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Clot composition

Vail Natale Júnior, Francisley Ávila Souza, Eduardo Vedovatto, Renato Sussumu Nishioka, Pier Paolo Poli, Paulo Sérgio Perri de Carvalho
The purpose of this study was to evaluate the preservation of alveolar dimensions in human fresh extraction sockets filled with a composite bovine bone graft by means of design of single-blind randomized clinical trial. Forty participants had monoradicular teeth extracted (one teeth in each participant), and after were randomly divided into 2 groups: individuals whose fresh sockets were filled with the composite heterologous bone graft (Biomaterial Group), or with blood clot (Control Group). After extraction, the fresh sockets were measured at their greatest mesiodistal (MD) and bucco-lingual/palatal (BL/P) distance...
November 2018: Brazilian Dental Journal
Seán Fitzgerald, Oana M Mereuta, Karen M Doyle, Daying Dai, Ramanathan Kadirvel, David F Kallmes, Waleed Brinjikji
Mechanical thrombectomy has become the stand of care for patients with large vessel occlusions, yet major improvements in thrombectomy speed, efficacy, and completeness can still be achieved. High rates of clot fragmentation and failure to remove the clot resulting in poor neurological outcomes suggest that in order to further advance the field of stroke intervention; we must turn our attention towards understanding the science of clot. Accurately identifying the composition of the occlusive clot prior to intervention could significantly influence the success of the revascularization strategy used to treat them...
December 5, 2018: Journal of Neurosurgical Sciences
Yu Zhong, Xingtao Chen, Haitao Peng, Zhengwen Ding, Yonggang Yan
The novel Ca-zeolite/poly(amino acid) (CaY/PAA) composites for bone substitute applications with hemostatic activity were prepared using the in situ melting polymerization method. In this study, Ca-zeolite (CaY) loaded with Ca2+ was obtained from Y-type zeolite (NaY) by ion-exchange method. The properties of the CaY/PAA composites and PAA, including composition, structure, compressive strength, in vitro degradability in phosphate-buffered solution (PBS), bioactivity, cytocompatibility and in vitro coagulation tests were characterized and investigated...
November 24, 2018: Journal of Biomaterials Science. Polymer Edition
Aneta Stachowicz, Michal Zabczyk, Joanna Natorska, Maciej Suski, Rafał Olszanecki, Ryszard Korbut, Jacek R Wiśniewski, Anetta Undas
The prothrombotic fibrin clot phenotype has been reported in patients with thrombotic antiphospholipid syndrome (APS) and venous thromboembolism (VTE). Protein composition of plasma fibrin clots in APS has not been studied. We evaluated 23 patients with thrombotic APS, 19 with VTE alone, and 20 well-matched controls. A proteomic analysis of fibrin clots generated from citrated plasma was based on liquid chromatography-mass spectrometry. Plasma levels of thrombospondin-1 (TSP1), apolipoprotein(a), A-I, and B-100, complement components (C)3a, C5b-C9, histidine-rich glycoprotein (HRG), and prothrombin were evaluated using immunoenzymatic tests...
November 23, 2018: Scientific Reports
Grzegorz Gajos, Aleksander Siniarski, Joanna Natorska, Michał Ząbczyk, Jakub Siudut, Krzysztof Piotr Malinowski, Renata Gołębiowska-Wiatrak, Paweł Rostoff, Anetta Undas
BACKGROUND: Little is known about factors that affect the composition of contracted blood clots in specific diseases. We investigated the content of polyhedral erythrocytes (polyhedrocytes) formed in blood clots and its determinants in type 2 diabetes (T2D) patients. METHODS: In 97 patients with long-standing T2D [median HbA1c , 6.4% (interquartile range 5.9-7.8)], we measured in vitro the composition of blood clots, including a clot area covered by polyhedrocytes using scanning electron microscopy and the erythrocyte compression index (ECI), defined as a ratio of the mean polyhedrocyte area to the mean native erythrocyte area...
November 22, 2018: Cardiovascular Diabetology
Carsten Horn, Claude Négrier, Uwe Kalina, Wilfried Seifert, Kenneth D Friedman
BACKGROUND: Measuring factor IX activity (FIX:C) with one-stage clotting (OSC) assays, based on the activated partial thromboplastin time (aPTT), is the current mainstay of diagnostic techniques for hemophilia B. Assessing the performance of new recombinant FIX (rFIX) products in OSC assays is essential as aPTT reagents from different manufacturers yield different potency estimates for rFIX. OBJECTIVES: To evaluate the extent to which choice of reagent composition influences rFIX potency measurements of recombinant FIX-albumin fusion protein (rIX-FP, IDELVION® ) activity in OSC assays...
November 12, 2018: Journal of Thrombosis and Haemostasis: JTH
Cui Yan Chao, Mohan Prasath Mani, Saravana Kumar Jaganathan
Essential oils play an important role in reducing the pain and inflammation caused by bone fracture.In this study, a scaffold was electrospun based on polyurethane (PU), grape seed oil, honey and propolis for bone tissue-engineering applications. The fiber diameter of the electrospun PU/grape seed oil scaffold and PU/grape seed oil/honey/propolis scaffold were observed to be reduced compared to the pristine PU control. FTIR analysis revealed the existence of grape seed oil, honey and propolis in PU identified by CH band peak shift and also hydrogen bond formation...
2018: PloS One
L I Malinova, N V Furman, P V Dolotovskaya, L A Chernousova, T P Denisova
PURPOSE: to assess the possibility of the use of ADP induced blood-clotting time measurement in clinical practice prognostication of the course of acute coronary syndrome (ACS) and assessment of effectiveness of antiplatelet therapy (APT). MATERIALS AND METHODS: We enrolled in the study 163 male patients admitted to the coronary unit for acute coronary syndrome (ACS) and 38 male practically healthy volunteers (PHV). ADP induced blood-clotting time (ADP BCT) was measured as time (sec) between addition of ADP (10 μcmol) to recalcificated sample of citrate blood and clot formation...
June 2018: Kardiologiia
Kathleen E Brummel-Ziedins, Matthew Gissel, Jacqueline Neuhaus, Álvaro H Borges, David R Chadwick, Sean Emery, James D Neaton, Russell P Tracy, Jason V Baker
Background: Effective HIV treatment with antiretroviral therapy has prolonged survival and shifted causes of death to non-AIDS illnesses such as cardiovascular disease. We have shown that inflammation and HIV viral load associate with pro- and anticoagulant factor imbalances resulting in increased thrombin generation when mathematically modeled. We explore the hypothesis that factor compositional imbalance, corresponding to increased in silico thrombin generation, predicts mortality among HIV+ persons...
October 2018: Research and Practice in Thrombosis and Haemostasis
Patrick A Brouwer, Waleed Brinjikji, Simon F De Meyer
Cerebral ischemic stroke treatment may change significantly now that clots are actually physically removed from the patient using thrombectomy. This allows for an analysis of the content of the clots as well as the correlation of the imaging findings and the clot behavior and morphology. This article illustrates how the interaction of different clots varies in the clinical setting and how analysis of clot composition, as well as the search for new pharmacologic targets, can lead to a better understanding of the pathophysiology and therapy resistance, in turn providing possibilities for a better approach in the treatment...
November 2018: Neuroimaging Clinics of North America
Artur Y Prilepskii, Anna F Fakhardo, Andrey S Drozdov, Vasiliy V Vinogradov, Ivan P Dudanov, Alexander A Shtil, Petr P Bel'tyukov, Alexey M Shibeko, Ekaterina M Koltsova, Dmitry Y Nechipurenko, Vladimir V Vinogradov
Mortality and disabilities as outcomes of cardiovascular diseases are primarily related to blood clotting. Optimization of thrombolytic drugs is aimed at the prevention of side effects (in particular, bleeding) associated with a disbalance between coagulation and anticoagulation caused by systemically administered agents. Minimally invasive and efficient approaches to deliver the thrombolytic agent to the site of clot formation are needed. Herein, we report a novel nanocomposite prepared by heparin-mediated cross-linking of urokinase with magnetite nanoparticles (MNPs@uPA)...
October 31, 2018: ACS Applied Materials & Interfaces
Moslem Afsharnezhad, S Shirin Shahangian, Reyhaneh Sariri
Due to the need for calf rennet alternatives, many attempts have been made to find new proteases. A novel cysteine protease with milk-clotting activity was purified from Ficus johannis by cation exchange chromatography. The protease was stable in various pH (3.0-10.5) with the optimum at 6.5 and showed its maximum activity at 60 °C. The Km and Vmax values of the enzyme were obtained to be 0.604 mg/ml and 0.0273 μmol Tyr/min, respectively. The purified protease exhibited considerable activity towards κ-casein in comparison to α-casein and β-casein...
January 2019: International Journal of Biological Macromolecules
Karla P Mercado-Shekhar, Robert T Kleven, Hermes Aponte Rivera, Ryden Lewis, Kunal B Karani, Hendrik J Vos, Todd A Abruzzo, Kevin J Haworth, Christy K Holland
The lytic recombinant tissue plasminogen activator (rt-PA) is the only drug approved by the Food and Drug Administration for treating ischemic stroke. Less than 40% of patients with large vessel occlusions who are treated with rt-PA have improved blood flow. However, up to 6% of all patients receiving rt-PA develop intracerebral hemorrhage. Predicting the efficacy of rt-PA treatment a priori could help guide therapeutic decision making, such that rt-PA is administered only to those individuals who would benefit from this treatment...
December 2018: Ultrasound in Medicine & Biology
Anne Preut, Megan Laughlin, Hanna Jensen, Jamie Hestekin, Morten Jensen
BACKGROUND: Unsuccessful recanalization attempts in stroke patients have been associated with fibrin-rich thromboemboli linking retrieval mechanism performance and clot composition. To continue development of stroke retrieval mechanisms, the material properties of cerebral thromboemboli must be replicated; however, current methods for emboli analog formation lack quantitative measurements for both material stiffness and composition of cerebral thromboemboli. This study investigates emboli analog (EA) formation to mimic the material stiffness and composition of cerebral thromboemboli to develop new retrieval mechanisms...
September 11, 2018: Journal of Biomechanics
Jonathan Vigne, James Thackeray, Jeroen Essers, Marcus Makowski, Zoreh Varasteh, Adelina Curaj, Angelos Karlas, Emmanuel Canet-Soulas, Willem Mulder, Fabian Kiessling, Michael Schäfers, René Botnar, Moritz Wildgruber, Fabien Hyafil
Atherosclerotic plaques can remain quiescent for years, but become life threatening upon rupture or disruption, initiating clot formation in the vessel lumen and causing acute myocardial infarction and ischemic stroke. Whether and how a plaque ruptures is determined by its macroscopic structure and microscopic composition. Rupture-prone plaques usually consist of a thin fibrous cap with few smooth muscle cells, a large lipid core, a dense infiltrate of inflammatory cells, and neovessels. Such lesions, termed high-risk plaques, can remain asymptomatic until the thrombotic event...
December 2018: Molecular Imaging and Biology: MIB: the Official Publication of the Academy of Molecular Imaging
Yuqing Cai, Peng Chen, Cuiyun Wu, Jinyou Duan, Hongjin Bai
Two polysaccharides JCS-1 and JCS-2 were successfully purified by DEAE-52 Cellulose chromatography from Zizyphus jujuba cv. Jinchangzao. Their structures were analyzed by high performance liquid chromatography (HPLC), high-performance gel permeation chromatography (HPGPC), nuclear magnetic resonance (NMR), and fourier transform infrared (FT-IR) spectroscopy. Further, eight sulfated derivatives are prepared by chlorosulfonic acid-pyridine (CSA-Pyr). Structural analysis revealed that both JCS-1 and JCS-2 were size uniform polysaccharides with the average molecular weight (Mw) of 71...
December 2018: International Journal of Biological Macromolecules
Mohsen Ramazani, Saeed Asgary
This case report describes miniature pulpotomy (MP) with calcium-enriched mixture (CEM) cement, 1 week after carious pulpal exposure of a symptomatic mature molar. A 24-year-old woman was referred with complaining of severe lingering pain on the second upper left molar; a dental history revealed that the tooth had been prepared 1 week ago, but on pulp exposure, her dentist just dressed the cavity. After anesthesia/isolation in the same session, the temporary restoration was removed, the previously pulpal exposure was observed, and MP was carried out...
July 2018: Dental Research Journal
Nicoletta Riva, Kevin Vella, Kieron Hickey, Lorenza Bertù, Daniel Zammit, Silvana Spiteri, Steve Kitchen, Michael Makris, Walter Ageno, Alex Gatt
BACKGROUND: The diagnostic algorithm for venous thromboembolism (VTE) currently involves a composite of pre-test probability, D-dimer and imaging. Other laboratory tests, however, may assist in the identification of patients with VTE. AIM: To assess the accuracy of different coagulation tests (D-dimer, thrombin generation, phospholipid-dependent (PPL) clotting time, soluble P-selectin (sP-selectin)) as biomarkers of acute VTE. METHODS: Random samples arriving at the Coagulation Laboratory at Mater Dei Hospital (Msida, Malta) from the Accident and Emergency Department with a request for D-dimer measurement were collected between August 2015 and February 2016...
November 2018: Journal of Clinical Pathology
Saravana Kumar Jaganathan, Mohan Prasath Mani
In this study, a wound dressing based on polyurethane (PU) blended with copper sulphate nanofibers was developed using an electrospinning technique. The prepared PU and PU nanocomposites showed smooth fibers without any bead defects. The prepared nanocomposites showed smaller fiber (663 ± 156.30 nm) and pore (888 ± 70.93 nm) diameter compared to the pristine PU (fiber diameter 1159 ± 147.48 nm and pore diameter 1087 ± 62.51 nm). The interaction of PU with copper sulphate was evident in the infrared spectrum through hydrogen-bond formation...
August 2018: 3 Biotech
Marinus Fw Te Pas, Sietse-Jan Koopmans, Leo Kruijt, Sjef Boeren, Mari A Smits
Recognition of the preclinical stages of metabolic diseases such as diabetes helps to prevent full development of the disease. In our research, we alter the diet composition of pigs to create a model of human metabolic disease. The objective of the current study was to identify plasma proteins and biologic mechanisms that differed in expression between pigs fed a 'cafeteria diet' (considered unhealthy; high in saturated fats) and those fed a 'Mediterranean diet' (considered healthy; high in unsaturated fats)...
August 1, 2018: Comparative Medicine
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