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recombinant allergens

Che Huilian, Yuzhu Zhang, Shu-Chen Lyu, Kari C Nadeau, Tara McHugh
Almond is one of the tree nuts listed by US FDA as a food allergen source. A food allergen identified with patient sera has been debated to be the 2S albumin or the 7S vicilin. However, neither of these proteins has been defined as a food allergen. The purpose of this study was to clone, express, and purify almond vicilin and test whether it is a food allergen. Western blot experiment was performed with 18 individual sera from patients with double-blind, placebo-controlled clinical almond allergy. The results showed that 44% of the sera contained IgE antibodies that recognized the recombinant almond vicilin, indicating that it is an almond allergen...
December 4, 2018: Journal of Agricultural and Food Chemistry
Janice A Layhadi, Ibon Eguiluz-Gracia, Mohamed H Shamji
PURPOSE OF REVIEW: Sublingual allergen immunotherapy (SLIT), a disease-modifying treatment for allergic rhinitis, can induce long-term clinical benefits which are mediated by immune responses that include generation of regulatory B (Breg) and T (Treg) cells. The newest member of the IL-12 superfamily, IL-35, is an anti-inflammatory cytokine known to be produced by Breg and Treg cells. Limited studies are available on the role of IL-35 on allergic rhinitis and during SLIT. This review summarizes recent findings relevant to the topic of IL-35 and their role in SLIT...
November 29, 2018: Current Opinion in Allergy and Clinical Immunology
Bhakkawarat Kulwanich, Sasipa Thanyaratsrisakul, Orathai Jirapongsananuruk, Belinda J Hales, Wayne R Thomas, Surapon Piboonpocanun
PURPOSE: Hypoallergenic recombinant Der p 2 has been produced by various genetic manipulations, but mutation of a naturally polymorphic amino acid residue known to affect IgE binding has not been studied. This study aimed to determine the effect of a point mutation (S47W) of residue 47 of Der p 2 on its structure and immunoglobulin (Ig) E binding. Its ability to induce pro-inflammatory responses and to induce blocking IgG antibody was also determined. METHODS: S47 of recombinant Der p 2...
January 2019: Allergy, Asthma & Immunology Research
Raffaela Campana, Katharina Marth, Petra Zieglmayer, Milena Weber, Christian Lupinek, Yury Zhernov, Olga Elisyutina, Musa Khaitov, Eva Rigler, Kerstin Westritschnig, Uwe Berger, Martin Wolkersdorfer, Fritz Horak, Friedrich Horak, Rudolf Valenta
This is the first double-blind, placebo-controlled study showing that vaccination with recombinant hypoallergenic allergen-derivatives is safe and induces sustained allergen-specific IgG responses which block allergic patients IgE binding to the allergen.
November 21, 2018: Journal of Allergy and Clinical Immunology
Saeideh Tabesh, Songwe Fanuel, Mohammad Reza Fazlollahi, Mir Saeed Yekaninejad, Gholam Ali Kardar, Seyed Alireza Razavi
BACKGROUND: The Salsola kali (S. kali) pollen is one of the most important causes of allergic rhinitis in the deserts and semi-desert areas. Immunotherapy with allergen extracts remains the only available treatment addressing the underlying mechanism of allergy. However, given the low efficacy of this method, it is necessary to find more effective and alternative therapeutic interventions using molecular biology and bioinformatics tools. In this study, a hypoallergenic vaccine was designed on the basis of B-cell epitope approach for S...
November 13, 2018: International Immunopharmacology
Yaroslav V Radzyukevich, Ninel I Kosyakova, Isabella R Prokhorenko
PURPOSE: House dust mites Dermatophagoides pteronyssinus are the main source of major inhalatory allergens inducing inflammatory response. Mite extract contain both allergenic proteins and lipopolysaccharides (LPS). The main allergenic protein, Der p 2, is a functional homolog of sMD-2, a protein providing blood cell response on LPS. Der p 2 may restore the response to LPS in absence of MD-2, but its interaction with LPS in whole blood is unknown. We studied the effect of Der p 2 on LPS-mediated activation of human whole blood cells...
2018: PloS One
O Pfaar, H Lou, Y Zhang, L Klimek, L Zhang
Allergen immunotherapy (AIT) is the only disease-modifying treatment option for patients with IgE-mediated inhalant allergies. Though used in clinical practice for more than 100 years, most innovations in AIT efficacy and safety have been developed in the last two decades. This expert review aimed to highlight the recent progress in AIT for both application routes, the sublingual (SLIT) and subcutaneous (SCIT) forms. As such, it covers recent aspects regarding efficacy and safety in clinical trials and real-life data and outlines new concepts in consensus and position papers as well as in guidelines for AIT...
October 29, 2018: Allergy
Simon Blank
No abstract text is available yet for this article.
October 24, 2018: Allergy
Liyou An, Lan Yang, Yuejin Huang, Yong Cheng, Fuliang Du
Exogenous genes can be site-specifically integrated into the genomic DNA of animals by homologous recombination, generating transgenic animals. These animals have a clear hereditary background, although position effects of the exogenous genes and potential functional disruption of host genes can be caused by the genetic inserts. Therefore, the generation of mammary gland bioreactors via gene-targeting methods is a great asset for producing recombinant proteins in milk. Here, we describe a method of generating gene-targeted goats with the human alpha-lactalbumin gene (hα-LA) integrated into the beta-lactoglobulin gene (BLG) locus...
2019: Methods in Molecular Biology
Ivan Mrkić, Rajna Minić, Dragan Popović, Irena Živković, Marija Gavrović-Jankulović
Aim To investigate the immunomodulatory potential of a chimera composed of the receptor-binding domain of hemagglutinin 1 (H1s) from Influenza virus and Der p 2 (D2) allergen for allergen-specific immunotherapy of house-dust mite allergy (HDM). MAIN METHODS: H1sD2 chimera and D2 allergen were produced by genetic engineering in E. coli. Recombinant antigens were extracted from inclusion bodies by urea, then refolded and purified by immobilized- metal affinity chromatography (IMAC). Purity was verified by 2D-PAGE and secondary structures were assessed by CD spectroscopy...
October 20, 2018: Life Sciences
Ling Li, Jun Qian, Ying Zhou, Yubao Cui
Allergies to various environmental factors, in particular mite species, represent a considerable healthcare burden with lost productivity resulting from symptoms including asthma, conjunctivitis, rhinitis, and atopic dermatitis. The complexity of mite species and the allergens that they produce complicates diagnosis and treatment; however, the advent of recombinant DNA technologies now allows clinicians to better pinpoint the specific sensitizing agents and creates new opportunities for avoidance or immunotherapy...
January 2018: International Journal of Immunopathology and Pharmacology
Pannathee Prangtaworn, Urai Chaisri, Watee Seesuay, Kodchakorn Mahasongkram, Nattawat Onlamoon, Onrapak Reamtong, Anchalee Tungtrongchitr, Nitaya Indrawattana, Wanpen Chaicumpa, Nitat Sookrung
Allergen-specific immunotherapy (AIT) facilitates long-term resolution of allergic morbidity resulting in reduced drug use and increased refractoriness to new sensitization. AIT effectiveness has been demonstrated in seasonal and perennial allergies, and insect stings. However, data and studies in AIT relative to cockroach (CR) allergy are relatively scarce. In this study, mice allergic to American CR (Periplaneta americana) were treated with a liposome (L)-entrapped vaccine made of mouse Tregitope289-Per a 9 of the CR, Tregitope167-Per a 9, or Per a 9 alone - or placebo...
October 19, 2018: Scientific Reports
Rodolfo Bianchini, Franziska Roth-Walter, Anna Ohradanova-Repic, Sabine Flicker, Karin Hufnagl, Michael Bernhard Fischer, Hannes Stockinger, Erika Jensen-Jarolim
BACKGROUND: Macrophages can be converted in vitro into immunoregulatory M2b macrophages in the presence of immune complexes (ICs), but the role of the specific subclasses IgG1 or IgG4 in this phenotypic and functional change is not known. OBJECTIVE: We aimed to refine the original method by applying precisely defined ICs of the subclasses IgG4 or IgG1 constructed by two independent methods. METHODS: Monocyte-derived macrophages (MDMs) were treated with M-CSF, followed by IL-4/IL-13 to induce the M2a allergic phenotype...
October 18, 2018: Allergy
Nikolaos Douladiris, Victoria Garib, Margit Focke Tejkl, Rudolf Valenta, Nikolaos G Papadopoulos, Birgit Linhart
BACKGROUND: Skin testing represents a commonly used first diagnostic method in clinical practice but allergen extracts may vary in composition and often contain cross-reactive allergens and therefore do not always allow the precise identification of the sensitizing allergen source. Our aim was to investigate the suitability of a single recombinant hybrid molecule, consisting of the four major timothy grass pollen allergens (Phl p 1, Phl p 2, Phl p 5, Phl p 6) for in vivo diagnosis of genuine grass pollen allergy in children suffering from pollinosis...
October 14, 2018: Pediatric Allergy and Immunology
Wat Mitthamsiri, Panitan Pradubpongsa, Atik Sangasapaviliya, Tadech Boonpiyathad
PURPOSE: Group 2 innate lymphoid cells (ILC2s) have been implicated in the pathogenesis of allergic disease. However, the effect of allergen-specific immunotherapy (AIT) on ILCs remains to be clarified. The aim of this study was to evaluate the levels of ILC subsets in allergic rhinitis (AR) patients in response to house dust mite (HDM)-specific immunotherapy. METHODS: We enrolled 37 AR patients undergoing AIT (16 responders and 11 non-responders) for 2 years, 35 HDM AR patients and 28 healthy subjects...
November 2018: Allergy, Asthma & Immunology Research
Rudolf Valenta, Alexander Karaulov, Verena Niederberger, Yury Zhernov, Olga Elisyutina, Raffaela Campana, Margarete Focke-Tejkl, Mirela Curin, Leyla Namazova-Baranova, Jiu-Yao Wang, Ruby Pawankar, Musa Khaitov
Today, in vivo allergy diagnosis and allergen-specific immunotherapy (AIT) are still based on allergen extracts obtained from natural allergen sources. Several studies analyzing the composition of natural allergen extracts have shown severe problems regarding their quality such as the presence of undefined nonallergenic materials, contaminants as well as high variabilities regarding contents and biological activity of individual allergens. Despite the increasing availability of sophisticated analytical technologies, these problems cannot be overcome because they are inherent to allergen sources and methods of extract production...
November 2018: Journal of Allergy and Clinical Immunology in Practice
S Peveri, S Pattini, M T Costantino, C Incorvaia, M Montagni, C Roncallo, D Villalta, E Savi
BACKGROUND: Component resolved diagnosis (CRD) allows to precisely identify the sensitization to specific molecules of a given allergenic source, resulting in an important improvement in clinical management, particularly of polysensitized subjects. This will end in the correct prescription of allergen immunotherapy (AIT) for respiratory allergy and in adequate avoidance diets or prescription of self-injectable adrenaline in food allergy. OBJECTIVE: The aim of this multicenter, real life study is to evaluate the percentage change of the diagnostic-therapeutic choice in polysensitized patients with respiratory allergy and in patients with food allergy, after using CRD compared to a first level diagnosis, along with an economic analysis of the patient's overall management according to the two different approaches...
September 20, 2018: Allergologia et Immunopathologia
P J Sarate, S Heinl, S Poiret, M Drinić, C Zwicker, I Schabussova, C Daniel, U Wiedermann
Allergic poly-sensitization affects a large number of allergic patients and poses a great challenge for their treatment. In this study we evaluated the effects of the probiotic Escherichia coli Nissle 1917 (EcN) expressing a birch and grass pollen allergen chimera 'Bet v 1, Phl p 1 and Phl p 5' (EcN-Chim) on allergy prevention after oral or intranasal application in poly-sensitized mice. In contrast to oral application, intranasal pretreatment with EcN-Chim prior to poly-sensitization led to a significant reduction of lung inflammation (eosinophils, IL-5, and IL-13 in bronchoalveolar lavage) along with suppressed levels of allergen-specific serum IgE...
September 21, 2018: Mucosal Immunology
Pinya Pulsawat, Tewarit Soongrung, Pattraporn Satitsuksanoa, Maxime Le Mignon, Souad Khemili, Dimitri Gilis, Emmanuel Nony, Malcolm W Kennedy, Alain Jacquet
BACKGROUND: Protein crystallographic studies suggest that the house dust mite (HDM) allergen Der p 5 potentially interacts with hydrophobic ligands. Der p 5, in association with its ligand(s), might therefore trigger innate immune signalling pathways in the airway epithelium and influence the initiation of the HDM-allergic response. OBJECTIVE: We investigated the lipid binding propensities of recombinant (r)Der p 5 and characterized the signalling pathways triggered by the allergen in airway epithelial cells...
September 19, 2018: Clinical and Experimental Allergy: Journal of the British Society for Allergy and Clinical Immunology
Yanhui Wen, Lifeng Zhou, Yuqiu Li, Zhengmin Li, Weijun Deng, Tao Zhang
OBJECTIVE: Increasing evidence suggests that leptin is upregulated during allergic reactions in the airway and related to the severity of disease in allergic rhinitis (AR). In this study, we aimed to investigate the expression of leptin during sublingual immunotherapy (SLIT) in AR patients. METHODS: Forty AR patients without obesity were recruited in this study. Twenty patients received house dust mite (HDM) allergen extract for SLIT and twenty patients received placebo randomly...
September 14, 2018: European Archives of Oto-rhino-laryngology
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