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Laura Blanco-Zubiaguirre, Asier Cabezas, Jose Antonio Carrero, Luis Ángel Fernández, Maitane Olivares, Kepa Castro, Juan Manuel Madariaga
This work describes the development of an analytical procedure for the determination of short chain dicarboxylic acids (C2-C10) both in aqueous and solid matrices such as aerosols and mortars. The developed and validated methodology was based on the preconcentration of compounds by means of mixed-mode solid phase extraction and gas chromatography-mass spectrometry, regardless the analyzed samples. In the case of solid samples, a prior extraction of target compounds using ultrasonic probe-assisted solid-liquid extraction was required...
November 1, 2018: Talanta
Eman Alghamdi, Sergey Piletsky, Elena Piletska
A possibility of harvesting the physiologically-active compounds from the vegetable oils using bespoke resin and optimised solid-phase extraction (SPE) purification method is demonstrated. The paper describes the application of SPE protocol, which was originally developed, using sunflower oil as model biomass, for extraction of the valuable compounds from sesame, wheat germ, palm, olive and soybean oils. As a result, the extraction of three free fatty acids (palmitic, oleic and linoleic), α-tocopherol and three phytosterols (campesterol, stigmasterol and β-sitosterol) from six vegetable oils have been demonstrated...
November 1, 2018: Talanta
Judyta Hejniak, Irena Baranowska, Sandra Stencel, Sylwia Bajkacz
A study of the simultaneous separation and determination of selected polyphenols (rutin, narirutin, naringin, hesperidin, neohesperidin, quercetin, naringenin, kaempferol and hesperetin) with reported effects in the treatment of depression and cardiac and neurodegenerative diseases was performed. An RP-ultrahigh-performance liquid chromatography (UHPLC)-ultraviolet method for analyte separation and determination was successfully developed and validated for a ZORBAX Eclipse Plus C18 RRHD analytical column. Separation was carried out in gradient elution mode with acetonitrile and water modified with 0...
July 31, 2018: Journal of Chromatographic Science
Yao Feng, Wen-Juan Zhang, Yuan-Wang Liu, Jian-Ming Xue, Shu-Qing Zhang, Zhao-Jun Li
Antibiotics, widely used in livestock breeding, enter the environment through animal manure because of incomplete absorption in animals, especially the farmland ecosystem. Therefore, antibiotics may be adsorbed by plants and even become hazardous to human health through the food chain. In this study, a simple, sensitive, and reliable method was developed for the simultaneous determination of eleven antibiotics, including four sulfonamides, two tetracyclines, three fluoroquinolones, tylosin, and chloramphenicol in different vegetable samples using SPE-HPLC-MS/MS...
August 6, 2018: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
Xiaohong Chen, Shengdong Pan, Micong Jin
OBJECTIVE: A method was developed for the determination of pentachlorophenol( PCP) in food by ultrafast liquid chromatography-tandem mass spectrometry after solid phase extraction. METHODS: The sample was extracted in 8%triethylamine/acetonitrile( 70/30, V/V) and purified on a MAX-SPE cartridge. The UFLC separation was performed on a Shim-pack XR-ODS Ⅲ column( 150 mm × 2. 0 mm, 2. 2μm) with a linear gradient elution program of acetonitrile and 5 mmol/L ammonium acetate( 0...
May 2018: Wei Sheng Yan Jiu, Journal of Hygiene Research
Jie Yu, Liming Tan, Tomoaki Kawakami, Peng Wang, Li-Min Fu, Zheng-Yu Wang-Otomo, Jian-Ping Zhang
To explore the photoprotection role of multi-compositional carotenoid (Car) in photosynthetic purple bacteria, we investigated, by means of triplet excitation profile (TEP) combined with steady state optical spectroscopies, the core light-harvesting complex-reaction center of a mutant strain of Rhodobacter (Rba.) sphaeroides (m-LH1-RC) at room temperature. TEP spectra revealed that spheroidene and derivative (Spe) preferentially protect bacteriochlorophylls (BChls) of relatively lower site energy by quenching the triplet excitation (3BChl*), however, spirilloxanthin (Spx) does so irrespective to the site energy of BChls...
August 6, 2018: Journal of Physical Chemistry. B
Heying Zhang, Wei Qu, Yanfei Tao, Dongmei Chen, Shuyu Xie, Lingli Huang, Zhenli Liu, Yuanhu Pan, Zonghui Yuan
This paper presents a convenient and sensitive LC-MS/MS method for the simultaneous determination of carbadox and olaquindox residues, including desoxyolaquindox (DOLQ), desoxycarbadox (DCBX), quinoxaline-2-carboxylic acid (QCA), 3-methyl-quinoxaline-2-carboxylic acid (MQCA), and the glycine conjugates of QCA and MQCA (namely, QCA-glycine and MQCA-glycine, resp.) in swine muscle and liver tissues. Tissue samples were extracted with 2% metaphosphoric acid in 20% methanol and cleaned up by solid-phase extraction (SPE) on a mixed-mode anion-exchange column (Oasis MAX)...
2018: Journal of Analytical Methods in Chemistry
Mengsa Chai, Yihui Chen, Rongrong Xuan, Junfeng Ma, Zhenfeng Jin, Tingting Wang, Dan Qiu, Lihua Zhang, Yukui Zhang
A polyethyleneimine (PEI)-modified attapulgite was employed as a new adsorbent for solid-phase extraction (SPE) of chlorophenols (CPs) from environmental water samples. Key factors pivotal to extraction efficiency, such as organic additive, pH, salt, sample loading volume, elution volume, and sample loading flow rate, were investigated. The maximum adsorption capacity of CPs reached 38 mg/g, and the adsorption behavior could be described with the Langmuir isotherm model. The developed SPE procedure was then tested on river water samples...
August 4, 2018: Analytical and Bioanalytical Chemistry
Zoltán Márta, Balázs Bobály, Jenő Fekete, Balázs Magda, Tímea Imre, Pál Tamás Szabó
A simple, accurate and sensitive micro UHPLC-MS/MS method was developed and validated for the simultaneous determination of 10 nonsteroidal anti-inflammatory drugs (NSAIDs) from different environmental matrices. The micro LC ‒ on-line SPE method described in this study allowed to determine the selected drugs at ultra-trace levels without the most commonly used complex off-line SPE sample preparation procedures. The presented method is capable of reaching satisfactory low LOQ values with analysing the sample directly after being diluted with water...
July 18, 2018: Journal of Pharmaceutical and Biomedical Analysis
Hui Zhang, Simon Watts, Martin C Philix, Shane A Snyder, Choon Nam Ong
Facing the tough challenge of precise measurement of ever-increasing numbers of organic contaminants in the environment, there is an urgent need for more reliable and cost-effective methodologies. In this study, we developed and validated a screening method for analysis of over 450 pesticides in precipitation using gas chromatography with tandem mass spectrometry (GC-MS/MS) in multiple reaction monitoring (MRM) mode. Solid phase extraction (SPE) was applied to extract target analytes from precipitation. Using this targeted approach, we managed to detect 123 pesticides with maximum retention time shifts below 0...
July 26, 2018: Chemosphere
Zhi-Xin Yuan, Sharon Majchrzak-Hong, Gregory S Keyes, Michael J Iadarola, Andrew J Mannes, Christopher E Ramsden
Oxylipins are bioactive mediators that play diverse roles in (patho)physiology. We developed a sensitive and selective ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) method for the simultaneous profiling of 57 targeted oxylipins derived from five major n-6 and n-3 polyunsaturated fatty acids (PUFAs) that serve as oxylipin precursors, including linoleic (LA), arachidonic (AA), alpha-linolenic (ALA), eicosapentaenoic (EPA), and docosahexaenoic (DHA) acids. The targeted oxylipin panel provides broad coverage of lipid mediators and pathway markers generated from cyclooxygenases, lipoxygenases, cytochrome P450 epoxygenases/hydroxylases, and non-enzymatic oxidation pathways...
August 3, 2018: Analytical and Bioanalytical Chemistry
Yang Xu, Dina Goykhman, Ming Wang, Tonya Jackson, Iris Xie, Kenneth Willson, Sheila Breidinger, Helengrace Schuck, Jane Harrelson, Eric J Woolf
Advances in technology have led to a shift for peptide quantification from traditional ligand-binding assays to LC-MS/MS-based analysis, which presents challenges, in other assay sensitivity, specificity and ruggedness, in addition to lacking of regulatory guidance, especially for the hybrid assay format. Methodology & results: This report communicates a strategy that has been employed in our laboratories for method development and assay validation, and exemplified in a case study of MK-2640, a glucose-responsive insulin, in multiple matrices...
July 31, 2018: Bioanalysis
Shuo Li, Mengtian Jia, Hongqiao Guo, Xiaohong Hou
In this work, a variety of highly porous metal organic framework/chitosan (MOF/CS) foams (MIL-53(Al)/CS, MIL-53(Fe)/CS, MIL-101(Cr)/CS, MIL-101(Fe)/CS, UiO-66(Zr)/CS, and MIL-100(Fe)/CS) were designed and prepared by an ice-templating process. The introduction of MOFs made these foams achieve excellent inherent characters in terms of strength, stability, and adsorption ability. The MOFs incorporated in the foams retained their unique properties. Additionally, the foams were durable and their adsorption abilities had only a little loss after being recycled several times...
July 30, 2018: Analytical and Bioanalytical Chemistry
Reneta Gevrenova, Krum Bardarov, Sabine Bouguet-Bonnet, Yulian Voynikov, Vessela Balabanova, Dimitrina Zheleva-Dimitrova, Max Henry
Glucuronide Oleanane-type Triterpenoid Carboxylic Acid 3, 28-Bidesmosides (GOTCAB) saponins are bioactive natural compounds spread in Caryophyllidae. The high complexity of GOTCAB occurring as closely related isobaric and positional isomers is a challenge in their separation and identification. A new liquid chromatography - high resolution Orbitrap mass spectrometry acquisition strategy would be important for the structural elucidation of GOTCAB in plant extracts. In this study, the fragmentation behaviors of GOTCAB from methanol-aqueous root extract of Gypsophila glomerata Pall ex M...
July 21, 2018: Journal of Pharmaceutical and Biomedical Analysis
Frederick Verbeke, Nathan Debunne, Yorick Janssens, Liesa Tack, Evelien Wynendaele, Petra Rigole, Tom Coenye, Bart De Spiegeleer
Bacteria communicate with each other using quorum sensing; i.e. the production and sensing of signalling molecules. Enterococcus faecalis, a Gram-positive bacterium, employs peptides as quorum sensing molecules. These peptides have previously been isolated from culture media by elaborate, time and medium-consuming sample preparation approaches and specific bacteria-based bio-sensors. Here, a method for the detection and quantification of all nine currently reported E. faecalis quorum sensing peptides belonging to the RNPP family in bacterial cell culture medium was developed...
July 17, 2018: Journal of Pharmaceutical and Biomedical Analysis
Randall C Robinson, Emeline Colet, Tian Tian, Nina A Poulsen, Daniela Barile
Milk oligosaccharides (OS) are bioactive molecules that impart a variety of health benefits to the consumer. Techniques commonly used to analyse and quantify OS require optimised extraction methods to separate the OS from more abundant milk components. Solid phase extraction (SPE) is frequently used to isolate milk OS from lactose; however, the literature contains no formal studies on its efficacy in this application. In this study, established SPE conditions were modified to improve the technique's effectiveness in purifying OS from lactose...
May 2018: International Dairy Journal
Maryam Shamsayei, Yadollah Yamini, Hamid Asiabi
In this study a novel "yarn-in-tube" configuration was introduced by packing cotton yarn inside stainless steel cartridge named packed yarn-in-tube solid phase extraction (yarn-IT-SPE) followed by high performance liquid chromatography. For the first time, cotton yarns were coated with a new polypyrrole@copper-chromium-iron ternary layered double hydroxide nanocomposite (Yarn@PPy@Cu-Cr-Fe LDH). The yarn@PPy@Cu-Cr-Fe LDH sorbent exhibited flexible substrate, high porosity, a three-dimensional, high sorbent loading, long lifetime, good mechanical stability, high anion-exchange capacity and large specific surface area as a result it is a good choice for the separation and preconcentration of acidic cholorophenols in honey samples...
July 24, 2018: Journal of Chromatography. A
Cunying Huang, Huihui Wan, Jing Zhang, Hongmin Zhong, Juan Li, YuMing Sun, Qing Wang, Hua Zhang
An assay method to quantify ondansetron (OND), granisetron (GRA) and tropisetron (TRO) in goat plasma has been successfully developed and validated. This method procedure for the analysis of OND, GRA and TRO was involved of extracting samples with hydrophilic interaction liquid chromatography (HILIC) solid phase extraction (SPE) and determination by liquid chromatography coupled to tandem mass spectroscopy. An SPE method for the simultaneous extraction of OND, GRA and TRO with high efficiency and selectivity was developed...
July 11, 2018: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
Angelica Bianco, Laurent Deguillaume, Mickaël Vaïtilingom, Edith Nicol, Jean-Luc Baray, Nadine Chaumerliac, Maxime Cyril Bridoux
Cloud droplets contain dynamic and complex pools of highly heterogeneous organic matter, resulting from the dissolution of both water soluble organic carbon in atmospheric aerosol particles and gas-phase soluble species, and are constantly impacted by chemical, photochemical and biological transformations. Cloud samples from two summer events, characterized by different air masses and physicochemical properties, were collected at the puy de Dôme station in France, concentrated on a strata-X solid-phase extraction (SPE) cartridge and directly infused using electrospray ionization in the negative mode (ESI-) coupled with ultrahigh-resolution mass spectrometry (UHRMS)...
July 27, 2018: Environmental Science & Technology
Min Jiang, Xuelian Li, Xiaowei Quan, Xiaoying Li, Baosen Zhou
(1) Background . Non-small cell lung cancer (NSCLC) has a high mortality rate. MiRNAs have been found to be diagnostic biomarkers for NSCLC. However, controversial results exist. We conducted this meta-analysis to evaluate the diagnostic value of miRNAs for NSCLC. (2) Methods . Databases and reference lists were searched. Pooled sensitivity (SEN), specificity (SPE), and area under the curve (AUC) were applied to examine the general diagnostic efficacy, and subgroup analysis was also performed. (3) Results ...
2018: BioMed Research International
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