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Lina Marcela Barrera, León Darío Ortiz, Hugo Grisales, Mauricio Rojas, Mauricio Camargo
Introduction: The quantification of chromosomal instability is an important parameter to assess genotoxicity and radiosensitivity. Most conventional techniques require cell cultures or laborious microscopic analyses of chromosomes or nuclei. However, a flow cytometry that selects the reticulocytes has been developed as an alternative for in vivo studies, which expedites the analytical procedures and increases up to 20 times the number of target cells to be analyzed. Objectives: To standardize the flow cytometry parameters for selecting and quantifying the micronucleated reticulocytesCD71+ (MN-RET) from freshly drawn peripheral blood and to quantify the frequency of this abnormal cell subpopulation as a measure of cytogenetic instability in populations of healthy volunteers (n =25), and patients (n=25), recently diagnosed with high-grade gliomas before the onset of treatment...
September 1, 2018: Biomédica: Revista del Instituto Nacional de Salud
Saad O Atiq, Osman O Atiq, Mohammad O Atiq, Kara C Phillips, Blake B Jacks, Mauricio Moreno, Sanjay Maraboyina, Omar T Atiq
BACKGROUND Cancer is the second leading cause of death internationally, resulting in millions of deaths each year. While treatment in the past has heavily relied on surgery and radiotherapy, chemotherapy and immunotherapy are being increasingly utilized depending on disease presentation. CASE REPORT A 56-year-old male presented to the Emergency Department with a 3-week history of a rapidly enlarging left supraclavicular neck mass. Computed tomography scan revealed a 12×13 cm mass extending from the angle of the mandible to the supraclavicular area...
October 18, 2018: American Journal of Case Reports
Renato M Passos, José Belucio-Neto, Camilla O Xavier, Eduardo A Novais, Mauricio Maia, Michel Eid Farah
OBJECTIVE: To compare two different laser strategies of panretinal photocoagulation for diabetic retinopathy. METHODS: Single-center, randomized study including 41 eyes treated with 577-nm multispot laser with a 20-ms pulse duration (group 1) or a 532-nm single-spot laser with a 100-ms pulse duration (group 2). The outcomes included best-corrected visual acuity (BCVA) and imaging changes at baseline, 6 and 12 months, laser parameters, and results of subjective pain analysis...
October 17, 2018: Ophthalmologica. Journal International D'ophtalmologie
Mauricio Yugo de Souza, Rebeca DI Nicoló, Eduardo Bresciani
The aim of this study was to evaluate the effect of ethanol on the bond longevity of a universal adhesive system to bovine dentin, under different modes of adhesive application and artificial aging. Bovine dentin was exposed, and the smear layer was standardized by sandpaper polishing. Specimens were randomly divided into 2 groups: ethanol (E) and non-ethanol (N). Groups were subdivided according to adhesive mode of application into etch-and-rinse (Er) and self-etching (S). Resin blocks were built onto the treated surface, and the specimens were stored in deionized water at 37°C for 48 h...
October 11, 2018: Brazilian Oral Research
Vivianne Chappuis, Gustavo Avila-Ortiz, Mauricio G Araújo, Alberto Monje
OBJECTIVES: The aim of this systematic review was to investigate the association between the intake of systemic medications that may affect bone metabolism and their subsequent impact on implant failures. MATERIAL AND METHODS: Electronic and manual literature searches were conducted. Implant failure (IF) was the primary outcome, while biological/mechanical and the causes/timing associated with IF were set as secondary outcomes. Meta-analyses for the binary outcome IF and odds ratio were performed to investigate the association with medications...
October 2018: Clinical Oral Implants Research
Ronald E Jung, Bilal Al-Nawas, Mauricio Araujo, Gustavo Avila-Ortiz, Stephen Barter, Nadine Brodala, Vivianne Chappuis, Bo Chen, Andre De Souza, Ricardo Faria Almeida, Stefan Fickl, Gary Finelle, Jeffrey Ganeles, Hadi Gholami, Christoph Hammerle, Simon Jensen, Asbjørn Jokstad, Hideaki Katsuyama, Johannes Kleinheinz, Chatchai Kunavisarut, Nikos Mardas, Alberto Monje, Panos Papaspyridakos, Michael Payer, Eik Schiegnitz, Ralf Smeets, Martina Stefanini, Christiaan Ten Bruggenkate, Konstantinos Vazouras, Hans-Peter Weber, Dieter Weingart, Péter Windisch
OBJECTIVES: The aim of Working Group 1 was to address the influence of different local (implant length, diameter, and design) and systemic (medications) factors on clinical, radiographic, and patient-reported outcomes in implant dentistry. Focused questions on (a) short posterior dental implants (≤6 mm), (b) narrow diameter implants, (c) implant design (tapered compared to a non-tapered implant design), and (d) medication-related dental implant failures were addressed. MATERIALS AND METHODS: Four systematic reviews were prepared in advance of the Consensus Conference and were discussed among the participants of Group 1...
October 2018: Clinical Oral Implants Research
Lisa J Heitz-Mayfield, Merete Aaboe, Mauricio Araujo, Juan B Carrión, Raffaele Cavalcanti, Norbert Cionca, David Cochran, Ivan Darby, Eiji Funakoshi, Petra C Gierthmuehlen, Dena Hashim, Leila Jahangiri, Yongdae Kwon, France Lambert, Danielle M Layton, Eduardo R Lorenzana, Gerald McKenna, Andrea Mombelli, Frauke Müller, Mario Roccuzzo, Giovanni E Salvi, Martin Schimmel, Murali Srinivasan, Cristiano Tomasi, Alvin Yeo
OBJECTIVES: The aim of Working Group 4 was to address topics related to biologic risks and complications associated with implant dentistry. Focused questions on (a) diagnosis of peri-implantitis, (b) complications associated with implants in augmented sites, (c) outcomes following treatment of peri-implantitis, and (d) implant therapy in geriatric patients and/or patients with systemic diseases were addressed. MATERIALS AND METHODS: Four systematic reviews formed the basis for discussion in Group 4...
October 2018: Clinical Oral Implants Research
Bernal Stewart, Jamil Awad Shibli, Mauricio Araujo, Luciene Cristina Figueiredo, Fotinos Panagakos, Flavia Matarazzo, Renata Mairink, Tatiana Onuma, Marcelo Faveri, Belén Retamal-Valdes, Magda Feres
OBJECTIVE: To evaluate the effects of a toothpaste containing 0.3% triclosan in volunteers that have been treated for peri-implantitis and were enrolled in a maintenance program. MATERIAL AND METHODS: Subjects presenting at least one implant with peri-implantitis were selected. They received anti-infective surgical therapy, and sixty days post-surgery (baseline) were randomized into two groups, brushing twice/day for 2 years (a) with a toothpaste containing 0.3% triclosan+2...
October 2018: Clinical Oral Implants Research
Aileen L Rowland, Cristobal Navas de Solis, Mauricio A Lepiz, Kevin J Cummings, Ashlee E Watts
Bone marrow is commonly collected from horses for regenerative medicine applications. Little information is available regarding pain experienced by the horse during bone marrow aspiration. The objective of this study was to characterize horse reaction and pain response during bone marrow aspiration (BMA) compared to a sham (SHAM) procedure. We hypothesized there would be significantly greater horse reaction or pain response measured by salivary cortisol, heart rate variability, and depth and duration of sedation between BMA and SHAM...
2018: Frontiers in Veterinary Science
Diana Medeiros Andrade, Gabriela Fernandes, Renato Miranda, Danilo Reis Coimbra, Maurício G Bara Filho
Andrade, DM, Fernandes, G, Miranda, R, Reis Coimbra, D, and Bara Filho, MG. Training load and recovery in volleyball during a competitive season. J Strength Cond Res XX(X): 000-000, 2018-The objective of this study was to analyze the training load (TL) and recovery status (RS) of a volleyball team in different periods of the season. Fifteen male athletes participated in the study. Training load was quantified through the rate of perceived exertion of session. The total weekly TL of the preparatory period (PP), competitive period I (CPI), and competitive period II (CPII) was calculated...
October 12, 2018: Journal of Strength and Conditioning Research
Rafael F Coelho, Mauricio D Cordeiro, Guilherme P Padovani, Rafael Localli, Limirio Fonseca, José Pontes, Giuliano B Guglielmetti, Miguel Srougi, William Carlos Nahas
OBJECTIVE: To evaluate the length hospital stay and predictors of prolonged hospitalization after RRP performed in a high-surgical volume teaching institution, and analyze the rate of unplanned visits to the office, emergency care, hospital readmissions and perioperative complications rates. MATERIALS AND METHODS: Retrospective analysis of prospectively collected data in a standardized database for patients with localized prostate cancer undergoing RRP in our institution between January/2010 - January/2012...
September 10, 2018: International Braz J Urol: Official Journal of the Brazilian Society of Urology
Luna Liu, Keyang Xu, Jenny Li, Mauricio Maia, Mary Mathieu, Rebecca Elliott, Jihong Yang, Ihsan Nijem, Surinder Kaur
BACKGROUND: Hybrid ligand-binding (LB) LC-MS/MS protein quantitative assays involve a LB step for analyte enrichment that has less stringent requirements than the conventional LB assays. RESULTS: Herceptin™(trastuzumab) binding to HER2 extracellular domain was evaluated using on-bead and off-bead capture formats. The two formats yielded significantly different trastuzumab concentrations in human and monkey serum pharmacokinetic samples. Biotransformations, including deamidation of asparagine and isomerization of aspartic acid near the complementarity-determining regions of trastuzumab, had a profound impact on the LB step for analyte enrichment and trastuzumab quantification...
October 16, 2018: Bioanalysis
Nathan Y Yu, Mauricio E Gamez
No abstract text is available yet for this article.
September 17, 2018: International Journal of Surgical Pathology
Samuel Novais, Nancy Calderón-Cortés, Gumersindo Sánchez-Montoya, Mauricio Quesada
The twig-girdler beetle Oncideres albomarginata chamela (Chemsak and Giesbert) (Cerambycidae: Lamiinae) detaches branches of Spondias purpurea L. (Sapindales: Anacardiaceae) that fall on the forest floor or remain suspended on vegetation. Many wood-boring beetles also oviposit in these branches and larval development creates cavities that are abandoned when the adults emerge. The objective of this study was to evaluate the role of wood-boring beetles as facilitators by creating new habitats for arthropods, and test for vertical stratification and temporal variation of arthropods associated with S...
September 1, 2018: Journal of Insect Science
Mauricio Castro-Sepulveda, Rodrigo Ramirez-Campillo, Felipe Abad-Colil, Camila Monje, Luis Peñailillo, Jorge Cancino, Hermann Zbinden-Foncea
A soccer match induce changes in physiological stress biomarkers as testosterone (T), cortisol (C), and testosterone:cortisol (T:C) ration. Hydration state may also modulate these hormones, and therefore may alter the anabolic/catabolic balance in response to soccer match. The role of hydration status before the match in this biomarkers has not yet been reported. The aim of this study was to compare the salivary T, C, and the T:C ratio responses after two friendly matches in well-hydrated and mild-dehydrated (MD) elite young male soccer player...
2018: Frontiers in Physiology
Aimé Rubini Pisano, Marcela Moré, Mauricio A Cisternas, Robert A Raguso, Santiago Benitez-Vieyra
Phenotypic intermediacy is an indicator of putative hybrid origin, and has provided the main clues to discovering hybrid plants in nature. Mandevilla pentlandiana and M. laxa (Apocynaceae) are sister species with clear differences in floral phenotype and associated pollinator guilds: diurnal Hymenoptera and nocturnal hawkmoths, respectively. The presence of individuals with intermediate phenotypes in a wild population raises questions about the roles of visual and olfactory signals (i.e. corolla morphology and floral fragrances) as barriers to interbreeding, and how the breakdown of floral isolation occurs...
October 14, 2018: Plant Biology
Carolina Gonzales da Silva, Carlos Frederico Martins, Heidi Christina Bessler, Álvaro Moraes da Fonseca Neto, Tereza Cristina Cardoso, Maurício Machaim Franco, Anelise Dos Santos Mendonça, Ligiane de Oliveira Leme, José Renato Junqueira Borges, Juaci Vitoria Malaquias, Sônia Nair Báo
The aim of this work was investigate the methylation and hydroximethylation status of mesenchymal stem cells (MSC) from amniotic fluid (MSC-AF), adipose tissue (MSC-AT) and fibroblasts (FIB-control) and verify the effect of trichostatin A (TSA) on gene expression and development of cloned bovine embryos produced using these cells. Characterization of MSC from two animals (BOV1 and BOV2) was performed by flow cytometry, immunophenotyping and analysis of cellular differentiation genes expression. The cells were used in the nuclear transfer in the absence or presence of 50 nM TSA for 20 h in embryo culture...
October 14, 2018: Reproduction in Domestic Animals, Zuchthygiene
Laurence Culot, Lucas Augusto Pereira, Ilaria Agostini, Marco Antônio Barreto de Almeida, Rafael Souza Cruz Alves, Izar Aximoff, Alex Bager, María Celia Baldovino, Thiago Ribas Bella, Júlio César Bicca-Marques, Caryne Braga, Carlos Rodrigo Brocardo, Ana Kellen Nogueira Campelo, Gustavo R Canale, Jader da Cruz Cardoso, Eduardo Carrano, Diogo Cavenague Casanova, Camila Righetto Cassano, Erika Castro, Jorge José Cherem, Adriano Garcia Chiarello, Braz Antonio Pereira Cosenza, Rodrigo Costa-Araújo, Nilmara Cristina da Silva, Mario S Di Bitetti, Aluane Silva Ferreira, Priscila Coutinho Ribas Ferreira, Marcos de S Fialho, Lisieux Franco Fuzessy, Guilherme Siniciato Terra Garbino, Francini de Oliveira Garcia, Cassiano A F R Gatto, Carla Cristina Gestich, Pablo Rodrigues Gonçalves, Nila Rássia Costa Gontijo, Maurício Eduardo Graipel, Carlos Eduardo Guidorizzi, Robson Odeli Espíndola Hack, Gabriela Pacheco Hass, Renato Richard Hilário, André Hirsch, Ingrid Holzmann, Daniel Henrique Homem, Hilton Entringer Júnior, Gilberto Sabino-Santos Júnior, Maria Cecília Martins Kierulff, Christoph Knogge, Fernando Lima, Elson Fernandes de Lima, Cristiana Saddy Martins, Adriana Almeida de Lima, Alexandre Martins, Waldney Pereira Martins, Fabiano R de Melo, Ricardo Melzew, João Marcelo Deliberador Miranda, Flávia Miranda, Andréia Magro Moraes, Tainah Cruz Moreira, Maria Santina de Castro Morini, Mariana B Nagy-Reis, Luciana Oklander, Leonardo de Carvalho Oliveira, Adriano Pereira Paglia, Anderson Pagoto, Marcelo Passamani, Fernando de Camargo Passos, Carlos A Peres, Michell Soares de Campos Perine, Míriam Plaza Pinto, Antonio Rossano Mendes Pontes, Marcio Port Carvalho, Bárbara Heliodora Soares do Prado, André Luis Regolin, Gabriela Cabral Rezende, Alessandro Rocha, Joedison Dos S Rocha, Raisa Reis de Paula Rodarte, Lilian Patrícia Sales, Edmilson Dos Santos, Paloma Marques Santos, Christine Steiner São Bernardo, Ricardo Sartorello, Leonardo La Serra, Eleonore Setz, Anne Sophie de Almeida E Silva, Leonardo Henrique da Silva, Pedro Bencke Ermel da Silva, Maurício Silveira, Rebecca L Smith, Sara Machado de Souza, Ana Carolina Srbek-Araujo, Leonardo Carreira Trevelin, Claudio Valladares Padua, Luciana Zago, Eduardo Marques, Stephen Francis Ferrari, Raone Beltrão-Mendes, Denison José Henz, Francys E da Veiga da Costa, Igor Kintopp Ribeiro, Lucas Lacerda Toth Quintilham, Marcos Dums, Pryscilla Moura Lombardi, Renata Twardowsky Ramalho Bonikowski, Stéfani Gabrieli Age, João Pedro Souza-Alves, Renata Chagas, Rogério Grassetto Teixeira da Cunha, Monica Mafra Valença-Montenegro, Gabriela Ludwig, Leandro Jerusalinsky, Gerson Buss, Renata Bocorny de Azevedo, Roberio Freire Filho, Felipe Bufalo, Louis Milhe, Mayara Mulato Dos Santos, Raíssa Sepulvida, Daniel da Silva Ferraz, Michel Barros Faria, Milton Cezar Ribeiro, Mauro Galetti
Primates play an important role in ecosystem functioning and offer critical insights into human evolution, biology, behavior, and emerging infectious diseases. There are 26 primate species in the Atlantic Forests of South America, 19 of them endemic. We compiled a dataset of 5,472 georeferenced locations of 26 native and 1 introduced primate species, as hybrids in the genera Callithrix and Alouatta. The dataset includes 700 primate communities, 8,121 single species occurrences and 714 estimates of primate population sizes, covering most natural forest types of the tropical and subtropical Atlantic Forest of Brazil, Paraguay and Argentina and some other biomes...
October 13, 2018: Ecology
Fernanda Alves de Freitas Guedes, Priscilla de Barros Rossetto, Fábia Guimarães, Maurício Wolf Wilwerth, Jorge Eduardo Santos Paes, Marisa Fabiana Nicolás, Fernanda Reinert, Raquel Soares Peixoto, Márcio Alves-Ferreira
Mangroves are ecosystems of economic and ecological importance. Laguncularia racemosa (Combretaceae), popularly known as white mangrove, is a species that greatly contributes to the community structure of neotropical and West African mangrove forests. Despite the significance of these ecosystems, they have been destroyed by oil spills that can cause yellowing of leaves, increased sensitivity to other stresses and death of trees. However, the molecular response of plants to oil stress is poorly known. In this work, Illumina reads were de novo assembled into 46,944 transcripts of L...
September 7, 2018: Aquatic Toxicology
Márcia Marques Silveira, Henrique Xavier Salgado Bayão, Anelise Dos Santos Mendonça, Naiara Araújo Borges, Luna Nascimento Vargas, Alexandre Rodrigues Caetano, Rodolfo Rumpf, Maurício Machaim Franco
INTRODUCTION: Cloning via somatic cell nuclear transfer (SCNT) has been associated with a variety of pathologies, primarily in the placenta, and these alterations may be associated with aberrant epigenetic reprogramming of the donor cell genome. We tested the hypothesis that DNA methylation patterns are not appropriately established after nuclear transfer and that those altered patterns are associated with specific aberrant phenotypes. METHODS: We compared global and specific placental DNA methylation patterns between aberrant and healthy SCNT-produced calves...
October 2018: Placenta
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