018: Applied and Environmental Microbiology
D Janßen, M A Ehrmann, R F Vogel
AIMS: In a previous study, we used a 5-day fermenting sausage model to characterize assertiveness of Lactobacillus curvatus and Lactobacillus sakei starter strains towards employ autochthonous contaminants. In this work, we probed those findings and their transferability to real sausage fermentation including the drying process in an industrial ring trial experiment. METHODS AND RESULTS: Raw fermented sausages ('salami') were produced with three L. curvatus and four L...
November 1, 2018: Journal of Applied Microbiology
Gyu-Sung Cho, Claudia Cappello, Katrin Schrader, Olakunle Fagbemigum, Folarin A Oguntoyinbo, Claudia Csovcsics, Niels Rösch, Jan Kabisch, Horst Neve, Wilhelm Bockelmann, Karlis Briviba, Monica Modesto, Elisabetta Cilli, Paola Mattarelli, Charles M A P Franz
The lactobacilli associated with a fermented goat milk product from Tajikistan were isolated to characterize their technological properties and antibiotic resistances in order to assess their suitability for development as starter cultures. In this study, twenty three strains were identified by 16S rRNA sequencing as typical dairy-associated lactic acid bacterial strains, i.e. L. plantarum , L. pentosus , L. delbrueckii , L. helveticus and L. paracasei strains. These strains were generally susceptible to most antibiotics tested in this study and this allowed a selection of strains as safe starters...
October 19, 2018: Journal of Microbiology and Biotechnology
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