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https://www.readbyqxmd.com/read/30551175/crispr-c-circularization-of-genes-and-chromosome-by-crispr-in-human-cells
#1
Henrik Devitt Møller, Lin Lin, Xi Xiang, Trine Skov Petersen, Jinrong Huang, Luhan Yang, Eigil Kjeldsen, Uffe Birk Jensen, Xiuqing Zhang, Xin Liu, Xun Xu, Jian Wang, Huanming Yang, George M Church, Lars Bolund, Birgitte Regenberg, Yonglun Luo
Extrachromosomal circular DNA (eccDNA) and ring chromosomes are genetic alterations found in humans with genetic disorders. However, there is a lack of genetic engineering tools to recapitulate and study the biogenesis of eccDNAs. Here, we created a dual-fluorescence biosensor cassette, which upon the delivery of pairs of CRISPR/Cas9 guide RNAs, CRISPR-C, allows us to study the biogenesis of a specific fluorophore expressing eccDNA in human cells. We show that CRISPR-C can generate functional eccDNA, using the novel eccDNA biosensor system...
December 14, 2018: Nucleic Acids Research
https://www.readbyqxmd.com/read/30551064/antibody-recognition-by-a-novel-microgel-photonic-crystal
#2
Na Sai, Zhong Sun, Yuntang Wu, Guowei Huang
In this study, a easy-to-prepare biosensor for the sensitive detection of the antibody (Ab) protein was developed using a novel microgel photonic crystal (MPC). The MPC was fabricated by the spin-coated self-assembly method with the monodisperse Ab-sensitive poly (methyl methacrylate-acrylamide-glutaraldehyde-hapten) (P(MMA-AM-GA-HP)) microgels. Morphology characterization showed that the P(MMA-AM-GA-HP) microgels possessed round shapes and the large specific surface area, and the formed MPC had a highly ordered three dimensional (3D) periodically-ordered structure with the desired structural color...
December 5, 2018: Bioorganic Chemistry
https://www.readbyqxmd.com/read/30551062/biosensing-methods-for-determination-of-creatinine-a-review
#3
REVIEW
C S Pundir, Parveen Kumar, Ranjana Jaiwal
Creatinine is a metabolic product of creatine phosphate in muscles, which provides energy to muscle tissues. Creatinine has been considered as indicator of renal function specifically after dialysis, thyroid malfunction and muscle damage. The normal level of creatinine in the serum and its excretion through urine in apparently healthy individuals is 45-140 μM and 0.8-2.0 gm/day respectively. The level of creatinine reaches >1000 μM in serum during renal, thyroid and kidney dysfunction or muscle disorder...
November 25, 2018: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/30550984/rapid-determination-of-1-3-propanediol-in-fermentation-process-based-on-a-novel-surface-enhanced-raman-scattering-biosensor
#4
Wei Chen Zhao, Hai Rui Ren, Xin Zhang, Zheng Wang, Yong Mei Zhao, Luo Liu, Zheng Long Wu, Hai Jun Xu
The production of 1,3-propanediol (1,3-PDO) is an important fermentation process. However, 1,3-PDO could not be distinguished separately and efficiently in fermentations previously because it has a highly similar molecular structure to the feedstock glycerol (GLY) and by-product lactic acid (Lac), which leads to the difficulty of quantification. In this paper, a low-cost and environmentally friendly biosensor based on surface-enhanced Raman scattering (SERS) technique was developed. Using it, the concentration of 1,3-PDO and Lac in a fermentation solution can be determined directly from their respective characteristic peaks in Raman spectroscopy...
November 28, 2018: Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy
https://www.readbyqxmd.com/read/30550731/a-novel-catalase-mimicking-nanocomposite-of-mn-ii-poly-l-histidine-carboxylated-multi-walled-carbon-nanotubes-and-the-application-to-hydrogen-peroxide-sensing
#5
Jiexin Zhou, Yuan Chen, Lintao Lan, Cong Zhang, Meixin Pan, Yingying Wang, Bingkai Han, Zihua Wang, Jun Jiao, Qiang Chen
In this work, a novel enzyme-mimicking nanocomposite of Mn(II)-poly-L-histidine (PLH) functionalized carboxylated multi walled carbon nanotubes (CMWCNTs) was designed and synthesized. Based on the catalase-like activity of the nanocomposite, a non-enzymatic hydrogen peroxide (H2O2) biosensor was then established and explored for H2O2 electrochemical detection. The nanocomposite was characterized by Fourier transform infrared spectra, Raman spectroscopy, and transmission electron microscopy. Due to the enlarged effective surface area and the efficient electrocatalytic activity of the Mn(II)-PLH redox-active units, the obtained Mn(II)-PLH-CMWCNT electrode showed excellent electrocatalytic performance toward H2O2 disproportionation...
December 11, 2018: Analytical Biochemistry
https://www.readbyqxmd.com/read/30550559/dissecting-the-regulation-and-function-of-atp-at-the-single-cell-level
#6
Jianhan Zhang, Xu Han, Yihan Lin
Regulation of cellular ATP level is critical for diverse biological processes and may be defective in diseases such as cancer and mitochondrial disorders. While mitochondria play critical roles in ATP level regulation, we still lack a systematic and quantitative picture of how individual mitochondrial-related genes contribute to cellular ATP level and how dysregulated ATP levels may affect downstream cellular processes. Advances in genetically encoded ATP biosensors have provided new opportunities for addressing these issues...
December 14, 2018: PLoS Biology
https://www.readbyqxmd.com/read/30550275/genetically-encoded-fluorescent-biosensors-illuminate-the-spatiotemporal-regulation-of-signaling-networks
#7
Eric C Greenwald, Sohum Mehta, Jin Zhang
Cellular signaling networks are the foundation which determines the fate and function of cells as they respond to various cues and stimuli. The discovery of fluorescent proteins over 25 years ago enabled the development of a diverse array of genetically encodable fluorescent biosensors that are capable of measuring the spatiotemporal dynamics of signal transduction pathways in live cells. In an effort to encapsulate the breadth over which fluorescent biosensors have expanded, we endeavored to assemble a comprehensive list of published engineered biosensors, and we discuss many of the molecular designs utilized in their development...
December 14, 2018: Chemical Reviews
https://www.readbyqxmd.com/read/30548750/fluorescent-biosensor-for-detection-of-the-r248q-aggregation-prone-mutant-of-p53
#8
Morgan Pellerano, Delphine Naud-Martin, Florence Mahuteau-Betzer, Marie Morille, May Catherine Morris
The p53 tumour suppressor and guardian of the genome undergoes missense mutations which lead to functional inactivation in 50% human cancers. These mutations occur mostly in the DNA-binding domain of the protein and several of these induce conformational changes which lead to amyloid-like protein aggregation. Here we describe a fluorescent biosensor that reports on the R248Q mutant of p53 in vitro and in living cells, engineered through conjugation of an environmentally-sensitive probe onto a peptide derived from the primary aggregation segment of p53...
December 13, 2018: Chembiochem: a European Journal of Chemical Biology
https://www.readbyqxmd.com/read/30547579/dual-wavelength-ratiometric-electrochemiluminescence-immunosensor-for-cardiac-troponin-i-detection
#9
Jing Ye, Liping Zhu, Mengxia Yan, Qiuju Zhu, Qingqing Lu, Jianshe Huang, Hua Cui, Xiurong Yang
Ratiometric electrochemiluminescence (ECL) has attracted special focus in biological analysis field because it could eliminate the environmental interference to gain precise measurement. Herein, a dual-wavelength ratiometric ECL biosensor was designed for detection of cardiac troponin I (cTnI), where (4,4'-dicarboxylicacid-2,2'-bipyridyl) ruthenium(II) (Ru(dcbpy)32+) and Au nanoparticles loaded graphene oxide/polyethyleneimine (GPRu-Au) nanomaterial acts as an acceptor, and Au nanoparticles modified graphitic phase carbon nitride nanosheets composite (Au-CNN) acts as donor...
December 14, 2018: Analytical Chemistry
https://www.readbyqxmd.com/read/30547109/long-segment-detection-of-htlv-1-genome-based-on-the-fluorescence-quenching-technique
#10
Mohadeseh Zarei Ghobadi, Sayed-Hamidreza Mozhgani, Fatemeh Hakimian, Mehdi Norouzi, Seyed Abdolrahim Rezaee, Hedayatollah Ghourchian
Detecting fluorescence changes due to energy transfer between a quencher and fluorophore is a common method used for the fluorescence-based biosensors. In the present report, a new biosensor for long segment detection of the human T cell-lymphotropic virus 1 genome was constructed based on the fluorescence quenching of graphene oxide by gold nanoparticles. The fluorescence signal of unmodified graphene oxide was measured before and after hybridization of target and probes functionalized with gold nanoparticles...
December 2018: Heliyon
https://www.readbyqxmd.com/read/30546076/an-automated-bacterial-concentration-and-recovery-system-for-pre-enrichment-required-in-rapid-escherichia-coli-detection
#11
Yushan Zhang, Chang-Qing Xu, Tianyi Guo, Lingcheng Hong
One of the biggest challenges in rapid low concentration bacterial detection is the pre-concentration or pre-enrichment, which aims to increase bacteria concentration and reduce sample volume for easy bacterial detection. In practical bacterial detection, large-volume water samples with a pathogenic bacterial concentration of less than 1 CFU/mL have to be tested rapidly. The reported biosensors either have insufficient detection limit or have limited capability of handling a sufficiently large water sample...
December 13, 2018: Scientific Reports
https://www.readbyqxmd.com/read/30545762/label-free-optical-biosensor-for-target-detection-based-on-simulation-assisted-catalyzed-hairpin-assembly
#12
Yingying Zhang, Luhui Wang, Yanan Wang, Yafei Dong
The development of efficient and convenient strategy without involving enzyme or complex nanomaterial for the micro molecules detection has profound meaning in the diagnosis of diseases. Herein, taking the advantages of the strong affinity of aptamer and catalyzed hairpin assembly, we develop a new non-label optical amplified strategy for thrombin detection in this work. To support both biological inquiry and technological innovation, thermodynamic models are introduced to predict the minimum energy secondary structure of interacting nucleic acid strands and calculate the partition function and equilibrium concentration for complexes in our system...
December 3, 2018: Computational Biology and Chemistry
https://www.readbyqxmd.com/read/30545415/current-state-and-future-directions-of-technology-based-ecological-momentary-assessments-and-interventions-for-major-depressive-disorder-protocol-for-a-systematic-review
#13
Desirée Colombo, Azucena Garcia Palacios, Javier Fernandez Alvarez, Andrea Patané, Michelle Semonella, Pietro Cipresso, Marta Kwiatkowska, Giuseppe Riva, Cristina Botella
BACKGROUND: Ecological momentary assessments (EMAs) and ecological momentary interventions (EMIs) represent a novel approach for the assessment and delivery of psychological support to depressed patients in daily life. Beyond the classical paper-and-pencil daily diaries, the more recent progresses in Information and Communication Technologies (ICT) enabled researchers to bring all the needed processes together in only one device, i.e., response signaling, repeated symptom collection, information storage, secure data transfer, and psychological support delivery...
December 13, 2018: Systematic Reviews
https://www.readbyqxmd.com/read/30544947/application-of-real-time-cell-electronic-analysis-system-in-modern-pharmaceutical-evaluation-and-analysis
#14
REVIEW
Guojun Yan, Qian Du, Xuchao Wei, Jackelyn Miozzi, Chen Kang, Jinnv Wang, Xinxin Han, Jinhuo Pan, Hui Xie, Jun Chen, Weihua Zhang
Objective: We summarized the progress of the xCELLigence real-time cell analysis (RTCA) technology application in recent years for the sake of enriching and developing the application of RTCA in the field of Chinese medicine. Background: The RTCA system is an established electronic cellular biosensor. This system uses micro-electronic biosensor technology that is confirmed for real-time, label-free, dynamic and non-offensive monitoring of cell viability, migration, growth, spreading, and proliferation. Methods: We summarized the relevant experiments and literature of RTCA technology from the principles, characteristics, applications, especially from the latest application progress...
December 11, 2018: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/30544883/recent-advances-in-aiv-biosensors-composed-of-nanobio-hybrid-material
#15
REVIEW
Taek Lee, Jae-Hyuk Ahn, Sun Yong Park, Ga-Hyeon Kim, Jeonghyun Kim, Tae-Hyung Kim, Inho Nam, Chulhwan Park, Min-Ho Lee
Since the beginning of the 2000s, globalization has accelerated because of the development of transportation systems that allow for human and material exchanges throughout the world. However, this globalization has brought with it the rise of various pathogenic viral agents, such as Middle East respiratory syndrome coronavirus (MERS-CoV), severe acute respiratory syndrome coronavirus (SARS-CoV), Zika virus, and Dengue virus. In particular, avian influenza virus (AIV) is highly infectious and causes economic, health, ethnical, and social problems to human beings, which has necessitated the development of an ultrasensitive and selective rapid-detection system of AIV...
December 9, 2018: Micromachines
https://www.readbyqxmd.com/read/30544696/development-of-dna-pair-biosensor-for-quantization-of-nuclear-factor-kappa-b
#16
Zhaohui Wang, Pak Kin Wong
Nuclear factor kappa B (NF-κB), regulating the expression of several genes that mediate the inflammatory responses and cell proliferation, is one of the therapeutic targets for chronic inflammatory disease and cancer. A novel molecular binding scheme for the detection of NF-κB was investigated for its affinity to Ig-κB DNA composed by dye and quencher fluorophores, and this specificity is confirmed by competing with the DNA sequence that is complementary to the Ig-κB DNA. We create a normalization equation to remove the negative effects from the various initial fluorophore concentrations and the background noise...
December 10, 2018: Biosensors
https://www.readbyqxmd.com/read/30544083/microfluidic-chip-coupled-with-optical-biosensors-for-simultaneous-detection-of-multiple-analytes-a-review
#17
REVIEW
Zerong Liao, Yang Zhang, Yongrui Li, Yunfei Miao, Shimeng Gao, Fankai Lin, Yulin Deng, Lina Geng
This article reviews the recent advances in microfluidic-chip integrated optical biosensors for simultaneous detection of multiple analytes. In particular, the principles and recent progress in different kinds of multiplex optical biosensors and their biological application were reviewed comprehensively. Sensors based on multiplexed detection have absolute advantages in analysis throughput than single assay. The microfluidic chip, a type of micro-total analysis system (µTAS), provides an ideal platform for integration of high-throughput biosensors...
November 20, 2018: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/30544070/a-remarkable-thermosensitive-hydrogel-cross-linked-by-two-inorganic-nanoparticles-with-opposite-charges
#18
Shuo Wang, Zhaofu Zhang, Linfeng Dong, Geoffrey I N Waterhouse, Qihai Zhang, Lifang Li
Herein, we report the successful synthesis of a series of poly (N-isopropylacrylamide) (PNIPA)/layered double hydroxides (LDHs)/nano-hydroxyapatite (nano-HA) hydrogels via in-situ radical polymerization. The internal morphology, thermo sensitivity, rheological properties, swelling behavior and hemocompatibility of the PNIPA/LDHs/HA composite hydrogels were systematically investigated. Results show that the hydrogels had a reversible sol-gel transformation around 33 °C. Interactions between the positively charged LDHs and negatively charged nano-HA particles created a highly porous hydrogel network...
December 4, 2018: Journal of Colloid and Interface Science
https://www.readbyqxmd.com/read/30543816/cholesterol-biosensors-a-review
#19
REVIEW
Vinay Narwal, Ritu Deswal, Bhawna Batra, Vijay Kalra, Ritu Hooda, Minakshi Sharma, J S Rana
Cholesterol is the most important sterol synthesized by most of the human cells majorly in the liver. It is a necessary constituent of cell membranes, it acts as a precursor for the synthesis of steroid hormones, vitamin D, and bile acids. Cholesterol is transported in plasma primarily in the form of low-density lipoproteins (LDL), the principal route for its removal from tissues to the liver is in high-density lipoproteins (HDL), followed by excretion in the bile. Cholesterol level is less than 200 mg/dL in healthy persons...
December 10, 2018: Steroids
https://www.readbyqxmd.com/read/30543292/dual-channel-photoelectrochemical-ratiometric-aptasensor-with-up-converting-nanocrystals-using-spatial-resolved-technique-on-homemade-3d-printed-device
#20
Zhenli Qiu, Jian Shu, Jingfeng Liu, Dianping Tang
A near-infrared light (NIRL)-activated ratiometric photoelectrochemical (PEC) aptasensor was fabricated for detection of carcinoembryonic antigen (CEA) coupling with upconversion nanoparticles (UCNPs)-semiconductor nanocrystals-based spatial-resolved technique on a homemade 3D printing device in which a self-regulating integrated electrode was designed for dual signal readout. The as-prepared NaYF4:Yb, Er UCNPs@CdTe nanocrystals were initially assembled on two adja-cent photoelectrodes, then CEA aptamer 1 (A1) and capture DNA (CA) were modified onto two working photoelectrodes (WP1 and WP2) through covalent binding, respectively, and then gold nanoparticle-labeled CEA aptamer 2 (Au NP-A2) were immobilized on the surface of functional WP2 for the formation of double-stranded DNA...
December 13, 2018: Analytical Chemistry
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