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Keywords Excisional Wound Healing In Th...

Excisional Wound Healing In The Mouse

https://read.qxmd.com/read/36978136/extracellular-vesicles-from-lactobacillus-druckerii-inhibit-hypertrophic-scar-fibrosis
#21
JOURNAL ARTICLE
Fu Han, Kejia Wang, Kuo Shen, Jing Wang, Shichao Han, Dahai Hu, Gaofeng Wu
BACKGROUND: Hypertrophic scars (HS) affect millions of people each year and require better treatment strategies. Bacterial extracellular vesicles (EVs) are advantaged by low cost and high yield which was commonly used in the treatment of diseases. Here, we investigated the therapeutic efficacy of EVs obtained from Lactobacillus druckerii in hypertrophic scar. In vitro, the effects of Lactobacillus druckerii-derived EVs (LDEVs) on Collagen I/III and α-SMA in fibroblasts obtained from HS...
March 28, 2023: Journal of Nanobiotechnology
https://read.qxmd.com/read/36974384/wound-healing-activity-of-arum-maculatum
#22
JOURNAL ARTICLE
Ayse Arzu Sakul, Mehmet Evren Okur, Sule Ayla, Benay Daylan, Ayse Esra Karadag, Sebnem Batur, Ekrem Musa Ozdemir, Ebrar Altinalan, Mehmet Yalcin Gunal
OBJECTIVE: In this study, the antioxidant properties of Arum maculatum plant were evaluated. This study reported for the first time the wound healing activity of the methanol extract of A. maculatum fruits. This study aimed to assess and determine the possible pharmacological activities of A. maculatum and evaluate its potential to act as a wound care plant. METHODS: The antioxidant and antimicrobial activities of A. maculatum were investigated using excisional in vivo and in vitro wound healing mouse models...
March 28, 2023: Medeniyet medical journal
https://read.qxmd.com/read/36902475/effect-of-chitosan-diosgenin-combination-on-wound-healing
#23
JOURNAL ARTICLE
Lubomir Petrov, Olya Stoilova, Georgi Pramatarov, Hristiyana Kanzova, Elina Tsvetanova, Madlena Andreeva, Almira Georgieva, Dimitrinka Atanasova, Stanislav Philipov, Albena Alexandrova
The difficult-to-heal wounds continue to be a problem for modern medicine. Chitosan and diosgenin possess anti-inflammatory and antioxidant effects making them relevant substances for wound treatment. That is why this work aimed to study the effect of the combined application of chitosan and diosgenin on a mouse skin wound model. For the purpose, wounds (6 mm diameter) were made on mice's backs and were treated for 9 days with one of the following: 50% ethanol (control), polyethylene glycol (PEG) in 50% ethanol, chitosan and PEG in 50% ethanol (Chs), diosgenin and PEG in 50% ethanol (Dg) and chitosan, diosgenin and PEG in 50% ethanol (ChsDg)...
March 6, 2023: International Journal of Molecular Sciences
https://read.qxmd.com/read/36897423/a-fabricated-hydrogel-of-hyaluronic-acid-curcumin-shows-super-activity-to-heal-the-bacterial-infected-wound
#24
JOURNAL ARTICLE
Maryam Khaleghi, Fakhri Haghi, Mina Gholami, Hamdam Hourfar, Farshad Shahi, Ali Mir Mousavi Zekoloujeh, Farhang Aliakbari, Ebrahim Ahmadi, Dina Morshedi
High risk of acute morbidities and even mortality from expanding the antibiotics resistant infectious wounds force indefinite efforts for development of high performance wound-healing materials. Herein, we design a procedure to fabricate a hyaluronic acid (HA)-based hydrogel to conjugate curcumin (Gel-H.P.Cur). The highlight of this work is to provide a favorite condition for capturing curcumin while protecting its structure and intensifying its activities because of the synchronization with HA. Accordingly, HA as a major component of dermis with a critical role in establishing skin health, could fortify the wound healing property as well as antibacterial activity of the hydrogel...
March 10, 2023: AMB Express
https://read.qxmd.com/read/36893465/development-of-neuropeptide-hemokinin-1-analogues-with-antimicrobial-and-wound-healing-activity
#25
JOURNAL ARTICLE
Yufan Yao, Wei Zhang, Sisi Li, Huan Xie, Zhengzheng Zhang, Bo Jia, Sujie Huang, Wenyuan Li, Ling Ma, Yuxuan Gao, Jingjing Song, Rui Wang
Wound healing is a complex process that can be delayed in some pathological conditions, such as infection and diabetes. Following skin injury, the neuropeptide substance P (SP) is released from peripheral neurons to promote wound healing by multiple mechanisms. Human hemokinin-1 (hHK-1) has been identified as an SP-like tachykinin peptide. Surprisingly, hHK-1 shares similar structural features with antimicrobial peptides (AMPs), but it does not display efficient antimicrobial activity. Therefore, a series of hHK-1 analogues were designed and synthesized...
March 9, 2023: Journal of Medicinal Chemistry
https://read.qxmd.com/read/36872305/splint-free-line-drawing-model-an-innovative-method-for-excisional-wound-models
#26
JOURNAL ARTICLE
Yi Yang, Wenjun Xie, Shiyi Li, Xuer Sun, Boya Yu, Huijuan Fu, Minliang Chen
The physiological phenomenon of wound contraction in mice cannot completely imitate the process of human skin regeneration, which is primarily attributed to reepithelialisation. As such, excisional wound models in mice are considered to be imperfect comparisons. This study aimed to enhance the correlation of mouse excisional wound models with that of humans, and to offer more practical and accurate ways to record and measure wound areas. We present evidence that simple excisional wounds produce a robust and stable wound model by comparing splint-free and splint groups...
March 5, 2023: International Wound Journal
https://read.qxmd.com/read/36614291/a%C3%A3-ai-berry-administration-promotes-wound-healing-through-wnt-%C3%AE-catenin-pathway
#27
JOURNAL ARTICLE
Livia Interdonato, Ylenia Marino, Gianluca Antonio Franco, Alessia Arangia, Ramona D'Amico, Rosalba Siracusa, Marika Cordaro, Daniela Impellizzeri, Roberta Fusco, Salvatore Cuzzocrea, Rosanna Di Paola
Recently, wound healing has received increased attention from both a scientific and clinical point of view. It is characterized by an organized series of processes: angiogenesis, cell migration and proliferation, extracellular matrix production, and remodeling. Many of these processes are controlled by the Wnt pathway, which activates them. The aim of the study was to evaluate the molecular mechanism of açai berry administration in a mouse model of wound healing. CD1 male mice were used in this research...
January 3, 2023: International Journal of Molecular Sciences
https://read.qxmd.com/read/36484112/topical-vanadate-improves-tensile-strength-and-alters-collagen-organization-of-excisional-wounds-in-a-mouse-model
#28
JOURNAL ARTICLE
Hendrik Lintel, Darren B Abbas, Duncan J Mackay, Michelle Griffin, Christopher V Lavin, Charlotte E Berry, Nicholas J Guardino, Jason L Guo, Arash Momeni, Donald R Mackay, Michael T Longaker, Derrick C Wan
Wound dehiscence, oftentimes a result of the poor tensile strength of early healing wounds, is a significant threat to the postoperative patient, potentially causing life-threatening complications. Vanadate, a protein tyrosine phosphatase inhibitor, has been shown to alter the organization of deposited collagen in healing wounds and significantly improve the tensile strength of incisional wounds in rats. In this study, we sought to explore the effects of locally administered vanadate on tensile strength and collagen organization in both the early and remodeling phases of excisional wound healing in a murine model...
December 9, 2022: Wound Repair and Regeneration
https://read.qxmd.com/read/36436910/impaired-healing-of-cutaneous-wound-in-a-trpv1-deficient-mouse
#29
JOURNAL ARTICLE
Kazuki Ueno, Shizuya Saika, Yuka Okada, Hiroki Iwanishi, Kentaro Suzuki, Gen Yamada, Shinichi Asamura
Transient Receptor Potential (TRP) ion channels mediate the influx of cations into cells responding to chemical or physical stimuli. TRP vanilloid 1 (TRPV1) regulates cutaneous functions. Its function in cutaneous wound healing, however, has not been clarified. The current study elucidated the role of TRPV1 in cutaneous wound healing of dorsal circular excisional injury using Trpv1-null (KO) and wild type (WT) male/female C57BL/6 mice. Macroscopic observation showed that the remaining cutaneous lesion was significantly larger in KO than that of WT at postoperative days (POD) 7 and 10...
November 25, 2022: Experimental Animals
https://read.qxmd.com/read/36426946/monocyte-macrophage-heterogeneity-during-skin-wound-healing-in-mice
#30
JOURNAL ARTICLE
Jingbo Pang, Mark Maienschein-Cline, Timothy J Koh
Monocytes (Mos)/macrophages (Mϕs) orchestrate biological processes critical for efficient skin wound healing. However, current understanding of skin wound Mo/Mϕ heterogeneity is limited by traditional experimental approaches such as flow cytometry and immunohistochemistry. Therefore, we sought to more fully explore Mo/Mϕ heterogeneity and associated state transitions during the course of excisional skin wound healing in mice using single-cell RNA sequencing. The live CD45+CD11b+Ly6G- cells were isolated from skin wounds of C57BL/6 mice on days 3, 6, and 10 postinjury and captured using the 10x Genomics Chromium platform...
November 15, 2022: Journal of Immunology
https://read.qxmd.com/read/36420091/knockdown-of-adra2a-increases-secretion-of-growth-factors-and-wound-healing-ability-in-diabetic-adipose-derived-stem-cells
#31
JOURNAL ARTICLE
Xiangyuan Zhao, Yong Zhang, Xinzhen Zuo, Shubai Wang, Xiao Dong
Studies showed that compared to normal adipose-derived stem cells (ASCs), ASCs from type 2 diabetic (T2D) mice were less effective in treating diabetic cutaneous wounds. However, the mechanisms remain unclear. Our transcriptomic profiling comparison showed that the expression of α 2A-adrenergic receptor (Adra2a) was significantly increased in ASCs from T2D mice (T2D ASCs). Therefore, the purpose of this study was to investigate whether the elevated Adra2a is involved in the diminished wound-healing capabilities of T2D ASCs...
2022: Stem Cells International
https://read.qxmd.com/read/36350668/examination-of-epigenetic-inhibitor-zebularine-in-treatment-of-skin-wounds-in-healthy-and-diabetic-mice
#32
JOURNAL ARTICLE
Piotr Sass, Paweł Sosnowski, Jolanta Kamińska, Milena Deptuła, Aneta Skoniecka, Jacek Zieliński, Sylwia Rodziewicz-Motowidło, Michał Pikuła, Paweł Sachadyn
DNA methyltransferase inhibitor zebularine was proven to induce regeneration in the ear pinna in mice. We utilized a dorsal skin wound model to further evaluate this epigenetic inhibitor in wound healing. Full-thickness excisional wounds were made on the dorsum of 2 and 10-month-old healthy BALB/c and 3 and 8-month-old diabetic (db/db) mice, followed by topical or intraperitoneal zebularine delivery. Depending on the strain, age, dose, and delivery, the zebularine treatments either had no effect or accelerated or delayed wound closure...
November 9, 2022: Journal of Tissue Engineering and Regenerative Medicine
https://read.qxmd.com/read/36180223/monocyte-macrophage-heterogeneity-during-skin-wound-healing-in-mice
#33
JOURNAL ARTICLE
Jingbo Pang, Mark Maienschein-Cline, Timothy J Koh
Monocytes (Mos)/macrophages (Mϕs) orchestrate biological processes critical for efficient skin wound healing. However, current understanding of skin wound Mo/Mϕ heterogeneity is limited by traditional experimental approaches such as flow cytometry and immunohistochemistry. Therefore, we sought to more fully explore Mo/Mϕ heterogeneity and associated state transitions during the course of excisional skin wound healing in mice using single-cell RNA sequencing. The live CD45+ CD11b+ Ly6G- cells were isolated from skin wounds of C57BL/6 mice on days 3, 6, and 10 postinjury and captured using the 10x Genomics Chromium platform...
September 30, 2022: Journal of Immunology
https://read.qxmd.com/read/36176609/cell-loaded-hydrogel-containing-ag-doped-bioactive-glass-ceramic-nanoparticles-as-skin-substitute-antibacterial-properties-immune-response-and-scarless-cutaneous-wound-regeneration
#34
JOURNAL ARTICLE
Esmaeel Sharifi, Seyede Athar Sadati, Satar Yousefiasl, Rossella Sartorius, Mahdi Zafari, Leila Rezakhani, Morteza Alizadeh, Ehsan Nazarzadeh Zare, Shadi Omidghaemi, Fatemeh Ghanavatinejad, Mohammad-Saeid Jami, Erfan Salahinejad, Hadi Samadian, Ana Cláudia Paiva-Santos, Piergiuseppe De Berardinis, Abbas Shafiee, Franklin R Tay, Samiramis Pourmotabed, Pooyan Makvandi
An ideal tissue-engineered dermal substitute should possess angiogenesis potential to promote wound healing, antibacterial activity to relieve the bacterial burden on skin, as well as sufficient porosity for air and moisture exchange. In light of this, a glass-ceramic (GC) has been incorporated into chitosan and gelatin electrospun nanofibers (240-360 nm), which MEFs were loaded on it for healing acceleration. The GC was doped with silver to improve the antibacterial activity. The bioactive nanofibrous scaffolds demonstrated antibacterial and superior antibiofilm activities against Gram-negative and Gram-positive bacteria...
September 2022: Bioengineering & Translational Medicine
https://read.qxmd.com/read/36170111/preparation-of-a-murine-oral-palate-wound-healing-model
#35
JOURNAL ARTICLE
Trevor R Leonardo, Lin Chen, Luisa A DiPietro
The oral cavity is a highly regenerative epithelial tissue that results in minimal scarring after injury. This protocol describes the preparation of a mouse palate wound model. The protocol includes steps to place an excisional wound on the mouse palate, followed by harvesting of wound tissue and bone decalcification. We detail how to overcome the technical challenge of limited anatomical space, avoid damaging the nasal cavity, manage bleeding, and collect tissue for downstream genomic or immunohistochemical analysis...
September 27, 2022: STAR protocols
https://read.qxmd.com/read/36067266/escherichia-coli-bara-uvry-regulates-the-pks-island-and-kills-staphylococci-via-the-genotoxin-colibactin-during-interspecies-competition
#36
JOURNAL ARTICLE
Jun Jie Wong, Foo Kiong Ho, Pei Yi Choo, Kelvin K L Chong, Chee Meng Benjamin Ho, Ramesh Neelakandan, Damien Keogh, Timothy Barkham, John Chen, Chuan Fa Liu, Kimberly A Kline
Wound infections are often polymicrobial in nature, biofilm associated and therefore tolerant to antibiotic therapy, and associated with delayed healing. Escherichia coli and Staphylococcus aureus are among the most frequently cultured pathogens from wound infections. However, little is known about the frequency or consequence of E. coli and S. aureus polymicrobial interactions during wound infections. Here we show that E. coli kills Staphylococci, including S. aureus, both in vitro and in a mouse excisional wound model via the genotoxin, colibactin...
September 6, 2022: PLoS Pathogens
https://read.qxmd.com/read/35958918/study-of-the-antimicrobial-activity-of-the-chinese-dong-ethnic-minority-medicine-madeng-ai
#37
JOURNAL ARTICLE
Zhenrong Tang, Yannan Zhao, Zaiqi Zhang, Huan Yue, Dan Wang, Shengchun Liu, Hua Tang
The overuse of antibiotics has contributed to the emergence of multidrug-resistant bacteria, which poses a challenging task for clinical therapy. Thus, new agents with antibiotic efficacy against multidrug-resistant infections are needed. The traditional Dong ethnic minority medicines have emerged as a new source for prodrug selection. Among them, Madeng'ai ( Potentillafreyniana Bornm) is widely used by the folk for anti-infection and wound healing, although the mechanisms remain unclear. In this study, the antimicrobial activities of Dong medicine Madeng'ai were evaluated both in vitro and i n vivo ...
2022: Evidence-based Complementary and Alternative Medicine: ECAM
https://read.qxmd.com/read/35892090/microrna-466-and-microrna-200-increase-endothelial-permeability-in-hyperglycemia-by-targeting-claudin-5
#38
JOURNAL ARTICLE
Marisa Kujawa, Megan O'Meara, Hainan Li, Liping Xu, Sai Pranathi Meda Venkata, Huong Nguyen, Morgan Minjares, Kezhong Zhang, Jie-Mei Wang
Endothelial cell (EC) permeability is essential to vascular homeostasis in diabetes. MicroRNAs are critical gene regulators whose roles in the EC permeability have yet to be characterized. This study aims to examine the change in cell permeability induced by miR-200 and miR-466 in ECs. Human aortic ECs and dermal microvascular ECs from healthy subjects and type 2 diabetic patients were used. Our in vitro experiments unveiled higher expressions of miR-200 family members and miR-466 in diabetic ECs and in healthy ECs when exposed to high glucose...
September 13, 2022: Molecular Therapy. Nucleic Acids
https://read.qxmd.com/read/35805190/selective-ablation-of-bcl11a-in-epidermal-keratinocytes-alters-skin-homeostasis-and-accelerates-excisional-wound-healing-in-vivo
#39
JOURNAL ARTICLE
Nilika Bhattacharya, Arup K Indra, Gitali Ganguli-Indra
Transcriptional regulator BCL11A plays a crucial role in coordinating a suite of developmental processes including skin morphogenesis, barrier functions and lipid metabolism. There is little or no reports so far documenting the role of BCL11A in postnatal adult skin homeostasis and in the physiological process of tissue repair and regeneration. The current study establishes for the first time the In Vivo role of epidermal BCL11A in maintaining adult epidermal homeostasis and as a negative regulator of cutaneous wound healing...
July 3, 2022: Cells
https://read.qxmd.com/read/35718003/comparative-studies-on-the-effect-of-pig-adipose-derived-stem-cells-pascs-preconditioned-with-hypoxia-or-normoxia-on-skin-wound-healing-in-mice
#40
JOURNAL ARTICLE
Joanna Wiśniewska, Magda Słyszewska, Marta Kopcewicz, Katarzyna Walendzik, Sylwia Machcińska, Karolina Stałanowska, Barbara Gawrońska-Kozak
Adipose-derived stem cells (ASCs) from human and animal fat have emerged as therapeutic alternatives for damaged tissues. Pre-conditioning of ASCs with hypoxia results in their functional enhancement, which might facilitate the process of healing. However, there is still a critical need for large-scale preclinical studies to reinforce the translation of these findings into clinical practice for humans and in veterinary medicine. Here, we adapted a full-thickness excisional skin wound mouse model to evaluate and compare the effect of pig adipose-derived stem cells (pASCs) cultured under normoxia (pASCs-Nor) or hypoxia (pASCs-Hyp) on the healing process...
September 1, 2022: Experimental Cell Research
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