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https://www.readbyqxmd.com/read/30102770/reconstruction-of-tca-cycle-in-corynebacterium-glutamicum-with-a-genome-scale-metabolic-network-model-for-trans-4-hydroxyproline-production
#1
Yu Zhang, Yun Zhang, Xiuling Shang, Bo Wang, Qitiao Hu, Shuwen Liu, Tingyi Wen
trans-4-Hydroxy-l-proline (Hyp) is an abundant component of mammalian collagen, and functions as a chiral synthon for the syntheses of anti-inflammatory drugs in the pharmaceutical industry. Proline 4-hydroxylase (P4H) can catalyze the conversion of l-proline to Hyp, however, it is still challenging for the fermentative production of Hyp from glucose using P4H due to the low yield and productivity. Here, we report the metabolic engineering of Corynebacterium glutamicum for the fermentative production of Hyp by reconstructing tricarboxylic acid (TCA) cycle together with heterologously expressing the p4h gene from Dactylosporangium sp...
August 13, 2018: Biotechnology and Bioengineering
https://www.readbyqxmd.com/read/30101437/clinical-relevance-of-serum-antibodies-to-gd1b-in-immune-mediated-neuropathies
#2
Noor E Taams, Nicolette C Notermans, Willem-Jan R Fokkink, Anne P Tio-Gillen, Ruth Huizinga, Marco W J Schreurs, Bart C Jacobs
Antibodies to the ganglioside GD1b have been reported in various forms of immune-mediated neuropathy, but their clinical relevance for diagnosis and prognosis is unknown. We investigated the prevalence of anti-GD1b antibodies in acute and chronic immune-mediated neuropathies, and the clinical presentation and outcome in Guillain-Barré syndrome (GBS) and Miller Fisher-GBS overlap syndrome (MF-GBS). Anti-GD1b, anti-GM1 and anti-GQ1b antibodies were tested in serum of patients with GBS (N=165), MFS (N=10), MF-GBS (N=28), monoclonal gammopathy of unknown significance neuropathy (MGUS,N=101), chronic inflammatory demyelinating polyneuropathy (CIDP,N=29), paraneoplastic syndrome with anti-Hu associated neuropathy (PNS,N=11), other auto-immune diseases (AID,N=60) and healthy controls (HC,N=60)...
August 12, 2018: Journal of the Peripheral Nervous System: JPNS
https://www.readbyqxmd.com/read/30101343/a-new-pcr-assay-based-on-the-new-gene-spul_2693-for-rapid-detection-of-salmonella-enterica-subsp-enterica-serovar-gallinarum-biovars-gallinarum-and-pullorum
#3
Ying Xu, Yachen Hu, Yaxin Guo, Zihao Zhou, Dan Xiong, Chuang Meng, Qiuchun Li, Shizhong Geng, Zhiming Pan, Xinan Jiao
Salmonella enterica subsp. enterica serovar Gallinarum biovar Gallinarum (S. Gallinarum) and biovar Pullorum (S. Pullorum) are gram-negative bacteria, members of the most important infectious pathogens, and have caused common problems in the poultry industry, especially in the developing countries. O- and H-antigen specific anti-sera are commonly for slide and tube agglutination tests to identify Salmonella serovars. However, it is both labor intensive and time consuming, so there is an urgent need for a new technique for the rapid detection of the major Salmonella serovars...
August 7, 2018: Poultry Science
https://www.readbyqxmd.com/read/30100743/extracts-and-components-of-ficus-carica-leaves-suppress-survival-cell-cycle-and-migration-of-triple-negative-breast-cancer-mda-mb-231-cells
#4
Yu Zhang, Youzhong Wan, Bo Huo, Boyuan Li, Yue Jin, Xin Hu
Background: Products from Ficus carica have been used in traditional medicine to treat many diseases. This study aimed to analyze anticancer effects of extracts of F. carica leaves on the triple-negative breast cancer cell line MDA-MB-231. Materials and methods: The human breast cancer cell line MDA-MB-231 was used to evaluate effects of F. carica extracts. Effects of F. carica on cell viability were evaluated using MTT assays. Cell-cycle distribution was examined using cell-cycle analysis...
2018: OncoTargets and Therapy
https://www.readbyqxmd.com/read/30099447/emodin-and-its-combination-with-cytarabine-induce-apoptosis-in-resistant-acute-myeloid-leukemia-cells-in-vitro-and-in-vivo
#5
Yingyu Chen, Donghui Gan, Qinghua Huang, Xiaofeng Luo, Donghong Lin, Jianda Hu
BACKGROUND/AIMS: Acute myeloid leukemia (AML) remains a hematologic malignancy with poor survival and a high risk of relapse, which is mainly caused by the emergence of multidrug resistance (MDR). The identification of novel agents to improve therapeutic strategies becomes important priority for AML treatment. It has been shown that emodin has therapeutic effects on many kinds of human malignant tumors. In this study, we investigated the anti-leukemia effects of emodin alone or in combination with cytarabine (Ara-C) on multidrug-resistant AML HL-60/ADR cells and in a mouse xenograft model of human highly tumorigenic AML HL-60/H3 cells...
August 10, 2018: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/30097125/total-sesquiterpene-lactones-isolated-from-inula-helenium-l-attenuates-2-4-dinitrochlorobenzene-induced-atopic-dermatitis-like-skin-lesions-in-mice
#6
Qun Wang, Shuang Gao, Guo-Zhen Wu, Niao Yang, Xian-Peng Zu, Wen-Cai Li, Ning Xie, Rong-Rong Zhang, Chen-Wei Li, Zhen-Lin Hu, Wei-Dong Zhang
BACKGROUND: Inula helenium L. is an herb whose anti-inflammatory properties are attributed to its active components, the sesquiterpene lactones (SLs). Our previous study demonstrated that the total sesquiterpene lactones isolated from Inula helenium L. (TSL-IHL), consisting mainly of alantolactone (AL) and isoalantolactone (IAL), may have potential in the prevention and treatment of rheumatoid arthritis (RA). However, the effect of TSL-IHL on atopic dermatitis (AD) has not been studied yet...
July 15, 2018: Phytomedicine: International Journal of Phytotherapy and Phytopharmacology
https://www.readbyqxmd.com/read/30096626/integration-of-bioinformatics-and-experiments-to-identify-tp53-as-a-potential-target-in-emodin-inhibiting-diffuse-large-b-cell-lymphoma
#7
Yanxin Chen, Xuqiao Mei, Donghui Gan, Zhengjun Wu, Yanqin Cao, Minhui Lin, Na Zhang, Ting Yang, Yingyu Chen, Jianda Hu
Non-Hodgkin's Lymphoma (NHL) is a group of lymphoid malignancies with unsatisfactory treatment effect in some aggressive subtypes, including diffuse large B cell lymphoma (DLBCL). Emodin is an anthraquinone with potent anti-cancer activities. However, the molecular mechanism of Emodin repressing aggressive NHL remains to be revealed in detail. This study delineated the active mechanism of Emodin action in aggressive NHL by using bioinformatics analysis and in vitro assay. 4 Emodin's primary direct protein targets (DPT) were identified and the DPTs-associated proteins/genes were predicted...
August 6, 2018: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/30096409/interleukin-17-enhances-cardiac-ventricular-remodeling-via-activating-mapk-pathway-in-ischemic-heart-failure
#8
Shih-Lin Chang, Ya-Wen Hsiao, Yung-Nan Tsai, Shien-Fong Lin, Shuen-Hsin Liu, Yenn-Jiang Lin, Li-Wei Lo, Fa-Po Chung, Tze-Fan Chao, Yu-Feng Hu, Ta-Chuan Tuan, Jo-Nan Liao, Yu-Cheng Hsieh, Tsu-Juey Wu, Satoshi Higa, Shih-Ann Chen
BACKGROUND: We aimed to investigate the impact of interleukin (IL)-17 on ventricular remodeling and the genesis of ventricular arrhythmia (VA) in an ischemic heart failure (HF) model. The expression of the proinflammatory cytokine IL-17 is upregulated during myocardial ischemia and plays a fundamental role in postinfarct inflammation. However, the influence of IL-17 on the genesis of VA has not yet been studied. METHODS AND RESULTS: The level of inflammation and Th17 cell (CD4+ IL-17+ ) expression in the rabbit model of ischemic HF were studied by flow cytometry, quantitative polymerase chain reaction (qPCR), and enzyme-linked immunosorbent assay (ELISA)...
August 7, 2018: Journal of Molecular and Cellular Cardiology
https://www.readbyqxmd.com/read/30096387/a-novel-anti-osteoporotic-agent-that-protects-against-postmenopausal-bone-loss-by-regulating-bone-formation-and-bone-resorption
#9
Yu-Te Yang, Jia-Hong Meng, Bin Hu, Chi-Yuan Ma, Chen-Chen Zhao, Wang-Si-Yuan Teng, Jian-Qiao Hong, Si-Hao Li, Guang-Yao Jiang, Cong Wang, Chen-He Zhou, Shi-Gui Yan
AIMS: Postmenopausal osteoporosis is a bone metabolism disease that is caused by an imbalance between bone-resorbing osteoclast and bone-forming osteoblast actions. Herein, we describe the role of troxerutin (TRX), a trihydroxyethylated derivative of rutin, in ovariectomy (OVX)-induced osteoporosis and its effects on the regulation of osteoclasts and osteoblasts. MAIN METHODS: In vivo, OVX female mice were intraperitoneally injected with either saline, 50 mg/kg TRX, or 150 mg/kg TRX for 6 weeks and then sacrificed for micro-computed tomography analyses, histological analyses, and biomechanical testing...
August 7, 2018: Life Sciences
https://www.readbyqxmd.com/read/30095283/myricetin-attenuates-lps-induced-inflammation-in-raw-264-7-macrophages-and-mouse-models
#10
Wei Hou, Siyi Hu, Zhenzhong Su, Qi Wang, Guangping Meng, Tingting Guo, Jie Zhang, Peng Gao
AIM: Acute lung injury is a common clinical syndrome associated with significant morbidity. Myricetin has been demonstrated to inhibit inflammation in a variety of diseases. In this study, we aimed to investigate the protective effects of myricetin on inflammation in lipopolysaccharide-stimulated RAW 264.7 cells and lipopolysaccharide-induced lung injury model. Results/methodology: In this study, we detected the anti-inflammatory effects of myricetin by ELISA, RT-PCR and Western blot, respectively...
August 10, 2018: Future Medicinal Chemistry
https://www.readbyqxmd.com/read/30094101/bufalin-inhibits-glycolysis-induced-cell-growth-and-proliferation-through-the-suppression-of-integrin-%C3%AE-2-fak-signaling-pathway-in-ovarian-cancer
#11
Haoran Li, Shuang Hu, Yangyang Pang, Mengjiao Li, Lihua Chen, Fei Liu, Mingming Liu, Ziliang Wang, Xi Cheng
Bufalin is the major digoxin-like component of the traditional Chinese medicine Chansu and has obvious anti-tumor effect in major malignancies, but the role of bufalin in glucose metabolism in ovarian cancer remains illustrated. Here, we sought to elucidate the regulatory function of bufalin on cell glucose metabolism in ovarian cancer. The treatment of bufalin on ovarian cancer cells effectively inhibited glucose uptake and lactate production in ovarian cancer cells. The expression levels of glycolysis-related proteins, including GLUT4, LDHB and HK2, were decreased by the treatment of bufalin detected by qRT-PCR and immunoblotting...
2018: American Journal of Cancer Research
https://www.readbyqxmd.com/read/30093955/evaluation-of-the-safety-and-tolerability-of-tamoxifen-for-ischemia-incited-renal-injury-in-mice
#12
Yuxi Wang, Zhi Zhao, Weiqi Yang, Lin Li, Fengming Zhu, Guangchang Pei, Juan Yang, Han Zhu, Huzi Xu, Meng Wang, Qian Yang, Zhizhi Hu, Pengge Wang, Gang Xu, Rui Zeng, Ying Yao
Tamoxifen is used to activate tamoxifen-dependent Cre recombinase (CreER) to generate time- and tissue-specific genetically mutant mice. However, tamoxifen is also an active estrogen analogue that binds with higher affinity to estrogen receptors and exhibits anti-apoptosis, anti-inflammation, and antifibrotic properties. Renal ischemia reperfusion (I/R) injury is characterized by increased apoptosis and inflammation, so optimal utility of tamoxifen-inducible CreER genetic systems in I/R model is important. The purpose of this study was to optimize the tamoxifen dose and evaluate its safety and tolerability in the development of mouse I/R injury...
2018: American Journal of Translational Research
https://www.readbyqxmd.com/read/30093945/celastrol-aggravates-lps-induced-inflammation-and-injuries-of-liver-and-kidney-in-mice
#13
Mengqiu Wu, Weiyi Chen, Xiaowen Yu, Dan Ding, Wen Zhang, Hu Hua, Man Xu, Xia Meng, Xuejuan Zhang, Yue Zhang, Aihua Zhang, Zhanjun Jia, Songming Huang
Sepsis, a life-threatening syndrome with uncontrolled inflammatory response, causes high morbidity and mortality worldwide. Currently, satisfactory treatments on sepsis are still lacking in clinic. Thus, new therapeutic strategies are urgently required. Recently, celastrol, a pentacyclic triterpene extracted from the traditional Chinese medicine Tripterygium Wilfordi plant, attracted great interest for its properties of anti-inflammation, anti-oxidative stress, and metabolism remodeling. However, the effect of celastrol on sepsis is still unclear...
2018: American Journal of Translational Research
https://www.readbyqxmd.com/read/30092797/sotetsuflavone-inhibits-proliferation-and-induces-apoptosis-of-a549-cells-through-ros-mediated-mitochondrial-dependent-pathway
#14
Shaohui Wang, Yanlan Hu, Yu Yan, Zhekang Cheng, Tongxiang Liu
BACKGROUND: Sotetsuflavone is isolated from Cycas revoluta Thunb., which has biological activity against tumors. However, the anti-proliferative effects of sotetsuflavone on A549 cells and its mechanism are not fully elucidated. METHODS: This study investigated the mechanisms of growth inhibition, cell cycle arrest and apoptosis in non-small cell lung cancer A549 cells induced by sotetsuflavone and evaluated whether sotetsuflavone can be safely utilized by humans as therapeutic agent...
August 9, 2018: BMC Complementary and Alternative Medicine
https://www.readbyqxmd.com/read/30092635/cs4pbx6-x-cl-br-i-nanocrystals-preparation-water-triggered-transformation-behavior-and-anti-counterfeiting-application
#15
Xiaoya Yu, Linzhong Wu, Huicheng Hu, Min Chen, Yeshu Tan, Di Yang, Qi Pan, Qixuan Zhong, Thidarat Supasai, Qiao Zhang
As a promising material, Cs4PbX6 (X = Cl, Br, I) nanocrystals (NCs) have attracted much attention. However, their luminescent property is still under debate. In this work, we first systematically studied the colloidal preparation of Cs4PbX6 NCs. It is found that the critical parameter for the formation of Cs4PbX6 NCs is the ratio between Cs and Pb. Pure Cs4PbX6 NCs are non-luminescent. The luminescence property of previous reported Cs4PbX6 NCs may come from the impurity of luminescent CsPbX3 NCs. No co-existence of both Cs4PbX6 and CsPbX3 phase has been found in one single nanoparticle...
August 9, 2018: Langmuir: the ACS Journal of Surfaces and Colloids
https://www.readbyqxmd.com/read/30090962/protective-immunity-elicited-by-measles-vaccine-exerts-anti-tumor-effects-on-measles-virus-hemagglutinin-gene-modified-cancer-cells-in-a-mouse-model
#16
Yuan Qi, Kailin Xing, Lanlin Zhang, Fangyu Zhao, Ming Yao, Aiqun Hu, Xianghua Wu
PURPOSE: Measles vaccine is widely used in China to prevent the measles virus (MV) infection. People immunized with measles vaccine can obtain long-term protective immunity. Measles virus surface glycoprotein hemagglutinin (H) can also induce MV-specific immune responses. However, little is known about whether the existence of the protective immune system against MV in the host can exert anti-tumor effects and whether the MV-H gene can serve as a therapeutic gene. METHODS: We first vaccinated mice with measles vaccine, then inoculated them with MV-H protein-expressing tumor cells and observed the rate of tumor formation...
August 6, 2018: Journal of Cancer Research and Clinical Oncology
https://www.readbyqxmd.com/read/30090574/mitochondrial-toxicity-of-organic-arsenicals-membrane-permeability-transition-pore-opening-and-respiratory-dysfunction
#17
Xiao-Yang Fan, Lian Yuan, Can Wu, Yu-Jiao Liu, Feng-Lei Jiang, Yan-Jun Hu, Yi Liu
In order to clarify the mitochondrial toxicity mechanism of the organic arsenical MOPIMP (2-methoxy-4-(((4-(oxoarsanyl) phenyl) imino) methyl) phenol), research was carried out at the sub-cell level based on the previous finding that the compound MOPIMP can damage the mitochondria by triggering a burst of ROS. After investigating its influence on isolated mitochondria in vitro , it was demonstrated that a high dose of MOPIMP with short-term exposure can induce mitochondrial swelling, decrease the membrane potential, enhance the permeability of H+ and K+ , and induce membrane lipid peroxidation, indicating that it can result in an MPT process in a ROS-mediated and Ca2+ -independent manner...
March 1, 2018: Toxicology Research
https://www.readbyqxmd.com/read/30089893/the-protective-effect-of-microrna-21-in-neurons-after-spinal-cord-injury
#18
Tao Zhang, Shuangfei Ni, Zixiang Luo, Ye Lang, Jianzhong Hu, Hongbin Lu
STUDY DESIGN: Experimental animal study. OBJECTIVES: To validate the anti-apoptosis effect of microRNA-21 in neurons after spinal cord injury (SCI) and explore the mechanism. SETTING: Xiangya Hospital, Central South University, Changsha, Hunan, People's Republic of China. METHODS: In situ hybridization was used to detect the expression of miR-21 in spinal cord neurons (n = 24). In a rat contusion SCI model (n = 48), we upregulated the miR-21 level around the injured area using miR-21 lentiviral vectors and evaluated the therapeutic effect with histology and behavioural scores...
August 8, 2018: Spinal Cord
https://www.readbyqxmd.com/read/30089882/seroepidemiology-of-hepatitis-b-virus-infection-in-children-12-years-after-china-s-expanded-program-on-immunization-in-qamdo-tibet
#19
Yao Hu, Ciyang, Dunzhuyongzong, Awangluosong, Zuoqing Yang, Aqing
Although hepatitis B vaccine (HepB1 ) was first recommended through China's Expanded Program on Immunization (EPI) in 1992, Tibet was able to offer universal HepB1 vaccination starting only in early 2004. This cross-sectional study was conducted to assess, 12 years after administration of HepB1 , the seroepidemiology of hepatitis B virus (HBV) infection in children in Qamdo, Tibet. Focusing on a population of individuals ≤ 15 years of age living in Tibet, we analyzed serum samples from 261 healthy children for HBV status based on the presence of hepatitis B surface antigen (HBsAg), antibodies against HBV core antigen (anti-HBc), and antibodies against HBsAg (anti-HBs)...
August 8, 2018: Journal of Public Health Policy
https://www.readbyqxmd.com/read/30089757/carotid-intima-media-thickness-in-patients-with-ankylosing-spondylitis-a-systematic-review-and-updated-meta-analysis
#20
Yaping Yuan, Jiajia Yang, Xu Zhang, Renfang Han, Mengya Chen, Xingxing Hu, Yubo Ma, Meng Wu, Mengmeng Wang, Shengqian Xu, Faming Pan
AIM: Inflammatory arthritis (IA) diseases are relevant with subclinical atherosclerosis, but the data in ankylosing spondylitis (AS) were inconsistent. Therefore, we performed this meta-analysis to explore the relationship between the marker of subclinical atherosclerosis (carotid intima-media thickness (IMT)) and AS. METHODS: We performed a systematic literature review using PubMed, Web of Science, Chinese National Knowledge Infrastructure (CNKI) and Chinese Biomedical Database (CBM) databases up to March 2018...
August 8, 2018: Journal of Atherosclerosis and Thrombosis
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