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S Feuerbaum, N Saile, G Pohlentz, J Müthing, H Schmidt
Enterohemorrhagic Escherichia coli (EHEC) O157:H7 strain EDL933 encodes the single chromosomal 9-O-acetylesterase NanS, and several copies of prophage-encoded 9-O-acetylesterases (NanS-p). These enzymes have recently been shown to cleave 5-N-acetyl-9-O-acetyl neuraminic acid (Neu5,9Ac2 ) to yield de-O-acetylated Neu5Ac, the latter of which may serve as a carbon and/or nitrogen source. In the current study, we investigated the NanS- and NanS-p-mediated digestion of synthetic O-acetylated neuraminic acids and bovine submaxillary glands mucin (BSM)-derived O-acetylneuraminic acids by high-performance thin-layer chromatography (HPTLC) and nano electrospray ionization mass spectrometry (nanoESI MS)...
October 9, 2018: International Journal of Medical Microbiology: IJMM
Baoxin Xu, Congcong Qu, Wenhua Zheng, Yaya Xi, Xin Zhao, Huifen Li, Jiangting Liu, Xuelan Zhang
Radix Polygala (Yuanzhi in Chinese) is well-known in traditional Chinese medicine (TCM) and has been used for treatment of depression, brain protection, and memory improvement for thousands of years. This plant medicine is rich in saponins, glycolipids, and organic acids. The purpose of the current study was to develop a rapid, accurate, and sensitive ultra-high performance liquid chromatography tandem mass spectrometry (UHPLC-MS/MS) method for the simultaneous determination of the following seven active components of Radix Polygala extracts in rat plasma: sibiricose A5 (A5); sibiricose A6 (A6); 3,6'-disinapoyl sucrose (DSS); tenuifoliside A (TFSA); tenuifoliside B (TFSB); tenuifoliside C (TFSC); and 3,4,5-trimethoxycinnamic acid (TMCA)...
October 2, 2018: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
Xiunan Jiang, Yiting Shen, Haixing Wang, Caihong Wang, Xiaoxia Ye, Zheng Xiang
Kaurenoic acid (KA), a kaurane diterpene found in several medicinal plants, is an active ingredient with potential anti-inflammatory, anticonvulsant, antibacterial and antitumor activities. In this work, an ultra-performance liquid chromatography-tandem mass spectrometry method (UPLC-MS/MS) was firstly developed and validated to quantify kaurenoic acid in rat plasma. Rhein was chosen as the internal standard (IS) and the plasma was processed with one-step acetonitrile protein precipitation; the chromatographic separation was achieved on a HSS T3 (2...
October 9, 2018: Journal of Pharmaceutical and Biomedical Analysis
Jiangfeng Ye, Wenting Zhu, Han Liu, Yuchan Mao, Fan Jin, Jun Zhang
OBJECTIVES: Polycystic ovary syndrome (PCOS) is the most common endocrinopathy of women at reproductive age. Although the aetiology of PCOS remains unclear, potential effects of environmental endocrine-disrupting compounds on the development of PCOS have drawn increasing attention. The aim of the current study was to examine the association between triclosan (TCS) and PCOS, and explore possible mechanisms on how TCS may contribute to the development of clinical manifestations of PCOS...
October 17, 2018: BMJ Open
Praveen Satapute, Milan V Kamble, Shivakantkumar S Adhikari, Sudisha Jogaiah
Pesticides are generally applied to agricultural soil to control crop diseases, with the critical goal of preventing yield loss and optimizing economic returns. However, the excessive utilization of pesticides in agriculture is a severe environmental and human health problem. In the present study, effect of triazole based fungicide, propiconazole on soil physicochemical properties, stimulatory impact of propiconazole residue on soil microbial communities and soil enzyme activities (phosphatise and urease) in contaminated red sandy loam and deep black soils of paddy (Oryza sativa L...
October 9, 2018: Science of the Total Environment
Chun-Xiang Geng, Na Cao, Wei Xu, Chen He, Zi-Wen Yuan, Jin-Wei Liu, Quan Shi, Chun-Ming Xu, Si-Tong Liu, Hua-Zhang Zhao
Coagulation is an important process to remove organics from water. The molecular composition and structure of organic matter influence water quality in many ways, and the lack of information regarding the organics removed by different coagulants makes it challenging to optimize coagulation processes and ensure reclaimed water safety. In this paper, we investigated coagulation of secondary biological effluent from a municipal sewage treatment plant with different coagulants. We emphasized investigation of organics removal characteristics at the molecular level using Fourier transform-ion cyclotron resonance mass spectrometry (FT-ICR MS) coupled with electrospray ionization (ESI)...
October 18, 2018: Environmental Science & Technology
Lei Yin, Zhi Zhang, Yingze Liu, Yin Gao, Jingkai Gu
Cells are the most basic structural units that play vital roles in the functioning of living organisms. Analysis of the chemical composition and content of a single cell plays a vital role in ensuring precise investigations of cellular metabolism, and is a crucial aspect of lipidomic and proteomic studies. In addition, structural knowledge provides a better understanding of cell behavior as well as the cellular and subcellular mechanisms. However, single-cell analysis can be very challenging due to the very small size of each cell as well as the large variety and extremely low concentrations of substances found in individual cells...
October 18, 2018: Analyst
Tomasz Tuzimski, Dominika Pieniążek, Grzegorz Buszewicz, Grzegorz Teresiński
Different extraction and clean-up protocols, based on either the dispersive solid-phase extraction (d-SPE) and the quick, easy, cheap, effective, rugged, and safe (QuEChERS) approach, were optimized and compared for the determination of selected bisphenols such as bisphenol A (BPA) and bisphenol S (BPS) in breast milk samples. QuEChERS-based sample preparation procedure was optimized by evaluation of different clean-up sorbents including zirconium-based sorbents (Z-Sep and Z-Sep Plus) and primary secondary amine...
October 17, 2018: Journal of AOAC International
Zhenqing Li, Ziyan Zhao, Zhiqin Zhou
In this study, a preparative separation method was established to simultaneously isolate the polymethoxylated flavones (PMFs) from the peel of "Dahongpao" tangerine using macroporous adsorptive resins (MARs) combined with prep-HPLC. The total PMFs were enriched using MARs to remove most sugars, water-soluble pigments, and flavanones, and the eluents obtained were analyzed by ultra-performance liquid chromatography (UPLC) to determine the PMF composition. The separation and purification of PMFs were carried out by using a mass spectrometry-guided prep-HPLC with a gradient elution of acetonitrile-water ( v / v ), simultaneously...
October 16, 2018: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
Kenji Honma
The gas-phase fluorescence excitation and emission properties of rhodamine 590 (R590) and rhodamine 640 (R640) cations were studied using a novel instrument that allows extension of optical excitation to the UV region. Ions were generated by an electrospray ion source, and desolvated ions were accumulated in a room-temperature quadrupole ion-trap. In addition to the well-known S1-S0 transition observed for both dyes, two ultraviolet (UV) bands (that have not been reported previously) were observed for R590...
October 17, 2018: Physical Chemistry Chemical Physics: PCCP
E Humer, I Kröger, V Neubauer, N Reisinger, Q Zebeli
Grain-rich diets often lead to subacute ruminal acidosis (SARA) impairing rumen and systemic cattle health. Recent data suggest beneficial effects of a clay mineral (CM)- based product on the rumen microbiome of cattle during SARA. This study sought to investigate whether the CM supplementation can counteract SARA-induced perturbations of the bovine systemic health. The study used an intermittent diet-induced SARA-model with eight dry Holstein cows receiving either no additive as control or CM via concentrates (n=8 per treatment)...
October 17, 2018: Animal: An International Journal of Animal Bioscience
Jacob P Walsh, Justin B Renaud, Shawn Hoogstra, David R McMullin, Ashraf Ibrahim, Cobus Visagie, Joey B Tanney, Ken K-C Yeung, Mark W Sumarah
RATIONALE: Microbial natural products are often biosynthesized as classes of structurally related compounds that have similar MS/MS fragmentation patterns. Mining MS/MS datasets for precursor ions that share diagnostic or common features enables entire chemical classes to be identified, including novel derivatives that have previously been unreported. Analytical data analysis tools that can facilitate a class-targeted approach to rapidly dereplicate known compounds and identify structural variants within complex matrices would be useful for the discovery of new natural products...
October 16, 2018: Rapid Communications in Mass Spectrometry: RCM
Yashpal S Chhonker, Liping Ma, Constant Edi, Daryl J Murry
AIM: A sensitive and selective LC-MS/MS method was validated for quantitation of ivermectin (IVM) in plasma. METHOD: The IVM was extracted from plasma using solid-phase extraction with C-18 cartridges. Separation of analytes was achieved on an ACE C18 column with isocratic elution using 0.1% acetic acid and methanol: acetonitrile (1:1, v/v) as mobile phase. The IVM was quantitated using electrospray ionization operating in negative multiple reaction monitoring mode...
October 16, 2018: Bioanalysis
Zhijun Wang, Chen Xie, Steven Yeung, Jeffrey Wang, Moses S Chow
Streptomycin is the first discovered aminoglycoside antibiotic. Streptomycin has been widely applied in veterinary medicine for prevention and treatment of bacterial infection. However, the current detection methods are not satisfactory in terms of sensitivity and sample process which made them not suitable for a pharmacokinetic study. A high-performance liquid chromatography-mass spectrometric (HPLC-MS/MS) method employing positive electrospray ionization was developed and validated for the determination of streptomycin concentration in mice plasma...
October 15, 2018: Biomedical Chromatography: BMC
María de la Luz Cádiz-Gurrea, Ignacio Fernández de Las Nieves, Luis Manuel Aguilera Saez, Salvador Fernández-Arroyo, Laurence Legeai-Mallet, Mohamed Bouaziz, Antonio Segura-Carretero
Plants, including most food and feed plants, produce a broad range of bioactive chemical compounds. Among these compounds, polyphenols are reported to provide beneficial effects as anti-carcinogenic, anti-atherogenic, anti-inflammatory, immune modulating, anti-microbial, vasodilatory and analgesic. Cocoa (Theobroma cacao), a major, economically important, international crop, has been related to several nutritional benefits, which have been associated with the phenolic fraction. The main subclass of flavonoids found in cocoa is flavanols, particularly (epi)catechins monomers, and their oligomers, also known as procyanidins...
October 15, 2018: Plant Foods for Human Nutrition
Hung Su, Min-Zong Huang, Jo-Han Chou, Ting-Hao Chang, Yu-Min Jiang, Yi-Tzu Cho, Sy-Chyi Cheng, Ming-Tsang Wu, Jentaie Shiea
High-throughput screening of plastic products in children's living environment is necessary to identify phthalate-containing objects for the concern of public health and safety. A novel strategy of probe collecting technique combined with ambient mass spectrometry was developed to carry out the large-scale sample analysis. Analytes from the surface of approximately 500 objects each in two kindergartens in Taiwan were collected using the same number of the metallic probes. After being delivered to laboratory, the analytes on the probes were analyzed with thermal desorption-electrospray ionization/mass spectrometry (TD-ESI/MS)...
December 18, 2018: Analytica Chimica Acta
Sonia Emanuele, Antonietta Notaro, Antonio Palumbo Piccionello, Antonella Maggio, Marianna Lauricella, Antonella D'Anneo, Cesare Cernigliaro, Giuseppe Calvaruso, Michela Giuliano
Litchi chinensis Sonnerat is a tropical tree whose fruits contain significant amounts of bioactive polyphenols. Litchi cultivation has recently spread in Sicily where the climate conditions are particularly favorable for this crop. Recent findings have shown that Litchi extracts display anti-tumor and pro-apoptotic effects in vitro, but the precise underlying mechanisms have not been fully elucidated. In this study, we report for the first time the effects of Sicilian litchi fruit extracts on colon cancer cells...
October 12, 2018: Nutrients
Jinli Qiu, Fuqiang Liu, Cailiang Yue, Chen Ling, Aimin Li
A novel nanosheet of Mo/N-codoped TiO2 nanorods immobilized on carbon nanofibers (MNTC nanosheet) was self-synthesized through two facile steps. The Mo/N-doped TiO2 nanorods dispersed through in situ growth on the network constructed by long and vertical carbon nanofibers (CNFs). The fabricated MNTC nanosheet displayed superb photocatalytic activity of methylene blue (MB), and the degradation ratio by the MNTC nanosheet was nearly twice than that of pure nanoparticles. The photocatalytic activities during the degradation process in the presence of environmental media such as inorganic salts and natural organic matter (NOM) were also determined...
October 8, 2018: Chemosphere
Arghyadeep Bhattacharyya, Subhash Chandra Makhal, Nikhil Guchhait
We report the synthesis of a novel hydrazine-bridged anthranilic acid-naphthalene conjugate ( E )-2-(benzamido)- N '-((2-hydroxynaphthalen-1-yl) methylene) benzohydrazide ( BBHAN ) and its crystal structure. BBHAN can detect Al3+ by a sharp increment in fluorescence intensity (∼40 times) in aqueous methanolic medium. The limit of detection of BBHAN towards Al3+ is 1.68 × 10-9 M, and the former undergoes a 2:1 binding with Al3+ with a high binding constant of ∼1.15 × 1011 M2- . BBHAN detects Al3+ by the well-known mechanism of chelation-enhanced fluorescence (CHEF), established by fluorescence time-resolved measurement...
September 30, 2018: ACS Omega
Yuezhou Liu, Bingbing Shi, Hu Wang, Liqing Shangguan, Zhengtao Li, Mingming Zhang, Feihe Huang
In this work, a Pd2 L4 metallacage 2•([BF4 ]- )4 with four pillar[5]arene units is first prepared and characterized by 1D multinuclear NMR (1 H, 11 B, and 19 F NMR), 2D 1 H-1 H correlation spectra, 1 H-13 C heteronuclear single quantum coherence, and diffusion-ordered NMR spectroscopy, and electrospray ionization time-of-flight mass spectrometry. By the introduction of a ditopic guest molecule 3 into a chloroform solution of 2•([BF4 ]- )4 , a supramolecular polymer network gel is successfully constructed based on the metal coordination interactions and host-guest recognition between the pillar[5]arene units of 2•([BF4 ]- )4 and neutral ditopic guest molecule 3...
October 15, 2018: Macromolecular Rapid Communications
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