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https://www.readbyqxmd.com/read/28231235/near-real-time-surveillance-of-u-s-norovirus-outbreaks-by-the-norovirus-sentinel-testing-and-tracking-network-united-states-august-2009-july-2015
#1
Minesh P Shah, Mary E Wikswo, Leslie Barclay, Anita Kambhampati, Kayoko Shioda, Umesh D Parashar, Jan Vinjé, Aron J Hall
Norovirus is the leading cause of endemic and epidemic acute gastroenteritis in the United States (1). New variant strains of norovirus GII.4 emerge every 2-4 years (2-4) and are often associated with increased disease and health care visits (5-7). Since 2009, CDC has obtained epidemiologic data on norovirus outbreaks from state health departments through the National Outbreak Reporting System (NORS) (8) and laboratory data through CaliciNet (9). NORS is a web-based platform for reporting waterborne, foodborne, and enteric disease outbreaks of all etiologies, including norovirus, to CDC...
February 24, 2017: MMWR. Morbidity and Mortality Weekly Report
https://www.readbyqxmd.com/read/28224385/evaluation-of-real-time-rt-pcr-assays-for-detection-and-quantification-of-norovirus-genogroups-i-and-ii
#2
Kitwadee Rupprom, Porntip Chavalitshewinkoon-Petmitr, Pornphan Diraphat, Leera Kittigul
Noroviruses are the leading cause of acute gastroenteritis in humans. Real-time reverse transcription-polymerase chain reaction (real-time RT-PCR) is a promising molecular method for the detection of noroviruses. In this study, the performance of three TaqMan real-time RT-PCR assays was assessed, which were one commercially available real-time RT-PCR kit (assay A: Norovirus Real Time RT-PCR kit) and two in-house real-time RT-PCR assays (assay B: LightCycler RNA Master Hybprobe and assay C: RealTime ready RNA Virus Master)...
February 20, 2017: Virologica Sinica
https://www.readbyqxmd.com/read/28221985/role-of-food-handlers-in-norovirus-outbreaks-in-london-and-south-east-england-2013-to-2015
#3
C Rumble, S Addiman, S Balasegaram, K Chima, D Ready, J Heard, E Alexander
Outbreaks caused by norovirus infection are common and occur throughout the year. Outbreaks can be related to food outlets either through a contaminated food source or an infected food handler. Both asymptomatic and symptomatic food handlers are potentially implicated in outbreaks, but evidence of transmission is limited. To understand potential food handler transmission in outbreak scenarios, epidemiological and microbiological data on possible and confirmed norovirus outbreaks reported in London and South East England in a 2-year period were reviewed...
February 2017: Journal of Food Protection
https://www.readbyqxmd.com/read/28221974/evaluation-of-a-surface-sampling-method-for-recovery-of-human-noroviruses-prior-to-detection-using-reverse-transcription-quantitative-pcr
#4
Grace Tung-Thompson, Blanca I Escudero-Abarca, Janie Outlaw, Arnaud Ganee, Sylvanie Cassard, Claude Mabilat, Lee-Ann Jaykus
Human noroviruses are the most common cause of acute viral gastroenteritis, and the environmental persistence of these viruses contributes to their transmissibility. Environmental sampling is thus an important tool for investigating norovirus outbreaks and for assessing the effectiveness of cleaning and decontamination regimens. The purpose of this study was to evaluate a sampling material (wipes) for their efficacy at recovering human norovirus from hard surfaces and foods. Dilutions of a human norovirus GII...
February 2017: Journal of Food Protection
https://www.readbyqxmd.com/read/28221948/a-state-by-state-assessment-of-food-service-regulations-for-prevention-of-norovirus-outbreaks
#5
Anita Kambhampati, Kayoko Shioda, L Hannah Gould, Donald Sharp, Laura G Brown, Umesh D Parashar, Aron J Hall
Noroviruses are the leading cause of foodborne disease in the United States. Foodborne transmission of norovirus is often associated with contamination of food during preparation by an infected food worker. The U.S. Food and Drug Administration's Food Code provides model food safety regulations for preventing transmission of foodborne disease in restaurants; however, adoption of specific provisions is at the discretion of state and local governments. We analyzed the food service regulations of all 50 states and the District of Columbia (i...
September 2016: Journal of Food Protection
https://www.readbyqxmd.com/read/28221934/effect-of-process-temperature-on-virus-inactivation-during-high-hydrostatic-pressure-processing-of-contaminated-fruit-puree-and-juice
#6
Hao Pan, Matthew Buenconsejo, Karl F Reineke, Y Carol Shieh
High pressure processing (HPP) can inactivate pathogens and retain fruit qualities. Elevated HPP pressure or time increases virus inactivation, but the effect of temperature is not consistently observed for norovirus and hepatitis A virus. In the present study, the effectiveness of HPP holding temperatures (<40°C) and pressures were evaluated for inactivating surrogates (murine norovirus [MNV] and MS2 coliphage) in pomegranate and strawberry juices and strawberry puree using a 24-liter HPP system. The holding temperature was established by setting the HPP initial temperature via pretrials...
September 2016: Journal of Food Protection
https://www.readbyqxmd.com/read/28221908/content-analysis-of-vomit-and-diarrhea-cleanup-procedures-to-prevent-norovirus-infections-in-retail-and-food-service-operations
#7
Morgan G Chao, Anne-Julie Dubé, Cortney M Leone, Christina M Moore, Angela M Fraser
Human noroviruses are the leading cause of foodborne disease in the United States, sickening 19 to 21 million Americans each year. Vomit and diarrhea are both highly concentrated sources of norovirus particles. For this reason, establishing appropriate cleanup procedures for these two substances is critical. Food service establishments in states that have adopted the 2009 or 2013 U.S. Food and Drug Administration Food Code are required to have a program detailing specific cleanup procedures. The aim of our study was to determine the alignment of existing vomit and diarrhea cleanup procedures with the 11 elements recommended in Annex 3 of the 2011 Supplement to the 2009 Food Code and to determine their readability and clarity of presentation...
November 2016: Journal of Food Protection
https://www.readbyqxmd.com/read/28221842/consumer-response-to-gastrointestinal-illness-perceived-to-originate-from-food-service-facilities
#8
Erin S Garnett, Stephanie R Gretsch, Clair Null, Christine L Moe
Consumer responses to food product recalls have been documented, but there is little information on how consumers respond to illnesses or outbreaks associated with food service facilities. This study uses an on-line survey of 885 adults conducted in 2012 to determine how respondents changed their dining behavior following personal experiences with and secondhand reports of gastrointestinal illness believed to be associated with food service facilities. In response to personally experiencing gastrointestinal illness that they attributed to a food service facility, 90% of survey participants reported that they avoided the implicated facility for a time following the incident; almost one-half decided to never return to the facility they believed had made them ill...
October 2016: Journal of Food Protection
https://www.readbyqxmd.com/read/28219831/expression-and-characterization-of-the-major-capsid-protein-derived-from-a-gii-6-norovirus-strain-isolated-in-china
#9
Yuqi Huo, Lijun Zheng, Xuhui Chen, Lili Ge, Yumei Wang
The objective of this study was to express and characterize the major capsid protein (VP1) of a GII.6 Norovirus (NoV)strain isolated in China. The newly identified GII.6 NoV strain was isolated from a five-year old boy presenting acute gastroenteritis. The genome of the GII.6 strain was 7550 nucleotides in length, excluding the poly-adenylation tail. Multiple sequence alignment and phylogenetic analysis based on deduced VP1 amino acid sequences from different genotypes indicated close relationship between GII...
February 20, 2017: Microbial Pathogenesis
https://www.readbyqxmd.com/read/28213546/environmental-surveillance-of-norovirus-genogroups-i-and-ii-for-sensitive-detection-of-epidemic-variants
#10
Shinobu Kazama, Takayuki Miura, Yoshifumi Masago, Yoshimitsu Konta, Kentaro Tohma, Takafumi Manaka, Xiaofang Liu, Daisuke Nakayama, Takashi Tanno, Mayuko Saito, Hitoshi Oshitani, Tatsuo Omura
Sewage samples have been investigated to study the level of noroviruses concentration in sewage or the genotypes of noroviruses circulating in human populations. However, the statistical relationship between the concentration of the virus and the number of infected individuals, and the clinical importance of genotypes or strains detected in sewage are unclear. In this study, we carried out both environmental and clinical surveillance of noroviruses for three years: 2013-2016. We performed cross-correlation analysis between the concentrations of norovirus GI or GII in sewage samples collected weekly and the number of gastroenteritis cases...
February 17, 2017: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/28212439/correction-type-i-interferon-receptor-deficiency-in-dendritic-cells-facilitates-systemic-murine-norovirus-persistence-despite-enhanced-adaptive-immunity
#11
Timothy J Nice, Lisa C Osborne, Vesselin T Tomov, David Artis, E John Wherry, Herbert W Virgin
[This corrects the article DOI: 10.1371/journal.ppat.1005684.].
February 2017: PLoS Pathogens
https://www.readbyqxmd.com/read/28199388/occurrence-of-novel-gii-17-and-gii-21-norovirus-variants-in-the-coastal-environment-of-south-korea-in-2015
#12
Eung Seo Koo, Man Su Kim, Yong Seon Choi, Kwon-Sam Park, Yong Seok Jeong
Human norovirus (HNoV), a positive-sense RNA virus, is the main causative agent of acute viral gastroenteritis. Multiple pandemic variants of the genogroup II genotype 4 (GII.4) of NoV have attracted great attention from researchers worldwide. However, novel variants of GII.17 have been overtaking those pandemic variants in some areas of East Asia. To investigate the environmental occurrence of GII in South Korea, we collected water samples from coastal streams and a neighboring waste water treatment plant in North Jeolla province (in March, July, and December of 2015)...
2017: PloS One
https://www.readbyqxmd.com/read/28198556/comparison-of-genetic-characteristics-in-the-evolution-of-norovirus-gii-4-and-gii-17
#13
Mori Kohji, Motomura Kazushi, Somura Yoshiko, Kimoto Kana, Akiba Tetsuya, Sadamasu Kenji
The genetic characteristics of Norovirus GII.17 were evaluated. Phylogenetic analysis and comparisons of amino acid (Aa) substitutions and nonsynonymous (NS) substitutions/site/year were performed. The complete VP1 sequence of Tokyo/27-3/1976 clustered independently with GII.P17_GII.17 strains. Aa substitutions were mainly accumulated in the P2 domain. NS substitutions/site/year for Tokyo/27-3/1976 compared to Kawasaki323/2014 and Kawasaki308/2015 were 0.57 × 10(-3) and 0.78 × 10(-3) , respectively; for GII...
February 15, 2017: Journal of Medical Virology
https://www.readbyqxmd.com/read/28197973/assessment-and-evaluation-of-an-integrated-hybrid-anaerobic-aerobic-sewage-treatment-system-for-the-removal-of-enteric-viruses
#14
Waled Morsy El-Senousy, Sohair Imam Abou-Elela
The capability of a cost-effective and a small size decentralized pilot wastewater treatment plant (WWTP) to remove enteric viruses such as rotavirus, norovirus genogroup I (GGI), norovirus genogroup II (GGII), Hepatitis E virus (HEV), and adenovirus was studied. This pilot plant is an integrated hybrid anaerobic/aerobic setup which consisted of anaerobic sludge blanket (UASB), biological aerated filter (BAF), and inclined plate settler (IPS). Both the UASB and BAF are packed with a non-woven polyester fabric (NWPF)...
February 14, 2017: Food and Environmental Virology
https://www.readbyqxmd.com/read/28197136/characterization-of-the-genomic-diversity-of-norovirus-in-linked-patients-using-a-metagenomic-deep-sequencing-approach
#15
Neda Nasheri, Nicholas Petronella, Jennifer Ronholm, Sabah Bidawid, Nathalie Corneau
Norovirus (NoV) is the leading cause of gastroenteritis worldwide. A robust cell culture system does not exist for NoV and therefore detailed characterization of outbreak and sporadic strains relies on molecular techniques. In this study, we employed a metagenomic approach that uses non-specific amplification followed by next-generation sequencing to whole genome sequence NoV genomes directly from clinical samples obtained from 8 linked patients. Enough sequencing depth was obtained for each sample to use a de novo assembly of near-complete genome sequences...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28196637/an-outbreak-of-acute-gii-17-norovirus-gastroenteritis-in-a-long-term-care-facility-in-china-the-role-of-nursing-assistants
#16
Chang Cui, Lifeng Pan, Yuanping Wang, Caoyi Xue, Weiping Zhu, Linying Zhu, Chuchu Ye, Xianghua Lu, Haihong Song, Yifei Fu, Qiao Sun
More than 30 residents and nursing assistants in a geriatric nursing hospital developed acute gastroenteritis from December 7th to December 18th, 2014 in Shanghai, China. An immediate epidemiological investigation was conducted to identify the etiological agent of the outbreak, mode of transmission and the risk factors. Cases were investigated according to an epidemiological questionnaire. Samples from cases, highly transmissible environmental surfaces and drinking water were collected for pathogens detection...
February 9, 2017: Journal of Infection and Public Health
https://www.readbyqxmd.com/read/28186546/case-control-assessment-of-the-roles-of-noroviruses-human-bocaviruses-2-3-and-4-and-novel-polyomaviruses-and-astroviruses-in-acute-childhood-diarrhea
#17
Rimma Melamed, Gregory A Storch, Lori R Holtz, Eileen J Klein, Brittany Herrin, Phillip I Tarr, Donna M Denno
No abstract text is available yet for this article.
February 10, 2017: Journal of the Pediatric Infectious Diseases Society
https://www.readbyqxmd.com/read/28185831/use-of-dual-priming-oligonucleotide-system-based-multiplex-rt-pcr-combined-with-high-performance-liquid-chromatography-assay-for-simultaneous-detection-of-five-enteric-viruses-associated-with-acute-enteritis
#18
Wen-Lu Fan, Zi-Wei Wang, Yue Qin, Chao Sun, Zhong-Mei Liu, Yan-Ping Jiang, Xin-Yuan Qiao, Li-Jie Tang, Yi-Jing Li, Yi-Gang Xu
In this study, a specific and sensitive method for simultaneous detection of human astrovirus, human rotavirus, norovirus, sapovirus and enteric adenovirus associated with acute enteritis was developed, based on the specific dual priming oligonucleotide (DPO) system and the sensitive high-performance liquid chromatography (HPLC) analysis. The DPO system-based multiplex reverse transcription-polymerase chain reaction (RT-PCR) combined with HPLC assay was more sensitive than agarose gel electrophoresis analysis and real-time SYBR Green PCR assay, and showed a specificity of 100% and sensitivity of 96%-100%...
February 6, 2017: Journal of Virological Methods
https://www.readbyqxmd.com/read/28181902/steep-rise-in-norovirus-cases-and-emergence-of-a-new-recombinant-strain-gii-p16-gii-2-germany-winter-2016
#19
S Niendorf, S Jacobsen, M Faber, A M Eis-Hübinger, J Hofmann, O Zimmermann, M Höhne, C T Bock
Since early November 2016, the number of laboratory-confirmed norovirus infections reported in Germany has been increasing steeply. Here, we report the detection and genetic characterisation of an emerging norovirus recombinant, GII.P16-GII.2. This strain was frequently identified as the cause of sporadic cases as well as outbreaks in nine federal states of Germany. Our findings suggest that the emergence of GII.P16-GII.2 contributed to rising case numbers of norovirus gastroenteritis in Germany.
January 26, 2017: Euro Surveillance: Bulletin Européen sur les Maladies Transmissibles, European Communicable Disease Bulletin
https://www.readbyqxmd.com/read/28181155/genetic-analysis-of-norovirus-strains-that-caused-gastroenteritis-outbreaks-among-river-rafters-in-the-grand-canyon-arizona
#20
Masaaki Kitajima, Brandon C Iker, Anne Magill-Collins, Marlene Gaither, James D Stoehr, Charles P Gerba
Toilet solid waste samples collected from five outbreaks among rafters in the Grand Canyon were subjected to sequencing analysis of norovirus partial capsid gene. The results revealed that a GI.3 strain was associated with one outbreak, whereas the other outbreaks were caused by GII.5 whose sequences shared >98.9% homology.
February 8, 2017: Food and Environmental Virology
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