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Simultaneous Determination

Ewa Muszyńska, Mateusz Labudda, Ewa Hanus-Fajerska
The aim of present study was to evaluate the proteolytic response of metallicolous (M) and nonmetallicolous (NM) ecotypes of Alyssum montanum under heavy metals (HMs) stress. Therefore, shoot cultures of tested species grown on medium enriched simultaneously with 0.7 mM ZnSO4 , 3.0 μM Pb(NO3 )2 and 16.4 μM CdCl2 and these concentration corresponded to the content of their soluble forms marked in calamine substrate. After 8 weeks of cultivation, the overall protease activity (azocaseinolytic) and determination of the proteolytic (gelatinolytic) enzymes profile were estimated in HMs-treated and untreated specimens...
November 19, 2018: Journal of Plant Physiology
Steven L Matzner, Natalie Ronning, Jonathan Hawkinson, Tara Cummiskey, Jackson Buchanan, Emma Miller, Grady Carlisle
Two Phaseolus vulgaris L. cultivars were exposed to reduced water and stem mechanical perturbation treatments (flexing) to determine if acclimation to these treatments induced hydraulic changes, altered cavitation resistance and changed stem mechanical properties. Additionally, this study sought to determine if changes in cavitation resistance would support the pit area or conduit reinforcement hypotheses. Flexing reduced biomass, leaf area, xylem vessel area and hydraulic conductivity. One cultivar had greater measures of stem strength and cavitation resistance...
December 10, 2018: Physiologia Plantarum
Susana J Calderon, Caroline Mallory
Informed by grounded theory, this study explored adolescent oral health behaviors to determine what factors influence them and to develop a substantive theory explaining the social process behind the behaviors identified. Multiple types of data were collected and analyzed simultaneously: demographic and oral health questionnaires, 16 individual interviews, eight small group discussions, field notes, and investigator memoing. Participants were 37 Midwestern adolescents aged 13-18, and most were White or African-American females 14 years of age whose parents had at least a high school education...
December 9, 2018: Public Health Nursing
Yongzhi Tian, Yongqing Wen, Jinghan Sun, Longshan Zhao, Zhili Xiong, Feng Qin
A rapid, selective and sensitive ultra high performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) method was developed to simultaneously determine oxybutynin (OB) and its active metabolite N-desethyl oxybutynin (DEOB) in rat plasma. Sample of 0.1 mL plasma was extracted with n-hexane. Chromatographic separation was performed on a UPLC BEH C18 column(2.1 × 100 mm I.D.,1.7 μm) with mobile phase of methanol-water (containing 2 mmol/L ammonium acetate and 0.1% formic acid) (90:10, v/v). The detection was performed in positive selected reaction monitoring (SRM) mode...
December 7, 2018: Biomedical Chromatography: BMC
Li Zhang, Shaobin Li, Jianjiao Xin, Huiyuan Ma, Haijun Pang, Lichao Tan, Xinming Wang
A Cr-based metal-organic framework MIL-101(Cr) was used to load platinum nanoparticles (PtNPs) that were placed on a glassy carbon electrode (GCE). The modified GCE was used as a non-enzymatic xanthine sensor. Compared to bare GCE, it requires a strongly decreased working potential and an increased signal current for xanthine oxidation. This is due to the crystalline ordered structure and large specific surface of the MIL-101(Cr), and to the high conductivity of the Pt NPs. Differential pulse voltammetry (DPV) shows the sensor to have a wide linear range (0...
December 10, 2018: Mikrochimica Acta
Elizabeth A Bartlett, Mala Ananth, Samantha Rossano, Mengru Zhang, Jie Yang, Shu-Fei Lin, Nabeel Nabulsi, Yiyun Huang, Francesca Zanderigo, Ramin V Parsey, Christine DeLorenzo
PURPOSE: To determine if one venous blood sample can substitute full arterial sampling in quantitative modeling for multiple positron emission tomography (PET) radiotracers using simultaneous estimation of the input function (SIME). PROCEDURES: Participants underwent PET imaging with [11 C]ABP688, [11 C]CUMI-101, and [11 C]DASB. Full arterial sampling and additional venous blood draws were performed for quantification with the arterial input function (AIF) and SIME using one arterial or venous (vSIME) sample...
December 7, 2018: Molecular Imaging and Biology: MIB: the Official Publication of the Academy of Molecular Imaging
Fahimeh Zeraatkar Kashani, Sayed Mehdi Ghoreishi, Asma Khoobi, Morteza Enhessari
An electrochemical sensor is described for the simultaneous determination of ortho-hydroxybenzoic acid (OHB) and para-hydroxybenzoic acid (PHB). The sensor consists of a carbon paste electrode modified with nickel titanate nanoceramics (NiTiO3 /CPE). The NiTiO3 nanoceramics and the nanostructured modified CPE were characterized by X-ray diffraction, scanning electron microscopy, transmission electron microscopy, electrochemical impedance spectroscopy and cyclic voltammetry. Differential pulse voltammetry indicates that the response to OHB (best measured at 0...
December 10, 2018: Mikrochimica Acta
Derrick Y Tam, Harindra C Wijeysundera, Maral Ouzounian, Stephen E Fremes
OBJECTIVES: Our goal was to determine the range of perioperative mortality rates associated with the Ross procedure that results in a life expectancy similar to that seen with mechanical aortic valve replacement (mAVR) in young patients with aortic valve disease. METHODS: A fully probabilistic Markov microsimulation model with 1000 outer loops and 10 000 inner loops was constructed to compare gain in life expectancy and quality-adjusted life years between the index treatment with the Ross procedure versus mAVR for a theoretical cohort of young patients with aortic valve disease...
December 7, 2018: European Journal of Cardio-thoracic Surgery
Jae Han Ko, Suhan Lim, Young Han Lee, Ick Hwan Yang, Jin Hwa Kam, Kwan Kyu Park
Purpose: This study aimed to evaluate the efficacy of simultaneous computed tomography (CT) and quantitative CT (QCT) in patients with osteoporotic hip fracture (OHF) by analyzing the osteoporosis detection rate and physician prescription rate in comparison with those of conventional dual-energy X-ray absorptiometry (DXA). Materials and Methods: This study included consecutive patients older than 65 years who underwent internal fixation or hip arthroplasty for OHF between February and May 2015...
December 2018: Hip & Pelvis
Yi Su, Wei Li, Zhigang Huang, Ruozhong Wang, Weigui Luo, Qing Liu, Jianhua Tong, Langtao Xiao
Background: Abscisic acid (ABA) functions as a stress phytohormone in many growth and developmental processes in plants. The ultra-sensitive determination of ABA would help to better understand its vital roles and action mechanisms. Results: We report a new sensitive and high throughput quantitative real time immuno-PCR (qIPCR) method based on biotin-avidin linkage system for ABA determination in plants. ABA monoclonal antibody (McAb) coated on the inner surface of PCR well pretreated with glutaraldehyde...
2018: Plant Methods
Hongyin Ma, Zhen-Ni Guo, Hang Jin, Xiuli Yan, Jia Liu, Shan Lv, Peng Zhang, Xin Sun, Yi Yang
Background and Purpose: Dynamic cerebral autoregulation (dCA) is probably impaired in the acute and even subacute phases after acute ischemic stroke (AIS); however, the relationship between relevant clinical factors and dCA after AIS has not been investigated. The identification of possible determinants may therefore provide potential therapeutic targets to improve dCA in AIS. Methods: This study enrolled 67 consecutive patients diagnosed with AIS within 3 days from symptom onset. Serial measurements were performed 1-3 days (measurement 1) and 7-10 days (measurement 2) after the onset...
2018: Frontiers in Neurology
Annette Sterr, James K Ebajemito, Kaare B Mikkelsen, Maria A Bonmati-Carrion, Nayantara Santhi, Ciro Della Monica, Lucinda Grainger, Giuseppe Atzori, Victoria Revell, Stefan Debener, Derk-Jan Dijk, Maarten DeVos
Electroencephalography (EEG) recordings represent a vital component of the assessment of sleep physiology, but the methodology presently used is costly, intrusive to participants, and laborious in application. There is a recognized need to develop more easily applicable yet reliable EEG systems that allow unobtrusive long-term recording of sleep-wake EEG ideally away from the laboratory setting. cEEGrid is a recently developed flex-printed around-the-ear electrode array, which holds great potential for sleep-wake monitoring research...
2018: Frontiers in Human Neuroscience
Alexander I Kostyukov, Tomasz Tomiak
The two-segment model of the human arm is considered; the shoulder and elbow joint torques (JTs) are simulated, providing a slow, steady rotation of the force vector at any end-point of the horizontal working space. The sinusoidal waves describe the JTs, their periods coincide with that of the rotation, and phases are defined by the slopes of the correspondent lines from the joint axes to the end-point. Analysis of the JTs includes an application of the same discrete changes in one joint angle under fixation of the other one and vice versa; the JT pairs are compared for the "shoulder" and "elbow" end-point traces that pass under fixation of the elbow and shoulder angles, respectively...
2018: Frontiers in Neurorobotics
Shweta Pandey, Ravindra Kumar Garg, Hardeep Singh Malhotra, Neeraj Kumar, Ravi Uniyal
Guillain-Barré syndrome (GBS) is an infrequent neurological complication of dengue viral infection. It is broadly divided into either acute inflammatory demyelinating variety or an axonal variety (acute motor axonal and acute sensory and motor axonal variants). Axonal variants are distinctly infrequent. Two brothers (18 years and 15 years) were hospitalized on the same day with complaints of acute symmetric upper- and lower-limb weakness of 7 days' duration. They did not have overt manifestations of dengue fever which preceded the neurological presentation...
October 2018: Annals of Indian Academy of Neurology
José de Jesús Vidal-Mayo, Antonio Olivas-Martínez, Iván Pérez-Díaz, Juan M López-Navarro, Elizabet Sánchez-Landa, Eduardo Carrillo-Maravilla, Martha Portillo-González, Reynerio Fagundo-Sierra, Mario Andrés de Jesús Leal-Villareal
Background: Urine osmolarity (OsmU) is the gold standard for the evaluation of the kidney's urine concentration capacity; nevertheless, urinary density (UD) is often used as a surrogate for its estimation. Objective: The objective of this study was to analyze the accuracy of UD in estimating OsmU. Materials and Methods: A transversal study including patients with simultaneous determination of UD measured with refractometry and OsmU measured by osmometer (OsmUm)...
2018: Revista de Investigación Clínica; Organo del Hospital de Enfermedades de la Nutrición
Ehsan Ranaei Pirmardan, Zahra-Soheila Soheili, Shahram Samiei, Hamid Ahmadieh, Seyed Javad Mowla, Marzieh Naseri, Narsis Daftarian
Retinal degenerative diseases, due to the lack of regeneration systems and self-renewable cells, often lead to visual impairment. Pax6 is a pleiotropic transcription factor and its expression level determines self-renewal status or differentiation of retinal cells. Here, we investigated the fate of simultaneous induction of retinal ganglion cell death and Pax6 overexpression in retro-differentiation of retinal cells and their commitment to re-enter into the cell cycle. Induction of acute retinal ganglion cell death and generation of mouse experimental model was performed by N-methyl D-aspartic acid (NMDA) injection...
December 7, 2018: Scientific Reports
F I Ibragimov
AIM: To decrease disability and mortality in patients with severe combined pelvic and abdominal trauma via applications of new progressive treatment tactics. MATERIAL AND METHODS: Retrospective and prospective analysis included 1033 patients with severe combined traumas (SCT) who were treated at the Clinical Hospital #3 in 2009-2015. Patients were divided into 2 groups - control (2009-2010) and main (2011-2015) group. Treatment in control group was similar to conventional approaches...
2018: Khirurgiia
Alena-Svenja Wagner, Matthias Schumacher, Marcus Rohnke, Kristina Glenske, Michael Gelinsky, Stefan Arnhold, Sybille Mazurek, Sabine Wenisch
Given the important effects of strontium and silicon on cells of the bone as well as the increasing incidence of osteoporotic fractures, calcium phosphate-based bone cements containing silicon and strontium might represent a promising tool for bone replacement therapies of systemically altered bone. However, information about combined effects of strontium and silicon on osteoclastogenesis is still not available. Therefore, differentiation capacity of human peripheral blood mononuclear cells (hPBMCs) into osteoclast-like cells was investigated by culturing the cells in combination with a strontium- (pS100) and a strontium/silicon- modified calcium phosphate bone cement (pS100-G)...
December 7, 2018: Biomedical Materials
Rachel Elizabeth Wilbur, Jacob Spencer Griffin, Mark Sorensen, Robert Daniel Furberg
BACKGROUND: Commercial salmon fishing in Alaska is one of the most dangerous occupations in the United States. Between 1992 and 2008, the average annual industry mortality rate was 128 deaths per 100,000 workers, and despite an increase in industry regulations, there has not been a significant decrease in mortality rate since 2000. Unpredictable fishing openings and fierce competition for limited resources result in periods of intense sleep deprivation and physical strain during the short commercial salmon season in Alaska...
December 10, 2018: JMIR Research Protocols
Chris R Marcellino, Samuel Lapalme-Remis, Alejandro A Rabinstein, Jamie J Van Gompel, Gregory A Worrell, Eric T Payne, Sara Hocker
Seizures and other electrophysiological disturbances are an under-recognized cause of coma, focal deficits, and prolonged encephalopathy following subdural hematoma evacuation. In these patients, it is possible that seizures remain unrecognized on scalp EEG. It has been shown that a high burden of seizures and other electrophysiological disturbances exist following surgical evacuation and underlie the encephalopathy commonly seen in this patient population, predisposing them to medical complications and confounding estimates of prognosis...
December 10, 2018: Epileptic Disorders: International Epilepsy Journal with Videotape
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