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OipA helicobacter pylori

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https://www.readbyqxmd.com/read/30288255/phenotypic-analysis-of-antibiotic-resistance-and-genotypic-study-of-the-vaca-caga-icea-oipa-and-baba-genotypes-of-the-helicobacter-pylori-strains-isolated-from-raw-milk
#1
Reza Ranjbar, Farid Yadollahi Farsani, Farhad Safarpoor Dehkordi
Background: Foods with animal origins and particularly milk play a considerable role in transmission of Helicobacter pylori . The current study was performed to assess phenotypic characters of antibiotic resistance and genotyping pattern of vacA , cagA , iceA , oipA and babA2 alleles amongst the H. pylori strains isolated from raw milk. Methods: Six-hundred and thirty raw milk samples were collected and cultured on Wilkins Chalgren anaerobe media. Antibiotic resistance and genotyping patterns were studied using disk diffusion and PCR, respectively...
2018: Antimicrobial Resistance and Infection Control
https://www.readbyqxmd.com/read/29796347/-helicobacter-pylori-genetic-diversification-in-the-mongolian-gerbil-model
#2
Amber C Beckett, John T Loh, Abha Chopra, Shay Leary, Aung Soe Lin, Wyatt J McDonnell, Beverly R E A Dixon, Jennifer M Noto, Dawn A Israel, Richard M Peek, Simon Mallal, Holly M Scott Algood, Timothy L Cover
Helicobacter pylori requires genetic agility to infect new hosts and establish long-term colonization of changing gastric environments. In this study, we analyzed H. pylori genetic adaptation in the Mongolian gerbil model. This model is of particular interest because H. pylori -infected gerbils develop a high level of gastric inflammation and often develop gastric adenocarcinoma or gastric ulceration. We analyzed the whole genome sequences of H. pylori strains cultured from experimentally infected gerbils, in comparison to the genome sequence of the input strain...
2018: PeerJ
https://www.readbyqxmd.com/read/29452293/genetic-diversity-and-functional-analysis-of-oipa-gene-in-association-with-other-virulence-factors-among-helicobacter-pylori-isolates-from-iranian-patients-with-different-gastric-diseases
#3
Nastaran Farzi, Abbas Yadegar, Hamid Asadzadeh Aghdaei, Yoshio Yamaoka, Mohammad Reza Zali
Helicobacter pylori (H. pylori) is one of the most genetically diverse bacterial pathogens that persistently colonizes the human gastric epithelium. This remarkable genomic plasticity may act as a driving force for successful adaptation and persistence of the bacteria in the harsh gastric environment. Outer inflammatory protein A (OipA) encoded by oipA gene (HP0638/hopH) is a member of the outer membrane proteins (OMPs) of H. pylori involved in induction of IL-8 secretion and is associated with development of peptic ulcer and gastric cancer...
June 2018: Infection, Genetics and Evolution
https://www.readbyqxmd.com/read/29405158/molecular-identification-and-detection-of-virulent-factors-in-helicobacter-pylori-from-gastric-biopsy-samples-of-patients-attended-at-assam-medical-college-and-hospital-dibrugarh-assam-india
#4
Anisha Sarma, Lahari Saikia, Ratna Kanta Bhuyan, Ezaz Hussain
The present study aimed to detect the major virulence determinants of Helicobacter pylori, the gastric bacteria by polymerase chain reaction from genomic DNA of 314 gastric biopsies from dyspeptic patients in 2015-2016 through upper gastrointestinal endoscopy. In 153 cases out of 314, the high prevalence of oipA gene followed by cagA-vacA s1 m1 combined genotypes was found in mostly gastritis/duodenitis patients followed by the peptic ulcer and normal patients. Therefore, the clinical significance of the virulence markers of H...
October 2017: Indian Journal of Medical Microbiology
https://www.readbyqxmd.com/read/29040466/outer-inflammatory-protein-a-oipa-of-helicobacter-pylori-is-regulated-by-host-cell-contact-and-mediates-caga-translocation-and-interleukin-8-response-only-in-the-presence-of-a-functional-cag-pathogenicity-island-type-iv-secretion-system
#5
Danielle N Horridge, Allison A Begley, June Kim, Neeraja Aravindan, Kexin Fan, Mark H Forsyth
OipA is a phase-variable virulence factor of Helicobacter pylori. Mutations in oipA to turn the gene phase on in a cag pathogenicity island (PAI)-negative strain of H. pylori (J68) or phase off in a cag PAI-positive strain (26695) demonstrated that phase on oipA alleles in both strains had both increased oipA mRNA and human gastric adenocarcinoma (AGS) cell adherence compared to isogenic oipA phase off mutants. An oipA phase off mutant of H. pylori 26695 demonstrated decreased IL-8 secretion by AGS cells and failure to translocate the cag PAI effector CagA...
November 30, 2017: Pathogens and Disease
https://www.readbyqxmd.com/read/28892894/low-rate-of-baba2-genotype-among-iranian-helicobacter-pylori-clinical-isolates
#6
Maryam Sohrabi, Reza Khashei, Mahvash Alizadeh, Mohammad-Kazem Hosseini Asl, Mohammad-Ali Nejati, Mahintaj Dara, Abdollah Bazargani
INTRODUCTION: The Blood Group Antigen-Binding Adhesion (babA), Outer Inflammatory Protein (oipA) and Sialic Acid-Binding Adhesin (sabA) as outer membrane proteins involved in Helicobacter pylori adherence to gastric mucosa have been suggested to have a role in the pathogenesis. AIM: To investigate the frequency of H. pylori isolates babA2, oipA and sabA genes in Iranian dyspeptic patients. MATERIALS AND METHODS: DNAs were extracted from H...
July 2017: Journal of Clinical and Diagnostic Research: JCDR
https://www.readbyqxmd.com/read/28808292/binding-of-the-helicobacter-pylori-oipa-causes-apoptosis-of-host-cells-via-modulation-of-bax-bcl-2-levels
#7
Omid Teymournejad, Ashraf Mohabati Mobarez, Zuhair Mohammad Hassan, Amin Talebi Bezmin Abadi
The H. pylori outer inflammatory protein A (OipA) is an outer membrane protein that contributes to gastric inflammation. OipA is believed to affect intra-cellular signalling and modulate the host signalling pathways. The aim of the current study was to clarify the role of OipA in H. pylori pathogenesis and its effect on host cell signalling pathways. To this end, the oipA gene was isolated and inserted into cloning and expression vectors. The recombinant plasmid was transferred into an expression host to produce OipA, which was subsequently purified by affinity chromatography and used for antibody production...
August 14, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28776327/helicobacter-pylori-outer-inflammatory-protein-a-oipa-suppresses-apoptosis-of-ags-gastric-cells-in-vitro
#8
Anis Rageh Al-Maleki, Mun Fai Loke, Sook Yin Lui, Nur Siti Khadijah Ramli, Yalda Khosravi, Chow Goon Ng, Gopinath Venkatraman, Khean-Lee Goh, Bow Ho, Jamuna Vadivelu
Outer inflammatory protein A (OipA) is an important virulence factor associated with gastric cancer and ulcer development; however, the results have not been well established and turned out to be controversial. This study aims to elucidate the role of OipA in Helicobacter pylori infection using clinical strains harbouring oipA "on" and "off" motifs. Proteomics analysis was performed on AGS cell pre-infection and postinfection with H. pylori oipA "on" and "off" strains, using liquid chromatography/mass spectrometry...
December 2017: Cellular Microbiology
https://www.readbyqxmd.com/read/28338646/type-iv-secretion-and-signal-transduction-of-helicobacter-pylori-caga-through-interactions-with-host-cell-receptors
#9
REVIEW
Steffen Backert, Nicole Tegtmeyer
Helicobacter pylori is a highly successful human bacterium, which is exceptionally equipped to persistently inhabit the human stomach. Colonization by this pathogen is associated with gastric disorders ranging from chronic gastritis and peptic ulcers to cancer. Highly virulent H. pylori strains express the well-established adhesins BabA/B, SabA, AlpA/B, OipA, and HopQ, and a type IV secretion system (T4SS) encoded by the cag pathogenicity island (PAI). The adhesins ascertain intimate bacterial contact to gastric epithelial cells, while the T4SS represents an extracellular pilus-like structure for the translocation of the effector protein CagA...
March 24, 2017: Toxins
https://www.readbyqxmd.com/read/28263510/the-role-of-outer-inflammatory-protein-a-oipa-in-vaccination-of-thec57bl-6-mouse-model-infected-by-helicobacter-pylori
#10
Mohaddese Mahboubi, Tahereh Falsafi, Majid Sadeghizadeh, Fatemeh Mahjoub
BACKGROUND/AIM: Outer inflammatory protein A (OipA) is an important adhesin of Helicobacter pylori. Our goal was to assess the role of OipA in protection of C57BL/6 mice against H. pylori. MATERIALS AND METHODS: C57BL/6 mice were mucosally immunized with recombinant OipA protein, OipA + propolis, propolis, and phosphate-buffered saline. After vaccination, anti-OipA IgA was measured. Mice were challenged three times with 5 × 10(7) CFU of the H. pylori B19 strain...
February 27, 2017: Turkish Journal of Medical Sciences
https://www.readbyqxmd.com/read/28215588/prevalence-of-helicobacter-pylori-vaca-caga-cage-oipa-icea-baba2-and-babb-genotypes-in-iranian-dyspeptic-patients
#11
Hossein Dabiri, Fereshteh Jafari, Kaveh Baghaei, Leila Shokrzadeh, Saeed Abdi, Mohamad Amin Pourhoseingholi, Alireza Mohammadzadeh
There is diversity in clinical outcome of Helicobacter pylori infection in different regions. Microbial, host and environmental factors seem to be reason of such variation. Considering microbial factors, we studied H. pylori genotypes and their association with clinical feature of the infection. Overall 160 H. pylori-positive patients were enrolled in this study. Clinical information and biopsy were collected from each patient. The presence of the major virulence genes were determined by PCR. Regardless to clinical outcomes, vacA, cagA, cagE, oipA, iceA1, babA2 and babB genes was positive in 100%, 69%, 51%, 55%, 26%,78% and 28% of 160 strains respectively...
April 2017: Microbial Pathogenesis
https://www.readbyqxmd.com/read/28194462/designing-an-efficient-multi-epitope-oral-vaccine-against-helicobacter-pylori-using-immunoinformatics-and-structural-vaccinology-approaches
#12
Navid Nezafat, Mahboobeh Eslami, Manica Negahdaripour, Mohammad Reza Rahbar, Younes Ghasemi
Helicobacter pylori is the cunning bacterium that can live in the stomachs of many people without any symptoms, but gradually can lead to gastric cancer. Due to various obstacles, which are related to anti-H. pylori antibiotic therapy, recently developing an anti-H. pylori vaccine has attracted more attention. In this study, different immunoinformatics and computational vaccinology approaches were employed to design an efficient multi-epitope oral vaccine against H. pylori. Our multi-epitope vaccine is composed of heat labile enterotoxin IIc B (LT-IIc) that is used as a mucosal adjuvant to enhance vaccine immunogenicity for oral immunization, cartilage oligomeric matrix protein (COMP) to increase vaccine stability in acidic pH of gut, one experimentally protective antigen, OipA, and two hypothetical protective antigens, HP0487 and HP0906, and "CTGKSC" peptide motif that target epithelial microfold cells (M cells) to enhance vaccine uptake from the gut barrier...
March 28, 2017: Molecular BioSystems
https://www.readbyqxmd.com/read/28033692/helicobacter-pylori-and-its-virulence-factors-effect-on-serum-oxidative-dna-damages-in-adults-with-dyspepsia
#13
Heshmat Shahi, Rasoul Bahreiny, Somayeh Reiisi
Helicobacter Pylori infection is a common gastrointestinal infection that can cause pathological effects, increase oxidative stress and induce an inflammatory response in gastric mucosa. Inflammatory aspects may prompt the production of radical oxygen substance (ROS) which may damage cells and release 8-hydroxydyoxyguanosine (8-OHdG) to serum. In this study, we evaluate the prevalence of H. pylori virulence factors and the association between serum level of 8-OHdG, H. pylori infection, and its various virulence factors...
November 2016: Acta Medica Iranica
https://www.readbyqxmd.com/read/27819260/combination-of-oipa-baba-and-saba-as-candidate-biomarkers-for-predicting-helicobacter-pylori-related-gastric-cancer
#14
Yu-Lin Su, Hsiang-Ling Huang, Bo-Shih Huang, Po-Chung Chen, Chien-Sheng Chen, Hong-Long Wang, Pin-Hsin Lin, Meng-Shu Chieh, Jiunn-Jong Wu, Jyh-Chin Yang, Lu-Ping Chow
Helicobacter pylori (H. pylori ) infection is a major cause of chronic gastritis and is highly related to duodenal ulcer (DU) and gastric cancer (GC). To identify H. pylori-related GC biomarkers with high seropositivity in GC patients, differences in levels of protein expression between H. pylori from GC and DU patients were analyzed by isobaric tag for relative and absolute quantitation (iTRAQ). In total, 99 proteins showed increased expression (>1.5-fold) in GC patients compared to DU patients, and 40 of these proteins were categorized by KEGG pathway...
November 7, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27419049/helicobacter-pylori-isolated-from-iranian-drinking-water-vaca-caga-icea-oipa-and-baba2-genotype-status-and-antimicrobial-resistance-properties
#15
Reza Ranjbar, Faham Khamesipour, Nematollah Jonaidi-Jafari, Ebrahim Rahimi
Despite the clinical importance of Helicobacter pylori in human gastric disorders, its exact route of transmission is still uncertain. Based on the contentious hypothesis and findings of previous investigations, water may play an important role in the transmission of H. pylori to humans. This study was carried out to investigate the vacA, cagA, oipA, iceA and babA2 genotype status and antimicrobial resistance properties of H. pylori strains isolated from the drinking water samples of four major provinces in Iran...
May 2016: FEBS Open Bio
https://www.readbyqxmd.com/read/26970903/helicobacter-pylori-in-bottled-mineral-water-genotyping-and-antimicrobial-resistance-properties
#16
Reza Ranjbar, Faham Khamesipour, Nematollah Jonaidi-Jafari, Ebrahim Rahimi
BACKGROUND: Up to now, fecal-oral and oral-oral are the most commonly known routes for transmission of H. pylori, therefore, contaminated water can play an important role in transmission of H. pylori to humans. Genotyping using virulence markers of H. pylori is one of the best approaches to study the correlations between H. pylori isolates from different samples. The present research was carried out to study the vacA, cagA, cagE, oipA, iceA and babA2 genotyping and antimicrobial resistance properties of H...
March 12, 2016: BMC Microbiology
https://www.readbyqxmd.com/read/26792758/vaca-caga-icea-and-oipa-genotypes-status-and-antimicrobial-resistance-properties-of-helicobacter-pylori-isolated-from-various-types-of-ready-to-eat-foods
#17
Behsan Hemmatinezhad, Hassan Momtaz, Ebrahim Rahimi
BACKGROUND: Despite the high clinical standing of Helicobacter pylori, its exact routes of transmission and origin have not been determined. Based on the contentious hypothesis, foods play an important roles in the transmission of H. pylori to humans. The present study was carried out to investigate the vacA, cagA, oipA and iceA genotypes status of H. pylori isolated from the various types of ready to eat foods. METHODS: A total of 550 ready to eat food samples were cultured and tested...
January 20, 2016: Annals of Clinical Microbiology and Antimicrobials
https://www.readbyqxmd.com/read/26442711/helicobacter-pylori-virulence-genes-in-the-five-largest-islands-of-indonesia
#18
Muhammad Miftahussurur, Ari Fahrial Syam, Dadang Makmun, Iswan Abbas Nusi, Lukman Hakim Zein, Zulkhairi, Fardah Akil, Willi Brodus Uswan, David Simanjuntak, Tomohisa Uchida, Pangestu Adi, Amanda Pitarini Utari, Yudith Annisa Ayu Rezkitha, Phawinee Subsomwong, Nasronudin, Yoshio Yamaoka
BACKGROUND: It remains unclear whether the low incidence of gastric cancer in Indonesia is due to low infection rates only or is also related to low Helicobacter pylori pathogenicity. We collected H. pylori strains from the five largest islands in Indonesia and evaluated genetic virulence factors. METHODS: The genotypes of H. pylori virulence factors were determined by polymerase chain reaction (PCR)-based sequencing. Histological severity of the gastric mucosa was classified into 4 grades, according to the updated Sydney system...
2015: Gut Pathogens
https://www.readbyqxmd.com/read/26338221/resistance-to-clarithromycin-and-genotypes-in-helicobacter-pylori-strains-isolated-in-sicily
#19
Teresa Fasciana, Cinzia Calà, Celestino Bonura, Enza Di Carlo, Domenica Matranga, Giuseppe Scarpulla, Michele Manganaro, Salvatore Camilleri, Anna Giammanco
The resistance of Helicobacter pylori strains to clarithromycin is increasing in several developed countries and their association with a genetic pattern circulation has been variously explained as related to different geographical areas. In this study we have reported: the prevalence of the resistance of H. pylori, isolated in Sicily, to clarithromycin; the principal point of mutation associated with this resistance; and the more frequent association between resistance to clarithromycin and cagA, the EPIYA motif, and the vacA and oipA genes...
November 2015: Journal of Medical Microbiology
https://www.readbyqxmd.com/read/26287606/multiplex-pcr-based-screening-and-computational-modeling-of-virulence-factors-and-t-cell-mediated-immunity-in-helicobacter-pylori-infections-for-accurate-clinical-diagnosis
#20
Sinem Oktem-Okullu, Arzu Tiftikci, Murat Saruc, Bahattin Cicek, Eser Vardareli, Nurdan Tozun, Tanil Kocagoz, Ugur Sezerman, Ahmet Sinan Yavuz, Ayca Sayi-Yazgan
The outcome of H. pylori infection is closely related with bacteria's virulence factors and host immune response. The association between T cells and H. pylori infection has been identified, but the effects of the nine major H. pylori specific virulence factors; cagA, vacA, oipA, babA, hpaA, napA, dupA, ureA, ureB on T cell response in H. pylori infected patients have not been fully elucidated. We developed a multiplex- PCR assay to detect nine H. pylori virulence genes with in a three PCR reactions. Also, the expression levels of Th1, Th17 and Treg cell specific cytokines and transcription factors were detected by using qRT-PCR assays...
2015: PloS One
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