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Biofilm wound

Seung-Hyun Noh, Shin-Woong Kim, Jae-Won Kim, Tae-Hoon Lee, Jae-Woon Nah, Young-Gi Lee, Mi-Kyung Kim, Yoshihiro Ito, Tae-Il Son
This study demonstrated the anti-adhesion and wound healing effect of a visible light curable anti-adhesion agent using an alginate derivative modified with a furfuryl moiety. Visible light-curable furfuryl alginate (F-Alg) was prepared in conjugation with alginate and furfurylamine by an amide coupling reaction, and the conjugated F-Alg was characterized by 1 H NMR analysis. The cytotoxicity, cell adhesion, and cell permeability of the F-Alg were evaluated for use in anti-adhesion applications. Drug immobilization and protein release were assessed to verify whether the alginate derivatives and drugs were photo-immobilized...
October 9, 2018: International Journal of Biological Macromolecules
Harshavardhan V Pawar, John Tetteh, Philip Debrah, Joshua S Boateng
Infected chronic wounds heal slowly, exhibiting prolonged inflammation, biofilm formation, bacterial resistance, high exudate and ineffectiveness of systemic antimicrobials. Composite dressings (films and wafers) comprising polyox/carrageenan (POL-CAR) and polyox/sodium alginate (POL-SA), loaded with diclofenac (DLF) and streptomycin (STP) were formulated and tested for antibacterial activity against 2 × 105  CFU/mL of Escherichia coli, Pseudomonas aeruginosa and Staphylococcus aureus representing infected chronic wounds and compared with marketed silver dressings...
October 6, 2018: International Journal of Biological Macromolecules
Md Imran Khan, Susanta Kumar Behera, Prajita Paul, Bhaskar Das, Mrutyunjay Suar, R Jayabalan, Derek Fawcett, Gerrard Eddy Jai Poinern, Suraj K Tripathy, Amrita Mishra
The aim of the present study is focused on the synthesis of Au@ZnO core-shell nanocomposites, where zinc oxide is overlaid on biogenic gold nanoparticles obtained from Hibiscus Sabdariffa plant extract. Optical property of nanocomposites is investigated using UV-visible spectroscopy and crystal structure has been determined using X-ray crystallography (XRD) technique. The presence of functional groups on the surface of Au@ZnO core-shell nanocomposites has been observed by Fourier transforms infrared (FTIR) spectroscopy...
October 5, 2018: Medical Microbiology and Immunology
Stefanie M Shiels, David J Tennent, Alicia L Lofgren, Joseph C Wenke
Infectious complications can reduce fracture healing rate. Broad spectrum antibiotics are commonly administered to prevent and treat musculoskeletal infections. Local antibiotics are applied to the wound site to increase therapeutic concentrations without increasing systemic toxicity however may hinder local tissue recovery. Rifampin has been shown to eradicate mature Staphylococcal biofilms and its use proven for treating musculoskeletal infections. In this study, a spontaneously healing defect model in a rat was used to investigate the impact rifampin powder has on endogenous bone healing in both a sterile and contaminated wound...
October 1, 2018: Journal of Orthopaedic Research: Official Publication of the Orthopaedic Research Society
Anne-Marie Salisbury, Dieter Mayer, Rui Chen, Steven L Percival
Objective: This study was set up to evaluate the efficacy of a concentrated surfactant-based wound dressing (with and without silver sulfadiazine [SSD]) on wound repair, by investigating their ability to enhance human dermal fibroblast proliferation and viability. In addition, the wound dressings were evaluated for their ability to suppress biofilms in a three-dimensional (3D) in vitro wound biofilm model and modulate the inflammatory cytokine interleukin-6 (IL-6) and tumor necrosis factor-alpha (TNFα). Approach: Problematic biofilms are well known to affect fibroblast and keratinocyte viability...
September 1, 2018: Advances in Wound Care
Mohamed S El Masry, Scott Chaffee, Piya Das Ghatak, Shomita S Mathew-Steiner, Amitava Das, Natalia Higuita-Castro, Sashwati Roy, Raafat A Anani, Chandan K Sen
Decellularized matrices of biologic tissue have performed well as wound care dressings. Extracellular matrix-based dressings are subject to rapid degradation by excessive protease activity at the wound environment. Stabilized, acellular, equine pericardial collagen matrix (sPCM) wound care dressing is flexible cross-linked proteolytic enzyme degradation resistant. sPCM was structurally characterized utilizing scanning electron and atomic force microscopy. In murine excisional wounds, sPCM was effective in mounting an acute inflammatory response...
September 27, 2018: FASEB Journal: Official Publication of the Federation of American Societies for Experimental Biology
W Kram, H Rebl, R Wyrwa, T Laube, A Zimpfer, M Maruschke, M Frank, B Vollmar, G Kundt, M Schnabelrauch, B Nebe, N Buchholz, O W Hakenberg
Ureteric stents have become an indispensable tool in the armamentarium of every urologist. However, they carry their own morbidity resulting mostly from infectious or abacterial fouling and biofilm formation, and/or urothelial hyperplastic reaction. All of these may interact and lead to clinical complications. Many different stent designs and coatings have been proposed. In this study, we focused on the effect of paclitaxel-coated stents on hyperplastic proliferation of ureteral tissue, using as example anastomotic strictures after ureteroureterostomy in a rat model...
September 26, 2018: Urolithiasis
Omar A Taha, Phillippa L Connerton, Ian F Connerton, Ayman El-Shibiny
The recorded growth in infection by multidrug resistant bacteria necessitates prompt efforts toward developing alternatives to antibiotics, such as bacteriophage therapy. Immuno-compromised patients with diabetes mellitus are particularly prone to foot infections by multidrug resistant Klebsiella pneumoniae , which may be compounded by chronic osteomyelitis. Bacteriophage ZCKP1, isolated from freshwater in Giza, Egypt, was tested in vitro to evaluate its lytic activity against a multidrug resistant K. pneumoniae KP/01, isolated from foot wound of a diabetic patient in Egypt...
2018: Frontiers in Microbiology
Mona Moniri, Amin Boroumand Moghaddam, Susan Azizi, Raha Abdul Rahim, Saad Wan Zuhainis, Mohammad Navaderi, Rosfarizan Mohamad
Background: In recent years, bacterial nanocellulose (BNC) based nanocomposites have been developed to promote healing property and antibacterial activity of BNC wound dressing. Molecular study can help to better understanding about interaction of genes and pathways involved in healing progression. Objectives: The aim of this study was to prepare bacterial nanocellulose/silver (BNC/Ag) nanocomposite films as ecofriendly wound dressing in order to assess their physical, cytotoxicity and antimicrobial properties...
2018: International Journal of Nanomedicine
Bahman Mirzaei, Somayeh Etemadian, Hamid Reza Goli, Sara Bahonar, Sanaz Amir Gholami, Parisima Karami, Mojgan Farhadi, Rahmatollah Tavakoli
Developing a strategy for making the alginate base hydrogel components against burned wound infections could be promising for healing the mentioned wounds followed by elimination of the biofilm forming bacteria colonization. Construction of an alginate based hydrogel and evaluating healing activities of the mentioned component as local ointment were the main objectives of the current study. Following the collection of the honey from three different provinces of Iran, the components and structures of the collected materials were analyzed taking advantage of INSO-92 procedure subsequently, antibacterial effect of diluted three different kinds of honey against wild-type bacterial species got evaluated via agar well diffusion method...
2018: International Journal of Burns and Trauma
Chintan Pansara, Wei Yee Chan, Ankitkumar Parikh, Darren J Trott, Tejal Mehta, Renuka Mishra, Sanjay Garg
Antimicrobial resistance at the infected site is a serious medical issue that increases patient morbidity and mortality. Silver has antibacterial activity associated with some dose dependent toxicity. Silver nanoparticles, due to larger surface area, have antibacterial properties which make them useful in the treatment of infections. Chitosan stabilised silver nanoparticles (CH-AgNP) were formulated and evaluated for minimal inhibitory concentration (MIC) and minimal bactericidal concentration (MBC) testing against Staphylococcus aureus ATCC 29213, Staphylococcus aureus ATCC 25923, Pseudomonas aeruginosa ATCC 27853, Escherichia coli ATCC 25922 and 20 methicillin-resistant S...
September 19, 2018: Journal of Pharmaceutical Sciences
Amal Gharamti, Zeina A Kanafani
Vascular graft infection is a devastating complication of vascular reconstructive surgery. The infection can occur early in the postoperative period and is largely due to intraoperative contamination or by contiguous extension from a nearby infection. It can also occur years after implantation. Staphylococci remain the most common organisms and biofilm production makes eradication difficult. Factors commonly reported to predispose to vascular graft infection are periodontal disease, nasal colonization with Staphylococcus aureus, bacteremia, certain graft characteristics, diabetes mellitus, postoperative hyperglycemia, location of the incision, wound infection, and emergency procedure...
September 18, 2018: Infectious Disease Clinics of North America
Ian MacNiven, Jason E Hsu, Moni B Neradilek, Frederick A Matsen
Background: Propionibacterium species are commonly cultured from specimens harvested at the time of revision shoulder arthroplasty. These bacteria reside in normal sebaceous glands, out of reach of surgical skin preparation. The arthroplasty incision transects these structures, which allows Propionibacterium to inoculate the wound and to potentially lead to the formation of a biofilm on the inserted implant. To help identify patients who are at increased risk for wound inoculation, we investigated whether preoperative cultures of the specimens from the unprepared skin surface were predictive of the results of intraoperative cultures of dermal wound-edge specimens obtained immediately after incision of the surgically prepared skin...
March 29, 2018: JB & JS open access
Hanne L P Tytgat, Franklin L Nobrega, John van der Oost, Willem M de Vos
Bacterial communities are known to impact human health and disease. Mixed species biofilms, mostly pathogenic in nature, have been observed in dental and gastric infections as well as in intestinal diseases, chronic gut wounds and colon cancer. Apart from the appendix, the presence of thick polymicrobial biofilms in the healthy gut mucosa is still debated. Polymicrobial biofilms containing potential pathogens appear to be an early-warning signal of developing disease and can be regarded as a tipping point between a healthy and a diseased state of the gut mucosa...
September 12, 2018: Trends in Microbiology
Sha Yang, Yun Yang, Sixin Cui, Ziqi Feng, Yuzhi Du, Zhen Song, Yanan Tong, Liuyang Yang, Zelin Wang, Hao Zeng, Quanming Zou, Hongwu Sun
Introduction: Methicillin-resistant Staphylococcus aureus (MRSA) is one of the most predominant and fatal pathogens at wound infection sites. MRSA is difficult to treat because of its antibiotic resistance and ability to form biofilms at the wound site. Methods: In this study, a novel nanoscale liquid film-forming system (LFFS) loaded with benzalkonium bromide was produced based on polyvinyl alcohol and chitosan. Results: This LFFS showed a faster and more potent effect against MRSA252 than benzalkonium bromide aqueous solution both in vitro and in vivo...
2018: International Journal of Nanomedicine
Zhuobin Xu, Zhiyue Qiu, Qi Liu, Yixin Huang, Dandan Li, Xinggui Shen, Kelong Fan, Juqun Xi, Yunhao Gu, Yan Tang, Jing Jiang, Jialei Xu, Jinzhi He, Xingfa Gao, Yuan Liu, Hyun Koo, Xiyun Yan, Lizeng Gao
The use of natural substance to ward off microbial infections has a long history. However, the large-scale production of natural extracts often reduces antibacterial potency, thus limiting practical applications. Here we present a strategy for converting natural organosulfur compounds into nano-iron sulfides that exhibit enhanced antibacterial activity. We show that compared to garlic-derived organosulfur compounds nano-iron sulfides exhibit an over 500-fold increase in antibacterial efficacy to kill several pathogenic and drug-resistant bacteria...
September 13, 2018: Nature Communications
Joey Karim Ead, Robert J Snyder, Jaime Wise, Cherison Cuffy, Hamed Jafary, Karen Fischborn
INTRODUCTION: When occurring together, pyoderma gangrenosum, severe acne, and hidradenitis suppurativa have been described as PASH syndrome. Due to the chronic autoinflammatory state existing in affected patients, PASH syndrome has been attributed to the dysregulation of wound healing. CASE REPORTS: Two cases are presented that demonstrate the paradigmatic clinical features of PASH syndrome and its potential link as an expanding spectrum of bacterial biofilm disorder...
August 2018: Wounds: a Compendium of Clinical Research and Practice
Xiaomei Dai, Yu Zhao, Yunjian Yu, Xuelei Chen, Xiaosong Wei, Xinge Zhang, Chaoxing Li
The chronic infection of humans by antibiotic-resistant bacteria and their related biofilm have, so far, not been properly addressed. In the present work, we developed a novel antibacterial nanoplatform showing the most efficient antibiotic-resistant bacteria inhibition and biofilm eradication. This particular formulation contains tobramycin-conjugated graphene oxide, for efficiently capturing bacteria through electrostatic interactions and eliminating bacteria as a "nano-knife", and copper sulphide nanoparticles for enhancing the photothermal and photodynamic properties...
September 13, 2018: Nanoscale
Patrick A Rühs, Flavian Storz, Yuly A López Gómez, Matthias Haug, Peter Fischer
Bacterial cellulose is a remarkable fibrous structural component of biofilms, as it forms a mechanically strong hydrogel with high water adsorption capabilities. Additionally, bacterial cellulose is biocompatible and therefore of potential interest for skin regeneration and wound healing applications. However, bacterial cellulose produced through conventional production processes at water-air interfaces lack macroporosity control, which is crucial for regenerative tissue applications. Here we demonstrate a straightforward and efficient approach to form a macroporous bacterial cellulose foam by foaming a mannitol-based media with a bacterial suspension of Gluconoacetobacter xylinus ...
2018: NPJ Biofilms and Microbiomes
Yunhui Zhang, Andrea Sass, Heleen Van Acker, Jasper Wille, Bruno Verhasselt, Filip Van Nieuwerburgh, Volkhard Kaever, Aurélie Crabbé, Tom Coenye
As one of the major pathogens in wound infections, Pseudomonas aeruginosa produces several virulence factors and forms biofilms; these processes are under the regulation of various quorum sensing (QS) systems. Therefore, QS has been regarded as a promising target to treat P. aeruginosa infections. In the present study, we evaluated the effect of the plant-derived QS inhibitor coumarin on P. aeruginosa biofilms and virulence. Coumarin inhibited QS in the P. aeruginosa QSIS2 biosensor strain, reduced protease and pyocyanin production, and inhibited biofilm formation in microtiter plates in different P...
2018: Frontiers in Microbiology
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