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Milk Protein

Fatemeh Sharifpanah, Enas Hussein Ali, Maria Wartenberg, Heinrich Sauer
The milk thistle compound Silibinin (i.e., a 1:1 mixture of Silybin A and Silybin B) stimulates vasculogenesis of mouse embryonic stem (ES) cells. Because vasculogenesis and leukopoiesis are interrelated, the effect of Silibinin on leukopoiesis of ES cells was investigated. Treatment of differentiating ES cells with hydrosoluble Silibinin-C-2',3-dihydrogen succinate dose-dependent increased the number of CD18+ , CD45+ , and CD68+ cells, indicating leukocyte/macrophage differentiation. Silibinin treatment activated phosphoinositide 3-kinase (PI3K), AKT (protein kinase B), signal transducer and activator of transcription 3 (STAT3), stimulated hypoxia-induced factor-1α (HIF-1α), and vascular endothelial growth factor receptor 2 (VEGFR2) expression and raised intracellular nitric oxide (NO)...
December 11, 2018: Phytotherapy Research: PTR
Chanbora Chea, Sivmeng Haing, Mutsumi Miyauchi, Madhu Shrestha, Hiromichi Imanaka, Takashi Takata
Osteosarcoma (OS) is one the most common primary malignancies of the bone in children and young adults with high metastasis. The use of non-toxic naturally derived compounds is one of present strategies in OS therapy to reduce secondary effects and chemo-resistance. Lactoferrin (LF), a transferrin protein derived from milk, currently appears to be an anticancer agent. However, its suppressive effects on OS have not been fully investigated. Therefore, we aimed to examine the molecular mechanism underlying the inhibitory effects of bovine LF (bLF) on OS...
December 10, 2018: Biochemical and Biophysical Research Communications
Lukáš Kučera, Jaroslav Peška, Pavel Fojtík, Petr Barták, Diana Sokolovská, Jaroslav Pavelka, Veronika Komárková, Jaromír Beneš, Lenka Polcerová, Miroslav Králík, Petr Bednář
In this study, a soil from two ceramic vessels belonging to Corded Ware culture, 2707⁻2571 B.C., found in a cremation grave discovered in Central Moravia, Czech Republic, was analyzed using matrix-assisted laser desorption/ionization⁻mass spectrometry (MALDI⁻MS) combined with advanced statistical treatment (principal component analysis, PCA, and orthogonal projection to latent structures discriminant analysis, OPLS-DA) and by enzyme-linked immunosorbent assay (ELISA). MALDI⁻MS revealed the presence of triacylglycerols in both vessels...
December 7, 2018: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
Song-Hwa Bae, Jin Yu, Tae Geol Lee, Soo-Jin Choi
Because of their nutritional value, zinc oxide (ZnO) nanoparticles (NPs) are applied as a dietary source of zinc, by direct addition to complex, multiple-component food matrices. The thereby occurring interactions of NPs with food matrices may have biological or toxic effects. In particular, NP interactions with food protein can lead to structural deformation of the latter, potentially changing its digestive efficiency and gastrointestinal absorption. In this study, interactions between ZnO NPs and a representative complex protein food matrix, skim milk, were compared with those between NPs and individual components of this food matrix (i...
December 7, 2018: International Journal of Molecular Sciences
Xin-Jun Du, Xiao-Yi Wang, Xuan Dong, Ping Li, Shuo Wang
Strong desiccation tolerance is an outstanding feature of Cronobacter sakazakii and can enable the bacterium to survive in a dry food matrix (such as milk powder) for a long time. Therefore, contamination of food possessing low water activity with C. sakazakii can increase the risk of infection in human beings, particularly in neonates and infants. However, the mechanism underlying the desiccation tolerance property of C. sakazakii is largely unknown. In this study, the desiccation tolerance characteristics of 42 C...
2018: Frontiers in Microbiology
Goutam Banerjee, Atish Ray, Kazi Nurul Hasan
β-Galactosidase is a metal-activated enzyme, which breaks down the glucosidic bond of lactose and produces glucose and galactose. Among several commercial applications, preparation of lactose-free milk has gained special attention. The present objective is to demonstrate the activity kinetics of β-galactosidase purified from a non-pathogenic bacterium Arthrobacter oxydans SB. The enzyme was purified by DEAE-cellulose and Sephadex G-100 column chromatography. The purity of the protein was checked by high-performance liquid chromatography (HPLC)...
December 2018: Journal of Biosciences
Daniel Klotz, Marie Schreiner, Valeria Falcone, Daniel Jonas, Mirjam Kunze, Andrea Weber, Hans Fuchs, Roland Hentschel
Background: Human milk (HM) for preterm infants will often be pasteurized for cytomegalovirus (CMV) inactivation and reduction of its bacterial count. High-temperature short-time (HTST) treatment compared to standard Holder pasteurization (HoP) reduces the impact of heat treatment on bioactive HM proteins while effectively inactivating CMV. No data are available for the efficacy of bacterial count reduction using HTST treatments that are available for clinical use. Objective: To test the antiviral and antibacterial efficacy of HTST treatment protocols in HM using a modified HTST treatment device compared to standard HoP...
2018: Frontiers in Pediatrics
Jana Buresova, Petra Janovska, Ondrej Kuda, Jana Krizova, Inge Romijnders-van der Stelt, Jaap Keijer, Hana Hansikova, Martin Rossmeisl, Jan Kopecky
BACKGROUND/OBJECTIVE: Adaptation to the extrauterine environment depends on a switch from glycolysis to catabolism of fatty acids (FA) provided as milk lipids. We sought to learn whether the postnatal induction of muscle FA oxidation in mice could reflect propensity to obesity and to characterize the mechanisms controlling this induction. METHODS: Experiments were conducted using obesity-resistant A/J and obesity-prone C57BL/6J (B6) mice maintained at 30 °C, from 5 to 28 days after birth...
December 11, 2018: International Journal of Obesity: Journal of the International Association for the Study of Obesity
M Vailati-Riboni, R E Bucktrout, S Zhan, A Geiger, J C McCann, R M Akers, J J Loor
BACKGROUND: To reduce costs of rearing replacement heifers, researchers have focused on decreasing age at breeding and first calving. To increase returns upon initiation of lactation the focus has been on increasing mammary development prior to onset of first lactation. Enhanced plane of nutrition pre-weaning may benefit the entire replacement heifer operation by promoting mammary gland development and greater future production. METHODS: Twelve Holstein heifer calves (< 1 week old) were reared on 1 of 2 dietary treatments (n = 6/group) for 8 weeks: a control group fed a restricted milk replacer at 0...
December 11, 2018: BMC Genomics
Hao Zhang, Yue Li, Yueping Chen, Lili Zhang, Tian Wang
PURPOSE: Intrauterine growth retardation (IUGR) is detrimental to the intestinal development of neonates, yet satisfactory treatment strategies remain limited. This study was, therefore, conducted using neonatal piglets as a model to investigate the potential of N-acetylcysteine (NAC) to alleviate intestinal damage caused by IUGR. METHODS: Seven normal birth weight (NBW) and fourteen IUGR neonatal male piglets were selected and then fed a basal milk diet (NBW-CON and IUGR-CON groups) or a basal milk diet supplemented with 1...
December 10, 2018: European Journal of Nutrition
T F Pedersen, C Y Chang, N L Trottier, T S Bruun, P K Theil
The objective of the current study was to quantify loss of energy in feces, urine, heat and milk, to evaluate feed efficiency and to evaluate optimal ratio of dietary CP to energy for lactating sows fed increasing dietary CP. A total of 72 sows were included in the experiment from d 2 after parturition until weaning at d 28. Sows were allocated to six dietary treatments formulated to be isocaloric (9.8 MJ NE/kg) and increasing SID CP (11.8, 12.8, 13.4, 14.0, 14.7, and 15.6% SID CP). Sows were weighed and back fat scanned within 2 d after farrowing, at d 18 ± 3 and d 28 ± 3...
December 7, 2018: Journal of Animal Science
Reinette Tydeman-Edwards, Francois Cornel Van Rooyen, Corinna May Walsh
This study compared the diet and anthropometric status of adults (25-64 years) in rural and urban South Africa. Anthropometric status of adults and preschool children (<7 years old) from the same households were also determined. A descriptive cross-sectional design was applied. All adults from three towns in the rural southern Free State (n = 553) and a stratified proportional cluster sample from urban Mangaung (n = 419) participated. Anthropometric assessments included body mass index and waist circumference...
December 2018: Heliyon
Sandra Rayen Quilodran-Vega, Leonardo Albarracin, Elvira Maria Hebert, Lucila Saavedra, Alexis Fonseca, Alexis Salas-Burgos, Haruki Kitazawa, Julio Villena
This report describes the draft genome sequence of Lactobacillus brevis TUCO-5E, a probiotic strain isolated from porcine maternal milk. The reads were generated by a whole-genome sequencing (WGS) strategy on an Illumina MiSeq sequencer and were assembled into contigs with a total estimated size of 2,461,089 bp. A total of 2,455 open reading frames (ORFs) were predicted, including 2,301 protein-coding sequences. The draft genome sequence of L. brevis TUCO-5E will be useful for further studies of specific genetic features and for understanding the mechanisms of its probiotic properties in the porcine host...
November 2018: Microbiology resource announcements
T Sinai, M R Goldberg, L Nachshon, R Amitzur-Levy, T Yichie, Y Katz, E Monsonego-Ornan, A Elizur
BACKGROUND: Growth impairment was previously described in milk-allergic children but was not examined in adults upon reaching final height. OBJECTIVES: To investigate the dietary intake and final stature of young adults with IgE-mediated cow's milk-allergy (IgE-CMA) as compared to non-allergic controls. METHODS: 87 IgE-CMA patients, median age 19.5 years (IQR, 17.3-22.7) and 36 control participants without food allergies, median age 22.7 years (IQR, 18...
December 6, 2018: Journal of Allergy and Clinical Immunology in Practice
Mayte Suárez-Fariñas, Maria Suprun, Helena L Chang, Gustavo Gimenez, Galina Grishina, Robert Getts, Kari Nadeau, Robert A Wood, Hugh A Sampson
BACKGROUND: In a recent trial of milk oral immunotherapy (MOIT) with or without omalizumab in 55 patients with milk allergy treated for 28 months, 44 of 55 subjects passed a 10-g desensitization milk protein challenge; 23 of 55 subjects passed the 10-g sustained unresponsiveness (SU) challenge 8 weeks after discontinuing MOIT. OBJECTIVE: We sought to determine whether IgE and IgG4 antibody binding to allergenic milk protein epitopes changes with MOIT and whether this could predict the development of SU...
December 7, 2018: Journal of Allergy and Clinical Immunology
B N Shonka-Martin, A R Hazel, B J Heins, L B Hansen
Rotational crossbred cows of the Montbéliarde, Viking Red, and Holstein (HO) breeds (CB) were compared with HO cows for dry matter intake (DMI), body weight (BW), cow height, body condition score (BCS), and production during the first 150 d of first, second, and third lactations. Primiparous and multiparous CB (n = 63 and 43, respectively) and HO (n = 60 and 37, respectively) cows calved from September 2014 to June 2017. Cows were fed the same total mixed ration twice daily, with refusals weighed once daily...
October 24, 2018: Journal of Dairy Science
K Nichols, J Dijkstra, H van Laar, S Pacheco, H J van Valenberg, A Bannink
This study tested the effects of energy from glucogenic (glucose; GG) or lipogenic (palm olein; LG) substrates at low (LMP) and high (HMP) metabolizable protein levels on whole-body energy and N partitioning of dairy cattle. Six rumen-fistulated, second-lactation Holstein-Friesian dairy cows (97 ± 13 d in milk) were randomly assigned to a 6 × 6 Latin square design in which each experimental period consisted of 5 d of continuous abomasal infusion followed by 2 d of rest. A total mixed ration consisting of 42% corn silage, 31% grass silage, and 27% concentrate (dry matter basis) was formulated to meet 100 and 83% of net energy and metabolizable protein requirements, respectively, and was fed at 90% of ad libitum intake by individual cow...
October 24, 2018: Journal of Dairy Science
J de Souza, A L Lock
The objective of our study was to evaluate the effects of timing of palmitic acid (C16:0) supplementation on production responses of early-lactation dairy cows. Fifty-two multiparous cows were used in a randomized complete block design experiment. During the fresh period (FR; 1-24 d in milk) cows were assigned to either a control diet containing no supplemental fat (CON) or a diet supplemented with C16:0 (palmitic acid, PA; 1.5% of diet dry matter). During the peak (PK) period (25-67 d in milk) cows were assigned to either a CON diet or a PA (1...
October 24, 2018: Journal of Dairy Science
Agnieszka Bzikowska, Aneta Czerwonogrodzka-Senczyna, Halina Weker, Aleksandra Wesołowska
Background: Human milk is the optimal nutrition for newborns and infants during the first period of their life – from birth to 6-th month. It contains a uniquely quantitative and qualitative balanced nutrients profile. Composition of breast milk is dynamic and may vary according to maternal nutritional status. Objective: The aim of this study was to investigate associations between human milk composition and maternal nutritional status. Material and methods: One-day milk samples were provided by exclusively breastfeeding mothers (n=40) at the first month of lactation...
2018: Roczniki Państwowego Zakładu Higieny
Hak-Jae Chung, Hyun-Jung Park, Sun-Young Baek, Jin-Ki Park, Won-Young Lee, Kyung-Woon Kim, Yu-Mi Jo, Shinichi Hochi, Yong-Min Kim, Tae-Jeong Choi, Eun-Suek Cho, Kyu-Ho Cho
Tissue plasminogen activator (tPA) is a protein involved in the breakdown of blood clots. We have previously produced a human tPA (htPA)-overexpressing transgenic pig using a mammary gland-specific promoter. In this study, we have established a transgenic pig mammary gland cell line that produces recombinant htPA. The mammary gland cells grew well and retained their character over long periods of culture. There was no difference in the extent of apoptosis in transgenic cells compared to wild-type mammary gland cells...
December 6, 2018: Animal Biotechnology
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