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Masaki Naganuma, Yuichiro Inatomi, Makoto Nakajima, Toshiro Yonehara, Yukio Ando
BACKGROUND: Uric acid (UA), an antioxidant with neuroprotective effects, favorably affects stroke outcome. However, the effect has not been examined in patients treated with edaravone, a frequently used free radical scavenger. We investigated whether the use of edaravone affected the relationship between UA levels and outcome in acute ischemic stroke. METHODS: We retrospectively evaluated 1,114 consecutive ischemic stroke patients with premorbid modified Rankin Scale (mRS) scores <2 admitted within 24 h of onset (mean, 74 years; median UA levels, 333 μmol/L)...
March 20, 2018: Cerebrovascular Diseases
Yilin Sun, Le Zhen, Ying Peng, Jiankun Wang, Fei Fei, Lixiang Aa, Wenjiao Jiang, Xue Pei, Li Lu, Jie Liu, Guangji Wang, Kun Hao
To improve bioavailability and provide resistance to deamination, an array of gemcitabine (dFdC) prodrugs carrying the acyl modifications has been successful in the optimization of pharmacokinetic properties of dFdC, but the reports about 4-N-carbobenzoxy-dFdC (Cbz-dFdC), a dFdC prodrug bearing alkyloxycarbonyl modification, are relatively rare. Notably, in vivo enzymatic hydrolysis was an absolutely essential factor for the activation of these prodrugs, which is correlated with the anti-tumor activity. Therefore, detailed metabolism studies of Cbz-dFdC should be carried out for a more authentic pharmacodynamic evaluation...
March 14, 2018: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
Dai Long Vu, Karolína Ranglová, Jan Hájek, Pavel Hrouzek
Quantification of selenated amino-acids currently relies on methods employing inductively coupled plasma mass spectrometry (ICP-MS). Although very accurate, these methods do not allow the simultaneous determination of standard amino-acids, hampering the comparison of the content of selenated versus non-selenated species such as methionine (Met) and selenomethionine (SeMet). This paper reports two approaches for the simultaneous quantification of Met and SeMet. In the first approach, standard enzymatic hydrolysis employing Protease XIV was applied for the preparation of samples...
March 9, 2018: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
Xiao-Mei Shi, Gao-Chao Fan, Xueying Tang, Qingming Shen, Jun-Jie Zhu
Sensitive and specific detection of DNA is of great significance for clinical diagnosis. In this paper, an effective cascade signal amplification strategy was introduced into photoelectrochemical (PEC) biosensor for ultrasensitive detection of human T-cell lymphotropic virus type I (HTLV-I) DNA. This proposed signal amplification strategy integrates λ-exonuclease (λ-Exo) aided target recycling with hybridization chain reaction (HCR) and enzyme catalysis. In the presence of target DNA (tDNA) of HTLV-I, the designed hairpin DNA (h1 DNA) hybridized with tDNA, subsequently recognized and cleaved by λ-Exo to set free tDNA...
March 13, 2018: Biosensors & Bioelectronics
Wen-Ping Huang, Ting Tan, Zhi-Feng Li, Hui OuYang, Xu Xu, Bin Zhou, Yu-Lin Feng
The Chimonanthus nitens Oliv. leaf (CNOL). is a widely used traditional Chinese medicine (TCM) used for treating colds and influenza. In the present study, a comprehensive strategy integrating multiple chromatographic analysis and chemometric methods was firstly proposed for structural characterization and discrimination of CNOL from different geographical origins. It consists of three steps: Firstly, the ultra-high-performance liquid chromatography quadrupole time-of-flight tandem mass spectrometry (UHPLC-QTOF-MS/MS) method was applied for comprehensive profiling of characterization constituents in CNOL by high-resolution diagnostic product ions/neutral loss filtering, and a total of 40 constituents were identified...
February 10, 2018: Journal of Pharmaceutical and Biomedical Analysis
Mahsa Mazloomi-Rezvani, Mehdi Salami-Kalajahi, Hossein Roghani-Mamaqani
Different core-shell nanoparticles with Au as core and stimuli-responsive polymers such as poly(acrylic acid) (PAA), poly(methacrylic acid) (PMAA), poly(N-isopropylacrylamide) (PNIPAAm), poly(N,N'-methylenebis(acrylamide)) (PMBA), poly(2-hydroxyethyl methacrylate) (PHEMA) and poly((2-dimethylamino)ethyl methacrylate) (PDMAEMA) as shells were fabricated via inverse emulsion polymerization. Dynamic light scattering (DLS) was used to investigate particles sizes and particle size distributions and transmission electron microscopy (TEM) was applied to observe the core-shell structure of Au-polymer nanoparticles...
March 13, 2018: Colloids and Surfaces. B, Biointerfaces
Morteza Karimzadeh, Neda Manouchehri, Dariush Saberi, Khodabakhsh Niknam
All 66 conformers of guanylurea were optimized and frequency calculations were performed at M06-2X/6-311++G(d,p) level of theory. Theses conformers were categorized into five tautomers, and the most stable conformer of each tautomer were found. Geometrical parameters indicated that these tautomers have almost planar structure. Complete stepwise tautomerism were studied through both intramolecular proton transfer routs and internal rotations. Results indicated that the proton transfer routs involving four-membered heterocyclic structures were rate-determining steps...
March 6, 2018: Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy
Min Cheng, Guangming Zeng, Danlian Huang, Cui Lai, Yang Liu, Chen Zhang, Jia Wan, Liang Hu, Chengyun Zhou, Weiping Xiong
The presence of antibiotics in aquatic environments has attracted global concern. Fenton process is an attractive yet challenging method for antibiotics degradation, especially when such a reaction can be conducted at neutral pH values. In this study, a novel composite Fe/Co catalyst was synthesized via the modification of steel converter slag (SCS) by salicylic acid-methanol (SAM) and cobalt nitrate (Co(NO3 )2 ). The catalysts were characterized by N2 -Brunauer-Emmett-Teller (BET), X-ray diffraction (XRD), Fourier transform infrared (FT-IR), X-ray photoelectron spectroscopy (XPS), scanning electron microscopy (SEM) and energy dispersive X-ray spectroscopy (EDS)...
March 10, 2018: Water Research
Lindsay Glicksberg, Sumandeep Rana, Sarah Kerrigan
PURPOSE: Synthetic cathinones are encountered in a variety of antemortem and postmortem forensic toxicology investigations. Earlier experimental studies using fortified urine have evaluated analyte, temperature and pH-dependent variables associated with their stability. The purpose of this study was to compare experimental findings with those obtained using authentic urine from cathinone users. METHODS: In this report we compare cathinone concentrations in 180 authentic unpreserved urine specimens, following known periods of refrigerated storage...
March 8, 2018: Forensic Science International
Ying Huang, Fang Liu, Fang Zhang, Pingsheng Liu, Tao Xu, Wenjun Ding
Numerous studies have demonstrated that vanadium compounds are able to improve lipemia and triglyceridemia in both humans and animals. However, the molecular mechanism remains elusive. The present study was conducted to investigate the anti-hyperlipidemic effect of vanadium(IV) complex with 4-chlorodipicolinic acid (VOdipic-Cl)-induced autophagy on hepatic lipid accumulation. To explore the possible underlying mechanisms, primary rat hepatocytes, human hepatoma cell line HepG2, and liver tissue from C57BL/6 mice fed a high-fat diet (HFD) were used...
March 14, 2018: Journal of Inorganic Biochemistry
Aziza Hadj Ltaïef, Simona Sabatino, Federica Proietto, Salah Ammar, Abdellatif Gadri, Alessandro Galia, Onofrio Scialdone
The treatment of toxic organic pollutants by electro-Fenton (EF) presents some drawbacks such as the necessity to work at low pH and the low solubility of oxygen in water contacted with air or oxygen at room pressure that results often in slow and relatively low abatements. Here, the coupled adoption of natural heterogeneous catalysts and of relatively high pressure was proposed in order to improve the performances of EF for the treatment of organic pollutants. Caffeic acid (CA) and 3-chlorophenol were used as model resistant organic pollutants...
March 12, 2018: Chemosphere
Burak DiK, Emre Bahcivan, Hatice Eser Faki, Kamil Uney
In the present study, combined treatment with etanercept and anakinra were tested in the streptozotocin-induced diabetic rats. Forty male Wistar albino rats were divided into 5 groups; healthy control (HC), diabetic control (DC), diabetic+anakinra (DAT), diabetic+etanercept (DET), and diabetic+etanercept+anakinra (DEAT). HC and DC groups received subcutaneous (sc.) injection with a saline solution, while DAT and DET groups received anakinra (10 mg/kg/day, sc.) or etanercept (10 mg/kg, twice a week, sc.), and DEAT rats received both anakinra and etanercept treatments for 21 days after diabetes has developed...
March 20, 2018: Canadian Journal of Physiology and Pharmacology
Geoff A Christensen, JiWon Moon, Allison M Veach, Jennifer J Mosher, Ann M Wymore, Joy D van Nostrand, Jizhong Zhou, Terry C Hazen, Adam P Arkin, Dwayne A Elias
Using in-field bioreactors, we investigated the influence of exogenous microorganisms in groundwater planktonic and biofilm microbial communities as part of the Integrated Field Research Challenge (IFRC). After an acclimation period with source groundwater, bioreactors received either filtered (0.22 μM filter) or unfiltered well groundwater in triplicate and communities were tracked routinely for 23 days after filtration was initiated. To address geochemical influences, the planktonic phase was assayed periodically for protein, organic acids, physico-/geochemical measurements and bacterial community (via 16S rRNA gene sequencing), while biofilms (i...
2018: PloS One
Vanesa Bellou, Lazaros Belbasis, Ioanna Tzoulaki, Evangelos Evangelou
BACKGROUND: Type 2 diabetes mellitus (T2DM) is a global epidemic associated with increased health expenditure, and low quality of life. Many non-genetic risk factors have been suggested, but their overall epidemiological credibility has not been assessed. METHODS: We searched PubMed to capture all meta-analyses and Mendelian randomization studies for risk factors of T2DM. For each association, we estimated the summary effect size, its 95% confidence and prediction interval, and the I2 metric...
2018: PloS One
Jeonghwan Lee, Young Lee, Boram Park, Sungho Won, Jin Suk Han, Nam Ju Heo
Chronic kidney disease (CKD) is an important social health problem characterized by a decrease in the kidney glomerular filtration rate (GFR). In this study, we analyzed genome-wide association studies for kidney disease-related traits using data from a Korean adult health screening cohort comprising 7,064 participants. Kidney disease-related traits analyzed include blood urea nitrogen (BUN), serum creatinine, estimated GFR, and uric acid levels. We detected two genetic loci (SLC14A2 and an intergenic region) and 8 single nucleotide polymorphisms (SNPs) associated with BUN, 3 genetic loci (BCAS3, C17orf82, ALDH2) and 6 SNPs associated with serum creatinine, 3 genetic loci (BCAS3, C17orf82/TBX2, LRP2) and 7 SNPs associated with GFR, and 14 genetic loci (3 in ABCG2/PKD2, 2 in SLC2A9, 3 in intergenic regions on chromosome 4; OTUB1, NRXN2/SLC22A12, CDC42BPG, RPS6KA4, SLC22A9, and MAP4K2 on chromosome 11) and 84 SNPs associated with uric acid levels...
2018: PloS One
Aftab Ahmad, Mohammed F Abuzinadah, Huda M Alkreathy, Babajan Banaganapalli, Mohd Mujeeb
BACKGROUND: Ursolic acid (UA) is a promising molecule with anti-inflammatory, analgesic and potential anti-arthritic activity. METHODS: This study was undertaken to make formulation and evaluation of Ocimum sanctum L. leaf extract (OLE) loaded nano-structured lipid carriers (OLE-NLCs) for improved transdermal delivery of UA. Different surfactants, solid lipids and liquid lipids were used for the preparation of NLCs. The NLCs were developed using emulsion solvent diffusion and evaporation method...
2018: PloS One
Jieun Yoon, Akihiro Kanamori, Keisuke Fujii, Hiroko Isoda, Tomohiro Okura
PURPOSE: Maslinic acid (MA) is a component derived from a natural olive-based extract known to have pharmacological functions that include suppressing inflammation. This study examined how MA, in conjunction with whole-body vibration training (WBVT), can improve knee and muscle function in elderly women with knee osteoarthritis (OA). METHODS: The study was a double-blinded, placebo-controlled, randomized intervention study that enrolled individuals with knee pain...
2018: PloS One
Chang Liu, Xueqin Ran, Jiafu Wang, Sheng Li, Jianfeng Liu
Genomic structural variation (SV) is noticed for the contribution to genetic diversity and phenotypic changes. Guizhou indigenous pig (GZP) has been raised for hundreds of years with many special characteristics. The present paper aimed to uncover the influence of SV on gene polymorphism and the genetic mechanisms of phenotypic traits for GZP. Eighteen GZPs were chosen for resequencing by Illumina sequencing platform. The confident SVs of GZP were called out by both programs of pindel and softSV simultaneously and compared with the SVs deduced from the genomic data of European pig (EUP) and the native pig outside of Guizhou, China (NPOG)...
2018: PloS One
Yoonha Kim, Bong-Gyu Mun, Abdul Latif Khan, Muhammad Waqas, Hyun-Ho Kim, Raheem Shahzad, Muhammad Imran, Byung-Wook Yun, In-Jung Lee
This study investigated the regulatory role of exogenous salicylic acid (SA) in rice and its effects on toxic reactive oxygen and nitrogen species during short-term salinity stress. SA application (0.5 and 1.0 mM) during salinity-induced stress (100 mM NaCl) resulted in significantly longer shoot length and higher chlorophyll and biomass accumulation than with salinity stress alone. NaCl-induced reactive oxygen species production led to increased levels of lipid peroxidation in rice plants, which were significantly reduced following SA application...
2018: PloS One
Balaram Mohapatra, Pinaki Sar, Sufia Khannam Kazy, Mrinal Kumar Maiti, Tulasi Satyanarayana
Reductive transformation of toxic arsenic (As) species by As reducing bacteria (AsRB) is a key process in As-biogeochemical-cycling within the subsurface aquifer environment. In this study, we have characterized a Gram-stain-negative, non-spore-forming, rod-shaped As reducing bacterium designated KAs 5-3T, isolated from highly As-contaminated groundwater of India. Strain KAs 5-3T displayed high 16S rRNA gene sequence similarity to the members of the genus Pseudoxanthomonas, with P. mexicana AMX 26BT (99.25% similarity), P...
2018: PloS One
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