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Bremansu Osa-Andrews, Kee W Tan, Angelina Sampson, Surtaj H Iram
Multidrug resistance protein 1 (MRP1) can efflux a wide variety of molecules including toxic chemicals, drugs, and their derivatives out of cells. Substrates of MRP1 include anti-cancer agents, antibiotics, anti-virals, anti-human immunodeficiency virus (HIV), and many other drugs. To identify novel substrates and modulators of MRP1 by exploiting intramolecular fluorescence resonance energy transfer (FRET), we genetically engineered six different two-color MRP1 proteins by changing green fluorescent protein (GFP) insertion sites, while keeping the red fluorescent protein (RFP) at the C-terminal of MRP1...
October 13, 2018: Pharmaceutics
Sunita Kumbhat, Rakhee Gehlot, Kavita Sharma, Urvasini Singh, Vinod Joshi
A surface plasmon resonance (SPR) based immunosensor is presented for highly sensitive and selective detection of 17β-estradiol by the indirect competitive inhibition immuno assay, employing anti-17 β-estradiol antibody as high molecular weight (HMW) interactant. Immobilization of estradiol-BSA conjugate onto the nano thin gold surface was accomplished by covalent amide linkage through self assembled monolayer. The proposed biosensor is simple to fabricate, reproducible and exhibit excellent sensitivity for estrogen (detection limit,1 pg mL-1 ) without any significant interference from structurally similar steroidal hormone, progesterone and non-steroidal compound bisphenol-A...
October 9, 2018: Journal of Pharmaceutical and Biomedical Analysis
Renbing Yang, Wenbo Cheng, Xifeng Chen, Qin Qian, Qiang Zhang, Yujun Pan, Peng Duan, Peng Miao
Urine strips are widely applied for rapid analysis of various indexes of urine for clinical examinations. The tests mainly rely on the application of a urine analyzer, which suffers several drawbacks and cannot meet the requirements of point-of-care testing (POCT). The integration of a smartphone with a biosensor has recently attracted great attention. We herein propose a human vision-based smartphone algorithm for colorimetric analysis of various urine indexes. A CIEDE2000 formula in CIELab color space is applied for the evaluation of color difference, which may greatly improve the analytical performances of urine strips...
September 30, 2018: ACS Omega
Iwona Grabowska, Neha Sharma, Alina Vasilescu, Madalina Iancu, Gabriela Badea, Rabah Boukherroub, Satishchandra Ogale, Sabine Szunerits
Rapid and accurate diagnostic technologies for early-state identification of cardiovascular abnormalities have become of high importance to prevent and attenuate their progression. The capability of biosensors to determine an increase in the concentration of cardiovascular protein biomarkers in circulating blood immediately after a myocardial infarction makes them ideal point-of-care platforms and alternative approaches to electrocardiograms, chest X-rays, and different laboratory-based immunoassays. We report here a generic approach toward multianalyte sensing platforms for cardiac biomarkers by developing aptamer-based electrochemical sensors for brain natriuretic peptide (BNP-32) and cardiac troponin I (cTnI)...
September 30, 2018: ACS Omega
Mansi Gandhi, Desikan Rajagopal, Sampath Parthasarathy, Sudhakaran Raja, Sheng-Tung Huang, Annamalai Senthil Kumar
Most of the common redox mediators such as organic dyes and cyanide ligand-associated metal complex systems that have been used for various electrochemical applications are hazardous nature. Sesamol, a vital nutrient that exists in natural products like sesame seeds and oil, shows several therapeutic benefits including anticancer, antidiabetic, cardiovascular protective properties, etc. Herein, we introduce a new electrochemical redox platform based on a sesamol derivative, sesamol-quinone (Ses-Qn; oxidized sesamol), prepared by the in situ electrochemical oxidation method on a carbon nanoblack chemically modified glassy carbon electrode surface (GCE/CB@Ses-Qn) in pH 7 phosphate buffer solution, for nontoxic and sustainable electrochemical, electroanalytical, and bioelectroanalytical applications...
September 30, 2018: ACS Omega
Annie Lee, Yusuke Hirabayashi, Seok-Kyu Kwon, Tommy L Lewis, Franck Polleux
Mitochondria play numerous critical physiological functions in neurons including ATP production, Ca2+ regulation, lipid synthesis, ROS signaling, and the ability to trigger apoptosis. Recently developed technologies, including in vivo 2-photon imaging in awake behaving mice revealed that unlike in the peripheral nervous system (PNS), mitochondrial transport decreases strikingly along the axons of adult neurons of the central nervous system (CNS). Furthermore, the improvements of genetically-encoded biosensors have enabled precise monitoring of the spatial and temporal impact of mitochondria on Ca2+ , ATP and ROS homeostasis in a compartment-specific manner...
June 2018: Current Opinion in Physiology
Mengmeng Kang, Olayinka Oderinde, Shunli Liu, Qianqian Huang, Wenjing Ma, Fang Yao, Guodong Fu
Since redox-responsive hydrogels have a wide range of applications especially in biochemistry, such as drug release and biosensors, a kind of redox-responsive hydrogel was fabricated based on iron which has two stable oxidation states, while xanthan gum (XG) was selected as a matrix. In this work, characterization of XG-based hydrogel with Fe3+ ions coordination and its reversible sol-gel conversion property were studied. Xanthan gum solutions with different concentrations were coordinated in situ with constant trivalent iron ions concentration to form hydrogels under ambient temperature...
January 1, 2019: Carbohydrate Polymers
Xiaowei Cheng, Le Huang, Xuanyu Yang, Ahmed A Elzatahry, Abdulaziz Alghamdi, Yonghui Deng
Owing to the high costs and instability of natural enzymes, the development of enzyme mimics based on inorganic nanomaterials has attracted a wide concern in recent years. In this work, a stable nanocomposite composed of highly dispersed CeO2 nanoparticles decorated on zeolite Y as support (CeO2 /Y) was synthesized by a facile wet impregnation method, and the CeO2 /Y nanocomposite was firstly proposed as an efficient peroxidase-mimicking nanozyme for accurate detection of H2 O2 and glucose. The best catalyst was the nanocomposite with CeO2 to zeolite Y mass ratio of 0...
September 29, 2018: Journal of Colloid and Interface Science
Ayemeh Bagheri Hashkavayi, Jahan Bakhsh Raoof
In this paper, we report the application of a reusable electrochemical aptasensor for detection of tryptophan by using [Fe(bpy)3 ](p-CH3 C6 H4 SO2 )2 as an electroactive indicator and based on the target-compelled aptamer displacement. The aptasensor fabricated by self-assembling the thiolated probe on the surface of graphite screen-printed electrode modified with gold nanoparticles/multiwalled carbon nanotubes and chitosan nanocomposite (AuNPs/MWCNTs-Chit/SPE). Afterward, Trp aptamer (Apt) immobilized on the modified electrode surface through hybridization...
October 3, 2018: Journal of Pharmaceutical and Biomedical Analysis
Yuan Jia, Hao Sun, Xiao Li, Dongke Sun, Tao Hu, Nan Xiang, Zhonghua Ni
Rapid, disposable, point-of-care (POC) oral fluid testing has gained considerable attention in recent years as saliva contains biomarker and components of the serum proteome that offer important information on both oral and systemic disease. Microfluidic paper-based analytical devices (μPADs) coupled with smartphone reflectance sensing systems have long been considered to be an effective POC tool for the diagnostics of biomarkers in oral fluid. However, the existing portable systems are limited by the poor color distribution in the detection area as well as not being universally applicable...
October 12, 2018: Biomedical Microdevices
Dhakaram Pangeni Sharm, Ramachandran Vijayan, Syed Arif Abdul Rehman, Samudrala Gourinath
The helicase-primase interaction is an essential event in DNA replication and is mediated by the highly variable C-terminal domain of primase (DnaG) and N-terminal domain of helicase (DnaB). To understand the functional conservation despite the low sequence homology of the DnaB-binding domains of DnaGs of eubacteria, we determined the crystal structure of the helicase-binding domain of DnaG from M. tuberculosis ( Mt DnaG-CTD), and did so to a resolution of 1.58 Å. We observed the overall structure of Mt DnaG-CTD to consist of two subdomains, the N-terminal globular region (GR) and the C-terminal helical hairpin region (HHR), connected by a small loop...
October 12, 2018: Biochemical Journal
Waleed M Alshawafi, Musab Aldhahri, Yaaser Q Almulaiky, Numan Salah, Said S Moselhy, Ibrahim H Ibrahim, Reda M El-Shishtawy, Saleh A Mohamed
In the present study, nanodiamond (ND) was blended with polymethyl methacrylate (PMMA) and then electrospun into nanofibers (nfPMMA-ND) for the immobilization of horseradish peroxidase (HRP). The maximum immobilization efficiency of HRP (96%) was detected at 10% ND and pH 7.0. ATR-FTIR, SEM and TEM were used to characterize the immobilized enzyme. The immobilized enzyme retained 60% of its initial activity after ten reuses. The pH was shifted from 7.0 for soluble HRP to 7.5 for the immobilized enzyme. The soluble HRP had an optimum temperature of 30 °C, whereas this temperature was shifted to 40 °C for the immobilized enzyme...
October 12, 2018: Artificial Cells, Nanomedicine, and Biotechnology
Nicole E Snell, Vishnu P Rao, Kendra M Seckinger, Junyi Liang, Jenna Leser, Allison E Mancini, M A Rizzo
Förster resonance energy transfer (FRET) between fluorophores of the same species was recognized in the early to mid-1900s, well before modern heterotransfer applications. Recently, homotransfer FRET principles have re-emerged in biosensors that incorporate genetically encoded fluorescent proteins. Homotransfer offers distinct advantages over the standard heterotransfer FRET method, some of which are related to the use of fluorescence polarization microscopy to quantify FRET between two fluorophores of identical color...
October 11, 2018: Biosensors
Xiaojin Luo, Weihua Shi, Haoming Yu, Zhaoyang Xie, Kunyi Li, Yue Cui
Developing a simple and direct approach for interfacing a sensor and a target analyte is of great interest for fields such as medical diagnosis, threat detection, food quality control, and environmental monitoring. Gloves provide a unique interface for sensing applications. Here, we show for the first time the development of wearable carbon nanotube (CNT)-based amperometric biosensors painted onto gloves as a new sensing platform, used here for the determination of lactate. Three sensor types were studied, configured as: two CNT electrodes; one CNT electrode, and an Ag/AgCl electrode, and two CNT electrodes and an Ag/AgCl electrode...
October 11, 2018: Sensors
Masarra M Sakr, Khaled M Aboshanab, Walid F Elkhatib, Mahmoud A Yassien, Nadia A Hassouna
The increasing occurrence of resistance among Pseudomonas aeruginosa clinical isolates necessitates finding alternatives to antibiotics for controlling the infection of such pathogenic bacteria. In this study, lactonase gene ahl-1 from Bacillus weihenstephanensis isolate-P65 was successfully cloned and expressed in Escherichia coli BL21 (DE3) under the control of T7 promoter for utilizing its quorum quenching activity against three multidrug-resistant (MDR) P. aeruginosa clinical isolates. The biological activity of the overexpressed lactonase enzyme (Ahl-1), tested using a synthetic signal and Chromobacterium violaceum CV026 as a biosensor, displayed good catalytic activity using hexanoyl homoserine lactone (HHL) as a substrate and Chromobacterium violaceum (CV026) as a biosensor (77...
October 11, 2018: Applied Microbiology and Biotechnology
C S Pundir, Seema Jakhar, Vinay Narwal
Urea is the major end product of nitrogen metabolism in humans, which is eliminated from the body mainly by the kidneys through urine but is also secreted in body fluids such as blood and saliva. Its level in urine ranges from 7 to 20 mg/dL, which drastically rises under patho-physiological conditions thus providing key information of renal function and diagnosis of various kidney and liver disorders. Increase in urea levels in blood, also referred to as azotemia or uremia. The chronic kidney disease (CKD) or end stage renal disease (ESRD) is generally caused due to the progressive loss of kidney function...
September 20, 2018: Biosensors & Bioelectronics
Naveen K Singh, Phurpa Dema Thungon, Pedro Estrela, Pranab Goswami
There has been a continuous strive to develop portable, stable, sensitive and low cost detection system for malaria to meet the demand of effective screening actions in developing countries where the disease is most endemic. Herein, we report an aptamer-based field effect transistor (aptaFET) biosensor, developed by using an extended gate field effect transistor with inter-digitated gold microelectrodes (IDµE) for the detection of the malaria biomarker Plasmodium falciparum glutamate dehydrogenase (PfGDH) in serum samples...
September 26, 2018: Biosensors & Bioelectronics
Bayu Tri Murti, Athika Darumas Putri, Suvardhan Kanchi, Myalowenkosi I Sabela, Krishna Bisetty, Inamuddin, Abdullah M Asiri
Owing to the emerging applications of DNA-functionalized TiO2 nanocrystals towards DNA damage detection, it is inevitable to understand the better chemistry as well as in-depth molecular interaction phenomena. Fundamentally, energy difference underlies the layer-by-layer construction, resulted in the increase of the interaction energy and thus, altering the electrochemical behavior. Herein, Density functional theory (DFT) calculations were performed using DMol3 and DFTB+ codes successfully to elucidate the structural, electronics, and vibrational properties of the layer-by-layer components composing ss-DNA/dopamine/TiO2 /FTO...
November 2018: Journal of Photochemistry and Photobiology. B, Biology
Andrea Cerea, Valeria Caprettini, Giulia Bruno, Laura Lovato, Giovanni Melle, Francesco Tantussi, Rosario Capozza, Fabio Moia, Michele Dipalo, Francesco De Angelis
Biological studies on in vitro cell cultures are of fundamental importance to investigate cell response to external stimuli, such as new drugs for the treatment of specific pathologies, or to study communication between electrogenic cells. Although three-dimensional (3D) nanostructures brought tremendous improvements on biosensors used for various biological in vitro studies, including drug delivery and electrical recording, there is still a lack of multifunctional capabilities that could help gain deeper insights in several bio-related research fields...
October 11, 2018: Lab on a Chip
Fereshte Gholami, Aso Navaee, Abdollah Salimi, Rezgar Ahmadi, Azam Korani, Rahman Hallaj
Bipolar electrochemistry (BPE) has been lately explored as a simple, reliable and novel electrochemical technique for the adjustment of various conductive substrates. Herein, BPE is performed to derive both of cathode and anode electrodes for the development of mediatorless/membraneless biofuel cell (BFC). On one hand, a preferable substrate for immobilization of bilirubin oxidase enzyme is prepared based on the electropolymerization of thiophene-3-carboxcylic acid (TCA) on an Au microfilm as a bipolar electrode...
October 10, 2018: Scientific Reports
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