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https://www.readbyqxmd.com/read/28821965/increased-endocytosis-of-magnetic-nanoparticles-into-cancerous-urothelial-cells-versus-normal-urothelial-cells
#1
Jasna Lojk, Vladimir Boštjan Bregar, Klemen Strojan, Samo Hudoklin, Peter Veranič, Mojca Pavlin, Mateja Erdani Kreft
The blood-urine barrier is the tightest and most impermeable barrier in the body and as such represents a problem for intravesical drug delivery applications. Differentiation-dependent low endocytotic rate of urothelial cells has already been noted; however, the differences in endocytosis of normal and cancer urothelial cells have not been exploited yet. Here we analysed the endocytosis of rhodamine B isothiocyanate-labelled polyacrylic acid-coated cobalt ferrite nanoparticles (NPs) in biomimetic urothelial in vitro models, i...
August 18, 2017: Histochemistry and Cell Biology
https://www.readbyqxmd.com/read/28821120/enhanced-permeability-and-antifouling-performance-of-cellulose-acetate-ultrafiltration-membrane-assisted-by-l-dopa-functionalized-halloysite-nanotubes
#2
REVIEW
Keguang Mu, Dalun Zhang, Ziqiang Shao, Dujian Qin, Yalong Wang, Shuo Wang
l-Dopa functionalized halloysite nanotubes (HNTs) were prepared by the self-polymerization of l-dopa in the weak alkaline condition. Then different contents of l-dopa coated HNTs (LPDHNTs) were blended into cellulose acetate to prepare enhanced performance ultrafiltration membranes via the phase inversion method. The HNTs and LPDHNTs were characterized by FTIR, XPS, and TEM anysis. And the membranes morphologies, separation performance, antifouling performance, mechanical properties and hydrophilicity were also investigated...
October 15, 2017: Carbohydrate Polymers
https://www.readbyqxmd.com/read/28821090/dyeing-and-characterization-of-regenerated-cellulose-nanofibers-with-vat-dyes
#3
Muzamil Khatri, Farooq Ahmed, Irfan Shaikh, Duy-Nam Phan, Qamar Khan, Zeeshan Khatri, Hoik Lee, Ick Soo Kim
Recent advancement in dyeing of nanofibers has been accelerated to improve their aesthetic properties, however, achieving good color fastness remains a challenge. Therefore, we attempt to improve the color fastness properties nanofibers. Vat dyes are known for better color fastness and their application on nanofibers has not been investigated to date. Herein, we report dyeing of regenerated cellulose nanofibers (RCNF) that were produced from precursor of cellulose acetate (CA) followed by deacetylation process...
October 15, 2017: Carbohydrate Polymers
https://www.readbyqxmd.com/read/28821050/clean-approach-to-synthesis-of-graphene-like-cufe2o4-polysaccharide-resin-nanohybrid-bifunctional-compound-for-dye-adsorption-and-bacterial-capturing
#4
Mostafa Hossein Beyki, Sarina Ehteshamzadeh Ganjbakhsh, Sara Minaeian, Farzaneh Shemirani
Focus of this research is on employment of a green route to prepare magnetic CuFe2O4@polysaccharide resin for environmental remediation purpose. The method is including solvent free solid state synthesis of copper ferrite nanoparticles by combustion route using cellulose as fuel. Then polysaccharide resin as well as its magnetic composite was prepared by using glucose, citric acid and copper ferrite as solid raw materials. Characterization study using FESEM and TEM images showed that as synthesized resin possesses graphene like structure as ferrite nanoparticles are dispersed in the matrice of resin...
October 15, 2017: Carbohydrate Polymers
https://www.readbyqxmd.com/read/28821045/production-of-cellulose-nanofibers-using-phenolic-enhanced-surface-oxidation
#5
Iman Beheshti Tabar, Ximing Zhang, Jeffrey P Youngblood, Nathan S Mosier
In this study we demonstrate that lignin monomers formed as byproducts of pulping or bioprocessing of lignocellulosic biomass is an effective enhancer to oxidize cellulose surfaces with ozone for the production of cellulose nanofibers (CNF). Never dried softwood pulp with minimum mercerization was enzymatically treated leading to a homogeneous pulp slurry with a higher reactivity. The slurry was oxidized by ozone gas in the presence of syringic acid, a lignin degradation model compound, as an oxidation enhancer at room temperature and pH 11...
October 15, 2017: Carbohydrate Polymers
https://www.readbyqxmd.com/read/28820997/nh2fe3o4-sio2-supported-peroxidase-catalyzed-h2o2-for-degradation-of-endocrine-disrupter-from-aqueous-solution-roles-of-active-radicals-and-noms
#6
Jing Ai, Weijun Zhang, Guiying Liao, Hua Xia, Dongsheng Wang
In this work, magnetic Fe3O4 was utilized to immobilize horseradish peroxidase (IM-HRP) in order to improve its stability and reusability by crosslinking method process with glutaraldehyde. The physicochemical properties of NH2Fe3O4@SiO2 and IM-HRP were characterized by powder X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FT-IR), Thermo-gravimetric Analysis (TGA) and Transmission electron microscopy (TEM). The thermal stability of immobilized-HRP was considerably improved in comparison with free counterpart...
August 13, 2017: Chemosphere
https://www.readbyqxmd.com/read/28819966/characterization-of-nanopipet-supported-ities-tips-for-scanning-electrochemical-microscopy-of-single-solid-state-nanopores
#7
Ran Chen, Ryan James Balla, Alex Lima, Shigeru Amemiya
Nanoscale scanning electrochemical microscopy (SECM) is a powerful scanning probe technique that enables high-resolution imaging of chemical processes at single nanometer-sized objects. However, it has been a challenging task to quantitatively understand nanoscale SECM images, which requires to accurately characterize the size and geometry of nanoelectrode tips. Herein, we address this challenge through transmission electron microscopy (TEM) of quartz nanopipets for SECM imaging of single solid-state nanopores by using nanopipet-supported interfaces between two immiscible electrolyte solutions (ITIES) as tips...
August 18, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28819929/a-comparative-study-of-the-adhesion-of-biosynthesized-gold-and-conjugated-gold-prodigiosin-nanoparticles-to-triple-negative-breast-cancer-cells
#8
S O Dozie-Nwachukwu, J D Obayemi, Y Danyuo, N Anuku, O S Odusanya, K Malatesta, W O Soboyejo
This paper explores the adhesion of biosynthesized gold nanoparticles (AuNPs) and gold (Au) nanoparticle/prodigiosin (PG) drug nanoparticles to breast cancer cells (MDA-MB-231 cells). The AuNPs were synthesized in a record time (less than 30 s) from Nauclea latifolia leaf extracts, while the PG was produced via bacterial synthesis with Serratia marcescens sp. The size distributions and shapes of the resulting AuNPs were characterized using transmission electron microscopy (TEM), while the resulting hydrodynamic diameters and polydispersity indices were studied using dynamic light scattering (DLS)...
August 17, 2017: Journal of Materials Science. Materials in Medicine
https://www.readbyqxmd.com/read/28819905/adsorption-properties-of-kaolinite-based-nanocomposites-for-fe-and-mn-pollutants-from-aqueous-solutions-and-raw-ground-water-kinetics-and-equilibrium-studies
#9
Mohamed Shaban, Mohamed E M Hassouna, Fadya M Nasief, Mostafa R AbuKhadra
Raw kaolinite was used in the synthesis of metakaolinite/carbon nanotubes (K/CNTs) and kaolinite/starch (K/starch) nanocomposites. Raw kaolinite and the synthetic composites were characterized using XRD, SEM, and TEM techniques. The synthetic composites were used as adsorbents for Fe and Mn ions from aqueous solutions and natural underground water. The adsorption by the both composites is highly pH dependent and achieves high efficiency within the neutral pH range. The experimental adsorption data for the uptake of Fe and Mn ions by K/CNTs were found to be well represented by the pseudo-second-order kinetic model rather than the intra-particle diffusion model or Elovich model...
August 17, 2017: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/28819665/efficient-photoelectrochemical-water-splitting-on-ultrasmall-defect-rich-taox-nanoclusters-enhanced-by-size-selected-pt-nanocluster-promoters
#10
Saurabh Srivastava, Joseph Palathinkal Thomas, Nina Heinig, Marwa Abd-Ellah, Md Anisur Rahman, Kam Tong Leung
Formation of nanoclusters has attracted a lot of attention in recent years because of their distinct properties from isolated atoms and bulk solids. Here, we focus on the catalytic properties of supported transition metal oxide nanoclusters, such as TaO2, with a well-defined size distribution below 10 nm. We show that their catalytic performance can be greatly enhanced by introducing a reaction promoter such as Pt. Different combinations of precisely size-selected, defect-rich TaOx and Pt nanoclusters are produced by a gas-phase aggregation technique in a special DC magnetron sputtering system...
August 18, 2017: Nanoscale
https://www.readbyqxmd.com/read/28819550/effects-of-storage-temperature-on-airway-exosome-integrity-for-diagnostic-and-functional-analyses
#11
Rosario Maroto, Yingxin Zhao, Mohammad Jamaluddin, Vsevolod L Popov, Hongwang Wang, Madumali Kalubowilage, Yueqing Zhang, Jonathan Luisi, Hong Sun, Christopher T Culbertson, Stefan H Bossmann, Massoud Motamedi, Allan R Brasier
Background: Extracellular vesicles contain biological molecules specified by cell-type of origin and modified by microenvironmental changes. To conduct reproducible studies on exosome content and function, storage conditions need to have minimal impact on airway exosome integrity. Aim: We compared surface properties and protein content of airway exosomes that had been freshly isolated vs. those that had been treated with cold storage or freezing. Methods: Mouse bronchoalveolar lavage fluid (BALF) exosomes purified by differential ultracentrifugation were analysed immediately or stored at +4°C or -80°C...
2017: Journal of Extracellular Vesicles
https://www.readbyqxmd.com/read/28819151/the-investigation-of-stress-in-freestanding-gan-crystals-grown-from-si-substrates-by-hvpe
#12
Moonsang Lee, Dmitry Mikulik, Mino Yang, Sungsoo Park
We investigate the stress evolution of 400 µm-thick freestanding GaN crystals grown from Si substrates by hydride vapour phase epitaxy (HVPE) and the in situ removal of Si substrates. The stress generated in growing GaN can be tuned by varying the thickness of the MOCVD AlGaN/AlN buffer layers. Micro Raman analysis shows the presence of slight tensile stress in the freestanding GaN crystals and no stress accumulation in HVPE GaN layers during the growth. Additionally, it is demonstrated that the residual tensile stress in HVPE GaN is caused only by elastic stress arising from the crystal quality difference between Ga- and N-face GaN...
August 17, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28819120/dual-delivery-of-fgf-2-ctgf-from-silk-fibroin-plcl-peo-coaxial-fibers-enhances-msc-proliferation-and-fibrogenesis
#13
Ruodan Xu, Huiling Zhao, Hanif Muhammad, Mingdong Dong, Flemming Besenbacher, Menglin Chen
The success of mesenchymal stem cell transplantation is highly dependent on their survival and controlled fate regulation. This study demonstrates that dual-delivery of connective tissue growth factor (CTGF) and fibroblast growth factor 2 (FGF-2) from a core-shell fiber of Silk Fibroin/poly(L-lactic acid-co-ε-caprolactone)-polyethylene oxide (SF/PLCL-PEO) enhanced fibrogenic lineage differentiation of MSCs. The core-shell structure was confirmed by transmission electron microscopy (TEM), fluorescence microscopy and attenuated total reflection (ATR) Fourier transform infrared (FTIR) spectroscopy...
August 17, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28819087/investigation-and-characterization-of-%C3%AE-lactam-resistance-in-escherichia-coli-strains-isolated-from-bamboo-rats-rhizomys-sinensis-in-zhejiang-province-china
#14
Hui Zhang, Kun Li, Yajing Wang, Mujeeb Ur Rehman, Yijiang Liu, Junjie Jin, Junping Peng, Fazul Nabi, Khalid Mehmood, Houqiang Luo, Jiaxiang Wang
This study was undertaken to investigate drug resistance in Escherichia coli (E. coli) strains isolated from bamboo rats in Zhejiang province of China. One hundred and fifty-four E. coli strains were isolated from dead bamboo rats. Polymerase chain reaction (PCR) was used to detect the representative genes encoding resistance to commonly used β-lactam antibiotics. Highest resistance was observed for cefradine (24.03%), followed by penicillin (20.78%) and ceftazidime (20.13%). The isolation rates of β-lactam resistance genes were 53...
August 17, 2017: Journal of Veterinary Medical Science
https://www.readbyqxmd.com/read/28818776/bacillus-thuringiensis-coated-zinc-oxide-nanoparticle-and-its-biopesticidal-effects-on-the-pulse-beetle-callosobruchus-maculatus
#15
Balasubramanian Malaikozhundan, Baskaralingam Vaseeharan, Sekar Vijayakumar, Merlin P Thangaraj
Insect pests belonging to the genus Callosobruchus are the major cause of damage to stored pulse crops. Recently, nanotechnology has emerged as a promising tool for pest control. In the present study, we report for the first time the synthesis and biological evaluation of Bacillus thuringiensis coated zinc oxide nanoparticles (Bt-ZnO NPs) on the pulse beetle, Callosobruchus maculatus. The biologically synthesized Bt-ZnO NPs were extensively characterized using UV-Vis spectroscopy, X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), Transmission electron microscopy (TEM) and Zeta potential...
August 9, 2017: Journal of Photochemistry and Photobiology. B, Biology
https://www.readbyqxmd.com/read/28818722/interaction-of-manganese-nanoparticle-with-cytochrome-c-a-multi-spectroscopic-study
#16
Anali Mansouri, Mina Mousavi, Farnoosh Attar, Ali Akbar Saboury, Mojtaba Falahati
In this paper, the conformational changes of cytochrome c (cyt c) upon interaction with manganese nanoparticle (Mn-NP) were examined using dynamic light scattering (DLS), transmission electron microscopy (TEM), Fourier transform infrared spectroscopy (FTIR), zeta potential, fluorescence spectroscopy, and circular dichroism (CD) spectroscopy methods DLS and TEM analysis exhibited the structure of Mn-NP was less than 50nm. FTIR bands were similar to those reported for Mn-NP. Zeta potential measurements showed positive charge distribution for Mn-NP (4...
August 14, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28818719/green-approach-for-one-pot-synthesis-of-silver-nanorod-using-cellulose-nanocrystal-and-their-cytotoxicity-and-antibacterial-assessment
#17
Th I Shaheen, Amr Fouda
Herein, this research addresses an innovative approach for one-pot synthesis of highly stabilized silver nanorods in powder form at concentration as high as feasible to be proposed in large-scale production via cellulose nanocrystals (CNC). For the first time, CNC without any surface modification in the presence of alkali is acting as both reducing and stabilizing agent for assembling of Ag nanorods. Extraction of CNC from cotton is carried out as per to acid hydrolysis technique. Thorough assessments of Ag nanorods formation, structural and morphological characteristics of Ag nanorods were investigated by making use of UV-vis spectroscopy, TEM, DLS, AFM and X-ray diffraction(XRD) analysis...
August 14, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28818314/comparison-of-disaggregative-effect-of-a-type-egcg-dimer-and-egcg-monomer-on-the-preformed-bovine-insulin-amyloid-fibrils
#18
Rong-Zu Nie, Wei Zhu, Jin-Ming Peng, Zhen-Zhen Ge, Chun-Mei Li
In the present study, the disruptive effects of epigallocatechin-3-gallate (EGCG) and A-type dimeric epigallocatechin-3-gallate (A-type EGCG dimer) on the preformed bovine insulin amyloid fibrils were studied by several biophysical methods including thioflavin-T (ThT) fluorescence assay, 1-anilinonaphthalene-8-sulfonic (ANS) fluorescence assay, Congo red (CR) binding assay, dynamic light scattering (DLS), transmission electron microscopy (TEM), Gel electrophoresis (SDS-PAGE) and Bradford assay. Our results demonstrated that A-type EGCG dimer showed significantly more potential disaggregative effects on the bovine insulin amyloid fibrils than EGCG...
July 31, 2017: Biophysical Chemistry
https://www.readbyqxmd.com/read/28817106/ag-coated-heterostructures-of-zno-tio%C3%A2-delaminated-montmorillonite-as-solar-photocatalysts
#19
Carolina Belver, Mariana Hinojosa, Jorge Bedia, Montserrat Tobajas, Maria Ariadna Alvarez, Vicente Rodríguez-González, Juan Jose Rodriguez
Heterostructures based on ZnO-TiO₂/delaminated montmorillonite coated with Ag have been prepared by sol-gel and photoreduction procedures, varying the Ag and ZnO contents. They have been thoroughly characterized by XRD, WDXRF, UV-Vis, and XPS spectroscopies, and N₂ adsorption, SEM, and TEM. In all cases, the montmorillonite was effectively delaminated with the formation of TiO₂ anatase particles anchored on the clay layer's surface, yielding porous materials with high surface areas. The structural and textural properties of the heterostructures synthesized were unaffected by the ZnO incorporated...
August 17, 2017: Materials
https://www.readbyqxmd.com/read/28816383/the-structure-and-ultrastructure-of-the-egg-capsules-of-stoneflies-of-the-genus-isoperla-insecta-plecoptera-perlodidae
#20
Anna Michalik, Marko Miliša, Katarzyna Michalik, Elżbieta Rościszewska
The egg capsules of five systellognathan stoneflies species representing the genus Isoperla (Plecoptera, Perlodidae) have been investigated using light and electron microscopes (SEM and TEM). We consider the structural modifications of egg coverings (egg capsules, eggshells) like: a shape of a capsule, presence of structures fixing an egg to the substratum under water, intrachorionic aeropylar system facilitating gas exchange as a factor adapting an egg/embryo to the environment. The structures protecting eggs against desiccation during a female flight before oviposition into water as well as against the dangers of external mechanical injury caused by turbulences in rapid water currents of mountain streams are described and discussed...
August 17, 2017: Microscopy Research and Technique
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