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https://www.readbyqxmd.com/read/30552560/functional-teas-from-the-stems-of-penthorum-chinense-pursh-phenolic-constituents-antioxidant-and-hepatoprotective-activity
#1
Libo He, Shiyan Zhang, Chaomei Luo, Yiran Sun, Qiuxia Lu, Lei Huang, Fang Chen, Lin Tang
Penthorum chinense Pursh (PCP), a medicinal and edible plant, is traditionally used for liver protection and treatment of liver diseases. In this study, we compared the differences of composition and activity of flowers, stems and leaves of PCP to select a bioactive part. The stems of PCP with stronger antioxidant activity (6.25-100 μg/mL) and lower cytotoxicity (25-200 μg/mL) than the flowers and leaves were a better bioactive part. Then the chemical composition and hepatoprotective effects of an aqueous extract and an 70% ethanolic extract made with stems of PCP were investigated...
December 14, 2018: Plant Foods for Human Nutrition
https://www.readbyqxmd.com/read/30550712/unbiased-measurement-of-phosphate-and-phosphorus-speciation-in-surface-waters
#2
Vlastimil Packa, Stefanie Maedler, Todd Howell, Vadim Bostan, Ngan Diep, Robert J Tooley, Vasile I Furdui
Trace-level phosphate analysis and phosphorus speciation in surface water remained challenging due to adsorption and phosphate uptake by microorganisms. In this study a two-dimensional ion chromatography separation coupled to electrospray ionization high-resolution mass spectrometry (2D-IC-ESI-MS) allowed isotope dilution quantitation of phosphate with simultaneous analysis of eleven phosphate-containing metabolites and two inorganic condensed phosphates. Samples were collected from Lake St. Clair, Lake Ontario and Georgian Bay (ON, Canada)...
December 14, 2018: Environmental Science & Technology
https://www.readbyqxmd.com/read/30549457/investigations-into-the-performance-of-travelling-wave-enabled-conventional-and-cyclic-ion-mobility-systems-to-characterise-protomers-of-fluoroquinolone-antibiotic-residues
#3
Michael McCullagh, Kevin Giles, Keith Richardson, Sara Stead, Martin Palmer
Fluoroquinolones (FLQs) are a class of antimicrobial agents which have been administered to livestock for infection prevention/control and growth promotion. In this work blank porcine muscle extract (fortified with FLQs) has been screened for the FLQ protomer species (resulting from different sites of protonation) using the cumulative specificity of Ultra High Performance Liquid Chromatography coupled with Ion Mobility - Mass Spectrometry (UHPLC-IM-MS) and collision induced dissociation (CID). Collision cross sections (CCSs) and product ion spectra have been generated for the protomeric species with ΔCCS values between protomers ranging between 10-15 Å2 ...
December 14, 2018: Rapid Communications in Mass Spectrometry: RCM
https://www.readbyqxmd.com/read/30549079/quantification-and-pharmacokinetic-study-of-entrectinib-in-rat-plasma-using-ultra-performance-liquid-chromatography-tandem-mass-spectrometry
#4
Qiong Wang, Ping Fang, Lulu Zheng, Lei Ye
To characterize the preclinical plasma pharmacokinetics of entrectinib, a reproducible and precise assay is necessary. In this study, we developed and validated a simple ultra-performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS) method for the measurement of entrectinib using carbamazepine as the internal standard (IS) in rat plasma. Sample preparation was a simple protein precipitation with acetonitrile, then entrectinib was eluted on an Acquity UPLC BEH C18 column (2.1 mm × 50 mm, 1.7 μm) using a gradient elution with a mobile phase composed of acetonitrile (A) and 0...
December 13, 2018: Biomedical Chromatography: BMC
https://www.readbyqxmd.com/read/30548573/identification-of-new-urinary-gamma-hydroxybutyric-acid-ghb-markers-applying-untargeted-metabolomics-analysis-following-placebo-controlled-administration-to-humans
#5
Andrea E Steuer, Justine Raeber, Christian Steuer, Martina I Boxler, Dario A Dornbierer, Oliver G Bosch, Boris B Quednow, Erich Seifritz, Thomas Kraemer
Gamma-hydroxybutyrate (GHB) is a short-chain fatty acid that occurs naturally in the mammalian brain and that is prescribed as a medication against narcolepsy or used as a drug of abuse. Particularly, its use as a knock-out drug in cases of drug facilitated crimes is of major importance in forensic toxicology. Because of its rapid metabolism and resulting narrow detection windows (<12 h in urine), detection of GHB remains challenging. Thus, there is an urgent call for new markers to improve the reliable detection of GHB use...
December 12, 2018: Drug Testing and Analysis
https://www.readbyqxmd.com/read/30548501/a-quantitative-lc-ms-ms-method-for-determination-of-a-small-molecule-agonist-of-epha2-in-mouse-plasma-and-brain-tissue
#6
Bo Zhong, Yaxin Li, Nethrie Idippily, Aaron Petty, Bin Su, Bingcheng Wang
Compound 27 [1, 12-bis(4-(4-amino-6,7-dimethoxyquinazolin-2-yl)piperazin-1-yl)dodecane-1,12-dione ] is a novel small molecule agonist of EphA2 receptor Tyrosine Kinase. It showed much improved activity for the activation of EphA2 receptor compared to the parental compound Doxazosin. To support further pharmacological and toxicological studies of the compound, a method using liquid chromatography and electrospray ionization tandem mass spectrometry (LC- MS/MS) has been developed for the quantification of this compound...
December 12, 2018: Biomedical Chromatography: BMC
https://www.readbyqxmd.com/read/30548149/preparation-and-characterization-of-bosentan-monohydrate-%C3%AE%C2%B5-polycaprolactone-nanoparticles-obtained-by-electrospraying
#7
Virna M Giménez, Norma Sperandeo, Sonia Faudone, Sandra Noriega, Walter Manucha, Diego Kassuha
The electrospraying technique provides nano and microparticles that can be used as drug delivery systems. The aims of this study were, firstly, to optimize the influent parameters of electrospraying for the manufacture of a Bosentan (BOS) nanoparticulate platform, and secondly, to evaluate its physicochemical properties and in vitro biopharmaceutical behavior. Particles were characterized by scanning electron microscopy (SEM), powder X-ray diffraction (PXRD), differential scanning calorimetry (DSC), thermogravimetry (TG) and Fourier transformed Infrared spectroscopy (FTIR)...
December 8, 2018: Biotechnology Progress
https://www.readbyqxmd.com/read/30546988/time-resolved-universal-temperature-measurements-using-nayf-4-er-3-yb-3-upconverting-nanoparticles-in-an-electrospray-jet
#8
Kristina Shrestha, Arwa A Alaulamie, Ali Rafiei Miandashti, Hugh H Richardson
Hexagonal upconverting nanoparticles (UCNPs) of NaYF4 :Er3+ ,Yb3+ (ca. 300 nm) have been widely used to measure the temperature at the nanoscale using luminescence ratio thermometry. However, several factors limit their applications. For example, changes in the peak shape, mainly is the S-band emission, hinders their ability to be used as a universal temperature sensor. Herein, we introduce a universal calibration protocol for NaYF4 :Er3+ ,Yb3+ upconverting nanoparticles that is robust to environmental changes and gives a precise temperature measurement...
2018: Beilstein Journal of Nanotechnology
https://www.readbyqxmd.com/read/30545804/piper-auritum-kunth-piperaceae-improves-the-sexual-performance-of-sluggish-male-rats-through-enhancing-ejaculation
#9
Rosa Estrada-Reyes, Ana María Dorantes-Barrón, Daniel Arrieta-Báez, Mayra Beatriz Gómez-Patiño, Andrea Bernal-Trujillo, Mario Castro-García, Miguel Carro-Juárez, Lucía Martínez-Mota
ETHNOPHARMACOLOGICAL RELEVANCE: Piper auritum Kunth is employed as an aphrodisiac in the traditional medicine, but corroborative evidence for such effect is scarce. AIM OF THE STUDY: The pro-sexual effect of an aqueous extract of P. auritum and its possible mechanisms were analyzed in two paradigms of male sexual function. MATERIAL AND METHODS: Effects of an aqueous extract of P. auritum (PA, single administration) were investigated in the fictive ejaculation, and copulatory behavior paradigms in sexually sluggish male rats...
October 26, 2018: Journal of Ethnopharmacology
https://www.readbyqxmd.com/read/30545248/identification-of-lipids-of-the-stratum-corneum-by-high-performance-thin-layer-chromatography-and-mass-spectrometry
#10
Emilien L Jamin, Carine Jacques, Laëtitia Jourdes, Jean-Claude Tabet, Nathalie Borotra, Sandrine Bessou-Touya, Laurent Debrauwer, Hélène Duplan
The stratum corneum, the outermost layer of the epidermis, is the most important skin barrier against exogenous physical and chemical effects, in addition to protecting against dehydration. Ceramides are integral parts of the intercellular lipid lamellae of the stratum corneum and play an important role in the barrier function of mammalian skin. Ceramides are sphingolipids consisting of sphingoid bases linked to fatty acids by an amide bond. Typical sphingoid bases in the skin are composed of dihydrosphingosine, sphingosine, phytosphingosine, and 6-hydroxysphingosine, and the fatty acid acyl chains are composed of non-hydroxy fatty acid, α-hydroxy fatty acid, ω-hydroxy fatty acid, and esterified ω-hydroxy fatty acid...
December 13, 2018: European Journal of Mass Spectrometry
https://www.readbyqxmd.com/read/30544938/simultaneous-quantitation-of-isoprenoid-pyrophosphates-in-plasma-and-cancer-cells-using-lc-ms-ms
#11
Yashpal S Chhonker, Staci L Haney, Veenu Bala, Sarah A Holstein, Daryl J Murry
Isoprenoids (IsoP) are an important class of molecules involved in many different cellular processes including cholesterol synthesis. We have developed a sensitive and specific LC-MS/MS method for the quantitation of three key IsoPs in bio-matrices, geranyl pyrophosphate (GPP), farnesyl pyrophosphate (FPP), and geranylgeranyl pyrophosphate (GGPP). LC-MS/MS analysis was performed using a Nexera UPLC System connected to a LCMS-8060 (Shimadzu Scientific Instruments, Columbia, MD) with a dual ion source. The electrospray ionization source was operated in the negative MRM mode...
December 11, 2018: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/30544490/antioxidant-properties-and-the-formation-of-iron-coordination-complexes-of-8-hydroxyquinoline
#12
Vladimir Chobot, Franz Hadacek, Gert Bachmann, Wolfram Weckwerth, Lenka Kubicova
BACKGROUND: The alkaloid 8-hydroxyquinoline (8HQ) is well-known for various biological activities, including antioxidant effects and especially for the formation of coordination complexes with various transition metals, such as iron, amongst others. Therefore, 8HQ was extensively explored as a promising antineurodegenerative agent. However, other authors noted pro-oxidant effects of 8HQ. Here, we explore the pro- and antioxidant properties of 8HQ, especially in context of coordination complexes with iron (II) and iron (III)...
December 7, 2018: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/30543790/monitoring-novel-modified-hemoglobin-using-mass-spectrometry-contributes-to-accurate-blood-glucose-management-of-the-han-chinese-population
#13
Yunpeng Wu, Rui Zhou, Zhizhen Lai, Wei Wang, Na Li, Yuying Du, Lei Guo, Yuming Qiu, Qing-Tao Wang, Zhili Li
BACKGROUND: The goal of this study was to detect novel modified forms of hemoglobin using mass spectrometry (MS) and to investigate the effect of modified hemoglobin on HbA1c and fasting plasma glucose (FPG). METHODS: This study was conducted on 1200 subjects aged >25 years. Hemoglobin from the above-mentioned subjects was detected using direct-infusion electrospray ionization-MS, and HbA1c and FPG were measured according to the manufacturer's instructions...
December 10, 2018: Clinica Chimica Acta; International Journal of Clinical Chemistry
https://www.readbyqxmd.com/read/30543282/direct-single-and-double-side-triol-functionalization-of-the-mixed-type-anderson-polyoxotungstate-cr-oh-3-w-6-o-21-6
#14
Nadiia I Gumerova, Tania Caldera Fraile, Alexander Roller, Gerald Giester, Magda Pascual-Borràs, C André Ohlin, Annette Rompel
Since the first successful triol-functionalization of the Anderson polyoxometalates, the six protons of their central octahedron X(OH)6 (X-heteroatom, p- or d-element) have been considered as a prerequisite for their functionalization with tripodal alcohols, and therefore, the functionalization of Anderson structures from the unprotonated sides have never been reported. Here, we describe the triol-functionalization of [Cr(OH)3 W6 O21 ]6- leading to the single-side grafted anions [Cr(OCH2 )3 CRW6 O21 ]6- (CrW6 -tris-R, R = -C2 H5 , -NH2 , -CH2 OH) and the unprecedented double-side functionalized anion [Cr((OCH2 )3 CC2 H5 )2 W6 O18 ]3- (CrW6 -(tris-C2 H5 )2 ), despite the lack of protons in the parent anion in the solid state...
December 13, 2018: Inorganic Chemistry
https://www.readbyqxmd.com/read/30542678/dual-cationic-anionic-profiling-of-metabolites-in-a-single-identified-cell-in-a-live-xenopus-laevis-embryo-by-microprobe-ce-esi-ms
#15
Erika P Portero, Peter Nemes
In situ capillary microsampling with capillary electrophoresis (CE) electrospray ionization (ESI) mass spectrometry (MS) enabled the characterization of cationic metabolites in single cells in complex tissues and organisms. For deeper coverage of the metabolome and metabolic networks, analytical approaches are needed that provide complementary detection for anionic metabolites, ideally using the same instrumentation. Described here is one such approach that enables sequential cationic and anionic (dual) analysis of metabolites in the same identified cell in a live vertebrate embryo...
December 13, 2018: Analyst
https://www.readbyqxmd.com/read/30538462/dimethyl-2-2-2-2-ethane-1-1-diyl-bis-1-h-indole-3-2-diyl-diacetate-a-small-molecule-capable-of-nano-scale-assembly-inhibiting-venous-thrombosis-and-inducing-no-bleeding-side-effect
#16
Yaonan Wang, Haiyan Chen, Xiaoyi Zhang, Lin Gui, Jianhui Wu, Qiqi Feng, Shiqi Peng, Ming Zhao
Background: Due to the discovery that deep venous thrombosis (DVT) inhibitor is of chemotherapeutic importance, the nano-property of dimethyl 2,2'-[2,2'-(ethane-1,1-diyl) bis(1 H -indole-3,2-diyl)]-diacetate (DEBIC), a recently reported antitumor agent, is worthy of characterization. Materials and methods: One-pot reaction was used to prepare DEBIC. Electrospray Ionization (+/-)-Fourier Transform-Ion Cyclotron Resonance-Mass Spectrometer (ESI(+/-)-FT-ICR-MS), quadrupole Collision Induced Dissociation (qCID) and nuclear overhauser effect spectroscopy spectra were used to present the assembly of DEBIC...
2018: International Journal of Nanomedicine
https://www.readbyqxmd.com/read/30537624/an-lc-ms-ms-method-for-quantification-of-abiraterone-its-active-metabolites-d-4-abiraterone-d4a-and-5%C3%AE-abiraterone-and-their-inactive-glucuronide-derivatives
#17
Patrick Caron, Véronique Turcotte, Eric Lévesque, Chantal Guillemette
Abiraterone acetate (AA) is a prodrug of abiraterone, a selective and potent steroidal cytochrome P450 17alpha- hydroxylase-17,20-lyase (CYP17A1) blocking androgen synthesis in the treatment of advanced prostate cancer. Abiraterone (Abi) is metabolized to D(4)-abiraterone (D4A) directly blocking CYP17A1 and other steroidogenic enzymes and antagonizing the androgen receptor (AR). D4A is converted by 5α-reductase to 3-keto-5α-abiraterone (5α-Abi), an AR agonist. Our recent work suggests phase II biotransformation of Abi, D4A and 5α-Abi conjugated to glucuronic acid in vitro leading to four glucuronides (G)...
December 4, 2018: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/30536439/preparation-and-in-vitro-in-vivo-evaluation-of-anastrozole-reservoir-type-intravaginal-ring
#18
Liangyu Xia, Shunchen Qiu, Zhenqi Liu, Meiying Ning
This report details the preparation of anastrozole (ATZ) reservoir-type intravaginal ring (IVR) and the detection the concentration of ATZ in Beagle dog plasma by liquid chromatography-tandem mass spectrometry (LC-MS/MS). An ATZ reservoir-type IVR which included ATZ silicone elastomer core and non-active silicone layer, was manufactured by reaction injection moulding at 80°C for 20 min. In vitro release experiment was performed under sink conditions and the samples are determined by high performance liquid chromatography (HPLC)...
December 9, 2018: Biomedical Chromatography: BMC
https://www.readbyqxmd.com/read/30535889/oligomeric-transition-and-dynamics-of-rna-binding-by-the-hur-rrm1-domain-in-solution
#19
Carolina Lixa, Amanda Mujo, Mariana T Q de Magalhães, Fabio C L Almeida, Luis Mauricio T R Lima, Anderson S Pinheiro
Human antigen R (HuR) functions as a major post-transcriptional regulator of gene expression through its RNA-binding activity. HuR is composed by three RNA recognition motifs, namely RRM1, RRM2, and RRM3. The two N-terminal RRM domains are disposed in tandem and contribute mostly to HuR interaction with adenine and uracil-rich elements (ARE) in mRNA. Here, we used a combination of NMR and electrospray ionization-ion mobility spectrometry-mass spectrometry (ESI-IMS-MS) to characterize the structure, dynamics, RNA recognition, and dimerization of HuR RRM1...
December 8, 2018: Journal of Biomolecular NMR
https://www.readbyqxmd.com/read/30535238/association-between-sphingolipids-and-cardiopulmonary-fitness-in-coronary-artery-disease-patients-undertaking-cardiac-rehabilitation
#20
Mahwesh Saleem, Nathan Herrmann, Adam Dinoff, Susan Marzolini, Michelle M Mielke, Ana Andreazza, Paul I Oh, Swarajya Lakshmi Vattem Venkata, Norman J Haughey, Krista L Lanctôt
The long term benefits conferred by cardiac rehabilitation (CR) in those with CAD are strongly linked with an improvement in cardiopulmonary fitness. This study aimed to determine the association between peripheral sphingolipids and cardiopulmonary fitness in CAD subjects undertaking CR. Patients with CAD (n=100, mean age=64±6 years, 85% male, mean years of education=17±3 years) underwent 6 months of CR with blood collected at baseline, 3 and 6 months. Cardiopulmonary fitness was assessed by measuring peak oxygen uptake (VO2peak) at all time points...
December 8, 2018: Journals of Gerontology. Series A, Biological Sciences and Medical Sciences
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