keyword
MENU ▼
Read by QxMD icon Read
search

Pax3

keyword
https://www.readbyqxmd.com/read/27906583/isolation-of-bovine-skin-derived-precursor-cells-and-their-developmental-potential-after-nuclear-transfer
#1
Jiajia Xiao, Qiaoqiao Li, Pengxiang Qu, Zihan Zhang, Shaohui Pan, Yongsheng Wang, Yong Zhang
Nuclei from less differentiated stem cells yield high cloning efficiency. However, pluripotent stem cells are rather difficult to obtain from bovines. Skin-derived precursor (SKPs) cells exhibit a certain degree of pluripotency, which has been shown to enhance the efficiency of nuclear transfer (NT) in pigs. In this study, bovine SKPs were isolated and characterized. Results showed that bovine SKPs expressed nestin, fibronectin, vimentin, pluripotency-related genes, and characteristic neural crest markers, such as NGFR, PAX3, SOX9, SNAI2, and OCT4...
November 2016: Cellular Reprogramming
https://www.readbyqxmd.com/read/27906130/braf-activates-pax3-to-control-muscle-precursor-cell-migration-during-forelimb-muscle-development
#2
Jaeyoung Shin, Shuichi Watanabe, Soraya Hoelper, Marcus Krüger, Sawa Kostin, Jochen Pöling, Thomas Kubin, Thomas Braun
Migration of skeletal muscle precursor cells is a key step during limb muscle development and depends on the activity of PAX3 and MET. Here, we demonstrate that BRAF serves a crucial function in formation of limb skeletal muscles during mouse embryogenesis downstream of MET and acts as a potent inducer of myoblast cell migration. We found that a fraction of BRAF accumulates in the nucleus after activation and endosomal transport to a perinuclear position. Mass spectrometry based screening for potential interaction partners revealed that BRAF interacts and phosphorylates PAX3...
December 1, 2016: ELife
https://www.readbyqxmd.com/read/27864937/pax3-overexpression-induces-cell-aggregation-and-perturbs-commissural-axon-projection-during-embryonic-spinal-cord-development
#3
Juntang Lin, Sulei Fu, Ciqing Yang, Christoph Redies
Pax3 is a transcription factor that belongs to the paired box family. In the developing spinal cord, it is expressed in the dorsal commissural neurons, which project ascending axons contralaterally to form proper spinal cord-brain circuitry. While it has been shown that Pax3 induces cell aggregation in vitro, little is known about the role of Pax3 in cell aggregation and spinal circuit formation in vivo. We have reported that Pax3 is involved in neuron differentiation and that its overexpression induces ectopic cadherin-7 expression...
November 16, 2016: Journal of Comparative Neurology
https://www.readbyqxmd.com/read/27864345/pax3-foxo1a-expression-in-rhabdomyosarcoma-is-driven-by-the-targetable-nuclear-receptor-nr4a1
#4
Alexandra Lacey, Aline Rodrigues Hoffman, Stephen Safe
Alveolar rhabdomyosarcoma (ARMS) is a devastating pediatric disease driven by expression of the oncogenic fusion gene PAX3-FOXO1A. In this study, we report overexpression of the nuclear receptor NR4A1 is rhabdomyosarcomas which is sufficient to drive high expression of PAX3-FOXO1A there. RNAi-mediated silencing of NR4A1 decreased expression of PAX3-FOXO1A and its downstream effector genes. Similarly, cell treatment with the NR4A1 small molecule antagonists 1,1-bis(3-indolyl)-1-(p-hydroxy or p-carbomethoxyphenyl)methane (C-DIM) decreased PAX3-FOXO1A...
November 18, 2016: Cancer Research
https://www.readbyqxmd.com/read/27859625/loss-of-microrna-489-3p-promotes-osteosarcoma-metastasis-by-activating-pax3-met-pathway
#5
Qifei Liu, Guochun Yang, Yuying Qian
(OS) remains one deadly disease for many affected patients. MicroRNAs (miRNAs) are thought to have an important role in tumor metastasis by regulating diverse cellular pathways. Here, we describe the function and regulation network of miR-489-3p in osteosarcoma (OS) metastasis. MiR-489-3p expression was downregulated in OS cells especially in high metastatic potential cells and was also significantly decreased in metastatic lesions compared with their corresponding primary tumor samples. Both gain- and loss-of-function studies confirmed that miR-489-3p significantly suppressed OS cell invasion and metastasis both in vitro and in vivo...
November 15, 2016: Molecular Carcinogenesis
https://www.readbyqxmd.com/read/27834030/does-the-grass-snake-natrix-natrix-squamata-serpentes-natricinae-fit-the-amniotes-specific-model-of-myogenesis
#6
Damian Lewandowski, Magda Dubińska-Magiera, Ewelina Posyniak, Weronika Rupik, Małgorzata Daczewska
In the grass snake (Natrix natrix), the newly developed somites form vesicles that are located on both sides of the neural tube. The walls of the vesicles are composed of tightly connected epithelial cells surrounding the cavity (the somitocoel). Also, in the newly formed somites, the Pax3 protein can be observed in the somite wall cells. Subsequently, the somite splits into three compartments: the sclerotome, dermomyotome (with the dorsomedial [DM] and the ventrolateral [VL] lips) and the myotome. At this stage, the Pax3 protein is detected in both the DM and VL lips of the dermomyotome and in the mononucleated cells of the myotome, whereas the Pax7 protein is observed in the medial part of the dermomyotome and in some of the mononucleated cells of the myotome...
November 10, 2016: Protoplasma
https://www.readbyqxmd.com/read/27816457/inhibition-of-fgf-signaling-accelerates-neural-crest-cell-differentiation-of-human-pluripotent-stem-cells
#7
Thiranut Jaroonwitchawan, Pattamon Muangchan, Parinya Noisa
Neural crest (NC) is a transient population, arising during embryonic development and capable of differentiating into various somatic cells. The defects of neural crest development leads to neurocristopathy. Several signaling pathways were revealed their significance in NC cell specification. Fibroblast growth factor (FGF) is recognized as an important signaling during NC development, for instance Xenopus and avian; however, its contributions in human species are remained elusive. Here we used human pluripotent stem cells (hPSCs) to investigate the consequences of FGF inhibition during NC cell differentiation...
December 2, 2016: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/27806318/med12-mutations-in-breast-phyllodes-tumors-evidence-of-temporal-tumoral-heterogeneity-and-identification-of-associated-critical-signaling-pathways
#8
Marick Laé, Sophie Gardrat, Sophie Rondeau, Camille Richardot, Martial Caly, Walid Chemlali, Sophie Vacher, Jérôme Couturier, Odette Mariani, Philippe Terrier, Ivan Bièche
Exome sequencing has recently identified highly recurrent MED12 somatic mutations in fibroadenomas (FAs) and phyllodes tumors (PTs). In the present study, based on a large series, we confirmed the presence of MED12 exon 1 and 2 mutations in 49% (41/83) of PTs, 70% (7/10) of FAs and 9.1% (1/11) of fibromatoses. We show that MED12 mutations are associated with benign behavior of phyllodes tumors, as they are detected less frequently in malignant PTs (27.6%) compared to benign (58.3%) and borderline (63.3%) PTs, respectively (p = 0...
October 31, 2016: Oncotarget
https://www.readbyqxmd.com/read/27799565/fusion-transcriptome-profiling-provides-insights-into-alveolar-rhabdomyosarcoma
#9
Zhongqiu Xie, Mihaela Babiceanu, Shailesh Kumar, Yuemeng Jia, Fujun Qin, Frederic G Barr, Hui Li
Gene fusions and fusion products were thought to be unique features of neoplasia. However, more and more studies have identified fusion RNAs in normal physiology. Through RNA sequencing of 27 human noncancer tissues, a large number of fusion RNAs were found. By analyzing fusion transcriptome, we observed close clusterings between samples of same or similar tissues, supporting the feasibility of using fusion RNA profiling to reveal connections between biological samples. To put the concept into use, we selected alveolar rhabdomyosarcoma (ARMS), a myogenic pediatric cancer whose exact cell of origin is not clear...
November 15, 2016: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/27775225/characterization-of-phenotypic-and-transcriptional-differences-in-human-pluripotent-stem-cells-under-2d-and-3d-culture-conditions
#10
Ken-Ichiro Kamei, Yoshie Koyama, Yumie Tokunaga, Yasumasa Mashimo, Momoko Yoshioka, Christopher Fockenberg, Rowland Mosbergen, Othmar Korn, Christine Wells, Yong Chen
Human pluripotent stem cells hold great promise for applications in drug discovery and regenerative medicine. Microfluidic technology is a promising approach for creating artificial microenvironments; however, although a proper 3D microenvironment is required to achieve robust control of cellular phenotypes, most current microfluidic devices provide only 2D cell culture and do not allow tuning of physical and chemical environmental cues simultaneously. Here, the authors report a 3D cellular microenvironment plate (3D-CEP), which consists of a microfluidic device filled with thermoresponsive poly(N-isopropylacrylamide)-β-poly(ethylene glycol) hydrogel (HG), which enables systematic tuning of both chemical and physical environmental cues as well as in situ cell monitoring...
October 24, 2016: Advanced Healthcare Materials
https://www.readbyqxmd.com/read/27760049/helicase-chd4-is-an-epigenetic-coregulator-of-pax3-foxo1-in-alveolar-rhabdomyosarcoma
#11
Maria Böhm, Marco Wachtel, Joana G Marques, Natalie Streiff, Dominik Laubscher, Paolo Nanni, Kamel Mamchaoui, Raffaella Santoro, Beat W Schäfer
A vast number of cancer genes are transcription factors that drive tumorigenesis as oncogenic fusion proteins. Although the direct targeting of transcription factors remains challenging, therapies aimed at oncogenic fusion proteins are attractive as potential treatments for cancer. There is particular interest in targeting the oncogenic PAX3-FOXO1 fusion transcription factor, which induces alveolar rhabdomyosarcoma (aRMS), an aggressive cancer of skeletal muscle cells for which patient outcomes remain dismal...
November 1, 2016: Journal of Clinical Investigation
https://www.readbyqxmd.com/read/27759048/molecular-etiology-and-genotype-phenotype-correlation-of-chinese-han-deaf-patients-with-type-i-and-type-ii-waardenburg-syndrome
#12
Lianhua Sun, Xiaohua Li, Jun Shi, Xiuhong Pang, Yechen Hu, Xiaowen Wang, Hao Wu, Tao Yang
Waardenburg syndrome (WS) characterized by sensorineural hearing loss and pigmentary abnormalities is genetically heterogeneous and phenotypically variable. This study investigated the molecular etiology and genotype-phenotype correlation of WS in 36 Chinese Han deaf probands and 16 additional family members that were clinically diagnosed with WS type I (WS1, n = 8) and type II (WS2, n = 42). Mutation screening of six WS-associated genes detected PAX3 mutations in 6 (86%) of the 7 WS1 probands. Among the 29 WS2 probands, 13 (45%) and 10 (34%) were identified with SOX10 and MITF mutations, respectively...
October 19, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27751815/comparative-analysis-of-the-neurula-transcriptomes-of-two-species-of-flatfishes-platichthys-stellatus-and-paralichthys-olivaceus
#13
Fen Wei, Jie Chen, Xinye Chen, Baolong Bao
Flatfish with left-right eye asymmetry are the most significant among vertebrates. However, the genetic basis for the control of this characteristic is still unclear. We propose that the gene(s) for eye asymmetry initially control minor differences in cell number in the tissues around the eyes during development. This minor difference is then amplified, causing eye migration during metamorphosis. Therefore, comparing the neurula transcriptomes between flatfish species with different eye-reversal mutants may provide very useful information to screen for genes involved in eye asymmetry...
October 14, 2016: Gene
https://www.readbyqxmd.com/read/27730449/integrated-transcriptome-analysis-of-human-ips-cells-derived-from-a-fragile-x-syndrome-patient-during-neuronal-differentiation
#14
Ping Lu, Xiaolong Chen, Yun Feng, Qiao Zeng, Cizhong Jiang, Xianmin Zhu, Guoping Fan, Zhigang Xue
Fragile X syndrome (FXS) patients carry the expansion of over 200 CGG repeats at the promoter of fragile X mental retardation 1 (FMR1), leading to decreased or absent expression of its encoded fragile X mental retardation protein (FMRP). However, the global transcriptional alteration by FMRP deficiency has not been well characterized at single nucleotide resolution, i.e., RNA-seq. Here, we performed in-vitro neuronal differentiation of human induced pluripotent stem (iPS) cells that were derived from fibroblasts of a FXS patient (FXS-iPSC)...
October 11, 2016: Science China. Life Sciences
https://www.readbyqxmd.com/read/27677482/characterization-of-pax3a-and-pax3b-genes-in-artificially-induced-polyploid-and-gynogenetic-olive-flounder-paralichthys-olivaceus-during-embryogenesis
#15
Shuang Jiao, Zhihao Wu, Xungang Tan, Yulei Sui, Lijuan Wang, Feng You
Although chromosome set manipulation techniques including polyploidy induction and gynogentic induction in flatfish are becoming increasingly mature, there exists a poor understanding of their effects on embryonic development. PAX3 plays crucial roles during embryonic myogenesis and neurogenesis. In olive flounder (Paralichthys olivaceus), there are two duplicated pax3 genes (pax3a, pax3b), and both of them are expressed in the brain and muscle regions with some subtle regional differences. We utilized pax3a and pax3b as indicators to preliminarily investigate whether chromosome set manipulation affects embryonic neurogenesis and myogenesis using whole-mount in situ hybridization...
September 27, 2016: Fish Physiology and Biochemistry
https://www.readbyqxmd.com/read/27651778/mirna-targeted-signaling-pathway-in-the-early-stage-of-denervated-fast-and-slow-muscle-atrophy
#16
Gang Li, Qing-Shan Li, Wen-Bin Li, Jian Wei, Wen-Kai Chang, Zhi Chen, Hu-Yun Qiao, Ying-Wei Jia, Jiang-Hua Tian, Bing-Sheng Liang
Denervation often results in skeletal muscle atrophy. Different mechanisms seem to be involved in the determination between denervated slow and fast skeletal muscle atrophy. At the epigenetic level, miRNAs are thought to be highly involved in the pathophysiological progress of denervated muscles. We used miRNA microarrays to determine miRNA expression profiles from a typical slow muscle (soleus muscle) and a typical fast muscle (tibialis anterior muscle) at an early denervation stage in a rat model. Results showed that miR-206, miR-195, miR-23a, and miR-30e might be key factors in the transformation process from slow to fast muscle in denervated slow muscles...
August 2016: Neural Regeneration Research
https://www.readbyqxmd.com/read/27651434/differential-effects-of-pax3-on-expression-of-polysialyltransferases-stx-and-pst-in-tgf-%C3%AE-treated-normal-murine-mammary-gland-cells
#17
Dong Guo, Jia Guo, Xiang Li, Feng Guan
Glycosylation of certain proteins at the mammalian cell surface is an essential event in carcinogenesis. Sialylation, one type of glycosylation, can act on multiple cell-behaviors, such as migration, growth, and malignant invasion. Two polysialyltransferases, ST8Sia II (STX) and ST8Sia IV (PST), are responsible for synthesis of polysialic acid on neural cell adhesion molecule. We showed previously that STX and PST are oppositely expressed in normal murine mammary gland cells undergoing transforming growth factor-β-induced epithelial-mesenchymal transition...
September 19, 2016: Experimental Biology and Medicine
https://www.readbyqxmd.com/read/27639969/existence-of-neural-crest-derived-progenitor-cells-in-normal-and-fuchs-endothelial-dystrophy-corneal-endothelium
#18
Kishore Reddy Katikireddy, Thore Schmedt, Marianne O Price, Francis W Price, Ula V Jurkunas
Human corneal endothelial cells are derived from neural crest and because of postmitotic arrest lack competence to repair cell loss from trauma, aging, and degenerative disorders such as Fuchs endothelial corneal dystrophy (FECD). Herein, we identified a rapidly proliferating subpopulation of cells from the corneal endothelium of adult normal and FECD donors that exhibited features of neural crest-derived progenitor (NCDP) cells by showing absence of senescence with passaging, propensity to form spheres, and increased colony forming efficacy compared with the primary cells...
October 2016: American Journal of Pathology
https://www.readbyqxmd.com/read/27594590/identification-and-characterization-of-the-dermal-panniculus-carnosus-muscle-stem-cells
#19
Neia Naldaiz-Gastesi, María Goicoechea, Sonia Alonso-Martín, Ana Aiastui, Macarena López-Mayorga, Paula García-Belda, Jaione Lacalle, Carlos San José, Marcos J Araúzo-Bravo, Lidwine Trouilh, Véronique Anton-Leberre, Diego Herrero, Ander Matheu, Antonio Bernad, José Manuel García-Verdugo, Jaime J Carvajal, Frédéric Relaix, Adolfo Lopez de Munain, Patricia García-Parra, Ander Izeta
The dermal Panniculus carnosus (PC) muscle is important for wound contraction in lower mammals and represents an interesting model of muscle regeneration due to its high cell turnover. The resident satellite cells (the bona fide muscle stem cells) remain poorly characterized. Here we analyzed PC satellite cells with regard to developmental origin and purported function. Lineage tracing shows that they originate in Myf5(+), Pax3/Pax7(+) cell populations. Skin and muscle wounding increased PC myofiber turnover, with the satellite cell progeny being involved in muscle regeneration but with no detectable contribution to the wound-bed myofibroblasts...
September 13, 2016: Stem Cell Reports
https://www.readbyqxmd.com/read/27588498/acquisition-of-an-oncogenic-fusion-protein-serves-as-an-initial-driving-mutation-by-inducing-aneuploidy-and-overriding-proliferative-defects
#20
Jacob M Loupe, Patrick J Miller, Benjamin P Bonner, Elaine C Maggi, Jyothi Vijayaraghavan, Jovanny Zabaleta, Christopher M Taylor, Fern Tsien, Judy S Crabtree, Andrew D Hollenbach
While many solid tumors are defined by the presence of a particular oncogene, the role that this oncogene plays in driving transformation through the acquisition of aneuploidy and overcoming growth arrest are often not known. Further, although aneuploidy is present in many solid tumors, it is not clear whether it is the cause or effect of malignant transformation. The childhood sarcoma, Alveolar Rhabdomyosarcoma (ARMS), is primarily defined by the t(2;13)(q35;q14) translocation, creating the PAX3-FOXO1 fusion protein...
August 30, 2016: Oncotarget
keyword
keyword
15983
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"