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Long-Feng Lu, Shun Li, Zhao-Xi Wang, Shu-Bo Liu, Dan-Dan Chen, Yong-An Zhang
Viral infection activates the transcription factor IFN regulatory factor 7 (IRF7), which plays a critical role in the induction of IFNs and innate antiviral immune response. How virus-induced IFN signaling is controlled in fish is not fully understood. In this study, we demonstrate that N-myc downstream-regulated gene 1a (NDRG1a) in zebrafish plays a role as a negative regulator for virus-triggered IFN induction. First, the activation of the IFN promoter stimulated by the polyinosinic-polycytidylic acid or spring viremia of carp virus was decreased by the overexpression of NDRG1a...
November 30, 2018: Journal of Immunology: Official Journal of the American Association of Immunologists
George Daniel Grass, Matthew N Mills, Kamran A Ahmed, Casey L Liveringhouse, Michael J Montejo, Timothy J Robinson, Julio C Chavez, Louis B Harrison, Sungjune Kim
We investigated whether adding radiation (RT) to systemic therapy improved outcomes in early stage diffuse large B-cell lymphoma (DLBCL) patients with or without double- or triple-hit lymphoma (DHL/THL) biology. This analysis included 183 patients profiled with fluorescent in situ hybridization (FISH) for alterations in MYC, BLC2, and/or BCL6. A total of 146 (80%) were non-DHL/THL, 27 (15%) were DHL, and 10 (6%) were THL. Systemic therapy without RT resulted in inferior freedom from relapse (FFR) (HR: 2.28; 95% CI, 1...
November 20, 2018: Leukemia & Lymphoma
Fumiyoshi Fujishima, Hiroki Katsushima, Noriko Fukuhara, Sachiko Konosu-Fukaya, Yasuhiro Nakamura, Hajime Usubuchi, Satoko Sato, Yasunori Ota, Akiko Yashima-Abo, Tomohiro Nakamura, Naoki Nakaya, Hideo Harigae, Hironobu Sasano, Ryo Ichinohasama
Immunohistochemistry is not only the most important tool for pathologists to establish a final diagnosis but it can also inform decisions regarding optimal treatment methods. However, there is no universal standard notation for expressing immunohistochemical findings. For a diagnosis of malignant lymphoma, it is important to confirm the presence or absence of MYC translocation and communicate these results to a clinical audience. However, the criteria for selecting cases for fluorescence in situ hybridization (FISH) analysis to confirm MYC translocation are ill-defined...
November 15, 2018: Human Pathology
Akishi Ooi, Masafumi Inokuchi, Shin-Ichi Horike, Hiroko Kawashima, Satoko Ishikawa, Hiroko Ikeda, Ritsuko Nakamura, Takeru Oyama, Yoh Dobash
Gene amplification is a common event in breast cancer, and identifies actual and potential targets of molecular therapy. The aim of the present study was to determine the amplification status of 22 genes that are reportedly frequently amplified in breast cancers. An archive of 322 formalin-fixed and paraffin-embedded invasive breast cancer tissues were screened by multiple ligation-dependent probe amplification (MLPA) and a total of 906 gene loci judged as 'gain' or 'amplified' was further confirmed to have been amplified based on fluorescence in situ hybridization (FISH)...
October 29, 2018: Human Pathology
Ling Yuan, Michael C Kreissl, Liping Su, Zhifang Wu, Marcus Hacker, Jianzhong Liu, Xi Zhang, Yunfeng Bo, Hongyu Zhang, Xiang Li, Sijin Li
OBJECTIVES: 18 F-fluorodeoxyglucose (FDG) positron emission tomography (PET) is routinely used in diffuse large B cell lymphoma (DLBCL) for staging, assessment of remission and recurrence, and estimation of therapeutic efficacy. In this study, we aimed to assess the role of an early interim PET/computed tomography (CT) in the evaluation of response in DLBCL. METHODS: Sixty primary DLBCL patients (31 females) were analyzed. Baseline and follow-up 18 F-FDG PET/CT was performed in patients after one cycle (n = 30) and two cycles (n = 30) of chemotherapy...
October 31, 2018: European Journal of Nuclear Medicine and Molecular Imaging
Maja Perunicic Jovanovic, Biljana Mihaljevic, Petar Jovanovic, Jelena Jelicic, Vesna Cemerikic Martinovic, Jelica Jovanović, Marija Dencic Fekete, Milica Čekerevac, Nebojša Bojanić
Primary testicular diffuse large B-cell lymphoma (PT-DLBCL) represents a rare and aggressive extranodal non-Hodgkin's lymphoma (NHL) with some specific features that differ from other NHLs. Formalin fixed, paraffin wax embedded (FFPE) samples of 21 PT-DLBCLs and 30 comparative patients with DLBCL were analysed. All PT-DLBCL patients were treated with rituximab-containing regimens, intrathecal prophylaxis (10 patients), and irradiation of the contralateral testis (9 patients). FFPE samples were additionally analysed by immunohistochemistry (Bcl-2, c-Myc protein expression) and fluorescence in situ hybridisation (FISH) (BCL2 and MYC)...
2018: Polish Journal of Pathology: Official Journal of the Polish Society of Pathologists
Lauren C Chong, Susana Ben-Neriah, Graham W Slack, Ciara Freeman, Daisuke Ennishi, Anja Mottok, Brett Collinge, Pau Abrisqueta, Pedro Farinha, Merrill Boyle, Barbara Meissner, Robert Kridel, Alina S Gerrie, Diego Villa, Kerry J Savage, Laurie H Sehn, Reiner Siebert, Ryan D Morin, Randy D Gascoyne, Marco A Marra, Joseph M Connors, Andrew J Mungall, Christian Steidl, David W Scott
Genomic rearrangements in the MYC locus occur in ∼12% of lymphomas with diffuse large B-cell lymphoma (DLBCL) morphology and are associated with inferior outcome. Previous studies exploring MYC rearrangements have primarily used fluorescence in situ hybridization (FISH) assays to characterize break-apart status but have rarely examined breakpoint location, and in some cases have not examined partner identity. We performed targeted sequencing of MYC , BCL2 , BCL6 , and the immunoglobulin ( IG ) loci in 112 tumors with DLBCL morphology harboring MYC rearrangement...
October 23, 2018: Blood Advances
Junguo Ma, Xi Chen, Guangyuan Xin, Xiaoyu Li
The present study aimed to determine the chronic toxicity of 1-methyl-3-octylimidazolium bromide ([C8 mim]Br) on the silver carp to further reveal the toxicological mechanisms of ionic liquids. Chronic exposure of silver carp to [C8 mim]Br at concentrations of 1.095 and 4.380 mg/L for 60 d was conducted under laboratory conditions. The results revealed that chronic exposure to [C8 mim]Br inhibited the activity of superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPx) and reduced glutathione (GSH) levels while markedly increasing malondialdehyde (MDA) and protein carbonyl (PC) levels in fish spleen, indicating that [C8 mim]Br treatment induced oxidative stress...
October 19, 2018: Fish & Shellfish Immunology
Anahita Nosrati, Ahmad Monabati, Alireza Sadeghipour, Fatemeh Radmanesh, Akbar Safaei, Sajjadeh Movahedinia
Primary central nervous system lymphoma (PCNSL) is a rare specific subtype of non-Hodgkin lymphoma limited to the brain, leptomeninges, spinal cord, or eyes without any systemic presentation and relapse which mostly takes place in CNS. In more than 95% of patients, it is of diffuse large B cell lymphoma (DLBCL) type. Categorizing PCNSL to germinal center cell like or activated B cell like, as we usually do for DLBCL NOS, may not be applicable for predicting outcome. Possible prognostic significance of MYC, BCL2, and/or BCL6 rearrangements may be important given what we know about their impact in systemic DLBCL, but we have limited knowledge about the status of double or triple hit molecular changes in PCNSL...
October 11, 2018: Annals of Hematology
Karim Rihawi, Roberta Alfieri, Michelangelo Fiorentino, Francesca Fontana, Elisa Capizzi, Andrea Cavazzoni, Mario Terracciano, Silvia La Monica, Alberto Ferrarini, Genny Buson, Pier Giorgio Petronini, Andrea Ardizzoni
INTRODUCTION: Translocations of the anaplastic lymphoma kinase (ALK) can be effectively targeted in advanced non-small cell lung cancer by ALK-TKI inhibitors including Crizotinib. However, the development of acquired resistance often limits the duration of these therapies. While several mechanisms of secondary resistance have been already identified, little is known about molecular determinants of primary resistance. In our brief report we investigated the tumor molecular profile of a patient who failed to respond to Crizotinib...
October 2, 2018: Translational Oncology
Pekka Peroja, Mette Pedersen, Tuomo Mantere, Peter Nørgaard, Jenni Peltonen, Kirsi-Maria Haapasaari, Jan Böhm, Esa Jantunen, Taina Turpeenniemi-Hujanen, Katrin Rapakko, Peeter Karihtala, Ylermi Soini, Kaija Vasala, Outi Kuittinen
Diffuse large B-cell lymphoma (DLBCL) is an aggressive lymphoma with diverse outcomes. Concurrent translocation of MYC and BCL-2 and/or BCL-6, and concurrent immunohistochemical (IHC) high expression of MYC and BCL-2, have been linked to unfavorable treatment responses. TP53-mutated DLBCL has also been linked to worse outcome. Our aim was to evaluate the aforementioned issues in a cohort of 155 patients uniformly treated with R-CHOP-like therapies. We performed direct sequencing of TP53 exons 5, 6, 7 and 8 as well as fluorescence in-situ hybridization (FISH) of MYC, BCL-2 and BCL-6, and IHC of MYC, BCL-2 and BCL-6...
October 4, 2018: Scientific Reports
Jie Huang, Dongxian Jiang, Ting Zhu, Yanqiu Wang, Hao Wang, Qun Wang, Lijie Tan, Hongguang Zhu, Yingyong Hou, Junxia Yao
BACKGROUND: We investigated the frequency of c-MYC amplification in esophageal squamous cell carcinoma (ESCC), including both stage I-II and III-IVa disease, and evaluated the correlation of c-MYC amplification with clinicopathologic parameters and outcome. METHODS: In 259 ESCC resected at Zhongshan Hospital, Fudan University from January 2007 to November 2010, c-MYC amplification was analyzed using tissue microarray, with fluorescence in situ hybridization (FISH) assay...
September 28, 2018: Annals of Thoracic Surgery
Saihong Yan, Miao Wang, Xuefang Liang, Christopher J Martyniuk, Jinmiao Zha, Zijian Wang
To assess hepatotoxicity and to determine the underlying mechanisms of carbamazepine (CBZ) toxicity in fish, histopathology and the liver proteome were examined after Chinese rare minnow (Gobiocypris rarus) were exposed to 1, 10, and 100 μg/L CBZ for 28 days. Histopathological changes included disruption of spatial structure, pyknotic nuclei, cellular vacuolization and deformation of cell nuclei, in addition to marked swelling of hepatocytes in all treatment groups. Protein analysis revealed that there were gender-specific responses in rare minnow following exposure, and there were 47 proteins in females and 22 proteins in males identified as differentially abundant following CBZ treatments...
December 2018: Environmental Pollution
Asaad Azarnezhad, Mina Tabrizi, Firouzeh Javan, Parvin Mehdipour
Background/aim: The aims of this study were to detect CCND1 , C-MYC , and FGFR1 amplification using qPCR, confirmation with FISH, and to further assess their clinicopathological relevance. Materials and methods: Thirty-five breast tumor samples were analyzed for amplification of the selected genes using modified SYBR Green qPCR. The accuracy of the qPCR was assessed by FISH as a gold-standard method. Results: CCND1 , C-MYC , and FGFR1 amplifications were observed in 34...
August 16, 2018: Turkish Journal of Medical Sciences
Anne M R Schrader, Patty M Jansen, Maarten H Vermeer, Johanna K Kleiverda, Joost S P Vermaat, Rein Willemze
Primary cutaneous diffuse large B-cell lymphoma, leg type (PCDLBCL-LT) and primary cutaneous follicle center lymphoma (PCFCL) are cutaneous B-cell lymphomas (CBCL) with different clinical characteristics and behavior. PCDLBCL-LT is the most aggressive CBCL with a relatively poor prognosis. In nodal diffuse large B-cell lymphoma (DLBCL), rearrangements of the MYC gene, especially in combination with a second hit in BCL2 and/or BCL6, and double protein expression of MYC and BCL2 (DE) are adverse prognostic factors...
November 2018: American Journal of Surgical Pathology
Chiara Borga, Gilseung Park, Clay Foster, Jessica Burroughs-Garcia, Matteo Marchesin, Rikin Shah, Ameera Hasan, Syed T Ahmed, Silvia Bresolin, Lance Batchelor, Teresa Scordino, Rodney R Miles, Geertruy Te Kronnie, James L Regens, J Kimble Frazer
Precursor-B cell acute lymphoblastic leukemia (pre-B ALL) is the most common pediatric cancer, but there are no useful zebrafish pre-B ALL models. We describe the first highly- penetrant zebrafish pre-B ALL, driven by human MYC. Leukemias express B lymphoblast-specific genes and are distinct from T cell ALL (T-ALL)-which these fish also develop. Zebrafish pre-B ALL shares in vivo features and expression profiles with human pre-B ALL, and these profiles differ from zebrafish T-ALL or normal B and T cells. These animals also exhibit aberrant lymphocyte development...
August 15, 2018: Leukemia: Official Journal of the Leukemia Society of America, Leukemia Research Fund, U.K
Fuli Yao, Licheng Yin, Shiyu Feng, Xinyan Wang, Anying Zhang, Hong Zhou
In mammals, both runt-related transcription factor 3 (RUNX3) and c-Myc are the downstream effectors of transforming growth factor-β1 (TGF-β1) signaling to mediate various cellular responses. However, information of their interaction especially in fish is lacking. In the present study, grass carp (Ctenopharyngodon idella) runx3 (gcrunx3) cDNA was cloned and identified. Interestingly, opposing effects of recombinant grass carp TGF-β1 (rgcTGF-β1) on c-myc and runx3 mRNA expression were observed in grass carp periphery blood lymphocytes (PBLs)...
August 10, 2018: Fish & Shellfish Immunology
Tarek Eldessouki, Kelly Hanley, Fatima Hamadeh, Olaronke O Oshilaja, Charles D Sturgis
The Revised fourth Edition World Health Organization (WHO) Classification of Tumors of Hematopoietic and Lymphoid Tissues suggests novel categories, including "high grade B-cell lymphoma with MYC and BCL2 and BCL6 gene rearrangements." These diseases are known colloquially as "double hit" and "triple hit" lymphomas. The "first-hit" in these cases is the harboring of a MYC rearrangement. Concurrent derangements of BCL2 and BCL6 can be the "second-hit" or "third-hit...
September 2018: Diagnostic Cytopathology
Mikiko Ise, Hajime Kageyama, Dai Ikebe, Akinobu Araki, Kyoya Kumagai, Makiko Itami
Follicular lymphoma (FL) is genetically characterized by BCL2/IGH translocation. Some FL cases histologically transform to high-grade lymphoma, and the majority of cases transform to diffuse large B-cell lymphoma. We report herein an unusual FL case that transformed to plasmablastic lymphoma (PBL) with MYC gene rearrangement as early as 12 months after FL diagnosis. IGH/MYC translocation, the most common cytogenetic abnormality seen in de novo PBL, was also detected in the transformed tumor (double-hit lymphoma)...
September 19, 2018: Journal of Clinical and Experimental Hematopathology: JCEH
Shaoying Li, Pei Lin, L Jeffrey Medeiros
The designation high-grade B-cell lymphoma (HGBL) has been incorporated into the 2016 Revision of the WHO classification of lymphoid neoplasms and includes two types: (1) HGBL, not otherwise specified; and (2) HGBL with MYC and BCL2 and/or BCL6 rearrangements, also known as double or triple hit lymphoma (DHL/THL). These categories of lymphomas represent 1-2% of non-Hodgkin lymphomas and a considerable portion of DLBCL patients who are primary refractory to R-CHOP therapy. It corresponds to the designation 'B-cell lymphoma, unclassifiable, with features intermediate between DLBCL and Burkitt lymphoma' in the 2008 WHO classification...
August 2018: Expert Review of Hematology
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