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Zhou-Li Huang, Jing Dai, Wen-Hua Luo, Xiang-Gui Wang, Jia-Heng Tan, Shuo-Bin Chen, Zhi-Shu Huang
The arginine/glycine-rich region termed the RGG domain is usually found in G-quadruplex (G4)-binding proteins and is im-portant in G4-protein interactions. Studies on the binding mechanism of RGG domains found that small segments (RGG motif) inside the domain contribute greatly to the G4 binding affinity. However, unlike the entire RGG domains that have been broadly explored, the role of the RGG motif remains obscure, with very limited study. Herein, to clarify the role of the RGG motif in G4-protein interactions, we systematically investigated the binding affinity and mode between RGG-motif peptides and G4s...
December 5, 2018: Journal of the American Chemical Society
Mats Hall, Jörgen Wenner, Peter Scherman, Stefan Öberg
OBJECTIVE: Studies of the etiology of intestinal metaplasia (IM) at a normal appearing gastroesophageal junction (GEJ) are conflicting as associations with both H. Pylori (HP) infection and gastroesophageal reflux has been reported. The aim of this study was to investigate whether IM at the GEJ is associated with gastroesophageal reflux or HP infection. MATERIAL AND METHODS: Fifty asymptomatic volunteers and 149 patients with reflux symptoms underwent endoscopy with biopsies obtained from the gastric antrum and the squamocolumnar junction (SCJ)...
December 5, 2018: Scandinavian Journal of Gastroenterology
Andrew K Rudd, Roberto J Brea, Neal K Devaraj
Post-translational S-palmitoylation plays a central role in protein localization, trafficking, stability, aggregation, and cell signaling. Dysregulation of palmitoylation pathways in cells can alter protein function and is the cause of sev-eral diseases. Considering the biological and clinical im-portance of S-palmitoylation, tools for direct, in vivo mod-ulation of this dynamic lipid modification would be ex-tremely valuable. Here, we describe a method for the rapid cleavage of native S-palmitoyl groups from proteins in living cells...
December 5, 2018: Journal of the American Chemical Society
Maciej Ptak, Bartosz Zarychta, Dagmara Stefańska, Aneta Ciupa, Waldeci Paraguassu
We report the synthesis, crystal structure, phonons and luminescence properties of three novel heterometallic metal organic frameworks (MOFs) with perovskite-like topology of the following formulas: [C3H5N2]Na0.5Cr0.5(HCOO)3 (ImNaCr), [C3H5N2]Na0.5Al0.5(HCOO)3 (ImNaAl) and [C3H5N2]Na0.5Al0.475Cr0.025(HCOO)3 (ImNaAlCr with 5 mol% of Cr3+). ImNaCr crystallizes in a monoclinic system (P2/n space group) with one imidazolium cation (Im+) in an asymmetric unit forming six N-HO and four C-HO hydrogen bonds. In contrast to other known heterometallic MOFs, the complete substitution of Cr3+ ions with smaller Al3+ ions leads to a change of the crystal symmetry...
December 5, 2018: Dalton Transactions: An International Journal of Inorganic Chemistry
Jin-Seong Cho, Hyung-Woo Jeon, Min-Ha Kim, The K Vo, Jinsoo Kim, Eung-Jun Park, Young-Im Choi, Hyoshin Lee, Kyung-Hwan Han, Jae-Heung Ko
With the exponential growth of the human population and industrial developments, research on renewable energy resources is required to alleviate environmental and economic impacts caused by the consumption of fossil fuels. In this study, we present a synthetic biological application of a wood forming tissue-specific bicistronic gene expression system to improve both the quantity and quality of woody biomass to minimize undesirable growth penalties. Our transgenic poplars, designed to express both PdGA20ox1 (a GA20-oxidase from Pinus densiflora producing bioactive gibberellin, GA) and PtrMYB221 (a MYB transcription factor negatively regulating lignin biosynthesis) under the developing xylem (DX) tissue-specific promoter (i...
December 5, 2018: Plant Biotechnology Journal
Sara Tookey, Cristina Renzi, Jo Waller, Christian von Wagner, Katriina L Whitaker
BACKGROUND: 'Candidacy' is concerned with the way people consider their eligibility for accessing health services. We used the Candidacy Framework to explore how the doctor-patient relationship can influence perceived eligibility to visit their General Practitioner (GP) among people experiencing cancer alarm symptoms. METHODS: We carried out a secondary analysis of qualitative interviews with 29 women and 33 men, aged ≥50 years experiencing cancer alarm symptoms, recruited through primary care...
December 4, 2018: BMC Health Services Research
An Qin, Dan Ionascu, Jian Liang, Xiao Han, Nicolette O'Connell, Di Yan
BACKGROUND: Advanced clinical applications, such as dose accumulation and adaptive radiation therapy, require deformable image registration (DIR) algorithms capable of voxel-wise accurate mapping of treatment dose or functional imaging. By utilizing a multistage deformable phantom, the authors investigated scenarios where biomechanical refinement method (BM-DIR) may be better than the pure image intensity based deformable registration (IM-DIR). METHODS: The authors developed a biomechanical-model based DIR refinement method (BM-DIR) to refine the deformable vector field (DVF) from any initial intensity-based DIR (IM-DIR)...
December 4, 2018: Radiation Oncology
Adel A Hagag, Mohamed S El Frargy, Amal Ezzat Abd El-Latif
BACKGROUND: Neonatal hypoxic ischemic encephalopathy (HIE) is a potentially devastating disorder associated with significant mortality and long-term morbidity. AIM OF THE STUDY: The aim of this study was to study the role of vitamin D as an adjuvant therapy for management of neonatal HIE. PATIENTS AND METHODS: This study was carried out on 60 neonates with HIE grade II who were diagnosed according to modified Sarnat staging and were divided in to 2 groups: Group I: Included 30 neonates with Sarnat grade II HIE who received single daily oral dose of vitamin D3 (1000 IU) for 2 weeks in addition to daily subcutaneous (SC) human recombinant erythropoietin (2500 IU/kg) for 5 days and IM or IV magnesium sulphate 250 mg/kg within half an hour of birth, and subsequently 125 mg/kg at 24 and 48 hours of life...
December 4, 2018: Endocrine, Metabolic & Immune Disorders Drug Targets
Michael Selgrad, Jan Bornschein, Arne Kandulski, Jochen Weigt, Albert Roessner, Thomas Wex, Peter Malfertheiner
BACKGROUND: Our aim was to evaluate the feasibility of a serological assessment of gastric cancer risk in patients undergoing colonoscopy in countries with low-to-moderate incidence rates. METHODS: Serum samples were prospectively collected from 453 patients (>50 years old) undergoing colonoscopies. Of these, 279 (61.6%) also underwent gastroscopy to correlate the results for serum pepsinogen I and II (sPG-I and sPG-II), sPG-I/II ratio, and anti- H. pylori antibodies with gastric histopathology findings (graded according to the updated Sydney classification and the Operative Link of Gastritis Assessment (OLGA) and the Operative Link for Gastric Intestinal Metaplasia assessment (OLGIM) systems)...
December 3, 2018: International Journal of Molecular Sciences
Adla Bakri Hassan, Reham Fathi Hozayen, Raed Aqshan Alotaibi, Yasin Ibrahim Tayem
Studies carried out assessing the effect of different doses of cholecalciferol (vitamin D3) on correcting serum 25-hydroxyvitamin D deficiency in healthy adults are limited and review studies are lacking. Moreover, the maintenance dose and its duration offered by these few studies are inconsistent. We performed a systematic review of randomized clinical controlled trials (RCTs) that assessed the effect of different doses of vitamin D3 on serum 25(OH)D in healthy adults. PubMed database was searched from 2010 to 2018 using the following search terms: "vitamin D deficiency", "Cholecalciferol", "vitamin D3 dose", "vitamin D supplement", "vitamin D therapy"...
November 30, 2018: Cellular and Molecular Biology
Shou-Wu Lee, Han-Chung Lien, Chi-Sen Chang, Ming-Xian Lin, Chung-Hsin Chang, Chung-Wang Ko
AIM: To investigate the benefits of the Seattle protocol in the diagnosis of Chinese individuals with Barrett's esophagus. METHODS: Subjects enrolled were patients from one center with endoscopically-suspected esophageal metaplasia. These patients first received narrow-band imaging-targeted biopsy, and later, the Seattle protocol-guided biopsy, within a period from October 2012 to December 2014. Those cases without initial pathologic patterns of intestinal metaplasia (IM) and then appearance or loss of IM tissue were designated as Group A or B, respectively...
November 26, 2018: World Journal of Clinical Cases
Hee-Jin Kim, Tae-Wook Kang, Keeok Haam, Mirang Kim, Seon-Kyu Kim, Seon-Young Kim, Sang-Il Lee, Kyu-Sang Song, Hyun-Yong Jeong, Yong Sung Kim
DNA methylation is a regulatory mechanism in epigenetics that is frequently altered during human carcinogenesis. To detect critical methylation events associated with gastric cancer (GC), we compared three DNA methylomes from gastric mucosa (GM), intestinal metaplasia (IM), and gastric tumor (GT) cells that were microscopically dissected from an intestinal-type early gastric cancer (EGC) using methylated DNA binding domain sequencing (MBD-seq) and reduced representation bisulfite sequencing (RRBS) analysis...
December 3, 2018: Experimental & Molecular Medicine
KaHo Leung, Kasturi Chakraborty, Anand Saminathan, Yamuna Krishnan
Lysosomes are multifunctional, subcellular organelles with roles in plasma membrane repair, autophagy, pathogen degradation and nutrient sensing. Dysfunctional lysosomes underlie Alzheimer's disease, Parkinson's disease and rare lysosomal storage diseases, but their contributions to these pathophysiologies are unclear. Live imaging has revealed lysosome subpopulations with different physical characteristics including dynamics, morphology or cellular localization. Here, we chemically resolve lysosome subpopulations using a DNA-based combination reporter that quantitatively images pH and chloride simultaneously in the same lysosome while retaining single-lysosome information in live cells...
December 3, 2018: Nature Nanotechnology
Sho Shibata, Kensuke Miyake, Tomoya Tateishi, Soichiro Yoshikawa, Yoshinori Yamanishi, Yasunari Miyazaki, Naohiko Inase, Hajime Karasuyama
Chronic obstructive pulmonary disease (COPD) is a leading cause of morbidity and mortality worldwide. It has generally been considered a non-Th2-type lung disorder, characterized by progressive airflow limitation with inflammation and emphysema, but its cellular and molecular mechanism remains ill defined, compared with that of asthma characterized by reversible airway obstruction. Here we show a previously unappreciated role for basophils at the initiation phase of emphysema formation in an elastase-induced murine model of COPD in that basophils represent less than 1% of lung-infiltrating cells...
December 3, 2018: Proceedings of the National Academy of Sciences of the United States of America
Maneesh Kumar Ahirwar, Mukund Amritrao Kataktalware, Heartwin Amaladhas Pushpadass, Sakthivel Jeyakumar, Soumitra Jash, Sayyad Nazar, Letha Devi G, John P Kastelic, Kerekoppa Puttaiah Ramesha
The objective was to use scrotal infrared digital thermography to evaluate effects of thermal stress on semen quality of Murrah buffalo (Bubalus bubalis) breeding bulls. Ejaculates from 109 Murrah bulls maintained at three semen stations were evaluated for: ejaculate volume, sperm concentration (SCON), initial motility (IM), percent live sperm (LIVE), acrosome integrity of fresh semen (AIFS), plasma membrane integrity of fresh semen (PMIFS), head abnormalities of fresh semen (HAFS), midpiece abnormalities of fresh semen (MPAFS), tail abnormalities of fresh semen (TAFS), post-thaw motility (PTM), acrosome integrity of post-thawed semen (AIPT) and plasma membrane integrity of post-thawed semen (PMIPT)...
December 2018: Journal of Thermal Biology
Yu-Mi Im, Tae-Jin Yun, Il-Young Yoo, Sanghee Kim, Juhye Jin, Sue Kim
BACKGROUND: Prenatal diagnosis of fetal congenital heart disease (CHD) is becoming widely available but there is a lack of understanding on such expectant mothers' experiences during pregnancy. This was the first study to investigate the pregnancy experience of Korean mothers with a prenatal fetal diagnosis of CHD. METHODS: In-depth interviews were conducted with 12 mothers regarding their child's prenatal diagnosis of CHD and the adaptive processes during pregnancy...
December 3, 2018: BMC Pregnancy and Childbirth
Peter Sona, Jong Hui Hong, Sunho Lee, Byong Joon Kim, Woon-Young Hong, Jongcheol Jung, Han-Na Kim, Hyung-Lae Kim, David Christopher, Laurent Herviou, Young Hwan Im, Kwee-Yum Lee, Tae Soon Kim, Jongsun Jung
BACKGROUND: The use of whole genome sequence has increased recently with rapid progression of next-generation sequencing (NGS) technologies. However, storing raw sequence reads to perform large-scale genome analysis pose hardware challenges. Despite advancement in genome analytic platforms, efficient approaches remain relevant especially as applied to the human genome. In this study, an Integrated Genome Sizing (IGS) approach is adopted to speed up multiple whole genome analysis in high-performance computing (HPC) environment...
December 3, 2018: BMC Bioinformatics
Md Mamun Monir, Mita Khatun, Md Nurul Haque Mollah
Interval mapping approaches have been playing significant role for quantitative trait locus (QTL) mapping to discover genetic architecture of diseases or traits with molecular markers. Composite interval mapping (CIM) is one of the superior approaches of the interval mapping for discovering both linked and unlinked putative QTL positions. However, estimators of this approach are not robust against phenotypic outliers. As a result, it fails to detect true QTL positions in presence of outliers. In this study, we investigated the performance of β-Composite Interval Mapping (BetaCIM) for detecting both linked and unlinked important QTLs positions from the robustness points of views...
2018: PloS One
Kevin J Endres, Ting-Zheng Xie, Sourav Chakraborty, Chad Hoopingarner, Chrys Wesdemiotis
Ion mobility-mass spectrometry (IM-MS) allows the separation of isomeric and isobaric species on the basis of their size, shape, and charge. The fast separation timescale (ms) and high sensitivity of these measurements make IM-MS an ideally suitable method for monitoring changes in macromolecular structure, such as those occurring in interconverting terpyridine-based metallosupramolecular self-assemblies. IM-MS is used to verify the elemental composition (size) and architecture (shape) of the self-assembled products...
November 30, 2018: Macromolecular Rapid Communications
Lingquan Wang, Wei Xu, Xuexin Yao, Chao Yan, Chen Li, Zhenggang Zhu, Wentao Liu, Min Yan
OBJECTIVE: To investigate the clinical characteristics and prognostic factors of reoperation patients with postoperative recurrence or metastasis of gastrointestinal stromal tumor (GIST). METHODS: A retrospective case-control study was performed on the clinical data of 31 patients with GIST who had recurrence or metastasis after the first surgery and underwent one or more operations again from February 2003 to January 2016 at Ruijin Hospital, Shanghai Jiaotong University School of Medicine...
November 25, 2018: Zhonghua Wei Chang Wai Ke za Zhi, Chinese Journal of Gastrointestinal Surgery
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