Jie Liu, Paul F Garcia Bardales, Kamrul Islam, Sheikh Jarju, Jane Juma, Chimwemwe Mhango, Queen Naumanga, Sonia Qureshi, Catherine Sonye, Naveed Ahmed, Fatima Aziz, Md Taufiqur Rahman Bhuiyan, Mary Charles, Nigel A Cunliffe, Mahamadou Abdou, Sean R Galagan, Ensa Gitteh, Ibrehima Guindo, M Jahangir Hossain, Abdoulie M J Jabang, Khuzwayo C Jere, Flywell Kawonga, Mariama Keita, Noumou Yakhouba Keita, Karen L Kotloff, Wagner V Shapiama Lopez, Stephen Munga, Maribel Paredes Olortegui, Richard Omore, Patricia B Pavlinac, Firdausi Qadri, Farah Naz Qamar, S M Azadul Alam Raz, Laura Riziki, Francesca Schiaffino, Suzanne Stroup, Sarata Nassoun Traore, Tackeshy Pinedo Vasquez, Mohammad Tahir Yousafzai, Martin Antonio, Jennifer E Cornick, Furqan Kabir, Farhana Khanam, Margaret N Kosek, John Benjamin Ochieng, James A Platts-Mills, Sharon M Tennant, Eric R Houpt
BACKGROUND: Quantitative polymerase chain reaction (qPCR) targeting ipaH has been proven to be highly efficient in detecting Shigella in clinical samples compared to culture-based methods, which underestimate Shigella burden by 2- to 3-fold. qPCR assays have also been developed for Shigella speciation and serotyping, which is critical for both vaccine development and evaluation. METHODS: The Enterics for Global Health (EFGH) Shigella surveillance study will utilize a customized real-time PCR-based TaqMan Array Card (TAC) interrogating 82 targets, for the detection and differentiation of Shigella spp, Shigella sonnei , Shigella flexneri serotypes, other diarrhea-associated enteropathogens, and antimicrobial resistance (AMR) genes...
March 2024: Open Forum Infectious Diseases