Read by QxMD icon Read

Highly sensitized kidney recipients

Nele Kirsten Kanzelmeyer, Petra Zürbig, Harald Mischak, Jochen Metzger, Alexander Fichtner, Kristzina Heindl Ruszai, Tomas Seemann, Matthias Hansen, Simone Wygoda, Kai Krupka, Burkhard Tönshoff, Anette Melk, Lars Pape
Chronic antibody-mediated rejection (cABMR) is the main cause of long-term renal graft loss. Late-stage diagnosis is made by detecting donor-specific antibodies (DSA) in blood combined with typical histomorphological lesions in renal allografts. There is a need for non-invasive biomarkers for cABMR that might permit screening and earlier diagnosis. In a case control study of 24 pediatric renal transplant recipients urine samples were analyzed using capillary electrophoresis and mass spectrometry. Patients were matched with 36 pediatric renal transplant patients without cABMR...
October 25, 2018: Transplant International: Official Journal of the European Society for Organ Transplantation
Aureliusz Kolonko, Beata Bzoma, Piotr Giza, Beata Styrc, Michał Sobolewski, Jerzy Chudek, Alicja Dębska-Ślizień, Andrzej Więcek
Background : The panel-reactive antibodies that use the complement-dependent cytotoxicity test (PRA-CDC) are still a standard method for monitoring the degree of immunization in kidney transplant candidates on active waiting lists in some countries, including Poland. The aim of this study was to analyze the relationship between the maximum and the last pre-transplant PRA titer on the percentage of positive cross-matches and rate of early acute rejection episodes. Material and methods : The retrospective analysis included 528 patients from two transplant centers...
September 20, 2018: Medicina
Kristen Sgambat, Sarah Clauss, Asha Moudgil
BACKGROUND: Kidney transplant recipients are at high risk for CV morbidity. However, the measure of obesity that best predicts CV risk has not been established. OBJECTIVE: A prospective, controlled study was conducted to compare the ability of BMI, WC, and WHr to identify CV risk in pediatric kidney transplant recipients. METHODS: Transplant recipients, aged 3-20 years, had echocardiogram, CIMT, BMI, WC, WHr, blood pressure, lipids, and leptin measured...
December 2018: Pediatric Transplantation
Elisabet Estil Les, Noèlia Téllez, Montserrat Nacher, Eduard Montanya
Streptozotocin (STZ) is a cytotoxic glucose analogue that causes beta cell death and is widely used to induce experimental diabetes in rodents. The sensitivity of beta cells to STZ is species-specific and human beta cells are resistant to STZ. In experimental islet transplantation to rodents, STZ-diabetes must be induced before transplantation to avoid destruction of grafted islets by STZ. In human islet transplantation, injection of STZ before transplantation is inconvenient and costly, since human islet availability depends on organ donation and frail STZ-diabetic mice must be kept for unpredictable lapses of time until a human islet preparation is available...
October 1, 2018: Cell Transplantation
Renata von Glehn Ponsirenas, Helena B Cazarote, Stanley de Almeida Araújo, David Campos Wanderley, Silvia Shimakura, Joana S Valdameri, Fabiana L C Contieri, Cristina C Q C von Glehn, Michelle F Susin, Vanessa Santos Sotomaior
Background: The identification of low-level antibodies by single-antigen bead methodology has brought advancements to risk evaluation of kidney transplant recipients. However, the use of mean fluorescence intensity (MFI) to quantify antibodies and to guide therapy is not enough. Notably, immunoglobulin G (IgG) subclass switching is hypothesized to follow a programmed sequence after an emergency signal from the germinal center. In transplantation this process is not clear yet. In the present study, we sequentially evaluate anti-HLA donor specific antibody (DSA) subclasses, their profile changes, and C1q-binding ability and the influence of those characteristics on antibody mediated rejection (AMR) occurrence and allograft function...
September 2018: Transplantation Direct
David Solar-Cafaggi, Lluvia Marino, Norma Uribe-Uribe, Luis Eduardo Morales-Buenrostro
BACKGROUND: Antibody-mediated rejection (ABMR) is the leading cause of kidney graft loss worldwide. Criteria for acute humoral rejection (currently labeled active humoral rejection) established by the 2007 Banff classification are highly specific but lack sensitivity. Modifications to the Banff classification were introduced for its 2013 and 2017 versions in order to identify more cases of this entity. PURPOSE: We intend to demonstrate that, compared to its 2007 version, the 2017 Banff classification bears an improved capacity for graft loss prediction when histologic criteria for active ABMR are met...
August 29, 2018: Transplant Immunology
E Brochot, V Descamps, L Handala, J Faucher, G Choukroun, F Helle, S Castelain, C François, G Duverlie, A Touzé
OBJECTIVES: After kidney transplantation, human BK polyomavirus (BKPyV) can induce a progressive disease, in three stages: viruria, viraemia, and then nephropathy after a few months of viral replication. Therapeutic intervention is recommended when BKPyV is detected in the plasma. The objective of our study was to assess urinary BKPyV nucleic acid test as a predictor for developing viraemia. METHODS: We first defined a viruria threshold based on 393 time-matched urine and plasma samples collected after kidney transplantation; to validate this threshold, we followed-up a cohort of 236 kidney transplant patients...
August 1, 2018: Clinical Microbiology and Infection
D Kamińska, M Łukaszewski, N Kozera, K Kościelska-Kasprzak, D Bartoszek, O Mazanowska, W Goździk, P Chudoba, P Zaleska, A Lepiesza, M Klinger
Previously transplanted highly sensitized patients experience problems with subsequent transplantation. It is also difficult to provide optimal hemodynamic conditions during successive kidney transplantation in heart transplant recipients. PATIENT AND METHODS: We present a case of a 56-year old patient with end-stage renal failure after heart transplantation performed 21 years ago and hemodialyzed using arteriovenous fistula. The patient had 69% panel-reactive antibodies, had been on the active waiting list since 2013, and presented 335 positive crossmatches with deceased donors...
July 2018: Transplantation Proceedings
J Malyszko, E Koc-Żórawska, J S Malyszko
INTRODUCTION: Endocan is a novel soluble dermatan sulfate proteoglycan derived from endothelium. It has the capacity of binding to different biologically active molecules associated with cellular signaling, adhesion and regulating proliferation, differentiation, migration, and adhesion of different cell types in health and pathology. Elevated endocan levels are connected with endothelial activation/damage, neo-angiogenesis, and inflammation or carcinogenesis. MATERIALS AND METHODS: The level of serum endocan among 63 kidney transplant recipients on three immunosuppressives (calcineurin inhibitors, mycophenolate mofetil, steroids) in correlation with other markers of endothelial damage was estimated...
July 2018: Transplantation Proceedings
Elisa Gabanti, Daniele Lilleri, Lucia Scaramuzzi, Paola Zelini, Teresa Rampino, Giuseppe Gerna
Human cytomegalovirus (HCMV)-specific T-cell response in kidney transplant recipients (KTR) helps to identify patients at risk for severe infection. To assess the T-cell response, this study compared our in-house developed reference test, based on T-cell (both CD4+ and CD8+) stimulation by autologous HCMV-infected dendritic cells (iDC) and subsequent detection by cytokine flow cytometry (CFC-iDC), with the Quanti-FERON-CMV (QF-CMV) assay. Fifty-three HCMV-seropositive KTR were enrolled. At the DNAemia peak, 33 (62%) had low viral load (LVL, <3x105 DNA copies/mL) self-resolving infection, 19 (36%) high viral load (HVL, >3x105 DNA copies/mL) infection treated with antivirals, and one LVL patient (2%) tissue-invasive disease alone...
July 2018: New Microbiologica
Daqiang Zhao, Tao Liao, Siwen Li, Yannan Zhang, Haofeng Zheng, Jing Zhou, Fei Han, Yu Dong, Qiquan Sun
Antibody-mediated rejection (AMR) is the main barrier to renal graft survival, and mouse renal AMR models are important to study this process. Current mouse models are established by priming the recipient to donor skin for over 7 days before kidney transplantation. The robustness of AMR in these cases is too strong to mimic clinical AMR and it is unclear why altering the priming times ranging from 7 to 91 days fails to reduce the AMR potency in these models. In the present study, we found that the donor-recipient combination and skin graft size were determinants of donor-specific antibody (DSA) development patterns after skin transplantation...
2018: Frontiers in Immunology
A Kowada
OBJECTIVES: Tuberculosis (TB) is a serious infectious disease with high mortality for solid-organ transplantation. Preventive therapy of latent tuberculosis infection (LTBI) has been considered to reduce TB risk and improve outcomes of transplantation. The aim of this study was to evaluate the cost-effectiveness of the interferon-gamma release assays (IGRAs); QuantiFERON® -TB Gold in-Tube (QFT) and T-SPOT® .TB (TSPOT)), for kidney, liver and lung transplant recipients in low TB incidence countries...
July 24, 2018: Journal of Infection
Andreas M Farkas, Ulrike Baranyi, Georg A Böhmig, Lukas Unger, Stefan Hopf, Markus Wahrmann, Heinz Regele, Benedikt Mahr, Christoph Schwarz, Karin Hock, Nina Pilat, Ivan Kristo, Jasmin Mraz, Christian Lupinek, Josef Thalhamer, Gregor Bond, Lorenz Kuessel, Elizabeth Wlodek, Jack Martin, Menna Clatworthy, Gavin Pettigrew, Rudolf Valenta, Thomas Wekerle
BACKGROUND: Donor-specific antibodies of the IgG isotype are measured routinely for diagnostic purposes in renal transplant recipients and are associated with antibody-mediated rejection and long-term graft loss. OBJECTIVE: This study aimed to investigate whether MHC-specific antibodies of the IgE isotype are induced during allograft rejection. METHODS: Anti-MHC/HLA IgE levels were measured in sera of mice grafted with skin or heart transplants from various donor strains and in sera of kidney transplant patients with high levels of HLA IgG...
August 9, 2018: Journal of Allergy and Clinical Immunology
Andrew Bostom, Andreas Pasch, Tracy Madsen, Mary B Roberts, Nora Franceschini, Dominik Steubl, Pranav S Garimella, Joachim H Ix, Katherine R Tuttle, Anastasia Ivanova, Theresa Shireman, Reginald Gohh, Basma Merhi, Petr Jarolim, John W Kusek, Marc A Pfeffer, Simin Liu, Charles B Eaton
BACKGROUND: "T50," shortened transformation time from primary to secondary calciprotein particles may reflect deranged mineral metabolism predisposing to vascular calcification and cardiovascular disease (CVD). The glycoprotein fetuin-A is a major T50 determinant. METHODS: The Folic Acid For Vascular Outcome Prevention In Transplantation (FAVORIT) cohort is a completed, large, multiethnic controlled clinical trial cohort of chronic, stable kidney transplant recipients (KTRs)...
2018: American Journal of Nephrology
Maria Meneghini, Edoardo Melilli, Jaume Martorell, Ignacio Revuelta, Elisabet Rigol-Monzó, Anna Manonelles, Nuria Montero, David Cucchiari, Fritz Diekmann, Josep M Cruzado, Salvador Gil-Vernet, Josep M Grinyó, Oriol Bestard
Introduction: Despite the different assays available for immune-risk stratification before living-donor kidney transplantation (LDKT), the precise type and number of tests to perform remain uncertain. Methods: In a cohort of 330 consecutive LDKT patients, all of which were complement-dependent cytotoxicity (CDC)-crossmatch negative, we retrospectively analyzed the impact on main clinical outcomes of most sensitive immunoassays (complement-dependent cytotoxicity-panel-reactive antibody [CDC-PRA], flow cytometry crossmatch [FC-XM], donor-specific antibodies [DSAs], and their complement-binding capacity DSA-C3d]), together with donor/recipient HLA eplet matching...
July 2018: KI Reports
Marcos Vinicius de Sousa, Ana Claudia Gonçalez, Ricardo de Lima Zollner, Marilda Mazzali
BACKGROUND Donor-specific antibodies (DSA), directed against human leucocyte antigens (HLA), are associated with increased risk for graft rejection in kidney transplantation. Anti-HLA antibodies detection by Luminex™ present high sensitivity and accuracy, but its interpretation after transplantation is not completely clear. The aim of this study was to evaluate the impact of anti-HLA antibodies, preformed or de novo, on renal function, graft survival, and incidence of antibody-mediated acute rejection (AMR)...
July 6, 2018: Annals of Transplantation: Quarterly of the Polish Transplantation Society
Dorian Kulifaj, Bénédicte Durgueil-Lariviere, Faustine Meynier, Eliza Munteanu, Nicolas Pichon, Manon Dubé, Martine Joannes, Marie Essig, Sébastien Hantz, Côme Barranger, Sophie Alain
BACKGROUND: Torque teno viruses (TTV) are small DNA viruses whose replication is closely linked to immune status. A growing number of publications underlined the potential of TTV viral load as an indicator of immunosuppression. OBJECTIVES: To demonstrate the analytical performance of the first standardized RUO (Research Use Only) assay to detect and quantify human TTV DNA in whole blood and plasma. STUDY DESIGN: We established analytical performances for TTV load measurement in various populations...
August 2018: Journal of Clinical Virology: the Official Publication of the Pan American Society for Clinical Virology
Miriam Manook, Jean Kwun, Steven Sacks, Anthony Dorling, Nizam Mamode, Stuart Knechtle
Thrombotic microangiopathy (TMA) is a histological feature of antibody-mediated rejection and has the potential to cause problematic graft dysfunction, particularly for highly sensitized cross-match positive kidney transplant recipients. Prompt recognition of pertinent histopathological and systemic features of TMA in kidney transplantation is necessary. Underlying mechanisms of this process involve the activation of both complement and coagulation systems as a response to HLA antibody. As serine proteases, coagulation and complement cascades exhibit similar characteristics with respect to homeostatic function...
July 2018: Transplantation Reviews
Mehri Barabadi, Sanaz Keshavarz Shahbaz, Farshad Foroughi, Morteza Hosseinzadeh, Mohsen Nafar, Mir Saeed Yekaninejad, Aliakbar Amirzargar
BACKGROUND: Diagnosis of allograft dysfunction by noninvasive biomarker tests is preferable to invasive allograft biopsies and has been extensively considered in recent years. This study aims to evaluate blood and urinary forkhead box P3 (FOXP3) messenger RNA (mRNA) expression in renal transplant recipients in an attempt to determine whether differential diagnosis of graft dysfunction is feasible using mRNA profiles. METHODS: We analyzed FOXP3 mRNA expression in paired urinary and peripheral blood mononuclear cell (PBMC) samples...
June 2018: Progress in Transplantation
Matthew Kadatz, Scott Klarenbach, Jagbir Gill, John S Gill
Kidneys from deceased donors who are hepatitis C virus (HCV) nucleic acid test positive are infrequently used for transplantation in HCV-negative patients due to concerns about disease transmission. With the development of direct-acting antivirals (DAAs) for HCV, there is now potential to use these kidneys in HCV-negative candidates. However, the high cost of DAAs poses a challenge to adoption of this strategy. We created a Markov model to examine the cost-effectiveness of using deceased donors infected with HCV for kidney transplantation in uninfected waitlist candidates...
October 2018: American Journal of Transplantation
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"