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https://www.readbyqxmd.com/read/29793089/gold-and-gold-silver-alloy-nanoparticles-enhance-the-myogenic-differentiation-of-myoblasts-through-p38-mapk-signaling-pathway-and-promote-in-vivo-skeletal-muscle-regeneration
#1
Juan Ge, Kai Liu, Wen Niu, Mi Chen, Min Wang, Yumeng Xue, Chuanbo Gao, Peter X Ma, Bo Lei
Under the severe trauma condition, the skeletal muscles regeneration process is inhibited by forming fibrous scar tissues. Understanding the interaction between bioactive nanomaterials and myoblasts perhaps has important effect on the enhanced skeletal muscle tissue regeneration. Herein, we investigate the effect of monodispersed gold and gold-silver nanoparticles (AuNPs and Au-AgNPs) on the proliferation, myogenic differentiation and associated molecular mechanism of myoblasts (C2C12), as well as the in vivo skeletal muscle tissue regeneration...
May 18, 2018: Biomaterials
https://www.readbyqxmd.com/read/29780359/the-mitotic-and-metabolic-effects-of-phosphatidic-acid-in-the-primary-muscle-cells-of-turbot-scophthalmus-maximus
#2
Tingting Wang, Xuan Wang, Huihui Zhou, Haowen Jiang, Kangsen Mai, Gen He
Searching for nutraceuticals and understanding the underlying mechanism that promote fish growth is at high demand for aquaculture industry. In this study, the modulatory effects of soy phosphatidic acids (PA) on cell proliferation, nutrient sensing, and metabolic pathways were systematically examined in primary muscle cells of turbot ( Scophthalmus maximus ). PA was found to stimulate cell proliferation and promote G1/S phase transition through activation of target of rapamycin signaling pathway. The expression of myogenic regulatory factors, including myoD and follistatin , was upregulated, while that of myogenin and myostatin was downregulated by PA...
2018: Frontiers in Endocrinology
https://www.readbyqxmd.com/read/29753710/mysteries-of-host-switching-diversification-and-host-specificity-in-rodent-coccidia-associations
#3
Anna Mácová, Aneta Hoblíková, Václav Hypša, Michal Stanko, Jana Martinů, Jana Kvičerová
Recent studies show that host switching is much more frequent than originally believed and constitutes an important driver in evolution of host-parasite associations. However, its frequency and ecological mechanisms at the population level have been rarely investigated. We address this issue by analyzing phylogeny and population genetics of an extensive sample, from a broad geographic area, for commonly occurring parasites of the genus Eimeria within the abundant rodent genera Apodemus, Microtus and Myodes, using two molecular markers...
May 10, 2018: Molecular Phylogenetics and Evolution
https://www.readbyqxmd.com/read/29742392/direct-reprogramming-of-mouse-fibroblasts-into-functional-skeletal-muscle-progenitors
#4
Ori Bar-Nur, Mattia F M Gerli, Bruno Di Stefano, Albert E Almada, Amy Galvin, Amy Coffey, Aaron J Huebner, Peter Feige, Cassandra Verheul, Priscilla Cheung, Duygu Payzin-Dogru, Sylvain Paisant, Anthony Anselmo, Ruslan I Sadreyev, Harald C Ott, Shahragim Tajbakhsh, Michael A Rudnicki, Amy J Wagers, Konrad Hochedlinger
Skeletal muscle harbors quiescent stem cells termed satellite cells and proliferative progenitors termed myoblasts, which play pivotal roles during muscle regeneration. However, current technology does not allow permanent capture of these cell populations in vitro. Here, we show that ectopic expression of the myogenic transcription factor MyoD, combined with exposure to small molecules, reprograms mouse fibroblasts into expandable induced myogenic progenitor cells (iMPCs). iMPCs express key skeletal muscle stem and progenitor cell markers including Pax7 and Myf5 and give rise to dystrophin-expressing myofibers upon transplantation in vivo...
May 8, 2018: Stem Cell Reports
https://www.readbyqxmd.com/read/29730257/regulation-of-the-antioxidant-response-by-myod-transcriptional-coactivator-in-castration-resistant-prostate-cancer-cells
#5
Shun Zhang, Lin-Hu Li, Hong-Mei Qiao, Xue Yang, Liang Chen, Xiao-Hui Luo
OBJECTIVE: To reveal the potential role of the basic helix-loop-helix myogenic transcription regulator MyoD in the regulation of of castration-resistant prostate cancer (CRPC). METHODS: Expression levels of MyoD was assessed in PCa tissues using quantitative RT-PCR (RT-qPCR) and immunohistochemistry and in experimentally induced castration-resistant LNCaP/R cells using RT-qPCR and immunoblotting. Effect of MyoD knockdown on LNCaP/R cells progression was determined by assessing cell proliferation, apoptosis and colony formation rate...
May 3, 2018: Urology
https://www.readbyqxmd.com/read/29720576/efficient-exon-skipping-of-sgcg-mutations-mediated-by-phosphorodiamidate-morpholino-oligomers
#6
Eugene J Wyatt, Alexis R Demonbreun, Ellis Y Kim, Megan J Puckelwartz, Andy H Vo, Lisa M Dellefave-Castillo, Quan Q Gao, Mariz Vainzof, Rita C M Pavanello, Mayana Zatz, Elizabeth M McNally
Exon skipping uses chemically modified antisense oligonucleotides to modulate RNA splicing. Therapeutically, exon skipping can bypass mutations and restore reading frame disruption by generating internally truncated, functional proteins to rescue the loss of native gene expression. Limb-girdle muscular dystrophy type 2C is caused by autosomal recessive mutations in the SGCG gene, which encodes the dystrophin-associated protein γ-sarcoglycan. The most common SGCG mutations disrupt the transcript reading frame abrogating γ-sarcoglycan protein expression...
May 3, 2018: JCI Insight
https://www.readbyqxmd.com/read/29709011/muscle-wasting-in-osteoarthritis-model-induced-by-anterior-cruciate-ligament-transection
#7
Jordana Miranda de Souza Silva, Paulo Vinicius Gil Alabarse, Vivian de Oliveira Nunes Teixeira, Eduarda Correa Freitas, Francine Hehn de Oliveira, Rafael Mendonça da Silva Chakr, Ricardo Machado Xavier
This study aimed to investigate the molecular pathways involved in muscle wasting in an animal model of osteoarthritis (OA) induced by anterior cruciate ligament transection (ACLT) in rats. Reduction of protein syntheses, increased proteolysis and impaired muscle regeneration are important pathways related to muscle wasting, and myogenin, MyoD, myostatin and MuRF-1 are some of their markers. Female Wistar rats were allocated into two groups: OA (submitted to the ACLT) and SHAM (submitted to surgery without ACLT)...
2018: PloS One
https://www.readbyqxmd.com/read/29705144/brachylaima-asakawai-sp-nov-trematoda-brachylaimidae-a-rodent-intestinal-fluke-in-hokkaido-japan-with-a-finding-of-the-first-and-second-intermediate-hosts
#8
Minoru Nakao, Mizuki Sasaki, Tsukasa Waki, Jason L Anders, Hirotaka Katahira
In the 1970's and 1980's, an unknown species of the genus Brachylaima (Trematoda: Brachylaimidae) had been recorded from the intestines of Rattus norvegicus and Apodemus speciosus in Hokkaido, Japan. The rodent fluke was characteristic in extending a bilateral vitellarium till the level of posterior margin of anterior testis and in keeping almost the same-sized spherical ovary and testes. In this study, the rodent fluke was rediscovered from A. speciosus, Apodemus argenteus, and Myodes rufocanus in Hokkaido...
April 26, 2018: Parasitology International
https://www.readbyqxmd.com/read/29704204/tissue-trace-elements-and-lipid-peroxidation-in-breeding-female-bank-voles-myodes-glareolus
#9
Elżbieta Bonda-Ostaszewska, Tadeusz Włostowski, Barbara Łaszkiewicz-Tiszczenko
Recent studies have demonstrated that reproduction reduces oxidative damage in various tissues of small mammal females. The present work was designed to determine whether the reduction of oxidative stress in reproductive bank vole females was associated with changes in tissue trace elements (iron, copper, zinc) that play an essential role in the production of reactive oxygen species. Lipid peroxidation (a marker of oxidative stress) and iron concentration in liver, kidneys, and skeletal muscles of reproducing bank vole females that weaned one litter were significantly lower than in non-reproducing females; linear regression analysis confirmed a positive relation between the tissue iron and lipid peroxidation...
April 27, 2018: Biological Trace Element Research
https://www.readbyqxmd.com/read/29687385/myogenic-differentiation-of-ascs-using-biochemical-and-biophysical-induction
#10
Pinar Yilgor Huri, Justin Morrissette-McAlmon, Warren L Grayson
Adipose-derived stem/stromal cells (ASCs) constitute a very promising source for cell therapy and tissue engineering approaches as they can be easily obtained in large quantities with comparatively minimal patient discomfort. Moreover, ASCs have multilineage differentiation capacity. Among these, differentiation capacity along the myogenic lineage is of particular interest since myogenic precursors are scarce and obtaining a large number of cells from skeletal muscle biopsies is difficult. Here, we describe a method to effectively induce ASC myogenesis through the combination of biochemical (cocktail including 5-azacytidine and horse serum) and biophysical (dynamic culture via uniaxial cyclic strain) stimulation...
2018: Methods in Molecular Biology
https://www.readbyqxmd.com/read/29680376/bisphenol-a-and-estradiol-impede-myoblast-differentiation-through-down-regulating-akt-signaling-pathway
#11
Ga-Yeon Go, Sang-Jin Lee, Ayoung Jo, Jae-Rin Lee, Jong-Sun Kang, Mihi Yang, Gyu-Un Bae
Bisphenol A (BPA), one of the most widespread endocrine disrupting chemicals, is known as an artificial estrogen, which interacts with estrogen receptor (ER). In this study, we investigated the effects of BPA and estradiol on myoblast differentiation and the underlying signaling mechanism. Exposure to BPA (0.01-1 μM) in mouse myoblast C2C12 cells attenuated myogenic differentiation via the reduced expression of muscle-specific genes, such as myosin heavy chain (MHC), MyoD, and Myogenin, without the alteration of cell proliferation and viability...
April 19, 2018: Toxicology Letters
https://www.readbyqxmd.com/read/29670434/small-terrestrial-mammals-of-albania-distribution-and-diversity-mammalia-eulipotyphla-rodentia
#12
Ferdinand Bego, Enerit Saçdanaku, Michela Pacifici, Carlo Rondinini
In this paper new records are reported for 23 species of small terrestrial mammals (STM) of Albania collected during the field work campaigns organised in the framework of the project "Strengthening capacity in National Nature Protection - preparation for Natura 2000 network" (NaturAL) in Albania during the summer and autumn of 2016 and 2017 Data on small mammals were primarily collected through Sherman live-trapping campaigns in six high priority protected areas of Albania: Korab-Koritnik, Bredhi i Hotovës, Tomorri, Llogara-Karaburun, Divjakë-Karavasta, Liqeni i Shkodrës (Skadar lake), Lëpushë-Vermosh...
2018: ZooKeys
https://www.readbyqxmd.com/read/29669436/heme-oxygenase-1-influences-satellite-cells-and-progression-of-duchenne-muscular-dystrophy-in-mice
#13
Katarzyna Pietraszek-Gremplewicz, Magdalena Kozakowska, Iwona Bronisz-Budzynska, Maciej Ciesla, Olga Mucha, Paulina Podkalicka, Magdalena Madej, Urszula Glowniak, Krzysztof Tomasz Szade, Jacek Stepniewski, Mateusz Jez, Kalina Andrysiak, Karolina Bukowska-Strakova, Anna Kaminska, Anna Kostera-Pruszczyk, Alicja Jozkowicz, Agnieszka Loboda, Jozef Dulak
AIMS: Muscle damage in Duchenne Muscular Dystrophy (DMD) caused by the lack of dystrophin is strongly linked to inflammation. Heme oxygenase-1 (HO-1; Hmox1) is an anti-inflammatory and cytoprotective enzyme affecting myoblasts differentiation by inhibiting myomirs. The role of HO-1 has not been so far well addressed in DMD. RESULTS: In dystrophin-deficient mdx mice expression of Hmox1 in limb skeletal muscles and diaphragm is higher than in wild-type animals, being consistently elevated from 8 up to 52 weeks, both in myofibres and inflammatory leukocytes...
April 19, 2018: Antioxidants & Redox Signaling
https://www.readbyqxmd.com/read/29651539/skeletal-muscle-stem-cell-characteristics-and-myonuclei-content-in-patients-with-rheumatoid-arthritis-a-cross-sectional-study
#14
Rasmus Jentoft Boutrup, Jean Farup, Kristian Vissing, Michael Kjaer, Ulla Ramer Mikkelsen
To investigate satellite cells (SCs) and myonuclei characteristics in patients with rheumatoid arthritis (RA). Resting biopsies from m. vastus lateralis were obtained from thirteen RA patients and thirteen matched healthy controls (CON). Muscle biopsies were immunohistochemically stained and analyzed for fiber type specific content of SCs (Pax7+ ), proliferating SCs (Pax7+ /MyoD+ ) and differentiating SCs (myogenin+ ). Furthermore, we quantified fiber type specific content of myonuclei and myofiber cross-sectional area (CSA)...
April 12, 2018: Rheumatology International
https://www.readbyqxmd.com/read/29648516/the-phosphodiesterase-4-inhibitor-roflumilast-reverts-proteolysis-in-skeletal-muscle-cells-of-patients-with-copd-cachexia
#15
Esther Barreiro, Ester Puig-Vilanova, Anna Salazar-Degracia, Sergi Pascual-Guardia, Carme Casadevall, Joaquim Gea
Peripheral muscle weakness and mass loss are characteristic features in severe COPD. We hypothesized that the phosphodiesterase-4 inhibitor roflumilast-induced cAMP may ameliorate proteolysis and metabolism in skeletal muscles of COPD patients with severe muscle wasting. In myogenic precursor cells (isolated from muscle biopsies and cultured up to obtain differentiated myotubes) from 10 severe COPD patients and 10 healthy controls, which were treated with 1 microM roflumilast N-oxide (RNO) for three time-cohorts (1h, 6h, and 24h), genes of antioxidant defense and oxidative stress marker, myogenesis and muscle metabolism, proteolysis (tyrosine release assay) and ubiquitin-proteasome system markers, autophagy, and myosin isoforms were analyzed using RT-PCR and immunoblotting...
April 12, 2018: Journal of Applied Physiology
https://www.readbyqxmd.com/read/29628461/-molecular-mechanism-for-et-1-induced-insulin-resistance-in-skeletal-muscle-cells
#16
Takahiro Horinouchi, Yuichi Mazaki, Koji Terada, Soichi Miwa
Insulin resistance is a condition where the sensitivity to insulin of the tissues expressing insulin receptor (InsR) is decreased due to a functional disturbance of InsR-mediated intracellular signaling. Insulin promotes the entry of glucose into the tissues and skeletal muscle is the most important tissue responsible for the insulin's action of decreasing blood glucose levels. Endothelin-1 (ET-1), a potent vasoconstrictor and pro-inflammatory peptide, induces insulin resistance through a direct action on skeletal muscle...
2018: Nihon Yakurigaku Zasshi. Folia Pharmacologica Japonica
https://www.readbyqxmd.com/read/29627754/long-term-dim-light-during-nighttime-changes-activity-patterns-and-space-use-in-experimental-small-mammal-populations
#17
Julia Hoffmann, Rupert Palme, Jana Anja Eccard
Artificial light at night (ALAN) is spreading worldwide and thereby is increasingly interfering with natural dark-light cycles. Meanwhile, effects of very low intensities of light pollution on animals have rarely been investigated. We explored the effects of low intensity ALAN over seven months in eight experimental bank vole (Myodes glareolus) populations in large grassland enclosures over winter and early breeding season, using LED garden lamps. Initial populations consisted of eight individuals (32 animals per hectare) in enclosures with or without ALAN...
April 5, 2018: Environmental Pollution
https://www.readbyqxmd.com/read/29626795/growth-hormone-facilitates-5-azacytidine-induced-myogenic-but-inhibits-5-azacytidine-induced-adipogenic-commitment-in-c3h10t1-2-mesenchymal-stem-cells
#18
Dan Jia, Weijiang Zheng, Honglin Jiang
The C3H10T1/2 cells are considered mesenchymal stem cells (MSCs) because they can be induced to become the progenitor cells for myocytes, adipocytes, osteoblasts, and chondrocytes by the DNA methyltransferase inhibitor 5'-azacytidine. In this study, we determined the effect of growth hormone (GH) on the myogenic and adipogenic lineage commitment in C3H10T1/2 cells. The C3H10T1/2 cells were treated with recombinant bovine GH in the presence or absence of 5'-azacytidine for 4 days. The myogenic commitment in C3H10T1/2 cells was assessed by immunostaining them for MyoD, the marker for myoblasts, and by determining their capacity to differentiate into the multinucleated myotubes...
March 28, 2018: Growth Hormone & IGF Research
https://www.readbyqxmd.com/read/29625618/exposure-to-radial-extracorporeal-shock-waves-modulates-viability-and-gene-expression-of-human-skeletal-muscle-cells-a-controlled-in-vitro-study
#19
Stefan G Mattyasovszky, Eva K Langendorf, Ulrike Ritz, Christoph Schmitz, Irene Schmidtmann, Tobias E Nowak, Daniel Wagner, Alexander Hofmann, Pol M Rommens, Philipp Drees
BACKGROUND: Recent clinical and animal studies have shown that extracorporeal shock wave therapy has a promoting influence on the healing process of musculoskeletal disorders. However, the underlying biological effects of extracorporeal shock wave therapy on human skeletal muscle cells have not yet been investigated. METHODS: In this study, we investigated human skeletal muscle cells after exposure to radial extracorporeal shock waves in a standardized in vitro setup...
April 6, 2018: Journal of Orthopaedic Surgery and Research
https://www.readbyqxmd.com/read/29618024/a-novel-long-non-coding-rna-myolinc-regulates-myogenesis-through-tdp-43-and-filip1
#20
Giuseppe Militello, Mohammed Rabiul Hosen, Yuliya Ponomareva, Pascal Gellert, Tyler Weirick, David John, Sajedah Mahmoud Hindi, Kamel Mamchaoui, Vincent Mouly, Claudia Döring, Lidan Zhang, Miki Nakamura, Ashok Kumar, So-Ichiro Fukada, Stefanie Dimmeler, Shizuka Uchida
Myogenesis is a complex process required for skeletal muscle formation during embryonic development and for regeneration and growth of myofibers in adults. Accumulating evidence suggests that long non-coding RNAs (lncRNAs) play key roles in regulating cell fate decision and function in various tissues. However, the role of lncRNAs in the regulation of myogenesis remains poorly understood. In this study, we identified a novel muscle-enriched lncRNA called "Myolinc (AK142388)", which we functionally characterized in the C2C12 myoblast cell line...
April 3, 2018: Journal of Molecular Cell Biology
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