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https://www.readbyqxmd.com/read/28528701/expansion-and-purification-are-critical-for-the-therapeutic-application-of-pluripotent-stem-cell-derived-myogenic-progenitors
#1
Jaemin Kim, Alessandro Magli, Sunny S K Chan, Vanessa K P Oliveira, Jianbo Wu, Radbod Darabi, Michael Kyba, Rita C R Perlingeiro
Recent reports have documented the differentiation of human pluripotent stem cells toward the skeletal myogenic lineage using transgene- and cell purification-free approaches. Although these protocols generate myocytes, they have not demonstrated scalability, safety, and in vivo engraftment, which are key aspects for their future clinical application. Here we recapitulate one prominent protocol, and show that it gives rise to a heterogeneous cell population containing myocytes and other cell types. Upon transplantation, the majority of human donor cells could not contribute to myofiber formation...
May 18, 2017: Stem Cell Reports
https://www.readbyqxmd.com/read/28512922/effect-of-chemical-immobilization-of-sdf-1%C3%AE-into-muscle-derived-scaffolds-on-angiogenesis-and-muscle-progenitor-recruitment
#2
Sareh Rajabi-Zeleti, Sasan Jalili-Firoozinezhad, Mohammad Kazemi Ashtiani, Gilles Le Carrou, Shahragim Tajbakhsh, Hossein Baharvand
The availability of three-dimensional bioactive scaffolds with enhanced angiogenic capacity that have the capability to recruit tissue specific resident progenitors is of great importance for the regeneration of impaired skeletal muscle. Here we have investigated whether introduction of chemoattractant factors to tissue specific extracellular matrix (ECM) promotes cellular behavior in vitro as well as muscle progenitor recruitment and vascularization in vivo. We developed an interconnective macroporous sponge from decellularized skeletal muscle with maintained biochemical traits of the intact muscle...
May 16, 2017: Journal of Tissue Engineering and Regenerative Medicine
https://www.readbyqxmd.com/read/28509082/atg7-mediated-autophagy-is-involved-in-the-neural-crest-cell-generation-in-chick-embryo
#3
Guang Wang, En-Ni Chen, Chang Liang, Jianxin Liang, Lin-Rui Gao, Manli Chuai, Andrea Münsterberg, Yongping Bao, Liu Cao, Xuesong Yang
Autophagy plays a very important role in numerous physiological and pathological events. However, it still remains unclear whether Atg7-induced autophagy is involved in the regulation of neural crest cell production. In this study, we found the co-location of Atg7 and Pax7(+) neural crest cells in early chick embryo development. Upregulation of Atg7 with unilateral transfection of full-length Atg7 increased Pax7(+) and HNK-1(+) cephalic and trunk neural crest cell numbers compared to either Control-GFP transfection or opposite neural tubes, suggesting that Atg7 over-expression in neural tubes could enhance the production of neural crest cells...
May 16, 2017: Molecular Neurobiology
https://www.readbyqxmd.com/read/28506310/unexpected-evolutionarily-conserved-rapid-effects-of-viral-infection-on-oxytocin-receptor-and-tgf-%C3%AE-psmad3
#4
Yutong Liu, Irina Conboy
BACKGROUND: shRNA lentiviral vectors are extensively used for gene knockdowns in mammalian cells, and non-target shRNAs typically are considered the proper experimental control for general changes caused by RNAi. However, the effects of non-target lentivirus controls on the modulation of cell signaling pathways remain largely unknown. In this study, we evaluated the effect of control lentiviral transduction on oxytocin receptor (OXTR) expression through the ERK/MAPK pathway in mouse and human skeletal muscle cells, on myogenic activity, and in vivo on mouse muscle regeneration...
May 15, 2017: Skeletal Muscle
https://www.readbyqxmd.com/read/28498863/notch-ligands-regulate-the-muscle-stem-like-state-ex-vivo-but-are-not-sufficient-for-retaining-regenerative-capacity
#5
Hiroshi Sakai, Sumiaki Fukuda, Miki Nakamura, Akiyoshi Uezumi, Yu-Taro Noguchi, Takahiko Sato, Mitsuhiro Morita, Harumoto Yamada, Kunihiro Tsuchida, Shahragim Tajbakhsh, So-Ichiro Fukada
Myogenic stem cells are a promising avenue for the treatment of muscular disorders. Freshly isolated muscle stem cells have a remarkable engraftment ability in vivo, but their cell number is limited. Current conventional culture conditions do not allow muscle stem cells to expand in vitro with their bona fide engraftment efficiency, requiring the improvement of culture procedures for achieving successful cell-therapy for muscle disorders. Here we expanded mouse muscle stem cells and human myoblasts with Notch ligands, DLL1, DLL4, and JAG1 to activate Notch signaling in vitro and to investigate whether these cells could retain their engraftment efficiency...
2017: PloS One
https://www.readbyqxmd.com/read/28491098/epigenetic-manipulation-facilitates-the-generation-of-skeletal-muscle-cells-from-pluripotent-stem-cells
#6
REVIEW
Tomohiko Akiyama, Shunichi Wakabayashi, Atsumi Soma, Saeko Sato, Yuhki Nakatake, Mayumi Oda, Miyako Murakami, Miki Sakota, Nana Chikazawa-Nohtomi, Shigeru B H Ko, Minoru S H Ko
Human pluripotent stem cells (hPSCs) have the capacity to differentiate into essentially all cell types in the body. Such differentiation can be directed to specific cell types by appropriate cell culture conditions or overexpressing lineage-defining transcription factors (TFs). Especially, for the activation of myogenic program, early studies have shown the effectiveness of enforced expression of TFs associated with myogenic differentiation, such as PAX7 and MYOD1. However, the efficiency of direct differentiation was rather low, most likely due to chromatin features unique to hPSCs, which hinder the access of TFs to genes involved in muscle differentiation...
2017: Stem Cells International
https://www.readbyqxmd.com/read/28489749/association-between-pax7-and-ntn1-gene-polymorphisms-and-nonsyndromic-orofacial-clefts-in-a-northern-chinese-population
#7
Qiang Guo, Dongmei Li, Xiangbiao Meng, Tingting Liu, Jinna Shi, Yanru Hao, Xiaohui Jiao, Kewen Lv, Tenglong Hu, Tao Song
BACKGROUND: Nonsyndromic orofacial clefts (NSOC) are the most common orofacial congenital defect with a complex etiology. Genome-wide association studies have identified paired box protein 7 (PAX7) and netrin-1 (NTN1) as candidate susceptibility genes for NSOC in both European and Asian populations. Here, possible associations between single-nucleotide polymorphisms (SNPs) in or near PAX7 and NTN1 were investigated in relation to risk of NSOC in a northern Chinese population. METHODS: A total of 602 individuals with NSOC and 510 controls were recruited from northern China...
May 2017: Medicine (Baltimore)
https://www.readbyqxmd.com/read/28464938/quiescence-of-human-muscle-stem-cells-is-favored-by-culture-on-natural-biopolymeric-films
#8
Claire Monge, Nicholas DiStasio, Thomas Rossi, Muriel Sébastien, Hiroshi Sakai, Benoit Kalman, Thomas Boudou, Shahragim Tajbakhsh, Isabelle Marty, Anne Bigot, Vincent Mouly, Catherine Picart
BACKGROUND: Satellite cells are quiescent resident muscle stem cells that present an important potential to regenerate damaged tissue. However, this potential is diminished once they are removed from their niche environment in vivo, prohibiting the long-term study and genetic investigation of these cells. This study therefore aimed to provide a novel biomaterial platform for the in-vitro culture of human satellite cells that maintains their stem-like quiescent state, an important step for cell therapeutic studies...
May 2, 2017: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/28453788/usage-of-dexamethasone-increases-the-risk-of-cranial-neural-crest-dysplasia-in-the-chick-embryo
#9
Xin Cheng, He Li, Yu Yan, Guang Wang, Zachary Berman, Manli Chuai, Xuesong Yang
Dexamethasone (Dex) is commonly used in the treatment of a variety of benign and malignant conditions. Unfortunately, although it has a variety of teratogenic effects, it remains used in clinical practice for pregnant women mainly due to limited alternatives. However, there is limited knowledge of the mechanisms that lead to the observed teratogenic effects. In this study, the effects of Dex during embryogenesis on neural crest development were evaluated in the early chick embryos. First, we demonstrated that 100µL 10-6 M Dex treatment leads to craniofacial developmental defects, and also retards embryo growth and plausibly can cause embryo demise...
April 27, 2017: Toxicological Sciences: An Official Journal of the Society of Toxicology
https://www.readbyqxmd.com/read/28441415/pax7-remodels-the-chromatin-landscape-in-skeletal-muscle-stem-cells
#10
Karin C Lilja, Nan Zhang, Alessandro Magli, Volkan Gunduz, Christopher J Bowman, Robert W Arpke, Radbod Darabi, Michael Kyba, Rita Perlingeiro, Brian D Dynlacht
Pluripotent stem cells (PSC) hold great promise for the treatment of human skeletal muscle diseases. However, it remains challenging to convert PSC to skeletal muscle cells, and the mechanisms by which the master regulatory transcription factor, Pax7, promotes muscle stem (satellite) cell identity are not yet understood. We have taken advantage of PSC-derived skeletal muscle precursor cells (iPax7), wherein the induced expression of Pax7 robustly initiates the muscle program and enables the in vitro generation of precursors that seed the satellite cell compartment upon transplantation...
2017: PloS One
https://www.readbyqxmd.com/read/28438836/a-wnt-notch-pax7-signaling-network-supports-tissue-integrity-in-tongue-development
#11
Xiao-Jing Zhu, Xueyan Yuan, Min Wang, Yukun Fang, Yudong Liu, Xiaoyun Zhang, Xueqin Yang, Yan Li, Jianying Li, Feixue Li, Zhong-Min Dai, Mengsheng Qiu, Ze Zhang, Zunyi Zhang
The tongue is one of the major structures involved in human food intake and speech. Tongue malformations such as aglossia, microglossia, and ankyloglossia are congenital birth defects, greatly affecting individuals' quality of life. However, the molecular basis of the tissue-tissue interactions that ensure tissue morphogenesis to form a functional tongue remains largely unknown. Here we show that ShhCre-mediated epithelial deletion of Wntless (Wls), the key regulator for intracellular Wnt trafficking, leads to lingual hypoplasia in mice...
April 24, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/28417015/characterization-and-isolation-of-highly-purified-porcine-satellite-cells
#12
Shijie Ding, Fei Wang, Yan Liu, Sheng Li, Guanghong Zhou, Ping Hu
Pig is an important food source and an excellent system to model human diseases. Careful characterization of the swine skeletal muscle stem cells (satellite cells) will shed lights on generation of swine skeletal muscle disease model and efficient production of porcine meat for the food industry. Paired box protein 7 (Pax7) is a highly conserved transcription factor shared by satellite cells from various species. However, the sequence of Pax7 has not been characterized in pig. The lack of method to isolate highly purified satellite cells hinders the thorough characterization of the swine satellite cells...
2017: Cell Death Discovery
https://www.readbyqxmd.com/read/28372522/skeletal-myoblast-seeded-vascularized-tissue-scaffolds-in-the-treatment-of-a-large-volumetric-muscle-defect-in-the-rat-biceps-femoris-muscle
#13
Mon Tzu Li, Marissa Ruehle, Hazel Stevens, Nick Servies, Nick Willett, Sukhita Karthikeyakannan, Gordon L Warren, Robert Guldberg, Laxminarayanan N Krishnan
High velocity impact injuries can often result in loss of large skeletal muscle mass, creating defects devoid of matrix, cells, and vasculature. Functional regeneration within these regions of large volumetric muscle loss (VML) continues to be a significant clinical challenge. Large cell-seeded, space-filling tissue engineered constructs that may augment regeneration require adequate vascularization to maintain cell viability. However, the long-term effect of improved vascularization and the effect of addition of myoblasts to vascularized constructs have not been determined in large VMLs...
April 3, 2017: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/28369879/human-skeletal-muscle-fibroblasts-stimulate-in-vitro-myogenesis-and-in-vivo-muscle-regeneration
#14
Abigail L Mackey, Mélanie Magnan, Bénédicte Chazaud, Michael Kjaer
Accumulation of skeletal muscle extracellular matrix is an unfavourable characteristic of many muscle diseases, muscle injury and sarcopenia. In addition to the indispensable role satellite cells play in muscle regeneration, there is emerging evidence in rodents for a regulatory influence on fibroblast activity. However the influence of fibroblasts on satellite cells and muscle regeneration in humans is unknown. The purpose of this study was to investigate this in vitro and during in vivo regeneration in humans...
March 30, 2017: Journal of Physiology
https://www.readbyqxmd.com/read/28355135/dynamic-transcriptional-signature-and-cell-fate-analysis-reveals-plasticity-of-individual-neural-plate-border-cells
#15
Daniela Roellig, Johanna Tan-Cabugao, Sevan Esaian, Marianne E Bronner
The 'neural plate border' of vertebrate embryos contains precursors of neural crest and placode cells, both defining vertebrate characteristics. How these lineages segregate from neural and epidermal fates has been a matter of debate. We address this by performing a fine-scale quantitative temporal analysis of transcription factor expression in the neural plate border of chick embryos. The results reveal significant overlap of transcription factors characteristic of multiple lineages in individual border cells from gastrula through neurula stages...
March 29, 2017: ELife
https://www.readbyqxmd.com/read/28344332/molecular-and-functional-heterogeneity-of-early-postnatal-porcine-satellite-cell-populations-is-associated-with-bioenergetic-profile
#16
Claudia Miersch, Katja Stange, Silvio Hering, Martin Kolisek, Torsten Viergutz, Monika Röntgen
During postnatal development, hyperplastic and hypertrophic processes of skeletal muscle growth depend on the activation, proliferation, differentiation, and fusion of satellite cells (SC). Therefore, molecular and functional SC heterogeneity is an important component of muscle plasticity and will greatly affect long-term growth performance and muscle health. However, its regulation by cell intrinsic and extrinsic factors is far from clear. In particular, there is only minor information on the early postnatal period which is critical for muscle maturation and the establishment of adult SC pools...
March 27, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28334989/impaired-fetal-muscle-development-and-jak-stat-activation-mark-disease-onset-and-progression-in-a-mouse-model-for-merosin-deficient-congenital-muscular-dystrophy
#17
Andreia M Nunes, Ryan D Wuebbles, Apurva Sarathy, Tatiana M Fontelonga, Marianne Deries, Dean J Burkin, Sólveig Thorsteinsdóttir
Merosin-deficient congenital muscular dystrophy type 1A (MDC1A) is a dramatic neuromuscular disease in which crippling muscle weakness is evident from birth. Here, we use the dyW mouse model for human MDC1A to trace the onset of the disease during development in utero. We find that myotomal and primary myogenesis proceed normally in homozygous dyW-/- embryos. Fetal dyW-/- muscles display the same number of myofibers as wildtype (WT) muscles, but by E18.5 dyW-/- muscles are significantly smaller and muscle size is not recovered post-natally...
June 1, 2017: Human Molecular Genetics
https://www.readbyqxmd.com/read/28282360/an-alpha7-nicotinic-acetylcholine-receptor-agonist-gts-21-promotes-c2c12-myonuclear-accretion-in-association-with-release-of-interleukin-6-il-6-and-improves-survival-in-burned-mice
#18
Mohammed A S Khan, Mohammed F Khan, Shizuka Kashiwagi, William R Kem, Shingo Yasuhara, Masao Kaneki, Ronald G Tompkins, J A Jeevendra Martyn
The role of interleukin-6 (IL-6) in physiological processes and disease is poorly understood. The hypothesis tested in this study was that selective alpha7 acetylcholine receptor (α7AChR) agonist, GTS-21, releases of IL-6 in association with myonuclear accretion and enhances insulin signaling in muscle cells, and improves survival of burn injured (BI) mice. The in vitro effects of GTS-21 were determined in C2C12 myoblasts and 7-day differentiated myotubes (myotubes). The in vivo effects of GTS-21 were tested in BI wild type (WT) and IL-6 knockout (IL6KO) mice...
March 9, 2017: Shock
https://www.readbyqxmd.com/read/28279710/satellite-like-cells-contribute-to-pax7-dependent-skeletal-muscle-repair-in-adult-zebrafish
#19
Michael A Berberoglu, Thomas L Gallagher, Zachary T Morrow, Jared C Talbot, Kimberly J Hromowyk, Inês M Tenente, David M Langenau, Sharon L Amacher
Satellite cells, also known as muscle stem cells, are responsible for skeletal muscle growth and repair in mammals. Pax7 and Pax3 transcription factors are established satellite cell markers required for muscle development and regeneration, and there is great interest in identifying additional factors that regulate satellite cell proliferation, differentiation, and/or skeletal muscle regeneration. Due to the powerful regenerative capacity of many zebrafish tissues, even in adults, we are exploring the regenerative potential of adult zebrafish skeletal muscle...
April 15, 2017: Developmental Biology
https://www.readbyqxmd.com/read/28255772/isolation-culture-and-biological-characteristics-of-multipotent-porcine-skeletal-muscle-satellite-cells
#20
Jinjuan Yang, Hao Liu, Kunfu Wang, Lu Li, Hongyi Yuan, Xueting Liu, Yingjie Liu, Weijun Guan
Skeletal muscle has a huge regenerative potential for postnatal muscle growth and repair, which mainly depends on a kind of muscle progenitor cell population, called satellite cell. Nowadays, the majority of satellite cells were obtained from human, mouse, rat and other animals but rarely from pig. In this article, the porcine skeletal muscle satellite cells were isolated and cultured in vitro. The expression of surface markers of satellite cells was detected by immunofluorescence and RT-PCR assays. The differentiation capacity was assessed by inducing satellite cells into adipocytes, myoblasts and osteoblasts...
March 2, 2017: Cell and Tissue Banking
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