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Simultaneous Determination

Jian-Wei Dong, Xue-Jiao Li, Le Cai, Jun-You Shi, Yu-Feng Li, Cui Yang, Zhen-Jie Li
Stephania epigaea Lo is an important herbal medicine used as antiphlogistic and analgesic drugs. Its major components are dicentrine (1) and sinomenine (2). In the present study, a rapid, accurate, and precise method for simultaneous quantitation of dicentrine (1) and sinomenine (2) in S. epigeae using 1 H NMR spectra was developed. The deuterated solvent of DMSO-d6 enabled satisfactory separation of the signals to be integrated in 1 H NMR spectrum and dimethyl terephthalate was selected as an internal standard...
August 6, 2018: Journal of Pharmaceutical and Biomedical Analysis
Aoife Healy, Roozbeh Naemi, Lakshmi Sundar, Panagiotis Chatzistergos, Ambady Ramachandran, Nachiappan Chockalingam
AIM: To validate the paper grip test for assessing plantar flexion strength of the hallux. METHODS: Plantar flexor strength for 69 people with diabetic neuropathy was assessed: (a) using the paper grip test while simultaneously a plantar pressure platform quantified the force under the hallux, and (b) using a hand-held dynamometer. Following testing, participants were divided into groups: (1) passed vs. failed paper grip test (2) males vs. females. Statistical analyses determined if differences were evident between the groups and assessed the relationship between the paper grip test and the hand-held dynamometer...
August 13, 2018: Diabetes Research and Clinical Practice
Madeline Harlow, Calum MacAulay, Pierre Lane, Anthony M D Lee
We recently demonstrated a new two-dimensional imaging paradigm called dual-beam manually actuated distortion-corrected imaging (DMDI). This technique uses a single mechanical scanner and two spatially separated beams to determine relative sample velocity and simultaneously corrects image distortions due to manual actuation. DMDI was first demonstrated using a rotating dual-beam micromotor catheter. Here, we present a new implementation of DMDI using a single axis galvanometer to scan a pair of beams in approximately parallel lines onto a sample...
July 9, 2018: Optics Express
Jens G Brockmann, Amir Butt, Hussa F Al Hussaini, Hadeel AlMana, Khaled AlSaad, Moheeb Al-Awwami, Dieter C Broering, Tariq Ali
BACKGROUND: Histological evaluation of the pancreas graft is usually done on demand resulting in significant delays. This analysis reports on endoscopic protocol duodenal graft biopsies at regular intervals to determine feasibility, safety and monitoring benefits. METHODS: Protocol duodenal graft biopsies in 27 18 consecutive pancreas transplants (10 simultaneous pancreas kidney [SPK], 17 pancreas after kidney [PAK]) with a follow-up of a minimum of 12 months were performed at days 14, 30, 90, 180, 360, 430...
August 15, 2018: Transplantation
Dae-Hyun Nam, Oleksandr S Bushuyev, Jun Li, Phil De Luna, Ali Seifitokaldani, Cao-Thang Dinh, F Pelayo Garcia de Arquer, Yuhang Wang, Zhiqin Liang, Andrew H Proppe, Chih Shan Tan, Petar Todorovic, Osama Shekhah, Christine M Gabardo, Jea Woong Jo, Jongmin Choi, Min-Jae Choi, Se-Woong Baek, Junghwan Kim, David Sinton, Shana O Kelley, Mohamed Eddaoudi, Edward H Sargent
The electrochemical carbon dioxide reduction reaction (CO2 RR) produces diverse chemical species. Cu clusters with a judiciously-controlled surface coordination number (CN) provide active sites that simultaneously optimize selectivity, activity, and efficiency for CO2 RR. Here we report a strategy involving metal-organic framework (MOF)-regulated Cu cluster formation that shifts CO2 electroreduction toward multiple-carbon product generation. Specifically, we promoted undercoordinated sites during the formation of Cu clusters by controlling the structure of the Cu dimer, the precursor for Cu clusters...
August 16, 2018: Journal of the American Chemical Society
Scott Nathan Loewenstein, Stephen P Duquette, Joshua M Adkinson
BACKGROUND: Hand surgery dogma suggests that simultaneous surgical treatment of carpal tunnel syndrome (CTS) and Dupuytren's contracture (DC) results in an increased incidence of Complex Regional Pain Syndrome (CRPS). As a result, many surgeons do not perform surgery for the two conditions concurrently. Our goal was to determine the extent of this association. METHODS: We identified all patients undergoing surgical treatment for CTS, DC, or both between April 1982 and March 2017 using the Indiana Network for Patient Care (INPC), a large, multi-institutional, statewide information exchange...
August 11, 2018: Plastic and Reconstructive Surgery
Sander Rozemeijer, Jelle L G Haitsma Mulier, Jantine G Röttgering, Paul W G Elbers, Angélique M E Spoelstra-de Man, Pieter Roel Tuinman, Monique C de Waard, Heleen M Oudemans-van Straaten
INTRODUCTION: Shock is characterised by micro- and macrovascular flow impairment contributing to acute kidney injury (AKI). Routine monitoring of the circulation regards the macrocirculation but not the renal circulation which can be assessed with Doppler ultrasound as renal resistive index (RRI). RRI reflects resistance to flow. High RRI predicts persistent AKI. Study aims were to determine whether RRI is elevated in shock and to identify determinants of RRI. MATERIALS AND METHODS: This prospective observational cohort study included two cohorts of patients, with and without shock <24-h after intensive care admission...
August 14, 2018: Shock
Hu Jin, Alex I Finnegan, Jun Song
Nucleosomes form the fundamental building blocks of eukaryotic chromatin, and previous attempts to understand the principles governing their genome-wide distribution have spurred much interest and debate in biology. In particular, the precise role of DNA sequence in shaping local chromatin structure has been controversial. This paper rigorously quantifies the contribution of hitherto-debated sequence features - including G+C content, 10.5-bp periodicity, and poly(dA:dT) tracts to three distinct aspects of genome-wide nucleosome landscape: occupancy, translational positioning and rotational positioning...
August 16, 2018: Physical Biology
Jill M Wecht, Joseph P Weir, Caitlyn G Katzelnick, Glenn Wylie, Mastanna Eraifej, Nhuguynh Nguyen, Trevor Dyson-Hudson, William A Bauman, Nancy Chiaravalotti
Cognitive deficits are prevalent in the spinal cord injury (SCI) population, and consensus suggests that concomitant traumatic brain injury or co-morbid conditions are primarily responsible for these deficits. However, mounting evidence supports the possibility that systemic and cerebral hemodynamic dysfunction may contribute to the cognitive deficits reported in persons with SCI. We sought to determine the contribution of changes in blood pressure (BP) and changes in cerebral blood flow velocity (CBFv) to test performance on the Symbol Digit Modalities Test (SDMT) in persons with SCI compared to matched nonSCI controls...
August 16, 2018: Journal of Neurotrauma
Lingzhi Kong, Zhi Wu, Huakun Zhao, Haomin Cui, Ji Shen, Jiang Chang, Haiyan Li, Yaohua He
Diabetic wound is hard to heal mainly because of the difficulty in vascularization in the wound area. Accumulating evidences have shown that desferrioxamine (DFO) can promote secretion of hypoxia inducible factor-1 (HIF-1α), thereby upregulating the expression of angiogenic growth factors and facilitating revascularization. Our preliminary study has demonstrated that Si ions in bioglass (BG) can upregulate vascular endothelial growth factor (VEGF) expression, thus promoting revascularization. It is hypothesized that the combined use of BG and DFO may have a synergistic effect in promoting VEGF expression and revascularization...
August 16, 2018: ACS Applied Materials & Interfaces
Noha M Hosny, Noha N Atia, Samia M El-Gizawy, Dalia M Badary, Mohammad S Hareedy
The present study was designed to evaluate the potential protective effects of the cysteinyl leukotriene receptor blocker montelukast (MNK) and the xanthine oxidase inhibitor febuxostat (FBX) and their combination on a model of acute gouty arthritis induced by intraarticular injection of monourate sodium crystal (MUC) injection in rats. Additionally, we established an HPTLC method for the quantitative determination of both drugs simultaneously and applied this method to detect this combination in rat plasma...
August 16, 2018: Analyst
Shiyu Li, Jingdong Peng, Fang Chen, Ziyu Pan, Huanjun Peng, Yu Chen, Yan He, Xiang Wang
A highly sensitive and selective method of high performance liquid chromatography (HPLC) combined with resonance Rayleigh scattering (RRS) spectra was developed for the detection of three antihistamine drugs, including pyrilamine (PY), carbinoxamine (CAR) and tripelennamine (TRI). The three antihistamines were separated by a C18 column. The mobile phase contained 25% acetonitrile (ACN) and 75% phosphate buffered solution (pH 3.2) with the flow rate of 0.4 ml min-1 . In medium of Britton-Robinson (BR) buffer solution (pH 4...
August 16, 2018: Luminescence: the Journal of Biological and Chemical Luminescence
Xiaolong Liu, Nils Y Meiresonne, Ahmed Bouhss, Tanneke den Blaauwen
Peptidoglycan (PG) is the unique cell shape-determining component of the bacterial envelope, and is a key target for antibiotics. PG synthesis requires the transmembrane movement of the precursor lipid II, and MurJ has been shown to provide this activity in E. coli. However, how MurJ functions in vivo has not been reported. Here we show that MurJ localizes both in the lateral membrane and at midcell, and is recruited to midcell simultaneously with late-localizing divisome proteins and proteins MraY and MurG...
August 15, 2018: Molecular Microbiology
Yan Nok Lau, Joseph Ng, Shan Yee Lee, Lam Chin Li, Cheuk Man Kwan, Sin Ming Fan, Bernard Pui Lam Leung, Chi Ngai Lo
BACKGROUND: In rheumatoid arthritis (RA) synovitis, activation of synoviocytes and infiltration of adaptive immune cells leads to synovial hyperplasia and joint swelling. Under the elevated extra-neural pressure, free nerve endings release neuropeptides, calcitonin gene-related peptide, and substance P, thus promoting neurogenic inflammation. OBJECTIVE: This study aimed to assess the effect of therapeutic neural mobilization (NM) exercises targeting the nervous system on disease impact in RA patients...
August 15, 2018: Zeitschrift Für Rheumatologie
Louis-Pierre Comeau, Derrick Y F Lai, Jane Jinglan Cui, Jodie Hartill
Ex-situ measurement of soil respiration is usually done with highly disturbed samples that may confound the interpretation and extrapolation of results. We have developed a lab respiration assessment method that better simulates field conditions and allows efflux estimations based on soil surface area. First, intact soil cores are extracted in the field and transferred to the lab. Next, soil moisture content and bulk density are assessed in each soil core. Immediately following this the soil cores are gently broken, pooled per treatment (or plot) and the root systems removed...
2018: MethodsX
Eva-Maria Ratai, Mohamad J Alshikho, Nicole R Zürcher, Marco L Loggia, Catherine L Cebulla, Paul Cernasov, Beverly Reynolds, Jennifer Fish, Raghav Seth, Suma Babu, Sabrina Paganoni, Jacob M Hooker, Nazem Atassi
Objective: To determine the relationship between brain tissue metabolites measured by in vivo magnetic resonance spectroscopy (1 H-MRS), and glial activation assessed with [11 C]-PBR28 uptake in people with amyotrophic lateral sclerosis (ALS). Methods: Forty ALS participants were evaluated clinically using the revised ALS functional rating scale (ALSFRS-R) and upper motor neuron burden (UMNB). All participants underwent simultaneous brain [11 C]-PBR28 PET and MR imaging including diffusion tensor imaging to acquire fractional anisotropy (FA)...
2018: NeuroImage: Clinical
Nai-Yuan Liu, Pinar Cay-Durgun, Tianmiao Lai, Mark Sprowls, Leslie Thomas, Mary Laura Lind, Erica Forzani
This paper introduces a wireless, solid-state, portable, and automated device capable of measuring the total ammonia [ammonia (NH3 ) and ammonium (NH4 + )] levels of fluids, including biological samples. This device reliably measures the total ammonia of biological samples (e.g., urine) faster than the current ammonia quantification techniques. Medical professionals typically estimate NH4+ levels using error-prone indirect measurement techniques (i.e., urine anion gap), which are time-consuming and are seldom suitable for periodic measurements...
2018: IEEE Journal of Translational Engineering in Health and Medicine
Bradley A Spicer, Ruby H P Law, Tom T Caradoc-Davies, Sue M Ekkel, Charles Bayly-Jones, Siew-Siew Pang, Paul J Conroy, Georg Ramm, Mazdak Radjainia, Hariprasad Venugopal, James C Whisstock, Michelle A Dunstone
Complement component 9 (C9) functions as the pore-forming component of the Membrane Attack Complex (MAC). During MAC assembly, multiple copies of C9 are sequentially recruited to membrane associated C5b8 to form a pore. Here we determined the 2.2 Å crystal structure of monomeric murine C9 and the 3.9 Å resolution cryo EM structure of C9 in a polymeric assembly. Comparison with other MAC proteins reveals that the first transmembrane region (TMH1) in monomeric C9 is uniquely positioned and functions to inhibit its self-assembly in the absence of C5b8...
August 15, 2018: Nature Communications
Ying Nie, Weifeng Yao
The quantitative analysis of multiple indexes remains an important quality evaluation method of traditional Chinese medicine (TCM) herbal formulas. The Chinese Pharmacopoeia 2015 only stipulates the content of a single component, specnuezhenide, in Erzhiwan composed of the Fructus Ligustri Lucidi (FLL) powder and aqueous extracts of Herba Ecliptae (HE). To generalize the intrinsic quality of Erzhiwan, a novel C30 -HPLC method with good precision, accuracy, and reproducibility was developed for the simultaneous determination of six compounds, including two isomers, and then an analytic hierarchy process was further applied to integrate and discriminate the quality of four samples prepared via different methods...
August 15, 2018: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
Miel Ap Vugts, Margot Cw Joosen, Agali Mert, Aglaia Me Zedlitz, Hubertus Jm Vrijhoef
BACKGROUND: Current evidence for the effectiveness of specialist multidisciplinary programs for burdensome chronic pain and functional somatic syndromes drives the effort to improve approaches, strategies, and delivery modes. It remains unknown to what extent and in what respect serious gaming during the regular outpatient rehabilitation can contribute to health outcomes. OBJECTIVE: The objectives of our study were to determine the effect of additional serious gaming on (1) physical and emotional functioning in general; (2) particular outcome domains; and (3) patient global impressions of change, general health, and functioning and to determine (4) the dependency of serious gaming effects on adherence...
August 15, 2018: Journal of Medical Internet Research
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