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"Chitosan Nanoparticle"

Shirin Heidarisasan, Nasrin Ziamajidi, Jamshid Karimi, Roghayeh Abbasalipourkabir
Objectives: Chronic hyperglycemia leads to activation of the advanced glycation end products (AGE)-receptor (RAGE) for AGE axis and oxidative stress, which promote diabetic renal damage. This study examines the effect of insulin-loaded trimethyl chitosan nanoparticles on the kidney tissue of diabetic rats. Materials and Methods: Twenty-five male Wistar rats were randomly divided into 5 groups: normal control (C), diabetic group without treatment (DM), diabetic group treated with chitosan-based nanoparticle (DM+NP, 1 ml by gavage), diabetic group treated with 8 IU/kg insulin-loaded trimethyl chitosan nanoparticles (DM+N...
October 2018: Iranian Journal of Basic Medical Sciences
Ana Isabel Barbosa, Sofia A Costa Lima, Salette Reis
In this study, fucoidan/chitosan nanoparticles were developed for the topical delivery of methotrexate towards the treatment of skin-related inflammatory diseases. Based on the fucoidan/chitosan (F/C) weight ratio, three different nanoparticles (1F/1C; 3F/1C; 5F/1C) were produced and characterized. Methotrexate was loaded in these polymeric nanoparticles achieving a drug loading of ca. 14% and an entrapment efficiency of 96, 87 and 80% for 1F/1C; 3F/1C and 5F/1C nanoparticles, respectively. Methotrexate-loaded fucoidan/chitosan nanoparticles exhibited size within the 300-500 nm range, positive zeta potential for 1F/1C nanoparticles (+60 mV) and negative surface charge for the 3F/1C and 5F/1C nanoparticles (-40 and -45 mV, respectively)...
December 3, 2018: International Journal of Biological Macromolecules
Nida Mubin, Mohd Saad Umar, Swaleha Zubair, Mohammad Owais
In the present study, we investigated potential of chitosan-based nanoparticles (CNPs) to deliver loaded therapeutic molecules to pathogen harboring macrophages. We fabricated stable CNPs employing ionic cross-linking method and evaluated their potential to target RAW 264.7 cells. The physicochemical characterization of as-synthesized CNPs was determined using electron microscopy, infrared microscopy and zeta potential measurement. Next, cellular uptake and intracellular localization studies of CNPs were followed in living RAW264...
2018: Frontiers in Microbiology
Steffen Sydow, Dominik de Cassan, Robert Hänsch, Thomas R Gengenbach, Christopher D Easton, Helmut Thissen, Henning Menzel
Nanogels were prepared by ionotropic gelation of chitosan (CS) with tripolyphosphate (TPP). The use of such nanogels to prepare coatings by layer-by-layer deposition (LbL) was studied. The nanogels were characterized in terms of particle size, zeta-potential and stability. Nanogel suspensions were used to build polyelectrolyte multilayers on silicon wafers and on PCL fiber mats by LbL-deposition. Three different polysaccharides were used as polyanions, namely chondroitin sulfate, alginate and hyaluronic acid...
December 4, 2018: Biomaterials Science
Sylvie Skalickova, Michaela Docekalova, Martina Stankova, Dagmar Uhlirova, Branislav Ruttkay-Nedecky, Carlos Fernandez, Marta Kepinska, Halina Milnerowicz, Rene Kizek
Modern anticancer therapy aims to increase the effectiveness of tumor treatment. The aim of this work was to propose a new nanotransporter for targeted delivery of anthracycline antibiotics, which is characterized by its bioavailability, increased uptake of the drug from the bloodstream at the site of the tumor tissue as well as low toxicity to non-target tissue. Chitosan nanoparticles have attracted great attention in the field of drug delivery due to their stability, low toxicity and easy preparation. Deacetylated chitosan skeleton is composed of glucosamine units and has a high density of charged amino groups which allow strong electrostatic interactions with biomolecules, transition metals (Zn, Se) and peptides...
May 1, 2019: Journal of Nanoscience and Nanotechnology
G Deepa, K C Sivakumar, T P Sajeevan
The present work is an attempt to integrate the molecular simulation studies with in vitro cytotoxicity of cytarabine-loaded chitosan nanoparticles and exploring the potential of this formulation as therapeutics for treating solid tumours. The molecular simulation was performed using GROMACS v5.4 in which, chitosan polymer (CHT; six molecules) was used to study the encapsulation and release of a single molecule of cytarabine. Root Mean Square Deviation (RMSD) of the Cα atom of cytarabine (CBR) molecule shows that CBR starts to diffuse out of the CHT polymer binding pocket around 10...
December 2018: 3 Biotech
Bo Qiu, Xiong-Feng Xu, Rong-Hui Deng, Gan-Qing Xia, Xi-Fu Shang, Pang-Hu Zhou
Osteoarthritis (OA) is a common degenerative joint disease characterized by inflammation of synoviocytes and degradation of cartilage. In the present study, hyaluronic acid/chitosan (HA/CS) nanoparticles were used as a vehicle for gene therapy of OA, and the cytokine response modifier A (CrmA) pDNA was proposed as the target gene. The HA/CS/pCrmA nanoparticles were prepared and the characteristics of the nanoparticles were examined. The nanoparticles were spherical, and the smallest size was obtained with the HA:CS weight ratio of 1:4...
November 26, 2018: International Journal of Molecular Medicine
Mireya Esbeiddy Chávez-Magdaleno, Ramsés Ramón González-Estrada, Anelsy Ramos-Guerrero, Maribel Plascencia-Jatomea, Porfirio Gutiérrez-Martínez
Preventive and curative activity of postharvest treatments with chitosan nanoparticles (CS) and chitosan biocomposites loaded with pepper tree essential oil (CS-PEO) against anthracnose were evaluated on Avocado ( Persea americana ) cv. Hass artificially inoculated in rind wounds. After 10 days of storage significant preventive and curative activity against Colletotrichum gloeosporioides was observed with the absence of internal damage by applying CS and CS-PEO. Quality parameters like water losses and firmness changes were assessed on fruit treated...
December 2018: Food Science and Biotechnology
Andre Correa Amaral, Pedro H V Saavedra, Ana Camila Oliveira Souza, Maryanne Trafani de Melo, Antonio Claudio Tedesco, Paulo Cesar Morais, Maria Sueli Soares Felipe, Anamelia Lorenzetti Bocca
OBJECTIVES: In this study, polymeric nanoparticles based on chitosan incorporating the antifungal miconazole nitrate were fabricated and testedin vivo using murine vulvovaginal candidiasis. METHODS: Nanoparticles prepared by the ionotropic gelation method presented 200 to 300 nm diameter and polydispersity indexes ranging from 0.2 to 0.4. The nanoparticles were prepared to incorporate 63.9 mg/mL of miconazole nitrate to be testedin vivo. Murine vulvovaginal candidiasis was standardized using estradiol valerate before the animals were challenged by Candida albicans...
November 20, 2018: Colloids and Surfaces. B, Biointerfaces
Jorge Gracida, Teresita Arredondo-Ochoa, Blanca E García-Almendárez, Monserrat Escamilla-García, Keiko Shirai, Carlos Regalado, Aldo Amaro-Reyes
Enzymes are gradually increasingly preferred over chemical processes, but commercial enzyme applications remain limited due to their low stability and low product recovery, so the application of an immobilization technique is required for repeated use. The aims of this work were to produce stable enzyme complexes of cross-linked xylanase on magnetic chitosan, to describe some characteristics of these complexes, and to evaluate the thermal stability of the immobilized enzyme and its reusability. A xylanase was cross-linked to magnetite particles prepared by in situ co-precipitation of iron salts in a chitosan template...
November 27, 2018: Applied Biochemistry and Biotechnology
K Divya, Smitha Vijayan, Sreekumar Janardanan Nair, M S Jisha
The worldwide rice production has been dwindling due to biotic and abiotic causes. Chitosan is a proven biofunctional material that induces many biological responses in plants. However, the growth and yield increasing properties of chitosan nanoparticles (ChNP) on rice crop are not well understood. In the present work, effect of ChNP on germination of rice has been studied. Seed toxicity of ChNP was also analyzed to ensure the safety of ChNP application. The toxicity study was done according to EPA guidelines and ChNP was found to be non-toxic...
November 23, 2018: International Journal of Biological Macromolecules
Juan Chen, Xiaobing Yang, Liuqing Huang, Huixian Lai, Chuanhai Gan, Xuetao Luo
Combination chemotherapy is widely exploited for suppressing drug resistance and achieving synergistic anticancer efficacy in the clinic. In this paper, the nanostructured targeting methotrexate (MTX) plus pemetrexed (PMX) chitosan nanoparticles (CNPs) were developed by modifying methoxy polye (thylene glycol) (mPEG), in which PEGylation CNPs was used as stealth nanocarriers (PCNPs) and MTX was employed as a targeting ligand and chemotherapeutic agent as well. Studies were undertaken on human lung adenocarcinoma epithelial (A549) and Lewis lung carcinoma (LLC) cell lines, revealing the anti-tumor efficacy of nanoparticle drug delivery system...
November 2018: Drug Delivery
Raghda Hassan, Salma N Tammam, Sara El Safy, Mohammad Abdel-Halim, Anastasia Asimakopoulou, Ralf Weiskirchen, Samar Mansour
Preventing hepatic stellate cell (HSC) activation represents a promising approach to resolve liver fibrosis. Several drugs have been reported to delay/prevent HSCs activation, however with limited clinical benefits. The latter may be in part attributed to the limited ability of such drugs in targeting more than one pathway of HSC activation. Added to that, is their inability of reaching their target cell in sufficient amounts to induce a therapeutic effect. In this work, chitosan NPs were loaded with JQ1 and atorvastatin, two drugs that have been reported to prevent HSCs activation, however via different mechanisms...
November 21, 2018: European Journal of Pharmaceutics and Biopharmaceutics
Doğa Kavaz, Maryam Idris, Confidence Onyebuchi
Essential oil of Cyperus articulatus have known bioactivities, the qualitative chemical composition analysis of the Essential Oils (EOs) by Gas Chromatography-Mass Spectrometry (GC-MS) revealed the presence of new compounds not previously known to the plant rhizome which includes, sesquiterpenes, monoterpenes, nootkatone, 6-methyl-3,5-heptadien-2-one, retinene, nopinone, cycloeucalenol, anozol, toosendanin, furanone, ethanone and vitamin A. Cyperus articulatus Essential Oils (CPEOs) loaded Chitosan Nanoparticles (CSNPs) was successful synthesized using an oil-in-water mixture and ionic gelation method and were characterized by Scanning Electron Microscopy (SEM), Fourier Transform Infrared Spectroscopy (FTIR) and UV-Vis spectrophotometry...
November 19, 2018: International Journal of Biological Macromolecules
Palanirajan Vijayaraj Kumar, Marwan Abdelmahmoud Abdelkarim Maki, Yeong Siew Wei, Lee Ming Tatt, Manogaran Elumalai, Shiau-Chuen Cheah, Bharathy Raghavan, Abu Bakar Bin Abdul Majeed
BACKGROUND: Recombinant human keratinocyte growth factor (rHuKGF) has gained considerable attention by researchers as epithelial cells proliferating agent. Moreover, intravenous truncated rHuKGF (palifermin) has been approved by Food and Drug Administration (FDA) to treat and prevent chemotherapy-induced oral mucositis and small intestine ulceration. The labile structure and short circulation time of rHuKGF in-vivo are the main obstacles that reduce the oral bioactivity and dosage of such proteins at the target site...
November 19, 2018: Current Clinical Pharmacology
M E Abd El-Aziz, S M M Morsi, Dina M Salama, M S Abdel-Aziz, Mohamed S Abd Elwahed, E A Shaaban, A M Youssef
Chitosan nanoparticles (CS-NPs) and chitosan/polyacrylic acid hydrogel nanoparticles (CS/PAA-HNPs) were obtained by ionic gelation with tripolyphosphate anions and copolymerization of CS with acrylic acid (AA), respectively. The prepared NPs were loaded by different concentrations of copper (1, 2 and 3% with respect to CS) to obtain chitosan/copper nanocomposites (CS/Cu-NCs) and chitosan/polyacrylic acid/copper hydrogel nanocomposites (CS/PAA/Cu-HNCs). The prepared NPs and their NCs were characterized by different techniques...
November 16, 2018: International Journal of Biological Macromolecules
Amna Shahzad, Ahmad Khan, Zakia Afzal, Muhammad Farooq Umer, Jallat Khan, Gul Majid Khan
The present study focuses on the preparation of nanoparticles-loaded ionic cross-linked films for the topical delivery of cefazolin. The aim of the study was to prepare a dosage form which can provide local effect of cefazolin along with sustained delivery at the site of application. Cefazolin was loaded into chitosan nanoparticles to mask the burst release of the drug and they were optimized based on particle size, PDI, % EE and zeta potential. Finally, the prepared nanoparticles were loaded into the films comprising of sodium alginate and pectin which were then subjected to cross-linking via calcium chloride to improve the mechanical strength of the hydrogel films upon exposure to wound fluid...
November 15, 2018: International Journal of Biological Macromolecules
Xuan Wei, Jianhong Liao, Zahra Davoudi, Hua Zheng, Jingru Chen, Dan Li, Xiong Xiong, Yihua Yin, Xiuxiang Yu, Jinghui Xiong, Qun Wang
For enhanced intracellular accumulation of 6-mercaptopurine (6-MP) in leukemia, a folate receptor-targeted and glutathione (GSH)-responsive polymeric prodrug nanoparticle was made. The nanoparticles were prepared by conjugating 6-MP to carboxymethyl chitosan via a GSH-sensitive carbonyl vinyl sulfide linkage, ultrasonic self-assembly and surface decoration with folate. The TEM graphs shows that the as-synthesized nanoparticles are spherical with a particle size of 170~220 nm. In vitro drug release of nanoparticles demonstrated acceptable stability in PBS containing 20 μM GSH at pH 7...
November 8, 2018: Marine Drugs
Yue Li, Yan Lin, Chun Liu Fu, Jiawei Tu, Chaopin Yang, Zhi Yi Chen
Objective-To study transfection efficiency of folate-modified chitosan (FA-CS) nanoparticles as a non-viral vector delivering pEGFP-C3plasmid (FA-CS/P) to 293T cells with or without the combination of ultrasound and microbubble. Method-pEGFP-C3 was used as reporter gene and FA-CS nanoparticles, which prepared by complex coagulation method were used as biological carriers. Transfection efficiency to 293T cells mediated by FA-CS/P nanoparticles, ultrasound (US) and microbubble (MB) was assessed by fluorescence microscopy and flow cytometry...
November 9, 2018: Current Molecular Medicine
Juan-Juan Ma, Yi-Gang Yu, Shou-Wei Yin, Chuan-He Tang, Xiaoquan Yang
In this work, zein/chitosan nanoparticles (ZCPs-Q) were developed for encapsulating quercetin to overcome its lower water solubility and instability, and to concomitantly enhance its cellular uptake and intracellular antioxidant activity. This strategy enhanced quercetin solubility 753.6 and 9.95 times in water and PBS (7.4), respectively, and quercetin encapsulated in ZCPs remained stable after UV irradiation and heat treatment. ZCPs-Q could significantly attenuate AAPH induced erythrocyte hemolysis through the inhibition of ROS generation...
November 8, 2018: Journal of Agricultural and Food Chemistry
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