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Microbiology space

Vincenzo Zammuto, Felix M Fuchs, Marcel Fiebrandt, Katharina Stapelmann, Nikea J Ulrich, Teresa L Maugeri, Rüdiger Pukall, Concetta Gugliandolo, Ralf Moeller
Submarine hydrothermal vents are inhabited by a variety of microorganisms capable of tolerating environmental extremes making them ideal candidates to further expand our knowledge of the limitations for terrestrial life, including their ability to survive the exposure of spaceflight-relevant conditions. The spore resistance of two Bacillus spp. strains, APA and SBP3, isolated from two shallow vents off Panarea Island (Aeolian Islands, Italy), to artificial and environmental stressors (i.e., UVC radiation, X-rays, heat, space vacuum, hydrogen peroxide [H2 O2 ], and low-pressure plasma), was compared with that of two close phylogenetic relatives (Bacillus horneckiae and Bacillus oceanisediminis)...
October 5, 2018: Astrobiology
Ayodele Sasegbon, Laura O'Shea, Shaheen Hamdy
Introduction: Elderly people are recognised to be at increased risk of oropharyngeal dysphagia (OPD), the causes of which are multifactorial. Our aim was to identify if sepsis is associated with OPD in the elderly during hospitalisation in the absence of known other risk factors for OPD. Methods: A hospital electronic database was searched for elderly patients (≥65 years) referred for assessment for suspected dysphagia between March 2013 and 2014. Exclusion criteria were age <65 years, pre-existing OPD or acute OPD secondary to acute intracranial event, space-occupying lesion or trauma...
October 2018: Frontline Gastroenterology
F Riechelmann, P Kaiser, R Arora
OBJECTIVE: Debridement of soft tissue and bone in an open fracture situation to minimize infection risk and achieve primary skin closure, or to provide conditions for early soft tissue coverage. INDICATIONS: Indications are Gustilo-Anderson grade I-III A-C open fractures. CONTRAINDICATIONS: Contraindications are injuries requiring amputation, burns, and life-threatening injuries which make appropriate treatment temporarily impossible. SURGICAL TECHNIQUE: Removal of gross contamination and macroscopic contaminants; debridement of the wound; complete resection of contaminated and dirty tissue; sparse step-by-step resection of contaminated or non-vital wound and bone margins until vital, bleeding tissue begins; low-pressure irrigation with isotonic irrigation fluid; diagnostic biopsies for microbiological testing; reduction of dead space by interpositioning of muscle or cement spacers loaded with local antibiotics; primary wound closure if tension-free closure possible; otherwise, if resources and knowhow permit and satisfactory clean debridement was achieved, local flap; if flap impossible, debridement not satisfactory, secondary tissue necrosis likely, potential remaining contamination or contamination with fecal matter, then vacuum-assisted closure therapy...
October 2018: Operative Orthopädie und Traumatologie
Anette McLeod, Kristian Hovde Liland, John-Erik Haugen, Oddvin Sørheim, Kristine S Myhrer, Askild L Holck
We have compared the efficacy of continuous ultraviolet (UV-C) (254 nm) and pulsed UV light in reducing the viability of Salmonella Enteritidis, Listeria monocytogenes , Staphylococcus aureus , enterohemorrhagic Escherichia coli , Pseudomonas spp., Brochothrix thermospacta, Carnobacterium divergens , and extended-spectrum β-lactamase producing E. coli inoculated on chicken fillet surface. Fluences from 0.05 to 3.0 J/cm2 (10 mW/cm2 , from 5 to 300 s) used for UV-C light resulted in average reductions from 1...
February 2018: Journal of Food Safety
Adrian Caceres, Maria Luisa Avila, Marco Luis Herrera
INTRODUCTION: Invasive mycosis of the central nervous system represent a diverse group of diseases that have gradually emerged as not only opportunistic infections in patients with immune susceptibility due to congenital and acquired deficiency, immunomodulation, solid organ and stem cell transplantation, hematological malignancies, and chronic steroid use but also in selected risk populations such as low weight preterm infants, patients with shunted hydrocephalus and external ventricular drainages, skull base surgery, and head injury...
October 2018: Child's Nervous System: ChNS: Official Journal of the International Society for Pediatric Neurosurgery
Neelam Shakya, Divashree Sharma, Vilas Newaskar, Deepak Agrawal, Sanket Shrivastava, Rashi Yadav
Introduction: Odontogenic infections are the most commonly encountered orofacial infections, which may spread into the adjacent anatomical spaces along the contiguous fascial planes, leading to involvement of multiple spaces which can progress to life-threatening situations. Materials and Methods: A prospective study was carried out on 100 consecutive cases of odontogenic infections treated at our institute over a period of 18 months by surgical intervention and intravenous antibiotics...
September 2018: Journal of Maxillofacial and Oral Surgery
Marlina E Lovett, Zubin S Shah, Melissa Moore-Clingenpeel, Eric Sribnick, Adam Ostendorf, Melissa G Chung, Jeffrey Leonard, Nicole F O'Brien
OBJECTIVE Focal intracranial infections such as intraparenchymal abscesses or localized infections in the epidural or subdural spaces are relatively rare infections associated with both morbidity and mortality in children. Although children with these infections frequently require surgical intervention, there is a paucity of literature describing the critical care resources required to manage these cases. This retrospective chart review was performed to evaluate the resources necessary to care for critically ill children with focal intracranial infections at the authors' institution...
July 13, 2018: Journal of Neurosurgery. Pediatrics
Kai-Alexander Saalbach, Jens Twiefel, Jörg Wallaschek
The generation of cavitation by ultrasound is used in a large number of processes in different scientific and industrial applications. Chemical reactions are made possible or accelerated by locally occurring high temperatures and pressures generated by the collapse of cavitation bubbles. Mixing or separating substances, emulsification, ultrasonic cleaning, degassing, and microbiological treatment of fluids are some more applications for ultrasonic generated cavitation. In most of these applications, an optical examination of the events within the cavitating medium is not possible...
June 28, 2018: Ultrasonics
D Vu, M Groenewald, M de Vries, T Gehrmann, B Stielow, U Eberhardt, A Al-Hatmi, J Z Groenewald, G Cardinali, J Houbraken, T Boekhout, P W Crous, V Robert, G J M Verkley
Species identification lies at the heart of biodiversity studies that has in recent years favoured DNA-based approaches. Microbial Biological Resource Centres are a rich source for diverse and high-quality reference materials in microbiology, and yet the strains preserved in these biobanks have been exploited only on a limited scale to generate DNA barcodes. As part of a project funded in the Netherlands to barcode specimens of major national biobanks, sequences of two nuclear ribosomal genetic markers, the Internal Transcribed Spaces and 5...
March 2019: Studies in Mycology
Agata Bielawska-Drózd, Piotr Cieślik, Justyna Bohacz, Teresa Korniłłowicz-Kowalska, Dorota Żakowska, Michał Bartoszcze, Bożena Wlizło-Skowronek, Izabela Winnicka, Marek Brytan, Leszek Kubiak, Ewa Skopińska-Różewska, Janusz Kocik
INTRODUCTION AND OBJECTIVE: The goal of the study was a microbiological, qualitative and quantitative analysis of bioaerosol at the workplace of medical personnel (Health Emergency Departments (HEDs), ambulances), and comparative administration offices with an expected neutral occupational exposure to biological agents measured with individual Button Sampler. MATERIAL AND METHODS: Personal sampling was performed with Button Sampler instrument loaded with gelatine filters in 10 HEDs, in 9 ambulances and in 9 offices to assess the occupational biological agents' exposure in air...
June 20, 2018: Annals of Agricultural and Environmental Medicine: AAEM
Caroline Charlier, Sylvain Poirée, Christophe Delavaud, Gaby Khoury, Clémence Richaud, Alexandre Leclercq, Olivier Hélénon, Marc Lecuit
Background: Neurolisteriosis ranks among the most severe neurological infections. Its radiological features have not been thoroughly studied. We describe here the neuroradiological features of neurolisteriosis and assess their prognostic value. Methods: Patients with microbiologically proven neurolisteriosis were enrolled from November 2009 to October 2013 in MONALISA study. Magnetic resonance and computed tomography images were studied by 2 independent neuroradiologists...
October 15, 2018: Clinical Infectious Diseases: An Official Publication of the Infectious Diseases Society of America
Cristina Serraino, Chiara Elia, Christian Bracco, Gianluca Rinaldi, Fulvio Pomero, Alberto Silvestri, Remo Melchio, Luigi Maria Fenoglio
Pyogenic liver abscess (PLA) are space-occupying lesions in the liver associated with high morbidity and mortality. The aim of this study is to review an Italian hospital experience in epidemiological, clinical patterns, and management of PLA.We performed a retrospective, descriptive case series at a single center assessing demographic characteristics, presentation patterns, etiological factors, microbiological etiology, and management for patients treated for PLA between 2000 and 2016.Around 109 patients were identified...
May 2018: Medicine (Baltimore)
Tilmann Kuenzl, Xiaochun Li-Blatter, Puneet Srivastava, Piet Herdewijn, Timothy Sharpe, Sven Panke
The import of nonnatural molecules is a recurring problem in fundamental and applied aspects of microbiology. The dipeptide permease (Dpp) of Escherichia coli is an ABC-type multicomponent transporter system located in the cytoplasmic membrane, which is capable of transporting a wide range of di- and tripeptides with structurally and chemically diverse amino acid side chains into the cell. Given this low degree of specificity, Dpp was previously used as an entry gate to deliver natural and nonnatural cargo molecules into the cell by attaching them to amino acid side chains of peptides, in particular, the γ-carboxyl group of glutamate residues...
July 1, 2018: Applied and Environmental Microbiology
Igor Kljujev, Vera Raicevic, Jelena Jovicic-Petrovic, Bojana Vujovic, Milica Mirkovic, Michael Rothballer
The microbiologically contaminated vegetables represent a risk for consumers, especially vegetables without thermal processing. It is known that human pathogen bacteria, such as Listeria monocytogenes, could exist on fresh vegetables. The fresh vegetables could become Listeria-contaminated if they come in touch with contaminated soil, manure, irrigation water. The aim of this work was to investigate the presence of Listeria spp. and L. monocytogenes in different kind of vegetables grown in field and greenhouse condition as well as surface and endophytic colonization plant roots of different vegetables species by L...
April 22, 2018: Microbial Pathogenesis
Haoyu Sun, Edward J Calabrese, Min Zheng, Dali Wang, Yongzheng Pan, Zhifen Lin, Ying Liu
Hormesis occurs frequently in broadly ranging biological areas (e.g. plant biology, microbiology, biogerontology), toxicology, pharmacology and medicine. While numerous mechanisms (e.g. receptor and pathway mediated pathway responses) account for stimulatory and inhibitory features of hormetic dose responses, the vast majority emphasizes the inclusion of many doses but only one timepoint or use of a single optimized dose that is assessed over a broad range of timepoints. In this paper, a toxicity study was designed using a large number of properly spaced doses with responses determined over a large number of timepoints, which could help us reveal the underlying mechanism of hormesis...
August 2018: Chemosphere
Margarita Trobos, Martin Lars Johansson, Sofia Jonhede, Hanna Peters, Maria Hoffman, Omar Omar, Peter Thomsen, Malou Hultcrantz
PURPOSE: In this prospective clinical pilot study, abutments with different topologies (machined versus polished) were compared with respect to the clinical outcome and the microbiological profile. Furthermore, three different sampling methods (retrieval of abutment, collection of peri-abutment exudate using paper-points, and a small peri-abutment soft-tissue biopsy) were evaluated for the identification and quantification of colonising bacteria. METHODS: Twelve patients, seven with machined abutment and five with polished abutment, were included in the analysis...
June 2018: European Archives of Oto-rhino-laryngology
Irem Serifoglu, Balam Er Dedekarginoglu, Ebru Hatice Ayvazoglu Soy, Gaye Ulubay, Mehmet Haberal
OBJECTIVES: Hemoptysis is a symptom that can be caused by airway disease, pulmonary parenchymal disease, or pulmonary vascular disease, or it can be idiopathic. Infection is the most common cause of hemoptysis, accounting for 60% to 70% of cases. Hemoptysis is also an initial symptom of diffuse alveolar hemorrhage syndrome, although it may be absent at presentation in one-third of patients. Diffuse alveolar hemorrhage is characterized by disruption of the alveolar-capillary basement membranes because of either injury or inflammation of the arterioles, venules, or capillaries, resulting in bleeding in alveolar spaces...
March 2018: Experimental and Clinical Transplantation
Tianlong Zheng, Pengyu Li, Wenjun Wu, Jianguo Liu, Zhining Shi, Xuesong Guo, Junxin Liu
With rapid industrialization and urbanization in the nineteenth century, the activated sludge process (ASP) has experienced significant steps forward in the face of greater awareness of and sensitivity toward water-related environmental problems. Compared with conventional flocculent ASP, the major advantages of granular sludge are characterized by space saving and resource recovery, where the methane and hydrogen recovery in anaerobic granular and 50% more space saving, 30-50% of energy consumption reduction, 75% of footprint cutting, and even alginate recovery in aerobic granular...
April 2018: Applied Microbiology and Biotechnology
Dae Woon Song, Byung Kwan Park, Suk Won Suh, Seung Eun Lee, Jong Won Kim, Joong-Min Park, Hye Ryoun Kim, Mi-Kyung Lee, Yoo Shin Choi, Beom Gyu Kim, Yong Gum Park
PURPOSE: Essential treatment of acute appendicitis is surgical resection with the use of appropriate antibiotics. In order to effectively treat acute appendicitis, it is important to identify the microorganism of acute appendicitis and evaluate the effective antibiotics. METHODS: A total of 694 patients who underwent appendectomy for acute appendicitis and had positive microbial result between 2006 and 2015 were recruited. For microbial assessment, luminal contents of the appendix were swabbed after appendectomy...
April 2018: International Journal of Colorectal Disease
Jared L Wilmoth, Peter W Doak, Andrea Timm, Michelle Halsted, John D Anderson, Marta Ginovart, Clara Prats, Xavier Portell, Scott T Retterer, Miguel Fuentes-Cabrera
The factors leading to changes in the organization of microbial assemblages at fine spatial scales are not well characterized or understood. However, they are expected to guide the succession of community development and function toward specific outcomes that could impact human health and the environment. In this study, we put forward a combined experimental and agent-based modeling framework and use it to interpret unique spatial organization patterns of H1-Type VI secretion system (T6SS) mutants of P . aeruginosa under spatial confinement...
2018: Frontiers in Microbiology
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