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Excisional Wound Healing In The Mouse

Kamal Hany Hussein, Hani Nasser Abdelhamid, Xiaodong Zou, Heung-Myong Woo
In the present study, we investigated the applications of ultrasonicated graphene oxide (UGO) for bone regeneration and skin wound healing. Ultrasonication of a GO suspension increased the dispersion and stability (by increasing the zeta potential) of the GO suspension. UGO has fewer oxygen-containing groups but still displays excellent water dispersion. The UGO supension showed high biocompatibility for human fetal osteoblast (hFOB cells), human endothelial cells (EA.hy 926 cells), and mouse embryonic fibroblasts...
January 1, 2019: Materials Science & Engineering. C, Materials for Biological Applications
Nicole Edwards, Denis Feliers, Qingwei Zhao, Randolph Stone, Robert Christy, Xingguo Cheng
Chronic wounds complicated by diabetes are a significant clinical issue, and their occurrence is expected to continue to rise due to an increased prevalence of diabetes mellitus, especially type 2 diabetes. Diabetic wounds frequently lead to nonhealing ulcers, and often eventually result in limb amputation due to the high risk of infection of the chronic wound. Here, we present a tissue-engineered treatment that combines a novel electrochemically deposited collagen wound matrix and human adipose-derived stem cells...
October 2018: Journal of Biomaterials Applications
Yun-Ui Bae, Youlim Son, Chang-Hyun Kim, Kwang Seok Kim, Se Hee Hyun, Hyun Goo Woo, Byul A Jee, Jun-Hyuk Choi, Hoon-Ki Sung, Hyung-Chul Choi, So Young Park, Ju-Hyun Bae, Kyung-Oh Doh, Jae-Ryong Kim
Senescent cells accumulate in various tissues over time, and contribute to tissue dysfunction and aging-associated phenotypes. Accumulating evidence suggests that cellular senescence can be inhibited through pharmacological intervention, as well as through treatment with soluble factors derived from embryonic stem cells (ESCs). In an attempt to investigate the anti-senescence factors secreted by ESCs, we analyzed mouse ESC-derived extracellular miRNAs in conditioned medium (CM) via miRNA array analysis. We selected mmu-miR-291a-3p as a putative anti-senescence factor via bioinformatics analysis...
September 15, 2018: Journals of Gerontology. Series A, Biological Sciences and Medical Sciences
Corrie L Gallant-Behm, Joseph Piper, Brent A Dickinson, Christina M Dalby, Linda A Pestano, Aimee L Jackson
There is a strong unmet need for new therapeutics to accelerate wound healing across both chronic and acute indications. It is well established that local tissue hypoxia, vascular insufficiency, and/or insufficient angiogenesis contribute to inadequate wound repair in the context of diabetic foot ulcers as well as to other chronic wounds such as venous stasis and pressure ulcers. microRNA-92a-3p (miR-92a) is a potent antiangiogenic miRNA whose inhibition has led to increases in angiogenesis in multiple organ systems, resulting in an improvement in function following myocardial infarction, limb ischemia, vascular injury, and bone fracture...
August 17, 2018: Wound Repair and Regeneration
John Mark McLain, Wateen H Alami, Zachary T Glovak, Chris R Cooley, Susan J Burke, J Jason Collier, Helen A Baghdoyan, Michael D Karlstad, Ralph Lydic
Study Objectives: This study tested the hypothesis that sleep fragmentation (SF) delays wound healing in obese B6.BKS(D)-Leprdb/J (db/db) mice with impaired leptin signaling and type 2 diabetes compared with wild-type C57BL/6J (B6) mice. Methods: Adult male mice (n = 34) were anesthetized and bilateral full-thickness excisional wounds were created on the back of each mouse. Half of the db/db and B6 mice were housed in SF cages equipped with a bar that moved across the cage floor every 2 min, 12 hr/day for 23 days...
November 1, 2018: Sleep
Walison N Silva, Caroline Leonel, Pedro H D M Prazeres, Isadora F G Sena, Daniel A P Guerra, Debora Heller, Ivana M A Diniz, Vitor Fortuna, Akiva Mintz, Alexander Birbrair
Dermal wound healing is the process of repairing and remodeling skin following injury. Delayed or aberrant cutaneous healing poses a challenge for the health care system. The lack of detailed understanding of cellular and molecular mechanisms involved in this process hampers the development of effective targeted treatments. In a recent study, Parfejevs et al.-using state-of-the-art technologies, including in vivo sophisticated Cre/loxP techniques in combination with a mouse model of excisional cutaneous wounding-reveal that Schwann cells induce adult dermal wound healing...
August 11, 2018: Wound Repair and Regeneration
Ji Young Oh, Han Na Suh, Gee Euhn Choi, Hyun Jik Lee, Young Hyun Jung, So Hee Ko, Jun Sung Kim, Chang Woo Chae, Chang-Kyu Lee, Ho Jae Han
BACKGROUND AND PURPOSE: The sonic hedgehog pathway (Shh) plays a central role in maintaining stem cell function and behaviour in various processes related to self-renewal and tissue regeneration. However, the therapeutic effect of Shh on mouse embryonic stem cells (mESCs) has not yet been clearly elucidated. Thus, we investigated the effect of Shh on the regulation of mESC behaviour as well as the effect of Shh-pretreated mESCs in skin wound healing. EXPERIMENTAL APPROACH: The underlying mechanisms of Shh signalling pathway in growth and motility of mESCs were investigated using Western blot analysis, a cell proliferation assay and cell migration assay...
September 2018: British Journal of Pharmacology
Ji Young Oh, Gee Euhn Choi, Hyun Jik Lee, Young Hyun Jung, So Hee Ko, Chang Woo Chae, Jun Sung Kim, Seo Yihl Kim, Jae Ryong Lim, Chang-Kyu Lee, Ho Jae Han
BACKGROUND/AIMS: Glucose plays an important role in stem cell fate determination and behaviors. However, it is still not known how glucose contributes to the precise molecular mechanisms responsible for stem cell migration. Thus, we investigate the effect of glucose on the regulation of the human umbilical cord blood-derived mesenchymal stem cell (hUCB-MSC) migration, and analyze the mechanism accompanied by this effect. METHODS: Western blot analysis, wound healing migration assays, immunoprecipitation, and chromatin immunoprecipitation assay were performed to investigate the effect of high glucose on hUCB-MSC migration...
2018: Cellular Physiology and Biochemistry
Nicholas G Cogan, Alana P Mellers, Bhavi N Patel, Brett D Powell, Manu Aggarwal, Kathleen M Harper, Michael Blaber
We present a mathematical model to quantify parameters of mouse excisional wound healing from photographic data. The equation is a piecewise linear function in log scale that includes key parameters of initial wound radius (R0 ), an initial wound stasis phase (Ti ), and time to wound closure (Tc ); subsequently, these terms permit calculation of a latter active proliferative phase (Tp ), and the healing rate (HR) during this active phase. A daily photographic record of wound healing (utilizing 6 mm diameter splinted excisional wounds) permits the necessary sampling for robust parameter refinement...
March 2018: Wound Repair and Regeneration
B Kurt, N Bilge, M Sözmen, U Aydın, T Önyay, I Özaydın
Wound healing requires cells that increase both collagen production as a result of inflammatory events and regeneration of epithelial tissue. The Plantago species of herbs have been used in traditional treatment of skin disorders and infectious diseases, and digestive, respiratory, reproductive and circulatory conditions. We investigated the efficacy of different concentrations of Plantago lanceolata L. extract (PLE) for wound healing owing to its anti-inflammatory, anti-bacterial, anti-fungal, anti-oxidant, anti-ulcerative, analgesic and immunomodulatory properties...
2018: Biotechnic & Histochemistry: Official Publication of the Biological Stain Commission
Antoine L Carre, Michael S Hu, Aaron W James, Kenichiro Kawai, Michael G Galvez, Michael T Longaker, H Peter Lorenz
BACKGROUND: Acute wound healing is a dynamic process that results in the formation of scar tissue. The mechanisms of this process are not well understood; numerous signaling pathways are thought to play a major role. Here, the authors have identified β-catenin-dependent Wnt signaling as an early acute-phase reactant in acute wound healing and scar formation. METHODS: The authors created 6-mm full-thickness excisional cutaneous wounds on adult β-catenin-dependent Wnt signal (BAT-gal) reporter mice...
March 2018: Plastic and Reconstructive Surgery
Satoshi Nunomura, Yasuhiro Nanri, Masahiro Ogawa, Kazuhiko Arima, Yasutaka Mitamura, Tomohito Yoshihara, Hidetoshi Hasuwa, Simon J Conway, Kenji Izuhara
Periostin is a matricellular protein involved in development, maintenance, and regulation of tissues and organs via by binding to cell surface integrin receptors. Pathologically, periostin plays an important role in the process of wound healing: as a deficiency of the Postn gene delays wound closure and periostin is consistently up-regulated in response to injury and skin diseases. However, the functional role of elevated periostin in the process of wound healing has not been tested. In this study, we generated Postn-transgenic mice under the control of the CAG promoter/enhancer to investigate the effects of constitutive overexpression of full length periostin during its pathophysiological roles...
January 2018: Wound Repair and Regeneration
Si-Qian Gao, Chen Chang, Xiao-Qian Niu, Long-Jian Li, Yan Zhang, Jian-Qing Gao
To investigate the effects of Hydroxysafflor Yellow A (HSYA), which is derived from safflower, on the proliferation, migration and angiogenesis of cells in vitro and its potential efficacy in vivo when topically applied to a diabetic wound. Human umbilical vein endothelial cells (HUVECs) and mouse macrophage cells (RAW264.7) were used to evaluate angiogenesis and anti-inflammatory activities, respectively. The influence of HSYA on the wound scratch assay was investigated in keratinocytes. A splinted excisional wound model in rats with TIDM induced by streptozotocin was used to assess the effects of wound healing...
March 15, 2018: European Journal of Pharmacology
Michael S Hu, Justin Cheng, Mimi R Borrelli, Tripp Leavitt, Graham G Walmsley, Elizabeth R Zielins, Wan Xing Hong, Alexander T M Cheung, Dominik Duscher, Zeshaan N Maan, Dre M Irizarry, Brad Stephan, Fereydoun Don Parsa, Derrick C Wan, Geoffrey C Gurtner, Hermann Peter Lorenz, Michael T Longaker
Objective: Splinting full-thickness cutaneous wounds in mice has allowed for a humanized model of wound healing. Delineating the epithelial edge and assessing time to closure of these healing wounds via macroscopic visualization have remained a challenge. Approach: Double transgenic mice were created by crossbreeding K14-Cre and ROSAmT/mG reporter mice. Full-thickness excisional wounds were created in K14-Cre/ROSAmT/mG mice (n = 5) and imaged using both normal and fluorescent light on the day of surgery, and every other postoperative day (POD) until wound healing was complete...
January 1, 2018: Advances in Wound Care
Christian F Guerrero-Juarez, Aliaksandr A Astrowski, Rabi Murad, Christina T Dang, Vera O Shatrova, Aksana Astrowskaja, Chae Ho Lim, Raul Ramos, Xiaojie Wang, Yuchen Liu, Hye-Lim Lee, Kim T Pham, Tsai-Ching Hsi, Ji Won Oh, Daniel Crocker, Ali Mortazavi, Mayumi Ito, Maksim V Plikus
Large excisional wounds in mice prominently regenerate new hair follicles (HFs) and fat, yet humans are deficient for this regenerative behavior. Currently, wound-induced regeneration remains a clinically desirable, but only partially understood phenomenon. We show that large excisional wounds in rats across seven strains fail to regenerate new HFs. We compared wound transcriptomes between mice and rats at the time of scab detachment, which coincides with the onset of HF regeneration in mice. In both species, wound dermis and epidermis share core dermal and epidermal transcriptional programs, respectively, yet prominent interspecies differences exist...
June 2018: Journal of Investigative Dermatology
Tatiana V Boyko, Rakesh Bam, Dadi Jiang, Zhen Wang, Namrata Bhatia, Misha C Tran, Michael T Longaker, Albert C Koong, George P Yang
Wound healing is characterized by the production of large amounts of protein necessary to replace lost cellular mass and extracellular matrix. The unfolded protein response (UPR) is an important adaptive cellular response to increased protein synthesis. One of the main components of the UPR is IRE1, an endoplasmic reticulum transmembrane protein with endonuclease activity that produces the activated form of the transcription factor XBP1. Using luciferase reporter mice for Xbp1 splicing, we showed that IRE1 was up-regulated during excisional wound healing at the time in wound healing consistent with that of the proliferative phase, when the majority of protein synthesis for cellular proliferation and matrix deposition occurs...
November 2017: Wound Repair and Regeneration
Suneel Kumar, Martin L Yarmush, Biraja C Dash, Henry C Hsia, Francois Berthiaume
Pressure ulcers (PUs) are common debilitating complications of traumatic spinal cord injury (SCI) and tend to occur in soft tissues around bony prominences. There is, however, little known about the impact of SCI on skin wound healing because of the lack of suitable animal models for studies in controlled experimental settings. Herein, we describe a reproducible and clinically relevant mouse model of PUs in the context of complete SCI. Adult male mice (BALB/c) were subjected to thoracic (T9-T10) complete SCI...
March 15, 2018: Journal of Neurotrauma
Kelvin Kian Long Chong, Wei Hong Tay, Baptiste Janela, Adeline Mei Hui Yong, Tze Horng Liew, Leigh Madden, Damien Keogh, Timothy Mark Sebastian Barkham, Florent Ginhoux, David Laurence Becker, Kimberly A Kline
Enterococcus faecalis is one of the most frequently isolated bacterial species in wounds yet little is known about its pathogenic mechanisms in this setting. Here, we used a mouse wound excisional model to characterize the infection dynamics of E faecalis and show that infected wounds result in 2 different states depending on the initial inoculum. Low-dose inocula were associated with short-term, low-titer colonization whereas high-dose inocula were associated with acute bacterial replication and long-term persistence...
December 19, 2017: Journal of Infectious Diseases
Weirong Xiao, Hua Tang, Meng Wu, Yangying Liao, Ke Li, Lan Li, Xiaopeng Xu
Skin injury affects millions of people via the uncontrolled inflammation and infection. Many cellular components including fibroblasts and signaling pathways such as transforming growth factor-β (TGF-β) were activated to facilitate the wound healing to repair injured tissues. C57BL/6 female mice were divided into control and ozone oil treated groups. Excisional wounds were made on the dorsal skin and the fibroblasts were isolated from granulation tissues. The skin injured mouse model revealed that ozone oil could significantly decrease the wound area and accelerate wound healing compared with control group...
December 22, 2017: Bioscience Reports
Mingliang Sun, Yunfan He, Tao Zhou, Pan Zhang, Jianhua Gao, Feng Lu
Mesenchymal stem cells are an attractive cell type for cytotherapy in wound healing. The authors recently developed a novel, adipose-tissue-derived, injectable extracellular matrix/stromal vascular fraction gel (ECM/SVF-gel) for stem cell therapy. This study was designed to assess the therapeutic effects of ECM/SVF-gel on wound healing and potential mechanisms. ECM/SVF-gel was prepared for use in nude mouse excisional wound healing model. An SVF cell suspension and phosphate-buffered saline injection served as the control...
2017: BioMed Research International
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