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Calcium dependent protein kinase

Sun-Hye Choi, Hyeon-Joong Kim, Hee-Jung Cho, Sang-Deuk Park, Na-Eun Lee, Sung-Hee Hwang, Ik-Hyun Cho, Hongik Hwang, Hyewhon Rhim, Hyoung-Chun Kim, Seung-Yeol Nah
Astrocytes are a unique brain cell-storing glycogen and express lysophosphatidic acid (LPA) receptors. Gintonin is a ginseng-derived exogenous G protein-coupled LPA receptor ligand. Accumulating evidence shows that astrocytes serve as an energy supplier to neurons through astrocytic glycogenolysis under physiological and pathophysiological conditions. However, little is known about the relationships between LPA receptors and astrocytic glycogenolysis or about the roles of LPA receptors in hypoxia and re-oxygenation stresses...
August 16, 2018: Molecular Neurobiology
Xue Zhu, Ke Wang, Fanfan Zhou, Ling Zhu
Abnormal accumulation of the free-form all-trans-retinal (atRAL), a major intermediate of human visual cycle, is considered to be a key cause of retinal pigment epithelial (RPE) dysfunction in the pathogenesis of retinal degenerative diseases such as age-related macular degeneration (AMD). Paeoniflorin (PF), a monoterpene glucoside isolated from Paeonia lactiflora Pall., has been used in clinical treatment of retinal degenerative diseases in China for several years; however, the underlying mechanism remains unclear...
August 16, 2018: Archives of Pharmacal Research
Lucie Potuckova, Lubica Draberova, Ivana Halova, Tomas Paulenda, Petr Draber
C-terminal Src kinase (CSK) is a major negative regulator of Src family tyrosine kinases (SFKs) that play critical roles in immunoreceptor signaling. CSK is brought in contiguity to the plasma membrane-bound SFKs via binding to transmembrane adaptor PAG, also known as CSK-binding protein. The recent finding that PAG can function as a positive regulator of the high-affinity IgE receptor (FcεRI)-mediated mast cell signaling suggested that PAG and CSK have some non-overlapping regulatory functions in mast cell activation...
2018: Frontiers in Immunology
Kellie E Cunningham, Elizabeth A Novak, Garret Vincent, Vei Shaun Siow, Brian D Griffith, Sarangarajan Ranganathan, Matthew R Rosengart, Jon D Piganelli, Kevin P Mollen
The incidence and prevalence of inflammatory bowel disease (IBD) are increasing worldwide. IBD is known to be multifactorial, but inflammatory signaling within the intestinal epithelium and a subsequent failure of the intestinal epithelial barrier have been shown to play essential roles in disease pathogenesis. CaMKIV is a multifunctional protein kinase associated with inflammation and cell cycle regulation. CaMKIV has been extensively studied in autoimmune diseases, but a role in idiopathic intestinal inflammation has not been described...
August 16, 2018: FASEB Journal: Official Publication of the Federation of American Societies for Experimental Biology
Sara R Oliveira, Cláudia Figueiredo-Pereira, Carlos B Duarte, Helena L A Vieira
Carbon monoxide (CO) is an endogenous gasotransmitter that limits inflammation and prevents apoptosis in several tissues, including the brain. Low concentrations of CO are cytoprotective in astrocytes, neurons, and microglia, but the underlying molecular mechanisms remain poorly understood. This work aims at identification of alterations in gene expression conferred by CO in primary cultures of cortical astrocytes, for further disclosure of the molecular mechanism of action of the gasotransmitter. Astrocytes were treated with the CO-releasing molecule CORM-A1 for 40 min, and transcriptional changes were analyzed using RNASeq...
August 13, 2018: Molecular Neurobiology
John M Haynes, James N Selby, Isaiah Pl Abad, Teresa Vandekolk, Joan K Ho, Wai-Ling Lieuw, Katie Leach, Judith Savige, Sheetal Saini, Craig Fisher, Sharon Ricardo
Renal podocyte survival depends upon the dynamic regulation of a complex cell architecture that links glomerular basement membrane to integrins, ion channels and receptors. Alport syndrome is a heritable chronic kidney disease where mutations in α3, α4 or α5 collagen genes promote podocyte death. In rodent models of renal failure, activation of the calcium sensing receptor (CaSR) can protect podocytes from stress related death. In this study we assess CaSR function in podocyte-like cells from induced-pluripotent stem cells derived from two patients with Alport Syndrome (AS1 & AS2), a renal disease free individual (NHMC) and an immortalized podocyte-like (HIP) cell line...
August 13, 2018: Journal of Pharmacology and Experimental Therapeutics
Tai-Fei Yu, Wan-Ying Zhao, Jin-Dong Fu, Yong-Wei Liu, Ming Chen, Yong-Bin Zhou, You-Zhi Ma, Zhao-Shi Xu, Ya-Jun Xi
Plant calcium-dependent protein kinases (CDPKs) were reported to play important roles in plant resistance to abiotic stress. Foxtail millet cultivation "H138" was used for RNA-seq analysis. The data from drought-induced de novo transcriptomic sequences of foxtail millet showed that CDPKs were up- or down-regulated by drought to different degrees. In this study, 29 foxtail millet CDPKs were classified into four subgroups. These genes were unevenly distributed on nine foxtail millet chromosomes, and chromosomes 2, 3, and 9 contained the most SiCDPK members...
2018: Frontiers in Plant Science
Mara Simopoulou, Konstantinos Sfakianoudis, Nikolaos Antoniou, Evangelos Maziotis, Anna Rapani, Panagiotis Bakas, George Anifandis, Theodoros Kalampokas, Stamatis Bolaris, Agni Pantou, Konstantinos Pantos, Michael Koutsilieris
Assisted reproductive technology has evolved tremendously since the emergence of in vitro fertilization (IVF). In the course of the recent decade, there have been significant efforts in order to minimize multiple gestations, while improving percentages of singleton pregnancies and offering individualized services in IVF, in line with the trend of personalized medicine. Patients as well as clinicians and the entire IVF team benefit majorly from 'knowing what to expect' from an IVF cycle. Hereby, the question that has emerged is to what extent prognosis could facilitate toward the achievement of the above goal...
August 8, 2018: Systems Biology in Reproductive Medicine
Zhujin Song, Hui Chen, Wen Xu, Shengbing Wu, Guoqi Zhu
Extinction of fear-memory is essential for emotional and mental changes. However, the mechanisms underlying extinction of fear-memory are largely unknown. Calpain is a type of calcium-dependent protease that plays a critical role in memory consolidation and reconsolidation. Whether calpain functions in extinction of fear-memory is unknown, as are the molecular mechanisms. In this study, we investigated the pivotal role of calpain in extinction of fear-memory in mice, and assessed its mechanism. Conditioned stimulation/unconditioned stimulation-conditioned stimulation paradigms combined with pharmacological methods were employed to evaluate the action of calpain in memory extinction...
August 4, 2018: Neurobiology of Learning and Memory
Zhijun Liu, Yiju Wei, Lei Zhang, Patricia P Yee, Martin Johnson, Xuexin Zhang, Melissa Gulley, Jennifer M Atkinson, Mohamed Trebak, Hong-Gang Wang, Wei Li
Glioblastomas (GBM) are the most aggressive brain cancers without effective therapeutics. The Hippo pathway transcriptional coactivators YAP/TAZ were implicated as drivers in GBM progression and could be therapeutic targets. Here we found in an unbiased screen of 1650 compounds that amlodipine is able to inhibit survival of GBM cells by suppressing YAP/TAZ activities. Instead of its known function as an L-type calcium channel blocker, we found that amlodipine is able to activate Ca2+ entry by enhancing store-operated Ca2+ entry (SOCE)...
August 6, 2018: Oncogene
Martin Flück, Paola Valdivieso, Severin Ruoss, Brigitte von Rechenberg, Mario C Benn, Dominik C Meyer, Karl Wieser, Christian Gerber
INTRODUCTION: We evaluated the contribution of denervation-related molecular processes to rotator cuff muscle degeneration after tendon release. METHODS: We assessed the levels of myogenic (myogenin and myogenic differentiation factor [myoD]) and pro-adipogenic (peroxisome proliferator-activated receptor gamma) transcription factors; the denervation-associated proteins tenascin-C, laminin-2, and calcium/calmodulin dependent kinase II (CaMKII); and cellular alterations in sheep after infraspinatus tenotomy (TEN), suprascapular neurectomy (NEU), or both (TEN-NEU)...
August 2, 2018: Muscle & Nerve
Dongun Lee, Sang A Lee, Dong M Shin, Jeong H Hong
Secretory glands including salivary glands by many hormonal inputs produce and secrete biological fluids determined by variety of ion transporters. Spinophilin is a multifunctional scaffolding protein, which involved in receptor signaling and regulation of anion exchangers AE2 activity. We found that spinophilin expressed in salivary glands. The role of salivary spinophilin on the modulation of chloride/bicarbonate exchange remains unknown. The spinophilin enhanced AE2 activity and associated with a STE20/SPS1-related kinase and showed an additive effect on the modulation of the activity of AE2...
2018: Frontiers in Physiology
Leandro Royer, Josiah J Herzog, Katelyn Kenny, Boriana Tzvetkova, Jesse C Cochrane, Michael T Marr, Suzanne Paradis
Ca2+ /calmodulin-dependent protein kinase II (CaMKII) is a well-characterized, abundant protein kinase that regulates a diverse set of functions in a tissue-specific manner. For example, in heart muscle, CaMKII regulates Ca2+ homeostasis, while in neurons, CaMKII regulates activity-dependent dendritic remodeling and long-term potentiation (LTP), a neurobiological correlate of learning and memory. Previously, we identified the GTPase Rem2 as a critical regulator of dendrite branching and homeostatic plasticity in the vertebrate nervous system...
August 2, 2018: Journal of Biological Chemistry
H T H Nguyen, F Bouteau, C Mazars, M Kuse, T Kawano
Changes in Ca2+ concentrations in cytosol ([Ca2+ ]C ) or nucleus ([Ca2+ ]N ) may play some vital roles in plants under hypoosmotic shock (Hypo-OS). Here, we observed that Hypo-OS induces biphasic increases in [Ca2+ ]C and [Ca2+ ]N in two tobacco cell lines (BY-2) expressing apoaequorin either in the cytosol or in the nucleus. Both [Ca2+ ]C and [Ca2+ ]N were sensitively modulated by the inhibitors of calmodulin and protein kinases, supporting the view that calmodulin suppresses the 1st peaks and and protein kinases enhance 2nd peaks in [Ca2+ ]C and [Ca2+ ]N ...
August 1, 2018: Plant Signaling & Behavior
Yingxiao Fu, Dequn Niu, Wenfang Su, Qingling Yang, Wenrui Wang, Baoding Tang, Zhongwen Li, Ding Zhang, Yingji Mao, Chuang Li, Xue Li, Shihao Ye, Xu Su, Fanyuan Xu, Xuemin Sun, Changjie Chen
The aim of the present study was to determine the effects of the Ca2+/calmodulin‑dependent protein kinase pathway inhibitor KN93 on osteoclastogenesis. RAW264.7 cells were incubated with macrophage colony‑stimulating factor (M‑CSF) + receptor activator of nuclear factor kappa‑light‑chain‑enhancer of activated B cells ligand (RANKL) to stimulate osteoclastogenesis and then treated with 10 µM KN93. The methods included tartrate‑resistant acid phosphatase (TRAP) staining, bone resorption activity assays, filamentous (F)‑actin staining, determination of intracellular calcium ([Ca2+]i) levels, monitoring of osteoclast‑specific gene expression levels and measurement of key transcription factors protein levels...
July 25, 2018: International Journal of Molecular Medicine
Ling-Zhi Xu, Bing-Qiu Li, Jian-Ping Jia
Alzheimer's disease (AD) is the most common neurodegenerative disease and seriously damages the health of elderly population. Clinical drug research targeting at classic pathology hallmarks, such as amyloid-β (Aβ) and tau protein, failed to achieve effective cognitive improvement, suggesting that the pathogenesis of AD is much complicated, and there are still other unknown and undetermined important factors. Death-associated protein kinase 1 (DAPK1) is a calcium/calmodulin-dependent serine/threonine kinase that plays an important role in various neuronal injury models...
July 31, 2018: Molecular Neurobiology
Tamara P Martin, Claire McCluskey, Margaret R Cunningham, James Beattie, Andrew Paul, Susan Currie
Calcium/calmodulin dependent protein kinase IIδ (CaMKIIδ) acts as a molecular switch regulating cardiovascular Ca2+ handling and contractility in health and disease. Activation of CaMKIIδ is also known to regulate cardiovascular inflammation and is reported to be required for pro-inflammatory NF-κB signalling. In this study the aim was to characterise how CaMKIIδ interacts with and modulates NF-κB signalling and whether this interaction exists in non-contractile cells of the heart. Recombinant or purified CaMKIIδ and the individual inhibitory -κB kinase (IKK) proteins of the NF-κB signalling pathway were used in autoradiography and Surface Plasmon Resonance (SPR) to explore potential interactions between both components...
July 27, 2018: Cellular Signalling
Agnieszka Michalak, Karolina Pekala, Barbara Budzynska, Marta Kruk-Slomka, Grazyna Biala
Drugs of abuse trigger a very specific type of memory called state-dependent memory (SDM). Both memory process and drug addiction are underlain by neuroplasticity, which depends on calcium concentration and protein kinase activity. Within the scope of this study was to evaluate the impact of verapamil, an L-type voltage-gated calcium channel (VGCC) blocker, and SL-327, a selective MAPK/ERK kinase inhibitor, on morphine and ethanol SDM including the cross effects between these drugs with an additional influence of nicotine...
September 5, 2018: European Journal of Pharmacology
Salome Kylarova, Katarina Psenakova, Petr Herman, Veronika Obsilova, Tomas Obsil
BACKGROUND: Calcium/calmodulin-dependent protein kinase kinase 2 (CaMKK2), a member of the Ca⁠2+ /calmodulin-dependent kinase (CaMK) family, functions as an upstream activator of CaMKI, CaMKIV and AMP-activated protein kinase. Thus, CaMKK2 is involved in the regulation of several key physiological and pathophysiological processes. Previous studies have suggested that Ca2+ /CaM binding may cause unique conformational changes in the CaMKKs compared with other CaMKs. However, the underlying mechanistic details remain unclear...
July 24, 2018: Biochimica et Biophysica Acta
Anam Beg, Faez Iqbal Khan, Kevin A Lobb, Asimul Islam, Faizan Ahmad, Imtaiyaz Hassan
Calcium/calmodulin-dependent protein kinase IV (CAMKIV) is associated with many diseases including cancer and neurodegenerative disorders, and thus being considered as a potential drug target. Here, we have employed the knowledge of three-dimensional structure of CAMKIV to identify new inhibitors for possible therapeutic intervention. We have employed virtual high throughput screening of 12500 natural compounds of Zinc database to screen the best possible inhibitors of CAMKIV. Subsequently 40 compounds which showed significant docking scores (-11...
July 25, 2018: Journal of Biomolecular Structure & Dynamics
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