Carlota Oleaga, Sabine Welten, Audrey Belloc, Anna Solé, Laura Rodriguez, Núria Mencia, Elisabet Selga, Alicia Tapias, Veronique Noé, Carlos J Ciudad
Sp1 is a transcription factor regulating many genes through its DNA binding domain, containing three zinc fingers. We were interested in identifying target genes regulated by Sp1, particularly those involved in proliferation and cancer. Our approach was to treat HeLa cells with a siRNA directed against Sp1 mRNA to decrease the expression of Sp1 and, in turn, the genes activated by this transcription factor. Sp1-siRNA treatment led to a great number of differentially expressed genes as determined by whole genome cDNA microarray analysis...
December 15, 2012: Biochemical Pharmacology