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head and neck stem cell

M Janaki Ramaiah, S Vaishnave
BMI-1 (B-lymphoma Mo-MLV insertion region 1) is a key protein partner in polycomb repressive complex 1 (PRC1) that helps in maintaining the integrity of the complex. It is also a key player in ubiquitination of histone H2A which affects gene expression pattern involved in various cellular processes such as cell proliferation, growth, DNA repair, apoptosis and senescence. In many cancers, Overexpression of BMI1correlates with advanced stages of disease, aggressive clinicopathological behavior, poor prognosis resistance to radiation and chemotherapy...
August 7, 2018: Gene
Chao Yang, Hongwei Tang, Limei Wang, Renjun Peng, Fan Bai, Yajun Shan, Zuyin Yu, Pingkun Zhou, Yuwen Cong
PURPOSE: Oral mucositis is one of the most prevalent side effects in patients undergoing radiotherapy for head and neck cancers. Current therapeutic agents such as palifermin (rhKGF) and amifostine do not efficiently or fully prevent mucositis. Dimethyl sulfoxide (DMSO), a free-radical scavenger, has shown therapeutic benefits in many preclinical and clinical studies. This study aimed to investigate the efficacy of DMSO in a clinically relevant mouse model of acute, radiation-induced oral mucositis...
August 6, 2018: International Journal of Radiation Oncology, Biology, Physics
Raissa Borges Curtarelli, Jussara Maria Gonçalves, Luciane Geanini Pena Dos Santos, Maria Gorete Savi, Jacques Eduardo Nör, Luis André Mendonça Mezzomo, Mabel Mariela Rodríguez Cordeiro
Malignant neoplasms may be composed of several cell groups, including cancer stem cells (CSC). These cells have been related with the capacity of metastasis, relapse and resistance to multiple drugs during chemotherapy. This study aims to identify CSC biomarkers and their expression pattern in human head and neck carcinomas. This study was conducted following the PRISMA checklist. The search for articles was carried out in five databases (PubMed, Scopus, Web of Science, Lilacs and Scielo). The articles found were selected in two phases: 1) reading the titles and / or abstract and 2) reading the full text...
August 3, 2018: Stem Cell Reviews
Lorena Salazar-García, Mario Pérez-Sayáns, Abel García-García, Ángel Carracedo, Raquel Cruz, Antonio Lozano, Beatriz Sobrino, Francisco Barros
BACKGROUND: Head and neck squamous cell carcinoma (HNSCC) is a global public health problem, which increases in prevalence every year. Evidence brings up that small populations of stem cells, the cancer stem cells (CSC), are responsible for tumour initiation, tumorigenesis, progression, and metastasis. These cells play a crucial role in cancer development and treatment response, but the origin of CSCs remains unclear. In this research, we have sequenced the entire exome of the three different groups of cells of primary squamous cell carcinoma tumour from different patients, with the aim to understand their main genetic changes and to elucidate the possible origin of tumour cells and tumour stem cells...
August 1, 2018: Journal of Oral Pathology & Medicine
Celso Muller Bandeira, Adriana Ávila de Almeida, Celina Faig Lima Carta, Alaor Aparecido Almeida, Fellipe Augusto Tocchini de Figueiredo, Valeria Cristina Sandrim, Antonio José Gonçalves, Janete Dias Almeida
Heavy metals intoxication is known to be risk factors for various diseases, including cancer. These metals may be presented in food and soil as well as in leaf and tobacco smoke. The aim of this study was to correlate the exposure to heavy metals stemming from tobacco and head and neck squamous cell carcinoma carcinogenesis. Analysis of lead, copper, manganese, arsenic, chromium, and cadmium by atomic absorption spectrophotometry was performed in whole blood samples from 91 patients: 68 smokers with oral cavity, pharynx, or laryngeal cancer; 8 non-smokers with oral or larynx cancer; and 15 non-cancer smokers with tobacco-related diseases (control group)...
July 28, 2018: Environmental Science and Pollution Research International
Chun-Chia Cheng, Hsin-Chi Lin, Kaun-Jer Tsai, Ya-Wen Chiang, Ken-Hong Lim, Caleb Gon-Shen Chen, Ying-Wen Su, Cheng-Liang Peng, Ai-Sheng Ho, Ling Huang, Yu-Cheng Chang, Huan-Chau Lin, Jungshan Chang, Yi-Fang Chang
The epidermal growth factor (EGF) receptor (EGFR) overexpressed in many cancers, including lung and head and neck cancers, and is involved in cancer cell progression and survival. PD-L1, increases in tumor cells to evade and inhibit CD8+ T cells, is a clinical immunotherapeutic target. This study investigated the molecular mechanism of EGF on regulating PD-L1 in EGFR-positive cancers and determined potential agents to reduce PD-L1 expression. RNA sequencing (RNAseq) and bioinformatics analysis were performed to determine potential driver genes that regulate PD-L1 in tumor cells-derived tumorspheres which mimicking cancer stem cells...
July 23, 2018: Molecular Carcinogenesis
Vinay K Kartha, Khalid A Alamoud, Khikmet Sadykov, Bach-Cuc Nguyen, Fabrice Laroche, Hui Feng, Jina Lee, Sara I Pai, Xaralabos Varelas, Ann Marie Egloff, Jennifer E Snyder-Cappione, Anna C Belkina, Manish V Bais, Stefano Monti, Maria A Kukuruzinska
BACKGROUND: Head and neck squamous cell carcinoma (HNSCC) is an aggressive malignancy characterized by tumor heterogeneity, locoregional metastases, and resistance to existing treatments. Although a number of genomic and molecular alterations associated with HNSCC have been identified, they have had limited impact on the clinical management of this disease. To date, few targeted therapies are available for HNSCC, and only a small fraction of patients have benefited from these treatments...
July 20, 2018: Genome Medicine
Y Xie, J-Z Hu, Z-Y Shi
OBJECTIVE: We aimed to investigate whether miR-181d may be involved in steroid-induced osteonecrosis of the femoral head (ONFH) and its underlying mechanism. PATIENTS AND METHODS: Quantitative reverse transcriptase-polymerase chain reaction (qRT-PCR) was used to detect the expression of miR-181d in bone marrow of 5 cases of steroid-induced femoral head necrosis and 5 cases of femoral head necrosis secondary to femoral neck fractures. Marrow-derived mesenchymal stem cells (MSCs) were obtained from bone marrow samples and identified...
July 2018: European Review for Medical and Pharmacological Sciences
Jin Chen, Guiming Fu, Yibo Chen, Guiquan Zhu, Zhaohui Wang
Postoperative radiotherapy or concurrent chemoradiotherapy are routine clinical options for the treatment of head and neck squamous cell carcinoma (HNSCC). However, the benefit of adding chemotherapy to radiotherapy is contested. The present study aimed to develop a gene signature to predict the clinical benefit of postoperative chemoradiotherapy using public data from The Cancer Genome Atlas. A 22-gene signature was established, which demonstrated the best predictive value. Patients were separated into low-score and high-score subgroups based on the expression score of the 22-gene signature...
August 2018: Oncology Letters
Danielle Wu, Patricia Chapela, Mary C Farach-Carson
This chapter focuses on the culture of primary human cells from the salivary glands, typically parotid but also submandibular, where specialized acinar cells produce most of the components found in saliva and the intercalated ducts followed by striated ducts transport saliva to the oral cavity. Compared to many other epithelial cells, the zymogen-filled salivary acinar cells are very fragile, hence specialized techniques are needed to isolate and culture them. To reestablish the function of implantable 3D reassembled glands using tissue engineering approaches, it is critical to culture these cells in human-based matrices that permit them to move, reassemble, interconnect, and establish proper polarity by producing a basement membrane...
2018: Methods in Molecular Biology
Chih-Yuan Fang, Pei-Yin Chen, Dennis Chun-Yu Ho, Lo-Lin Tsai, Pei-Ling Hsieh, Ming-Yi Lu, Cheng-Chia Yu, Chuan-Hang Yu
5-Aminolevulinic acid-mediated photodynamic therapy (ALA-PDT) has been used in the treatment of various precancerous and malignant lesions. Our previous work has demonstrated that ALA-PDT possesses the potential to serve as an adjuvant therapy against head and neck cancer via eliminating the cancer stem cells (CSCs) property. This study aimed to further investigate the possible molecular mechanism underlying the effect of ALA-PDT. Our results revealed that ALA-PDT upregulated the expression of microRNA-145 (miR-145) in two oral cancer cell lines...
August 2018: Journal of the Formosan Medical Association, Taiwan Yi Zhi
Xu Qian, Xiaobo Nie, Wenhao Yao, Konrad Klinghammer, Holger Sudhoff, Andreas M Kaufmann, Andreas E Albers
One of the greatest challenges in systemic treatment of head and neck squamous cell carcinoma (HNSCC) is a small tumor cell population, namely, cancer stem-like cells (CSC). CSC can regenerate and maintain a heterogenic tumor by their self-renewal capacity. Their potential ability to be more resistant to and survival after chemo- and radiation therapy was also identified. Further studies have shown that reactive oxygen species (ROS) contribute to this CSC-associated resistance. In this review, we focus on the current knowledge of HNSCC-CSC, with regard to ROS as a possible and novel therapeutic approach in targeting CSC...
June 20, 2018: Seminars in Cancer Biology
Aiwen Yu, Yu Wang, Yue Bian, Lisha Chen, Junfu Guo, Wei Shen, Danqi Chen, Shanshan Liu, Xiuju Sun
It has been shown that nuclear expression of S100A4 is significantly correlated with increased metastasis and reduced survival in patients with gastric cancer and many other cancers. However, the factors which could influence the nuclear contents of S100A4 in cancer cells are not clear. It has also been reported that Interleukin-1β (IL-1β) promotes the nuclear translocation of S100A4 in chondrocytes. Previous studies have shown that IL-1β promotes the stemness of colon cancer cells, and S100A4 is also involved in maintaining cancer-initiating cells in head and neck cancers...
June 22, 2018: Journal of Cellular Biochemistry
Johannes Taeger, Agmal Scherzad, Doris Feineis, Raina Seupel, Rudolf Hagen, Norbert Kleinsasser, Stephan Hackenberg
Despite partial advances in therapy for patients suffering from head and neck squamous cell carcinomas (HNSCC), prognosis still remains poor with minimal improvement in survival for over the last several decades. Some agents found are known to cause cancer cell death in vitro by promoting cellular reactive oxygen species (ROS) accumulation. This is particularly of interest as some cancer cells are more sensitive to ROS than normal cells. It could be shown that the novel polyphenol conjugate (E)-3-(3',5'-Dimethoxyphenyl)-1-(2'-methoxyphenyl)prop-2-en-1-one (DPP-23) offers antitumor effects by the selective generation of ROS without an indication of toxicity in normal tissues in vitro and in vivo ...
July 2018: Oncology Letters
Ola M Ezzatt, Iman M Helmy
OBJECTIVES: Oral lichen plans (OLP) is a potentially malignant inflammatory mucocutaneous disease. CD133 is an investigated surface marker for cancer stem-like cells (CSCs) that may be involved in tumor initiation in head and neck carcinomas. We compared short-term clinical effectiveness of topical pimecrolimus as selective inflammatory cytokine release inhibitor with betamethasone cream for erosive/atrophic OLP and investigated the influence of this therapy on CD133 expression. MATERIAL AND METHODS: Thirty patients were randomly assigned into two equal groups to receive topical pimecrolimus (group I) or betamethasone (group II) four times daily for 4 weeks...
June 16, 2018: Clinical Oral Investigations
L Tsiklauri, J Werner, M Kampschulte, K W Frommer, L Berninger, M Irrgang, K Glenske, D Hose, T El Khassawna, J Pons-Kühnemann, S Rehart, S Wenisch, U Müller-Ladner, E Neumann
OBJECTIVES: Age-related bone loss is associated with bone marrow adiposity. Adipokines (e.g., visfatin, resistin, leptin) are adipocyte-derived factors with immunomodulatory properties and might influence differentiation of bone marrow-derived mesenchymal stem cells (MSC) in osteoarthritis (OA) and osteoporosis (OP). Thus, the presence of adipokines and MMPs in bone marrow and their effects on MSC differentiation were analyzed. METHODS: MSC and Ribonucleic acid (RNA) were isolated from femoral heads after hip replacement surgery of OA or osteoporotic femoral neck fracture (FF) patients...
June 14, 2018: Osteoarthritis and Cartilage
Hyun Nam, Ji-Hye Kim, Ji-Yoon Hwang, Gee-Hye Kim, Jae-Won Kim, Mi Jang, Jong-Ho Lee, Kyungpyo Park, Gene Lee
Patients with head and neck cancer are treated with therapeutic irradiation, which can result in irreversible salivary gland dysfunction. Because there is no complete cure for such patients, stem cell therapy is an emerging alternative for functional restoration of salivary glands. In this study, we investigated in vitro characteristics of primarily isolated epithelial cells from human salivary gland (Epi-SGs) and in vivo formation of acini-like structures by Epi-SGs. Primarily isolated Epi-SGs showed typical epithelial cell-like morphology and expressed E-cadherin but not N-cadherin...
June 2018: Molecules and Cells
Amrendra Mishra, Harshini Sriram, Pinal Chandarana, Vivek Tanavde, Rekha V Kumar, Ashok Gopinath, Raman Govindarajan, S Ramaswamy, Subhashini Sadasivam
The goal of this study was to isolate cancer stem-like cells marked by high expression of CD44, a putative cancer stem cell marker, from primary oral squamous cell carcinomas and identify distinctive gene expression patterns in these cells. From 1 October 2013 to 4 September 2015, 76 stage III-IV primary oral squamous cell carcinoma of the gingivobuccal sulcus were resected. In all, 13 tumours were analysed by immunohistochemistry to visualise CD44-expressing cells. Expression of CD44 within The Cancer Genome Atlas-Head and Neck Squamous Cell Carcinoma RNA-sequencing data was also assessed...
May 2018: Tumour Biology: the Journal of the International Society for Oncodevelopmental Biology and Medicine
Sarah C McDermott, Christie Rodriguez-Ramirez, Sean P McDermott, Max S Wicha, Jacques E Nör
Patients with recurrent or metastatic head and neck squamous cell carcinoma (HNSCC) have poor prognosis with less than 1-year median survival. Platinum-based chemotherapy remains the first-line treatment for HNSCC. The cancer stem cell (CSC) hypothesis postulates that tumors are maintained by a self-renewing CSC population that is also capable of differentiating into non-self renewing cell populations that constitute the bulk of the tumor. A small population of CSC exists within HNSCC that are relatively resistant to chemotherapy and clinically predicted to contribute to tumor recurrence...
May 18, 2018: Oncotarget
Farnaz Mohajertehran, Amirhossein Sahebkar, Reza Zare, Nooshin Mohtasham
Oral squamous cell carcinoma (OSCC) is the most common type of head and neck cancer. Epithelial dysplasia is often initiated in the cells and cell nuclei adjacent to the epithelial cell membrane. Reduced cell-cell adhesions enable cancer cells to detach from the tumor and disseminate to other organs. The mutations in epithelial dysplasia markers such as E-cadherin and epithelial cell adhesion molecules (CD326) can lead to proliferation, growth and survival of the tumor cells and persistence of numerous malignancies that play a key role in epithelial dysplasia of OSCC...
May 24, 2018: Journal of Cellular Physiology
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