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solid state fermentation

Amira M Embaby, Mohamed N Hussein, Ahmed Hussein
The present study underlines a statistically optimized, low cost, effective approach for efficient co-valorization of two non-efficiently utilized, highly accumulated, raw agro-industrial wastes: corn cob and glycerol for co-production of natural biopigments: monascus orange and red pigments by the aid of Monascus purpureus strain ATCC 16436. A three step sequential, statistical modeling approach: one variable at a time (OVAT), Plackett-Burman design (PBD), and central composite design (CCD) was employed to optimize the production of monascus pigments using co-solid state fermentation of the two raw agro-industrial wastes...
2018: PloS One
Paul W Baker, Adam Charlton, Mike D C Hale
The application of solid state fermentation offers an alternative to conventional, submerged approaches for a variety of bioconversion processes, including animal feeds, biofuels and fungal bioproducts. Optimising solid state fermentation under low moisture conditions could significantly impact the proportion of dry biomass that could be processed and improve the commercial viability of this approach, because of reduced input costs and higher yields of final products. Pleurotus erygnii that appeared to show tolerance to low moisture conditions was grown on saturated and desaturated wheat straw...
December 5, 2018: Letters in Applied Microbiology
Vladimir Elisashvili, Eva Kachlishvili, Michael L Chikindas
Spore-forming probiotic bacteria have received a wide and constantly increasing scientific and commercial interest. Among them, Bacillus species are the most studied and well-characterized Gram-positive bacteria. The use of bacilli as probiotic products is expanding especially rapidly due to their inherent ability to form endospores with unique survivability and tolerance to extreme environments and to produce a large number of valuable metabolites coupled with their bio-therapeutic potential demonstrating immune stimulation, antimicrobial activities and competitive exclusion...
December 5, 2018: Probiotics and Antimicrobial Proteins
Lei Feng, Yufeng Xie, Chenmiao Peng, Yuxiaoxue Liu, Haikuan Wang
Diabetes is a chronic metabolic disease characterized by hyperglycaemia and a number of potential complications that significantly reduce the patient's quality of life. In this study, we produced an antidiabetic functional food from Tartary buckwheat fermented by Lactobacillus plantarum TK9 and L. paracasei TK1501. The results of an orthogonal experimental design indicated that the three factors with the largest effects on the growth of L. plantarum TK9 and L. paracasei TK1501 in solid-state fermentation (SSF) were in the order: water ratio>inoculum size>time of fermentation...
September 2018: Food Technology and Biotechnology
Jelena Jović, Aneta Buntić, Neda Radovanović, Bojan Petrović, Ljiljana Mojović
The aim of this research is to isolate and identify fungi with high lignin-degrading abilities that are autochthonous to southern Serbian region. Two novel fungal isolates identified as Trametes hirsuta F13 and Stereum gausapatum F28 were selected to assess their ligninolytic enzyme activities and the efficiency of lignin removal from beech wood sawdust. Obtained results show that both isolates are good sources of industrially valuable enzymes with a potential for application in various biotechnological and industrial processes...
September 2018: Food Technology and Biotechnology
Ruolin Hou, Xin Liu, Kaikai Xiang, Liangtao Chen, Xiaoping Wu, Wenxiong Lin, Mingfeng Zheng, Junsheng Fu
In this study, solid-state fermentation was used to produce Inonotus hispidus melanin (IH melanin). Its physicochemical properties were characterized, and ultrasonic-assisted extraction was used to optimize its extraction process. Characterization techniques like elemental analysis and so on were used to identify melanin. The solubility, stability, and antioxidant activity were also measured. Furthermore, the extraction process of IH melanin was optimized. The results showed that the pigment could be defined as dihydroxy phenylalanine (DOPA)-melanin, it displayed irregular spherical and ellipsoidal structures with average size of 89...
March 30, 2019: Food Chemistry
Kumari Vibha, Sangeeta Negi
The present study deals with the coproduction of laccase and pectinase enzymes through solid state fermentation using mixed fungal culture of Trametes hirsuta and Phanerochaete sp., to minimize the cost and time of the process. Substrates selected for the enzyme production were wheat bran, pulse husk and mustard peel. To get optimum yield of laccase and pectinase in a single fermenter, EVOP factorial design technique with factors like pH, incubation temperature and substrates ratio have been explored. At search level of EVOP, outcomes of the one factor at a time has been considered as incubation temperature 30 °C for 7 days at pH 5 with wheat bran: pulse husk: mustard peel ratio of 2:2:1 (w/w/w) and yield of laccase and pectinase was found as 78...
December 2018: 3 Biotech
Wenmeng He, Hau Yin Chung
BACKGROUND: Red sufu is a type of sufu produced by solid-state fermentation of soybean curd and coloration with red mold rice. The purposes of this study were 1) to characterize commercial red sufu samples using the Quantitative Descriptive Analysis (QDA) and Flash Profile (FP) by ten trained and ten untrained panelists, respectively; 2) to compare the differences in panel performance, descriptive abilities, and sensory maps between the two methodologies; and 3) to compare the efficiency between QDA and FP using red sufu as the matrix...
November 28, 2018: Journal of the Science of Food and Agriculture
Jiancheng Liu, Hong Sun, Cunxi Nie, Wenxia Ge, Yongqiang Wang, Wenju Zhang
In this study, the immunomodulatory activity of oligopeptide (CP) derived from solid-state fermented cottonseed meal were investigated in immunosuppressed BALB/c mice models by treatment with cyclophosphamide (CY). Results indicated that oligopeptide increased the thymus and spleen indices of CY-treated mice. The count of plague forming cells (PFC) and the content of half serum hemolysis (HC50 ) in immunosuppressive mice were restored to the normal level in CP-10 and CP-20 groups while the cytokines interleukin (IL)-2, IL-6, and tumor necrosis factor alpha (TNF-α) were increased significantly in CP-20 group...
December 2018: Food Science and Biotechnology
Cheng Wang, Weifa Su, Yu Zhang, Lihong Hao, Fengqin Wang, Zeqing Lu, Jian Zhao, Xuelian Liu, Yizhen Wang
Solid-state fermentation (SSF) was carried out in this study to improve the nutritional digestibility of two types of distilled dried grain with solubles (DDGS) by inoculating probiotic combinations. The fermented DDGS (FDDGS) contained more crude protein, small peptides and total amino acids than did unfermented DDGS. The concentrations of fiber indexes significantly declined after fermentation. The amounts of probiotics, enzymes and organic acids were significantly improved after fermentation. Microscopy revealed that SSF disrupted the surface structure and increased small fragments of DDGS substrate, thereby facilitating in vitro digestibility of FDDGS...
November 26, 2018: AMB Express
Abha Sharma, Kavish Kumar Jain, Anita Srivastava, Bhuvnesh Shrivastava, Vasanta Vadde Thakur, R K Jain, R C Kuhad
Production of laccase from Ganoderma lucidum RCK 2011 under solid-state fermentation (SSF) conditions was optimized using response surface methodology, resulting in an approximate eightfold increase compared to that in the unoptimized media. Further, the enzyme produced under SSF as whole fermented substrate (in situ SSF laccase) was found to be more stable than the in vitro enzyme (harvested by downstreaming processing of fermented wheat bran). Interestingly, the biobleaching potentials of both in situ and in vitro SSF laccases were comparable, saving 25% chlorine dioxide for achieving similar pulp brightness as obtained in the pulp treated chemically...
November 23, 2018: Bioprocess and Biosystems Engineering
Dan-Dan Yin, Mo Yang, Yun-Lai Wang, Hao-Kun Liu, Min Zhou, Fan Xu
Ligustrazine is an important active ingredient of the traditional Chinese medicine Chuanxiong Rhizoma, but its content is a controversial topic. The endophytes of medicinal plants have the ability to produce the same active substances as the host, so this report focused on the endophytic Bacillus subtilis, to study the origin of ligustrazine in Chuanxiong Rhizoma preliminarily by inoculating the isolated endophytic B. subtilis to the Chuanxiong Rhizoma medium in vitro for solid state fermentation. Tissue grinding method was used to isolate the endogenetic B...
October 2018: Zhongguo Zhong Yao za Zhi, Zhongguo Zhongyao Zazhi, China Journal of Chinese Materia Medica
Xin Zeng, Wenyun Miao, Huawei Zeng, Kuo Zhao, Yaling Zhou, Juan Zhang, Qinxue Zhao, Delinur Tursun, Dayong Xu, Feng Li
At present, submerged fermentation (SmF) is the unique approach for natamycin production. This study aims to propose a strategy for natamycin production through solid-state fermentation (SSF). The maximum natamycin concentration (9.62 mg·gds-1 ) was obtained with a substrate mixture containing wheat bran, rapeseed cake, rice hull and crude glycerol in a 5 L flask at 28 °C, and the initial moisture content and inoculum size was set as 70% and 15%, individually. A 30 L scale-up fermentation showed similar parameters and produced 9...
November 5, 2018: Bioresource Technology
Li-Wen Su, Yeong-Hsiang Cheng, Felix Shih-Hsiang Hsiao, Jin-Cheng Han, Yu-Hsiang Yu
Soybean meal is the main vegetable protein source in animal feed. Soybean meal contains several anti-nutritional factors, which directly affect digestion and absorption of soy protein, thereby reducing growth performance and value in animals. Fermented soybean meal is rich in probiotics and functional metabolites, which facilitates soybean protein digestion, absorption and utilization in piglets. However, the mixed solid-state fermentation (SSF) conditions of soybean meal remain to be optimized. In this study, we investigated the optimal parameters for SSF of soybean meal by Lactobacillus species and Clostridium butyricum ...
2018: Polish Journal of Microbiology
Xin Liu, Ruolin Hou, Kaiqiang Xu, Lei Chen, Xiaoping Wu, Wenxiong Lin, Mingfeng Zheng, Junsheng Fu
A polysaccharide from Inonotus hispidus was prepared by solid-state fermentation (SSF), and an efficient ultrasound-assisted extraction method was used to optimize the extraction process of Inonotus hispidus solid-state fermentation polysaccharide (IHSFP). The optimal extraction parameters were as follows: solid-liquid ratio of 1:40, ultrasound time of 34 min, ultrasound power of 350 W and ultrasound temperature of 70 °C. The yield of IHSFP was 51.06 ± 0.34% under the optimal conditions. Two types of polysaccharide fractions (IHSFP-1 and IHSFP-2) were isolated by DEAE-52 and Sephadex G-200 columns...
November 13, 2018: International Journal of Biological Macromolecules
Deepankar Sharma, Kushagri Singh, Kavita Singh, Abha Mishra
L-asparaginase is a valuable protein therapeutic drug utilized for the treatment of leukemia and lymphomas. Administration of asparaginase leads to asparagine starvation causing inhibition of protein synthesis, growth, and proliferation of tumor cells. Besides its clinical significance, the enzyme also finds application in the food sector for mitigation of a cancer-causing agent acrylamide. The numerous applications ensue huge market demands and create a continued interest in the production of cost-effective, more specific, less immunogenic and stable formulations which can cater both the clinical and food processing requirements...
November 13, 2018: Current Protein & Peptide Science
Chaoya Zhao, Ting Xia, Peng Du, Wenhui Duan, Bo Zhang, Jin Zhang, Shenghu Zhu, Yu Zheng, Min Wang, Yongjian Yu
Zhenjiang aromatic vinegar (ZAV) is one of the well-known fermented condiments in China, which is produced by solid-state fermentation. It can be classified into traditional Zhenjiang aromatic vinegar (TZAV) and industrial Zhenjiang aromatic vinegar (IZAV) because of different production methods. The purpose of the study was to evaluate the variations and differences on chemical compositions and antioxidant activities of TZAV and IZAV during the aging process. The proximate composition, organic acids content, total phenolic content (TPC), total flavonoid content (TFC), total antioxidant activity (TAA) and phenolic compounds composition of TZAV and IZAV were detected during the aging process...
November 12, 2018: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
Yong Jiang, Harold D May, Lu Lu, Peng Liang, Xia Huang, Zhiyong Jason Ren
Carbon-rich waste materials (solid, liquid, or gaseous) are largely considered to be a burden on society due to the large capital and energy costs for their treatment and disposal. However, solid and liquid organic wastes have inherent energy and value, and similar as waste CO2 gas they can be reused to produce value-added chemicals and materials. There has been a paradigm shift towards developing a closed loop, biorefinery approach for the valorization of these wastes into value-added products, and such an approach enables a more carbon-efficient and circular economy...
November 2, 2018: Water Research
Antonella Marone, Eric Trably, Hélène Carrère, Pacôme Prompsy, Fabienne Guillon, Maud Joseph-Aimé, Abdellatif Barakat, Nour Fayoud, Nicolas Bernet, Renaud Escudié
Solid-state fermentation is a potential technology for developing lignocellulosic biomass-based biorefineries. This work dealt with solid-state fermentation for carboxylates production from corn stover, as building blocks for a lignocellulosic feedstock-based biorefinery. The effect of extrusion pretreatment, together with the action of a microbial consortia and hydrolytic enzymes as biotic triggers, was investigated on corn stover conversion, microbial metabolic pathways, and populations. The extrusion caused changes in the physical and morphological characteristics, without altering the biochemical composition of the corn stover...
November 7, 2018: Applied Microbiology and Biotechnology
Chunlei Yang, Zhongfa Chen, Yuelei Wu, Jiakun Wang
Objective: The experiment was conducted to evaluate the effects of four fungal pretreatments on the nutritional value of Camellia seed residues, and to evaluate the feeding value of pre-treated Camellia seed residues for ruminants. Methods: Camellia seed residues were firstly fermented by four lignin degrading fungi, namely, Phanerochaete chrysosporium-30942, Trichoderma koningiopsis-2660, Trichoderma aspellum-2527, or T. aspellum-2627, under solid-state fermentation (SSF) conditions at six different incubation times...
October 26, 2018: Asian-Australasian Journal of Animal Sciences
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