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https://www.readbyqxmd.com/read/28725668/data-on-myod-reduction-by-autophagy-in-c2c12-cells
#1
Yeong-Min Yoo, Yung Chul Park
Autophagy is a highly regulated physiologic mechanism in which cells maintain homeostasis by degrading excessive or unnecessary proteins and damaged or aged organelles through the lysosomal machinery (Yorimitsu and Klionsky, 2005) [1]. MyoD is basic helix-loop-helix (bHLH) transcription factors that regulate myoblast proliferation and myogenic differentiation. MyoD is expressed in adult skeletal muscle (Megeney et al., 1996) [2] and adult fibers (Brack et al., 2005) [3]. MyoD is mainly degraded by the ubiquitin-proteasome system (Floyd et al...
August 2017: Data in Brief
https://www.readbyqxmd.com/read/28725008/methylglyoxal-and-advanced-glycation-end-products-insight-of-the-regulatory-machinery-affecting-the-myogenic-program-and-of-its-modulation-by-natural-compounds
#2
Mohammad Hassan Baig, Arif Tasleem Jan, Gulam Rabbani, Khurshid Ahmad, Jalaluddin M Ashraf, Taeyeon Kim, Han Sol Min, Yong Ho Lee, Won-Kyung Cho, Jin Yeul Ma, Eun Ju Lee, Inho Choi
Methylglyoxal (MG) is a reactive dicarbonyl intermediate and a precursor of advanced glycation end products (AGEs). The authors investigated the role played by AGEs in muscle myopathy and the amelioration of its effects by curcumin and gingerol. In addition to producing phenotypical changes, MG increased oxidative stress and reduced myotube formation in C2C12 cells. RAGE (receptor for AGEs) expression was up-regulated and MYOD and myogenin (MYOG) expressions were concomitantly down-regulated in MG-treated cells...
July 19, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28721121/does-the-injection-of-platelet-rich-plasma-induce-changes-in-the-gene-expression-and-morphology-of-intact-thoroughbred-skeletal-muscle
#3
Kentaro Fukuda, Hirofumi Miyata, Atsutoshi Kuwano, Taisuke Kuroda, Norihisa Tamura, Yasumitsu Kotoyori, Yoshinori Kasashima
Platelet-rich plasma (PRP) therapy is promising for treating skeletal muscle injuries in human athletes by promoting muscle regeneration. It might also be useful for treating muscle injuries in equine athletes. In the present study, muscle regeneration induced by injection of PRP into intact muscle of Thoroughbred was investigated. Autologous PRP and saline were injected twice into intact left and right gluteus medius muscles of seven clinically healthy Thoroughbreds. Muscle samples were collected from the injection sites by needle biopsy at 2 and 7 days after PRP injection...
2017: Journal of Equine Science
https://www.readbyqxmd.com/read/28707296/ebp1-regulates-myogenic-differentiation-of-myoblast-cells-via-smad2-3-signaling-pathway
#4
Minli Yu, Huan Wang, Zhen Liu, Yinglin Lu, Debing Yu, Dongfeng Li, Wenxing Du
Regulation of skeletal muscle development requires many of the regulatory networks that are fundamental to developmental myogenesis. ErbB3 binding protein-1 (Ebp1) is involved in the control of myoblasts development in chicken. However, the expression and biological functions of Ebp1 in the progress of myogenesis are unclear. This study focused on determining the effect of Ebp1 on myogenic proliferation and differentiation using a primary myoblasts culture model. Ebp1 was found to upregulate in proliferating myoblasts and decrease at the early stage of myogenic differentiation...
July 13, 2017: Development, Growth & Differentiation
https://www.readbyqxmd.com/read/28697464/laminin-111-enriched-fibrin-hydrogels-for-skeletal-muscle-regeneration
#5
Madison Marcinczyk, Hady Elmashhady, Muhamed Talovic, Andrew Dunn, Faiz Bugis, Koyal Garg
Laminin (LM)-111 supplementation has improved muscle regeneration in several models of disease and injury. This study investigated a novel hydrogel composed of fibrinogen and LM-111. Increasing LM-111 concentration (50-450 μg/mL) in fibrin hydrogels resulted in highly fibrous scaffolds with progressively thinner interlaced fibers. Rheological testing showed that all hydrogels had viscoelastic behavior and the Young's modulus ranged from 2-6KPa. C2C12 myobalsts showed a significant increase in VEGF production and decrease in IL-6 production on LM-111 enriched fibrin hydrogels as compared to pure fibrin hydrogels on day 4...
July 3, 2017: Biomaterials
https://www.readbyqxmd.com/read/28695966/dyrk2-displays-muscle-fiber-type-specific-function-during-zebrafish-early-somitogenesis
#6
Wei Sun, Shuang Jiao, Xungang Tan, Peijun Zhang, Feng You
Dual specificity tyrosine-phosphorylation regulated kinase 2 (DYRK2) is a serine/threonine kinase. In zebrafish, DYRK2 is expressed in the lateral somites and adaxial cells at the early stage of embryo development. However, its role in early myogenesis had not been elucidated yet. Here, we report that DYRK2 mRNA and MyoD mRNA were colocalized in the muscle progenitor cells in somites, including both the posterior compartment of the lateral somites and adaxial cells. Knockdown of DYRK2 reduced the levels of MyoD transcripts in the muscle progenitor cells in somites...
2017: International Journal of Developmental Biology
https://www.readbyqxmd.com/read/28687961/allogeneic-adipose-derived-mesenchymal-stem-cell-transplantation-enhances-the-expression-of-angiogenic-factors-in-a-mouse-acute-hindlimb-ischemic-model
#7
Ngoc Bich Vu, Ha Thi-Ngan Le, Thuy Thi-Thanh Dao, Lan Thi Phi, Ngoc Kim Phan, Van Thanh Ta
Cell migration and molecular mechanisms during healing of damaged vascular or muscle tissues are emerging fields of interest worldwide. The study herein focuses on evaluating the role of allogenic adipose-derived mesenchymal stem cells (ADMSCs) in restoring damaged tissues. Using a hindlimb ischemic mouse model, ADMSC-mediated induction of cell migration and gene expression related to myocyte regeneration and angiogenesis were evaluated. ADMSCs were labeled with GFP (ADMSC-GFP). The proximal end of the femoral blood vessel of mice (over 6 months of age) are ligated at two positions then cut between the two ties...
July 8, 2017: Advances in Experimental Medicine and Biology
https://www.readbyqxmd.com/read/28669802/the-rna-surveillance-factor-upf1-represses-myogenesis-via-its-e3%C3%A2-ubiquitin-ligase-activity
#8
Qing Feng, Sujatha Jagannathan, Robert K Bradley
UPF1 is an RNA helicase that orchestrates nonsense-mediated decay and other RNA surveillance pathways. While UPF1 is best known for its basal cytoprotective role in degrading aberrant RNAs, UPF1 also degrades specific, normally occurring mRNAs to regulate diverse cellular processes. Here we describe a role for UPF1 in regulated protein decay, wherein UPF1 acts as an E3 ubiquitin ligase to repress human skeletal muscle differentiation. Suppressing UPF1 accelerates myogenesis, while ectopically increasing UPF1 levels slows myogenesis...
June 16, 2017: Molecular Cell
https://www.readbyqxmd.com/read/28667428/the-secretory-leukocyte-protease-inhibitor-mrna-expression-is-involved-with-inflammatory-control-after-downhill-exercise-in-the-triceps-brachii-intermediary-head-in-wistar-rats
#9
André Luis Araujo Minari, Lila Missae Oyama, Ronaldo Vagner Thomatieli Dos Santos
After severe skeletal muscle damage, communication between inflammatory macrophages, myogenic cells, and modulatory secretion factors is essential to induce re-establishment of skeletal muscle structure. To analyze when characteristic gene expression of macrophages, myogenic cells, and SLPI are modulated after an exercise-induced muscle damage (EIMD) downhill protocol. Twenty-six rats were exposed to an experimental protocol of exercise and euthanized before (CTRL), immediately after (G0), and 24 (G24) and 48 (G48) hours after the exercise...
June 30, 2017: Journal of Muscle Research and Cell Motility
https://www.readbyqxmd.com/read/28664467/phylogenetic-analysis-of-puumala-virus-strains-from-central-europe-highlights-the-need-for-a-full-genome-perspective-on-hantavirus-evolution
#10
Róbert Szabó, Lukáš Radosa, Martina Ličková, Monika Sláviková, Marta Heroldová, Michal Stanko, Milan Pejčoch, Anja Osterberg, Lies Laenen, Susanne Schex, Rainer G Ulrich, Sandra Essbauer, Piet Maes, Boris Klempa
Puumala virus (PUUV), carried by bank voles (Myodes glareolus), is the medically most important hantavirus in Central and Western Europe. In this study, a total of 523 bank voles (408 from Germany, 72 from Slovakia, and 43 from Czech Republic) collected between the years 2007-2012 were analyzed for the presence of hantavirus RNA. Partial PUUV genome segment sequences were obtained from 51 voles. Phylogenetic analyses of all three genome segments showed that the newfound strains cluster with other Central and Western European PUUV strains...
June 29, 2017: Virus Genes
https://www.readbyqxmd.com/read/28654079/in-situ-immunofluorescent-staining-of-autophagy-in-muscle-stem-cells
#11
Francesco Castagnetti, Elisabetta Fiacco, Carol Imbriano, Lucia Latella
Increasing evidence points to autophagy as a crucial regulatory process to preserve tissue homeostasis. It is known that autophagy is involved in skeletal muscle development and regeneration, and the autophagic process has been described in several muscular pathologies and age-related muscle disorders. A recently described block of the autophagic process that correlates with the functional exhaustion of satellite cells during muscle repair supports the notion that active autophagy is coupled with productive muscle regeneration...
June 12, 2017: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/28653589/parasite-dynamics-in-an-invaded-ecosystem-helminth-communities-of-native-wood-mice-are-impacted-by-the-invasive-bank-vole
#12
Karen C Loxton, Colin Lawton, Peter Stafford, Celia V Holland
It is becoming increasingly evident that biological invasions result in altered disease dynamics in invaded ecosystems, with knock-on effects for native host communities. We investigated disease dynamics in an invaded ecosystem, using the helminth communities of the native wood mouse (Apodemus sylvaticus) in the presence and absence of the invasive bank vole (Myodes glareolus) in Ireland. Native wood mice were collected over 2 years from four sites to assess the impact of the presence of the bank vole on wood mouse helminth community dynamics both at the component and infracommunity level...
June 27, 2017: Parasitology
https://www.readbyqxmd.com/read/28649743/l-arginine-modulates-inflammation-and-muscle-regulatory-genes-after-a-single-session-of-resistance-exercise-in-rats
#13
Samuel R L Morais, Victor Gustavo B Brito, Wagner G Mello, Sandra H P Oliveira
We investigated the skeletal muscle adaptation to L-arginine supplementation prior to a single session of resistance exercise (RE) during the early phase of muscle repair. Wistar rats were randomly assigned into non-exercised (Control), RE plus vehicle (RE); RE plus L-arginine (RE+L-arg) and RE plus aminoguanidine (RE+AG) groups. Animals received four doses of either vehicle (0.9%NaCl), L-arg (1g/b.w.), or AG (iNOS inhibitor) (50mg/b.w.). The animals performed a single RE session until the concentric failure (ladder climbing; 80% overload) and the skeletal muscles were harvested at 0, 8, 24 and 48h post RE...
June 26, 2017: Scandinavian Journal of Medicine & Science in Sports
https://www.readbyqxmd.com/read/28638997/follistatin-treatment-suppresses-serca1b-levels-independently-of-other-players-of-calcium-homeostasis-in-c2c12-myotubes
#14
János Fodor, Adrienn Gomba-Tóth, Tamás Oláh, János Almássy, Ernő Zádor, László Csernoch
Follistatin (FS) is a high affinity activin-binding protein, neutralizing the effects of the Transforming Growth Factor-beta (TGF-β) superfamily members, as myostatin (MSTN). Since MSTN emerged as a negative regulator, FS has been considered as a stimulator of skeletal muscle growth and differentiation. Here, we studied the effect of FS administration on the Ca(2+)-homeostasis of differentiating C2C12 skeletal muscle cells. FS-treatment increased the fusion index, the size of terminally differentiated myotubes, and transiently elevated the expression of the calcium-dependent protein phosphatase, calcineurin, at the beginning of differentiation...
June 21, 2017: Journal of Muscle Research and Cell Motility
https://www.readbyqxmd.com/read/28638346/micrornas-in-muscle-characterizing-the-powerlifter-phenotype
#15
Randall F D'Souza, Thomas Bjørnsen, Nina Zeng, Kirsten M M Aasen, Truls Raastad, David Cameron-Smith, Cameron J Mitchell
Powerlifters are the epitome of muscular adaptation and are able to generate extreme forces. The molecular mechanisms underpinning the significant capacity for force generation and hypertrophy are not fully elucidated. MicroRNAs (miRs) are short non-coding RNA sequences that control gene expression via promotion of transcript breakdown and/or translational inhibition. Differences in basal miR expression may partially account for phenotypic differences in muscle mass and function between powerlifters and untrained age-matched controls...
2017: Frontiers in Physiology
https://www.readbyqxmd.com/read/28637957/gm7325-is-myod-dependently-expressed-in-activated-muscle-satellite-cells
#16
Daisuke Takei, Miyuki Nishi, So-Ichiro Fukada, Masao Doi, Hitoshi Okamura, Akiyoshi Uezumi, Lidan Zhang, Morikatsu Yoshida, Mikiya Miyazato, Atsuhiko Ichimura, Hiroshi Takeshima
The Gm7325 gene, bioinformatically identified in the mouse genome, encodes a small protein but has not been characterized until recently. Our gene expression analysis revealed that Gm7325 transcription is remarkably upregulated in injured skeletal muscle tissues. Activated satellite cells and immature myotubes were densely decorated with positive signals for Gm7325 mRNA in in situ hybridization analysis, while no obvious signals were observed in quiescent satellite cells and mature myofibers. In the 5'-flanking regions of mouse Gm7325 and its human homologue, conserved E-box motifs for helix-loop-helix transcription factors are repeatedly arranged around the putative promoter regions...
2017: Biomedical Research
https://www.readbyqxmd.com/read/28621431/adhesion-molecule-kirrel3-neph2-is-required-for-the-elongated-shape-of-myocytes-during-skeletal-muscle-differentiation
#17
Yael Tamir-Livne, Raeda Mubariki, Eyal Bengal
Kirrel/Neph proteins are evolutionarily conserved members of the immunoglobulin superfamily of adhesion proteins. Kirrel3 is the mouse orthologue of Dumbfounded (Duf), a family member that regulates myoblast pre-fusion events in Drosophila. Yet, the role of Kirrel3 in mammalian myogenesis has not been demonstrated. Experiments performed here indicate that the mouse Kirrel3 protein regulates morphological changes of myoblasts that are required for their subsequent fusion into multinucleated myotubes. We show that Kirrel3 is transiently expressed at the tips of myocytes during early myoblast differentiation and that its expression is dependent on the myogenic transcription factor, MyoD...
2017: International Journal of Developmental Biology
https://www.readbyqxmd.com/read/28621161/dna-methylation-patterns-in-ulcerative-colitis-associated-cancer-a-systematic-review
#18
Ruth A Emmett, Katherine L Davidson, Nicholas J Gould, Ramesh P Arasaradnam
BACKGROUND: Evidence points to the role of DNA methylation in ulcerative colitis (UC) associated cancer (UCC), the most serious complication of ulcerative colitis. A better understanding of the etiology of UCC may facilitate the development of new therapeutic targets and help to identify biomarkers of the disease risk. METHODS: A search was performed in three databases following PRISMA protocol. DNA methylation in UCC was compared with sporadic colorectal cancer (SCRC), and individual genes differently methylated in UCC identified...
June 16, 2017: Epigenomics
https://www.readbyqxmd.com/read/28615691/human-myogenic-reserve-cells-are-quiescent-stem-cells-that-contribute-to-muscle-regeneration-after-intramuscular-transplantation-in-immunodeficient-mice
#19
Thomas Laumonier, Flavien Bermont, Pierre Hoffmeyer, Vincent Kindler, Jacques Menetrey
Satellite cells, localized within muscles in vivo, are Pax7(+) muscle stem cells supporting skeletal muscle growth and regeneration. Unfortunately, their amplification in vitro, required for their therapeutic use, is associated with reduced regenerative potential. In the present study, we investigated if human myogenic reserve cells (MRC) obtained in vitro, represented a reliable cell source for muscle repair. For this purpose, primary human myoblasts were freshly isolated and expanded. After 2 days of differentiation, 62 ± 2...
June 14, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28615038/prevalence-and-genetic-variability-of-anaplasma-phagocytophilum-in-wild-rodents-from-the-italian-alps
#20
Fausta Rosso, Valentina Tagliapietra, Ivana Baráková, Marketa Derdáková, Adam Konečný, Heidi Christine Hauffe, Annapaola Rizzoli
BACKGROUND: Human granulocytic anaplasmosis is a zoonotic bacterial disease with increasing relevance for public health in Europe. The understanding of its sylvatic cycle and identification of competent reservoir hosts are essential for improving disease risk models and planning preventative measures. RESULTS: In 2012 we collected single ear biopsy punches from 964 live-trapped rodents in the Province of Trento, Italy. Genetic screening for Anaplasma phagocytophilum (AP) was carried out by PCR amplification of a fragment of the 16S rRNA gene...
June 14, 2017: Parasites & Vectors
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