Serang Bong, Chung Bin Park, Shin-Gyu Cho, Jaeyoung Bae, Natalia Diyah Hapsari, Xuelin Jin, Sujung Heo, Ji-Eun Lee, Kaori Hashiya, Toshikazu Bando, Hiroshi Sugiyama, Kwang-Hwan Jung, Bong June Sung, Kyubong Jo
In this study, we specifically visualized DNA molecules at their AT base pairs after in vitro phage ejection. Our AT-specific visualization revealed that either end of the DNA molecule could be ejected first with a nearly 50% probability. This observation challenges the generally accepted theory of Last In First Out (LIFO), which states that the end of the phage λ DNA that enters the capsid last during phage packaging is the first to be ejected, and that both ends of the DNA are unable to move within the extremely condensed phage capsid...
May 9, 2023: Nucleic Acids Research