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https://www.readbyqxmd.com/read/29792924/creation-of-an-acellular-vaginal-matrix-for-potential-vaginal-augmentation-and-cloacal-repair
#1
K V Greco, L G Jones, I Obiri-Yeboa, T Ansari
STUDY OBJECTIVE: our aim was to use porcine vagina to create a vaginal matrix and test its cellular biocompatibility. DESIGN: vagina was harvested from pigs and de-cellularised (DC) using a combination of detergents (Triton x-100 and sodium deoxycholate) and enzymes (DNAse/RNAse). INTERVENTIONS: the presence of cellular material, collagen structural integrity and basement membrane proteins were assessed histologically. To address cytocompatibility, porcine adipose derived-mesenchymal stem cells (AD-MSC) were harvested from abdominal fat together with vaginal epithelial cells (VEC) and seeded onto the mucosal aspect of the vaginal scaffold...
May 21, 2018: Journal of Pediatric and Adolescent Gynecology
https://www.readbyqxmd.com/read/29791775/vaginal-microbiome-in-early-pregnancy-and-subsequent-risk-of-spontaneous-preterm-birth-a-case-control-study
#2
Negar Tabatabaei, A Murat Eren, Luis B Barreiro, Vania Yotova, Anne Dumaine, Catherine Allard, William D Fraser
OBJECTIVES: To explore differences in vaginal microbiome between preterm and term deliveries. DESIGN: Nested case-control study in 3D cohort. SETTING: Quebec, Canada. SAMPLE: Ninety-four cases with spontaneous preterm birth (17 early (<34 wk) and 77 late (34-36 wk) preterm birth) and 356 controls with term delivery (≥37 wk). METHODS: To assess vaginal microbiome by sequencing the V4 region of 16S ribosomal-RNA (rRNA) gene in swabs self-collected early pregnancy...
May 23, 2018: BJOG: An International Journal of Obstetrics and Gynaecology
https://www.readbyqxmd.com/read/29790451/genital-mycoplasmas-of-healthy-bitches
#3
Zinka Maksimović, Alan Maksimović, Anis Halilbašić, Maid Rifatbegović
Little is known about the presence of mycoplasmas in the genital tracts of domestic and stray bitches or in the vaginas of ovariohysterectomized (OHE) bitches. Moreover, to our knowledge, there has been no research to investigate the presence of canine vaginal mycoplasmas during the different stages of the reproductive cycle. We investigated the occurrence of mycoplasmas in the vaginas of healthy domestic and stray intact bitches, to correlate their presence with specific stages of the reproductive cycle, and to compare them with those in OHE bitches...
May 1, 2018: Journal of Veterinary Diagnostic Investigation
https://www.readbyqxmd.com/read/29787390/genitourinary-syndrome-of-menopause
#4
Jan L Shifren
Genitourinary syndrome of menopause (GSM) describes a collection of exam findings and bothersome symptoms associated with estrogen deficiency involving changes to the labia, introitus, clitoris, vagina, urethra, and bladder. Vulvovaginal atrophy is a component of GSM. GSM is a highly prevalent medical condition with adverse effects on the health and quality of life of midlife women. There are many effective treatment options, including nonhormonal lubricants and moisturizers, physical therapy, low-dose vaginal estrogen therapy, vaginal dehydroepiandrosterone, and oral ospemifene...
May 21, 2018: Clinical Obstetrics and Gynecology
https://www.readbyqxmd.com/read/29785383/valentine-s-day-misadventure-missing-vagina-for-urethra
#5
Ahmed Kamil, Claudiu Cozman, Asadullah Aslam, Nadeem Nusrat, Syed Jaffry
No abstract text is available yet for this article.
May 2018: Urology Case Reports
https://www.readbyqxmd.com/read/29782463/gut-microbiota-probiotics-and-sport-from-clinical-evidence-to-agonistic-performance
#6
Marco Pane, Angela Amoruso, Francesca Deidda, Teresa Graziano, Serena Allesina, Luca Mogna
Human beings harbor clusters of bacteria in different parts of the body, such as the surface or the deep layers of the skin, the mouth, the lungs, the intestine, the vagina, and all the surfaces exposed to the outer world. The majority of microbes resides in the gut, have a weighty influence on human physiology and nutrition and are vital for human life. There is growing evidence showing that the gut microbiota plays important roles in the maturation of the immune system and the protection against some infectious agents...
May 18, 2018: Journal of Clinical Gastroenterology
https://www.readbyqxmd.com/read/29781567/localized-vaginal-uterine-rhabdomyosarcoma-results-of-a-pooled-analysis-from-four-international-cooperative-groups
#7
Veronique Minard-Colin, David Walterhouse, Gianni Bisogno, Helene Martelli, James Anderson, David A Rodeberg, Andrea Ferrari, Meriel Jenney, Suzanne Wolden, Gianluca De Salvo, Carola Arndt, Johannes H M Merks, Soledad Gallego, Dominique Schwob, Christine Haie-Meder, Christophe Bergeron, Michael C G Stevens, Odile Oberlin, Douglas Hawkins
BACKGROUND: Vaginal/uterine rhabdomyosarcoma (VU RMS) is one of the most favorable RMS sites. To determine the optimal therapy, the experience of four cooperative groups (Children's Oncology Group [COG], International Society of Pediatric Oncology (SIOP) Malignant Mesenchymal Tumor Group [MMT], Italian Cooperative Soft Tissue Sarcoma Group [ICG], and European pediatric Soft tissue sarcoma Study Group [EpSSG]) was analyzed. PROCEDURE: From 1981 to 2009, 237 patients were identified...
May 21, 2018: Pediatric Blood & Cancer
https://www.readbyqxmd.com/read/29780785/an-extremely-rare-case-of-hand-prolapse-with-preterm-premature-rupture-in-the-membrane-of-one-twin
#8
Minji Kim, Hye-Joo Lee, Suk-Joo Choi, Soo-Young Oh, Cheong-Rae Roh, Jong-Hwa Kim
The latency in preterm premature rupture of membranes (PPROM) can last for weeks. We describe an extremely rare case of hand prolapse with PPROM that was exposed for 23 days before delivery. The patient had spontaneous PPROM of twin A at 21.4 weeks of gestation with shoulder presentation. The right arm of the fetus eventually protruded out the vagina and the hand was exposed for extended period of time of 23 days until delivery. Daily dressing by applying collagen to dry skin and silicone to keep moisture was done to the protruding hand to prevent dehydration and desquamation of the skin...
May 2018: Obstetrics & Gynecology Science
https://www.readbyqxmd.com/read/29779513/common-sexually-transmitted-infections-in-women
#9
REVIEW
Ashley L Hodges, Aimee Chism Holland
The spread of sexually transmitted infections (STIs) remains a significant public health issue in the United States. Social, economic, and behavioral implications affecting the spread of STIs have been identified. The most important social factor in the United States is the stigma associated with discussing sex and STI screening. In this article, specific recommendations for women are included regarding screening, diagnosing, and treating common vaginal and cervical infections. Screening women for infections of the vagina and cervix is essential because untreated infections may result in complications that have current and long-term health consequences and impact quality of life...
June 2018: Nursing Clinics of North America
https://www.readbyqxmd.com/read/29771989/bacterial-dna-is-present-in-the-fetal-intestine-and-overlaps-with-that-in-the-placenta-in-mice
#10
Keith A Martinez, Joann Romano-Keeler, Joseph P Zackular, Daniel J Moore, Robert M Brucker, Christopher Hooper, Shufang Meng, Naoko Brown, Simon Mallal, Jeff Reese, David M Aronoff, Hakdong Shin, Maria Gloria Dominguez-Bello, Jörn-Hendrik Weitkamp
Bacterial DNA has been reported in the placenta and amniotic fluid by several independent groups of investigators. However, it's taxonomic overlap with fetal and maternal bacterial DNA in different sites has been poorly characterized. Here, we determined the presence of bacterial DNA in the intestines and placentas of fetal mice at gestational day 17 (n = 13). These were compared to newborn intestines (n = 15), maternal sites (mouth, n = 6; vagina, n = 6; colon, n = 7; feces, n = 8), and negative controls to rule out contamination...
2018: PloS One
https://www.readbyqxmd.com/read/29770881/robot-assisted-laparoscopic-resection-of-clinical-t4b-tumours-of-distal-sigmoid-and-rectum-initial-results
#11
Rogier M P H Crolla, Janneke J C Tersteeg, George P van der Schelling, Jan H Wijsman, Jennifer M J Schreinemakers
BACKGROUND: Radical resection by multivisceral resection of colorectal T4 tumours is important to reduce local recurrence and improve survival. Oncological safety of laparoscopic resection of T4 tumours is controversial. However, robot-assisted resections might have advantages, such as 3D view and greater range of motion of instruments. The aim of this study is to evaluate the initial results of robot-assisted resection of T4 rectal and distal sigmoid tumours. METHODS: This is a cohort study of a prospectively kept database of all robot-assisted rectal and sigmoid resections between 2012 and 2017...
May 16, 2018: Surgical Endoscopy
https://www.readbyqxmd.com/read/29770629/predicting-factors-for-resumption-of-spontaneous-voiding-following-nerve-sparing-radical-hysterectomy
#12
Chalaithorn Nantasupha, Kittipat Charoenkwan
OBJECTIVE: To determine factors affecting voiding recovery on the day of Foley catheter removal (postoperative day 7, POD7) after nerve-sparing radical hysterectomy (NSRH) for early-stage cervical cancer. METHODS: Early-stage cervical cancer patients, who underwent type C1 radical hysterectomy between January 2006 and June 2016 were included. Clinical and pathological data were reviewed. Association between inability to attain adequate voiding function on POD7 and potential predicting factors were evaluated in univariate and multivariate analysis...
April 23, 2018: Journal of Gynecologic Oncology
https://www.readbyqxmd.com/read/29767686/stratification-of-mouse-vaginal-epithelium-1-development-of-3-dimensional-models-in-vitro-with-clonal-cell-lines
#13
Minako Ogawa-Tominaga, Tomohiro Umezu, Tadaaki Nakajima, Yasuhiro Tomooka
The mouse vagina consists of stratified squamous epithelium and stroma and is regulated by ovarian hormones. Vaginal epithelial cells do not stratify, but rather form a monolayer and show an inconsistent responsiveness to ovarian hormones when cultured on plastic dish or matrix. To address the discrepancy between in vivo and in vitro observations, 3 dimensional (3D) co-culture models are developed with clonal vaginal epithelial and stromal cell lines; stromal cells are embedded in collagen gel and epithelial cells are seeded on the gel...
May 14, 2018: Biology of Reproduction
https://www.readbyqxmd.com/read/29762733/the-effect-of-vaginal-microbial-communities-on-colonization-by-staphylococcus-aureus-with-the-gene-for-toxic-shock-syndrome-toxin-1-tsst-1-a-case-control-study
#14
Jacob D Pierson, Melanie A Hansmann, Catherine C Davis, Larry J Forney
Menstrual toxic shock syndrome is associated with vaginal colonization by Staphylococcus aureus strains that encode toxic shock syndrome toxin 1 (tst+). Interestingly, a small proportion of women are colonized by S. aureus tst+ but do not have symptoms of toxic shock syndrome. Here we sought to determine if differences in the species composition of vaginal bacterial communities reflect a differential risk of colonization by S. aureus capable of producing toxic shock syndrome toxin 1 (TSST-1). The composition of vaginal communities of women that were or were not colonized with S...
June 1, 2018: Pathogens and Disease
https://www.readbyqxmd.com/read/29761350/effects-of-gel-volume-on-pharmacokinetics-for-vaginal-and-rectal-applications-of-combination-duogel-iqb4012-a-dual-chamber-dual-drug-hiv-microbicide-gel-in-pigtailed-macaques
#15
Lara E Pereira, Tyana Singletary, Amy Martin, Chuong T Dinh, Frank Deyounks, Angela Holder, Janet McNicholl, Karen W Buckheit, Robert W Buckheit, Anthony Ham, David F Katz, James M Smith
This study evaluated effects of differing gel volumes on pharmacokinetics (PK). IQB4012, a gel containing the non-nucleoside reverse transcriptase inhibitor IQP-0528 and tenofovir (TFV), was applied to the pigtailed macaque vagina and rectum. Vaginal gel volumes (1% loading of both drugs) were 0.5 or 1.5 ml; following wash-out, 1 or 4 ml of gel were then applied rectally. Blood, vaginal, and rectal fluids were collected at 0, 2, 4, and 24 h. Vaginal and rectal tissue biopsies were collected at 4 and 24 h...
May 14, 2018: Drug Delivery and Translational Research
https://www.readbyqxmd.com/read/29758097/persistence-of-polydimethylsiloxane-condom-lubricants
#16
Leah S Tottey, Sally A Coulson, Gerhard E Wevers, Laura Fabian, Heather McClelland, Mickayla Dustin
Polydimethylsiloxane (PDMS) is commonly used to lubricate condoms. The detection of PDMS on swabs from complainants can be used to support an allegation of sexual assault. Previous research has focused on establishing analytical techniques for detecting PDMS. This research examined the persistence of PDMS on the penis, in the vagina, in the mouth, and on skin. The longest PDMS detection times were 20 h on the penis, 35 h in the vagina, and 52 h on skin. PDMS was detected up to 4 h in the mouth if the participant did not eat or drink and up to 9 h if the participant slept...
May 14, 2018: Journal of Forensic Sciences
https://www.readbyqxmd.com/read/29752810/a-prospective-study-of-female-genital-chronic-graft-versus-host-disease-symptoms-signs-diagnosis-and-treatment
#17
Eva Smith Knutsson, Yvonne Björk, Anna-Karin Broman, Lotti Helström, Malin Nicklasson, Mats Brune, Karin Sundfeldt
INTRODUCTION: Female genital chronic graft-versus-host disease (cGvHD) is a complication of allogeneic hematopoietic cell transplantation (alloHCT) for blood malignancies. Unattended inflammation and fibrosis in the vulva and vagina may lead to total vaginal stenosis. The course and treatment of genital cGvHD was observed in this population-based prospective study. MATERIAL AND METHODS: Women (n = 41) receiving alloHCT in 2005-2010 were examined before and at 3, 6, 9, 12, 18, 24, 30, and 36 months post-transplant...
May 12, 2018: Acta Obstetricia et Gynecologica Scandinavica
https://www.readbyqxmd.com/read/29750707/secondary-tumors-of-the-gynecologic-tract-a-clinicopathologic-analysis
#18
Georgia Karpathiou, Celine Chauleur, Sirine Hathroubi, Michel Peoc'h
Although the spread of extragenital tumors to individual female genital tract organs, particularly the ovary, has been much studied, histologic data with regard to secondary tumors involving the whole gynecologic tract are largely lacking. Thus, the aim of the study was to investigate the pathologic and clinical features of these tumors in order to better understand their features. This is a retrospective study of 196 secondary lesions involving the gynecologic tract. The parameters studied were the primary site, its histologic type and grade, the presence of mucous production, the type of secondary involvement, defined as distant metastasis, direct extension or locoregional recurrence, and the time to metastasis...
May 10, 2018: International Journal of Gynecological Pathology
https://www.readbyqxmd.com/read/29747756/veratrum-nigrum-inhibits-the-estrogenic-activity-of-salvia-miltiorrhiza-bunge-in-vivo-and-in-vitro
#19
Ying Xu, Ting Chen, Xin Li, Qu Ya Kun, Jin Na An, Hong Xia Zheng, Yuan Zhao, Zi Jia Zhang, Na Lin
BACKGROUND: As recorded in the 18 incompatible medicaments of Traditional Chinese Medicine theory, the combined use of Salvia miltiorrhiza bunge (SM) and Veratrum nigrum (VN) could induce toxicity and has been prohibited for thousands of years in China. However, the theory has been validated due to lack of evidence. Previous studies have focused on the chemical constituents that are responsible for the toxicity of the two agents. PURPOSE: This study offers preliminary insight into the pharmacodynamics and mechanism of estrogenic activity responsible for their incompatibility...
April 1, 2018: Phytomedicine: International Journal of Phytotherapy and Phytopharmacology
https://www.readbyqxmd.com/read/29747604/barriers-and-facilitators-to-preventive-interventions-for-the-development-of-obstetric-fistulas-among-women-in-sub-saharan-africa-a-systematic-review
#20
Eniya Lufumpa, Lucy Doos, Antje Lindenmeyer
BACKGROUND: Obstetric fistula is a debilitating childbearing injury that results from poorly managed obstructed labour, leading to the development of holes between the vagina and bladder and/or rectum. Effects of this injury are long-lasting, as women become incontinent and are often marginalised from their communities. Despite continuous occurrence of this injury in lower-income countries, it is preventable, as evidenced in high-income countries. This systematic review aims to identify and understand barriers and facilitators to interventions aimed at the prevention of obstetric fistulas in sub-Saharan African women...
May 10, 2018: BMC Pregnancy and Childbirth
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