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recombinant allergens

Tristan Hayes, Amanda Rumore, Brad Howard, Xin He, Mengyao Luo, Sabina Wuenschmann, Martin Chapman, Shiv Kale, Liwu Li, Hirohito Kita, Christopher B Lawrence
Allergens are molecules that elicit a hypersensitive inflammatory response in sensitized individuals and are derived from a variety of sources. Alt a 1 is the most clinically important secreted allergen of the ubiquitous fungus, Alternaria . It has been shown to be a major allergen causing IgE-mediated allergic response in the vast majority of Alternaria -sensitized individuals. However, no studies have been conducted in regards to the innate immune eliciting activities of this clinically relevant protein. In this study, recombinant Alt a 1 was produced, purified, labeled, and incubated with BEAS-2B, NHBE, and DHBE human lung epithelial cells...
2018: Frontiers in Immunology
Arthur H Totten, Li Xiao, Danlin Luo, David Briles, Joanetha Y Hale, Donna M Crabb, Trenton R Schoeb, Ammar Saadoon Alishlash, Ken B Waites, T Prescott Atkinson
BACKGROUND: Mycoplasma pneumoniae (Mpn), an atypical human pathogen, has been associated with asthma initiation and exacerbation. Asthmatics have been reported to have higher carriage rates of Mpn compared to non-asthmatics, and are at greater risk for invasive respiratory infections. OBJECTIVE: To study whether prior allergen sensitization affects the host response to chronic bacterial infection. METHODS: BALB/cJ and IL-4Rα-/- mice were sensitized with ovalbumin (OVA), and then infected with Mpn or Streptococcus pneumoniae (Spn)...
August 6, 2018: Journal of Allergy and Clinical Immunology
Mey-Fann Lee, Chu-Hui Chiang, Ying-Lan Li, Nancy M Wang, Pei-Pong Song, Shyh-Jye Lin, Yi-Hsing Chen
BACKGROUND: American cockroaches (Periplaneta americana) are an important indoor allergen source and a major risk factor for exacerbations and poor control of asthma. We previously reported that allergen components from American cockroaches exhibit varying levels of pathogenicity. Sensitization to major American cockroach allergen, Per a 2, correlated with more severe clinical phenotypes among patients with allergic airway diseases. MATERIALS AND METHODS: In this study, we examined whether oral plant vaccine-encoding full-length Per a 2 clone-996 or its hypoallergenic clone-372 could exert a prophylactic role in Per a 2-sensitized mice...
2018: PloS One
Kashinath Bhattacharya, Gaurab Sircar, Angira Dasgupta, Swati Gupta Bhattacharya
The growing prevalence of allergy and asthma in India has become a major health concern with symptoms ranging from mild rhinitis to severe asthma and even life-threatening anaphylaxis. The "allergen repertoire" of this subcontinent is highly diverse due to the varied climate, flora, and food habits. The proper identification, purification, and molecular characterization of allergy-eliciting molecules are essential in order to facilitate an accurate diagnosis and to design immunotherapeutic vaccines...
July 27, 2018: International Archives of Allergy and Immunology
Pawel Dubiela, Stefan Kabasser, Nicolas Smargiasso, Sabine Geiselhart, Merima Bublin, Christine Hafner, Gabriel Mazzucchelli, Karin Hoffmann-Sommergruber
Walnuts are ranked high in the list of the culprit foods inducing severe allergic reactions. Jug r 2 has been identified as a major allergen in common walnut by cDNA cloning from a somatic cell line. So far, studies were performed on the allergenic activity of recombinant Jug r 2, yet there is still no evidence about the physicochemical characteristics of the natural allergen. Therefore, we aimed to purify and deeply characterize natural Jug r 2 and to assess IgE cross-reactivity among vicilins from different tree nuts...
July 27, 2018: Scientific Reports
Johanna Stoevesandt, Christine Hosp, Andreas Kerstan, Axel Trautmann
BACKGROUND: The prevalence and predisposing factors of asymptomatic sensitization to Hymenoptera venom marker allergens are largely unknown. OBJECTIVE: To evaluate sensitization profiles in a group of 490 dermatologic patients without a history of sting-induced anaphylaxis. METHODS: Clinical data were collected using a structured questionnaire; sera were tested for total IgE and specific IgE to venom preparations, recombinant venom marker allergens, inhalative allergens, and cross-reactive carbohydrate determinants...
July 25, 2018: Clinical and Experimental Allergy: Journal of the British Society for Allergy and Clinical Immunology
Tahere Shahriari-Farfani, Azar Shahpiri, Asghar Taheri-Kafrani
β-Lactoglobulin (BLG), a member of lipocalin family, is one of the major bovine milk allergens. This protein exists as a dimer of two identical subunits and contains two intramolecular disulfide bonds that are responsible for its resistance to trypsin digestion and allergenicity. This study aimed to evaluate the effect of reduction of disulfide bonds of BLG with different rice thioredoxins (Trxs) on its digestibility and allergenicity. Therefore, the active recombinant forms of three rice Trx isoforms (OsTrx1, OsTrx20, and OsTrx23) and one rice NADPH-dependent Trx reductase isoform (OsNTRB) were expressed in Escherichia coli...
July 21, 2018: Applied Biochemistry and Biotechnology
Natasha C Gunawardana, Stephen R Durham
OBJECTIVE: New insights into mechanisms should enable strategic improvement of allergen immunotherapy, aiming to make it safer, faster, more effective and able to induce long-term tolerance. We review novel approaches with potential to translate into clinical use. DATA SOURCES: Database searches were conducted in Pubmed, Scopus and Google Scholar. STUDY SELECTIONS: Search terms were based on current and novel approaches in immunotherapy. Literature was selected primarily from recent randomised double-blind placebo-controlled trials and meta-analyses...
July 16, 2018: Annals of Allergy, Asthma & Immunology
Genoveva Del-Río Camacho, Ana María Montes Arjona, José Fernández-Cantalejo Padial, Rodríguez Catalán Jesús
INTRODUCTION: Specific immunotherapy (SIT) is used to treat asthma and allergic rhinitis, and a dose-response relationship has been found for SIT efficacy, creating a need to accurately select the allergen used in therapy. This need is especially pronounced in poly-sensitized children living in areas where different pollen allergen sources coexist in the same season, as this circumstance complicates diagnostic efforts. In such cases, component-resolved diagnosis (CRD) can increase diagnostic accuracy and aid in SIT prescription...
July 12, 2018: Allergologia et Immunopathologia
Ayumi Fukuoka, Kazufumi Matsushita, Taiyo Morikawa, Takumi Adachi, Koubun Yasuda, Hiroshi Kiyonari, Shigeharu Fujieda, Tomohiro Yoshimoto
BACKGROUND: Protease allergens disrupt epithelial barriers to exert their allergenicity. Cystatin SN (encoded by CST1) is an endogenous cysteine protease inhibitor upregulated in nasal epithelia in patients with allergic rhinitis (AR). OBJECTIVE: To investigate the protective effect of human Cystatin SN on AR symptoms using pollen-induced AR mouse models. METHODS: We performed in vitro protease activity assay to evaluate the effect of recombinant human Cystatin SN (rhCystatin SN) on Japanese Cedar (JC) or ragweed proteases...
July 13, 2018: Journal of Allergy and Clinical Immunology
Andreas M Farkas, Ulrike Baranyi, Georg A Böhmig, Lukas Unger, Stefan Hopf, Markus Wahrmann, Heinz Regele, Benedikt Mahr, Christoph Schwarz, Karin Hock, Nina Pilat, Ivan Kristo, Jasmin Mraz, Christian Lupinek, Josef Thalhamer, Gregor Bond, Lorenz Kuessel, Elizabeth Wlodek, Jack Martin, Menna Clatworthy, Gavin Pettigrew, Rudolf Valenta, Thomas Wekerle
BACKGROUND: Donor-specific antibodies (DSA) of the IgG isotype are routinely measured for diagnostic purposes in renal transplant recipients and are associated with antibody-mediated rejection and long-term graft loss. OBJECTIVE: This study aimed to investigate if MHC-specific antibodies of the IgE isotype are induced during the rejection of allografts. METHODS: Anti-MHC/HLA IgE was measured in sera of mice grafted with skin or heart transplants from various donor strains and in sera of kidney transplant patients with high levels of HLA-IgG...
July 12, 2018: Journal of Allergy and Clinical Immunology
Linmei Li, Shan Wang, Zehong Zou, Ailin Tao, Yuncan Ai
BACKGROUND: Mal f 1, the first allergen cloned from Malassezia furfur, has positive IgE reactivity in sera from atopic dermatitis (AD) patients. The mechanism by which Mal f 1 induces the maturation of human dendritic cells (DCs) and maintains the symptoms of AD is not well understood. OBJECTIVE: The present study aims to explore the activation profile of THP-1 derived dendritic cells (TDDCs) stimulated by recombinant Mal f 1, as well as to explore the IgE-binding ability of rMal f 1 and its correlation with IgE-binding activity of complete allergens of M...
July 10, 2018: International Immunopharmacology
Elisabeth Kurze, Vanessa Kock, Roberto Lo Scalzo, Klaus Olbricht, Wilfried Schwab
Birch pollen allergic patients show cross-reactivity to vegetables and fruits, including strawberries ( Fragaria × ananassa ). The objective of this study was to quantify the level of the Fra a 1 protein, a Bet v 1-homologous protein in strawberry fruits by a newly developed ELISA, and determine the effect of genotype, cultivation and food processing on the allergen amount. An indirect competitive ELISA using a specific polyclonal anti-Fra a 1.02 antibody was established and revealed high variability in Fra a 1 levels within 20 different genotypes ranging from 0...
July 2, 2018: Nutrients
J F Cantillo, L Puerta, E Fernandez-Caldas, J L Subiza, I Soria, S Wöhrl, C Ebner, W Keller, Y Resch-Marat, S Vrtala, B Bohle
BACKGROUND: Aedes aegypti and Dermatophagoides pteronyssinus contain important allergens including cross-reactive tropomyosins. However, the functional and clinical relevance of their cross-reactivity is still debated. OBJECTIVE: To analyse the humoral and cellular cross-reactivity of recombinant Aed a 10.01, Aed a 10.02, and Der p 10. METHODS: Sera from 15 Austrian house dust mite-allergic, Der p 10-sensitized individuals were tested for IgE-reactivity to recombinant tropomyosins in ELISA, inhibition ELISA and basophil activation tests...
July 10, 2018: Clinical and Experimental Allergy: Journal of the British Society for Allergy and Clinical Immunology
Nobuyuki Maruyama, Sakura Sato, Cerrone Cabanos, Akira Tanaka, Komei Ito, Motohiro Ebisawa
BACKGROUND: Soybean is a major food allergen in children. Component-resolved diagnostics has improved the accuracy of diagnosing immunoglobulin E (IgE)-mediated food allergies. OBJECTIVE: We aimed to develop a novel component for the diagnosis of soybean allergy using recombinant technology. METHODS: Japanese paediatric patients with suspected soybean allergy (n = 91) were included, and symptomatic (n = 40) and asymptomatic (n = 51) cases were divided through oral food challenge testing...
July 10, 2018: Clinical and Experimental Allergy: Journal of the British Society for Allergy and Clinical Immunology
Wang Guang-Li, Liang Wei, Li Ling-Yun, Wu Song-Quan
OBJECTIVE: To clone, express, purify the Per a 4 gene encoding an allergen of Periplaneta Americana and prepare monoclonal antibodies against the recombinant allergen. METHODS: The total RNA was extracted from P. Americana , and the target gene was amplified by RT-PCR and cloned into pMD18-T vector. After being confirmed by nucleotide sequencing, the gene was then inserted into pGEX-3X to construct the express vector pGEX-3X-Per a 4. Further, the pGEX-3X-Per a 4 was transformed into E...
May 2018: Annals of Clinical and Laboratory Science
Moumita B Sarkar, Gaurab Sircar, Nandini Ghosh, Abhishek K Das, Kuladip Jana, Angira Dasgupta, Swati G Bhattacharya
Papaya has been reported to elicit IgE-mediated hypersensitivity via pollen inhalation and fruit consumption. Certain papaya sensitive patients with food allergy were found to experience recurrent respiratory distresses even after quitting the consumption of fruits. This observation prompted us to investigate the allergens commonly present in fruits and pollen grains of papaya. A discovery approach consisting of immunoproteomic detection followed by molecular characterization led to the identification of a novel papaya allergen designated as Cari p 1...
2018: Frontiers in Plant Science
Claudia Asam, Anargyros Roulias, Maria A Parigiani, Astrid Haab, Michael Wallner, Martin Wolf, Peter Briza, Barbara Bohle, Fatima Ferreira, Michael Hauser
BACKGROUND: Enhancing the quality and yield of protein production in heterologous expression systems is an important issue for developing new biopharmaceuticals. It has been shown that the dynamics of protein folding is influenced by codon frequencies. As codon usage frequencies are species specific, this can affect heterologous protein expression. In this respect, "codon harmonization," that is, the usage of synonymous codons with usage frequencies in the host resembling the usage frequencies in the native organism, is a promising strategy...
June 25, 2018: International Archives of Allergy and Immunology
Pia Gattinger, Christian Lupinek, Lampros Kalogiros, Mira Silar, Mihaela Zidarn, Peter Korosec, Christine Koessler, Natalija Novak, Rudolf Valenta, Irene Mittermann
BACKGROUND: Allergy to bee and wasp venom can lead to life-threatening systemic reactions. The identification of the culprit species is important for allergen-specific immunotherapy. OBJECTIVES: To determine a panel of recombinant bee and wasp allergens which is suitable for the identification of bee or wasp as culprit allergen sources and to search for molecular surrogates of clinical severity of sting reactions. METHODS: Sera from eighty-seven patients with a detailed documentation of their severity of sting reaction (Mueller grade) and who had been subjected to titrated skin testing with bee and wasp venom were analyzed for bee and wasp-specific IgE levels by ImmunoCAPTM...
2018: PloS One
Alireza Salimi Chirani, Mona Ghazi, Mehdi Goudarzi, Shahin Najar Peerayeh, Hoorieh Soleimanjahi, Masoud Dadashi, Bahareh Hajikhani
Helicobacter pylori (H. pylori) as microaerophilic, Gram-negative bacterium colonize the human gastric milieu, where it impetuses chronic disorders. Vaccination is a complementary plan, along with antibiotic therapy, for clearance of H. pylori. Today, Computer based tools are essential for the evaluation, design, and experiment for novel chimeric targets for immunological administration. The purpose of this experiment was immunoinformatic analysis of UreB and HpaA molecules in a fusion arrangement and also, construction and expression of recombinant protein containing chimeric sequences...
May 5, 2018: Computational Biology and Chemistry
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