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Stem Cell banking

Lijie Liu, Chuanying Xuan, Pei Shen, Tingting He, Ying Chang, Lijuan Shi, Shan Tao, Zhiping Yu, Richard E Brown, Jian Wang
Sensorineural hearing loss (SNHL) has been demonstrated in many clinical reports as a risk factor that promotes the development of cognitive impairment. However, the underlying neurological mechanisms are not clear. Noise exposure is one of the most common causes of SNHL. Although noise exposure causes relatively less damage to general health as compared with other methods for creating hearing loss (such as ototoxicity), it does impair cognitive function. Many studies have shown that the noise-induced cognitive impairment occur via the oxidative stress induced by the noise...
2018: Frontiers in Systems Neuroscience
Samantha Stewart, Xiaoming He
Advances in stem cell technology and regenerative medicine have underscored the need for effective baking of living cells. Cryopreservation, using very low temperatures to achieve suspended animation, is widely used to store or bank cells for later use. This process requires the use of cryoprotective agents (CPAs) to protect cells against damage caused by the freezing and thawing process. However, current popular CPAs like DMSO can be toxic to cells and must be thoroughly removed from cells before they can be used for research or clinical applications...
August 4, 2018: Langmuir: the ACS Journal of Surfaces and Colloids
Charles Osei-Bempong, Ali E Ghareeb, Majlinda Lako, Francisco C Figueiredo, W John Armitage
Limbal stem cell (LSC) deficiency causes progressive loss of vision but may be treated by transplant of autologous LSCs. Cryopreservation has the potential to indefinitely extend the lifespan of LSCs allowing re-transplant in case of graft failure. In this study, we aimed to identify the optimal cryoprotectant and cryoprotectant concentration for LSC cultures. Suspension cultures derived from cadaveric corneoscleral rims were cooled to 4 °C with Me2 SO, propylene glycol or ethylene glycol at a concentration of 5%, 10% or 15%...
July 31, 2018: Cryobiology
Ana Gomes Paz, Hassan Maghaireh, Francesco Guido Mangano
Stem cells are undifferentiated cells, capable of renewing themselves, with the capacity to produce different cell types to regenerate missing tissues and treat diseases. Oral facial tissues have been identified as a source and therapeutic target for stem cells with clinical interest in dentistry. This narrative review report targets on the several extraoral- and intraoral-derived stem cells that can be applied in dentistry. In addition, stem cell origins are suggested in what concerns their ability to differentiate as well as their particular distinguishing quality of convenience and immunomodulatory for regenerative dentistry...
2018: Stem Cells International
Marcie A Glicksman
Cell therapy has existed since the first bone marrow transplant in the 1950s involving identical twins. The blood-forming stem cells were used to restore healthy blood cells for the twin with leukemia. It was not until 1968 that genetic matching (known as human leukocyte antigen matching) was known to be important, and not until 1973 that bone marrow transplants were performed from non-twin-related and nonrelated donors. The most important application of human stem cells is for the generation of cells and tissues for cell-based therapies...
July 2018: Clinical Therapeutics
Peter Mazonson, Mark Kane, Kelin Colberg, Heather Harris, Heather Brown, Andrew Mohr, Chris Santas, Adrian Sandler
BACKGROUND: Although cord blood (CB) stem cell research is being conducted for treatment of cerebral palsy (CP), little is known about children with CP and stored CB. AIMS: To compare demographic and clinical characteristics of children with CP and stored CB to children with CP identified in a population-based study. METHODS AND PROCEDURES: The Longitudinal Umbilical Stem cell monitoring and Treatment REsearch (LUSTRE®) Registry recruited children from the largest US private CB bank...
September 2018: Research in Developmental Disabilities
Suji Lee, Ji Young Huh, David M Turner, Soohyeon Lee, James Robinson, Jeremy E Stein, Sung Han Shim, Chang Pyo Hong, Myung Seo Kang, Masato Nakagawa, Shin Kaneko, Mahito Nakanishi, Mahendra S Rao, Andreas Kurtz, Glyn N Stacey, Steven G E Marsh, Marc L Turner, Jihwan Song
Although autologous induced pluripotent stem cells (iPSCs) can potentially be useful for treating patients without immune rejection, in reality it will be extremely expensive and labor-intensive to make iPSCs to realize personalized medicine. An alternative approach is to make use of human leukocyte antigen (HLA) haplotype homozygous donors to provide HLA matched iPSC products to significant numbers of patients. To establish a haplobank of iPSCs, we repurposed the cord blood bank by screening ∼4,200 high resolution HLA typed cord blood samples, and selected those homozygous for the 10 most frequent HLA-A,-B,-DRB1 haplotypes in the Korean population...
July 13, 2018: Stem Cells
Jingyi Xiao, Dawei Yang, Qiwen Li, Weidong Tian, Weihua Guo
BACKGROUND: The concept of establishing a dental stem cell (DSC) bank for oral and maxillofacial regeneration has become of great interest but it remains at a primitive stage. The routine application of serum-containing conditions for human DSC (hDSC) culture is in great controversy considering that the animal-originated serum can cause serious ethical concerns and lead to increasingly irrelevant variables, errors, and poor repeatability of experiment results. Thus, this study aimed to establish a safe, stable and efficient hDSC serum-free culturing system for future DSC bank usage...
July 11, 2018: Stem Cell Research & Therapy
Revathiswari Tirughana, Marianne Z Metz, Zhongqi Li, Christine Hall, David Hsu, Jim Beltzer, Alexander J Annala, Diana Oganesyan, Margarita Gutova, Karen S Aboody
Cell-based therapies hold great promise for a myriad of clinical applications. However, as these therapies move from phase I to phase II and III trials, there is a need to improve scale-up of adherent cells for the production of larger good manufacturing practice (GMP) cell banks. As we advanced our neural stem cell (NSC)-mediated gene therapy trials for glioma to include dose escalation and multiple treatment cycles, GMP production using cell factories (CellStacks) generated insufficient neural stem cell (NSC) yields...
September 21, 2018: Molecular Therapy. Methods & Clinical Development
Hye Young Choi, So-Jung Kim, Gue Youn Go, Ara Kwon, Young Sam Im, Hye-Yeong Ha, Jin Tae Hong, Ji-Won Jung, Soo Kyung Koo
We generated a human induced pluripotent stem cell (hiPSC) line, KSCBi003-A, from adipose tissue-derived mesenchymal stem cells (Ad-MSCs) using a Sendai virus-based gene delivery system. We confirmed that the KSCBi003-A has a normal karyotype and short tandem repeat (STR)-based identities that match the parent cells. We also confirmed that the cell line expresses pluripotent stem cell markers such as Nanog, OCT4, SSEA-4, TRA-1-60, and TRA-1-81. We also analyzed that the KSCBi003-A has an ability to differentiate three germ layers (ectoderm, mesoderm, endoderm)...
June 27, 2018: Stem Cell Research
Anupam Sachdeva, Vinod Gunasekaran, Payal Malhotra, Dinesh Bhurani, Satya Prakash Yadav, Nita Radhakrishnan, Manas Kalra, Sunil Bhat, Ruchira Misra, Pramod Jog
JUSTIFICATION: Practitioners and people need information about the therapeutic potential of umbilical cord blood stem cells and pros and cons of storing cord blood in public versus private banks. PROCESS: Indian Academy of Pediatrics conducted a consultative meeting on umbilical cord blood banking on 25th June 2016 in Pune, attended by experts in the field of hematopoietic stem cell transplantation working across India. Review of scientific literature was also performed...
June 15, 2018: Indian Pediatrics
Yuki Ohta, Mitsuko Takenaga, Akemi Hamaguchi, Masanori Ootaki, Yuko Takeba, Tsukasa Kobayashi, Minoru Watanabe, Taroh Iiri, Naoki Matsumoto
Adipose tissue contains multipotent cells known as adipose-derived stem/stromal cells (ASCs), which have therapeutic potential for various diseases. Although the demand for adipose tissue for research use remains high, no adipose tissue bank exists. In this study, we attempted to isolate ASCs from cryopreserved adipose tissue with the aim of developing a banking system. ASCs were isolated from fresh and cryopreserved adipose tissue of rats and compared for proliferation (doubling time), differentiation capability (adipocytes), and cytokine (hepatocyte growth factor and vascular endothelial growth factor) secretion...
July 5, 2018: International Journal of Molecular Sciences
Sulmaz Kamalifar, Negar Azarpira, Ladan Sadeghi, Sadegh Ghorbani-Dalini, Seideh Masoomeh Nekoei, Mahdokht H Aghdaie, Elaheh Esfandiari, Mohamad Reza Azarpira
OBJECTIVES: Wharton jelly mesenchymal stem cells are good candidates for application in different aspects of regenerative medicine, and their long-time banking is important. In this study, the effects of trehalose, ascorbic acid, and Y-27632 on proliferation and survival rate of these cells after cryopreservation were investigated. MATERIALS AND METHODS: Mesenchymal stem cells were isolated from human umbilical cord Wharton jelly and frozen using a slow-rate cooling process...
June 28, 2018: Experimental and Clinical Transplantation
Shiva Mohamadi Moghanjoghi, Meysam Ganjibakhsh, Neda Sadat Gohari, Mehrnaz Izadpanah, Hedieh Rahmati, Zahra Elyasi Gorji, Nazanin Mohebali, Faezeh Vakhshiteh, Parvaneh Farzaneh
Some of lizard species have the ability to lose their tail in order to defend against predators and regenerate the new tail. Lizard's regenerated tail has attracted scientists' attention for unraveling the regeneration process, but less information is known about the cellular characterization and cell growth properties of original tail. This research aimed to report cell culture and banking process of rough-tailed gecko or Cyrtopodion scabrum's original tail cell sample from inner tissue without skin using tissue explant technique...
June 8, 2018: Cytotechnology
Valentina Arsić Arsenijević, David W Denning
For the first time, we aimed to estimate the burden of serious fungal infections or diseases (SFD) and highlight national epidemiological features in Serbia. Data on population and underlining conditions were extracted from the Statistical Office of the Republic of Serbia, World Bank, the Institute of Public Health of Serbia, the World Health Organization, National reference laboratory for medical mycology, the national registries of Serbian professional societies, and relevant publications. The population structure/inhabitants in 2016 (not including the autonomous region Kosovo & Metohija) was 7,058,322; with 6,041,743 adults (85...
June 25, 2018: Journal of Fungi (Basel, Switzerland)
Haihui Gu, Xia Huang, Jing Xu, Lili Song, Shuping Liu, Xiao-Bing Zhang, Weiping Yuan, Yanxin Li
BACKGROUND: Generation of induced pluripotent stem cells (iPSCs) from human peripheral blood provides a convenient and low-invasive way to obtain patient-specific iPSCs. The episomal vector is one of the best approaches for reprogramming somatic cells to pluripotent status because of its simplicity and affordability. However, the efficiency of episomal vector reprogramming of adult peripheral blood cells is relatively low compared with cord blood and bone marrow cells. METHODS: In the present study, integration-free human iPSCs derived from peripheral blood were established via episomal technology...
June 15, 2018: Stem Cell Research & Therapy
Michal Izrael, Shalom Guy Slutsky, Tamar Admoni, Louisa Cohen, Avital Granit, Arik Hasson, Joseph Itskovitz-Eldor, Lena Krush Paker, Graciela Kuperstein, Neta Lavon, Shiran Yehezkel Ionescu, Leonardo Javier Solmesky, Rachel Zaguri, Alina Zhuravlev, Ella Volman, Judith Chebath, Michel Revel
BACKGROUND: Amyotrophic lateral sclerosis (ALS) is a motor neuron (MN) disease characterized by the loss of MNs in the central nervous system. As MNs die, patients progressively lose their ability to control voluntary movements, become paralyzed and eventually die from respiratory/deglutition failure. Despite the selective MN death in ALS, there is growing evidence that malfunctional astrocytes play a crucial role in disease progression. Thus, transplantation of healthy astrocytes may compensate for the diseased astrocytes...
June 6, 2018: Stem Cell Research & Therapy
Mahendra S Rao, Ying Pei, Thelma Y Garcia, Shereen Chew, Toshiharu Kasai, Tomoko Hisai, Hideki Taniguchi, Takanori Takebe, Deepak A Lamba, Xianmin Zeng
We have previously reported the generation of a current Good Manufacture Practice (cGMP)-compliant induced pluripotent stem cell (iPSC) line for clinical applications. Here we show that multiple cellular products currently being considered for therapy can be generated from a single master cell bank of this or any other clinically compliant iPSC line METHODS: Using a stock at passage 20 prepared from the cGMP-compliant working cell bank (WCB), we tested differentiation into therapeutically relevant cell types of the three germ layers using standardized but generic protocols...
June 2018: Cytotherapy
Carla Dessels, Marco Alessandrini, Michael Sean Pepper
Hematopoietic stem cell transplantation (HSCT) is common practice today for life threatening malignant and non-malignant diseases of the blood and immune systems. Umbilical cord blood (UCB) is rich in hematopoietic stem cells (HSCs) and is an attractive alternative to harvesting HSCs from bone marrow or when mobilized into peripheral blood. One of the most appealing attributes of UCB is that it can be banked for future use and hence provides an off-the-shelf solution for patients in urgent need of a transplantation...
May 18, 2018: Stem Cells Translational Medicine
Dunia Jawdat, Sulaiman AlTwijri, Hadeel AlSemari, Mayssa Saade, Ahmed Alaskar
Background: In the last decade, cord blood (CB) has proven to be a valuable source of hematopoietic stem cells for transplantation to treat many hematological disorders. Since then, many CB banks have been established worldwide. Our aim was to estimate the level of public awareness of CB banking in Saudi Arabia. Study Design and Methods: A self-administered questionnaire of 22 multiple choices was conveniently distributed, consisting of demographics, awareness measure, attitude toward banking preference, and donation for research data...
2018: Stem Cells International
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