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https://www.readbyqxmd.com/read/30525551/an-evaluation-of-hair-oral-fluid-earwax-and-nasal-mucus-as-noninvasive-specimens-to-determine-livestock-exposure-to-teratogenic-lupine-spp
#1
Stephen T Lee, Clinton A Stonecipher, Fabricio C Dos Santos, James A Pfister, Kevin D Welch, Daniel Cook, Benedict T Green, Dale R Gardner, Kip E Panter
The livestock industry in the western United States loses an estimated $500 million annually from livestock production losses due to poisonous plants. Poisoning of livestock by plants often goes undiagnosed because there is a lack of appropriate or available specimens for analysis. Lupinus species represent an important toxic plant in western North America that can be toxic and/or teratogenic to livestock species due to the quinolizidine alkaloids. The objective of this study was to evaluate the potential of using earwax, hair, oral fluid, and nasal mucus as noninvasive specimens to determine livestock exposure to teratogenic Lupinus species...
December 7, 2018: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/30501016/living-with-alopecia-areata-an-online-qualitative-survey-study
#2
L Davey, V Clarke, E Jenkinson
BACKGROUND: Living with alopecia areata, totalis and universalis (collectively referred to here as AA) involves unpredictable, sometimes rapid hair loss. There is currently no effective treatment and patients describe feelings of shock, loss, trauma and disrupted identity. Cultural meanings attached to hair and hair loss, including associations between hair and femininity, and hair loss and cancer may exacerbate distress. Consequently, wigs and make-up are frequently used as camouflage, but this can produce feelings of inauthenticity, shame and anxiety...
November 30, 2018: British Journal of Dermatology
https://www.readbyqxmd.com/read/30387241/lipids-source-of-static-electricity-of-regenerative-natural-substances-and-nondestructive-energy-harvesting
#3
Dong Wook Kim, Sang-Woo Kim, Unyong Jeong
It is familiar to everyone that human skin and hair easily lose electrons and cause static electricity as they undergo friction with other materials. Such natural regenerative substances take a high ranking in the triboelectric series. Even though the static electricity of regenerative natural substances has been a long-term curiosity in human history, it is not yet clear which of their components causes the positive static charges. This study reveals that lipid layers on the surface of regenerative substances (skin, hair, leaves, cells) and even synthetic lipids are responsible for this positive static electricity and shows that it is possible to manufacture lipid-based triboelectric nanogenerators (TENGs)...
November 2, 2018: Advanced Materials
https://www.readbyqxmd.com/read/30253942/restoration-of-hair-inductive-activity-of-cultured-human-follicular-keratinocytes-by-co-culturing-with-dermal-papilla-cells
#4
Soon Sun Bak, Mi Hee Kwack, Hyun Su Shin, Jung Chul Kim, Moon Kyu Kim, Young Kwan Sung
Hair follicle outer root sheath (ORS) cells can be expanded in vitro, but often lose receptivity to hair-inducing dermal signals. Recent studies have shown hair-inductive activity (trichogenicity) can be restored in rat ORS cells expanded with a fibroblast feeder by co-culturing with rat vibrissae dermal papilla (DP) cells. In this study, we investigated whether the trichogenicity of human ORS cells can be restored by co-culturing with human DP cells. ORS cells from human scalp hair follicles were cultured independently or with DP cells for 5 days and implanted into nude mice alongside freshly isolated neonatal mouse dermal cells...
October 28, 2018: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/30232392/light-cured-polymer-electrodes-for-non-invasive-eeg-recordings
#5
Nora Vanessa de Camp, Gerhard Kalinka, Jürgen Bergeler
We invented the first non-metallic, self-adhesive and dry biosignalling electrode. The PEDOT polymer electrode changes its aggregate state and conductivity by a light curing procedure. The electrode can be applied as a gel underneath hair without shaving. With the aid of blue light, the electrode can be hardened within a few seconds at the desired location on the scalp. The cured polymer electrode is highly conductive and can be applied on a very small location. Unlike other EEG electrodes, our electrode does not lose conductivity upon drying...
September 19, 2018: Scientific Reports
https://www.readbyqxmd.com/read/30123244/inner-ear-hair-cell-protection-in-mammals-against-the-noise-induced-cochlear-damage
#6
REVIEW
Muhammad Waqas, Song Gao, Iram-Us-Salam, Muhammad Kazim Ali, Yongming Ma, Wenyan Li
Inner ear hair cells are mechanosensory receptors that perceive mechanical sound and help to decode the sound in order to understand spoken language. Exposure to intense noise may result in the damage to the inner ear hair cells, causing noise-induced hearing loss (NIHL). Particularly, the outer hair cells are the first and the most affected cells in NIHL. After acoustic trauma, hair cells lose their structural integrity and initiate a self-deterioration process due to the oxidative stress. The activation of different cellular death pathways leads to complete hair cell death...
2018: Neural Plasticity
https://www.readbyqxmd.com/read/29942920/suspected-testosterone-producing-tumor-in-a-patient-taking-biotin-supplements
#7
Heather M Stieglitz, Nichole Korpi-Steiner, Brooke Katzman, Jennifer E Mersereau, Maya Styner
A perimenopausal woman presented with palpitations, hirsutism, and inability to lose weight. Laboratory tests revealed an unusual endocrine hormonal profile including pituitary hormones (TSH, ACTH, and prolactin) below reference intervals and gonadal (testosterone) and adrenal (cortisol) hormones above reference intervals. Ultimately, after a comprehensive workup including a scheduled surgical procedure, abnormal laboratories were determined due to biotin interference. Biotin (vitamin B7) is a water-soluble vitamin and essential cofactor for the metabolism of fatty acids, glucose, and amino acids...
June 1, 2018: Journal of the Endocrine Society
https://www.readbyqxmd.com/read/29687797/impact-of-substrate-stiffness-on-dermal-papilla-aggregates-in-microgels
#8
Justin J Y Tan, Jie Kai Tee, Keat Onn Chou, Shi Ya Au Yong, Jing Pan, Han Kiat Ho, Paul C L Ho, Lifeng Kang
Interaction between cells and the extracellular environment plays a vital role in cellular development. The mechanical property of a 3-dimensional (3D) culture can be modified to mimic in vivo conditions. Dermal papilla (DP) cells are shown to gradually lose their inductivity in hair cycle development in a 2-dimensional culture. They are shown to partially restore their inductivity when transferred into a 3D microenvironment. In this study, a microarray fabricated from three different concentrations of poly-ethylene-glycol-diacrylate 3500, namely 5%, 10% and 15% w/v, yielded increasing substrate stiffness...
May 29, 2018: Biomaterials Science
https://www.readbyqxmd.com/read/29602151/usefulness-of-hair-analysis-and-psychological-tests-for-identification-of-alcohol-and-drugs-of-abuse-consumption-in-driving-license-regranting
#9
Elena Lendoiro, Ana de Castro, Cristian Jiménez-Morigosa, Xosé A Gomez-Fraguela, Manuel López-Rivadulla, Angelines Cruz
The implementation of the points-based driving license helps to change the drivers' behavior and is related to a reduction of traffic accidents and fatalities. In Spain, when a driver loses all points, the driving license is revoked, so the driver must enroll on a Driver Awareness and Re-education (DARE) course. However, at the moment offenders are not submitted to any test to confirm absence of alcohol or drugs of abuse consumption, even when 9% of Spanish drivers lose their driving license for driving under the influence (DUI)...
May 2018: Forensic Science International
https://www.readbyqxmd.com/read/29589644/congenital-atrichia-with-papular-lesions-a-rare-cause-of-irreversible-childhood-alopecia
#10
Tasleem Arif, Mohammad Adil, Syed Suhail Amin
Congenital atrichia with papular lesions (APL) is a disease characterized by the complete absence of hair from the whole body occurring within a few months of birth and the presence of papules distributed over the body. A 9-year-old boy presented with sparse hair over his body and with a history of losing his hair soon after birth. Multiple skin-colored papules were observed over the scalp. A biopsy from a papule showed keratinous cysts in the dermis and the absence of hair follicles. A diagnosis of congenital APL was made after vitamin D-dependent rickets was excluded...
March 2018: Acta Dermatovenerologica Alpina, Panonica, et Adriatica
https://www.readbyqxmd.com/read/29584689/extracellular-matrix-as-a-regulator-of-epidermal-stem-cell-fate
#11
REVIEW
Elina Chermnykh, Ekaterina Kalabusheva, Ekaterina Vorotelyak
Epidermal stem cells reside within the specific anatomic location, called niche, which is a microenvironment that interacts with stem cells to regulate their fate. Regulation of many important processes, including maintenance of stem cell quiescence, self-renewal, and homeostasis, as well as the regulation of division and differentiation, are common functions of the stem cell niche. As it was shown in multiple studies, extracellular matrix (ECM) contributes a lot to stem cell niches in various tissues, including that of skin...
March 27, 2018: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/29033351/mouse-cutaneous-melanoma-induced-by-mutant-braf-arises-from-expansion-and-dedifferentiation-of-mature-pigmented-melanocytes
#12
Corinna Köhler, David Nittner, Florian Rambow, Enrico Radaelli, Fabio Stanchi, Niels Vandamme, Arianna Baggiolini, Lukas Sommer, Geert Berx, Joost J van den Oord, Holger Gerhardt, Cedric Blanpain, Jean-Christophe Marine
To identify the cells at the origin of melanoma, we combined single-cell lineage-tracing and transcriptomics approaches with time-lapse imaging. A mouse model that recapitulates key histopathological features of human melanomagenesis was created by inducing a BRafV600E-driven melanomagenic program in tail interfollicular melanocytes. Most targeted mature, melanin-producing melanocytes expanded clonally within the epidermis before losing their differentiated features through transcriptional reprogramming and eventually invading the dermis...
November 2, 2017: Cell Stem Cell
https://www.readbyqxmd.com/read/29030818/identifying-quiescent-stem-cells-in-hair-follicles
#13
Christine N Rodriguez, Hoang Nguyen
Hair follicle stem cells (HFSCs) are noted for their relative quiescence and therefore can be distinguished from other cells by their differential history of cell division. Replicating cells can be labeled by pulsing the animals repeatedly with 5-bromo-2'-deoxyuridine (BrdU) or tritiated thymidine ([3 H]TdR), thymidine analogs that get incorporated into DNA during DNA synthesis. Because dividing cells dilute the label after each cell division, frequently dividing cells will lose the label over time while slow cycling cells will retain the label and thus are termed label retaining cells (LRCs)...
2018: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28893539/functional-analysis-of-a-sox10-gene-mutation-associated-with-waardenburg-syndrome-ii
#14
Xue-Ping Wang, Zi-Qi Hao, Ya-Lan Liu, Ling-Yun Mei, Chu-Feng He, Zhi-Jie Niu, Jie Sun, Yu-Lin Zhao, Yong Feng
Waardenburg syndrome (WS) is an autosomal dominant inherited non-syndromic type of hereditary hearing loss characterized by varying combinations of sensorineural hearing loss and abnormal pigmentation of the hair, skin, and inner ear. WS is classified into four subtypes (WS1-WS4) based on additional symptoms. WS2 is characterized by the absence of additional symptoms. Recently, we identified a SOX10 missense mutation c.422T > C (p.L141P) associated with WS2. We performed functional assays and found the mutant loses DNA-binding capacity, shows aberrant cytoplasmic and nuclear localization, and fails to interact with PAX3...
November 4, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/28875267/kingdom-chromista-and-its-eight-phyla-a-new-synthesis-emphasising-periplastid-protein-targeting-cytoskeletal-and-periplastid-evolution-and-ancient-divergences
#15
Thomas Cavalier-Smith
In 1981 I established kingdom Chromista, distinguished from Plantae because of its more complex chloroplast-associated membrane topology and rigid tubular multipartite ciliary hairs. Plantae originated by converting a cyanobacterium to chloroplasts with Toc/Tic translocons; most evolved cell walls early, thereby losing phagotrophy. Chromists originated by enslaving a phagocytosed red alga, surrounding plastids by two extra membranes, placing them within the endomembrane system, necessitating novel protein import machineries...
January 2018: Protoplasma
https://www.readbyqxmd.com/read/28870992/cell-migration-intercalation-and-growth-regulate-mammalian-cochlear-extension
#16
Elizabeth Carroll Driver, Amy Northrop, Matthew W Kelley
Developmental remodeling of the sensory epithelium of the cochlea is required for the formation of an elongated, tonotopically organized auditory organ, but the cellular processes that mediate these events are largely unknown. We used both morphological assessments of cellular rearrangements and time-lapse imaging to visualize cochlear remodeling in mouse. Analysis of cell redistribution showed that the cochlea extends through a combination of radial intercalation and cell growth. Live imaging demonstrated that concomitant cellular intercalation results in a brief period of epithelial convergence, although subsequent changes in cell size lead to medial-lateral spreading...
October 15, 2017: Development
https://www.readbyqxmd.com/read/28683402/different-roles-of-glutathione-in-copper-and-zinc-chelation-in-brassica-napus-roots
#17
Ilya E Zlobin, Alexander V Kartashov, George V Shpakovski
We investigated the specific features of copper and zinc excess action on the roots of canola (Brassica napus L.) plants. Copper rapidly accumulated in canola root cells and reached saturation during several hours of treatment, whereas the root zinc content increased relatively slowly. Excessive copper and zinc entry inside the cell resulted in significant cell damage, as evidenced by alterations in plasmalemma permeability and decreases in cellular enzymatic activity. Zinc excess specifically damaged root hair cells, which correlated with a pronounced elevation of their labile zinc level...
September 2017: Plant Physiology and Biochemistry: PPB
https://www.readbyqxmd.com/read/28551734/alopecia-areata-an-update-on-treatment-options-for-children
#18
REVIEW
Lauren Peloquin, Leslie Castelo-Soccio
Pediatric alopecia areata is a spectrum of autoimmune non-scarring alopecia in which some patients lose small patches of hair from their scalp but others lose more or all of the hair from the scalp and body, including eyebrows and eyelashes. Few studies have looked at therapies for this disorder in children, so much of the data are derived from adult literature and describe off-label use of medication. Generally, topical therapies consisting of topical steroids and topical irritating compounds/contact sensitizers are used...
October 2017: Paediatric Drugs
https://www.readbyqxmd.com/read/28442778/as-a-carrier-transporter-for-hair-follicle-reconstitution-platelet-rich-plasma-promotes-proliferation-and-induction-of-mouse-dermal-papilla-cells
#19
Shun-E Xiao, Yong Miao, Jin Wang, Wei Jiang, Zhe-Xiang Fan, Xiao-Min Liu, Zhi-Qi Hu
Morphogenesis of hair follicles during development and in hair reconstitution assays involves complex interactions between epithelial cells and dermal papilla cells (DPCs). DPCs may be a source of cells for hair regeneration in alopecia patients. Reconstitution of engineered hair follicles requires in vitro culture of trichogenic cells, a three-dimensional scaffolds, and biomolecular signals. However, DPCs tend to lose their biological activity when cultured as trichogenic cells, and scaffolds currently used for hair follicle regeneration lack biological efficiency and biocompatibility...
April 25, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28330720/attenuation-of-uvr-induced-vitamin-d-3-synthesis-in-a-mouse-model-deleted-for-keratinocyte-lathosterol-5-desaturase
#20
Anastasia M Makarova, Saloni Pasta, Gordon Watson, Cedric Shackleton, Ervin H Epstein
The lower risk of some internal cancers at lower latitudes has been linked to greater sun exposure and consequent higher levels of ultraviolet radiation (UVR)-produced vitamin D3 (D3 ). To separate the experimental effects of sunlight and of all forms of D3 , a mouse in which UVR does not produce D3 would be useful. To this end we have generated mice carrying a modified allele of sterol C5-desaturase (Sc5d), the gene encoding the enzyme that converts lathosterol to 7-dehydrocholesterol (7-DHC), such that Sc5d expression can be inactivated using the Cre/lox site-specific recombination system...
July 2017: Journal of Steroid Biochemistry and Molecular Biology
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