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https://www.readbyqxmd.com/read/28743125/surfactant-protein-b-suppresses-lung-cancer-progression-by-inhibiting-secretory-phospholipase-a2-activity-and-arachidonic-acid-production
#1
Sungmin Lee, Daehoon Kim, JiHoon Kang, EunGi Kim, Wanyeon Kim, HyeSook Youn, BuHyun Youn
BACKGROUND/AIMS: Radiotherapy is applied to patients with inoperable cancer types including advanced stage non-small cell lung cancer (NSCLC) and radioresistance functions as a critical obstacle in radiotherapy. This study was aimed to investigate the mechanism of radioresistance regulated by surfactant protein B (SP-B). METHODS: To investigate the role of SP-B in radioresistance, ΔSFTPB A549 cell line was established and SP-B expression was analyzed. In response to ionizing radiation (IR), the change of SP-B expression was analyzed in A549 and NCI-H441 cell lines...
July 25, 2017: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/28743101/in-silico-locating-the-immune-reactive-segments-of-lepidium-draba-peroxidase-and-designing-a-less-immune-reactive-enzyme-derivative
#2
Yaser Fattahian, Ali Riahi-Madvar, Reza Mirzaee, Gholamreza Asadikaram, Mohammad Reza Rahbar
Peroxidases have broad applications in industry, environmental as well as pharmaceutical and diagnosis. Recently applicability of peroxidases in cancer therapy was mentioned. In the present study, a horseradish peroxidase homologue from Lepidium draba was subjected to in silico analyzes aiming at identifying and locating immune-reactive regions. A derivative sequence with decreased immunogenicity and increased stability also suggested. The tertiary structure of the enzyme was predicted. The functional and structural importance of residues was annotated as well as the conservatory status of each residue...
July 17, 2017: Computational Biology and Chemistry
https://www.readbyqxmd.com/read/28743100/studies-on-secondary-metabolite-profiling-anti-inflammatory-potential-in-vitro-photoprotective-and-skin-aging-related-enzyme-inhibitory-activities-of-malaxis-acuminata-a-threatened-orchid-of-nutraceutical-importance
#3
Biswajit Bose, Hiranjit Choudhury, Pramod Tandon, Suman Kumaria
Malaxis acuminata D. Don., a small, terrestrial orchid, is endemic to tropical Himalayas at an altitude of 1200-2000m asl. The dried pseudobulbs are important ingredients of century old ayurvedic drug 'Ashtavarga' and a polyherbal immune-booster nutraceutical 'Chyavanprash', known to restore vigour, vitality and youthfulness. Considering tremendous medicinal importance of this threatened orchid species, a detailed study was undertaken for the first time to address its antioxidant potential, secondary metabolite contents and biological activities against skin-aging related enzymes (anti-collagenase, anti-elastase, anti-tyrosinase and xanthine oxidase) and anti-inflammatory activity (5-lipoxygenase and hyaluronidase) in different plant parts of wild and in vitro-derived plants of M...
July 12, 2017: Journal of Photochemistry and Photobiology. B, Biology
https://www.readbyqxmd.com/read/28743091/performance-comparison-of-two-herbal-and-industrial-medicines-using-nanoparticles-with-a-starch-cellulose-shell-and-alginate-core-for-drug-delivery-in-vitro-studies
#4
Akbar Esmaeili, Sahar Behzadi
In this study, the performance of two kinds of industrial and herbal drugs encapsulated in nanoparticles with a shell of starch and cellulose and an alginate core were examined as a new technique for nanoparticle drug delivery. The test method involved creating a suspension of starch and alginate, which was then dried, mixed with cellulose, and heated to form core-shell nanoparticles. The industrial drug calcitonin and an extract of the herb Amaranthus retroflexcus L. were added separately and in combination to the nanoparticles, and the performance of each configuration was evaluated...
July 18, 2017: Colloids and Surfaces. B, Biointerfaces
https://www.readbyqxmd.com/read/28743090/nir-and-uv-responsive-degradable-hyaluronic-acid-nanogels-for-cd44-targeted-and-remotely-triggered-intracellular-doxorubicin-delivery
#5
Chunfeng Hang, Yan Zou, Yinan Zhong, Zhiyuan Zhong, Fenghua Meng
Hyaluronic acid (HA) is an endogenous polysaccharide that shows intrinsic targetability to CD44+ cancer cells. Here, we developed NIR and UV-responsive degradable nanogels from hyaluronic acid-g-7-N,N-diethylamino-4-hydroxymethylcoumarin (HA-CM) for CD44 targeted and remotely controlled intracellular doxorubicin (DOX) delivery. Nanometer-sized HA-CM nanogels could readily load DOX, and both NIR and UV irradiation could significantly enhance DOX release from the nanogels, resulting from light-triggered cleavage of urethane bonds that connect CM to HA...
July 18, 2017: Colloids and Surfaces. B, Biointerfaces
https://www.readbyqxmd.com/read/28743081/mycobacterium-tuberculosis-ppe44-rv2770c-is-involved-in-response-to-multiple-stresses-and-promotes-the-macrophage-expression-of-il-12-p40-and-il-6-via-the-p38-erk-and-nf-%C3%AE%C2%BAb-signaling-axis
#6
Zhaoxiao Yu, Chenhui Zhang, Mingliang Zhou, Qiming Li, Hui Li, Wei Duan, Xue Li, Yonghong Feng, Jianping Xie
Tuberculosis (TB), caused by Mycobacterium tuberculosis, remains a formidable threat to global public health. The successful intracellular persistence of M. tuberculosis significantly contributes to the intractability of tuberculosis. Proline-glutamic acid (PE) and proline-proline-glutamic acid (PPE) are mycobacterial exclusive protein families that widely reported to be involved in the bacterial virulence, physiology and interaction with host. Rv2770c (PPE44), a predicted virulence factor, was up-regulated upon the infected guinea pig lungs...
July 22, 2017: International Immunopharmacology
https://www.readbyqxmd.com/read/28743079/impact-of-lyophilized-lactobacillus-salivarius-dspv-001p-administration-on-growth-performance-microbial-translocation-and-gastrointestinal-microbiota-of-broilers-reared-under-low-ambient-temperature
#7
J E Blajman, C A Olivero, M L Fusari, J A Zimmermann, E Rossler, A P Berisvil, A Romero Scharpen, D M Astesana, L P Soto, M L Signorini, M V Zbrun, L S Frizzo
This study was undertaken with the aim of investigating the effects of dietary supplementation of probiotic strain Lactobacillus salivarius DSPV 001P on growth performance, microbial translocation, and gastrointestinal microbiota of broilers reared under low ambient temperature. Two hundred and forty, one-day-old male Cobb broilers were randomly distributed into two treatment groups, a probiotic group and a control group, with four replicates per treatment and 30 broilers per replicate. The temperature of the broiler house was maintained at 18-22°C during the first three weeks, after which the temperature was at range of 8°C to 12°C...
July 19, 2017: Research in Veterinary Science
https://www.readbyqxmd.com/read/28743076/lc-ms-ms-esi-method-for-simultaneous-quantification-of-darolutamide-and-its-active-metabolite-orm-15341-in-mice-plasma-and-its-application-to-a-pharmacokinetic-study
#8
Sreekanth Dittakavi, Pavan Kumar V S P Nagasuri, Suresh P Sulochana, Syed Mohd Saim, Sadanand R Mallurwar, Mohd Zainuddin, Purushottam Dewang, Sriram Rajagopal, Ramesh Mullangi
A sensitive and rapid LC-MS/MS method was developed and validated for the simultaneous quantitation of darolutamide and its active metabolite i.e. ORM-15341 in 50μL mice plasma using bicalutamide as an internal standard (I.S.) as per regulatory guidelines. Sample processing was accomplished through liquid-liquid extraction. Chromatographic separation was achieved using an Atlantis C18 column with an isocratic mobile phase comprising 0.2% formic acid:acetonitrile (35:65, v/v) at a flow rate of 0.8mL/min within 2...
July 13, 2017: Journal of Pharmaceutical and Biomedical Analysis
https://www.readbyqxmd.com/read/28743075/preference-of-arabidopsis-thaliana-gh3-5-acyl-amido-synthetase-for-growth-versus-defense-hormone-acyl-substrates-is-dictated-by-concentration-of-amino-acid-substrate-aspartate
#9
Rebecca Mackelprang, Rachel A Okrent, Mary C Wildermuth
The GH3 family of adenylating enzymes conjugate acyl substrates such as the growth hormone indole-3-acetic acid (IAA) to amino acids via a two-step reaction of acyl substrate adenylation followed by amino acid conjugation. Arabidopsis thaliana GH3.5 was previously shown to be unusual in that it could adenylate both IAA and the defense hormone salicylic acid (SA, 2-hydroxybenzoate). Our detailed studies of the kinetics of GH3.5 on a variety of auxin and benzoate substrates provides insight into the acyl preference and reaction mechanism of GH3...
July 22, 2017: Phytochemistry
https://www.readbyqxmd.com/read/28743072/molecular-modeling-and-molecular-dynamics-simulation-study-of-archaeal-leucyl-trna-synthetase-in-complex-with-different-mischarged-trna-in-editing-conformation
#10
A V Rayevsky, M Sharifi, M A Tukalo
Aminoacyl-tRNA synthetases (aaRSs) play important roles in maintaining the accuracy of protein synthesis. Some aaRSs accomplish this via editing mechanisms, among which leucyl-tRNA synthetase (LeuRS) edits non-cognate amino acid norvaline mainly by post-transfer editing. However, the molecular basis for this pathway for eukaryotic and archaeal LeuRS remain unclear. In this study, a complex of archaeal P. horikoshii LeuRS (PhLeuRS) with misacylated tRNA(Leu) was modeled wherever tRNA's acceptor stem was oriented directly into the editing site...
July 18, 2017: Journal of Molecular Graphics & Modelling
https://www.readbyqxmd.com/read/28743071/prediction-of-protein-structural-class-for-low-similarity-sequences-using-chou-s-pseudo-amino-acid-composition-and-wavelet-denoising
#11
Bin Yu, Lifeng Lou, Shan Li, Yusen Zhang, Wenying Qiu, Xue Wu, Minghui Wang, Baoguang Tian
Prediction of protein structural class plays an important role in protein structure and function analysis, drug design and many other biological applications. Prediction of protein structural class for low-similarity sequences is still a challenging task. Based on the theory of wavelet denoising, this paper presents a novel method of prediction of protein structural class for the first time. Firstly, the features of the protein sequence are extracted by using Chou's pseudo amino acid composition (PseAAC). Then the extracted feature information is denoised by two-dimensional (2D) wavelet...
July 14, 2017: Journal of Molecular Graphics & Modelling
https://www.readbyqxmd.com/read/28743050/pml-rar%C3%AE-stabilized-by-zinc-in-human-acute-promyelocytic-leukemia-nb4-cells
#12
Bo Zhu, Jia-Yu Wang, Jun-Jie Zhou, Feng Zhou, Wei Cheng, Ying-Ting Liu, Jie Wang, Xiao Chen, Dian-Hua Chen, Lan Luo, Zi-Chun Hua
Acute promyelocytic leukemia (APL) is characterized and driven by the promyelocytic leukemia protein-retinoic acid receptor alpha (PML-RARα) fusion gene. Previous studies have highlighted the importance of PML-RARα degradation in the treatment against APL. Considering the presence of two zinc fingers in the PML-RARα fusion protein, we explored the function of zinc homeostasis in maintaining PML-RARα stability. We demonstrated for the first time that zinc depletion by its chelator N,N,N',N'-tetrakis(2-pyridylmethyl)ethylenediamine (TPEN) triggered PML-RARα degradation in NB4 APL cells via the proteasome pathway rather than the autophagy-lysosomal pathway...
July 19, 2017: Journal of Inorganic Biochemistry
https://www.readbyqxmd.com/read/28743039/exogenous-application-of-poly-%C3%AE-glutamic-acid-enhances-stress-defense-in-brassica-napus-l-seedlings-by-inducing-cross-talks-between-ca-2-h2o2-brassinolide-and-jasmonic-acid-in-leaves
#13
Zongqi Xu, Peng Lei, Xiao Pang, Huashan Li, Xiaohai Feng, Hong Xu
Poly-γ-glutamic acid (γ-PGA) is a microbe-secreted isopeptide shown to promote growth and enhance crop stress tolerance. However, its downstream signaling pathways are unknown. Here, we studied γ-PGA-induced tolerance to salt and cold stresses. Pretreatment with γ-PGA contributed to enhance stress tolerance of canola seedlings by promoting proline accumulation and total antioxidant capacity (T-AOC) improvement. Further, Ca(2+), H2O2, brassinolide, and jasmonic acid were found to be involved in the γ-PGA-induced process...
July 17, 2017: Plant Physiology and Biochemistry: PPB
https://www.readbyqxmd.com/read/28742971/evidence-for-quinone-redox-chemistry-mediating-daytime-and-nighttime-no2-to-hono-conversion-on-soil-surfaces
#14
Nicole K Scharko, Erin T Martin, Yaroslav B Losovyj, Dennis G Peters, Jonathan D Raff
Humic acid (HA) is thought to promote NO2 conversion to nitrous acid (HONO) on soil surfaces during the day. However, it has proven difficult to identify the reactive sites in natural HA substrates. The mechanism of NO2 reduction on soil surrogates comprised of HA and clay minerals was studied by use of a coated-wall flow reactor coupled to cavity-enhanced spectroscopy. Conversion of NO2 to HONO in the dark was found to be significant and correlated to the abundance of C‒O moieties in HA determined from X-ray photoelectron spectra of the C1s region...
July 25, 2017: Environmental Science & Technology
https://www.readbyqxmd.com/read/28742969/fabrication-of-a-new-lineage-of-artificial-luciferases-from-natural-luciferase-pools
#15
Sung Bae Kim, Ryo Nishihara, Daniel Citterio, Koji Suzuki
The fabrication of artificial luciferases (ALucs) with unique optical properties can have a fundamental impact on bioassays and molecular imaging. In this study, we developed a new lineage of ALucs with unique substrate preferences by extracting consensus amino acids from the alignment of 25 copepod luciferase sequences available in published databases. The primary sequence was first created with a sequence logo generator resulting in a total of 11 sibling sequences. Phylogenetic analysis shows that the newly fabricated ALucs form an independent branch, genetically isolated from the natural luciferases and from a prior series of ALucs produced by our laboratory using a smaller basis set...
July 25, 2017: ACS Combinatorial Science
https://www.readbyqxmd.com/read/28742967/molybdenum-0-dihapto-coordination-of-benzene-and-trifluorotoluene-the-stabilizing-and-chemo-directing-influence-of-a-cf3-group
#16
Jeffery T Myers, Jacob A Smith, Steven J Dakermanji, Justin H Wilde, Katy B Wilson, Philip J Shivokevich, W Dean Harman
The preparation of the complex TpMo(NO)(DMAP)(η2-PhCF3) (5) and TpMo(NO)(DMAP)(η2-benzene) (3) are described. The CF3 group is found to stabilize the metal-arene bond strength in 5 by roughly 3 kcal/mol, compared to 3, allowing the large-scale synthesis and isolation of the trifluorotoluene analog (5, 37 g, 70%). When a benzene solution of 5 is allowed to stand, clean conversion to the benzene analog 3 occurs, and this complex may be precipitated from solution upon the addition of pentane, and isolated...
July 25, 2017: Journal of the American Chemical Society
https://www.readbyqxmd.com/read/28742940/functional-analysis-of-the-pepper-protein-phosphatase-caaipp1-and-its-interacting-partner-caairf1-modulation-of-aba-signaling-and-the-drought-stress-response
#17
Woonhee Baek, Chae Woo Lim, Sung Chul Lee
Plant adaptive responses to abiotic stress are coordinated by restriction of plant growth and development. The plant hormone abscisic acid (ABA) is the key regulator of the response to abiotic stress, and its sensitivity determines abiotic stress tolerance levels. We previously showed that the E3 ubiquitin ligase CaAIRF1 functions as a positive regulator of ABA and drought stress via modulation of transcription and stability of the type 2C protein phosphatase CaADIP1. Here, we report the identification and functional analysis of a novel type 2C phosphatase, CaAIPP1 (Capsicum annuum CaAIRF1 Interacting Protein Phosphatase 1)...
July 25, 2017: Plant, Cell & Environment
https://www.readbyqxmd.com/read/28742939/disparate-peroxisome-related-defects-in-arabidopsis-pex6-and-pex26-mutants-link-peroxisomal-retrotranslocation-and-oil-body-utilization
#18
Kim L Gonzalez, Wendell A Fleming, Yun-Ting Kao, Zachary J Wright, Savina V Venkova, Meredith J Ventura, Bonnie Bartel
Catabolism of fatty acids stored in oil bodies is essential for Arabidopsis seed germination and seedling development. This fatty acid breakdown occurs in peroxisomes, organelles that sequester oxidative reactions. Import of peroxisomal enzymes is facilitated by peroxins including PEX5, a receptor that delivers cargo proteins from the cytosol to the peroxisomal matrix. After cargo delivery, a complex of the PEX1 and PEX6 ATPases and the PEX26 tail-anchored membrane protein removes ubiquitinated PEX5 from the peroxisomal membrane...
July 25, 2017: Plant Journal: for Cell and Molecular Biology
https://www.readbyqxmd.com/read/28742934/efficient-photoelectrochemical-reduction-of-co2-to-formic-acid-with-functionalized-ionic-liquid-as-absorbent-and-electrolyte
#19
Weiwei Lu, Bo Jia, Beilei Cui, Yuan Zhang, Kaisheng Yao, Yuling Zhao, Jian Ji Wang
Photoelectrochemical (PEC) reduction of CO2 is a potential way to produce fuels and chemicals by using C1-feedstock accumulated in the atmosphere. However, low solubility of CO2 in water and complicated processes of capture and conversion make the conversion efficiency quite low. In this study, a new conception of "ionic liquid assisted capture and conversion of CO2 in PEC system" is proposed and demonstrated by using 1-aminopropyl-3-methylimidazolium bromide aqueous solution as both the absorbent and electrolyte at ambient temperature and pressure...
July 25, 2017: Angewandte Chemie
https://www.readbyqxmd.com/read/28742883/effects-of-oral-florfenicol-and-azithromycin-on-gut-microbiota-and-adipogenesis-in-mice
#20
Rui Li, Hexing Wang, Qingfeng Shi, Na Wang, Zhijie Zhang, Chenglong Xiong, Jianxiang Liu, Yue Chen, Lufang Jiang, Qingwu Jiang
Certain antibiotics detected in urine are associated with childhood obesity. In the current experimental study, we investigated two representative antibiotics detected in urine, florfenicol and azithromycin, for their early effects on adipogenesis, gut microbiota, short-chain fatty acids (SCFAs), and bile acids in mice. Thirty C57BL/6 mice aged four weeks were randomly divided into three groups (florfenicol, azithromycin and control). The two experimental groups were administered florfenicol or azithromycin at 5 mg/kg/day for four weeks...
2017: PloS One
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