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D-Psicose

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https://www.readbyqxmd.com/read/30320068/adaptive-steered-molecular-dynamics-combined-with-protein-structure-networks-revealing-the-mechanism-of-y68i-g109p-mutations-that-enhance-the-catalytic-activity-of-d-psicose-3-epimerase-from-clostridium-bolteae
#1
Jingxuan Zhu, Yi Li, Jinzhi Wang, Zhengfei Yu, Ye Liu, Yi Tong, Weiwei Han
The scarcity, richness, and other important physiological functions of D-psicose make it crucial to increase the yield of D-psicose. The production of D-psicose can be accomplished by D-psicose 3-epimerase (DPEase) from Clostridium bolteae ( Cb DPEase) catalyzing the substrate D-fructose. Although the catalytic efficiency of the Cb DPEase has been raised via using the site-directed mutagenesis (Y68I/G109P) technique, structure-activity relationship in the wild-type Cb DPEase and Y68I/G109P mutant is currently poorly understood...
2018: Frontiers in Chemistry
https://www.readbyqxmd.com/read/30279320/x-ray-structure-of-arthrobacter-globiformis-m30-ketose-3-epimerase-for-the-production-of-d-allulose-from-d-fructose
#2
Hiromi Yoshida, Akihide Yoshihara, Pushpa Kiran Gullapalli, Kouhei Ohtani, Kazuya Akimitsu, Ken Izumori, Shigehiro Kamitori
The X-ray structure of ketose 3-epimerase from Arthrobacter globiformis M30, which was previously reported to be a D-allulose 3-epimerase (AgD-AE), was determined at 1.96 Å resolution. The crystal belonged to the hexagonal space group P65 22, with unit-cell parameters a = b = 103.98, c = 256.53 Å. The structure was solved by molecular replacement using the structure of Mesorhizobium loti L-ribulose 3-epimerase (MlL-RE), which has 41% sequence identity, as a search model. A hexagonal crystal contained two molecules in the asymmetric unit, and AgD-AE formed a homotetramer with twofold symmetry...
October 1, 2018: Acta Crystallographica. Section F, Structural Biology Communications
https://www.readbyqxmd.com/read/30255676/-research-progress-of-d-psicose-function-and-its-biosynthesis
#3
Xuemei Shen, Jing Wang, Yuan Zhang, Xiaoyan Wang, Ziyuan Ding, Yi Li, Bo Chen, Yi Tong
As the morbidity of metabolic syndrome like obesity and diabetes increases rapidly worldwide, the issue of nutrition (functional food) and health has drawn more attention. D-psicose, a rare natural ketohexose, has become a hot topic in functional food and health-care field because of its hypoglycemic and hypolipidemic function with good sweetness. This article mainly discusses the functional properties and biosynthesis research progress of D-psicose, together with the crystal structure of ketose-3-epimerase, to provide theoretical guidance for D-psicose-producing strain screening as well as improving the thermostability and catalytic efficiency of ketose-3-epimerase for industrial application...
September 25, 2018: Sheng Wu Gong Cheng Xue Bao, Chinese Journal of Biotechnology
https://www.readbyqxmd.com/read/30033987/-toxicity-of-d-arabinose-in-male-and-female-rats
#4
Shunsuke Higaki, Tatsuhiro Matsuo
In order to investigate the feasibility of using the rare sugar d-arabinose as a functional food material, we examined its toxicity in rats. In an acute toxicity study, the lethal dose (LD50 ) was calculated to be 12.1 g/kg in males and 11.6 g/kg in females. On the other hand, in a short-term toxicity test, rats developed diarrhea when given feed containing 5% d-arabinose, which was the minimum amount added, so the maximum nontoxic amount was estimated to be less than 5%. Thus, the toxicity of d-arabinose is stronger than that of another rare sugar, d-psicose, which was reported to show no toxicity when added at 10% in the diet...
2018: Shokuhin Eiseigaku Zasshi. Journal of the Food Hygienic Society of Japan
https://www.readbyqxmd.com/read/29687334/cell-regeneration-and-cyclic-catalysis-of-engineered-kluyveromyces-marxianus-of-a-d-psicose-3-epimerase-gene-from-agrobacterium-tumefaciens-for-d-allulose-production
#5
Peizhou Yang, Xingxing Zhu, Zhi Zheng, Dongdong Mu, Shaotong Jiang, Shuizhong Luo, Yun Wu, Minrui Du
D-Allulose as a low-energy and special bioactive monosaccharide sugar is essential for human health. In this study, the D-psicose-3-epimerase gene (DPEase) of Agrobacterium tumefaciens was transferred into thermotolerant Kluyveromyces marxianus to decrease the production cost of D-allulose and reduce the number of manufacturing procedures. The cell regeneration of K. marxianus and cyclic catalysis via whole-cell reaction were investigated to achieve the sustainable application of K. marxianus and the consumption of residual D-fructose...
April 23, 2018: World Journal of Microbiology & Biotechnology
https://www.readbyqxmd.com/read/29680868/an-integrated-bio-process-for-production-of-functional-biomolecules-utilizing-raw-and-by-products-from-dairy-and-sugarcane-industries
#6
Kusum Lata, Manisha Sharma, Satya Narayan Patel, Rajender S Sangwan, Sudhir P Singh
The study investigated an integrated bioprocessing of raw and by-products from sugarcane and dairy industries for production of non-digestible prebiotic and functional ingredients. The low-priced feedstock, whey, molasses, table sugar, jaggery, etc., were subjected to transglucosylation reactions catalyzed by dextransucrase from Leuconostoc mesenteroides MTCC 10508. HPLC analysis approximated production of about 11-14 g L-1 trisaccharide i.e. 2-α-D-glucopyranosyl-lactose (4-galactosyl-kojibiose) from the feedstock prepared from table sugar, jaggery, cane molasses and liquid whey, containing about 30 g L-1 sucrose and lactose each...
August 2018: Bioprocess and Biosystems Engineering
https://www.readbyqxmd.com/read/29402406/secretion-of-glp-1-but-not-gip-is-potently-stimulated-by-luminal-d-allulose-d-psicose-in-rats
#7
Masaki Hayakawa, Tohru Hira, Masako Nakamura, Tetsuo Iida, Yuka Kishimoto, Hiroshi Hara
Glucagon-like peptide 1 (GLP-1), an incretin gastrointestinal hormone, is secreted when stimulated by nutrients including metabolizable sugars such as glucose and fructose. d-Allulose (allulose), also known as d-psicose, is a C-3 isomer of d-fructose and a rare sugar with anti-diabetic or anti-obese effects in animal models. In the present study, we examined whether an oral administration of allulose could stimulate GLP-1 secretion in rats, and investigated the underlying mechanisms. Oral, but not intraperitoneal, administration of allulose (0...
February 2, 2018: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/29317623/glp-1-release-and-vagal-afferent-activation-mediate-the-beneficial-metabolic-and-chronotherapeutic-effects-of-d-allulose
#8
Yusaku Iwasaki, Mio Sendo, Katsuya Dezaki, Tohru Hira, Takehiro Sato, Masanori Nakata, Chayon Goswami, Ryohei Aoki, Takeshi Arai, Parmila Kumari, Masaki Hayakawa, Chiaki Masuda, Takashi Okada, Hiroshi Hara, Daniel J Drucker, Yuichiro Yamada, Masaaki Tokuda, Toshihiko Yada
Overeating and arrhythmic feeding promote obesity and diabetes. Glucagon-like peptide-1 receptor (GLP-1R) agonists are effective anti-obesity drugs but their use is limited by side effects. Here we show that oral administration of the non-calorie sweetener, rare sugar D-allulose (D-psicose), induces GLP-1 release, activates vagal afferent signaling, reduces food intake and promotes glucose tolerance in healthy and obese-diabetic animal models. Subchronic D-allulose administered at the light period (LP) onset ameliorates LP-specific hyperphagia, visceral obesity, and glucose intolerance...
January 9, 2018: Nature Communications
https://www.readbyqxmd.com/read/29132732/the-role-of-artificial-and-natural-sweeteners-in-reducing-the-consumption-of-table-sugar-a-narrative-review
#9
REVIEW
Arshag D Mooradian, Meridith Smith, Masaaki Tokuda
The rapid increase in the prevalence of obesity worldwide has been partially attributed to the overconsumption of added sugars. Recent guidelines call for limiting the consumption of simple sugars to less than 10% of daily caloric consumption. High intensity sweeteners are regulated as food additives and include aspartame, acesulfame-k, neotame, saccharin, sucralose, cyclamate and alitame. Steviol glycosides and Luo Han Guo fruit extracts are high intensity sweeteners that are designated as generally recognized as safe (GRAS)...
April 2017: Clinical Nutrition ESPEN
https://www.readbyqxmd.com/read/29124517/characterization-of-l-rhamnose-isomerase-from-clostridium-stercorarium-and-its-application-to-the-production-of-d-allose-from-d-allulose-d-psicose
#10
Min-Ju Seo, Ji-Hyeon Choi, Su-Hwan Kang, Kyung-Chul Shin, Deok-Kun Oh
OBJECTIVE: To characterize L-rhamnose isomerase (L-RI) from the thermophilic bacterium Clostridium stercorarium and apply it to produce D-allose from D-allulose. RESULTS: A recombinant L-RI from C. stercorarium exhibited the highest specific activity and catalytic efficiency (k cat /K m ) for L-rhamnose among the reported L-RIs. The L-RI was applied to the high-level production of D-allose from D-allulose. The isomerization activity for D-allulose was maximal at pH 7, 75 °C, and 1 mM Mn2+ over 10 min reaction time...
February 2018: Biotechnology Letters
https://www.readbyqxmd.com/read/29121933/biochemical-analysis-and-the-preliminary-crystallographic-characterization-of-d-tagatose-3-epimerase-from-rhodobacter-sphaeroides
#11
Zhengliang Qi, Zhangliang Zhu, Jian-Wen Wang, Songtao Li, Qianqian Guo, Panpan Xu, Fuping Lu, Hui-Min Qin
BACKGROUND: D-Tagatose 3-epimerase epimerizes D-fructose to yield D-psicose, which is a rare sugar that exists in small quantities in nature and is difficult to synthesize chemically. We aim to explore potential industrial biocatalysts for commercial-scale manufacture of this rare sugar. A D-tagatose 3-epimerase from Rhodobacter sphaeroides (RsDTE) has recently been identified as a D-tagatose 3-epimerase that can epimerize D-fructose to yield D-psicose with a high conversion rate. RESULTS: The purified RsDTE by Ni-affinity chromatography, ionic exchange chromatography and gel filtration forms a tetramer in solution...
November 9, 2017: Microbial Cell Factories
https://www.readbyqxmd.com/read/29038926/deactivation-kinetics-and-the-effects-of-additives-on-storage-stability-and-structure-of-d-psicose-3-epimerase
#12
Qiao Zhang, Bo Jiang, Tao Zhang, Zhenhua Duan
OBJECTIVE: To explore deactivation kinetics and the effects of some additives on the activity and conformational changes of D-psicose 3-epimerase (DPEase) during its storage. RESULTS: The experimental data of DPEase inactivation during storage at 4-45 °C fitted with the first-order expression model. The inactivation rate constants of DPEase stored at 4, 10, 25, 35 and 45 °C were 0.0076, 0.01, 0.0223, 0.0351 and 0.0605 day, respectively. A regression formula of half-lives as storage temperatures, ln t 1/2  = 4...
January 2018: Biotechnology Letters
https://www.readbyqxmd.com/read/28988049/development-of-a-thermo-stable-and-recyclable-magnetic-nanobiocatalyst-for-bioprocessing-of-fruit-processing-residues-and-d-allulose-synthesis
#13
Satya Narayan Patel, Vishal Singh, Manisha Sharma, Rajender S Sangwan, Nitin K Singhal, Sudhir P Singh
The aim of the study was to covalently immobilize Smt3-D-psicose 3-epimerase onto functionalized iron oxide magnetic nanoparticles. After immobilization, Km of the immobilized enzyme increased, however, Vmax was nearly the same as that of its free form, indicating that immobilization has no detrimental effects on its catalytic output. The covalent immobilization caused a reduction in the deactivation rate constant (kd ) values leading to 4-5 fold enhancement in its half-life at 50-65°C, indicating significant thermal stability of the iron-enzyme nanobioconjugate...
January 2018: Bioresource Technology
https://www.readbyqxmd.com/read/28899486/immobilization-on-graphene-oxide-improves-the-thermal-stability-and-bioconversion-efficiency-of-d-psicose-3-epimerase-for-rare-sugar-production
#14
Samir R Dedania, Manisha J Patel, Dijit M Patel, Rekha C Akhani, Darshan H Patel
D-Psicose (D-ribo-2-hexulose or D-allulose), an epimer of D-fructose is considered as a rare low-calorie sugar displaying important physiological functions. Enzymatic production using ketose 3-epimerases is the feasible process for the production of D-Psicose. However, major drawbacks in application of ketose 3-epimerases are bioconversion efficiency and reusability of the enzyme. We have attempted immobilization of ketose 3-epimerases from Agrobacterium tumefaciens (agtu) D-psicose 3-epimerase (DPEase) on graphene oxide...
December 2017: Enzyme and Microbial Technology
https://www.readbyqxmd.com/read/28851162/simultaneous-production-of-bioethanol-and-value-added-d-psicose-from-jerusalem-artichoke-helianthus-tuberosus-l-tubers
#15
Younho Song, Chihoon Oh, Hyeun-Jong Bae
In this study, the production of bioethanol and value added d-psicose from Jerusalem artichoke (JA) was attempted by an enzymatic method. An enzyme mixture used for hydrolysis of 100mgmL(-1) JA. The resulting concentrations of released d-fructose and d-glucose were measured at approximately 56mgmL(-1) and 15mgmL(-1), respectively. The d-psicose was epimerized from the JA hydrolyzate, and the conversion rate was calculated to be 32.1%. The residual fructose was further converted into ethanol at 18.0gL(-1) and the yield was approximately 72%...
November 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28757536/comparison-of-anti-obesity-effect-between-two-types-of-syrup-containing-rare-sugars-in-wistar-rats
#16
COMPARATIVE STUDY
Masaru Ochiai, Kohei Misaki, Takako Yamada, Tetsuo Iida, Kazuhiro Okuma, Tatsuhiro Matsuo
D-Allulose-containing rare sugar sweeteners have been categorized into two types, rare sugar syrup (RSS), consisting of 4 rare monosaccharides, and modified glucose syrup (MGS), rich in D-allulose, which was previously referred to D-psicose. The anti-obesity effect of RSS and D-allulose has been already clarified, but that of rare monosaccharides other than D-allulose in RSS has not yet been well understood. Here, we investigated and compared the anti-obesity effect of RSS and MGS in rats. Male Wistar rats were divided into 4 dietary groups: a high-sucrose control diet group (S), a high-fructose corn syrup diet group (HFCS), an RSS diet group (RSS), and an MGS diet group (MGS)...
2017: Journal of Nutritional Science and Vitaminology
https://www.readbyqxmd.com/read/28756856/production-of-d-psicose-from-d-glucose-by-co-expression-of-d-psicose-3-epimerase-and-xylose-isomerase
#17
Xiaoyan Chen, Wen Wang, Jingliang Xu, Zhenhong Yuan, Tao Yuan, Yu Zhang, Cuiyi Liang, Minchao He, Ying Guo
d-Psicose has been drawing increasing attention in recent years because of its medical and health applications. The production of d-psicose from d-glucose requires the co-expression and synergistic action of xylose isomerase and d-psicose 3-epimerase. To co-express these genes, vector pET-28a(+)-dual containing two T7 promoters and RBS sites and an Multiple Cloning Sites was constructed using the Escherichia coli expression plasmid pET-28a(+). The xylose isomerase gene from E. coli MG1665 and the d-psicose 3-epimerase gene from Agrobacterium tumefaciens CGMCC 1...
October 2017: Enzyme and Microbial Technology
https://www.readbyqxmd.com/read/28276482/multimer-recognition-and-secretion-by-the-non-classical-secretion-pathway-in-bacillus-subtilis
#18
Liuqun Zhao, Jingqi Chen, Jibin Sun, Dawei Zhang
Non-classical protein secretion in bacteria is a common phenomenon. However, the selection principle for non-classical secretion pathways remains unclear. Here, our experimental data, to our knowledge, are the first to show that folded multimeric proteins can be recognized and excreted by a non-classical secretion pathway in Bacillus subtilis. We explored the secretion pattern of a typical cytoplasmic protein D-psicose 3-epimerase from Ruminococcus sp. 5_1_39BFAA (RDPE), and showed that its non-classical secretion is not simply due to cell lysis...
March 9, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28191448/a-food-grade-expression-system-for-d-psicose-3-epimerase-production-in-bacillus-subtilis-using-an-alanine-racemase-encoding-selection-marker
#19
Jingqi Chen, Zhaoxia Jin, Yuanming Gai, Jibin Sun, Dawei Zhang
BACKGROUND: Food-grade expression systems require that the resultant strains should only contain materials from food-safe microorganisms, and no antibiotic resistance marker can be utilized. To develop a food-grade expression system for d-psicose 3-epimerase production, we use an alanine racemase-encoding gene as selection marker in Bacillus subtilis. RESULTS: In this study, the d-alanine racemase-encoding gene dal was deleted from the chromosome of B. subtilis 1A751 using Cre/lox system to generate the food-grade host...
2017: Bioresources and Bioprocessing
https://www.readbyqxmd.com/read/28190218/drude-polarizable-force-field-for-aliphatic-ketones-and-aldehydes-and-their-associated-acyclic-carbohydrates
#20
Meagan C Small, Asaminew H Aytenfisu, Fang-Yu Lin, Xibing He, Alexander D MacKerell
The majority of computer simulations exploring biomolecular function employ Class I additive force fields (FF), which do not treat polarization explicitly. Accordingly, much effort has been made into developing models that go beyond the additive approximation. Development and optimization of the Drude polarizable FF has yielded parameters for selected lipids, proteins, DNA and a limited number of carbohydrates. The work presented here details parametrization of aliphatic aldehydes and ketones (viz. acetaldehyde, propionaldehyde, butaryaldehyde, isobutaryaldehyde, acetone, and butanone) as well as their associated acyclic sugars (D-allose and D-psicose)...
April 2017: Journal of Computer-aided Molecular Design
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