keyword
https://read.qxmd.com/read/38533131/progerin-inhibits-the-proliferation-and-migration-of-melanoma-cells-by-regulating-the-expression-of-paxillin
#21
JOURNAL ARTICLE
Weixian Liu, Xinxian Huang, Weizhao Luo, Xinguang Liu, Weichun Chen
OBJECTIVE: Progerin, the underlying cause of Hutchinson-Gilford Progeria Syndrome (HGPS), has been extensively studied for its impact on normal cells and premature aging patients. However, there is a lack of research on its specific effects on tumor cells. Melanoma is one of the most common malignant tumors with high morbidity and mortality. This study aimed to elucidate the potential therapeutic role of progerin in melanoma. MATERIALS AND METHODS: We constructed the melanoma A375 cell line and M14 cell line with stable expression of progerin...
2024: OncoTargets and Therapy
https://read.qxmd.com/read/38533068/prognostic-prediction-using-a-gene-signature-developed-based-on-exhausted-t-cells-for-liver-cancer-patients
#22
JOURNAL ARTICLE
Yu Zhou, Wanrui Wu, Wei Cai, Dong Zhang, Weiwei Zhang, Yunling Luo, Fujing Cai, Zhenjing Shi
BACKGROUND: Liver hepatocellular carcinoma (LIHC) is a solid primary malignancy with poor prognosis. This study discovered key prognostic genes based on T cell exhaustion and used them to develop a prognostic prediction model for LIHC. METHODS: SingleR's annotations combined with Seurat was used to automatically annotate the single-cell clustering results of the LIHC dataset GSE166635 downloaded from the Gene Expression Omnibus (GEO) database and to identify clusters related to exhausted T cells...
March 30, 2024: Heliyon
https://read.qxmd.com/read/38533032/metal-ion-stimulation-related-gene-signatures-correlate-with-clinical-and-immunologic-characteristics-of-glioma
#23
JOURNAL ARTICLE
Chengzhi Jiang, Binbin Zhang, Wenjuan Jiang, Pengtao Liu, Yujia Kong, Jianhua Zhang, Wenjie Teng
BACKGROUND: Environmental factors serve as one of the important pathogenic factors for gliomas. Yet people focus only on the effect of electromagnetic radiation on its pathogenicity, while metals in the environment are neglected. This study aimed to investigate the relationship between metal ion stimulation and the clinical characteristics and immune status of GM patients. METHODS: Firstly, mRNA expression profiles of GM patients and normal subjects were obtained from Chinese GM Genome Atlas (CGGA) and Gene Expression Omnibus (GEO) to identify differentially expressed metal ion stimulation-related genes(DEMISGs)...
March 30, 2024: Heliyon
https://read.qxmd.com/read/38533005/prognostic-correlation-between-specialized-capillary-endothelial-cells-and-lung-adenocarcinoma
#24
JOURNAL ARTICLE
Rongchang Zhao, Yan Ding, Rongbo Han, Rongjie Ding, Jun Liu, Chunrong Zhu, Dan Ding, Minhui Bao
BACKGROUND: In-depth analysis of the functional changes occurring in endothelial cells (ECs) involved in capillary formation can help to elucidate the mechanism of tumour vascular growth. METHODS: Appropriate datasets were retrieved from the GEO database to obtain single-cell data on LUAD samples and adjacent normal tissue samples. ECs were selected by an automatic annotation program in R and further subdivided based on reported EC marker genes. Functional changes in different types of capillary ECs were then visualized, and the concrete expression was classified by genetic data in the TCGA...
March 30, 2024: Heliyon
https://read.qxmd.com/read/38532933/novel-biomarkers-of-inflammation-associated-immunity-in-cervical-cancer
#25
JOURNAL ARTICLE
Weihong Zhao, Qi Li, Songquan Wen, Yaqin Li, Ying Bai, Zhiyu Tian
BACKGROUND: Cervical cancer (CC) is a highly malignant gynecological cancer with a direct causal link to inflammation, primarily resulting from persistent high-risk human papillomavirus (HPV) infection. Given the challenges in early detection and mid to late-stage treatment, our research aims to identify inflammation-associated immune biomarkers in CC. METHODS: Using a bioinformatics approach combined with experimental validation, we integrated two CC datasets (GSE39001 and GSE63514) in the Gene Expression Omnibus (GEO) to eliminate batch effects...
2024: Frontiers in Oncology
https://read.qxmd.com/read/38532632/m6a-mediated-molecular-patterns-and-tumor-microenvironment-infiltration-characterization-in-nasopharyngeal-carcinoma
#26
JOURNAL ARTICLE
Yong Wang, Lisha Peng, Feng Wang
N6-methyladenosine (m6A) is the most predominant RNA epigenetic regulation in eukaryotic cells. Numerous evidence revealed that m6A modification exerts a crucial role in the regulation of tumor microenvironment (TME) cell infiltration in several tumors. Nevertheless, the potential role and mechanism of m6A modification in nasopharyngeal carcinoma (NPC) remains unknown. mRNA expression data and clinical information from GSE102349, and GSE53819 datasets obtained from Gene Expression Omnibus (GEO) was used for differential gene expression and subsequent analysis...
December 31, 2024: Cancer Biology & Therapy
https://read.qxmd.com/read/38532629/need-for-standardization-of-cytokine-profiling-in-car-t-cell-therapy
#27
JOURNAL ARTICLE
D Nathan Biery, David P Turicek, Caroline Diorio, Brett A Schroeder, Nirali N Shah
With expansion of CAR T-cell therapy and broader utilization of anti-cytokine directed therapeutics for toxicity mitigation, the routine assessment of cytokines may enhance understanding of toxicity profiles, guide therapeutic interventions, and facilitate cross-trial comparisons. As specific cytokine elevations can correlate with and provide insights into CAR T-cell toxicity, mitigation strategies, and response, we explored the reporting of cytokine detection methods and assessed for the correlation of cytokines to CRS and ICANS across clinical trials...
March 25, 2024: Molecular Therapy
https://read.qxmd.com/read/38532606/rgie-a-gene-selection-method-related-to-radiotherapy-resistance-in-head-and-neck-squamous-cell-carcinoma
#28
JOURNAL ARTICLE
Qingzhe Meng, Dunhui Liu, Junhong Huang, Xinjie Yang, Huan Li, Zihui Yang, Jun Wang, Wanpeng Gao, Yahui Li, Rong Liu, Liying Yang, Jianhua Wei
BACKGROUND: Head and Neck Squamous Cell Carcinoma (HNSCC) is a malignant tumor with a high degree of malignancy, invasiveness, and metastasis rate. Radiotherapy, as an important adjuvant therapy for HNSCC, can reduce the postoperative recurrence rate and improve the survival rate. Identifying the genes related to HNSCC radiotherapy resistance (HNSCC-RR) is helpful in the search for potential therapeutic targets. However, identifying radiotherapy resistance-related genes from tens of thousands of genes is a challenging task...
March 26, 2024: Current Radiopharmaceuticals
https://read.qxmd.com/read/38532411/a-solution-for-highly-efficient-electroporation-of-primary-cytotoxic-t-lymphocytes
#29
JOURNAL ARTICLE
Nadia Alawar, Claudia Schirra, Meltem Hohmann, Ute Becherer
BACKGROUND: Cytotoxic T lymphocytes (CTLs) are central players in the adaptive immune response. Their functional characterization and clinical research depend on efficient and reliable transfection. Although various methods have been utilized, electroporation remains the preferred technique for transient gene over-expression. However, the efficiency of electroporation is reduced for human and mouse primary CTLs. Lonza offers kits that effectively improve plasmid DNA transfection quality...
March 26, 2024: BMC Biotechnology
https://read.qxmd.com/read/38532369/preliminary-findings-on-the-development-of-a-predictive-model-for-blca-based-on-disulfidptosis-associated-incrnas-signature
#30
JOURNAL ARTICLE
Chao Tang, Yanpeng Fan, Shusheng Zhu
BACKGROUND: Bladder urothelial carcinoma (BLCA) is the most common malignancy of the urinary tract, presenting with a wide range of clinical symptoms and prognosis. Disulfidptosis is a newly identified cell death method and closely associated with BLCA progression, prognosis, and treatment outcome. Currently, we need to construct a new prognostic model for disulfidptosis-related long noncoding RNAs (drlncRNAs) to improve the treatment strategy of BLCA. METHODS: The data for BLCA samples were obtained from The Cancer Genome Atlas (TCGA), and then 10 unique genes related to disulfidoptosis (DRGs) were identified from research papers...
March 26, 2024: BMC Urology
https://read.qxmd.com/read/38532320/pungency-related-gene-network-in-allium-sativum-l-response-to-sulfur-treatments
#31
JOURNAL ARTICLE
Ali Ammarellou
Pungency of garlic (Allium sativum L.) is generated from breakdown of the alk(en)yl cysteine sulphoxide (CSO), alliin and its subsequent breakdown to allicin under the activity of alliinase (All). Based on recent evidence, two other important genes including Sulfite reductase (SiR) and Superoxide dismutase (SOD) are thought to be related to sulfur metabolism. These three gene functions are in sulfate assimilation pathway. However, whether it is involved in stress response in crops is largely unknown. In this research, the order and priority of simultaneous expression of three genes including All, SiR and SOD were measured on some garlic ecotypes of Iran, collected from Zanjan, Hamedan and Gilan, provinces under sulfur concentrations (0, 6, 12, 24 and 60 g/ per experimental unit: pot) using real-time quantitative PCR (RT-qPCR) analysis...
March 26, 2024: BMC genomic data
https://read.qxmd.com/read/38532207/development-of-a-screening-system-of-gene-sets-for-estimating-the-time-of-early-skeletal-muscle-injury-based-on-second-generation-sequencing-technology
#32
JOURNAL ARTICLE
Junyi Shen, Hao Sun, Shidong Zhou, Liangliang Wang, Chaoxiu Dong, Kang Ren, Qiuxiang Du, Jie Cao, Yingyuan Wang, Junhong Sun
The present study is aimed to address the challenge of wound age estimation in forensic science by identifying reliable genetic markers using low-cost and high-precision second-generation sequencing technology. A total of 54 Sprague-Dawley rats were randomly assigned to a control group or injury groups, with injury groups being further divided into time points (4 h, 8 h, 12 h, 16 h, 20 h, 24 h, 28 h, and 32 h after injury, n = 6) to establish rat skeletal muscle contusion models...
March 27, 2024: International Journal of Legal Medicine
https://read.qxmd.com/read/38531930/correlation-analysis-of-disulfidptosis-related-gene-signatures-with-clinical-prognosis-and-immunotherapy-response-in-sarcoma
#33
JOURNAL ARTICLE
Juan Xu, Kangwen Guo, Xiaoan Sheng, Yuting Huang, Xuewei Wang, Juanjuan Dong, Haotian Qin, Chao Wang
Disulfidptosis, a newly discovered type of programmed cell death, could be a mechanism of cell death controlled by SLC7A11. This could be closely associated with tumor development and advancement. Nevertheless, the biological mechanism behind disulfidptosis-related genes (DRGs) in sarcoma (SARC) is uncertain. This study identified three valuable genes (SLC7A11, RPN1, GYS1) associated with disulfidptosis in sarcoma (SARC) and developed a prognostic model. The multiple databases and RT-qPCR data confirmed the upregulated expression of prognostic DRGs in SARC...
March 26, 2024: Scientific Reports
https://read.qxmd.com/read/38531918/the-complete-mitochondrial-genome-of-leucoptera-coffeella-lepidoptera-lyonetiidae-and-phylogenetic-relationships-within-the-yponomeutoidea-superfamily
#34
JOURNAL ARTICLE
Mateus Pereira Dos Santos, Ana Paula Zotta Mota, Roberto Coiti Togawa, Natalia Florencio Martins, Eliza Fabricio de Melo Bellard do Nascimento, Vivian Santos Lucena, Maria Aparecida Castellani, Erika Valéria Saliba Albuquerque, Frédérique Hilliou
The coffee leaf miner (Leucoptera coffeella) is one of the major pests of coffee crops in the neotropical regions, and causes major economic losses. Few molecular data are available to identify this pest and advances in the knowledge of the genome of L. coffeella will contribute to improving pest identification and also clarify taxonomy of this microlepidoptera. L. coffeella DNA was extracted and sequenced using PacBio HiFi technology. Here we report the complete L. coffeella circular mitochondrial genome (16,407 bp) assembled using Aladin software...
March 26, 2024: Scientific Reports
https://read.qxmd.com/read/38531678/a-cytological-f1-rnai-screen-for-defects-in-drosophila-melanogaster-female-meiosis
#35
JOURNAL ARTICLE
William D Gilliland, Dennis P May, Amelia O Bowen, Kelly O Conger, Doreen Elrad, Marcin Marciniak, Sarah A Mashburn, Gabrielle Presbitero, Lucas F Welk
Genetic screens for recessive alleles induce mutations, make the mutated chromosomes homozygous, and then assay those homozygotes for the phenotype of interest. When screening for genes required for female meiosis, the phenotype of interest has typically been nondisjunction from chromosome segregation errors. As this requires that mutant females be viable and fertile, any mutants that are lethal or sterile when homozygous cannot be recovered by this approach. To overcome these limitations, we have screened the VALIUM22 collection of RNAi constructs that target germline-expressing genes in a vector optimized for germline expression by driving RNAi with GAL4 under control of a germline-specific promoter (nanos or mat-alpha4)...
March 26, 2024: Genetics
https://read.qxmd.com/read/38531630/ezetimibe-inhibits-the-migration-and-invasion-of-triple-negative-breast-cancer-cells-by-targeting-tgf%C3%AE-2-and%C3%A2-emt
#36
JOURNAL ARTICLE
Lingkai Kong, Qinyu He, Ding Ma, Weiwei Shi, Qilei Xin, Chunping Jiang, Junhua Wu
The important role of cholesterol in tumor metastasis has been widely studied in recent years. Ezetimibe is currently the only selective cholesterol uptake inhibitor on the market. Here, we explored the effect of ezetimibe on breast cancer metastasis by studying its impact on breast cancer cell migration, invasion, and epithelial-mesenchymal transition (EMT). Differential gene expression analysis and validation were also carried out to compare ezetimibe-treated and untreated breast cancer cells. Finally, breast cancer cells overexpressing TGFβ2 were constructed, and the effect of TGFβ2 on the migration and invasion of ezetimibe-treated breast cancer cells was examined...
March 26, 2024: FEBS Open Bio
https://read.qxmd.com/read/38531616/signature-reversion-of-three-disease-associated-gene-signatures-prioritizes-cancer-drug-repurposing-candidates
#37
JOURNAL ARTICLE
Jennifer L Fisher, Elizabeth J Wilk, Vishal H Oza, Sam E Gary, Timothy C Howton, Victoria L Flanary, Amanda D Clark, Anita B Hjelmeland, Brittany N Lasseigne
Drug repurposing is promising because approving a drug for a new indication requires fewer resources than approving a new drug. Signature reversion detects drug perturbations most inversely related to the disease-associated gene signature to identify drugs that may reverse that signature. We assessed the performance and biological relevance of three approaches for constructing disease-associated gene signatures (i.e., limma, DESeq2, and MultiPLIER) and prioritized the resulting drug repurposing candidates for four low-survival human cancers...
March 26, 2024: FEBS Open Bio
https://read.qxmd.com/read/38531491/transcriptomic-regulatory-analysis-of-skeletal-muscle-development-in-landrace-pigs
#38
JOURNAL ARTICLE
Mubin Yu, Yanqin Feng, Jiamao Yan, Xiaoyuan Zhang, Zhe Tian, Tao Wang, Junjie Wang, Wei Shen
The development of pig skeletal muscle is a complex dynamic regulation process, which mainly includes the formation of primary and secondary muscle fibers, the remodeling of muscle fibers and the maturation of skeletal muscle; However, the regulatory mechanism of the entire developmental process remains unclear. This study analyzed the whole-transcriptome data of skeletal muscles at 27 developmental nodes (E33-D180) in Landrace pigs, and their key regulatory factors in the development process were identified using the bioinformatics method...
March 24, 2024: Gene
https://read.qxmd.com/read/38530974/conductive-and-enhanced-mechanical-strength-of-mo-2-ti-2-c-3-mxene-based-hydrogel-promotes-neurogenesis-and-bone-regeneration-in-bone-defect-repair
#39
JOURNAL ARTICLE
Hongyu Wang, Yu-Ching Hsu, Chune Wang, Xiao Xiao, Zhengbin Yuan, Yong Zhu, Dazhi Yang
Bone defects are common with increasing high-energy fractures, tumor bone invasion, and implantation revision surgery. Bone is an electroactive tissue that has electromechanical interaction with collogen, osteoblasts, and osteoclasts. Hydrogel provides morphological plasticity and extracellular matrix (ECM) 3D structures for cell survival, and is widely used as a bone engineering material. However, the hydrogels have poor mechanical intensity and lack of cell adhesion, slow gelation time, and limited conductivity...
March 26, 2024: ACS Applied Materials & Interfaces
https://read.qxmd.com/read/38530533/an-experimental-phototherapy-device-for-studying-the-effects-of-blue-light-on-patients-with-raynaud-s-phenomenon
#40
JOURNAL ARTICLE
Brett Levac, James Kerber, Emily Wagner, Jerry A Molitor, Steven S Saliterman
Raynaud's phenomenon (RP) is a condition that causes decreased blood flow to areas perfused by small blood vessels (e.g., fingers, toes). In severe cases, ulceration, gangrene, and loss of fingers may occur. Most treatments focus on inducing vasorelaxation in affected areas by the way of pharmaceuticals. Recently, animal studies have shown that vasorelaxation can be induced by non-coherent blue light (wavelength ~ 430-460 nm) through the actions of melanopsin, a photoreceptive opsin protein encoded by the OPN4 gene...
March 26, 2024: Annals of Biomedical Engineering
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