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Younghoon Jang, Aaron Broun, Chaochen Wang, Young-Kwon Park, Lenan Zhuang, Ji-Eun Lee, Eugene Froimchuk, Chengyu Liu, Kai Ge
Histone 3 lysine 4 (H3K4) methyltransferases MLL3 and MLL4 (MLL3/4) are required for enhancer activation during cell differentiation, though the mechanism is incompletely understood. We have attempted to address this issue by generating two mouse lines: one expressing H3.3K4M, a lysine-4-to-methionine (K4M) mutation of histone H3.3 that inhibits H3K4 methylation, and the other carrying conditional double knockout of MLL3/4 enzymatic SET domain. Expression of H3.3K4M in lineage-specific precursor cells depletes H3K4 methylation and impairs adipose tissue and muscle development...
October 18, 2018: Nucleic Acids Research
Xue-Liang Zhou, Rong-Rong Zhu, Xia Wu, Hua Xu, Yun-Yun Li, Qi-Rong Xu, Sheng Liu, Huang Huang, Xinping Xu, Li Wan, Qi-Cai Wu, Ji-Chun Liu
Histone lysine methylation plays an important role in the regulation of ventricular remodelling. NSD2 is involved in many types of tumours through enhancing H3K36me2 expression. However, the role of NSD2 in the regulation of histone lysine methylation during ventricular remodelling remains unclear. In this study, we established cardiac hypertrophy model in C57BL/6 mice by transverse aortic constriction and found that histone lysine methylation participated in ventricular remodelling regulation via the up-regulation of H3K27me2 and H3K36me2 expression...
October 18, 2018: Journal of Cellular and Molecular Medicine
Alejandro Medrano-Fernández, Jose M Delgado-Garcia, Beatriz Del Blanco, Marián Llinares, Raudel Sánchez-Campusano, Román Olivares, Agnès Gruart, Angel Barco
The development of inhibitory circuits depends on the action of a network of transcription factors and epigenetic regulators that are critical for interneuron specification and differentiation. Although the identity of many of these transcription factors is well established, much less is known about the specific contribution of the chromatin-modifying enzymes that sculpt the interneuron epigenome. Here, we generated a mouse model in which the lysine acetyltransferase CBP is specifically removed from neural progenitors at the median ganglionic eminence (MGE), the structure where the most abundant types of cortical interneurons are born...
October 17, 2018: Molecular Neurobiology
Paresh Shrimali, Mathew Peter, Archana Singh, Neha Dalal, Suresh Dakave, Shubhada V Chiplunkar, Prakriti Tayalia
For diseases related to genetic disorders or cancer, many cellular therapies rely on the ex vivo modification of cells for attaining a desired therapeutic effect. The efficacy of such therapies involving the genetic modification of cells relies on the extent of gene expression and subsequent persistence of modified cells when infused into the patient's body. In situ gene delivery implies the manipulation of cells in their in vivo niche such that the effectiveness can be improved by minimizing post manipulation effects like cell death, lack of persistence, etc...
October 18, 2018: Biomaterials Science
Dominique Raze, Claudie Verwaerde, Gaspard Deloison, Elisabeth Werkmeister, Baptiste Coupin, Marc Loyens, Priscille Brodin, Carine Rouanet, Camille Locht
The heparin-binding hemagglutinin adhesin (HBHA) is an important virulence factor of Mycobacterium tuberculosis . It is a surface-displayed protein that serves as an adhesin for non-phagocytic cells and is involved in extra-pulmonary dissemination of the tubercle bacillus. It is also an important latency antigen useful for the diagnosis of latently M. tuberculosis -infected individuals. Using fluorescence time-lapse microscopy on mycobacteria that produce HBHA-green fluorescent protein chimera, we show here that HBHA can be found at two different locations and dynamically alternates between the mycobacterial surface and the interior of the cell, where it participates in the formation of intracytosolic lipid inclusions (ILI)...
2018: Frontiers in Microbiology
W-I Cho, M-S Chung
The sporicidal activities of seven kinds of antimicrobial agent were investigated in order to screen for novel inactivation agents to apply to Clostridium sporogenes spores. Antimicrobial agents based on surfactant components, as poly-l-lysine, thiamine dilaurylsulfate, and torilin, were more effective than other agents. The degree of spore reduction with 1-2% surfactant components was 1.5-2.5 log CFU/mL. The HLB value (hydrophile-lipophile balance) related to denature protein of spores coat on surfactants with sporicidal activity was ranged from 6 to 16...
November 2018: Journal of Food Science and Technology
Tihana Žugčić, Radhia Abdelkebir, Francisco J Barba, Anet Rezek-Jambrak, Fernando Gálvez, Sol Zamuz, Daniel Granato, José M Lorenzo
Physicochemical parameters (pH, colour and texture), proximate composition (moisture, protein, lipid and ash content), amino acid content, and taste profile of beef patties elaborated with soy (control), pulses (pea, lentil and bean) and microalgal ( Chlorella and Spirulina ) proteins were assessed. The pH, colour, ash content, total, essential and non-essential amino acids and amino acid content were significantly different among the beef patties studied. In this regard, beef patties prepared with pea protein presented the highest values for pH; whereas beef patties manufactured with pea also showed the highest values for lightness and patties elaborated with bean the highest values for redness...
November 2018: Journal of Food Science and Technology
Maria Papadaki, Ronald J Holewinski, Samantha Beck Previs, Thomas G Martin, Marisa J Stachowski, Amy Li, Cheavar A Blair, Christine S Moravec, Jennifer E Van Eyk, Kenneth S Campbell, David M Warshaw, Jonathan A Kirk
Patients with diabetes are at significantly higher risk of developing heart failure. Increases in advanced glycation end products are a proposed pathophysiological link, but their impact and mechanism remain incompletely understood. Methylglyoxal (MG) is a glycolysis byproduct, elevated in diabetes, and modifies arginine and lysine residues. We show that left ventricular myofilament from patients with diabetes and heart failure (dbHF) exhibited increased MG modifications compared with nonfailing controls (NF) or heart failure patients without diabetes...
October 18, 2018: JCI Insight
A J M Jansman, O Cirot, E Corrent, W Lambert, J Ensink, J Th M van Diepen
Lowering protein level in diets for piglets urge to have knowledge on the piglet's requirements for essential amino acids (AA) and their interactions. The present studies aimed to determine the interaction between the dietary level of valine (Val) and tryptophan (Trp) and the effect of AA imbalance at two levels of dietary Val on the growth performance of post-weaning piglets. In Experiment 1 (duration 4 weeks), the effects of supplementation of free l-Val (1.0 g/kg) and/or l-Trp (0.5 g/kg) in a low-CP diet (CP 17...
October 18, 2018: Animal: An International Journal of Animal Bioscience
Yan Li, Zhenpeng Zhang, Zhenxing Xu, Dongbo Fang, En Tao Wang, Shuai Shao, Zongjun Du, Wei Liu, Zhihong Xie
A Gram-stain-positive, rod-shaped bacterial strain, 22-7T , was isolated from ocean sediment of Laizhou Bay, China, and was characterized by using a polyphasic approach. Optimal growth was observed at 33 °C on a 2216E agar plate of pH 7.5 and with 2 % (w/v) NaCl. Phylogenetic analysis based on 16S rRNA gene sequences identified it as a member of the genus Jeotgalibacillus, most similar to Jeotgalibacillus campisalis SF-57T (98.7 % similarity), Jeotgalibacillus marinus DSM 1297T (98.2 %) and Jeotgalibacillus soli P9T (97...
October 17, 2018: International Journal of Systematic and Evolutionary Microbiology
Dina Lakayan, Rob Haselberg, Rabah Gahoual, Govert W Somsen, Jeroen Kool
Monoclonal antibodies (mAbs) and antibody-drug conjugates (ADCs) are highly potent biopharmaceuticals designed for targeted cancer therapies. mAbs and ADCs can undergo modifications during production and storage which may affect binding to target receptors, potentially altering drug efficacy. In this work, liquid chromatography was coupled online to surface plasmon resonance (LC-SPR) to allow label-free affinity evaluation of mAb and ADC sample constituents (size and charge variants), under near-native conditions...
October 17, 2018: Analytical and Bioanalytical Chemistry
Markus Buchner, Paula García Calavia, Verena Muhr, Anna Kröninger, Antje J Baeumner, Thomas Hirsch, David A Russell, María J Marín
Photodynamic therapy (PDT) is a well-established treatment of cancer in which cell toxic reactive oxygen species, including singlet oxygen (1O2), are produced by a photosensitiser drug following irradiation of a specific wavelength. Visible light is commonly used as the excitation source in PDT, although these wavelengths do have limited tissue penetration. In this research, upconverting nanoparticles (UCNPs) functionalised with the photosensitiser Rose Bengal (RB) have been designed and synthesised for PDT of breast cancer cells...
October 17, 2018: Photochemical & Photobiological Sciences
Emily Camilleri, Amel Dudakovic, Scott M Riester, Catalina Galeano-Garces, Christopher R Paradise, Elizabeth W Bradley, Meghan E McGee-Lawrence, Hee-Jeong Im, Marcel Karperien, Aaron J Krych, Jennifer J Westendorf, A Noelle Larson, Andre J van Wijnen
Ezh2 is a histone methyltransferase that suppresses osteoblast maturation and skeletal development. We evaluated the role of Ezh2 in chondrocyte lineage differentiation and endochondral ossification.Ezh2 was genetically inactivated in the mesenchymal, osteoblastic, and chondrocytic lineages in mice using the Prrx1-Cre, Osx1-Cre, and Col2a1-Cre drivers, respectively. Wild-type and conditional knockout mice were phenotypically assessed by gross morphology, histology, and micro-CT imaging. Ezh2-deficient chondrocytes in micromass culture models were evaluated using RNA-sequencing, histologic evaluation, and western blotting...
October 16, 2018: Journal of Biological Chemistry
Wentao Jiang, Xiaoli Etienne, Kawang Li, Cangliang Shen
This study was conducted to compare the efficacy of antimicrobials sprayed by electrostatic versus conventional sprayer for inactivation of Salmonella, Listeria monocytogenes, and Campylobacter jejuni on eggs and to determine the economic feasibility of these treatments. Eggs were dip inoculated with overnight cultures (18 h) of Salmonella Typhimurium, Salmonella Tennessee, a two-strain mixture of L. monocytogenes, and a three-strain mixture of C. jejuni (microaerophilic condition). Inoculated eggs were then not sprayed or subjected to electrostatic and conventional spraying with peroxyacetic acid (PAA; 0...
October 16, 2018: Journal of Food Protection
Cristina Avonto, Yan-Hong Wang, Amar G Chittiboyina, Stanislav Vukmanovic, Ikhlas A Khan
Twenty-four pure fragrance ingredients of concern as potential skin sensitizers were previously subjected to degradation studies and evaluated using the high throughput with dansyl cysteamine (HTS-DCYA) method. The experimental results showed that two-thirds of the 24 fragrance ingredients underwent chemical degradation. In some cases, such degradation was accompanied by an increase in thio-reactivity. These results prompted us to investigate the reactivity of the same ingredients using the direct peptide reactivity assay (DPRA)...
October 16, 2018: Journal of Applied Toxicology: JAT
I Ruiz-Ascacibar, P Stoll, G Bee, P Schlegel
Mineral composition and relative deposition rates in the pig's body are used to assess the mineral net requirements for growth and input-output balances. The study aimed to examine the dynamics of changes in mineral composition and deposition rates in the empty body (EB) from birth to 140 kg BW depending on dietary protein supply. In the experiment, 66 entire male, 58 castrated and 66 female Swiss Large White pigs were used to determine body composition at birth, 10, 20 kg and at 20 kg intervals from 40 to 140 kg BW...
October 16, 2018: Animal: An International Journal of Animal Bioscience
Kuo-Tai Yang, Yi-Ling Lin, Yu-Xuen Lin, Sheng-Yao Wang, Yi-Hsieng Samuel Wu, Chung-Hsi Chou, Shih-Guei Fu, Yi-Chen Chen
BACKGROUND: Reactive oxygen species (ROS) overproduction is highly related to some human chronic diseases. There are approximately 400 metric tons of chalazae produced yearly after the processing of the liquid-egg production, which are disposed as waste. The objectives of this study were to look for the optimal production condition of antioxidant crude chalaza hydrolysates and evaluate the in vivo antioxidant capacity via a chronic alcohol-consumption mouse model. RESULTS: Antioxidant crude chalaza hydrolysates (CCH-As) could be produced by protease A at 1:100 ratio (w/w) and 0...
October 16, 2018: Journal of the Science of Food and Agriculture
Xiaoyun Sun, Ji Chen, Yanyong Zhang, Mumingjiang Munisha, Scott Dougan, Yuhua Sun
MAX giant associated protein (MGA) is a dual transcriptional factor containing both T-box and bHLHzip DNA binding domains. In vitro studies have shown that MGA functions as a transcriptional repressor or activator to regulate transcription of promotors containing either E-box or T-box binding sites. BS69 (ZMYND11), a multidomain-containing (i.e., PHD, BROMO, PWWP, and MYND) protein, has been shown to selectively recognizes histone variant H3.3 lysine 36 trimethylation (H3.3K36me3), modulates RNA Polymerase II elongation, and functions as RNA splicing regulator...
2018: Frontiers in Cell and Developmental Biology
Yu-Feng Tian, Hui-Ching Wang, Chi-Wen Luo, Wen-Chun Hung, Yu-Han Lin, Tzu-Yi Chen, Chien-Feng Li, Chen-Yi Lin, Mei-Ren Pan
Pancreatic ductal adenocarcinoma (PDAC) is an aggressive disease, which is characterized by its high invasiveness, rapid progression, and profound resistance to therapy. Gemcitabine is the first-line treatment option for pancreatic cancer patients, but the overall survival is quite low. Therefore, it is an urgent issue to identify new molecules for improved therapies, with better efficacy and less toxicity. Our previous data indicated that Euchromatic histone-lysine N-methyltransferase 2 (EHMT2) functions as a therapeutic target to override GEM resistance and promote metastasis in the treatment of pancreatic cancer...
2018: American Journal of Cancer Research
Michael T Taylor, Jennifer E Nelson, Marcos G Suero, Matthew J Gaunt
Nature has a remarkable ability to carry out site-selective post-translational modification of proteins, therefore enabling a marked increase in their functional diversity1 . Inspired by this, chemical tools have been developed for the synthetic manipulation of protein structure and function, and have become essential to the continued advancement of chemical biology, molecular biology and medicine. However, the number of chemical transformations that are suitable for effective protein functionalization is limited, because the stringent demands inherent to biological systems preclude the applicability of many potential processes2 ...
October 15, 2018: Nature
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