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recombinant allergens

Nikolaos Douladiris, Victoria Garib, Margit Focke Tejkl, Rudolf Valenta, Nikolaos G Papadopoulos, Birgit Linhart
BACKGROUND: Skin testing represents a commonly used first diagnostic method in clinical practice but allergen extracts may vary in composition and often contain cross-reactive allergens and therefore do not always allow the precise identification of the sensitizing allergen source. Our aim was to investigate the suitability of a single recombinant hybrid molecule, consisting of the four major timothy grass pollen allergens (Phl p 1, Phl p 2, Phl p 5, Phl p 6) for in vivo diagnosis of genuine grass pollen allergy in children suffering from pollinosis...
October 14, 2018: Pediatric Allergy and Immunology
Wat Mitthamsiri, Panitan Pradubpongsa, Atik Sangasapaviliya, Tadech Boonpiyathad
PURPOSE: Group 2 innate lymphoid cells (ILC2s) have been implicated in the pathogenesis of allergic disease. However, the effect of allergen-specific immunotherapy (AIT) on ILCs remains to be clarified. The aim of this study was to evaluate the levels of ILC subsets in allergic rhinitis (AR) patients in response to house dust mite (HDM)-specific immunotherapy. METHODS: We enrolled 37 AR patients undergoing AIT (16 responders and 11 non-responders) for 2 years, 35 HDM AR patients and 28 healthy subjects...
November 2018: Allergy, Asthma & Immunology Research
Rudolf Valenta, Alexander Karaulov, Verena Niederberger, Yury Zhernov, Olga Elisyutina, Raffaela Campana, Margarete Focke-Tejkl, Mirela Curin, Leyla Namazova-Baranova, Jiu-Yao Wang, Ruby Pawankar, Musa Khaitov
Today, in vivo allergy diagnosis and allergen-specific immunotherapy (AIT) are still based on allergen extracts obtained from natural allergen sources. Several studies analyzing the composition of natural allergen extracts have shown severe problems regarding their quality such as the presence of undefined nonallergenic materials, contaminants as well as high variabilities regarding contents and biological activity of individual allergens. Despite the increasing availability of sophisticated analytical technologies, these problems cannot be overcome because they are inherent to allergen sources and methods of extract production...
October 5, 2018: Journal of Allergy and Clinical Immunology in Practice
S Peveri, S Pattini, M T Costantino, C Incorvaia, M Montagni, C Roncallo, D Villalta, E Savi
BACKGROUND: Component resolved diagnosis (CRD) allows to precisely identify the sensitization to specific molecules of a given allergenic source, resulting in an important improvement in clinical management, particularly of polysensitized subjects. This will end in the correct prescription of allergen immunotherapy (AIT) for respiratory allergy and in adequate avoidance diets or prescription of self-injectable adrenaline in food allergy. OBJECTIVE: The aim of this multicenter, real life study is to evaluate the percentage change of the diagnostic-therapeutic choice in polysensitized patients with respiratory allergy and in patients with food allergy, after using CRD compared to a first level diagnosis, along with an economic analysis of the patient's overall management according to the two different approaches...
September 20, 2018: Allergologia et Immunopathologia
P J Sarate, S Heinl, S Poiret, M Drinić, C Zwicker, I Schabussova, C Daniel, U Wiedermann
Allergic poly-sensitization affects a large number of allergic patients and poses a great challenge for their treatment. In this study we evaluated the effects of the probiotic Escherichia coli Nissle 1917 (EcN) expressing a birch and grass pollen allergen chimera 'Bet v 1, Phl p 1 and Phl p 5' (EcN-Chim) on allergy prevention after oral or intranasal application in poly-sensitized mice. In contrast to oral application, intranasal pretreatment with EcN-Chim prior to poly-sensitization led to a significant reduction of lung inflammation (eosinophils, IL-5, and IL-13 in bronchoalveolar lavage) along with suppressed levels of allergen-specific serum IgE...
September 21, 2018: Mucosal Immunology
Pinya Pulsawat, Tewarit Songrung, Pattraporn Satitsuksanoa, Maxime Le Mignon, Souad Khemili, Dimitri Gilis, Emmanuel Nony, Malcolm W Kennedy, Alain Jacquet
BACKGROUND: Protein crystallographic studies suggest that the house dust mite (HDM) allergen Der p 5 potentially interacts with hydrophobic ligands. Der p 5, in association with its ligand(s), might therefore trigger innate immune signaling pathways in the airway epithelium and influence the initiation of the HDM-allergic response. OBJECTIVE: We investigated the lipid-binding propensities of recombinant (r)Der p 5 and characterized the signaling pathways triggered by the allergen in airway epithelial cells...
September 19, 2018: Clinical and Experimental Allergy: Journal of the British Society for Allergy and Clinical Immunology
Yanhui Wen, Lifeng Zhou, Yuqiu Li, Zhengmin Li, Weijun Deng, Tao Zhang
OBJECTIVE: Increasing evidence suggests that leptin is upregulated during allergic reactions in the airway and related to the severity of disease in allergic rhinitis (AR). In this study, we aimed to investigate the expression of leptin during sublingual immunotherapy (SLIT) in AR patients. METHODS: Forty AR patients without obesity were recruited in this study. Twenty patients received house dust mite (HDM) allergen extract for SLIT and twenty patients received placebo randomly...
September 14, 2018: European Archives of Oto-rhino-laryngology
Danilo Villalta, Enrico Scala, Gianni Mistrello, Stefano Amato, Riccardo Asero
Seed storage proteins are extremely stable allergens in nuts, seeds, and legumes and are responsible for the most severe allergic reactions to these foods. The cross-reactivity between seed storage proteins from different sources has not been studied at a molecular level so far. This study aimed to ascertain the cross-reactivity between walnut and hazelnut seed storage proteins using recombinant allergens. Sera from 13 consecutive patients with severe primary walnut and/or hazelnut allergy and hypersensitive to both nuts were studied...
September 13, 2018: International Archives of Allergy and Immunology
Hela Safi, Andrea Wangorsch, Jonas Lidholm, Faiçal Brini, Jelena Spiric, Hans-Peter Rihs, Stefan Vieths, Alicia Armentia, Laura Farioli, Araceli Diaz-Perales, Elide Anna Pastorello, Stephan Scheurer
BACKGROUND: Common wheat (Triticum aestivum) and durum wheat (Triticum turgidum) are both involved in baker's asthma (BA) and food allergy (FA) including WDEIA. However, allergens in durum wheat have not been described, and the over-expression of T. turgidum nsLTPs is considered to increase resistance to phytopathogens. OBJECTIVE: To identify and assess the allergenicity of nsLTP from T. turgidum. METHODS: Recombinant T. turgidum nsLTP Tri tu 14 was generated and tested for structural integrity (CD-spectroscopy) and purity (SDS-PAGE)...
September 10, 2018: Clinical and Experimental Allergy: Journal of the British Society for Allergy and Clinical Immunology
Sejal Saglani, Lisa G Gregory, Avneet K Manghera, William J Branchett, Faith Uwadiae, Lewis J Entwistle, R A Oliver, Jessica E Vasiliou, Rebekah Sherburn, Stephen Lui, F Puttur, David Vöhringer, Simone A Walker, James Buckley, Ruth Grychtol, Valentina Fainardi, Laura Denney, Adam Byrne, Erika von Mutius, Andrew Bush, Clare M Lloyd
Airway hyperresponsiveness (AHR) is a critical feature of wheezing and asthma in children, but the initiating immune mechanisms remain unconfirmed. We demonstrate that both recombinant interleukin-33 (rIL-33) and allergen [house dust mite (HDM) or Alternaria alternata ] exposure from day 3 of life resulted in significantly increased pulmonary IL-13+ CD4+ T cells, which were indispensable for the development of AHR. In contrast, adult mice had a predominance of pulmonary Linneg CD45+ CD90+ IL-13+ type 2 innate lymphoid cells (ILC2s) after administration of rIL-33...
September 7, 2018: Science Immunology
Hathairat Srisong, Sophida Sukprasert, Sompong Klaynongsruang, Jureerut Daduang, Sakda Daduang
Background: Fire ant venom is a complex mixture consisting of basic piperidine alkaloids, various biologically active peptides and protein components, including a variety of major allergenic proteins. Tropical fire ant Solenopsis geminata is an important stinging ant species that causes anaphylaxis and serious medical problems. Although the biological activities of allergenic venom proteins that are unique to ant venom, particularly Solenopsis 2 and 4, are still unknown, these proteins are believed to play important roles in mediating the effects of the piperidine derivatives in the venom...
2018: Journal of Venomous Animals and Toxins Including Tropical Diseases
Lorenz G Stock, Sabrina Wildner, Christof Regl, Gabriele Gadermaier, Christian G Huber, Hanno Stutz
The response to thermal stress is an important parameter relevant for characterizing the biological activity and long-term stability of recombinant proteins, which may show irreversible, pH dependent structural changes under these conditions. We selected the recombinant pollen allergen of mugwort ( Artemisia vulgaris) rArt v 3.0201 as a relevant model to study structural changes due to thermal and pH stress by means of capillary zone electrophoresis (CZE)-UV and capillary zone electrophoresis (CZE)-electrospray ionization (ESI)-TOF-MS...
October 3, 2018: Analytical Chemistry
Carmen Oeo-Santos, Salvador Mas, Joaquín Quiralte, Carlos Colás, Miguel Blanca, Javier Fernández, Francisco Feo Brito, Mayte Villalba, Rodrigo Barderas
BACKGROUND: Cross-reactivity reactions between allergenic polygalacturonases (PGs) from different biological sources, especially foods and pollens from the Oleaceae family, have been described using Salsola kali PG (Sal k 6). No PG from olive pollen has been characterized to date, hampering further knowledge about cross-reactions through PGs. OBJECTIVES: The aim of this work was to determine the potential allergenicity of the PG from olive pollen and clarify its role in cross-reactivity...
August 31, 2018: International Archives of Allergy and Immunology
Sachi Minagawa, Yuzuru Nakaso, Masahiro Tomita, Takenori Igarashi, Yoshio Miura, Hideyo Yasuda, Satoshi Sekiguchi
BACKGROUND: The generation of recombinant proteins for commercialisation must be cost-effective. Despite the cost-effective production of recombinant feline interferon (rFeIFN) by a baculovirus expression system, this rFeIFN carries insect-type N-glycans, with core α 1,3 fucosyl residues that act as potential allergens. An alternative method of production may yield recombinant glycoproteins with reduced antigenicity. RESULTS: A cDNA clone encoding the fifteenth subtype of FeIFN-α (FeIFN-α15) was isolated from a Japanese domestic cat...
August 31, 2018: BMC Veterinary Research
Jill Glesner, Stephanie Filep, Lisa D Vailes, Sabina Wünschmann, Martin D Chapman, Giovanni Birrueta, April Frazier, Kyoung Yong Jeong, Coby Schal, Leonard Bacharier, Avraham Beigelman, Paula Busse, Véronique Schulten, Alex Sette, Anna Pomés
BACKGROUND: Cockroach allergens are an important cause of IgE-mediated sensitization in inner-city asthmatic patients. However, cockroach extracts used for diagnosis and immunotherapy are not standardized. OBJECTIVE: We sought to determine the allergen content of nonstandardized German cockroach extracts and the levels of sensitization to an expanded set of cockroach allergens as determinants of in vitro extract potency for IgE reactivity. METHODS: Twelve German cockroach extracts were compared for allergen content and potency of IgE reactivity...
August 28, 2018: Journal of Allergy and Clinical Immunology
Alessandro Mangogna, Beatrice Belmonte, Chiara Agostinis, Giuseppe Ricci, Alessandro Gulino, Ines Ferrara, Fabrizio Zanconati, Claudio Tripodo, Federico Romano, Uday Kishore, Roberta Bulla
Surfactant protein D (SP-D) is a pattern recognition molecule belonging to the Collectin (collagen-containing C-type lectin) family that has pulmonary as well as extra-pulmonary existence. In the lungs, it is a well-established opsonin that can agglutinate a range of microbes, and enhance their clearance via phagocytosis and super-oxidative burst. It can interfere with allergen-IgE interaction and suppress basophil and mast cell activation. However, it is now becoming evident that SP-D is likely to be an innate immune surveillance molecule against tumor development...
2018: Frontiers in Immunology
Tristan Hayes, Amanda Rumore, Brad Howard, Xin He, Mengyao Luo, Sabina Wuenschmann, Martin Chapman, Shiv Kale, Liwu Li, Hirohito Kita, Christopher B Lawrence
Allergens are molecules that elicit a hypersensitive inflammatory response in sensitized individuals and are derived from a variety of sources. Alt a 1 is the most clinically important secreted allergen of the ubiquitous fungus, Alternaria . It has been shown to be a major allergen causing IgE-mediated allergic response in the vast majority of Alternaria -sensitized individuals. However, no studies have been conducted in regards to the innate immune eliciting activities of this clinically relevant protein. In this study, recombinant Alt a 1 was produced, purified, labeled, and incubated with BEAS-2B, NHBE, and DHBE human lung epithelial cells...
2018: Frontiers in Immunology
Arthur H Totten, Li Xiao, Danlin Luo, David Briles, Joanetha Y Hale, Donna M Crabb, Trenton R Schoeb, Ammar Saadoon Alishlash, Ken B Waites, T Prescott Atkinson
BACKGROUND: Mycoplasma pneumoniae, an atypical human pathogen, has been associated with asthma initiation and exacerbation. Asthmatic patients have been reported to have higher carriage rates of M pneumoniae compared with nonasthmatic subjects and are at greater risk for invasive respiratory infections. OBJECTIVE: We sought to study whether prior allergen sensitization affects the host response to chronic bacterial infection. METHODS: BALB/cJ and IL-4 receptor α-/- mice were sensitized with ovalbumin (OVA) and then infected with M pneumoniae or Streptococcus pneumoniae...
August 6, 2018: Journal of Allergy and Clinical Immunology
Mey-Fann Lee, Chu-Hui Chiang, Ying-Lan Li, Nancy M Wang, Pei-Pong Song, Shyh-Jye Lin, Yi-Hsing Chen
BACKGROUND: American cockroaches (Periplaneta americana) are an important indoor allergen source and a major risk factor for exacerbations and poor control of asthma. We previously reported that allergen components from American cockroaches exhibit varying levels of pathogenicity. Sensitization to major American cockroach allergen, Per a 2, correlated with more severe clinical phenotypes among patients with allergic airway diseases. MATERIALS AND METHODS: In this study, we examined whether oral plant vaccine-encoding full-length Per a 2 clone-996 or its hypoallergenic clone-372 could exert a prophylactic role in Per a 2-sensitized mice...
2018: PloS One
Kashinath Bhattacharya, Gaurab Sircar, Angira Dasgupta, Swati Gupta Bhattacharya
The growing prevalence of allergy and asthma in India has become a major health concern with symptoms ranging from mild rhinitis to severe asthma and even life-threatening anaphylaxis. The "allergen repertoire" of this subcontinent is highly diverse due to the varied climate, flora, and food habits. The proper identification, purification, and molecular characterization of allergy-eliciting molecules are essential in order to facilitate an accurate diagnosis and to design immunotherapeutic vaccines...
July 27, 2018: International Archives of Allergy and Immunology
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