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https://www.readbyqxmd.com/read/30121041/dual-carbon-chlorine-isotope-fractionation-during-dichloroelimination-of-1-1-2-trichloroethane-by-an-enrichment-culture-containing-dehalogenimonas-sp
#1
Mònica Rosell, Jordi Palau, Siti Hatijah Mortan, Gloria Caminal, Albert Soler, Orfan Shouakar-Stash, Ernest Marco-Urrea
Chlorinated ethanes are frequent groundwater contaminants but compound specific isotope analysis (CSIA) has been scarcely applied to investigate their degradation pathways. In this study, dual carbon and chlorine isotope fractionation was used to investigate for the first time the anoxic biodegradation of 1,1,2-trichloroethane (1,1,2-TCA) using a Dehalogenimonas-containing culture. The isotopic fractionation values obtained for the biodegradation of 1,1,2-TCA were ɛC  = -6.9 ± 0.4‰ and ɛCl  = -2...
August 6, 2018: Science of the Total Environment
https://www.readbyqxmd.com/read/30121016/high-sensitivity-c-reactive-protein-elevation-in-patients-with-prior-myocardial-infarction-in-the-united-states
#2
Neha J Pagidipati, Anne S Hellkamp, Puza P Sharma, Tracy Y Wang, Gregg C Fonarow, Michael Pencina
IMPORTANCE: The extent to which levels of high-sensitivity C-reactive protein (hs-CRP), a known marker of increased cardiovascular risk, are elevated and are associated with standard cardiovascular risk factors in patients with a history of myocardial infarction (MI) is unknown. OBJECTIVES: To determine the pattern and determinants of the distribution of hs-CRP in those with a prior MI in the United States using a nationally representative sample. DESIGN AND PARTICIPANTS: Adults with hs-CRP data in the National Health and Nutrition Examination Surveys from 1999-2010...
July 27, 2018: American Heart Journal
https://www.readbyqxmd.com/read/30120993/synthesis-of-the-cytotoxic-phytosphingosines-and-their-isomeric-analogues
#3
Jana Špaková Raschmanová, Miroslava Martinková, Jozef Gonda, Martina Bago Pilátová, Daniel Kupka, Dávid Jáger
A straightforward synthesis of l-lyxo- and l-xylo-phytosphingosine along with their isomeric analogues has been accomplished. The salient features of this approach are the utilization of [3,3]-sigmatropic rearrangements to install a C-N bond and application of a late stage Wittig or OCM reaction to incorporate the hydrophobic chain unit. The final compounds were evaluated regarding their ability to alter both leukaemia and solid tumor cancer cells viability.
August 6, 2018: Carbohydrate Research
https://www.readbyqxmd.com/read/30120991/sclerostin-and-its-significance-for-children-and-adolescents-with-type-1-diabetes-mellitus-t1d
#4
Anna Wędrychowicz, Krystyna Sztefko, Jerzy B Starzyk
INTRODUCTION: Recent studies have shown that sclerostin, which is a negative regulator of bone formation, could play an important role in the crosstalk between bone and glucose metabolism. The role of sclerostin and its link with glucose homeostasis in type 1 diabetes mellitus (T1D) has not been yet studied extensively in children. The aim of this study was to assess sclerostin and its relationship between other bone and fat related factors as well as glucose metabolism in children and adolescents with T1D in comparison to their healthy peers...
August 15, 2018: Bone
https://www.readbyqxmd.com/read/30120942/targeted-temperature-management-with-total-liquid-ventilation-after-ischemic-spinal-cord-injury
#5
Nicolas Mongardon, Matthias Kohlhauer, Fanny Lidouren, Mariana Barretto, Philippe Micheau, Clovis Adam, Gilles Dhonneur, Bijan Ghaleh, Renaud Tissier
BACKGROUND: Ischemic spinal cord injury is a devastating condition after aortic surgery. We determined whether ultrafast and short whole-body hypothermia provided by total liquid ventilation (TLV) attenuated lower limb paralysis after aortic cross-clamping with a targeted temperature management at 33°C vs 36°C. METHODS: Anesthetized rabbits were submitted to infrarenal aortic cross-clamping during 15 min. A Control group (n=7) was maintained at normothermia (38-38...
August 15, 2018: Annals of Thoracic Surgery
https://www.readbyqxmd.com/read/30120893/early-arrival-at-breeding-grounds-causes-costs-and-a-trade-off-with-overwintering-latitude
#6
Shay Rotics, Michael Kaatz, Sondra Turjeman, Damaris Zurell, Martin Wikelski, Nir Sapir, Ute Eggers, Wolfgang Fiedler, Florian Jeltsch, Ran Nathan
1.Early arrival at breeding grounds is of prime importance for migrating birds as it is known to enhance breeding success. Adults, males and higher quality individuals typically arrive earlier, and across years, early arrival has been linked to warmer spring temperatures. However, the mechanisms and potential costs of early arrival are not well understood. 2.To deepen the understanding of arrival date differences between individuals and years, we studied them in light of the preceding spring migration behaviour and atmospheric conditions en route...
August 18, 2018: Journal of Animal Ecology
https://www.readbyqxmd.com/read/30120829/effect-of-rehabilitation-on-fatigue-level-in-patients-with-multiple-sclerosis
#7
Wioletta Mikuľáková, Eleonóra Klímová, Lucia Kendrová, Miloslav Gajdoš, Marek Chmelík
BACKGROUND The objective of this study was to evaluate the effect of a rehabilitation program in changing the perception of fatigue in patients with multiple sclerosis. MATERIAL AND METHODS The study involved 65 respondents/patients with clinically confirmed multiple sclerosis (54 women, 11 men, average age 46.49 years). The evaluation of the effects of fatigue on the physical, psychological, and psychosocial aspects of life was assessed using the Modified Fatigue Impact Scale (MFIS). To test the effectiveness of the neurorehabilitation program, we enrolled 2 groups: the experimental group (EG, n=32, 29 women, 3 men, Expanded Disability Status Scale (EDSS) 4...
August 18, 2018: Medical Science Monitor: International Medical Journal of Experimental and Clinical Research
https://www.readbyqxmd.com/read/30120824/construction-of-a-recombinant-rhinovirus-accommodating-fluorescent-marker-expression
#8
Mingyuan Han, Charu Rajput, Joanna L Hinde, Qian Wu, Jing Lei, Tomoko Ishikawa, J Kelley Bentley, Marc B Hershenson
BACKGROUND: Rhinovirus (RV) causes the common cold and asthma exacerbations. The RV genome is a 7.3 kb single-strand positive-sense RNA. OBJECTIVE: Using minor group RV1A as a backbone, we sought to design and generate a recombinant RV1A accommodating fluorescent marker expression, thereby allowing tracking of viral infection. METHOD: Recombinant RV1A infectious cDNA clones harboring the coding sequence of green fluorescent protein (GFP), Renilla luciferase or iLOV (for light, oxygen or voltage sensing) were engineered and constructed...
August 17, 2018: Influenza and Other Respiratory Viruses
https://www.readbyqxmd.com/read/30120814/general-and-sex-profile-of-women-with-partner-affected-by-premature-ejaculation-results-of-a-large-observational-non-interventional-cross-sectional-epidemiological-study-iper-f
#9
P Verze, D Arcaniolo, C Imbimbo, T Cai, L Venturino, L Spirito, G Califano, R La Rocca, V Mirone
BACKGROUND: Couple distress is a crucial point in premature ejaculation (PE). PE has been associated with significant bother, interpersonal problems, and dissatisfaction with sexual intercourse for both men and their partners. OBJECTIVES: The primary objective of this study was to assess the effect of PE on female sexuality in female partners of men affected from PE. Secondary objectives were to assess the impact of PE on female sexual quality of life, to assess the presence of sexual problems of the male partner, and to evaluate the prevalence and characteristics of comorbidities...
August 17, 2018: Andrology
https://www.readbyqxmd.com/read/30120807/the-effect-of-dietary-amylose-amylopectin-ratio-on-serum-and-hepatic-lipid-content-and-its-molecular-mechanisms-in-growing-finishing-pigs
#10
C Yang, J He, B Yu, J Yu, X B Mao, D W Chen, Y L Yin
This study was conducted to investigate the effects of dietary amylose/amylopectin ratio (DAR) on serum and hepatic lipid content, luminal short-chain fatty acid (SCFA) concentrations, and the expression of host genes involved in fat and glucose metabolism in liver and mucosa in growing-finishing pigs. Forty-eight Duroc × Landrace × Large White pigs (49.8 ± 2.8 kg) were randomly allocated to low amylose/amylopectin ratio (LR) and high amylose/amylopectin ratio (HR) groups, each group consisting of six replicates (pen) with four pigs per pen...
August 17, 2018: Journal of Animal Physiology and Animal Nutrition
https://www.readbyqxmd.com/read/30120805/electrospray-ionization-tandem-mass-spectrometry-of-labdane-type-acid-diterpenes
#11
Gabriela P Aguiar, Eduardo J Crevelin, Herbert J Dias, Sérgio R Ambrósio, Jairo K Bastos, Vladimir C G Heleno, Ricardo Vessecchi, Antônio E M Crotti
Copaifera (Leguminoseae) species produce a commercially interesting oleoresin that displays several biological activities, including antimicrobial and anti-inflammatory properties. Labdane-type diterpenes are the main chemical constituents of these oleoresins, and copalic acid is the only compound that has been detected in all Copaifera oleoresins. In this study, we investigate some aspects of the gas-phase fragmentation reactions involved in the formation of the product ions from the deprotonated compounds (-)-copalic acid (1), (-)-3β-hydroxy-copalic acid (2), (-)-3β-acetoxy-copalic acid (3), and (-)-ent-agathic acid (4) by electrospray ionization tandem mass spectrometry (ESI-MS/MS) and multiple stage mass spectrometry (MSn )...
August 17, 2018: Journal of Mass Spectrometry: JMS
https://www.readbyqxmd.com/read/30120788/a-novel-enzymatic-method-for-the-measurement-of-lactose-in-lactose-free-products
#12
D Mangan, B V McCleary, H Culleton, C Cornaggia, R Ivory, V McKie, E Delaney, T Kargelis
BACKGROUND: In recent years there has been a surge in the number of commercially available lactose-free variants of a wide variety of products. This presents an analytical challenge for the measurement of the residual lactose content in the presence of high levels of mono-, di-, and oligosaccharides. RESULTS: In the current work, we describe the development of a novel enzymatic low-lactose determination method termed LOLAC which is based on an optimised glucose removal pre-treatment step followed by a sequential enzymatic assay which measures residual glucose and lactose in a single cuvette...
August 17, 2018: Journal of the Science of Food and Agriculture
https://www.readbyqxmd.com/read/30120772/consistent-ldl-c-response-with-evolocumab-among-patient-subgroups-in-proficio-a-pooled-analysis-of-3146-patients-from-phase-3-studies
#13
Erik Stroes, Jennifer G Robinson, Frederick J Raal, Robert Dufour, David Sullivan, Helina Kassahun, Yuhui Ma, Scott M Wasserman, Michael J Koren
BACKGROUND: Evolocumab significantly lowers low-density lipoprotein cholesterol (LDL-C) when dosed 140 mg every 2 weeks (Q2W) or 420 mg monthly (QM) subcutaneously. HYPOTHESIS: LDL-C changes are comparable among different patient subgroups in a pooled analysis of data from phase 3 trials. METHODS: A total of 3146 patients received ≥1 dose of evolocumab or control in four 12-week phase 3 studies. Percent change from baseline in LDL-C for evolocumab 140 mg Q2W or 420 mg QM vs control was reported as the average of week 10 and 12 values...
August 17, 2018: Clinical Cardiology
https://www.readbyqxmd.com/read/30120767/association-of-macrophage-inhibitory-factor-173-gene-polymorphism-with-biological-behavior-of-prostate-cancer
#14
Mohammad Reza Razzaghi, Mohammad Mohsen Mazloomfard, Sheida Malekian, Zahra Razzaghi
PURPOSE: Chronic inflammation is an important factor in the etiology of prostate cancer. Macrophage migration inhibitory factor (MIF) plays an important regulatory role in inflammatory responses. The aim of this study was to investigate the potential association between MIF-173 G/C polymorphism, and both biological behavior and incidence of prostate cancer. MATERIALS AND METHODS: Analysis of polymorphic variants for MIF was performed using the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method in 128 subjects with prostate cancer and 135 controls...
August 14, 2018: Urology Journal
https://www.readbyqxmd.com/read/30120736/a-community-partnered-approach-to-developing-church-based-interventions-to-reduce-health-disparities-among-african-americans-and-latinos
#15
Kathryn P Derose, Malcolm V Williams, Cheryl A Branch, Karen R Flórez, Jennifer Hawes-Dawson, Michael A Mata, Clyde W Oden, Eunice C Wong
Faith and public health partnerships offer promise to addressing health disparities, but examples that incorporate African-Americans and Latino congregations are lacking. Here we present results from developing a multi-ethnic, multi-denominational faith and public health partnership to address health disparities through community-based participatory research (CBPR), focusing on several key issues: (1) the multi-layered governance structure and activities to establish the partnership and identify initial health priority (obesity), (2) characteristics of the congregations recruited to partnership (n = 66), and (3) the lessons learned from participating congregations' past work on obesity that informed the development of a multi-level, multi-component, church-based intervention...
August 17, 2018: Journal of Racial and Ethnic Health Disparities
https://www.readbyqxmd.com/read/30120728/adsorption-characteristics-of-methylene-blue-by-biochar-prepared-using-sheep-rabbit-and-pig-manure
#16
Wen Huang, Jiao Chen, Jianqiang Zhang
Sheep manure biochar (SMB500 ), rabbit faeces biochar (RFB500 ) and pig manure biochar (PMB500 ) prepared by controlled thermal decomposition at 500 °C were used to adsorb methylene blue (MB) in water. Elemental analysis, BET and SEM results showed that the specific surface area, total pore volume and average pore diameter of SMB500 were 7.59, 4.20 and 1.16 times greater than those of RFB500 , which were also 12.02, 6.88 and 1.37 times greater than those of PMB500, respectively. SMB500 had stronger stability and aromaticity...
August 17, 2018: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/30120707/spirulina-supplementation-during-the-transition-period-by-grazing-dairy-cattle-at-tropical-highland-conditions
#17
C N Garcés, D Vela, A Mullo, V Cabezas, A Alvear, C H Ponce
The objective of this experiment was to evaluate the effects of spirulina supplementation on oxidative stress, immunity, and productive performance during the transition period by grazing dairy cattle. Thirty multiparous gestating cows with an initial body weight (BW = 544 ± 57 kg) were enrolled in this experiment and were stratified by expected calving date. Cows were randomly assigned to one of the three experimental groups: (1) control, no supplementation of spirulina; (2) spirulina-15 (15 g/day of spirulina); and (3) spirulina-30 (30 g/day of spirulina)...
August 17, 2018: Tropical Animal Health and Production
https://www.readbyqxmd.com/read/30120700/comparison-of-central-laboratory-assessments-of-er-pr-her2-and-ki67-by-ihc-fish-and-the-corresponding-mrnas-esr1-pgr-erbb2-and-mki67-by-rt-qpcr-on-an-automated-broadly-deployed-diagnostic-platform
#18
Natalie C Wu, Wendy Wong, Kenneth E Ho, Victor C Chu, Annaliza Rizo, Simon Davenport, Devon Kelly, Rosemary Makar, Jacek Jassem, Renata Duchnowska, Wojciech Biernat, Barbara Radecka, Tomoyuki Fujita, Jonathan L Klein, Mark Stonecypher, Shoichiro Ohta, Hartmut Juhl, Jodi M Weidler, Michael Bates, Michael F Press
PURPOSE: The methods (IHC/FISH) typically used to assess ER, PR, HER2, and Ki67 in FFPE specimens from breast cancer patients are difficult to set up, perform, and standardize for use in low and middle-income countries. Use of an automated diagnostic platform (GeneXpert®) and assay (Xpert® Breast Cancer STRAT4) that employs RT-qPCR to quantitate ESR1, PGR, ERBB2, and MKi67 mRNAs from formalin-fixed, paraffin-embedded (FFPE) tissues facilitates analyses in less than 3 h. This study compares breast cancer biomarker analyses using an RT-qPCR-based platform with analyses using standard IHC and FISH for assessment of the same biomarkers...
August 17, 2018: Breast Cancer Research and Treatment
https://www.readbyqxmd.com/read/30120698/diagnostic-performance-of-11-c-choline-pet-ct-and-fdg-pet-ct-for-staging-and-restaging-of-renal-cell-cancer
#19
Yukako Nakanishi, Kazuhiro Kitajima, Yusuke Yamada, Takahiko Hashimoto, Toru Suzuki, Shuken Go, Akihiro Kanematsu, Michio Nojima, Koichiro Yamakado, Shingo Yamamoto
PURPOSE: To compare findings obtained with 11 C-choline and FDG PET/CT scanning for renal cell carcinoma staging and restaging. MATERIALS AND METHODS: Twenty-eight renal cell carcinoma patients whose histological subtype was clear cell type in 26 and papillary type in 2, while Fuhrman nuclear grade was G1,2 in 16 and G3,4 in 12, underwent both 11 C-choline and FDG PET/CT examinations before (n = 10) and/or after (n = 18) treatment, then those scanning modalities were compared in regard to patient- and lesion-based diagnostic performance using 5 grading scores...
August 17, 2018: Annals of Nuclear Medicine
https://www.readbyqxmd.com/read/30120683/group-communication-analysis-a-computational-linguistics-approach-for-detecting-sociocognitive-roles-in-multiparty-interactions
#20
Nia M M Dowell, Tristan M Nixon, Arthur C Graesser
Roles are one of the most important concepts in understanding human sociocognitive behavior. During group interactions, members take on different roles within the discussion. Roles have distinct patterns of behavioral engagement (i.e., active or passive, leading or following), contribution characteristics (i.e., providing new information or echoing given material), and social orientation (i.e., individual or group). Different combinations of roles can produce characteristically different group outcomes, and thus can be either less or more productive with regard to collective goals...
August 17, 2018: Behavior Research Methods
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