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https://www.readbyqxmd.com/read/30338855/a-bap31-intrabody-induces-gastric-cancer-cell-death-by-inhibiting-p27-kip1-proteasome-degradation
#1
Jing Chen, Haotian Guo, Haitao Jiang, Mwichie Namusamba, Changli Wang, Tian Lan, Tianyi Wang, Bing Wang
B-cell receptor-associated protein 31 (BAP31) is a ubiquitously expressed endoplasmic reticulum (ER) membrane protein that has been found to be overexpressed in gastric intestinal-type adenocarcinoma. We first studied the relationship of BAP31 with 84 kinds of tumor-associated antigens and found that BAP31 can specifically interact with and regulate the proteasome degradation of the cyclin kinase inhibitor p27kip1 , which is one of the most frequently dysregulated tumor suppressor proteins in human cancers...
October 19, 2018: International Journal of Cancer. Journal International du Cancer
https://www.readbyqxmd.com/read/30324117/selection-and-evaluation-of-specific-single-chain-antibodies-against-cd90-a-marker-for-mesenchymal-and-cancer-stem-cells
#2
Bahareh Moazen, Amir Zarrinhaghighi, Foroogh Nejatollahi
Background: CD90, a membrane-associated glycoprotein is a marker used to identify mesenchymal stem cells (MSCs). Recent studies have introduced CD90, which induces tumorigenic activity, as a cancer stem cell (CSC) marker in various malignancies. Blocking CD90 activity with anti-CD90 monoclonal antibodies enhanced anti-tumor effects. To date, highly specific antibody single-chain variable fragments (scFvs) have been isolated against various targets and showed promising results in cancer immunotherapy...
October 2018: Reports of Biochemistry & Molecular Biology
https://www.readbyqxmd.com/read/30321407/immunodiagnostic-potential-of-wuchereria-bancrofti-l1-antigen-based-filarial-immunoglobulin-g4-detection-assay
#3
B V Siva Prasad, Vishal Khatri, P Suresh Yadav, M Subhosh Chandra, D Vijaya Lakshmi, Kalyan Goswami
Background: After mass drug administration to eliminate human lymphatic filariasis, there is a need for surveillance to detect the measurable endpoint of the program. Methods: An immunodominant seroreactive clone, WbL1, was identified through immunoscreening of a Wuchereria bancrofti L3 complementary DNA expression library. Recombinant WbL1 (rWbL1) was analysed with sera from W. bancrofti patients. Diagnostic evaluation was carried out by developing an enzyme-linked immunosorbent assay (ELISA) to detect the filarial-specific antibodies in various categories of filarial sera samples against recombinant WbL1 (rWbL1) protein...
October 15, 2018: Transactions of the Royal Society of Tropical Medicine and Hygiene
https://www.readbyqxmd.com/read/30320250/reporter-selection-for-nanotags-in-multiplexed-surface-enhanced-raman-spectroscopy-assays
#4
Maria Sánchez-Purrà, Biel Roig-Solvas, Cristina Rodriguez-Quijada, Brianna M Leonardo, Kimberly Hamad-Schifferli
We report a quantitative evaluation of the choice of reporters for multiplexed surface-enhanced Raman spectroscopy (SERS). An initial library consisted of 15 reporter molecules that included commonly used Raman dyes, thiolated reporters, and other small molecules. We used a correlation matrix to downselect Raman reporters from the library to choose five candidates: 1,2-bis(4-pyridyl)ethylene, 4-mercaptobenzoic acid, 3,5-dichlorobenzenthiol, pentachlorothiophenol, and 5,5'-dithiobis(2-nitrobenzoic acid). We evaluated the ability to distinguish the five SERS reporters in a dipstick immunoassay for the biomarker human IgG...
September 30, 2018: ACS Omega
https://www.readbyqxmd.com/read/30303958/novel-rna-aptamers-targeting-gastrointestinal-cancer-biomarkers-cea-ca50-and-ca72-4-with-superior-affinity-and-specificity
#5
Qing Pan, Carmen O K Law, Mingo M H Yung, K C Han, Yuen Lam Pon, Terrence Chi Kong Lau
Gastric cancer is the third most common cause of death from cancer in the world and it remains difficult to cure in Western countries, primarily because most patients present with advanced disease. Currently, CEA, CA50 and CA72-4 are commonly used as tumor markers for gastric cancer by immunoassays. However, the drawback and conundrum of immunoassay are the unceasing problem in standardization of quality of antibodies and time/effort for the intensive production. Therefore, there is an urgent need for the development of a standardized assay to detect gastric cancer at the early stage...
2018: PloS One
https://www.readbyqxmd.com/read/30302282/as03-adjuvanted-h5n1-vaccine-promotes-antibody-diversity-and-affinity-maturation-nai-titers-cross-clade-h5n1-neutralization-but-not-h1n1-cross-subtype-neutralization
#6
Surender Khurana, Elizabeth M Coyle, Jody Manischewitz, Lisa R King, Jin Gao, Ronald N Germain, Pamela L Schwartzberg, John S Tsang, Hana Golding
Immune responses to inactivated vaccines against avian influenza are poor due in part to lack of immune memory. Adjuvants significantly increased virus neutralizing titers. We performed comprehensive analyses of polyclonal antibody responses following FDA-approved adjuvanted H5N1-A/Indonesia vaccine, administered in presence or absence of AS03. Using Whole Genome Fragment Phage Display Libraries, we observed that AS03 induced antibody epitope diversity to viral hemagglutinin (HA) and neuraminidase compared with unadjuvanted vaccine...
2018: NPJ Vaccines
https://www.readbyqxmd.com/read/30301315/generation-and-characterization-of-a-neutralizing-human-monoclonal-antibody-to-hepatitis-b-virus-pres1-from-a-phage-displayed-human-synthetic-fab-library
#7
Gyunghee Jo, Mun Sik Jeong, Jimin Wi, Doo Hyun Kim, Sangkyu Kim, Dain Kim, Jun-Yeol Yoon, Heesu Chae, Kyun-Hwan Kim, Hyo Jeong Hong
The hepatitis B virus (HBV) envelope contains small (S), middle (M), and large (L) proteins. PreS1 of the L protein contains a receptor-binding motif crucial for HBV infection. This motif is highly conserved among 10 HBV genotypes (A-J), making it a potential target for the prevention of HBV infection. In this study, we successfully generated a neutralizing human monoclonal antibody (mAb), 1A8 (IgG1), that recognizes the receptor-binding motif of preS1 using a phage-displayed human synthetic Fab library. Analysis of the antigen-binding activity of 1A8 for different genotypes indicated that it can specifically bind to the preS1 of major HBV genotypes (A-D)...
August 28, 2018: Journal of Microbiology and Biotechnology
https://www.readbyqxmd.com/read/30296945/should-anti-egfr-mabs-be-discontinued-for-conversion-surgery-in-untreated-right-sided-metastatic-colorectal-cancer-a-systematic-review-and-meta-analysis
#8
REVIEW
Datian Chen, Xiang Zhang, Guangyi Gao, Lili Shen, Jiaqi Xie, Xiaoping Qian, Huiyu Wang
BACKGROUND: Previous studies have demonstrated that left-sided tumors have better prognoses than right-sided tumors in RAS wild-type mCRC (metastatic colorectal cancer) patients, while anti-EGFR mAbs appear to have no advantage compared with bevacizumab for right-sided tumors in these patients. Nevertheless, it remains unclear whether primary tumor location affects patients' options for potentially curative resection. METHODS: PubMed, the Cochrane Library, Embase, ASCO, and ESMO conference abstracts were searched...
October 8, 2018: World Journal of Surgical Oncology
https://www.readbyqxmd.com/read/30294976/available-and-future-treatments-for-atypical-parkinsonism-a-systematic-review
#9
REVIEW
Davide Vito Moretti
AIMS: Success in treating patients with atypical parkinsonian syndromes, namely progressive supranuclear palsy (PSP), cortico-basal degeneration (CBD), multiple system atrophy (MSA), Parkinson's disease with dementia (PDD), and Lewy body dementia with (LBD), remains exceedingly low. The present work overviews the most influential research literature collected on MEDLINE, ISI Web of Science, Cochrane Library, and Scopus for available treatment in atypical parkinsonisms without time restriction...
October 7, 2018: CNS Neuroscience & Therapeutics
https://www.readbyqxmd.com/read/30293984/long-range-pcr-libraries-and-next-generation-sequencing-for-pharmacogenetic-studies-of-patients-treated-with-anti-tnf-drugs
#10
Michal Walczak, Marzena Skrzypczak-Zielinska, Marianna Plucinska, Oliwia Zakerska-Banaszak, Daria Marszalek, Liliana Lykowska-Szuber, Kamila Stawczyk-Eder, Agnieszka Dobrowolska, Ryszard Slomski
Biological therapy with anti-tumor necrosis factor-α (anti-TNF-α) monoclonal antibodies significantly increased the effectiveness of autoimmune disease treatment compared with conventional medicines. However, anti-TNF-α drugs are relatively expensive and a response to the therapy is reported in only 60-70% of patients. Moreover, in up to 5% of patients adverse drug reactions occur. The various effects of biological treatment may be a potential consequence of interindividual genetic variability. Only a few studies have been conducted in this field and which refer to single gene loci...
October 8, 2018: Pharmacogenomics Journal
https://www.readbyqxmd.com/read/30291985/transcriptomic-analysis-of-immunoglobulin-novel-antigen-receptor-ignar-heavy-chain-constant-domains-of-brownbanded-bamboo-shark-chiloscyllium-punctatum
#11
D P N De Silva, E Tan, N Mizuno, S Hosoya, Md S Reza, S Watabe, S Kinoshita, S Asakawa
Cartilaginous fish are the evolutionarily oldest group of animals which possess antibodies, T cell receptors and major histocompatibility complex (MHC). The immunoglobulin novel antigen receptor (IgNAR) found in cartilaginous fish is a heavy chain homodimer which lacks light chain. The presence of non-canonical cysteine molecules and lack of CDR2 region make it more significant. To synthesize active binding domains based on variable region of IgNAR (VNAR), knowledge on the constant region dynamics play a significant role...
October 4, 2018: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/30289692/discovery-and-characterization-of-a-thioesterase-specific-monoclonal-antibody-that-recognizes-the-6-deoxyerythronolide-b-synthase
#12
Xiuyuan Li, Natalia Sevillano, Florencia La Greca, Jake Hsu, Irimpan I Mathews, Tsutomu Matsui, Charles S Craik, Chaitan Khosla
Assembly line polyketide synthases (PKSs) are large multimodular enzymes responsible for the biosynthesis of diverse antibiotics in bacteria. Structural and mechanistic analysis of these megasynthases can benefit from the discovery of reagents that recognize individual domains or linkers in a site-specific manner. Monoclonal antibodies not only have proven themselves as premier tools in analogous applications but also have the added benefit of constraining the conformational flexibility of their targets in unpredictable but often useful ways...
October 17, 2018: Biochemistry
https://www.readbyqxmd.com/read/30287608/impact-of-pcsk9-monoclonal-antibodies-on-circulating-hs-crp-levels-a-systematic-review-and-meta-analysis-of-randomised-controlled-trials
#13
Ye-Xuan Cao, Sha Li, Hui-Hui Liu, Jian-Jun Li
OBJECTIVE: To evaluate the potential effects of proprotein convertase subtilisin/kexin type 9 monoclonal antibody (PCSK9-mAb) on high-sensitivity C reactive protein (hs-CRP) concentrations. DESIGN: A systematic review and meta-analysis of randomised controlled trials. DATA SOURCES: PubMed, MEDLINE, the Cochrane Library databases, ClinicalTrials.gov and recent conferences were searched from inception to May 2018. ELIGIBILITY CRITERIA FOR SELECTING STUDIES: All randomised controlled trials that reported changes of hs-CRP were included...
October 4, 2018: BMJ Open
https://www.readbyqxmd.com/read/30285849/an-expanded-global-inventory-of-allelic-variation-in-the-most-extremely-polymorphic-region-of-plasmodium-falciparum-merozoite-surface-protein-1-provided-by-short-read-sequence-data
#14
Harvey Aspeling-Jones, David J Conway
BACKGROUND: Within Plasmodium falciparum merozoite surface protein 1 (MSP1), the N-terminal block 2 region is a highly polymorphic target of naturally acquired antibody responses. The antigenic diversity is determined by complex repeat sequences as well as non-repeat sequences, grouping into three major allelic types that appear to be maintained within populations by natural selection. Within these major types, many distinct allelic sequences have been described in different studies, but the extent and significance of the diversity remains unresolved...
October 1, 2018: Malaria Journal
https://www.readbyqxmd.com/read/30282451/a-phage-display-identified-peptide-selectively-binds-to-kidney-injury-molecule-1-kim-1-and-detects-kim-1-overexpressing-tumors-in-vivo
#15
Md Enamul Haque, Fatima Khan, Lianhua Chi, Smriti Gurung, Sri Murugan Poongkavithai Vadevoo, Rang-Woon Park, Dong-Kyu Kim, Sang Kyoon Kim, Byungheon Lee
Purpose: This study was carried out to identify a peptide that selectively binds to kidney injury molecule-1 (KIM-1) by screening a phage-displayed peptide library and to use the peptide for the detection of KIM-1overexpressing tumors in vivo. Materials and Methods: Biopanning of a phage-displayed peptide library was performed on KIM-1-coated plates. The binding of phage clones, peptides, and a peptide multimer to the KIM-1 protein and KIM-1‒overexpressing and KIM-1‒low expressing cells was examined by enzyme-linked immunosorbent assay, fluorometry, and flow cytometry...
October 1, 2018: Cancer Research and Treatment: Official Journal of Korean Cancer Association
https://www.readbyqxmd.com/read/30276218/osu-t315-as-an-interesting-lead-molecule-for-novel-b-cell-specific-therapeutics
#16
Kristien Van Belle, Jean Herman, Mark Waer, Ben Sprangers, Thierry Louat
B cells are pathogenic in various disease processes and therefore represent an interesting target for the development of novel immunosuppressants. In the search for new therapeutic molecules, we utilized an in vitro B cell activation assay with ODN2006-stimulated Namalwa cells to screen a chemical library of small molecules for B cell modulating effects. OSU-T315, described as an inhibitor of integrin-linked kinase (ILK), was hereby identified as a hit. On human and murine primary B cells, OSU-T315 potently suppressed the proliferation and the production of antibodies and cytokines upon stimulation, suggesting that ILK could be a promising target in the modulation of B cell activity...
2018: Journal of Immunology Research
https://www.readbyqxmd.com/read/30271892/recombinant-human-b-cell-repertoires-enable-screening-for-rare-specific-and-natively-paired-antibodies
#17
Saravanan Rajan, Michael R Kierny, Andrew Mercer, Jincheng Wu, Andrey Tovchigrechko, Herren Wu, William F Dall Acqua, Xiaodong Xiao, Partha S Chowdhury
The human antibody repertoire is increasingly being recognized as a valuable source of therapeutic grade antibodies. However, methods for mining primary antibody-expressing B cells are limited in their ability to rapidly isolate rare and antigen-specific binders. Here we show the encapsulation of two million primary B cells into picoliter-sized droplets, where their cognate V genes are fused in-frame to form a library of scFv cassettes. We used this approach to construct natively paired phage-display libraries from healthy donors and drove selection towards cross-reactive antibodies targeting influenza hemagglutinin...
2018: Communications biology
https://www.readbyqxmd.com/read/30270696/anti-citrullinated-protein-antibodies-and-arthritis-in-sj%C3%A3-gren-s-syndrome-a-systematic-review-and-meta-analysis
#18
N Molano-González, E Olivares-Martínez, J M Anaya, G Hernández-Molina
OBJECTIVE: The presence of anti-citrullinated protein antibodies (ACPAs) in primary Sjögren's syndrome (pSS) ranges from 3% to 9.9%; however, there is no agreement about their clinical significance. Our aim was to systematically review the literature regarding the association of arthritis and ACPAs in pSS and their role in the development of rheumatoid arthritis (RA). METHOD: A comprehensive search of MEDLINE, ISI Web of Knowledge, and Cochrane Library from inception until June 2016 was undertaken using the combination of two or three of the keywords: primary Sjögren's syndrome, Sjögren's syndrome, arthritis, synovitis, arthropathy, anti-cyclic citrullinated peptide antibodies, and anti-citrullinated protein antibody - ACPA...
September 30, 2018: Scandinavian Journal of Rheumatology
https://www.readbyqxmd.com/read/30267550/the-immunome-of-soybean-allergy-comprehensive-identification-and-characterization-of-epitopes
#19
Karolin Kern, Heide Havenith, Nicolas Delaroque, Paul Rautenberger, Jörg Lehmann, Markus Fischer, Holger Spiegel, Stefan Schillberg, Eva Ehrentreich-Foerster, Stefanie Aurich, Regina Treudler, Michael Szardenings
BACKGROUND: The precise mapping of multiple antibody epitopes recognized by patients' sera allows a more detailed and differentiated understanding of immunologic diseases. It may lead to the development of novel therapies and diagnostic tools. OBJECTIVE: Mapping soybean specific epitopes relevant for soybean allergy patients and persons sensitized to soybean, and analysis of their IgE/IgG binding spectrum. METHODS: Identification of epitopes using sera, applying an optimized peptide-phage display library followed by next-generation sequencing, specially designed in silico data analysis and subsequent peptide microarray analysis...
September 29, 2018: Clinical and Experimental Allergy: Journal of the British Society for Allergy and Clinical Immunology
https://www.readbyqxmd.com/read/30262856/identification-of-peptide-mimotope-ligands-for-natalizumab
#20
Laura E Ruff, Jessica A Pfeilsticker, Nicholas E Johnsen, Sarah Nocchi, Bradley T Messmer
Mimotope peptides selected from combinatorial peptide libraries can be used as capture reagents for immunoassay detection of therapeutic monoclonal antibodies (mAbs). We report the use of phage display libraries to identify peptide ligands (VeritopesTM ) that bind natalizumab, a therapeutic mAb indicated for use in multiple sclerosis. PKNPSKF is identified as a novel natalizumab-binding motif, and peptides containing this motif demonstrated utility as capture reagents in enzyme-linked immunosorbent assays (ELISAs)...
September 27, 2018: Scientific Reports
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