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https://www.readbyqxmd.com/read/29333723/nandrolone-induced-nuclear-accumulation-of-myod-protein-is-mediated-by-numb-a-notch-inhibitor-in-c2c12-myoblasts
#1
Xin-Hua Liu, Rita De Gasperi, William A Bauman, Christopher P Cardozo
Signaling via the androgen receptor (AR) stimulates myogenic progenitor differentiation. In addition, myogenic differentiation factor D (MyoD) and Numb, a Notch inhibitor, play key roles in regulating myogenic differentiation. Nandrolone, an anabolic steroid, upregulates both MyoD and Numb expression in myogenic cells. However, the molecular mechanisms by which MyoD is upregulated by nandrolone are unclear. Moreover, the potential crosstalk between nandrolone, MyoD, and Numb is not well understood. With these considerations in mind, we examined the effects of nandrolone on the expression of MyoD mRNA and protein, and determined the interactions of MyoD and Numb in the presence or absence of nandrolone in differentiating C2C12 myoblasts...
January 2018: Physiological Reports
https://www.readbyqxmd.com/read/29333581/high-final-energy-of-gallium-arsenide-laser-increases-myod-gene-expression-during-the-intermediate-phase-of-muscle-regeneration-after-cryoinjury-in-rats
#2
Caroline Pereira Santos, Andreo Fernando Aguiar, Ines Cristina Giometti, Thaoan Bruno Mariano, Carlos Eduardo Assumpção de Freitas, Gisele Alborghetti Nai, Selma Zambelli de Freitas, Maeli Dal Pai-Silva, Francis Lopes Pacagnelli
The aim of this study was to determine the effects of gallium arsenide (GaAs) laser on IGF-I, MyoD, MAFbx, and TNF-α gene expression during the intermediate phase of muscle regeneration after cryoinjury 21 Wistar rats were divided into three groups (n = 7 per group): untreated with no injury (control group), cryoinjury without GaAs (injured group), and cryoinjury with GaAs (GaAs-injured group). The cryoinjury was induced in the central region of the tibialis anterior muscle (TA). The region injured was irradiated once a day during 14 days using GaAs laser (904 nm; spot size 0...
January 15, 2018: Lasers in Medical Science
https://www.readbyqxmd.com/read/29328071/sumoylation-of-histone-deacetylase-1-regulates-myod-signaling-during-myogenesis
#3
Hosouk Joung, Sehee Kwon, Kyoung-Hoon Kim, Yun-Gyeong Lee, Sera Shin, Duk-Hwa Kwon, Yeong-Un Lee, Taewon Kook, Nakwon Choe, Jeong Chul Kim, Young-Kook Kim, Gwang Hyeon Eom, Hyun Kook
Sumoylation, the conjugation of a small ubiquitin-like modifier (SUMO) protein to a target, has diverse cellular effects. However, the functional roles of the SUMO modification during myogenesis have not been fully elucidated. Here, we report that basal sumoylation of histone deacetylase 1 (HDAC1) enhances the deacetylation of MyoD in undifferentiated myoblasts, whereas further sumoylation of HDAC1 contributes to switching its binding partners from MyoD to Rb to induce myocyte differentiation. Differentiation in C2C12 skeletal myoblasts induced new immunoblot bands above HDAC1 that were gradually enhanced during differentiation...
January 12, 2018: Experimental & Molecular Medicine
https://www.readbyqxmd.com/read/29318853/-effects-of-electroacupuncture-on-adaptive-hypertrophy-of-gastrocnemius-and-proliferation-and-differentiation-of-muscle-satellite-cells-in-rats
#4
Dan-Dan Zhao, Cheng-Lin Tang, Si-Qin Huang, Ao Luo, An-Ning Zhang, Quan-Hu Guo, Jing Cao, Rui-Qi Gao
OBJECTIVE: To explore the effects and mechanism of different duration electroacupuncture (EA) on adaptive hypertrophy of gastrocnemius and the expressions of proliferation cell nuclear antigen (PCNA), paired box transcription factor 7 (Pax 7), myogenic differentiation antigen (MyoD 1), myogenin (MyoG), myosin heavy chain-Ⅰ(Myh 7), transforming growth factor-β 1 (TGF-β 1), Smad 3 in rats. METHODS: Twenty-four SD adult male rats were randomly divided into normal, two-week EA (A 2), four-week EA (A 4) and eight-week EA (A 8) groups, 6 rats in each group...
December 25, 2017: Zhen Ci Yan Jiu, Acupuncture Research
https://www.readbyqxmd.com/read/29316239/distribution-and-space-use-of-seed-dispersing-rodents-in-central-pyrenees-implications-for-genetic-diversity-conservation-and-plant-recruitment
#5
Jon Urgoiti, Alberto Muñoz, Josep Maria Espelta, Raúl Bonal
The function and conservation of many forest ecosystems depend on the distribution and diversity of the community of rodents that consume and disperse seeds. The habitat preferences and interactions are especially relevant in alpine systems where such granivorous rodents reach the southernmost limit of their distribution and are especially sensitive to global warming. We analysed the community of granivorous rodents in the Pyrenees, one of the southernmost mountain ranges of Europe. Rodent species were identified by DNA with particular attention to the Apodemus species, which are prominent seed-dispersing rodents in Europe...
January 9, 2018: Integrative Zoology
https://www.readbyqxmd.com/read/29315567/the-influence-of-capillarization-on-satellite-cell-pool-expansion-and-activation-following-exercise-induced-muscle-damage-in-healthy-young-men
#6
Joshua P Nederveen, Sophie Joanisse, Tim Snijders, Aaron Cq Thomas, Dinesh Kumbhare, Gianni Parise
Factors that determine the skeletal muscle satellite cell (SC) response remain incompletely understood. It is known, however, that SC activation status is closely related to the anatomical relationship between SC and muscle capillaries. We investigated the impact of muscle fibre capillarization on the expansion and activation status of SC following a muscle damaging exercise protocol in healthy young men. Twenty-nine young men (21 ± 0.5 yrs) performed 300 unilateral eccentric contractions (180 deg·s-1 ) of the knee extensors...
January 6, 2018: Journal of Physiology
https://www.readbyqxmd.com/read/29313468/molecular-epidemiology-of-an-orientia-tsutsugamushi-gene-encoding-a-56-kda-type-specific-antigen-in-chiggers-small-mammals-and-patients-from-the-southwest-region-of-korea
#7
Jung Wook Park, Sun Hee Kim, Duck Woong Park, So Hyang Jung, Hye Jung Park, Mi Hee Seo, Hyeon Je Song, Jung Yoon Lee, Dong Min Kim, Choon Mi Kim, Byong Chul Gill, Hang Jin Jeong, Jeong Min Lee, Dong Ryong Ha, Eun Sun Kim, Jae Keun Chung
A phylogenetic analysis of Orientia tsutsugamushi was performed to elucidate its antigenic diversity in chiggers, small mammals, and patients. Between September 2014 and December 2016, a total of 3,816 chiggers were identified within nine species of four genera in the southwest region of Korea: Leptotrombidium scutellare (49.9%; 1,907/3,816), Leptotrombidium orientale (21.1%; 804/3,816), Leptotrombidium pallidum (12.4%; 474/3,816), Euchoengastia koreaensis (7.2%; 273/3,816), Leptotrombidium palpale (6.7%; 256/3,816), Neotrombicular gardellai (1...
January 8, 2018: American Journal of Tropical Medicine and Hygiene
https://www.readbyqxmd.com/read/29307818/characterization-of-the-promoter-region-of-bovine-six4-roles-of-e-box-and-myod-in-the-regulation-of-basal-transcription
#8
Dawei Wei, Laishun Feng, Wenzhen Zhang, Xueyao Ma, Gong Cheng, Shijun Li, Li Wang, Song Zhang, Jieyun Hong, Hongfang Guo, Yaning Wang, Yue Ning, Linsen Zan
The sine oculis homeobox 4 (SIX4) gene belongs to the Six gene family and encodes an evolutionarily conserved transcription factor. Previous studies have demonstrated that SIX4 plays an essential role in proper muscle regeneration. However, the mechanisms regulating SIX4 transcription remain elusive. In the present study, we determined that bovine SIX4 was highly expressed in the longissimus thoracis and in undifferentiated Qinchuan cattle muscle cells (QCMCs) and that its protein localizes to both the cytoplasm and the nucleus...
January 4, 2018: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/29307280/myogenic-regulatory-factors-the-orchestrators-of-myogenesis-after-30-years-of-discovery
#9
Hasan A Asfour, Mohammed Z Allouh, Raed S Said
Prenatal and postnatal myogenesis share many cellular and molecular aspects. Myogenic regulatory factors are basic Helix-Loop-Helix transcription factors that indispensably regulate both processes. These factors (Myf5, MyoD, Myogenin, and MRF4) function as an orchestrating cascade, with some overlapped actions. Prenatally, myogenic regulatory factors are restrictedly expressed in somite-derived myogenic progenitor cells and their derived myoblasts. Postnatally, myogenic regulatory factors are important in regulating the myogenesis process via satellite cells...
January 1, 2018: Experimental Biology and Medicine
https://www.readbyqxmd.com/read/29299217/a-mini-review-of-tat-myod-fused-proteins-state-of-the-art-and-problems-to-solve
#10
Marco Patruno, Luca Melotti, Chiara Gomiero, Roberta Sacchetto, Ohad Topel, Tiziana Martinello
The transcriptional activator TAT is a small peptide essential for viral replication and possesses the property of entering the cells from the extracellular milieu, acting as a membrane shuttle. In order to safely differentiate cells an innovative methodology, based on the fusion of transcription factors and the TAT sequence, is discussed in this short review. In several studies, it has been demonstrated that TAT protein can be observed in the cell nucleus after few hours from the inoculation although its way of action is not fully understood...
December 5, 2017: European Journal of Translational Myology
https://www.readbyqxmd.com/read/29298131/a-systematic-review-of-p53-regulation-of-oxidative-stress-in-skeletal-muscle
#11
Kaitlyn Beyfuss, David A Hood
BACKGROUND: p53 is a tumor suppressor protein involved in regulating a wide array of signaling pathways. The role of p53 in the cell is determined by the type of imposed oxidative stress, its intensity and duration. The last decade of research has unravelled a dual nature in the function of p53 in mediating the oxidative stress burden. However, this is dependent on the specific properties of the applied stress and thus requires further analysis. METHODS: A systematic review was performed following an electronic search of Pubmed, Google Scholar, and ScienceDirect databases...
January 3, 2018: Redox Report: Communications in Free Radical Research
https://www.readbyqxmd.com/read/29273298/recruitment-and-therapeutic-application-of-macrophages-in-skeletal-muscles-after-hind-limb-ischemia
#12
Pei-Ling Hsieh, Viktoriya Rybalko, Aaron B Baker, Laura J Suggs, Roger P Farrar
OBJECTIVE: Peripheral arterial disease can cause not only ischemia but also skeletal muscle damage. It has been known that macrophages (MPs) play an important role in coordinating muscle repair; however, phenotype transition of monocyte-MP in ischemic muscle has not been well defined. Hence, the purpose of this study was to examine the temporal recruitment of MPs and to explore their therapeutic effect on ischemic muscle regeneration. METHODS: Unilateral femoral artery excision was performed on C57BL/6 mice...
December 19, 2017: Journal of Vascular Surgery
https://www.readbyqxmd.com/read/29260008/functional-validation-and-expression-analysis-of-myotubes-converted-from-skin-fibroblasts-using-a-simple-direct-reprogramming-strategy
#13
Fukuko Horio, Hidetoshi Sakurai, Yutaka Ohsawa, Shiho Nakano, Makoto Matsukura, Isao Fujii
Previously, we reported that MyoD, a master gene for myogenic cells, could efficiently convert primary skin fibroblasts into myoblasts and myotubes, thereby effecting direct reprogramming. In this study, we further demonstrated that MyoD-expressing primary fibroblasts displayed rapid movement in culture, with a movement velocity that was significantly faster, almost four times, than mouse primary myoblasts. MyoD-transduced cells obtained the characteristics of Ca2 + release and electrically-stimulated contraction, which was comparable to C2C12 myotubes, suggesting that the essential features of muscle were observed in the transduced cells...
March 2017: ENeurologicalSci
https://www.readbyqxmd.com/read/29258243/canadine-from-corydalis-turtschaninovii-stimulates-myoblast-differentiation-and-protects-against-myotube-atrophy
#14
Heyjin Lee, Sang-Jin Lee, Gyu-Un Bae, Nam-In Baek, Jae-Ha Ryu
Cachexia and sarcopenia are the main causes of muscle atrophy. These result in a reduction in the muscle fiber area, myo-protein content, and muscle strength, with various molecular modulators being involved. Although several reports have proposed potential therapeutic agents, no effective treatments have been found for muscle atrophy. We searched for myogenic modulators from medicinal plants to treat muscle diseases. We isolated six alkaloids from Corydalis turtschaninovii and evaluated their myogenic potential by using the MyoD reporter gene assay in C2C12 cells...
December 18, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/29245050/nanog-restores-the-impaired-myogenic-differentiation-potential-of-skeletal-myoblasts-after-multiple-population-doublings
#15
Aref Shahini, Debanik Choudhury, Mohammadnabi Asmani, Ruogang Zhao, Pedro Lei, Stelios T Andreadis
Adult skeletal muscle regeneration relies on the activity of satellite cells residing in the skeletal muscle niche. However, systemic and intrinsic factors decrease the myogenic differentiation potential of satellite cells thereby impairing muscle regeneration. Here we present data showing that late passage C2C12 myoblasts exhibited significantly impaired myogenic differentiation potential that was accompanied by impaired expression of myogenic regulatory factors (Myf5, MyoD, Myogenin, and MRF4) and members of myocyte enhancer factor 2 family...
December 2, 2017: Stem Cell Research
https://www.readbyqxmd.com/read/29242210/reversible-immortalisation-enables-genetic-correction-of-human-muscle-progenitors-and-engineering-of-next-generation-human-artificial-chromosomes-for-duchenne-muscular-dystrophy
#16
Sara Benedetti, Narumi Uno, Hidetoshi Hoshiya, Martina Ragazzi, Giulia Ferrari, Yasuhiro Kazuki, Louise Anne Moyle, Rossana Tonlorenzi, Angelo Lombardo, Soraya Chaouch, Vincent Mouly, Marc Moore, Linda Popplewell, Kanako Kazuki, Motonobu Katoh, Luigi Naldini, George Dickson, Graziella Messina, Mitsuo Oshimura, Giulio Cossu, Francesco Saverio Tedesco
Transferring large or multiple genes into primary human stem/progenitor cells is challenged by restrictions in vector capacity, and this hurdle limits the success of gene therapy. A paradigm is Duchenne muscular dystrophy (DMD), an incurable disorder caused by mutations in the largest human gene: dystrophin. The combination of large-capacity vectors, such as human artificial chromosomes (HACs), with stem/progenitor cells may overcome this limitation. We previously reported amelioration of the dystrophic phenotype in mice transplanted with murine muscle progenitors containing a HAC with the entire dystrophin locus (DYS-HAC)...
December 14, 2017: EMBO Molecular Medicine
https://www.readbyqxmd.com/read/29241530/atp-citrate-lyase-regulates-myofiber-differentiation-and-increases-regeneration-by-altering-histone-acetylation
#17
Suman Das, Frederic Morvan, Giulio Morozzi, Benjamin Jourde, Giulia C Minetti, Peter Kahle, Helene Rivet, Pascale Brebbia, Gauthier Toussaint, David J Glass, Mara Fornaro
ATP citrate lyase (ACL) plays a key role in regulating mitochondrial function, as well as glucose and lipid metabolism in skeletal muscle. We report here that ACL silencing impairs myoblast and satellite cell (SC) differentiation, and it is accompanied by a decrease in fast myosin heavy chain isoforms and MYOD. Conversely, overexpression of ACL enhances MYOD levels and promotes myogenesis. Myogenesis is dependent on transcriptional but also other mechanisms. We show that ACL regulates the net amount of acetyl groups available, leading to alterations in acetylation of H3(K9/14) and H3(K27) at the MYOD locus, thus increasing MYOD expression...
December 12, 2017: Cell Reports
https://www.readbyqxmd.com/read/29237850/stabilizing-selection-on-microsatellite-allele-length-at-arginine-vasopressin-1a-receptor-and-oxytocin-receptor-loci
#18
Phillip C Watts, Eva R Kallio, Esa Koskela, Eija Lonn, Tapio Mappes, Mikael Mokkonen
The loci arginine vasopressin receptor 1a (avpr1a) and oxytocin receptor (oxtr) have evolutionarily conserved roles in vertebrate social and sexual behaviour. Allelic variation at a microsatellite locus in the 5' regulatory region of these genes is associated with fitness in the bank vole Myodes glareolus Given the low frequency of long and short alleles at these microsatellite loci in wild bank voles, we used breeding trials to determine whether selection acts against long and short alleles. Female bank voles with intermediate length avpr1a alleles had the highest probability of breeding, while male voles whose avpr1a alleles were very different in length had reduced probability of breeding...
December 20, 2017: Proceedings. Biological Sciences
https://www.readbyqxmd.com/read/29236296/rna-decay-factor-upf1-promotes-protein-decay-a-hidden-talent
#19
REVIEW
Terra-Dawn M Plank, Miles F Wilkinson
The RNA-binding protein, UPF1, is best known for its central role in the nonsense-mediated RNA decay (NMD) pathway. Feng et al. now report a new function for UPF1-it is an E3 ubiquitin ligase that specifically promotes the decay of a key pro-muscle transcription factor: MYOD. UPF1 achieves this through its RING-like domain, which confers ubiquitin E3 ligase activity. Feng et al. provide evidence that the ability of UPF1 to destabilize MYOD represses myogenesis. In the future, it will be important to define other protein substrates of UPF1-driven ubiquitination and to determine whether this biochemical activity is responsible for some of UPF1's previously defined biological functions, including in development and stress responses...
December 13, 2017: BioEssays: News and Reviews in Molecular, Cellular and Developmental Biology
https://www.readbyqxmd.com/read/29224337/spermidine-activated-satellite-cells-are-associated-with-hypoacetylation-in-acvr2b-and-smad3-binding-to-myogenic-genes-in-mice
#20
Luchu Zhang, Huiying Gong, Qinwei Sun, Ruqian Zhao, Yimin Jia
Spermidine is an acetyltransferase inhibitor and a specific inducer of autophagy. Recently, spermidine is identified as a potential therapeutic agent for age-related muscle atrophy and inherited myopathies. However, the effect of spermidine on non-pathological skeletal muscle remains unclear. In this study, long-term spermidine administration in mice lowered the mean cross-sectional area of the gastrocnemius muscle, and reduced the expression of myosin heavy chain isoforms in the muscle, which was associated with ubiquitination...
December 10, 2017: Journal of Agricultural and Food Chemistry
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