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Abu S M Mohsin, Mariam B Salim
Background: We have introduced a novel method to quantify the intracellular refractive index (RI) of living cells and determine the molecular interaction of two interacting molecules using single particle spectroscopy. The advantages of this proposed technique over fluorescence-based imaging techniques is that it does not require any contrasting agent and it does not blink and bleach. Instead, our technique provides a non-destructive, non-invasive, high-resolution imaging of live cells...
2018: International Journal of Nanomedicine
Kahina Mehennaoui, Sylvain Legay, Tommaso Serchi, François Guérold, Laure Giamberini, Arno C Gutleb, Sébastien Cambier
Gene expression profiling via RT-qPCR is a robust technique increasingly used in ecotoxicology. Determination and validation of optimal reference genes is a requirement for initiating RT-qPCR experiments. To our best knowledge, this study is the first attempt of identifying a set of reference genes for the freshwater crustacean Gammarus fossarum. Six candidate genes (Actin, TUB, UB, SDH, Clathrin and GAPDH) were tested in order to determine the most stable ones in different stress conditions and to increase the robustness of RT-qPCR data...
October 15, 2018: Scientific Reports
Mahbobeh Javidi, Mohammad Reza Housaindokht, Asma Verdian, Bibi Marzieh Razavizadeh
In this paper, a novel apta-sensing colorimetric platform for rapid detection of chloramphenicol (CAP) in raw milk was developed. The AuNPs are stabilized by short-sequences aptamers against salt induced aggregation and this is the base of most colorimetric aptasensors development. However, the statute shows low sensitivity for the long-sequence aptamers. Herein, we propose an alternative strategy that use intact long-sequence aptamers for develop a highly sensitive AuNP-based colorimetric aptasensor. Determination of CAP in animal derived foods is an urgent demanded in the effort to minimize food safety risk...
December 18, 2018: Analytica Chimica Acta
Safiya Allie, Ignacio Hegoburu, Min Jeong Shin, Jung Young Jung, Violeta Toader, Alejandro Rey, Ezequiel R Soule, Linda Reven
Liquid crystal (LC)-polymer blends are important stimuli responsive materials already employed in a wide range of applications whereas nanoparticle (NP)-LC blends are an emerging class of nanocomposites. Polymer ligands offer the advantages of synthetic simplicity along with chemical and molecular weight tunability. Here we compare the phase behavior of 5CB blended with poly(ethylene oxide) (PEO) and with gold NPs functionalized with thiolated PEO (AuNP-PEO) as a function of PEO concentration by DSC, POM and 13C NMR spectroscopy...
October 15, 2018: Soft Matter
A Barreto, L G Luis, E Pinto, A Almeida, P Paíga, L H M L M Santos, C Delerue-Matos, T Trindade, A M V M Soares, K Hylland, S Loureiro, M Oliveira
Gold nanoparticles (AuNPs) are found in a wide range of applications and therefore expected to present increasing levels in the environment. There is however limited knowledge concerning the potential toxicity of AuNPs as well as their combined effects with other pollutants. Hence, the present study aimed to investigate the effects of AuNPs alone and combined with the pharmaceutical gemfibrozil (GEM) on different biological responses (behaviour, neurotransmission, biotransformation and oxidative stress) in one of the most consumed fish in southern Europe, the seabream Sparus aurata...
October 1, 2018: Chemosphere
Ayemeh Bagheri Hashkavayi, Jahan Bakhsh Raoof
In this paper, we report the application of a reusable electrochemical aptasensor for detection of tryptophan by using [Fe(bpy)3 ](p-CH3 C6 H4 SO2 )2 as an electroactive indicator and based on the target-compelled aptamer displacement. The aptasensor fabricated by self-assembling the thiolated probe on the surface of graphite screen-printed electrode modified with gold nanoparticles/multiwalled carbon nanotubes and chitosan nanocomposite (AuNPs/MWCNTs-Chit/SPE). Afterward, Trp aptamer (Apt) immobilized on the modified electrode surface through hybridization...
October 3, 2018: Journal of Pharmaceutical and Biomedical Analysis
Yingdi Wu, Ying Li, Huixia Han, Caisheng Zhao, Xiaoru Zhang
A sensitive surface-enhanced Raman scattering (SERS) approach has been developed for detection of microRNA (miRNA) based on target-triggered dual signal amplification including strand displancement amplification (SDA) and hybridization chain reaction (HCR). With the assistant of polymerase and nicking endonuclease (NEase), target miRNA combines with the single stranded template DNA to generate a great amount of trigger DNA which can induce HCR. Coupled the dual cycle amplification of SDA and HCR with the dual enhancement of gold nanoparticles (AuNPs), a low detection limit of 0...
October 9, 2018: Analytical Biochemistry
Suresh K Verma, Ealisha Jha, Pritam Kumar Panda, Puja Kumari, Nandini Pramanik, Shalini Kumari, Arun Thirumurugan
Implication of gold nanoparticles in industrial and day-to-day life products at extensive scale has raised concern about their toxicity to environment and human health. Moreover, quest of new technologies for production of biocompatible nanoparticles increased. This study explores the molecular toxicology of AuNP with enlightenment of their green synthesis using medicinal plant extract as reducing and stabilizing agent. Synthesized CAuNP were characterized for their physiochemical properties by standard techniques like FESEM, TEM, DLS, UV-Vis spectroscopy and FTIR...
October 12, 2018: Artificial Cells, Nanomedicine, and Biotechnology
Liping Zhu, Desheng Chen, Xiaohui Lu, Yan Qi, Pan He, Chenghui Liu, Zhengping Li
Proteins lack the duplication mechanism like nucleic acids, so the connection of immunoassays with effective nucleic acid amplification techniques has become a powerful way for the detection of trace protein biomarkers in biological fluids. However, such immunoassays generally suffer from rather stringent DNA sequence design and complicated operations. Herein, we propose a simple but highly sensitive flow cytometric immunoassay (FCI) by employing on-bead terminal deoxynucleotidyl transferase (TdT)-initiated template-free DNA extension as an effective signal amplification pathway (TdT-FCI), and gold nanoparticles (AuNPs) co-functionalized with both the detection antibody and a 3'-OH oligonucleotide (ODN) as the transducer to bridge the immunoassay and subsequent TdT-mediated DNA amplification...
August 28, 2018: Chemical Science
Elham Shahhoseini, Prabhakar Ramachandran, William Roy Patterson, Moshi Geso
Purpose: The purpose of this study was to determine dose enhancement (DE) and the possible clinical benefits associated with the inclusion of gold nanoparticles (AuNPs) in cancer cells irradiated by either an 192 Ir brachytherapy source or a Xoft® Axxent® Electronic (eBx™) Brachytherapy. Patients and methods: Brachytherapy DE caused by AuNPs is investigated using two methods, namely 192 Ir and eBx™ Brachytherapy. The second method, which was recently introduced clinically, operates at ~50 kV, which is also the optimal beam energy for DE...
2018: International Journal of Nanomedicine
Leonie Aengenheister, Dörthe Dietrich, Amin Sadeghpour, Pius Manser, Liliane Diener, Adrian Wichser, Uwe Karst, Peter Wick, Tina Buerki-Thurnherr
BACKGROUND: Gold nanoparticles (AuNPs) are promising candidates to design the next generation NP-based drug formulations specifically treating maternal, fetal or placental complications with reduced side effects. Profound knowledge on AuNP distribution and effects at the human placental barrier in dependence on the particle properties and surface modifications, however, is currently lacking. Moreover, the predictive value of human placental transfer models for NP translocation studies is not yet clearly understood, in particular with regards to differences between static and dynamic exposures...
October 11, 2018: Journal of Nanobiotechnology
Rajan Ramachandran, Chandran Krishnaraj, V K Abhay Kumar, Stacey L Harper, Thangavelu P Kalaichelvan, Soon-Il Yun
In this study, toxicity of biologically synthesized silver nanoparticles (AgNPs) and gold nanoparticles (AuNPs) was compared using zebrafish as a model organism. At 96 h, LC50 of AgNPs and AuNPs was found to be 24.5 µg/L and 41 mg/L, respectively. Following the LC50 determination, half of the LC50 of AgNPs (12.25 µg/L) and AuNPs (20.5 mg/L) was exposed to adult zebrafishes for 14 days. Morphological changes, liver marker enzymes, reactive oxygen species (ROS) generation, genotoxic effects and mRNA expression levels of oxidative stress and innate immune response related genes were studied using nanoparticle treated gill, liver and blood cells...
October 2018: 3 Biotech
Yuan Liu, Yan Deng, Taotao Li, Zhu Chen, Hui Chen, Song Li, Hongna Liu
A mercury ion aptamer electrochemical biosensor based on a Thymine-Hg2+ -Thymine (T-Hg2+ -T) structure has been constructed and successfully used to detect mercury ions in tap water samples. The aptamer electrochemical biosensor was assembled using a mercury ion aptamer-functionalized AuNPs-modified glass carbon electrode (aptamer/(AuNPs/CS)₂/GCE) capable of specifically detecting mercury ions through a T-Hg2+ -T structure. The experimental results indicated the optimum electrochemical performance of the prepared aptamer biosensor when the (AuNPs/CS)₂/GCE surface was modified with 1...
December 1, 2018: Journal of Biomedical Nanotechnology
Na Li, Mei-Hao Xiang, Jin-Wen Liu, Hao Tang, Jian-Hui Jiang
Spherical nucleic acid (SNA) constructs are promising new single entity materials, which possess significant advantages in biological applications. Current SNA-based drug delivery system typically employed single-layered ss- or ds-DNA as the drug carriers, resulting in limited drug payload capacity and disease treatment. To advance corresponding applications, we developed a novel DNA-programmed polymeric SNA, a long concatamer DNA polymer that is uniformly distributed on gold nanoparticles (AuNPs), by self-assembling from two short alternating DNA building blocks upon initiation of immobilized capture probes on AuNPs, through a supersandwich hybridization reaction...
October 10, 2018: Analytical Chemistry
Yi-Han Wang, Huan Xia, Ke-Jing Huang, Xu Wu, Ying-Ying Ma, Rui Deng, Yun-Fei Lu, Zi-Wei Han
A sensitive aptamer/protein binding-triggered sandwich assay for thrombin is described. It is based on electrochemical-chemical-chemical redox cycling using a glassy carbon electrode (GCE) that was modified with WSe2 and gold nanoparticles (AuNPs). The AuNPs are linked to thrombin aptamer 1 via Au-S bonds. Thrombin is first captured by aptamer 1 and then sandwiched through the simultaneous interaction with AuNPs modified with thrombin-specific aptamer 2 and signalling probe. Subsequently, the DNA-linked AuNP hybrids result in the capture of streptavidin-conjugated alkaline phosphatase onto the modified GCE through the specific affinity reaction for further signal enhancement...
October 9, 2018: Mikrochimica Acta
Antonella Macagnano, Paolo Papa, Joshua Avossa, Viviana Perri, Marcello Marelli, Francesca Sprovieri, Emiliano Zampetti, Fabrizio De Cesare, Andrea Bearzotti, Nicola Pirrone
Passive sampling systems (PASs) are a low cost strategy to quantify Hg levels in air over both different environmental locations and time periods of few hours to weeks/months. For this reason, novel nanostructured materials have been designed and developed. They consist of an adsorbent layer made of titania nanoparticles (TiO₂NPs, ≤25 nm diameter) finely decorated with gold nanoparticles. The TiO₂NPs functionalization occurred for the photocatalytic properties of titania-anatase when UV-irradiated in an aqueous solution containing HAuCl₄...
October 7, 2018: Nanomaterials
Annegret Becker, Miriam Leskau, Barbara L Schlingmann-Molina, Susanne C Hohmeier, Suhayla Alnajjar, Hugo Murua Escobar, Anaclet Ngezahayo
A recombinant produced C-terminus of the C. perfringens enterotoxin (C-CPE) was conjugated to gold nanoparticles (AuNPs) to produce a C-CPE-AuNP complex (C-CPE-AuNP). By binding to claudins, the C- CPE should allow to target the AuNPs onto the claudin expressing tumor cells for a subsequent cell killing by application of the gold nanoparticle-mediated laser perforation (GNOME-LP) technique. Using qPCR and immunocytochemistry, we identified the human Caco-2, MCF-7 and OE-33 as well as the canine TiHoDMglCarc1305 as tumor cells expressing claudin-3, -4 and -7...
October 8, 2018: Scientific Reports
Bing Tian, Jiulong Li, Renjiang Pang, Shang Dai, Tao Li, Yulan Weng, Ye Jin, Yuejin Hua
Understanding the synthetic mechanisms and cell-nanoparticle interactions of biosynthesized and functionalized gold nanoparticles (AuNPs) using natural products is of great importance for developing their applications in nanomedicine. In this study, we detailed the biotransformation mechanism of Au(III) into AuNPs using a hydroxylated tetraterpenoid deinoxanthin (DX) from the extremophile Deinococcus radiodurans. During the process, Au(III) was rapidly reduced to Au(I) and subsequently reduced to Au(0) by deprotonation of the hydroxyl head groups of the tetraterpenoid...
October 8, 2018: ACS Applied Materials & Interfaces
Thi Thanh-Ngan Nguyen, Thanh-Truc Vo, Bich Ngoc-Huong Nguyen, Dinh-Truong Nguyen, Van-Su Dang, Chi-Hien Dang, Thanh-Danh Nguyen
This study presents an efficient and facile method for biosynthesis of silver nanoparticles (AgNPs) and gold nanoparticles (AuNPs) using aqueous extract of burdock root (BR), A. lappa, and their applications. The nanoparticles were characterized by ultraviolet-visible spectrophotometry, X-ray diffraction, transmission electron microscopy, energy dispersive X-ray, thermogravimetry, and differential thermal analysis. AgNPs capped the BR extract (BR-AgNPs) possessed roughly spherical geometry with an average diameter of 21...
October 6, 2018: Environmental Science and Pollution Research International
Xiuzhen Wan, Pengcheng Liu, Xingxing Jin, Xuanxuan Xin, Pengfei Li, Jiang Yuan, Jian Shen
Nitric oxide (NO)-generating materials are beneficial for vascular tissue engineering (VTE) scaffold because the produced NO would enhance endothelial cells viability while inhibit smooth muscle cell (SMC) proliferation and reduce platelet adhesion, resulting in ideal hemocompatibility and endothelialization. Herein, poly(ε-caprolactone) (PCL)/keratin biocomposite mats were first fabricated, followed by in situ gold (Au) nanoparticles loading to afford PCL/keratin/AuNPs mats. These mats were characterized using field emission scanning electron microscopy (FE-SEM), X-ray photoelectron spectroscopy (XPS), X-ray diffraction (XRD), and thermogravimetric analysis (TGA)...
October 5, 2018: Journal of Biomedical Materials Research. Part A
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