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https://www.readbyqxmd.com/read/30304770/optimization-of-growth-and-carotenoid-production-by-haloferax-mediterranei-using-response-surface-methodology
#1
Zaida Montero-Lobato, Adrián Ramos-Merchante, Juan Luis Fuentes, Ana Sayago, Ángeles Fernández-Recamales, Rosa María Martínez-Espinosa, José María Vega, Carlos Vílchez, Inés Garbayo
Haloferax mediterranei produces C50 carotenoids that have strong antioxidant properties. The response surface methodology (RSM) tool helps to accurately analyze the most suitable conditions to maximize C50 carotenoids production by haloarchaea. The effects of temperature (15⁻50 °C), pH (4-10), and salinity (5⁻28% NaCl ( w / v )) on the growth and carotenoid content of H. mediterranei were analyzed using the RSM approach. Growth was determined by measuring the turbidity at 600 nm. To determine the carotenoid content, harvested cells were lysed by freeze/thawing, then re-suspended in acetone and the total carotenoid content determined by measuring the absorbance at 494 nm...
October 9, 2018: Marine Drugs
https://www.readbyqxmd.com/read/30245679/sialic-acid-like-sugars-in-archaea-legionaminic-acid-biosynthesis-in-the-halophile-halorubrum-sp-pv6
#2
Marianna Zaretsky, Elina Roine, Jerry Eichler
N-glycosylation is a post-translational modification that occurs in all three domains. In Archaea, however, N-linked glycans present a degree of compositional diversity not observed in either Eukarya or Bacteria. As such, it is surprising that nonulosonic acids (NulOs), nine-carbon sugars that include sialic acids, pseudaminic acids, and legionaminic acids, are routinely detected as components of protein-linked glycans in Eukarya and Bacteria but not in Archaea. In the following, we report that the N-linked glycan attached to the S-layer glycoprotein of the haloarchaea Halorubrum sp...
2018: Frontiers in Microbiology
https://www.readbyqxmd.com/read/30232361/overexpression-of-different-types-of-microbial-rhodopsins-with-a-highly-expressible-bacteriorhodopsin-from-haloarcula-marismortui-as-a-single-protein-in-e-coli
#3
Cheng-Hong Tu, Hsiu-Ping Yi, Shiang-Yuan Hsieh, Hong-Syuan Lin, Chii-Shen Yang
Microbial rhodopsins (M-Rho) are found in Archaea, Bacteria and some species of Eukarya and serve as light-driven ion pumps or mediate phototaxis responses in various biological systems. We previously reported an expression system using a highly expressible mutant, D94N-HmBRI (HEBR) from Haloarcula marismortui, as a leading tag to assist in the expression of membrane proteins that were otherwise difficult to express in E. coli. In this study, we show a universal strategy for the expression of two M-Rho proteins, either the same or different types, as one fusion protein with the HEBR system...
September 19, 2018: Scientific Reports
https://www.readbyqxmd.com/read/30224495/modified-mevalonate-pathway-of-the-archaeon-aeropyrum-pernix-proceeds-via-trans-anhydromevalonate-5-phosphate
#4
Hajime Hayakawa, Kento Motoyama, Fumiaki Sobue, Tomokazu Ito, Hiroshi Kawaide, Tohru Yoshimura, Hisashi Hemmi
The modified mevalonate pathway is believed to be the upstream biosynthetic route for isoprenoids in general archaea. The partially identified pathway has been proposed to explain a mystery surrounding the lack of phosphomevalonate kinase and diphosphomevalonate decarboxylase by the discovery of a conserved enzyme, isopentenyl phosphate kinase. Phosphomevalonate decarboxylase was considered to be the missing link that would fill the vacancy in the pathway between mevalonate 5-phosphate and isopentenyl phosphate...
October 2, 2018: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/30125086/impact-of-haloarchaea-on-speciation-of-uranium-a-multi-spectroscopic-approach
#5
Miriam Bader, Andre Rossberg, Robin Steudtner, Björn Drobot, Kay Großmann, Matthias Schmidt, Niculina Musat, Thorsten Stumpf, Atsushi Ikeda-Ohno, Andrea Cherkouk
Haloarchaea represent a predominant part of the microbial community in rock salt, which can serve as host rock for the disposal of high level radioactive waste. However, knowledge is missing about how Haloarchaea interact with radionuclides. Here, we used a combination of spectroscopic and microscopic methods to study the interactions of an extremely halophilic archaeon with uranium, one of the major radionuclides in high level radioactive waste, on a molecular level. The obtained results show that Halobacterium noricense DSM 15987T influences uranium speciation as a function of uranium concentration and incubation time...
August 20, 2018: Environmental Science & Technology
https://www.readbyqxmd.com/read/30123209/halolysin-spta-a-serine-protease-contributes-to-growth-phase-transition-of-haloarchaeon-natrinema-sp-j7-2-and-its-expression-involves-cooperative-action-of-multiple-cis-regulatory-elements
#6
Moran Li, Jing Yin, Sha Mei, Xuhong Wang, Xiao-Feng Tang, Bing Tang
Many haloarchaea produce extracellular subtilisin-like proteases (halolysins) during late log phase; however, the physiological function and regulatory mechanism of growth phase-dependent production of halolysins are unknown. Halolysin SptA, the major extracellular protease of Natrinema sp. J7-2, is capable of intracellular self-activation to affect haloarchaeal growth. Here, we report that deletion of sptA leads to loss of extracellular and intracellular protease activities against azocasein and/or suc-AAPF-pNA, as well as a change in growth-phase transition of the haloarchaeon...
2018: Frontiers in Microbiology
https://www.readbyqxmd.com/read/30098005/low-temperature-raman-spectroscopy-reveals-small-chromophore-distortion-in-primary-photointermediate-of-proteorhodopsin
#7
LETTER
Tomotsumi Fujisawa, Masahiro Abe, Jun Tamogami, Takashi Kikukawa, Naoki Kamo, Masashi Unno
Proteorhodopsin (PR) is a microbial rhodopsin functioning as a light-driven proton pump in aquatic bacteria. We performed low-temperature Raman measurements of PR to obtain the structure of the primary photoproduct, the K intermediate (PRK ). PRK showed the hydrogen-out-of-plane modes that are much less intense than those of bacteriorhodopsin as the prototypical light-driven proton pump from haloarchaea. The present results reveal the significantly relaxed chromophore structure in PRK , which can be coupled to the slow kinetics of the K intermediate...
August 11, 2018: FEBS Letters
https://www.readbyqxmd.com/read/29925429/genomic-variation-and-biogeography-of-antarctic-haloarchaea
#8
Bernhard Tschitschko, Susanne Erdmann, Matthew Z DeMaere, Simon Roux, Pratibha Panwar, Michelle A Allen, Timothy J Williams, Sarah Brazendale, Alyce M Hancock, Emiley A Eloe-Fadrosh, Ricardo Cavicchioli
BACKGROUND: The genomes of halophilic archaea (haloarchaea) often comprise multiple replicons. Genomic variation in haloarchaea has been linked to viral infection pressure and, in the case of Antarctic communities, can be caused by intergenera gene exchange. To expand understanding of genome variation and biogeography of Antarctic haloarchaea, here we assessed genomic variation between two strains of Halorubrum lacusprofundi that were isolated from Antarctic hypersaline lakes from different regions (Vestfold Hills and Rauer Islands)...
June 20, 2018: Microbiome
https://www.readbyqxmd.com/read/29888524/proteomic-study-of-the-exponential-stationary-growth-phase-transition-in-the-haloarchaea-natrialba-magadii-and-haloferax-volcanii
#9
Micaela Cerletti, María Ines Giménez, Christian Tröetschel, Celeste D' Alessandro, Ansgar Poetsch, Rosana Ester De Castro, Roberto A Paggi
The dynamic changes that take place along the phases of microbial growth (lag, exponential, stationary, and death) have been widely studied in bacteria at the molecular and cellular levels, but little is known for archaea. In this study, a high-throughput approach was used to analyze and compare the proteomes of two haloarchaea during exponential and stationary growth: the neutrophilic Haloferax volcanii and the alkaliphilic Natrialba magadii. Almost 2000 proteins were identified in each species (≈50% of the predicted proteome)...
July 2018: Proteomics
https://www.readbyqxmd.com/read/29880023/hundreds-of-novel-composite-genes-and-chimeric-genes-with-bacterial-origins-contributed-to-haloarchaeal-evolution
#10
Raphaël Méheust, Andrew K Watson, François-Joseph Lapointe, R Thane Papke, Philippe Lopez, Eric Bapteste
BACKGROUND: Haloarchaea, a major group of archaea, are able to metabolize sugars and to live in oxygenated salty environments. Their physiology and lifestyle strongly contrast with that of their archaeal ancestors. Amino acid optimizations, which lowered the isoelectric point of haloarchaeal proteins, and abundant lateral gene transfers from bacteria have been invoked to explain this deep evolutionary transition. We use network analyses to show that the evolution of novel genes exclusive to Haloarchaea also contributed to the evolution of this group...
June 7, 2018: Genome Biology
https://www.readbyqxmd.com/read/29778319/anti-infective-biomaterials-with-surface-decorated-tachyplesin-i
#11
Qiong Xue, Xiao-Bin Liu, Yeh-Hsing Lao, Lin-Ping Wu, Dong Wang, Zhen-Qiang Zuo, Jun-Yu Chen, Jing Hou, Yuan-Yuan Bei, Xue-Fei Wu, Kam W Leong, Hua Xiang, Jing Han
Implants decorated with antimicrobial peptides (AMPs) can prevent infection and reduce the risk of creating antibiotic resistance. Yet the restricted mobility of surficial AMP often compromises its activity. Here, we report a simple but effective strategy to allow a more flexible display of AMP on the biomaterial surface and demonstrate its efficacy for wound healing. The AMP, tachyplesin I (Tac), is tagged with the polyhydroxyalkanoate-granule-associated protein (PhaP) and immobilized on haloarchaea-produced poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBHV) via hydrophobic interaction...
September 2018: Biomaterials
https://www.readbyqxmd.com/read/29775706/purification-and-characterization-of-an-organic-solvent-tolerant-and-detergent-stable-lipase-from-haloferax-mediterranei-cncmm-50101
#12
Siham Akmoussi-Toumi, Souad Khemili-Talbi, Imen Ferioune, Salima Kebbouche-Gana
The present study investigates the purification and biochemical characterization of an extracellular lipase (HML) from Haloarchaea Haloferax mediterranei strain ATS1, isolated from the Sebkha (Medea, Algeria). The pure protein was obtained with ammonium sulfate precipitation (40-70%)-dialysis and UNO Q-6 FPLC, and characterized biochemically. Matrix assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF/MS) analysis revealed that the purified enzyme was a monomer, with a molecular mass of 45,011...
September 2018: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/29773867/novel-haloarchaeon-natrinema-thermophila-having-the-highest-growth-temperature-among-haloarchaea-with-a-large-genome-size
#13
Yeon Bee Kim, Joon Yong Kim, Hye Seon Song, Changsu Lee, Seung Woo Ahn, Se Hee Lee, Min Young Jung, Jin-Kyu Rhee, Juseok Kim, Dong-Wook Hyun, Jin-Woo Bae, Seong Woon Roh
Environmental temperature is one of the most important factors for the growth and survival of microorganisms. Here we describe a novel extremely halophilic archaeon (haloarchaea) designated as strain CBA1119T isolated from solar salt. Strain CBA1119T had the highest maximum and optimal growth temperatures (66 °C and 55 °C, respectively) and one of the largest genome sizes among haloarchaea (5.1 Mb). It also had the largest number of strain-specific pan-genome orthologous groups and unique pathways among members of the genus Natrinema in the class Halobacteria...
May 17, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29725727/using-a-portable-raman-spectrometer-to-detect-carotenoids-of-halophilic-prokaryotes-in-synthetic-inclusions-in-nacl-kcl-and-sulfates
#14
Jan Jehlička, Adam Culka, Lilly Mana, Aharon Oren
Cell suspensions of the haloarchaea Halorubrum sodomense and Halobacterium salinarum and the extremely halophilic bacterium Salinibacter ruber (Bacteroidetes) in saturated solutions of chlorides and sulfates (NaCl, KCl, MgSO4 ·7H2 O, K2 SO4 , and (NH4 )Al(SO4 )2 ·12H2 O) were left to evaporate to produce micrometric inclusions in laboratory-grown crystals. Raman spectra of these pinkish inclusions were obtained using a handheld Raman spectrometer with green excitation (532 nm). This portable instrument does not include any microscopic tool...
July 2018: Analytical and Bioanalytical Chemistry
https://www.readbyqxmd.com/read/29677156/divergent-roles-of-rpa-homologs-of-the-model-archaeon-halobacterium-salinarum-in-survival-of-dna-damage
#15
Jessica J Evans, Patrick E Gygli, Julienne McCaskill, Linda C DeVeaux
The haloarchaea are unusual in possessing genes for multiple homologs to the ubiquitous single-stranded DNA binding protein (SSB or replication protein A, RPA) found in all three domains of life. Halobacterium salinarum contains five homologs: two are eukaryotic in organization, two are prokaryotic and are encoded on the minichromosomes, and one is uniquely euryarchaeal. Radiation-resistant mutants previously isolated show upregulation of one of the eukaryotic-type RPA genes. Here, we have created deletions in the five RPA operons...
April 20, 2018: Genes
https://www.readbyqxmd.com/read/29595162/exploring-the-potential-of-halophilic-archaea-for-the-decolorization-of-azo-dyes
#16
Masoomeh Selseleh Hassan Kiadehi, Mohammad Ali Amoozegar, Sedigheh Asad, Maryam Siroosi
Azo dyes are being extensively used in textile industries, so finding a proper solution to decolorize them is of high importance. In order to find azo dye decolorizing strains among haloarchaea, which are well known for their tolerance to harsh environmental conditions, fifteen haloarchaeal strains were screened. Halogeometricum sp. strain A and Haloferax sp. strain B with the highest decolorization ability (95% and 91% for Remazol black B; both about 60% for Acid blue 161, respectively) were selected for further studies...
March 2018: Water Science and Technology: a Journal of the International Association on Water Pollution Research
https://www.readbyqxmd.com/read/29553064/presence-of-a-haloarchaeal-halorhodopsin-like-cl-pump-in-marine-bacteria
#17
Yu Nakajima, Takashi Tsukamoto, Yohei Kumagai, Yoshitoshi Ogura, Tetsuya Hayashi, Jaeho Song, Takashi Kikukawa, Makoto Demura, Kazuhiro Kogure, Yuki Sudo, Susumu Yoshizawa
Light-driven ion-pumping rhodopsins are widely distributed among bacteria, archaea, and eukaryotes in the euphotic zone of the aquatic environment. H+ -pumping rhodopsin (proteorhodopsin: PR), Na+ -pumping rhodopsin (NaR), and Cl- -pumping rhodopsin (ClR) have been found in marine bacteria, which suggests that these genes evolved independently in the ocean. Putative microbial rhodopsin genes were identified in the genome sequences of marine Cytophagia. In the present study, one of these genes was heterologously expressed in Escherichia coli cells and the rhodopsin protein named Rubricoccus marinus halorhodopsin (RmHR) was identified as a light-driven inward Cl- pump...
March 29, 2018: Microbes and Environments
https://www.readbyqxmd.com/read/29520450/haloarchaea-from-the-andean-puna-biological-role-in-the-energy-metabolism-of-arsenic
#18
Omar Federico Ordoñez, María Cecilia Rasuk, Mariana Noelia Soria, Manuel Contreras, María Eugenia Farías
Biofilms, microbial mats, and microbialites dwell under highly limiting conditions (high salinity, extreme aridity, pH, and elevated arsenic concentration) in the Andean Puna. Only recent pioneering studies have described the microbial diversity of different Altiplano lakes and revealed their unexpectedly diverse microbial communities. Arsenic metabolism is proposed to be an ancient mechanism to obtain energy by microorganisms. Members of Bacteria and Archaea are able to exploit arsenic as a bioenergetic substrate in either anaerobic arsenate respiration or chemolithotrophic growth on arsenite...
October 2018: Microbial Ecology
https://www.readbyqxmd.com/read/29518047/metagenomic-insights-into-the-phylogenetic-and-metabolic-diversity-of-the-prokaryotic-community-dwelling-in-hypersaline-soils-from-the-odiel-saltmarshes-sw-spain
#19
Blanca Vera-Gargallo, Antonio Ventosa
Hypersaline environments encompass aquatic and terrestrial habitats. While only a limited number of studies on the microbial diversity of saline soils have been carried out, hypersaline lakes and marine salterns have been thoroughly investigated, resulting in an aquatic-biased knowledge about life in hypersaline environments. To improve our understanding of the assemblage of microbes thriving in saline soils, we assessed the phylogenetic diversity and metabolic potential of the prokaryotic community of two hypersaline soils (with electrical conductivities of ~24 and 55 dS/m) from the Odiel saltmarshes (Spain) by metagenomics...
March 8, 2018: Genes
https://www.readbyqxmd.com/read/29463600/transcriptional-landscape-and-regulatory-roles-of-small-noncoding-rnas-in-the-oxidative-stress-response-of-the-haloarchaeon-haloferax-volcanii
#20
Diego Rivera Gelsinger, Jocelyne DiRuggiero
Haloarchaea in their natural environment are exposed to hypersalinity, intense solar radiation, and desiccation, all of which generate high levels of oxidative stress. Previous work has shown that haloarchaea are an order of magnitude more resistant to oxidative stress than most mesophilic organisms. Despite this resistance, the pathways haloarchaea use to respond to oxidative stress damage are similar to those of nonresistant organisms, suggesting that regulatory processes might be key to their robustness...
May 1, 2018: Journal of Bacteriology
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