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Calcium dependent protein kinase

Benny Zhitomirsky, Anna Yunaev, Roman Kreiserman, Ariel Kaplan, Michal Stark, Yehuda G Assaraf
Transcription factor EB (TFEB) is a master transcriptional regulator playing a key role in lysosomal biogenesis, autophagy and lysosomal exocytosis. TFEB activity is inhibited following its phosphorylation by mammalian target of rapamycin complex 1 (mTORC1) on the surface of the lysosome. Phosphorylated TFEB is bound by 14-3-3 proteins, resulting in its cytoplasmic retention in an inactive state. It was suggested that the calcium-dependent phosphatase calcineurin is responsible for dephosphorylation and subsequent activation of TFEB under conditions of lysosomal stress...
December 13, 2018: Cell Death & Disease
Seonung Lim, Wonwoo Lee, Doo Suk Lee, In-Jeong Nam, Nayoung Yun, Yoonseon Jeong, Taewoong Rho, Sunyoung Kim
Benign prostatic hyperplasia (BPH) is a common disease in the elderly male population throughout the world. Among other factors, androgen dysregulation has been known to play major roles in its pathogenesis. HX109 is a botanical formulation prepared from a mixture of Taraxacum officinale , Cuscuta australis , and Nelumbo nucifera , which have traditionally been used-usually along with other plants-to treat urinary diseases. An ethanol extract was prepared from a mixture of these three plants, and its quality was controlled through cell-based bioassays and by quantification of several marker compounds by high-performance liquid chromatography (HPLC)...
December 7, 2018: Nutrients
Kai Li, Wen Li, Hongqiang Yin, Yuen-Ki Cheong, Guogang Ren, Zhuo Yang
Chronic hypoxic damage is one of the most common pathogenic factors that can cause neurodegenerative disorder in most cases. Etidronate (Eti) is one of the best-known earlier-generations of bisphosphonate derivatives for the treatment of bone-loss related diseases. Building on the preceding study of our laboratory, we found that Eti showed neuroprotective effects against 2-vessel occlusion induced vascular dementia (VD) in rats. Therefore, in this study, we attempted to elucidate the mechanism of action, which Eti protected cells from chronic hypoxic damage caused by CoCl2 in SH-SY5Y cells in vitro...
December 11, 2018: Neurochemical Research
Yingliang Wei, Zhaofeng Jin, He Zhang, Shang Piao, Jinghan Lu, Lunhao Bai
BACKGROUND/AIMS: Chondrocyte apoptosis is a central pathological feature of cartilage in osteoarthritis (OA). Accumulating evidence suggests that calcium ions (Ca2+) are an important regulator of apoptosis. Previously, we reported that the transient receptor potential channel vanilloid (TRPV5) is upregulated in monoiodoacetic acid (MIA)-induced OA articular cartilage. METHODS: The protein levels of TRPV5, phosphorylated Ca2+/calmodulin-dependent kinase II (p-CaMKII), and total CaMKII were detected in vivo using western blotting techniques...
2018: Cellular Physiology and Biochemistry
Yue Xu, Oliver Berkowitz, Reena Narsai, Inge De Clercq, Michelle Hooi, Bulone Vincent, Frank Van Breusegem, James Whelan, Yan Wang
Plants respond to short- and long-term mechanical stimuli, via altered transcript abundance and growth respectively. Jasmonate, gibberellic acid and calcium have been implicated in mediating responses to mechanical stimuli. Previously it was shown that the transcript abundance for the outer mitochondrial membrane protein of 66 kDa (OM66), is induced several-fold after 30 min in response to touch. Thus, the impact of mitochondrial function on the response to mechanical stimulation by touch at 30 min was investigated...
December 8, 2018: Plant Journal: for Cell and Molecular Biology
Dong-Hua Chen, Hui-Ping Liu, Chun-Long Li
In this manuscript, we demonstrated the negative role of CPK9 in stomatal ABA signaling, and both CPK9 and CPK33 for accurate guard cell function was explored via cpk9/cpk33 double mutants' phenotype. Abscisic acid (ABA) can inhibit stomatal opening and promote stomatal closure by regulating ion channel activity in guard cell membranes. As an important second messenger, calcium (Ca2+ ) is essentially needed in ABA regulation of stomatal movement. Calcium-dependent protein kinases (CDPKs) have been proposed to contribute to central Ca2+ signal transduction in plants...
December 8, 2018: Plant Molecular Biology
Taiki Shimizu, Kenta Kanai, Yui Sugawara, Chiyoko Uchida, Takafumi Uchida
Calcium/calmodulin-dependent protein kinase II (CaMKII) is abundant in the brain and functions as a mediator of calcium signaling. We found that the relative activity of CaMKII was significantly lower in the WT mouse brains than in the Pin1-/- mouse brains. Pin1 binds to phosphorylated CaMKII and weakens its activity. For this reason, the phosphorylation level of tau in the presence of Pin1 is lower than that in the absence of Pin1, and microtubule polymerization is not downregulated by CaMKII when Pin1 is present...
2018: Frontiers in Pharmacology
Shigeki Moriguchi, Satomi Kita, Takahiro Iwamoto, Kohji Fukunaga
Na+ /Ca2+ exchanger (NCX) is mainly expressed in the plasma membrane and mediates electrogenical exchange of one Ca2+ for three Na+ , depending on the electrochemical gradients across the plasma membrane. NCX has three different isoforms (NCX1, NCX2, NCX3) encoded by distinct genes in mammals. Here, we report that NCX2 and NCX3 protein levels are relatively reduced in hippocampal CA1 of Alzheimer's disease model mice. Likewise, NCX2+/- or NCX3+/- mice exhibited impaired hippocampal LTP and memory-related behaviors...
2018: Nihon Yakurigaku Zasshi. Folia Pharmacologica Japonica
Rui Kamada, Hisashi Yokoshiki, Hirofumi Mitsuyama, Masaya Watanabe, Kazuya Mizukami, Taro Tenma, Masayuki Takahashi, Shingo Takada, Toshihisa Anzai
Sustained ventricular arrhythmias (SVAs) lead to sudden cardiac death, for which β- adrenoreceptor blockers are effective. We hypothesized that electrophysiological changes and arrhythmias by β- adrenoreceptor stimulation are crucially related to activation of small-conductance calcium-activated potassium (SK) channels via the increase in Ca2+ / calmodulin-dependent protein kinase II (CaMKII) activity. We used normotensive Wistar-Kyoto (WKY) rats and spontaneous hypertensive rats (SHRs). The latter served as a model of left ventricular hypertrophy...
December 7, 2018: European Journal of Pharmacology
Qiqi Song, Cuiqin Fan, Peng Wang, Ye Li, Mu Yang, Shu Yan Yu
BACKGROUND: Neuroinflammation has recently emerged as a critical risk factor in the pathophysiology of depression. However, the underlying molecular mechanisms and the development of novel therapeutic strategies as means to target these inflammatory pathways for use in the treatment of depression remain unresolved. In the present study, we aimed to investigate the molecular events of neuroinflammation as related to its induction of depression-like behaviors. METHODS: Chronic unpredictable mild stress (CUMS) or lipopolysaccharide (LPS) was used to induce depression-like behaviors in rats...
December 8, 2018: Journal of Neuroinflammation
Pojeong Park, Heather Kang, Thomas M Sanderson, Zuner A Bortolotto, John Georgiou, Min Zhuo, Bong-Kiun Kaang, Graham L Collingridge
Long-term potentiation (LTP) at hippocampal CA1 synapses is classically triggered by the synaptic activation of NMDA receptors (NMDARs). More recently, it has been shown that calcium-permeable (CP) AMPA receptors (AMPARs) can also trigger synaptic plasticity at these synapses. Here, we review this literature with a focus on recent evidence that CP-AMPARs are critical for the induction of the protein kinase A (PKA)- and protein synthesis-dependent component of LTP.
2018: Frontiers in Synaptic Neuroscience
Cuiping Liu, Guoli Shao, Yirong Lu, Minmin Xue, Fenfen Liang, Zhenshu Zhang, Lan Bai
BACKGROUND/AIMS: Parathyroid hormone-related protein (PTHrP) is implicated in regulating calcium homeostasis in vertebrates, including sea bream, chick, and mammals. However, the molecular mechanism underlying the function of PTHrP in regulating calcium transport is still not fully investigated. This study aimed to investigate the effect of PTHrP on the calcium uptake and its underlying molecular mechanism in rat enterocytes. METHODS: The rat intestinal epithelial cell line (IEC-6) was used...
2018: Cellular Physiology and Biochemistry
Wesley L Bollinger, Nadia Sial, Ken Dawson-Scully
A cGMP-dependent protein kinase (PKG) has previously been shown to regulate synaptic transmission at the Drosophila neuromuscular junction (NMJ) during acute oxidative stress, potentially through modulation of downstream K+ channel kinetics; however, the specific K+ channels through which PKG functions remains unclear. In this study, we hypothesized that PKG may be acting on calcium-activated large-conductance Slo K+ channels, or BK channels. We found that genetic elimination and pharmacological inhibition of BK channel conductance increases synaptic transmission tolerance to acute H2 O2 -induced oxidative stress...
September 2018: Journal of Neurogenetics
Feng Cao, Zikai Zhou, Sammy Cai, Wei Xie, Zhengping Jia
The GluA2 subunit of AMPA glutamate receptors (AMPARs) has been shown to be critical for the expression of NMDA receptor (NMDAR)-dependent long-term depression (LTD). However, in young GluA2 knockout (KO) mice, this form of LTD can still be induced in the hippocampus, suggesting that LTD mechanisms may be modified in the presence of GluA2-lacking, Ca2+ permeable AMPARs. In this study, we examined LTD at the CA1 synapse in GluA2 KO mice by using several well-established inhibitory peptides known to block LTD in wild type (WT) rodents...
2018: Frontiers in Synaptic Neuroscience
Anne Creon, Inokentijs Josts, Stephan Niebling, Nils Huse, Henning Tidow
Calmodulin (CaM) is a very conserved, ubiquitous, eukaryotic protein that binds four Ca2+ ions with high affinity. It acts as a calcium sensor by translating Ca2+ signals into cellular processes such as metabolism, inflammation, immune response, memory, and muscle contraction. Calcium binding to CaM leads to conformational changes that enable Ca2+ /CaM to recognize and bind various target proteins with high affinity. The binding mode and binding partners of CaM are very diverse, and a consensus binding sequence is lacking...
November 2018: Structural Dynamics (Melville, N.Y.)
Shrawan Kumar Mishra, Daniel J Stephenson, Charles E Chalfant, Rhoderick E Brown
Human GLTP on chromosome 12 (locus 12q24.11) encodes a 24 kD amphitropic lipid transfer protein (GLTP) that mediates glycosphingolipid (GSL) intermembrane trafficking and regulates GSL homeostatic levels within cells. Herein, we provide evidence that GLTP overexpression inhibits the growth of human colon carcinoma cells (HT-29; HCT-116), but spares normal colonic cells (CCD-18Co). Mechanistic studies reveal that GLTP overexpression arrested the cell cycle at the G1/S checkpoint via upregulation of cyclin-dependent kinase inhibitor-1B (Kip1/p27) and cyclin-dependent kinase inhibitor 1A (Cip1/p21) at the protein and mRNA levels, and downregulation of cyclin-dependent kinase-2 (CDK2), cyclin-dependent kinase-4 (CDK4), cyclin E and cyclin D1 protein levels...
November 22, 2018: Biochimica et biophysica acta. Molecular and cell biology of lipids
Dorota Dymkowska, Beata Drabarek, Anna Michalik, Natalia Nowak, Krzysztof Zabłocki
Previously we showed that a mild stimulation of EA.hy926 cells with tumour necrosis factor alpha (TNFα) activated mitochondrial biogenesis, probably as a mechanism preventing cell death. This was accompanied by an increased phosphorylation of eNOS and elevation of NO release. The aim of the present study was to explain the biochemical basis of this effect. Our results indicate that eNOS is the only enzyme catalysing NO generation in EA.hy926 cells, and TNFα stimulates its activity by activating AMP-activated protein kinase (AMPK)...
November 22, 2018: International Journal of Biochemistry & Cell Biology
Alexander Panossian, Ean-Jeong Seo, Thomas Efferth
INTRODUCTION: Adaptogens are natural compounds or plant extracts that increase adaptability and survival of organisms under stress. Adaptogens stimulate cellular and organismal defense systems by activating intracellular and extracellular signaling pathways and expression of stress-activated proteins and neuropeptides. The effects adaptogens on mediators of adaptive stress response and longevity signaling pathways have been reported, but their stress-protective mechanisms are still not fully understood...
November 15, 2018: Phytomedicine: International Journal of Phytotherapy and Phytopharmacology
Tomasz Misztal, Tomasz Rusak, Justyna Brańska-Januszewska, Marta Gąsowska, Beata Szynaka, Agata Gołaszewska, Marta Bruczko, Marian Tomasiak
This study was undertaken to establish the presence and the role of aquaporins (AQPs) in human platelets. Immunodetection with polyclonal antibodies and fluorescent microscopy suggest the presence of AQP isoforms - 0-7 and 9-12 - localized (in resting platelets) in the plasma membrane and in the dense and alpha granules. In thrombin- or monensin-treated platelets, the granules' AQPs become visible in the whole cell body, indicating the granules' swelling. In our studies on the role of AQPs in platelet responses we used tetrachloroauric acid (HAuCl4), a classical water channel blocker...
November 22, 2018: Acta Biochimica Polonica
Sarah Rouhana, Charlotte Farah, Jerome Roy, Amanda Finan, Glaucy Rodrigues de Araujo, Patrice Bideaux, Valérie Scheuermann, Youakim Saliba, Cyril Reboul, Olivier Cazorla, Franck Aimond, Sylvain Richard, Jérôme Thireau, Nassim Fares
Heart failure with preserved ejection fraction (HFpEF) is a common clinical syndrome associated with high morbidity and mortality. Therapeutic options are limited due to a lack of knowledge of the pathology and its evolution. We investigated the cellular phenotype and Ca2+ handling in hearts recapitulating HFpEF criteria. HFpEF was induced in a portion of male Wistar rats four weeks after abdominal aortic banding. These animals had nearly normal ejection fraction and presented elevated blood pressure, lung congestion, concentric hypertrophy, increased LV mass, wall stiffness, impaired active relaxation and passive filling of the left ventricle, enlarged left atrium, and cardiomyocyte hypertrophy...
January 2019: Biochimica et biophysica acta. Molecular basis of disease
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