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Circular RNA in HCC

Hongyuan Liu, Li Xue, Changyue Song, Fang Liu, Tingjun Jiang, Xiuhua Yang
Emerging evidence indicated that abnormally expressed circular RNAs (circRNAs) are critically involved in tumorigenesis and development of several cancers. However, the study relevant to the relationship between circRNAs and hepatocellular carcinoma (HCC) is rare. In this study, the expression of circ_001569 in HCC tissue specimens and cells were determined by qRT-PCR. The clinical relevance of circ_001569 was also analyzed. In addition, loss-of-function and gain-of-function assays were conducted to explore the biological functions of circ_001569...
August 4, 2018: Biochemical and Biophysical Research Communications
Man Wang, Fei Yu, Peifeng Li
Hepatocellular carcinoma (HCC) is one of the leading causes of cancer-related deaths worldwide. HCC patients are commonly diagnosed at an advanced stage, for which highly effective therapies are limited. Moreover, the five-year survival rate of HCC patients remains poor due to high frequency of tumor metastasis and recurrence. These challenges give rise to the emergent need to discover promising biomarkers for HCC diagnosis and identify novel targets for HCC therapy. Circular RNAs (circRNAs), a class of long-overlook non-coding RNA, have been revealed as multi-functional RNAs in recent years...
August 2, 2018: Cancers
Ning Bai, Eming Peng, Xingsheng Qiu, Ning Lyu, Zhejia Zhang, Yiming Tao, Xinying Li, Zhiming Wang
BACKGROUD: Accumulating evidences indicate that circular RNAs (circRNAs), a class of non-coding RNAs, play important roles in tumorigenesis. However, the function of circRNAs in hepatocellular cancer (HCC) is largely unknown. METHODS: We performed circRNA microarrays to identify circRNAs that are aberrantly expressed in HCC tissues. Expression levels of a significantly upregulated circRNA, circFBLIM1, was detected by quantitative real-time PCR (qRT-PCR) in HCC cell lines and tissues...
July 27, 2018: Journal of Experimental & Clinical Cancer Research: CR
Zheng Guan, Jing Tan, Wei Gao, Xin Li, Yuandong Yang, Xiaogang Li, Yingchao Li, Qiang Wang
Recent studies have revealed that circular RNAs (circRNAs) play important roles in the tumorigenesis of human cancer, including hepatocellular carcinoma (HCC). In present study, we screen the circular RNA expression profiles in HCC tissue and investigate the molecular roles on HCC tumorigenesis. Human circRNA microarray analysis showed there were total 1,245 differently expressed circular RNAs, including 756 up-regulated circRNAs and 489 down-regulated circRNAs, in three pairs of HCC tissue and adjacent normal tissue...
June 19, 2018: Journal of Cellular Physiology
Baoyong Wang, Hongwei Chen, Chunfen Zhang, Tian Yang, Qiaofei Zhao, Yifan Yan, Yu Zhang, Feng Xu
Primary hepatocellular carcinoma (HCC) is one of the most common malignant tumors. At present, the molecular mechanism of HCC remains unclear. A recent circular RNA (circRNA) profiling study showed that circRBM23 expression was upregulated in HCC tissues. Therefore, in this study, the impact of circRBM23 during the progression of HCC was evaluated. The expression levels of circRBM23 and miR-138 in HCC tissues and HCC cell lines were determined by RT-PCR and the results indicated that circRBM23 expression was increased in the HCC tissues and HCC cell lines, whereas miR-138 expression was decreased...
June 2018: Cancer Biotherapy & Radiopharmaceuticals
Shanshan Wang, Shichang Cui, Wenming Zhao, Zhiling Qian, Hui Liu, Yuhan Chen, Fudong Lv, Hui-Guo Ding
BACKGROUND: Circular RNAs (circRNAs) play an important role in pathogenesis and development of hepatocellular carcinoma (HCC). However, circRNA expression profiles in hepatitis B Virus (HBV)-related HCC remain to be studied. METHODS: Total 13 HBV-related HCC patients were enrolled for study. Three HCC and 3 paired adjacent non-tumorous (NT) tissues from 3 patients were performed for microarray. Ten pairs of HCC tissues were used to verify the identified up-regulated and down-regulated circRNAs obtained from the microarray data by quantitative real-time reverse transcription PCR (qRT-PCR)...
May 29, 2019: Cancer Biomarkers: Section A of Disease Markers
Dawei Chen, Chenyue Zhang, Jiamao Lin, Xinyu Song, Haiyong Wang
Aim: The aim of this study was to analyze the diagnostic and prognostic values of the circular RNA (circRNA) hsa_circ_0128298 in hepatocellular carcinoma (HCC). Patients and methods: The global circRNA expression was measured using circRNA microarray using three pairs of cancer and noncancerous tissues from HCC patients. The microarray analysis revealed that two circRNAs were differentially expressed in the three pairs of cancerous and noncancerous tissues. The higher levels of two representative circRNAs, such as hsa_circ_0128298 and hsa_circ_0091582, were further confirmed by real-time polymerase chain reaction...
2018: Cancer Management and Research
Jing Meng, Shuang Chen, Jingxia Han, Baoxin Qian, Xiaorui Wang, Weilong Zhong, Yuan Qin, Heng Zhang, Wanfeng Gao, Yueyang Lei, Wei Yang, Lan Yang, Chao Zhang, Huijuan Liu, Yanrong Liu, Hong-Gang Zhou, Tao Sun, Cheng Yang
Twist is a critical epithelial-mesenchymal transition (EMT)-inducing transcription factor that increases expression of Vimentin. How Twist1 regulates this expression remains unclear. Here we report that Twist1 regulates Cullin2 (Cul2) circular RNA to increase expression of Vimentin in EMT. Twist1 bound the Cul2 promoter to activate its transcription and selectively promote expression of Cul2 circular RNA (circ-10720), but not mRNA. circ-10720 positively correlated with Twist1, tumor malignance, and poor prognosis in hepatocellular carcinoma (HCC)...
May 29, 2018: Cancer Research
Ting Yao, Qingqing Chen, Zhouwei Shao, Zhihua Song, Liyun Fu, Bingxiu Xiao
BACKGROUND: Circular RNAs (circRNAs) play important roles in disease occurrence. However, the roles of circRNAs in the diagnosis of hepatocellular carcinoma (HCC) are largely unknown. The aim of this study is to investigate the clinical diagnostic values of hsa_circ_0068669 (Alias: hsa_circ_103561), one of the representative HCC-associated circRNAs. METHODS: Hsa_circ_0068669 expression levels in HCC tissues, HCC cell lines, and chronic hepatitis tissues were detected by real-time quantitative reverse transcription-polymerase chain reaction...
May 21, 2018: Journal of Clinical Laboratory Analysis
Xianwei Zhang, Hu Zhou, Wei Jing, Ping Luo, Shili Qiu, Xuefang Liu, Man Zhu, Chunzi Liang, Mingxia Yu, Jiancheng Tu
Circular RNAs (circRNA), a class of noncoding RNAs, have been found to be involved in various diseases. Here, the expression levels of the circRNA hsa_circ_0001445 in 73 pairs of hepatocellular carcinoma (HCC) and adjacent nontumor tissues were investigated by quantitative real-time polymerase chain reaction (qRT-PCR). Our data demonstrate that the hsa_circ_0001445 levels were significantly decreased in HCC tissues ( P < 0.001) and markedly associated with the number of tumor foci ( P = 0.014). Furthermore, in vitro approaches showed that overexpression of hsa_circ_0001445 promoted apoptosis and inhibited proliferation, migration, and invasion of HCC-derived cells, suggesting that hsa_circ_0001445 might be involved in the development of HCC...
2018: Disease Markers
Li-Peng Qiu, Yi-Hang Wu, Xiao-Feng Yu, Qi Tang, Liang Chen, Ke-Ping Chen
Hepatocellular carcinoma (HCC) ranks the third leading cause of cancer death in the world and has a notably low survival rate. Circular RNAs (circRNAs) are newly classed non-coding RNA (ncRNA) members that are capable of regulating gene expression at transcription or post-transcription levels. Recent studies demonstrate that some circRNAs are differentially expressed in HCC, and the deregulation of these circRNAs is associated with the clinical pathological and prognostic significance. They also play essential roles in HCC progression, and contribute to cell proliferation, migration, invasion and metastasis by targeting different microRNAs (miRNAs) and protein-coding genes...
2018: Journal of Cancer
Xiaoming Lin, Yuhan Chen
BACKGROUND Hepatocellular carcinoma (HCC) is the most common malignancy of the liver and recent studies have revealed that circular RNA (circRNA) plays an important role in the pathogenesis of HCC. Some circRNAs may act as a microRNA (miRNA) sponge to affect miRNA activities in the regulation of messenger RNA (mRNA) expression. However, the circRNA-miRNA-mRNA network in HCC remains largely unknown. MATERIAL AND METHODS The circRNA expression profiles (GSE94508 and GSE97332), miRNA and mRNA expression profile (GSE22058) were downloaded from Gene Expression Omnibus microarray data and then a circRNA-miRNA-mRNA regulatory network in HCC was constructed...
April 30, 2018: Medical Science Monitor Basic Research
Weidong Jiang, Dacheng Wen, Lulu Gong, Yu Wang, Zefeng Liu, Fangying Yin
The importance of circular RNAs (circRNAs) in human cancers has gradually been acknowledged. In hepatocellular carcinoma (HCC), several circRNAs have been reported to regulate tumor growth and metastasis. However, the role of hsa_circ_0000673 in HCC remains largely unknown. In this study, we found that hsa_circ_0000673 was significantly upregulated in HCC tissues compared to adjacent non-tumor tissues. Moreover, we found that hsa_circ_0000673 knockdown markedly inhibited the proliferation and invasion of HCC cells in vitro...
June 2, 2018: Biochemical and Biophysical Research Communications
Lihua Zhong, Yuanyuan Wang, Yu Cheng, Wen Wang, Baoling Lu, Liying Zhu, Yingji Ma
In the recent years, increasing evidences identify circular RNAs (circRNAs) as a class of important regulators in various human cancers. Nevertheless, the functions and mechanisms of most circRNAs in cancer cells remain largely unknown. In this study, we found out a significantly downregulated circRNA circC3P1 in hepatocellular carcinoma (HCC) tissues compared to adjacent normal tissues. And our results indicated that circC3P1 expression level was negatively correlated with TNM stage, tumor size and vascular invasion in HCC...
May 23, 2018: Biochemical and Biophysical Research Communications
Miao Wang, Yuxi Yang, Jian Xu, Wen Bai, Xueli Ren, Huijian Wu
BACKGROUND: The expression of circular RNA (circRNA) may affect tumor progression. However, there have been no systemic meta-analysis for cancer diagnosis by using circRNAs in clinical till now. Herein, we aimed to collect and examined all the evidence on the potential role of circRNA as novel biomarker in human cancers. METHODS: A comprehensive search strategy was used to search relevant literatures in the databases of PubMed, Embase, and the Web of Science from 2015 to August 2017...
March 20, 2018: BMC Cancer
Natalia Freitas, Tetyana Lukash, Sumedha Gunewardena, Benjamin Chappell, Betty L Slagle, Severin O Gudima
Five matching sets of nonmalignant liver tissues and hepatocellular carcinoma (HCC) samples from individuals chronically infected with hepatitis B virus (HBV) were examined. The HBV genomic sequences were determined by using overlapping PCR amplicons covering the entire viral genome. Four pairs of tissues were infected with HBV genotype C, while one pair was infected with HBV genotype B. HBV replication markers were found in all tissues. In the majority of HCC samples, the levels of pregenomic/precore RNA (pgRNA) and covalently closed circular DNA (cccDNA) were lower than those in liver tissue counterparts...
May 15, 2018: Journal of Virology
Genwen Chen, Yanting Shi, Mengmeng Liu, Jianyong Sun
Noncoding RNAs plays an important role in hepatocellular carcinoma (HCC). Here, we show that miR-124 was downregulated in HCC tissues and that the ectopic expression of miR-124 inhibited the proliferation and migration of HCC cells. We proposed that aquaporin 3 (AQP3) is a direct target of miR-124. AQP3 was upregulated in HCC tissues and inversely correlated with miR-124 expression. The overexpression of miR-124 decreased AQP3 expression. Indeed, AQP3 overexpression promoted cell proliferation and migration, whereas miR-124 knockdown suppressed cell proliferation and migration...
February 7, 2018: Cell Death & Disease
Shichang Cui, Zhiling Qian, Yuhan Chen, Lei Li, Peng Li, Huiguo Ding
The present study describes circular RNA (circRNA) profiles in three pairs of hepatocellular carcinoma (HCC) tissues and the corresponding adjacent non-tumorous tissues (NTs) by microarray. circRNA is a type of endogenous RNA that serve a crucial role in disease development and aberrantly express in a number of types of cancer. In the present study, 3 paired HCC tissues and paired adjacent NTs were collected from HCC surgical specimens from 3 hepatitis B virus-infected patients with HCC. With abundant and varied probes accounting for 5,396 circRNAs, a large number of circRNAs are able to be quantitatively determined...
January 2018: Oncology Letters
Jian Yu, Qing-Guo Xu, Zhen-Guang Wang, Yuan Yang, Ling Zhang, Jin-Zhao Ma, Shu-Han Sun, Fu Yang, Wei-Ping Zhou
BACKGROUND & AIMS: In recent years, circular RNAs (circRNAs) have been shown to have critical regulatory roles in cancer biology. However, the contributions of circRNAs to hepatocellular carcinoma (HCC) remain largely unknown. METHODS: cSMARCA5 (a circRNA derived from exons 15 and 16 of the SMARCA5 gene, hsa_circ_0001445) was identified by RNA-sequencing and validated by quantitative reverse transcription PCR. The role of cSMARCA5 in HCC progression was assessed both in vitro and in vivo...
June 2018: Journal of Hepatology
Ketao Zhang, Siyao Che, Zheng Su, Shangyou Zheng, Huayao Zhang, Shanglin Yang, Wenda Li, Jianping Liu
Cluster of differentiation (CD)90 (Thy‑1) was proposed as a marker for the liver cancer stem cells that are responsible for tumorigenic activity, however its involvement in the progression of hepatocellular carcinoma (HCC) remains unknown. The aim of the present study was to determine the effect of CD90 on the biological functions of HCC and to investigate the associated circular RNA (circRNA) involved in this process. The analysis of the in vitro data demonstrated that CD90+ cells isolated from SK‑Hep‑1 cells exhibited increased viability, migration and invasive abilities compared with CD90‑ cells...
February 2018: International Journal of Molecular Medicine
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