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Rat burn

Yu Chen, Haiying Qiu, Miheng Dong, Bin Cheng, Yiguang Jin, Zongrui Tong, Puwang Li, Sidong Li, Ziming Yang
Improving the antibacterial properties of membrane wound dressings of natural polymers is crucial. Iodine is an important safe inorganic antibacterial agent, but was confined in the composition with polymer membranes due to the challenges of homogeneity and stability during drying. In the present work, iodine was complexed with hydroxylated lecithin (HL) to improve its stability and complexing efficiency for the composition with carboxymethly chitosan/sodium alginate. With the aid of microwave drying, hydroxylated lecithin complexed iodine/carboxymethly chitosan/sodium alginate (HLI/CMCS/SA) composite membranes with homogeneously distributions of HLI, high contents of activated iodine, good mechanical and swelling properties, proper water vapor permeability, pH controllable iodine release and excellent antibacterial properties were prepared...
February 15, 2019: Carbohydrate Polymers
Gholamhassan Haddadi, Akbar Abbaszadeh, Mohammad Amin Mosleh-Shirazi, Mohammad Ali Okhovat, Ashkan Salajeghe, Zhila Ghorbani
Introduction: Skin is highly prone to radiation damage. Radiation burn is defined as damage to the skin or other biological tissues induced by radiofrequency or ionizing radiation. Vascular endothelial growth factor (VEGF) is a heparin-binded pro-angiogenic factor. Flavonoids belong to a family of polyphenol chemical compounds that are frequently present in fruits and vegetables. Hesperidin is an agent belonging to the flavonoid family. The aim of this study is to investigate whether hesperidin can affect the VEGF gene expression in rat skin following gamma irradiation or not...
December 2018: Journal of Cancer Research and Therapeutics
Shelby L Blaes, Caitlin A Orsini, Hannah M Holik, Toneisha D Stubbs, Shandera N Ferguson, Sara C Heshmati, Matthew M Bruner, Shannon C Wall, Marcelo Febo, Adriaan W Bruijnzeel, Jennifer L Bizon, Barry Setlow
Numerous preclinical studies show that acute cannabinoid administration impairs cognitive performance. Almost all of this research has employed cannabinoid injections, however, whereas smoking is the preferred route of cannabis administration in humans. The goal of these experiments was to systematically determine how acute exposure to cannabis smoke affects working memory performance in a rat model. Adult male (n = 15) and female (n = 16) Long-Evans rats were trained in a food-motivated delayed response working memory task...
December 3, 2018: Neurobiology of Learning and Memory
Mariana Vigiola Cruz, Bonnie C Carney, Jenna N Luker, Kyle W Monger, Juan Sebastian Vazquez, Lauren T Moffatt, Laura S Johnson, Jeffrey W Shupp
BACKGROUND: A complex inflammatory response mediates the systemic effects of burn shock. Disruption of the endothelial glycocalyx causes shedding of structural glycoproteins, primarily syndecan-1 (SDC-1), leading to endothelial dysfunction. These effects may be mitigated by resuscitative interventions. MATERIALS AND METHODS: Sprague-Dawley rats were used to create small, medium, and large burns and uninjured controls. Three different intravenous resuscitation protocols were applied within each group: Lactated Ringer's (LR) alone, LR plus fresh frozen plasma (FFP), or LR plus albumin...
January 2019: Journal of Surgical Research
Sheng Liu, Hong-Ze Chen, Zheng-Dong Xu, Fei Wang, Haoshu Fang, Ophelia Bellanfante, Xu-Lin Chen
BACKGROUND: Inflammatory response triggered by high mobility group box-1 (HMGB1) protein and oxidative stress play critical roles in the intestinal injury after severe burn. Sodium butyrate, a histone deacetylase inhibitor, has potential anti-inflammatory properties, inhibiting the expression of inflammatory mediators such as HMGB1 in diverse diseases. This study was designed to investigate the effects of sodium butyrate on severe burn plus delayed resuscitation-induced intestine injury, intestinal expressions of HMGB1 and intracellular adhesion molecule-1 (ICAM-1), oxidative stress, and signal transduction pathway changes in rats...
October 25, 2018: Burns: Journal of the International Society for Burn Injuries
Ronghua Yang, Jingru Wang, Ziheng Zhou, Shaohai Qi, Shubin Ruan, Zepeng Lin, Qi Xin, Yan Lin, Xiaodong Chen, Julin Xie
Local transplantation of stem cells has therapeutic effects on skin damage but cannot provide satisfactory wound healing. Studies on the mechanisms underlying the therapeutic effects of stem cells on skin wound healing will be needed. Hence, in the present study, we explored the role of Caveolin-1 in epidermal stem cells (EpiSCs) in the modulation of wound healing. We first isolated EpiSCs from mouse skin tissues and established stable EpiSCs with overexpression of Caveolin-1 using a lentiviral construct. We then evaluated the epidermal growth factor (EGF)-induced cell proliferation ability using cell counting Kit-8 (CCK-8) assay and assessed EpiSC pluripotency by examining Nanog mRNA levels in EpiSCs...
November 23, 2018: Developmental Biology
Gang-Yi Fan, Juin-Hong Cherng, Shu-Jen Chang, Raju Poongodi, Chih-Hsin Wang, Yi-Wen Wang, Yao-Horng Wang, Cheng-Che Liu, En Meng
Wound healing is a dynamic repair process and is the most complex biological process in human life. In response to burn injury, alterations in biological pathways impair the inflammation response, resulting in delayed wound healing. Impaired wound healing frequently occurs in patients with diabetes leading to unfavorable outcomes such as amputation. Hence, dressings having beneficial effect in promoting burn wound repair are needed. However, studies on burn wound treatment are limited due to lack of proper animal models...
November 6, 2018: Journal of Visualized Experiments: JoVE
Andrey Temnov, Tatyana Astrelina, Konstantin Rogov, Boris Moroz, Vladimir Lebedev, Tamara Nasonova, Alla Lyrshchikova, Olga Dobrynina, Yury Deshevoy, Alexander Melerzanov, Augustinus Bader, Apurva Mishra, Shibashish Giri, Valeriy Boyarintsev, Alexander Trofimenko, Andrey Bushmanov, Alexander Samoylov
Background: Mesenchymal stem cells based paracrine bioactive factors that deploy their task as an essential mechanism, but their efficiency for skin regeneration still requires clarification. Methods: The mesenchymal stem cell-based paracrine factors were administered by subcutaneous injection of 0.5 mL peptides (general protein 8 mg/mL). These were performed after radiation on different days like the first, third, sixth, eighth, and 10th. To determine the consequences, we performed photography, planimetry, and preclinical test each week after 15 days of radiation...
2018: Stem Cells and Cloning: Advances and Applications
Ashley N Guillory, Robert P Clayton, Anesh Prasai, Jayson W Jay, Michael Wetzel, Amina El Ayadi, David N Herndon, Celeste C Finnerty
BACKGROUND: It has been previously shown that anesthesia and analgesia can affect outcomes in the rat burn model and that buprenorphine alleviated pain without drastically altering the outcomes of interest. Recently, the use of a sustained release (SR) formulation of buprenorphine has been promoted over conventional buprenorphine. In this study, we assessed whether buprenorphine-SR altered hemodynamic parameters in our rat model of severe burn injury. MATERIALS AND METHODS: Adult male Sprague-Dawley rats were randomized to receive either conventional buprenorphine (0...
December 2018: Journal of Surgical Research
Pengfei Liang, Bimei Jiang, Yuanbin Li, Zhenguo Liu, Pihong Zhang, Minghua Zhang, Xiaoyuan Huang, Xianzhong Xiao
Our previous study demonstrated that angiogenesis increased during the recovery of heat-denatured endothelial cells. However, the mechanism is still unclear. This study aimed to investigate the relation of autophagy and angiogenesis during the recovery of heat-denatured endothelial cells. A rat deep partial-thickness burn model and heat-denatured human umbilical vein endothelial cells (HUVECs) model (52 °C for 35 s) were used. Autophagy increased significantly in the dermis and HUVECs in a time-dependent manner after heat denaturation and recovery for 2-5 days...
November 19, 2018: Cell Death & Disease
Masayuki Otawara, Maedeh Roushan, Xiao Wang, Felix Ellett, Yong-Ming Yu, Daniel Irimia
Cell-free DNA (cf-DNA) concentration in human plasma is often increased after burn and trauma injuries. Two major sources of cf-DNA are the parenchymal cells damaged by the injury and various circulating cells indirectly altered by the response to injury. The cf-DNA originating from neutrophils, also known as circulating neutrophil extracellular traps (cNETs), is of notable interest because cNETs have been associated with pathological processes in other conditions, including cancer, autoimmunity, etc. Both intact chromatin and oligonucleotides, which are the by-product of cf-DNA degradation, are assumed to contribute to the cf-DNA in patients...
November 19, 2018: Scientific Reports
Ruhma Mahmood, Azra Mehmood, Mahmood S Choudhery, Sana Javaid Awan, Shaheen N Khan, Sheikh Riazuddin
Conventional approaches can repair minor skin injuries; however, severe burn injuries require innovative approaches for efficient and better wound repair. Recent studies indicate that stem-cell-based regenerative therapies can restore severe damaged skin both structurally and functionally. The current study aims to evaluate the wound healing potential of skin substitute derived from human neonatal stem cells (hNSCs) using a severe burn injury rat model. AECs (Amniotic epithelial cells) and MSCs (mesenchymal stem cells) were isolated from placenta (a source of neonatal stem cells) by explant culture method...
November 16, 2018: Cell Biology International
Ștefania Marin, Mădălina Georgiana Albu Kaya, Mihaela Violeta Ghica, Cristina Dinu-Pîrvu, Lăcrămioara Popa, Denisa Ioana Udeanu, Geanina Mihai, Marius Enachescu
The aim of this study is to design, develop and evaluate new biohybrid sponges based on polymers (collagen and polyvinyl alcohol) with and without indomethacin as anti-inflammatory drug model to be used for tissue regeneration in wound healing. Type I fibrillar collagen in the form of a gel and different concentrations of polyvinyl alcohol were mixed together to prepare composite gels. Both control samples, without indomethacin and with indomethacin, were obtained. All samples were crosslinked with glutaraldehyde...
November 9, 2018: Pharmaceutics
Feng Shao, AiJun Yang, Dong Mei Yu, Juan Wang, Xue Gong, Hong Xiao Tian
Bio-synthesis of nano-metal oxide particles is gaining lot of significance and recommended as promising substitute not only physical methods but also chemical methods. Here in, we demonstrate the nano-zinc oxide (nano-ZnO) particles were successfully prepared by an eco-friendly process using plant Barleria gibsoni (B. gibsoni) aqueous leaf extract. The water leaf extract of B. gibsoni responsible for not only reducing source but also protective agent. The prepared nano-ZnO particles were studied by UV-Vis diffuse reflectance (UV-DRS), Photoluminescence (PL), Fourier transform (FT-IR) infrared spectroscopy, X-ray diffraction spectroscopy (X-RD), transmission electron microscopy (TEM), thermal stability was studied by thermogravimetric and differential thermal (TG-DTA) analysis and particle size by zeta sizer, dynamic light scattering (DLS)...
October 18, 2018: Journal of Photochemistry and Photobiology. B, Biology
Yonghui Yu, Longlong Yang, Shaofang Han, Yushou Wu, Lingying Liu, Yang Chang, Xiaoteng Wang, Jiake Chai
INTRODUCTION: Cell autophagy is an important material recycling process and is involved in regulating many vital activities under both physiological and pathological conditions. However, the mechanism of autophagy regulating burn-induced skeletal muscle wasting still needs to be elucidated. METHODS: The rat burn model with 30% total body surface area and L6 cell line were used in this study. An immunofluorescence assay was used to detect autophagic levels. miRNA array and real-time PCR were employed to measure miR-190b levels, and its influence on PHLPP1 protein translation was estimated using luciferase reporter assay...
November 6, 2018: Shock
Swaminathan Jiji, Sivalingam Udhayakumar, Chellan Rose, Chellappa Muralidharan, Krishna Kadirvelu
Bacterial cellulose is well known for its excellent contributions in biomedical applications due to its superior properties. However the lack of antimicrobial property restricts its use in wound healing. To address the complications in third degree burns, thymol enriched bacterial cellulose hydrogel (BCT) was developed in this study. The incorporation of thymol into bacterial cellulose along with its chemical and thermal changes were investigated by FTIR, TGA and DSC respectively. Antimicrobial studies revealed that BCT possess excellent biocidal activity against burn specific pathogens...
October 29, 2018: International Journal of Biological Macromolecules
M J Fatemi, M Momeni, A Tavakoli, T Bagheri, A Hosseini, S Araghi, F Ranjpoor, A Zavareh
Several dermal products have been introduced to substitute dermal tissues. In this study we review the effects of these products on repairing third-degree burn wounds and managing complications in animal specimens. Using an interventional approach, rats were randomly assigned to four groups (G1 to G4). Two wounds were created on the back of each rat. An open wound was left on the back of rats in G1; in G2, wounds were covered with a thick rat derived-ADM product and overlying thin skin graft; on G3 rats, similar third degree ulcers were made with one ulcer covered with harvested thin skin graft...
June 30, 2018: Annals of Burns and Fire Disasters
Parastoo Pourali, Behrooz Yahyaei
PURPOSE: Although alginate has been known to be a good wound dressing, it does not have antimicrobial properties, has low availability, and is expensive. In order to overcome these problems, this study was conducted, where the extraction of this material from an available small factory Pseudomonas aeruginosa and the improvement of its wound healing property by its combination with herb extract, Alhagi maurorum, done. METHODS: Nineteen P. aeruginosa strains were isolated and identified from burned skin and one isolated strain with the highest ability of alginate production was selected...
October 29, 2018: Dermatologic Therapy
Ya Butko, V Ulanova, O Tkacheva, O Mishchenko
The objective of our research was histological evaluation of therapeutic effects in case of burn injury in rats. Experiment was conducted on 84 test rats with burn injury of III-A stage. Therapeutical effect of studied medicines was evaluated using histological methods. Results achived gave evidence that on 18-th day of treatment with ceremids cream and dexpanthenol with ceramids cream the level granulation tissue maturing increased by 1,4 and 1,7 times and intensity of burn injury epithelization rose by 1,5 and 1,9 times in comparison with non-treated animals...
September 2018: Georgian Medical News
Madhu Verma, Arun Nanda, Yatendra Kumar
PURPOSE: The purpose of the study was formulation development, optimization and evaluation of a Self-Emulsifying Drug Delivery System (SEDDS) of Simvastatin (SIM) for improvement in dissolution and bioavailability of SIM. Solubility enhancement of Biopharmaceutical Classification System (BCS) Class-II drugs is a burning topic and attracting various publications and patents regarding different strategies employed for improvement of dissolution viz., USOO5340591A (Solid dispersion), US005472954A, US005646131A (complexation), USOO5858410A (Nanosuspensions), USOO5874029A (micronization) US 2008...
October 22, 2018: Recent Patents on Drug Delivery & Formulation
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