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King-Wah Chiu, Ting-Lung Lin, Chee-Chien Yong, Chih-Che Lin, Yu-Fan Cheng, Chao-Long Chen
RATIONALE: According to previously published studies, major complications arising from a percutaneous liver biopsy are rare and occur in less than 0.1% of cases. This report describes an approach to percutaneous liver biopsy that can help avoid damage to the liver in a living donor liver transplantation (LDLT) setting. PATIENT CONCERNS: Case 1: In the first case a donor percutaneous liver biopsy (PLB) of both lobes of the liver was performed for pre-LDLT evaluation...
October 2018: Medicine (Baltimore)
Ben Fm Wijnen, Ruben Plb Van Engelen, Jan Ostermann, Axel Muhlbacher, Arnolf Fw Hendriks, Rafael Conde, Javier Gr Gonzalez, Mark Jg Govers, Silvia Maa Evers, Mickaël Hiligsmann
BACKGROUND: To assess patients' preferences for HIV testing in Colombia. METHODS: A discrete-choice experiment was used to assess preferences of patients diagnosed with HIV, for HIV testing in two HIV clinics in Bogotá, Colombia. Patients were asked to choose repeatedly between two hypothetical HIV testing options that varied with respect to five attributes: distance to testing site, confidentiality, testing days, sample collection method and the services if HIV positive...
October 3, 2018: Expert Review of Pharmacoeconomics & Outcomes Research
Anna Del Poggio, Luca Facchetti, Alessandra Ranza, Fabio Facchetti, Ugo Pazzaglia, Maria Pia Bondioni
Primary lymphoma of bone (PLB) is a rare entity, defined as a lymphoma confined to the bone without evidence of systemic involvement. The disease commonly affects middle-aged to elderly population and it accounts for less than 1% of all malignant lymphomas. We present a case of a 10-year-old child affected by PLB of the forearm and the frontal bone. Characteristic imaging features of PLB and the main differential diagnosis were discussed.
December 2018: Radiology Case Reports
Mu Chen, Dongzhu Xu, Adonis Z Wu, Evangelia Kranias, Shien-Fong Lin, Peng-Sheng Chen, Zhenhui Chen
AIMS: Phospholamban (PLB) is the key regulator of the cardiac Ca2+ pump (SERCA2a)-mediated sarcoplasmic reticulum Ca2+ stores. We recently reported that PLB is highly concentrated in the nuclear envelope (NE) from where it can modulate perinuclear Ca2+ handling of the cardiomyocytes (CMs). Since inositol 1,4,5-trisphosphate (IP3 ) receptor (IP3 R) mediates nuclear Ca2+ release, we examined whether the nuclear pool of PLB regulates IP3 -induced nuclear Ca2+ handling. METHODS AND RESULTS: Fluo-4 based confocal Ca2+ imaging was performed to measure Ca2+ dynamics across both nucleus and cytosol in saponin-permeabilized CMs isolated from wild-type (WT) or PLB-knockout (PLB-KO) mice...
October 2018: Journal of Molecular and Cellular Cardiology
Hideaki Fukuda, Shigehiro Asai, Izumi Kanisawa, Tatsuya Takahashi, Takahiro Ogura, Hiroki Sakai, Kenji Takahashi, Akihiro Tsuchiya
PURPOSE: The purpose of this study was to evaluate the signal/noise quotient (SNQ) for graft maturation and the serial changes observed in the magnetic resonance imaging (MRI) findings after double-bundle (DB) anterior cruciate ligament (ACL) reconstruction using a hamstring tendon autograft at a minimum of 5 years after surgery. METHODS: Forty-five patients who underwent DB ACL reconstruction between 2007 and 2010 were included in this prospective study. All participants underwent postoperative MRI at 3 weeks and 3, 6, 9 and 12, 18, 24, 36, 48 and 50 months...
September 8, 2018: Knee Surgery, Sports Traumatology, Arthroscopy: Official Journal of the ESSKA
Emily Chen, Bruna Benso, Dalia Seleem, Luiz Eduardo Nunes Ferreira, Silvana Pasetto, Vanessa Pardi, Ramiro Mendonça Murata
Current treatments for Candida albicans infection are limited due to the limited number of antifungal drugs available and the increase in antifungal resistance. Curcumin is used as a spice, food preservative, flavoring, and coloring agent that has been shown to have many pharmacological activities. Thus, this study evaluated the modulatory effects of curcumin on major virulence factors associated with the pathogenicity of C. albicans . The minimum inhibitory concentration (MIC) of curcumin against C. albicans (SC5314) was determined...
2018: International Journal of Dentistry
Daniel R Stroik, Samantha L Yuen, Kevyn A Janicek, Tory M Schaaf, Ji Li, Delaine K Ceholski, Roger J Hajjar, Razvan L Cornea, David D Thomas
We have developed a structure-based high-throughput screening (HTS) method, using time-resolved fluorescence resonance energy transfer (TR-FRET) that is sensitive to protein-protein interactions in living cells. The membrane protein complex between the cardiac sarcoplasmic reticulum Ca-ATPase (SERCA2a) and phospholamban (PLB), its Ca-dependent regulator, is a validated therapeutic target for reversing cardiac contractile dysfunction caused by aberrant calcium handling. However, efforts to develop compounds with SERCA2a-PLB specificity have yet to yield an effective drug...
August 22, 2018: Scientific Reports
Baisen Li, Xiuhui Gu, Mingbo Wu, Ye Zhao, Jie Yang, Li Feng, Junjie Gou, Linyi Chen, Ting Li, Linpeng Li, Lan Wang, Li Zhu, Kun Zhang
Plumbagin (PLB) is the primary component of the traditional Chinese medicine Baihua Dan, and possesses anti-infection and anticancer effects, with the ability to enhance the sensitivity of tumor cells to radiation therapy. However, the anticancer effect of PLB on nasopharyngeal carcinoma and the underlying mechanisms remain unclear. In this study, we investigated the anticancer effects of PLB on nasopharyngeal carcinoma 6-10B cells and clarified its molecular mechanisms in vitro. The results showed that PLB was effective against 6-10B cells proliferation in a dose-dependent manner by inducing G2/M phase cell cycle arrest...
October 2018: Anti-cancer Drugs
George R R Bell, Dean E Natwick, Sean R Collins
Neutrophils are key early responders in the innate immune response that use chemotaxis, the directed migration along chemical gradients, to reach sites of infection or inflammation. This process requires integrating inputs from cell surface receptors with the cell's polarity and motility signaling network, in which highly dynamic and interconnected signaling by Rho-family GTPases plays a central role. To understand this fundamentally important behavior, we describe a high-resolution, under-agarose chemotaxis assay for use with neutrophil-like cell lines (HL-60 or PLB-985) or with primary neutrophils...
2018: Methods in Molecular Biology
Tatiana M Vinogradova, Syevda Tagirova Sirenko, Edward G Lakatta
Spontaneous beating of the heart pacemaker, the sinoatrial node, is generated by sinoatrial node cells (SANC) and caused by gradual change of the membrane potential called diastolic depolarization (DD). Submembrane local Ca2+ releases (LCR) from sarcoplasmic reticulum (SR) occur during late DD and activate an inward Na⁺/Ca2+ exchange current, which accelerates the DD rate leading to earlier occurrence of an action potential. A comparison of intrinsic SR Ca2+ cycling revealed that, at similar physiological Ca2+ concentrations, LCRs are large and rhythmic in permeabilized SANC, but small and random in permeabilized ventricular myocytes (VM)...
July 25, 2018: International Journal of Molecular Sciences
Antonio F Melo Junior, Polyana L M Dalpiaz, Glauciene J Sousa, Phablo Wendell C Oliveira, Antônio M Birocale, Tadeu U Andrade, Gláucia R Abreu, Nazaré S Bissoli
AIMS: Hypertension is a highly prevalent disease that has been correlated to severe organ damage and mortality. However, the role of androgens in hypertension is controversial. The aim of this study was to evaluate the cardiac effects of the nandrolone decanoate (NDL) in male SHR. MAIN METHODS: At 12 weeks of age, male SHR rats were separated into three groups: Control (CON), Nandrolone 10 mg/kg twice weekly (NDL), and NDL plus Enalapril 10 mg/kg/day (NDL-E) groups...
September 1, 2018: Life Sciences
Bo Sun, Jinhong Wei, Xiaowei Zhong, Wenting Guo, Jinjing Yao, Ruiwu Wang, Alexander Vallmitjana, Raul Benitez, Leif Hove-Madsen, S R Wayne Chen
Sarcoplasmic reticulum (SR) Ca2+ cycling is governed by the cardiac ryanodine receptor (RyR2) and SR Ca2+ -ATPase (SERCA2a). Abnormal SR Ca2+ cycling is thought to be the primary cause of Ca2+ alternans that can elicit ventricular arrhythmias and sudden cardiac arrest. Although alterations in either RyR2 or SERCA2a function are expected to affect SR Ca2+ cycling, whether and to what extent altered RyR2 or SERCA2a function affects Ca2+ alternans is unclear. Here, we employed a gain-of-function RyR2 variant (R4496C) and the phospholamban-knockout (PLB-KO) mouse model to assess the effect of genetically enhanced RyR2 or SERCA2a function on Ca2+ alternans...
August 31, 2018: Journal of Biological Chemistry
Patricia Munsch-Alatossava, Reijo Käkelä, Dominique Ibarra, Mohammed Youbi-Idrissi, Tapani Alatossava
Cold storage aims to preserve the quality and safety of raw milk from farms to dairies; unfortunately, low temperatures also promote the growth of psychrotrophic bacteria, some of which produce heat-stable enzymes that cause spoilage of milk or dairy products. Previously, N2 gas flushing of raw milk has demonstrated significant potential as a method to hinder bacterial growth at both laboratory and pilot plant scales. Using a mass spectrometry-based lipidomics approach, we examined the impact of cold storage [at 6°C for up to 7 days, the control condition (C)], on the relative amounts of major phospholipids (phosphatidylethanolamine/PE, phosphatidylcholine/PC, phosphatidylserine/PS, phosphatidylinositol/PI, and sphingomyelin/SM) in three bovine raw milk samples, and compared it to the condition that received additional N2 gas flushing (N)...
2018: Frontiers in Microbiology
Fei Hong, Huan-Xin Zhang, Chong Chen, Zhi-Ling Yan, Qing-Yun Wu, Ling-Yu Zeng, Zheng-Yu Li, Kai-Lin Xu
OBJECTIVE: To investigate the effect of steadily down-regulating the expression of VE-cadherin on the chemotheraputic sensitivity of K562 cells, and explore its possible mechanism. METHODS: Specifically targeting interference sequences carrying human VE-cadherin were designed, the recombinant lentiviral vector containing the IRES-GFP and NEO segment was constructed; recombinant lentivirus was generated by three-plasmids packing system, and transfected into K562 cells, then the cells steadily down-regulated were sorted...
June 2018: Zhongguo Shi Yan Xue Ye Xue za Zhi
Eli Fernández-de Gortari, L Michel Espinoza-Fonseca
Sarcoplasmic reticulum Ca2+ -ATPase (SERCA) is critical for cardiac Ca2+ transport. Reversal of phospholamban (PLB)-mediated SERCA inhibition by saturating Ca2+ conditions operates as a physiological rheostat to reactivate SERCA function in the absence of PLB phosphorylation. Here, we performed extensive atomistic molecular dynamics simulations to probe the structural mechanism of this process. Simulation of the inhibitory complex at superphysiological Ca2+ concentrations ([Ca2+ ] = 10 mm) revealed that Ca2+ ions interact primarily with SERCA and the lipid headgroups, but not with PLB's cytosolic domain or the cytosolic side of the SERCA-PLB interface...
August 10, 2018: Journal of Biological Chemistry
Leonardo Bocchi, Monia Savi, Valeria Naponelli, Rocchina Vilella, Gianluca Sgarbi, Alessandra Baracca, Giancarlo Solaini, Saverio Bettuzzi, Federica Rizzi, Donatella Stilli
BACKGROUND/AIMS: Dietary polyphenols from green tea have been shown to possess cardio-protective activities in different experimental models of heart diseases and age-related ventricular dysfunction. The present study was aimed at evaluating whether long term in vivo administration of green tea extracts (GTE), can exert positive effects on the normal heart, with focus on the underlying mechanisms. METHODS: The study population consisted of 20 male adult Wistar rats...
2018: Cellular Physiology and Biochemistry
Tatiana M Vinogradova, Syevda Sirenko, Yevgeniya O Lukyanenko, Dongmei Yang, Kirill V Tarasov, Alexey E Lyashkov, Nevin J Varghese, Yue Li, Khalid Chakir, Bruce Ziman, Edward G Lakatta
BACKGROUND: Spontaneous firing of sinoatrial node cells (SANCs) is regulated by cAMP-mediated, PKA (protein kinase A)-dependent (cAMP/PKA) local subsarcolemmal Ca2+ releases (LCRs) from RyRs (ryanodine receptors). LCRs occur during diastolic depolarization and activate an inward Na+ /Ca2+ exchange current that accelerates diastolic depolarization rate prompting the next action potential. PDEs (phosphodiesterases) regulate cAMP-mediated signaling; PDE3/PDE4 represent major PDE activities in SANC, but how they modulate LCRs and basal spontaneous SANC firing remains unknown...
June 2018: Circulation. Arrhythmia and Electrophysiology
Peter D Martin, Zachary M James, David D Thomas
We have used site-directed spin labeling and electron paramagnetic resonance (EPR) to map interactions between the transmembrane (TM) domains of the sarcoplasmic reticulum Ca2+ -ATPase (SERCA) and phospholamban (PLB) as affected by PLB phosphorylation. In the cardiac sarcoplasmic reticulum, PLB binding to SERCA results in Ca-dependent enzyme inhibition, which is reversed by PLB phosphorylation at Ser16. Previous spectroscopic studies on SERCA-PLB have largely focused on the cytoplasmic domain of PLB, showing that phosphorylation induces a structural shift in this domain relative to SERCA...
June 5, 2018: Biophysical Journal
Ian Warbrick, Simon W Rabkin
Heart failure with preserved ejection fraction (HFpEF) represents an important cardiac condition because of its increasing prevalence, resistance to treatment and high associated morbidity and mortality. Two of the major mechanisms responsible for HFpEF are impaired cardiomyocyte sarcoplasmic reticulum (SR) Ca2+ ATPase (SERCA2a), which is responsible for calcium reuptake into the SR, and cardiac fibroblasts/myofibroblasts that produce collagen or myocardial fibrosis. Phospholamban (PLB), in the SR and endoplasmic reticulum, is the primary regulator of SERCA2a in the heart and acts as a reversible inhibitor of SERCA2a...
May 25, 2018: Peptides
Andressa Heimbecher Soares, Nidia Celeste Horie, Lucas Augusto Piccinin Chiang, Bruno Caramelli, Mariana Gomes Matheus, Alexandre Holthausen Campos, Luciana Cavalheiro Marti, Fernanda Agostini Rocha, Marcio C Mancini, Elaine Maria Frade Costa, Cintia Cercato
BACKGROUND: Obesity causes secondary hypogonadism (HG) in men. Standard testosterone (T) replacement therapy improves metabolic parameters but leads to infertility. OBJECTIVE: To evaluate clomiphene citrate (CC) treatment of adult men with male obesity-associated secondary hypogonadism (MOSH). DESIGN: Single-center, randomized, double-blind, placebo-controlled trial. PARTICIPANTS: Seventy-eight men aged 36.5 ± 7...
June 2018: International Journal of Obesity: Journal of the International Association for the Study of Obesity
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