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https://www.readbyqxmd.com/read/30549385/molecular-epidemiology-of-mrsa-in-the-brazilian-primary-health-care-system
#1
Eliane Patricia Lino Pereira Franchi, Maria Rachel Nogueira Barreira, Natália de Sousa Lima Moreira da Costa, Danilo Flávio Moraes Riboli, Ligia Maria Abraão, Katheryne Benini Martins, Cassiano Victória, Maria de Lourdes Ribeiro de Souza da Cunha
OBJECTIVE: To evaluate the molecular epidemiology and to georeference Staphylococcus aureus isolated from wounds and nares of patients seen at Basic Health Units (BHUs) of a Brazilian city. METHODS: Observational, cross-sectional study conducted from 2010 to 2013. A total of 119 S. aureus strains isolated from the wounds and nares of 88 patients were studied. The isolates were characterized by identifying virulence genes encoding enterotoxins A-E, hemolysins α, β and δ, exfoliatins A, B and D, biofilm production, Panton-Valentine leukocidin and toxic shock syndrome toxin 1, and by pulsed-field gel electrophoresis(PFGE), multilocus sequence and spa typing...
December 14, 2018: Tropical Medicine & International Health: TM & IH
https://www.readbyqxmd.com/read/30523603/potential-properties-of-lactobacillus-plantarum-f-10-as-a-bio-control-strategy-for-wound-infections
#2
Tugce Onbas, Ozlem Osmanagaoglu, Fadime Kiran
In this study, Lactobacillus plantarum F-10, a promising probiotic strain isolated from fecal microbiota of healthy breastfed infant, was assessed as a bio-control strategy for wound infections. Pseudomonas aeruginosa PAO1/ATCC 27853, methicillin-resistant Staphylococcus aureus ATCC 43300, and their hospital-derived strains isolated from skin chronic wound samples were used as important skin pathogens. The cell-free extract (CFE) of the strain F-10 was shown to inhibit the growth of all pathogens tested, while no inhibition was observed when CFE was neutralized...
December 6, 2018: Probiotics and Antimicrobial Proteins
https://www.readbyqxmd.com/read/30497871/biofilms-in-chronic-wounds-pathogenesis-and-diagnosis
#3
REVIEW
Yuan-Kun Wu, Nai-Chen Cheng, Chao-Min Cheng
Chronic non-healing wounds have become a major worldwide healthcare burden. The impact of biofilms on chronic wound infection is well established. Despite increasing understanding of the underlying mechanism of biofilm formation in chronic wounds, current strategies for biofilm diagnosis in chronic wounds are still far from ideal. In this review, we briefly summarize the mechanism of biofilm formation and focus on current diagnostic approaches of chronic wound biofilms based on morphology, microbiology, and molecular assays...
November 26, 2018: Trends in Biotechnology
https://www.readbyqxmd.com/read/30487573/disease-associated-genotypes-of-the-commensal-skin-bacterium-staphylococcus-epidermidis
#4
Guillaume Méric, Leonardos Mageiros, Johan Pensar, Maisem Laabei, Koji Yahara, Ben Pascoe, Nattinee Kittiwan, Phacharaporn Tadee, Virginia Post, Sarah Lamble, Rory Bowden, James E Bray, Mario Morgenstern, Keith A Jolley, Martin C J Maiden, Edward J Feil, Xavier Didelot, Maria Miragaia, Herminia de Lencastre, T Fintan Moriarty, Holger Rohde, Ruth Massey, Dietrich Mack, Jukka Corander, Samuel K Sheppard
Some of the most common infectious diseases are caused by bacteria that naturally colonise humans asymptomatically. Combating these opportunistic pathogens requires an understanding of the traits that differentiate infecting strains from harmless relatives. Staphylococcus epidermidis is carried asymptomatically on the skin and mucous membranes of virtually all humans but is a major cause of nosocomial infection associated with invasive procedures. Here we address the underlying evolutionary mechanisms of opportunistic pathogenicity by combining pangenome-wide association studies and laboratory microbiology to compare S...
November 28, 2018: Nature Communications
https://www.readbyqxmd.com/read/30472614/catalytic-antibacterial-and-antibiofilm-efficacy-of-biosynthesised-silver-nanoparticles-using-prosopis-juliflora-leaf-extract-along-with-their-wound-healing-potential
#5
Geeta Arya, R Mankamna Kumari, Nikita Sharma, Nidhi Gupta, Ajeet Kumar, Sreemoyee Chatterjee, Surendra Nimesh
The present study focuses on the catalytic, antibacterial and antibiofilm efficacy of silver nanoparticles (AgNPs) in an easy, rapid and eco-friendly pathway. Herein, we have synthesised AgNPs using an aqueous extract of P. juliflora leaf. The bioactive compounds present in the extract are responsible for the reduction of Ag+ to Ag0 . The particle synthesis was first observed by visual color change and then characterized using UV-visible spectroscopy to confirm the formation of AgNPs. The synthesis conditions were then optimised using critical parameters such as reaction time, AgNO3 concentration, extract to AgNO3 ratio and temperature of the reaction...
November 15, 2018: Journal of Photochemistry and Photobiology. B, Biology
https://www.readbyqxmd.com/read/30465823/antimicrobial-synergy-of-monolaurin-lipid-nanocapsules-with-adsorbed-antimicrobial-peptides-against-staphylococcus-aureus-biofilms-in-vitro-is-absent-in-vivo
#6
René T Rozenbaum, Linzhu Su, Anita Umerska, Matthieu Eveillard, Joakim Håkansson, Margit Mahlapuu, Fan Huang, Jianfeng Liu, Zhenkun Zhang, Linqi Shi, Henny C van der Mei, Henk J Busscher, Prashant K Sharma
Bacterial infections are mostly due to bacteria in their biofilm-mode of growth, while penetrability of antimicrobials into infectious biofilms and increasing antibiotic resistance hamper infection treatment. In-vitro, monolaurin lipid nanocapsules (ML-LNCs) carrying adsorbed antimicrobial peptides (AMPs) displayed synergistic efficacy against planktonic Staphylococcus aureus, but it has not been demonstrated, neither in-vitro nor in-vivo, that such ML-LNCs penetrate into infectious S. aureus biofilms and maintain synergy with AMPs...
November 19, 2018: Journal of Controlled Release: Official Journal of the Controlled Release Society
https://www.readbyqxmd.com/read/30423761/fabrication-and-in-vitro-behavior-of-dual-function-chitosan-silver-nanocomposites-for-potential-wound-dressing-applications
#7
A Hernández-Rangel, P Silva-Bermudez, B L España-Sánchez, E Luna-Hernández, A Almaguer-Flores, C Ibarra, V I Garcia-Perez, C Velasquillo, G Luna-Barcenas
We report the synthesis and in vitro evaluation of dual-function chitosan-silver nanoparticles (CTS-AgNPs) films with potential applications as wound dressings. We attempted to formulate nanocomposite films with appropriate AgNPs concentrations to simultaneously display antibacterial activity and suitability for cell culture. Nanocomposites were obtained by CTS-mediated in situ chemical reduction of AgNO3 . Circular-shape AgNPs (sizes ca. 7-50 nm) well distributed within the CTS matrices were obtained in concentrations from 0...
January 1, 2019: Materials Science & Engineering. C, Materials for Biological Applications
https://www.readbyqxmd.com/read/30418057/a-systematic-review-of-local-antibiotic-devices-used-to-improve-wound-healing-following-the-surgical-management-of-foot-infections-in-diabetics
#8
REVIEW
B A Marson, S R Deshmukh, D J C Grindlay, B J Ollivere, B E Scammell
AIMS: Local antibiotics are used in the surgical management of foot infection in diabetic patients. This systematic review analyzes the available evidence of the use of local antibiotic delivery systems as an adjunct to surgery. MATERIALS AND METHODS: Databases were searched to identify eligible studies and 13 were identified for inclusion. RESULTS: Overall, the quality of the studies was poor. A single trial suggested that wound healing is quicker when a gentamicin-impregnated collagen sponge was implanted at time of surgery, with no difference in length of stay or rate of amputation...
November 2018: Bone & Joint Journal
https://www.readbyqxmd.com/read/30405717/antifungal-susceptibility-and-biofilm-production-of-candida-spp-isolated-from-clinical-samples
#9
Munmun B Marak, Biranthabail Dhanashree
Objective: The study aims to speciate clinical Candida isolates and detect their biofilm-forming ability and antifungal resistance. Methods: All the Candida spp. isolated from different clinical samples like pus, urine, blood, and body fluid were included in the study. Biofilm production was tested by the microtiter plate method. Antifungal susceptibility was studied by the disk diffusion method. Patient's demographic details such as age, sex, and clinical information were collected...
2018: International Journal of Microbiology
https://www.readbyqxmd.com/read/30401769/matrix-polysaccharides-and-siad-diguanylate-cyclase-alter-community-structure-and-competitiveness-of-pseudomonas-aeruginosa-during-dual-species-biofilm-development-with-staphylococcus-aureus
#10
Su Chuen Chew, Joey Kuok Hoong Yam, Artur Matysik, Zi Jing Seng, Janosch Klebensberger, Michael Givskov, Patrick Doyle, Scott A Rice, Liang Yang, Staffan Kjelleberg
Mixed-species biofilms display a number of emergent properties, including enhanced antimicrobial tolerance and communal metabolism. These properties may depend on interspecies relationships and the structure of the biofilm. However, the contribution of specific matrix components to emergent properties of mixed-species biofilms remains poorly understood. Using a dual-species biofilm community formed by the opportunistic pathogens Pseudomonas aeruginosa and Staphylococcus aureus , we found that whilst neither Pel nor Psl polysaccharides, produced by P...
November 6, 2018: MBio
https://www.readbyqxmd.com/read/30398942/skin-integrity-and-infection-prevention-las-vegas-the-science-of-biofilm-a-multifaceted-challenge-to-healing
#11
(no author information available yet)
At the 4th International Skin Integrity and Infection Prevention conference, hosted by the Journal of Wound Care and the University of Huddersfield, in Las Vegas, one of the main themes was the control and resolution of biofilm. A series of reports will describe the key points of four sponsored symposia at the event. The first of these concentrates on the role of biofilm in chronic wounds and new therapies to aid the healing of these wounds by disrupting biofilm.
November 2, 2018: Journal of Wound Care
https://www.readbyqxmd.com/read/30398940/skin-integrity-and-infection-prevention-las-vegas-don-t-bust-on-biofilm-bet-on-dhacm
#12
(no author information available yet)
The 4th International Skin Integrity and Infection Prevention conference, hosted by the Journal of Wound Care and the University of Huddersfield, was held earlier this year in Las Vegas. A key theme was the impact of biofilm on wound healing. In the second of our sponsored symposia reports, the manner in which delayed healing can be reversed through effective biofilm management, and the introduction of regulatory proteins found in dehydrated human amnion chorion membrane allograft were explained.
November 2, 2018: Journal of Wound Care
https://www.readbyqxmd.com/read/30388366/a-dual-function-antibiotic-transporter-conjugate-exhibits-superior-activity-in-sterilizing-mrsa-biofilms-and-killing-persister-cells
#13
Alexandra Antonoplis, Xiaoyu Zang, Melanie A Huttner, Kelvin K L Chong, Yu B Lee, Julia Y Co, Manuel R Amieva, Kimberly A Kline, Paul A Wender, Lynette Cegelski
New strategies are urgently needed to target MRSA, a major global health problem and the leading cause of mortality from antibiotic-resistant infections in many countries. Here, we report a general approach to this problem exemplified by the design and synthesis of a vancomycin-d-octaarginine conjugate (V-r8) and investigation of its efficacy in addressing antibiotic-insensitive bacterial populations. V-r8 eradicated MRSA biofilm and persister cells in vitro, outperforming vancomycin by orders of magnitude...
November 28, 2018: Journal of the American Chemical Society
https://www.readbyqxmd.com/read/30384254/biosurfactant-coated-silver-and-iron-oxide-nanoparticles-with-enhanced-anti-biofilm-and-anti-adhesive-properties
#14
Hafiza Faiza Khalid, Bushra Tehseen, Yasra Sarwar, Syed Zajif Hussain, Waheed S Khan, Zulfiqar Ali Raza, Sadia Zafar Bajwa, Antonios G Kanaras, Irshad Hussain, Asma Rehman
Pseudomonas aeruginosa and Staphylococcus aureus are among the hazardous biofilm forming bacteria ubiquitous in industrial/clinical wastes. Serious efforts are required to develop effective strategies to control surface-growing antibiotic resistant pathogenic bacterial communities which they are emerging as a global health issue. Blocking hazardous biofilms would be a useful aspect of biosurfactant coated nanoparticles (NPs). In this regard, we report a facile method for the synthesis of rhamnolipid (RL) coated silver (Ag) and iron oxide (Fe3 O4 ) NPs and propose the mechanism of their synergistic antibacterial and anti-adhesive properties against biofilms formed by P...
October 19, 2018: Journal of Hazardous Materials
https://www.readbyqxmd.com/read/30374716/lipopeptides-from-bacillus-amyloliquefaciens-strain-32a-as-promising-biocontrol-compounds-against-the-plant-pathogen-agrobacterium-tumefaciens
#15
Dorra Ben Abdallah, Slim Tounsi, Houda Gharsallah, Adnane Hammami, Olfa Frikha-Gargouri
Despite the potential biological importance of lipopeptides from Bacillus amyloliquefaciens as antimicrobial compounds, their effects on Agrobacterium tumefaciens biofilms have not been previously studied. These latter are important virulence factors for the development and re-occurrence of crown gall disease. As part of the development of a new biopesticide acting as anti-biofilm and biocontrol agent, we investigated for the first time the ability of a mixture of lipopeptides produced by B. amyloliquefaciens strain 32a to inhibit the tumor formation on plants and to reduce the formation of biofilms by the phytopathogenic A...
October 29, 2018: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/30374416/advanced-smart-biomaterials-and-constructs-for-hard-tissue-engineering-and-regeneration
#16
Ke Zhang, Suping Wang, Chenchen Zhou, Lei Cheng, Xianling Gao, Xianju Xie, Jirun Sun, Haohao Wang, Michael D Weir, Mark A Reynolds, Ning Zhang, Yuxing Bai, Hockin H K Xu
Hard tissue repair and regeneration cost hundreds of billions of dollars annually worldwide, and the need has substantially increased as the population has aged. Hard tissues include bone and tooth structures that contain calcium phosphate minerals. Smart biomaterial-based tissue engineering and regenerative medicine methods have the exciting potential to meet this urgent need. Smart biomaterials and constructs refer to biomaterials and constructs that possess instructive/inductive or triggering/stimulating effects on cells and tissues by engineering the material's responsiveness to internal or external stimuli or have intelligently tailored properties and functions that can promote tissue repair and regeneration...
2018: Bone Research
https://www.readbyqxmd.com/read/30351262/human-adipose-tissue-derived-mesenchymal-stem-stromal-cells-adhere-to-and-inhibit-the-growth-of-staphylococcus-aureus-and-pseudomonas-aeruginosa
#17
Chelsea Rheannon Wood, Douaa Al Dhahri, Ibtesam Al Delfi, Neil Anthony Pickles, Rachel L Sammons, Tony Worthington, Karina Theresa Wright, William Eustace Basil Johnson
We have cultured and phenotyped human adipose tissue-derived mesenchymal stem/stromal cells (AT MSCs) and inoculated these cultures with bacteria common to infected skin wounds, i.e. Staphylococcus aureus and Pseudomonas aeruginosa. Cell interactions were examined by scanning electron microscopy (SEM), whilst bacterial growth was measured by colony forming unit (c.f.u.) and biofilm assays. AT MSCs appeared to attach to the bacteria and to engulf S. aureus. Significantly fewer bacterial c.f.u. were present in AT MSC : bacterial co-cultures compared with bacteria cultured alone...
October 23, 2018: Journal of Medical Microbiology
https://www.readbyqxmd.com/read/30345928/anti-pathogenic-efficacy-of-a-polyherbal-wound-care-formulation-herboheal-against-staphylococcus-aureus-and-identifying-its-molecular-targets
#18
Pooja Patel, Chinmayi Joshi, Vijay Kothari
BACKGROUND: A polyherbal formulation (Herboheal) traditionally indicated for wound-care was investigated for its anti-virulence potential against the notorious pathogen Staphylococcus aureus. OBJECTIVE: This study aimed at evaluating anti-virulence potential of Herboheal formulation against S. aureus in vitro as well as in vivo, followed by studying its effect on target bacterium's gene expression at the whole transcriptome level. METHOD: In vitro efficacy of the test formulation was evaluated using broth dilution assay, whereas in vivo efficacy was assayed employing the nematode Caenorhabditis elegans as the model host...
October 22, 2018: Infectious Disorders Drug Targets
https://www.readbyqxmd.com/read/30344513/bactericidal-property-of-oregano-oil-against-multidrug-resistant-clinical-isolates
#19
Min Lu, Tianhong Dai, Clinton K Murray, Mei X Wu
Development of non-antibiotic alternatives to treat infections caused by multidrug-resistant (MDR) microbes represents one of the top priorities in healthcare and community settings, especially in the care of combat trauma-associated wound infections. Here, we investigate efficacy of oregano oil against pathogenic bacteria including MDR isolates from the combat casualties in vitro and in a mouse burn model. Oregano oil showed a significant anti-bacterial activity against 11 MDR clinical isolates including four Acinetobacter baumannii , three Pseudomonas aeruginosa , and four methicillin-resistant Staphylococcus aureus (MRSA) obtained from combat casualties and two luminescent strains of PA01 and MRSA USA300, with a MIC ranging from 0...
2018: Frontiers in Microbiology
https://www.readbyqxmd.com/read/30342803/medical-therapy-in-equine-wound-management
#20
REVIEW
R Reid Hanson
Suitable use of prophylactic antimicrobial drugs for wounds depends on the accurate selection of appropriate antibiotics, dosing regimen, and duration of use. Regional intravenous delivery and intraosseous infusion of antibiotics are pivotal to a successful outcome for deep-rooted infections, inadequately perfused tissue, and infected wounds containing biofilm. Antibiotic-impregnated polymethylmethacrylate beads are predominantly helpful for wounds that have a poor blood supply and for those containing surgical implants that must remain in place...
December 2018: Veterinary Clinics of North America. Equine Practice
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