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https://www.readbyqxmd.com/read/30310944/quantitative-mapping-of-specific-proteins-in-biological-tissues-by-laser-ablation-icp-ms-using-exogenous-labels-aspects-to-be-considered
#1
REVIEW
María Cruz-Alonso, Ana Lores-Padín, Eva Valencia, Héctor González-Iglesias, Beatriz Fernández, Rosario Pereiro
Laser ablation (LA) coupled with inductively coupled plasma mass spectrometry (ICP-MS) is a versatile tool for direct trace elemental and isotopic analysis of solids. The development of new strategies for quantitative elemental mapping of biological tissues is one of the growing research areas in LA-ICP-MS. On the other hand, the latest advances are related to obtaining not only the elemental distribution of heteroatoms but also molecular information. In this vein, mapping of specific proteins in biological tissues can be done with LA-ICP-MS by use of metal-labelled immunoprobes...
October 12, 2018: Analytical and Bioanalytical Chemistry
https://www.readbyqxmd.com/read/30309755/tumescence-anesthesia-solution-assisted-laser-ablation-treatment-of-lower-limb-varicose-veins-the-effect-of-temperature-of-the-tumescence-anesthesia-solution-on-intraoperative-and-postoperative-pain-clinical-observations-and-comprehensive-nursing-care
#2
Li-Hua Luo, Zhu Chen, Li-Nan Hu, Cong Ma, En-Hua Xiao
PURPOSE: To investigate the effect of cold and room temperature tumescence anesthesia solution (TAS) on the treatment of lower limb varicose veins via endovenous laser ablation. DESIGN: On the basis of the TAS temperature, patients were divided into two groups: group A (n = 26) received room temperature (24°C) TAS, and group B (n = 25) received cold (4°C) TAS. METHODS: A numerical rating scale was used to evaluate pain. Perioperative and intraoperative nursing care and clinical observations were performed following a generalized standard...
October 8, 2018: Journal of Perianesthesia Nursing: Official Journal of the American Society of PeriAnesthesia Nurses
https://www.readbyqxmd.com/read/30297721/visualising-peripheral-arterioles-and-venules-through-high-resolution-and-large-area-photoacoustic-imaging
#3
Yoshiaki Matsumoto, Yasufumi Asao, Hiroyuki Sekiguchi, Aya Yoshikawa, Tomoko Ishii, Ken-Ichi Nagae, Shuichi Kobayashi, Itaru Tsuge, Susumu Saito, Masahiro Takada, Yoshihiro Ishida, Masako Kataoka, Takaki Sakurai, Takayuki Yagi, Kenji Kabashima, Shigehiko Suzuki, Kaori Togashi, Tsuyoshi Shiina, Masakazu Toi
Photoacoustic (PA) imaging (PAI) has been shown to be a promising tool for non-invasive blood vessel imaging. A PAI system comprising a hemispherical detector array (HDA) has been reported previously as a method providing high morphological reproducibility. However, further improvements in diagnostic capability will require improving the image quality of PAI and fusing functional and morphological imaging. Our newly developed PAI system prototype not only enhances the PA image resolution but also acquires ultrasonic (US) B-mode images at continuous positions in the same coordinate axes...
October 8, 2018: Scientific Reports
https://www.readbyqxmd.com/read/30267092/pathophysiological-role-of-vegf-on-retinal-edema-and-nonperfused-areas-in-mouse-eyes-with-retinal-vein-occlusion
#4
Anri Nishinaka, Yuki Inoue, Shinichiro Fuma, Yoshifumi Hida, Shinsuke Nakamura, Masamitsu Shimazawa, Hideaki Hara
Purpose: To determine the relationship between retinal morphologic changes and molecules involved in the changes after anti-VEGF treatment in the retina of retinal vein occlusion (RVO) murine model. Methods: The studies were performed on murine RVO model created by laser irradiation of retinal veins. The site of VEGF expression was determined by immunostaining, and the retinal thickness was measured in the images obtained by optical coherence tomography. The levels of VEGF-related and inflammatory factors after an intravitreal injection of anti-VEGF antibody immediately or 7 days after laser irradiation were determined by Western blotting...
September 4, 2018: Investigative Ophthalmology & Visual Science
https://www.readbyqxmd.com/read/30263121/cost-effectiveness-analysis-of-five-procedures-for-great-saphenous-vein-reflux-in-a-norwegian-healthcare-setting-or-societal-setting
#5
Eivind Inderhaug, Carl-Henrik Schelp, Inge Glambek, Ivar S Kristiansen
Objective: The aim of this work was to estimate cost-effectiveness of five common procedures for varicose vein surgery ( high ligation and stripping, radiofrequency ablation, endovenous laser ablation, steam vein sclerosis and cyanoacrylate glue ) in a Norwegian setting from both a societal and a healthcare payer perspective. Design: Cost-effectiveness analysis using decision tree modelling. Methods: A structured literature search was conducted to estimate the clinical effectiveness and the rate of complications in the five methods...
2018: SAGE Open Medicine
https://www.readbyqxmd.com/read/30259004/changes-in-blood-flow-velocity-and-thickness-of-the-choroid-in-a-patient-with-leukemic-retinopathy
#6
Akari Takita, Yuki Hashimoto, Wataru Saito, Satoru Kase, Susumu Ishida
Purpose: Choroidal circulation hemodynamics in eyes with leukemia has not been quantitatively examined yet. We quantitatively examined changes in choroidal blood flow velocity and choroidal thickness at the macula by using laser speckle flowgraphy (LSFG) and enhanced depth imaging optical coherence tomography (EDI-OCT) in a patient with leukemic retinopathy. Observations: A 15-year-old boy presented with sudden central vision loss of his right eye. The patient's best-corrected visual acuity was 0...
December 2018: American Journal of Ophthalmology Case Reports
https://www.readbyqxmd.com/read/30238444/a-randomized-trial-of-early-endovenous-ablation-in-venous-ulceration-a-critical-appraisal
#7
K T Weiß, F Zeman, S Schreml
Manjit S. Gohel et al. (N Engl J Med 2018; 378: 2105-2114) aimed to compare early endovenous ablation vs. deferred endovenous ablation of superficial venous reflux regarding time to healing of venous leg ulcers, rate of healing at 24 weeks, recurrence rate, ulcer-free time, and health-related quality of life. SETTING AND DESIGN: This multicentre, parallel-group (ratio 1:1), randomized controlled trial was conducted in a vascular surgery department setting at 20 participating centres across the United Kingdom...
September 20, 2018: British Journal of Dermatology
https://www.readbyqxmd.com/read/30233719/reduced-expression-of-microrna-199a-3p-is-associated-with-vascular-endothelial-cell-injury-induced-by-type-2-diabetes-mellitus
#8
Hui Wang, Zhengxia Wang, Qingbin Tang
The aim of the present study was to investigate the function and mechanism of action of microRNA (miRNA or miR)-199a-3p in vascular endothelial cell injury induced by type 2 diabetes mellitus (T2DM). A total of 36 patients with T2DM (26 males and 10 females; mean age, 52.5±7.0 years) and 20 healthy subjects (10 males and 10 females; mean age, 55.6±4.5 years) were included in the present study. Peripheral blood samples were obtained from all participants and total RNA was extracted Reverse transcription-quantitative polymerase chain reaction was performed to determine the expression of miR-199a-3p...
October 2018: Experimental and Therapeutic Medicine
https://www.readbyqxmd.com/read/30231633/-laser-surgery-on-varicose-veins
#9
Imre Bihari, George Ayoub, Szilvia Bokros, Péter Bihari
INTRODUCTION: With the development of medicine, technical inventions have been intoduced into the therapy of varicose veins, such as ultrasound examination and laser treatment. PATIENTS AND METHODS: Over 11 years 1729 laser surgery were performed on varicose veins. Junctions of saphenous veins, saphenous stems and perforator veins were treated with lasers, but reticular and spider veins were treated with other methods. The most important elements of laser surgery are the introduction of the laser fibre into the lumen of the vein and delivering the laser energy...
September 2018: Magyar Sebészet
https://www.readbyqxmd.com/read/30227790/a-practical-approach-to-tumescent-local-anaesthesia-in-ambulatory-endovenous-thermal-ablation
#10
Isaac K Nyamekye
Background Thermal ablation, usually performed with tumescent local anaesthesia (TLA), is the preferred method for varicose veins treatment. Tumescent local anaesthesia is always cited; however, little detail of the procedure is presented in publications. This retrospective audit of clinical tumescent local anaesthesia practice aims to provide detailed information on an important aspect of endovenous practice. Methods Patients who underwent three types of endothermal treatment (Venefit, Radiofrequency Induce Thermal Therapy and Endovenous Laser Ablation) to a single saphenous trunk using tumescent local anaesthesia were assessed...
September 18, 2018: Phlebology
https://www.readbyqxmd.com/read/30222814/repeatability-of-split-spectrum-amplitude-decorrelation-angiography-to-assess-capillary-perfusion-density-within-optical-coherence-tomography
#11
Felipe F Conti, Jason M Young, Fabiana Q Silva, Eduardo B Rodrigues, Rishi P Singh
BACKGROUND AND OBJECTIVE: To evaluate the repeatability of retinal thickness and vascular density measurements using split-spectrum amplitude-decorrelation angiography (SSADA) with optical coherence tomography (OCT). PATIENTS AND METHODS: Forty patients were divided into seven categories according to their diagnosis: no retinopathy (control), retinal vein occlusion, diabetes with no retinopathy, diabetes with retinopathy, non-exudative age-related macular degeneration (AMD), exudative AMD, and epiretinal membrane...
September 1, 2018: Ophthalmic Surgery, Lasers & Imaging Retina
https://www.readbyqxmd.com/read/30222802/secondary-ocular-hypertension-and-the-risk-of-glaucoma-surgery-after-dexamethasone-intravitreal-implant-in-routine-clinical-practice
#12
Kornwipa Hemarat, Jacquelyn D Kemmer, Travis C Porco, Alexander M Eaton, Rahul N Khurana, Jay M Stewart
BACKGROUND AND OBJECTIVES: To determine the rate of ocular hypertension (OHT) after dexamethasone intravitreal implant in routine clinical practice and identify patient characteristics associated with a risk for glaucoma surgery. PATIENTS AND METHODS: The charts of 260 eyes from 221 patients with diabetic macular edema, retinal vein occlusion, uveitis, and macular edema secondary to various causes treated with one or more dexamethasone implants were reviewed. Intraocular pressure (IOP), medications, and glaucoma interventions were collected before and after implantation...
September 1, 2018: Ophthalmic Surgery, Lasers & Imaging Retina
https://www.readbyqxmd.com/read/30210605/effects-of-hepatic-blood-inflow-on-liver-ultrastructure-and-regeneration-after-extensive-liver-resection-in-rats-with-cirrhosis
#13
Wang-Xun Jin, Bing Wang, Yun-Li Zhang, Ruizeng Dong, Xin-Bao Wang, Jian-Min Guo, Sunfu Fan, Bingqi Yu
The aim of the present study was to investigate the effects of hepatic blood inflow on liver function, liver ultrastructure and the regeneration of future liver remnant (FLR) following major hepatectomy in rats with liver cirrhosis. A rat model of cirrhosis was established through intraperitoneal injection of carbon tetrachloride for 8 consecutive weeks. Extensive liver resection and different blood inflow models by portal vein (PV) and/or hepatic artery (HA) stenosis were conducted on the cirrhosis rats. Animal models were constructed as follows: Control (group A), low-flow PV + high-flow HA (group B), low-flow PV + low-flow HA (group C), high-flow PV + high-flow HA (group D) and high-flow PV + low-flow HA (group E)...
September 2018: Experimental and Therapeutic Medicine
https://www.readbyqxmd.com/read/30199878/carotid-jugular-fistula-model-to-study-systemic-effects-and-fistula-related-microcirculatory-changes
#14
Souleiman Ghanem, Bence Tanczos, Adam Deak, Laszlo Bidiga, Norbert Nemeth
BACKGROUND: Arteriovenous fistulae impair the distal circulation, but their effects at the microcirculatory level are not well understood. This study presents the carotid-jugular fistula (CJF) as a model to evaluate fistula-related microcirculatory and systemic changes. MATERIALS AND METHODS: Female Wistar rats were anesthetized and divided into a fistula group (FG, n = 10) and a sham group (SG, n = 6). End-to-end anastomosis was performed between the right carotid artery and the jugular vein in the FG...
September 10, 2018: Journal of Vascular Research
https://www.readbyqxmd.com/read/30193551/initial-outcomes-of-endovenous-laser-ablation-with-1940-nm-diode-laser-in-the-treatment-of-incompetent-saphenous-veins
#15
Insoo Park
Objectives To investigate the initial outcomes of 1940 nm diode laser in the treatment of incompetent saphenous veins. Methods This was a prospective observational study. We treated 89 patients with 160 incompetent saphenous veins using a 1940 nm diode laser and bare fiber. The laser's power was set to 4.5 W with a mean linear endovenous energy density of 50.4 J/cm. Results The one-month closure rate was 100%. The post-procedural pain score at 6 h, 1 day, 10 days, and 1 month was 0.85 ± 1.04, 0.65 ± 1...
September 7, 2018: Vascular
https://www.readbyqxmd.com/read/30186418/preserving-hepatic-artery-flow-during-portal-triad-blood-occlusion-improves-regeneration-of-the-remnant-liver-in-rats-with-obstructive-jaundice-following-partial-hepatectomy
#16
Zhe Kong, Jian-Jun Hu, Xin-Lan Ge, Ke Pan, Chong-Hui Li, Jia-Hong Dong
In certain cases, major hepatectomy is essential and inevitable in patients with hilar cholangiocarcinoma and obstructive jaundice (OJ). The current study was designed to evaluate effects of a novel method of portal blood occlusion, where the portal vein was occluded (OPV) and the hepatic artery flow was preserved in rats with OJ that underwent partial hepatectomy. OJ was induced in rats by ligation of the common bile duct for 7 days. Subsequently, OJ rats underwent hepatectomy removing 76% of the liver following occlusion of the portal triad (OPT), OPV or without portal blood occlusion...
September 2018: Experimental and Therapeutic Medicine
https://www.readbyqxmd.com/read/30166236/-profile-of-neovascular-glaucoma-in-senegalese-patients
#17
M N Ndiaye-Sow, S M Seck, M Dieng, M Diakhaté-Diouf, G A Agboton, N N Gueye
OBJECTIVE: To describe the etiologies, clinical presentation and therapeutic management of neovascular glaucoma (NVG) in Senegalese patients. PATIENTS AND METHODS: We retrospectively studied the records of patients followed for NVG between 1993 and 2016. We included eyes with Iridis rubeosis with or without ocular hypertension. We recorded age, sex, medical and ophthalmologic history, results of the eye examination, treatment modalities broken up into hospitalisation, procedural interventions and number of antiglaucoma medications prescribed...
September 2018: Journal Français D'ophtalmologie
https://www.readbyqxmd.com/read/30139330/advanced-glaucoma-secondary-to-bilateral-idiopathic-dilated-episcleral-veins-a-case-report
#18
Xin Rong, Mei Li
BACKGROUND: Idiopathic elevated episcleral venous pressure (IEEVP) or idiopathic dilated episcleral veins (IDEV) is a rare abnormality, and thus therapeutic treatment for this condition rarely is discussed. We report a case of a patient with bilateral glaucoma secondary to IDEV for whom intraocular pressures (IOPs) were controlled successfully by trabeculectomy. CASE PRESENTATION: A 50 year-old female with a complaint of persistent red eyes for over 30 years, presented with numerous tortuous and engorged episcleral vessels in both eyes (OU), open angles OU with spontaneous blood in Schlemm's canal 360 degrees bilaterally...
August 23, 2018: BMC Ophthalmology
https://www.readbyqxmd.com/read/30139028/-preclinical-application-of-mr-and-fluorescent-dual-modality-imaging-combined-with-photothermal-therapy-in-her-2-positive-breast-cancer
#19
X J Luo, J Li, C W Zhou
Objective: To construct superparamagnetic iron oxide nanoparticles (SPIONs) coated on trastuzumab and indocyanine green (ICG) and then investigate whether the coated nanoparticles (NPs) targeted to human epidermal growth factor receptor-2 (HER-2) receptors on breast cancer cells in vitro and in vivo . Methods: The Fe(3)O(4)-trastuzumab-ICG NPs were constructed. And a series of characteristics of the NPs were evaluated. The uptake ability of SK-BR-3, a HER-2 positive breast cancer cell, was observed by transmission electron microscopy...
August 23, 2018: Zhonghua Zhong Liu za Zhi [Chinese Journal of Oncology]
https://www.readbyqxmd.com/read/30137881/imaging-of-cortical-oxygen-tension-and-blood-flow-following-targeted-photothrombotic-stroke
#20
Colin T Sullender, Andrew E Mark, Taylor A Clark, Tatiana V Esipova, Sergei A Vinogradov, Theresa A Jones, Andrew K Dunn
We present a dual-modality imaging system combining laser speckle contrast imaging and oxygen-dependent quenching of phosphorescence to simultaneously map cortical blood flow and oxygen tension ( <mml:math xmlns:mml="http://www.w3.org/1998/Math/MathML"> <mml:mrow> <mml:msub> <mml:mi>pO</mml:mi> <mml:mn>2</mml:mn> </mml:msub> </mml:mrow> </mml:math> ) in mice. Phosphorescence signal localization is achieved through the use of a digital micromirror device (DMD) that allows for selective excitation of arbitrary regions of interest...
July 2018: Neurophotonics
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