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CD8 T helper

Yu Gao, E A Postovalova, O V Makarova, M T Dobrynina, L P Mikhailova
Morphological manifestations of acute colitis and subpopulation composition of colon lymphocytes were studied in male and female C57Bl/6 mice with acute dextran-induced colitis. We evaluated the severity of colitis symptoms, morphological changes in the colon, and prevalence of epithelialized and non-epithelialized ulcers. The subpopulation composition of lymphocytes (CD3-CD19+ B cells, CD3+ CD4+ T helpers, CD3+ CD8+ cytotoxic T cells, and CD4+ CD25+ FOXP3+ regulatory T cells) was assessed by flow cytofluorometry in suspension of colon cells prepared by enzymatic disintegration...
August 18, 2018: Bulletin of Experimental Biology and Medicine
Heba Ahmed Osman, Sanaa S Aly, Hasan S Mahmoud, Eman H Ahmed, Eman M Salah Eldin, Eman A Abdelrahim, Muhammad A El Masry, Rania A Herdan, Mohammed H Hassan
BACKGROUND AND AIMS: Gastroesophageal reflux disease (GERD) is a common prevalent disease. We aimed to assess the dynamic changes in the peripheral T lymphocytes and lymphocytes infiltrating the esophageal mucosa after treatment with proton-pump inhibitor (PPI) in patients with GERD. PATIENTS AND METHODS: A total of 200 patients who presented with upper GIT symptoms were included in this prospective study. All patients were subjected to full history taking, clinical examination, and complete blood count...
August 16, 2018: Journal of Clinical Gastroenterology
Yun Jung Choi, Hi-Jung Park, Hye Jin Park, Kyeong Cheon Jung, Jae-Il Lee
Peripheral CD4/CD8 double-positive (DP) T cells are associated with autoimmune disorders, cancer, and viral infection. However, the relationship between organ transplantation and DP T cells is unclear. Here, we examined the functional characteristics of peripheral DP T cells and analyzed their significance with respect to islet graft rejection in a nonhuman primate model of islet transplantation. DP T cells were functionally equivalent to conventional CD4 and CD8 T cells in terms of helper and cytotoxic activity, respectively...
2018: Journal of Immunology Research
Antonella Frattari, Ennio Polilli, Vanessa Primiterra, Vincenzo Savini, Tamara Ursini, Giancarlo Di Iorio, Giustino Parruti
A better knowledge of factors predicting the development of sepsis in patients hospitalized in intensive care unit (ICU) might help deploy more targeted preventive and therapeutic strategies. In addition to the known clinical and demographic predictors of septic syndromes, in this study, we investigated whether measuring T and B lymphocyte subsets upon admission in the ICU may help individualize the prediction of ensuing sepsis during ICU stay. Between May 2015 and December 2016, we performed a prospective cohort study evaluating peripheral blood lymphocyte T-CD4+ (T-helper cells), T-CD8+ (cytotoxic T-cells), T-CD56 + (natural killer cells), and T-CD19+ (B-lymphocytes), using flow cytometry on blood samples collected 2 days after admission in the ICU...
January 2018: International Journal of Immunopathology and Pharmacology
Michelle Miron, Brahma V Kumar, Wenzhao Meng, Tomer Granot, Dustin J Carpenter, Takashi Senda, Dora Chen, Aaron M Rosenfeld, Bochao Zhang, Harvey Lerner, Amy L Friedman, Uri Hershberg, Yufeng Shen, Adeeb Rahman, Eline T Luning Prak, Donna L Farber
Translating studies on T cell function and modulation from mouse models to humans requires extrapolating in vivo results on mouse T cell responses in lymphoid organs (spleen and lymph nodes [LN]) to human peripheral blood T cells. However, our understanding of T cell responses in human lymphoid sites and their relation to peripheral blood remains sparse. In this study, we used a unique human tissue resource to study human T cells in different anatomical compartments within individual donors and identify a subset of memory CD8+ T cells in LN, which maintain a distinct differentiation and functional profile compared with memory CD8+ T cells in blood, spleen, bone marrow, and lungs...
August 15, 2018: Journal of Immunology: Official Journal of the American Association of Immunologists
Amédée Renand, Sarah Habes, Jean-François Mosnier, Hélène Aublé, Jean-Paul Judor, Nicolas Vince, Philippe Hulin, Steven Nedellec, Sylvie Métairie, Isabelle Archambeaud, Sophie Brouard, Jérôme Gournay, Sophie Conchon
Autoimmune hepatitis (AIH) is a rare disease characterized by an immune attack of the liver. This study consists of a comprehensive analysis of immune alterations related to AIH at diagnosis, and during remission phase under treatment. A total of 37 major lymphocyte populations were analyzed from the peripheral blood of new-onset AIH patients (AIHn; n = 14), AIH patients with controlled disease (n = 11), and healthy subjects (n = 14). Liver biopsy analyses were performed to complete the blood phenotypic analysis...
August 2018: Hepatology Communications
Tiehui Wang, Yehfang Hu, Eakapol Wangkahart, Fuguo Liu, Alex Wang, Eman Zahran, Kevin R Maisey, Min Liu, Qiaoqing Xu, Mónica Imarai, Christopher J Secombes
Mammalian interleukin (IL)-2 is a cytokine centrally involved in the differentiation and survival of CD4+ T helper subsets and CD4+ T regulatory cells and in activation of cytotoxic effector lymphocytes. In bony fish, IL2 orthologs have been identified with an additional divergent IL2-Like gene on the same locus present in several fish species. We report here two divergent IL2 paralogs, IL2A and IL2B , in salmonids that originated from the whole genome duplication event in this fish lineage. The salmonid IL2 paralogs differ not only in sequence but also in exon sizes...
2018: Frontiers in Immunology
P C Gaspar, R C M Rudolf-Oliveira, L M Wildner, A C R de Moraes, M L Reis, R M da Silva, J Gonçalves, M L Bazzo, M C Santos-Silva
SETTING: Information about the sputum cells of pulmonary tuberculosis (PTB) patients is scarce. The analysis of sputum cells using optical microscopy (OM) is a well-established method, but it has some serious limitations. OBJECTIVE: To establish a new flow cytometry (FC) protocol for the leucocyte evaluation of sputum samples from PTB patients. DESIGN: A new FC protocol using 0.1% dithiothreitol and 0.5% paraformaldehyde was developed to fluidise sputum samples and kill Mycobacterium tuberculosis, respectively, to allow the analysis of sputum samples collected from TB patients...
September 1, 2018: International Journal of Tuberculosis and Lung Disease
Kieu-Suong Le, Patricia Amé-Thomas, Karin Tarte, Françoise Gondois-Rey, Samuel Granjeaud, Florence Orlanducci, Etienne D Foucher, Florence Broussais, Reda Bouabdallah, Thierry Fest, Dominique Leroux, Sapna Yadavilli, Patrick A Mayes, Luc Xerri, Daniel Olive
A better characterization of T-cell subsets in the microenvironment of classical Hodgkin lymphoma (cHL) would help to develop immunotherapies. Using multicolor flow cytometry, we identified in 6 of 43 cHL tissue samples a previously unrecognized subset of CD8 T cells coexpressing CXCR5 and inducible T-cell costimulator (ICOS) molecules (CD8CXCR5+ICOS+ ). These cells shared phenotypic features with follicular helper T (TFH ) cells including low CCR7 expression together with high expression of B-cell lymphoma-6, programmed cell death 1, B and T lymphocyte attenuator, CD200, and OX40...
August 14, 2018: Blood Advances
Franz-Tassilo Müller-Graff, Brit Fitzner, Robert Jaster, Brigitte Vollmar, Dietmar Zechner
AIM: To evaluate the influence of hyperglycemia on the progression of autoimmune pancreatitis. METHODS: We induced hyperglycemia by repetitive intraperitoneal (ip) injection of 50 mg/kg streptozotocin in MRL/MpJ mice, which develop autoimmune pancreatitis due to a genetic predisposition. We compared the extent of inflammation (histological score, CD3+ lymphocytes, CD8+ T-cells, CD4+ T-cells, Foxp3+ T-helper cells) in the pancreas of hyperglycemic and normoglycemic mice...
July 28, 2018: World Journal of Gastroenterology: WJG
L Roncati, A Manenti, G Barbolini, A Maiorana
The histology of signet-ring cell carcinoma (SRC) of the stomach has been revisited with the support of current immuno- histochemical techniques in order to explain particular features of this tumor; its great capacity of local diffusion and lymph node metastasis, also through a neo-lymphoangiogenesis. An observational retrospective study on 50 cases of SRC in stage II and III has been performed with the addition of histochemical (Alcian Blue, DDD-Fast Blue B, Mercury Orange) and immunohistochemical (cytocheratin, CD3, CD4, CD8, CD10, CD56, CD68, perforin, granzyme B, podoplanin, collagen type IV) investigations for each case...
2018: Neoplasma
Chao-Yuan Hsu, Li-Tzu Yeh, Shin-Huei Fu, Ming-Wei Chien, Yu-Wen Liu, Shi-Chuen Miaw, Deh-Ming Chang, Huey-Kang Sytwu
SUMOylation is involved in the development of several inflammatory diseases, but the physiological significance of SUMO-modulated c-Maf in autoimmune diabetes is not completely understood. Here, we report that an age-dependent attenuation of c-Maf SUMOylation in CD4+ T cells is positively correlated with the IL-21-mediated diabetogenesis in NOD mice. Using 2 strains of T cell-specific transgenic NOD mice overexpressing wild-type c-Maf (Tg-WTc) or SUMOylation site-mutated c-Maf (Tg-KRc), we demonstrated that Tg-KRc mice developed diabetes more rapidly than Tg-WTc mice in a CD4+ T cell-autonomous manner...
July 30, 2018: Journal of Clinical Investigation
Éric Toussirot, Caroline Laheurte, Béatrice Gaugler, Damien Gabriel, Philippe Saas
The IL-23/T helper 17 (Th17) axis plays an important role in joint inflammation in ankylosing spondylitis (AS). Conventional CD4+ Th17 cells are a major source of IL-17A. IL-22 is another cytokine implicated in AS pathophysiology and is produced by Th17 and Th22 cells. In this study, we aimed to analyze conventional and non-conventional T cell subsets producing IL-17A and IL-22 in patients with AS. We thus evaluated the intracellular staining for IL-17A, IL-22, and IFN-γ in peripheral blood mononuclear cells of 36 patients with AS and 55 age- and sex-matched healthy controls (HC)...
2018: Frontiers in Immunology
Wenqiang Jiang, Wenhong Zhong, Yiyu Deng, Chunbo Chen, Qiaosheng Wang, Maohua Zhou, Xusheng Li, Cheng Sun, Hongke Zeng
BACKGROUND: A major challenge in sepsis intervention is unclear risk stratification. We postulated that a panel of biomarkers of lymphocyte apoptosis and immune function, termed the "lymphocyte apoptosis model," would be an effective tool for predicting 28-day survival for sepsis patients. METHODS: A total of 52 consecutive sepsis patients were enrolled. Peripheral blood samples were collected on day 1 of admission for quantification of biomarkers of lymphocyte apoptosis and immune function, including lymphocyte count, lymphocyte apoptotic percentage, expression on monocyte HLA-DR, CD4+ /CD8+ T cell ratio, T helper type 1 to type 2 ratio (Th1/Th2), cytochrome c levels, and various proinflammatory cytokine levels...
July 18, 2018: BMC Anesthesiology
Hiroaki Suzuki, Kenta Jounai, Konomi Ohshio, Toshio Fujii, Daisuke Fujiwara
Lactic acid bacteria (LAB) have been reported to have beneficial effects on protective immunity against viruses and pathogenic bacteria by activating innate immune cells such as dendritic cells (DC) or macrophages. However, little is known about whether LAB contributes to antigen-specific immune responses. Because plasmacytoid DC (pDC) links innate and acquired immunity, here we investigated whether the pDC-stimulative LAB, Lactococcus lactis strain Plasma (LC-Plasma), influences antigen-specific immune responses...
July 11, 2018: Biochemical and Biophysical Research Communications
Hong-Min Wang, Xiao-Hong Zhang, Ming-Ming Feng, Yan-Jun Qiao, Li-Qun Ye, Jing Chen, Fei-Fei Fan, Lin-Lin Guo
BACKGROUND/AIMS: Interleukin (IL)-35 has immunosuppressive functions in autoimmune diseases, infectious diseases, and certain cancers. However, few studies have focused on its immunoregulatory activity in non-small cell lung cancer (NSCLC). Thus, we investigated the role of IL-35 in the pathogenesis of this disease. METHODS: A total of 66 NSCLC patients and 21 healthy individuals were enrolled. IL-35 expression in peripheral blood and bronchoalveolar lavage fluid (BALF) was measured...
2018: Cellular Physiology and Biochemistry
Nianqiu Liu, Bin Liu, Li Zhang, Hu Li, Zhiwei Chen, Aoran Luo, Min Chen, Mingli Peng, Wenwei Yin, Hong Ren, Peng Hu
BACKGROUND AND AIMS: Much evidence indicates that the soluble antigens secreted by hepatitis B virus (HBV) inhibit the function of the immune system. The aim of this study is to investigate, after treatment with nucleoside (acid) analogs (NAs) and the inhibition of viral replication, whether the immune systems of patients with a peripheral blood HBV-DNA level <1000 IU/mL, hepatitis B e antigen (HBeAg) disappearance, and a decrease in hepatitis B surface antigen (HBsAg) levels could be reconstructed...
July 4, 2018: International Immunopharmacology
Katarzyna Dudek, Dariusz Bednarek
Introduction: Mycoplasma bovis is a well-known cause of various disorders in cattle, such as pneumonia, arthritis, mastitis kerato-conjunctivitis, pharyngitis, laryngitis, otitis media, meningitis, and reproductive disorders. There are no commercial vaccines against M. bovis in Europe, therefore, experimental ones are still under investigation. The aim of this study was to evaluate the effect of experimental M. bovis vaccine, containing the Polish field M. bovis strain as well as saponin and lysozyme dimer adjuvants, on the T- and B-cell response in calves...
December 2017: Journal of Veterinary Research
Heather Evans-Marin, Rebecca Rogier, Sergei B Koralov, Julia Manasson, Debbie Roeleveld, Peter M van der Kraan, Jose U Scher, Marije I Koenders, Shahla Abdollahi-Roodsaz
OBJECTIVE: The intestinal microbiota has been associated with the development of inflammatory arthritis. The aim of this study was to dissect intestinal mucosal immune responses in the preclinical phase of arthritis and determine the requirement of Th17 cells, beyond the cytokine interleukin-17 (IL-17), for arthritis development. We further examined whether the involvement of Th17 cells in arthritis depends on the host microbiota. METHODS: We analyzed mucosal T cell production of IL-17, IFNγ, TNFα, IL-22 and GM-CSF by flow cytometry and Luminex in mice immunized for the induction of collagen-induced arthritis (CIA) before arthritis onset...
July 5, 2018: Arthritis & Rheumatology
Z J Zhang, L T Ding, J Zou, G Z Lyu
Objective: To explore the changes of helper T lymphocytes 17 (Th17) and regulatory T lymphocytes (Tregs) in peripheral blood of patients with extensive burn at early stage in August 2nd aluminum dust explosion accident in Kunshan factory (hereinafter referred to as Kunshan explosion accident) and the significance. Methods: Twenty patients with extensive burn in Kunshan explosion accident admitted to our department of burns and plastic surgery were enrolled in burn group, and 10 healthy adult volunteers with no history of smoking were enrolled in healthy control group...
June 20, 2018: Zhonghua Shao Shang za Zhi, Zhonghua Shaoshang Zazhi, Chinese Journal of Burns
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